FastQCFastQC Report
Mon 14 Oct 2024
CAFS2_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAFS2_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151444
Sequences flagged as poor quality0
Sequence length225-301
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT49753.285042656031272No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT46673.08166715089406No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT44622.9463035841631227No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT44462.935738622857294No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT44142.914608700245635No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT43862.8961200179604343No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT42782.8248065291460867No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT37032.4451282322178494No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC30972.0449803227595678No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT30211.994796756556879No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT30181.992815826312036No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC28101.8554713293362564No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT27991.8482079184384987No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT27951.8455666781120414No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT27911.842925437785584No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT27751.8323604764797548No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT27491.8151924143577822No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC27391.8085893135416393No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC27211.7967037320725812No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC26841.7722722590528512No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC26491.7491614061963499No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC25401.6771876073003882No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT23311.5391828002429941No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC22491.4850373735506195No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT17451.152241092416999No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT16361.0802672935210376No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT16341.0789466733578088No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT16001.0564961305829217No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT15971.0545152003380787No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT15931.0518739600116214No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT15481.0221600063389769No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT13340.880853648873511No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC10260.6774781437362986No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC10090.666252872348855No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC9860.6510657404717255No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC9570.63191674810491No Hit
CCTACGGGCGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC9400.6206914767174665No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC9240.6101265154116373No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC9000.5942790734528935No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC8830.58305380206545No Hit
CCTACGGGTGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC7640.5044769023533452No Hit
CCTACGGGGGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC7550.4985341116188162No Hit
CCTACGGGTGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC7440.4912707007210586No Hit
CCTACGGGAGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC7340.4846675999049153No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC7120.47014077810940014No Hit
CCTACGGGAGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC7010.4628773672116426No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC6930.457594886558728No Hit
CCTACGGGGGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC6920.45693457647711366No Hit
CCTACGGGCGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC6690.44174744459998416No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC6370.4206175219883257No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC6360.4199572119067114No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC6310.4166556614986398No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC6210.4100525606824965No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC6200.40939225060088213No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC6010.39684635905021No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC6000.3961860489685957No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC5920.39090356831568107No Hit
CCTACGGGCGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC5850.38628139774438075No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC5830.3849607775811521No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC5830.3849607775811521No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC5770.38099891709146616No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC5420.3578880642349647No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC5160.34072000211299225No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC4920.3248725601542484No Hit
CCTACGGGTGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA4340.28657457542061754No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4140.273368373788331No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC3840.2535590713399012No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3810.25157814109505827No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC3810.25157814109505827No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3780.24959721085021525No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC3730.24629566044214363No Hit
CCTACGGGGGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA3700.24431473019730066No Hit
CCTACGGGCGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA3690.24365442011568633No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3580.23639100921792874No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3580.23639100921792874No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC3570.23573069913631445No Hit
CCTACGGGTGGCAGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA3500.23110852856501413No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3500.23110852856501413No Hit
CCTACGGGAGGCAGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA3440.22714666807532816No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC3440.22714666807532816No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC3410.2251657378304852No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3390.22384511766725654No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC3350.22120387734079924No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3230.21328015636142733No Hit
CCTACGGGAGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA3160.20865798579012704No Hit
CCTACGGGGGGCAGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA3150.2079976757085127No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3120.20601674546366974No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3070.20271519505559812No Hit
CCTACGGGCGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC3010.19875333456591215No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2990.19743271440268348No Hit
CCTACGGGCGGCAGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA2960.19545178415784054No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2860.18884868334169724No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2800.1848868228520113No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2790.18422651277039698No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2730.18026465228071104No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC2690.1776234119542537No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2640.17432186154618207No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2580.17036000105649612No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC2520.16639814056681018No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2500.16507752040358153No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC2460.1624362800771242No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2410.15913472966905257No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2400.15847441958743827No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC2400.15847441958743827No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2350.15517286917936662No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2350.15517286917936662No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2340.1545125590977523No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2310.15253162885290933No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC2300.151871318771295No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2230.1472491481999947No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC2210.14592852803676606No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2100.13866511713900848No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC2090.13800480705739415No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2050.13536356673093686No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2030.13404294656770818No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1970.13008108607802224No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC1960.12942077599640792No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC1910.1261192255883363No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1840.12149705501703599No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1690.11159240379282111No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT1670.11027178362959245No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT1630.10763054330313515No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT1590.10498930297667786No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1590.10498930297667786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)