FastQCFastQC Report
Mon 14 Oct 2024
CAFS1_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAFS1_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163941
Sequences flagged as poor quality0
Sequence length38-301
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT57653.516508987989582No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT57373.4994296728701175No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT54333.3139971087159403No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT53243.2475097748580284No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT52883.2255506554187177No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT51813.1602832726407675No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT51403.1352742755015526No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT43482.652173647836722No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT31881.9446020214589397No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT30941.8872643207007398No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT29751.8146772314430193No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT29251.7841784544439767No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC28781.7555096040648768No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT28611.7451400198852027No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT28251.723180900445892No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT28001.7079315119463712No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC25731.5694670643707185No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC25521.556657578031121No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC25321.544458067231504No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC25131.5328685319718678No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC24411.488950293093247No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC24381.4871203664733044No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT24341.484680464313381No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC20641.2589895145204677No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT20121.2272707864414636No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT19741.2040917159221916No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT17951.09490609426562No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT17891.0912462410257349No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT17801.0857564611659072No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT17771.0839265345459648No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT17101.043058173367248No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT14310.8728749977125918No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC10350.6313246838801764No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC10230.6240049774004063No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC9230.5630074234023216No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC9010.5495879615227429No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC8970.5471480593628195No Hit
CCTACGGGCGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC8860.5404383284230302No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC8850.5398283528830493No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC8780.5355585241031835No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC8570.5227490377635857No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC8480.517259257903758No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC8430.5142093802038539No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC8400.5123794535839112No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC8240.5026198449442177No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC8010.4885904075246582No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC7500.45748165498563503No Hit
CCTACGGGGGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC7420.4526018506657883No Hit
CCTACGGGAGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC7350.4483320218859224No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC7200.4391823887862097No Hit
CCTACGGGTGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC7190.4385724132462288No Hit
CCTACGGGGGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC7000.4269828779865928No Hit
CCTACGGGCGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC6930.4227130492067268No Hit
CCTACGGGAGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC6590.401973880847378No Hit
CCTACGGGTGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC6570.4007539297674163No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC6450.39343422328764616No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC6440.3928242477476653No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC6350.3873344678878377No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC6310.3848945657279143No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC6220.3794047858680867No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC5870.35805564196875705No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC5790.35317583764891025No Hit
CCTACGGGCGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC5690.3470760822491018No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC4840.2952281613507299No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC4740.2891284059509214No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4350.26533935989166835No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4250.25923960449185984No Hit
CCTACGGGCGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA4070.24826004477220462No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC4060.24765006923222377No Hit
CCTACGGGGGGCAGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA4040.2464301181522621No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC4000.2439902159923387No Hit
CCTACGGGAGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA3980.24277026491237702No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3970.24216028937239617No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC3970.24216028937239617No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC3940.24033036275245362No Hit
CCTACGGGTGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA3940.24033036275245362No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC3920.23911041167249195No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC3900.23789046059253025No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3890.2372804850525494No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3860.23545055843260687No Hit
CCTACGGGGGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA3850.23484058289262602No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC3840.23423060735264517No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC3820.23301065627268344No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3820.23301065627268344No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3760.22935080303279837No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3710.22630092533289414No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC3700.2256909497929133No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC3650.22264107209300907No Hit
CCTACGGGTGGCAGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA3590.218981218853124No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC3500.2134914389932964No Hit
CCTACGGGAGGCAGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA3490.21288146345331554No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC3490.21288146345331554No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3380.20617173251352622No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3320.20251187927364114No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC3290.2006819526536986No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3280.20007197711371774No Hit
CCTACGGGCGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC3220.19641212387383264No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3180.19397222171390927No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3120.1903123684740242No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3110.18970239293404334No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3080.18787246631410082No Hit
CCTACGGGCGGCAGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA3020.18421261307421571No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC2980.18177271091429234No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2950.1799427842943498No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2840.1732330533545605No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2800.1707931511946371No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2780.16957320011467542No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2610.15920361593500099No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2560.15615373823509676No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2520.1537138360751734No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2500.1524938849952117No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2500.1524938849952117No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2500.1524938849952117No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2470.15066395837526916No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2330.1421243008155373No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2330.1421243008155373No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2310.14090434973557558No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2220.135414569875748No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2180.13297466771582458No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2180.13297466771582458No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGAT1810.11040557273653327No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)