FastQCFastQC Report
Mon 14 Oct 2024
CAFS1_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAFS1_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163941
Sequences flagged as poor quality0
Sequence length38-301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA42962.6204549197577176No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA42552.595445922618503No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA41532.5332284175404567No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA36282.2129912590505123No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA36232.2099413813506077No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA34872.1269847079132127No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA34552.1074654906338255No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA32621.9897402114175222No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA30591.8659151768014102No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG29951.826876742242636No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG28831.7585594817647814No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG27941.704271658706486No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG24551.4974899506529789No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG24431.4901702441732085No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG23871.4560116139342814No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG23231.416973179375507No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG22881.3956240354761773No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG22311.3608554296972692No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG22181.352925747677518No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG20921.2760688296399314No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG20591.2559396368205635No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG19981.2187311288817317No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG19921.2150712756418467No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG19871.2120213979419425No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG19631.1973819849824021No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG19211.1717630123032068No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG18921.154073721643762No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG17401.0613574395666734No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG17231.050987855386999No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG17151.046108051067152No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG17121.0442781244472097No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG16741.0210990539279374No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG16541.0088995431283205No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG15990.975350888429374No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG15700.9576615977699295No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG15280.9320426250907338No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9820.5989959802611915No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA9640.5880164205415362No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9510.5800867385217853No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA9410.5739869831219768No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9230.5630074234023216No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA9120.5562976924625322No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8580.5233590133035666No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8340.5087196003440262No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8230.502009869404237No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8180.49895999170433264No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8070.49225026076454337No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7910.48249065212484976No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7900.48188067658486894No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7800.4757809211850605No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG7690.46907119024527116No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7430.45321182620576916No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7390.4507719240458457No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7270.44345221756607556No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG7220.4404023398661714No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG7130.43491256000634376No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7110.43369260892638206No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6950.4239330002866885No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG6630.40441378300730146No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6600.4025838563873589No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6470.39465417436760786No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG6270.3824546635679909No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA6180.37696488370816333No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG6180.37696488370816333No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG6120.37330503046827823No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG5980.3647653729085464No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA5970.3641553973685655No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5940.362325470748623No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA5790.35317583764891025No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5730.3495159844090252No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5670.3458561311691401No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5640.3440262045491976No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA5580.3403663513093125No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5580.3403663513093125No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5510.3360965225294466No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5440.3318266937495806No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA5440.3318266937495806No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA5400.3293867915896572No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5360.3269468894297339No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5350.326336913889753No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5240.31962718294996373No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5220.31840723187000197No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG5210.3177972563300212No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG5050.3080376476903276No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG5050.3080376476903276No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG5040.3074276721503468No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5020.3062077210703851No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4960.30254786783050003No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA4920.30010796567057657No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG4880.2976680635106532No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA4850.29583813689071065No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA4750.2897383814909022No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG4750.2897383814909022No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG4670.28485857717105545No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4660.2842486016310746No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG4640.2830286505511129No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG4460.2720490908314577No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG4430.2702191642115151No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4430.2702191642115151No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG4410.26899921313155345No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4360.26594933543164917No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4340.2647293843516875No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4100.25008997139214717No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4090.24947999585216632No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4080.24887002031218547No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4070.24826004477220462No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG4060.24765006923222377No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3980.24277026491237702No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG3970.24216028937239617No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3910.2385004361325111No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG3910.2385004361325111No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3900.23789046059253025No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG3800.2317907051927218No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3750.22874082749281752No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG3730.22752087641285584No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG3720.226910900872875No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG3680.22447099871295162No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3550.21654131669320062No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG3440.20983158575341132No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG3410.20800165913346874No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG3400.2073916835934879No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG3220.19641212387383264No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG3200.19519217279387097No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG3120.1903123684740242No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG3070.18726249077411994No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2840.1732330533545605No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2830.17262307781457964No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2800.1707931511946371No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2780.16957320011467542No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG2770.16896322457469456No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG2750.16774327349473286No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG2690.1640834202548478No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2650.1616435180949244No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2570.15676371377507764No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG2440.1488340317553266No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG2150.13114474109588203No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2100.1280948633959778No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG1880.11467540151639918No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG1820.11101554827651411No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG1780.10857564611659073No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG1700.10369584179674395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)