##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IVMS2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	105923
Sequences flagged as poor quality	0
Sequence length	328-488
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.248133077801796	34.0	34.0	34.0	20.0	34.0
2	33.149297130934734	34.0	34.0	34.0	34.0	34.0
3	33.60623282950823	34.0	34.0	34.0	34.0	34.0
4	33.3764432653909	34.0	34.0	34.0	34.0	34.0
5	33.338274029247664	34.0	34.0	34.0	34.0	34.0
6	33.10349027123476	34.0	34.0	34.0	34.0	34.0
7	33.541619855933085	34.0	34.0	34.0	34.0	34.0
8	33.48899672403538	34.0	34.0	34.0	34.0	34.0
9	33.49497276323367	34.0	34.0	34.0	34.0	34.0
10-19	33.47665285159975	34.0	34.0	34.0	34.0	34.0
20-29	33.708577929250495	34.0	34.0	34.0	34.0	34.0
30-39	33.598251560095534	34.0	34.0	34.0	34.0	34.0
40-49	33.47043512740387	34.0	34.0	34.0	34.0	34.0
50-59	33.50887342692333	34.0	34.0	34.0	34.0	34.0
60-69	33.46333940692767	34.0	34.0	34.0	34.0	34.0
70-79	33.517177572387496	34.0	34.0	34.0	34.0	34.0
80-89	33.64921877212692	34.0	34.0	34.0	34.0	34.0
90-99	33.24550192120692	34.0	34.0	34.0	34.0	34.0
100-109	33.42302521643081	34.0	34.0	34.0	34.0	34.0
110-119	33.276534841347015	34.0	34.0	34.0	34.0	34.0
120-129	33.42503611113734	34.0	34.0	34.0	34.0	34.0
130-139	33.38391284234775	34.0	34.0	34.0	34.0	34.0
140-149	33.35840374611746	34.0	34.0	34.0	34.0	34.0
150-159	33.12300822295441	34.0	34.0	34.0	33.1	34.0
160-169	33.206633120285495	34.0	34.0	34.0	34.0	34.0
170-179	33.011040095163466	34.0	34.0	34.0	33.1	34.0
180-189	33.064195689321494	34.0	34.0	34.0	34.0	34.0
190-199	33.09034204091652	34.0	34.0	34.0	34.0	34.0
200-209	33.21360516601682	34.0	34.0	34.0	34.0	34.0
210-219	33.25085769851685	34.0	34.0	34.0	34.0	34.0
220-229	33.27416991588229	34.0	34.0	34.0	34.0	34.0
230-239	33.30896594696147	34.0	34.0	34.0	34.0	34.0
240-249	33.300890269346596	34.0	34.0	34.0	34.0	34.0
250-259	33.214342494075886	34.0	34.0	34.0	33.8	34.0
260-269	33.363874701434064	34.0	34.0	34.0	34.0	34.0
270-279	33.34594847200325	34.0	34.0	34.0	34.0	34.0
280-289	33.31071061053784	34.0	34.0	34.0	34.0	34.0
290-299	33.19982534482596	34.0	34.0	34.0	34.0	34.0
300-309	31.735325661093434	34.0	34.0	34.0	22.8	34.0
310-319	31.136198936963645	34.0	34.0	34.0	20.0	34.0
320-329	31.723738302486435	34.0	34.0	34.0	20.0	34.0
330-339	31.840318347463228	34.0	34.0	34.0	22.8	34.0
340-349	32.03376352410264	34.0	34.0	34.0	20.0	34.0
350-359	32.2239553633806	34.0	34.0	34.0	25.6	34.0
360-369	32.04170623609887	34.0	34.0	34.0	24.2	34.0
370-379	32.26249451047092	34.0	34.0	34.0	27.0	34.0
380-389	32.53500218676122	34.0	34.0	34.0	34.0	34.0
390-399	32.24397265577052	34.0	34.0	34.0	31.2	34.0
400-409	32.61945254888631	34.0	34.0	34.0	32.6	34.0
410-419	32.9075593911697	34.0	34.0	34.0	34.0	34.0
420-429	32.83027648405185	34.0	34.0	34.0	34.0	34.0
430-439	32.83482870747837	34.0	34.0	34.0	34.0	34.0
440-449	32.94223221950928	34.0	34.0	34.0	34.0	34.0
450-459	32.94165481788401	34.0	34.0	34.0	34.0	34.0
460-469	32.50922461568015	34.0	34.0	34.0	30.0	34.0
470-479	33.05333333333333	NaN	NaN	NaN	NaN	NaN
480-488	32.44444444444444	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.14194306853720207
1101	2	0.02123009227635464
1101	3	0.02194676427571096
1101	4	0.024867197966322863
1101	5	0.27806383371294885
1101	6	-0.09619067630787015
1101	7	0.02812136980623592
1101	8	0.06172774663483693
1101	9	0.04606158164995122
1101	10-19	-0.044738933756583776
1101	20-29	-0.076880294303308
1101	30-39	0.06485709254555161
1101	40-49	-0.02844861401081289
1101	50-59	-0.03988110243495413
1101	60-69	-0.019082573991397567
1101	70-79	-0.040003178527300065
1101	80-89	-0.02547642468172029
1101	90-99	-0.18298263272354376
1101	100-109	-0.05367014845710827
1101	110-119	-0.10600312716474036
1101	120-129	-0.021322973796500833
1101	130-139	0.027925823471200317
1101	140-149	-0.05500187090570563
1101	150-159	-0.012860012065019077
1101	160-169	-0.33463667615175297
1101	170-179	-0.39785405429809373
1101	180-189	-0.4895743061507076
1101	190-199	-0.40711274186524804
1101	200-209	-0.3678824414396118
1101	210-219	-0.3335455095119144
1101	220-229	-0.3522862382962444
1101	230-239	-0.44658942876228735
1101	240-249	-0.2791528441265214
1101	250-259	-0.25548996778350386
1101	260-269	-0.2593446973043285
1101	270-279	-0.326773177084867
1101	280-289	-0.37580850133782207
1101	290-299	-0.4117017512325347
1101	300-309	-0.9456679279892128
1101	310-319	-1.030792311126973
1101	320-329	-1.1037736159273415
1101	330-339	-0.8932454876476683
1101	340-349	-0.8291438070219712
1101	350-359	-0.7183376216414139
1101	360-369	-0.6310450993982961
1101	370-379	-0.5610141959469033
1101	380-389	-0.5759416714926466
1101	390-399	-0.6853234227733189
1101	400-409	-0.6977738393366799
1101	410-419	-0.2764764426091162
1101	420-429	-0.41616746100206115
1101	430-439	-0.6022472090033091
1101	440-449	-0.5210830237168267
1101	450-459	-0.2875087750128884
1101	460-469	0.32061876742484685
1101	470-479	31.919354838709676
1101	480-488	31.39784946236559
1102	1	0.09526298805430145
1102	2	0.017403209410531417
1102	3	-0.0600984481754665
1102	4	-0.02780964741971559
1102	5	0.1401224599803612
1102	6	0.16683058853609367
1102	7	-0.05647246772878134
1102	8	0.26018910300837206
1102	9	0.07093237503772798
1102	10-19	-0.05698732607125123
1102	20-29	0.058438283832167315
1102	30-39	0.023828962983870383
1102	40-49	-0.05481623148033066
1102	50-59	-0.06725791231383482
1102	60-69	-0.15190065897186855
1102	70-79	-0.10438634024269788
1102	80-89	-0.047045841180725745
1102	90-99	-0.13132523737102986
1102	100-109	-0.16504585366876512
1102	110-119	-0.08580349801732012
1102	120-129	-0.09649715614548882
1102	130-139	0.0012946639651474356
1102	140-149	-0.1597795566278748
1102	150-159	-0.16588956191312576
1102	160-169	-0.26619149526249686
1102	170-179	-0.4946047545782122
1102	180-189	-0.6185670863613879
1102	190-199	-0.5465469944395167
1102	200-209	-0.33997144605554297
1102	210-219	-0.3877097104035414
1102	220-229	-0.2805152200297769
1102	230-239	-0.2948689095686987
1102	240-249	-0.21608897068982458
1102	250-259	-0.36954054576116846
1102	260-269	-0.4974367792075114
1102	270-279	-0.4831656495668213
1102	280-289	-0.42565660959521523
1102	290-299	-0.5587826681063248
1102	300-309	-1.316869253589882
1102	310-319	-1.473437989116487
1102	320-329	-1.3851110523103465
1102	330-339	-0.9385889207955813
1102	340-349	-0.8355650797564245
1102	350-359	-0.6712732747759205
1102	360-369	-0.6772422824968842
1102	370-379	-0.5723985806867198
1102	380-389	-0.5918796523187879
1102	390-399	-0.4349569099710102
1102	400-409	-0.5700782540603129
1102	410-419	-0.2776308198529591
1102	420-429	-0.4207044870800942
1102	430-439	-0.49338414638519623
1102	440-449	-0.25595343987114916
1102	450-459	-0.14790439790850485
1102	460-469	0.22327700183977583
1102	470-479	-2.0806451612903225
1102	480-488	-1.046594982078853
1103	1	-0.015720114864286927
1103	2	0.02899171578081905
1103	3	0.024725804106090266
1103	4	-0.023110993764710486
1103	5	-0.21275094744712675
1103	6	-0.12270135327288756
1103	7	7.020149675227572E-4
1103	8	0.0381551281281034
1103	9	-0.22686739002958944
1103	10-19	-0.06978663934495444
1103	20-29	-0.054772161636336136
1103	30-39	-0.00920166862361782
1103	40-49	-0.11893930006259978
1103	50-59	-0.06562016043888974
1103	60-69	0.022889256994524487
1103	70-79	-0.08884915762770618
1103	80-89	-0.014864581580098957
1103	90-99	-0.0172450891827296
1103	100-109	-0.02912600185707248
1103	110-119	-0.08262738886397614
1103	120-129	-0.09609731879120176
1103	130-139	-0.0211662558866621
1103	140-149	-0.05593705188405096
1103	150-159	-0.17245186425341785
1103	160-169	-0.257225650860228
1103	170-179	-0.5197357747282041
1103	180-189	-0.4510758963369881
1103	190-199	-0.5162881166948665
1103	200-209	-0.2806622385015558
1103	210-219	-0.33276934716147366
1103	220-229	-0.3287938863350135
1103	230-239	-0.26743374195326197
1103	240-249	-0.2840793926650633
1103	250-259	-0.3592170615287671
1103	260-269	-0.3942651879903849
1103	270-279	-0.27804456887647433
1103	280-289	-0.35541625538902366
1103	290-299	-0.3261292841721044
1103	300-309	-0.9873626122236558
1103	310-319	-1.193314649458035
1103	320-329	-0.9410543973903138
1103	330-339	-0.9160805626135904
1103	340-349	-0.5387818503356172
1103	350-359	-0.692351706148461
1103	360-369	-0.7777799169054838
1103	370-379	-0.5614064418957163
1103	380-389	-0.40635511992517337
1103	390-399	-0.6088793821857053
1103	400-409	-0.42077853417236355
1103	410-419	-0.2919413827878188
1103	420-429	-0.5440373686198043
1103	430-439	-0.5067698421873104
1103	440-449	-0.395110582059381
1103	450-459	-0.36521206979031007
1103	460-469	-0.4887961035709303
1103	470-479	-2.0806451612903225
1103	480-488	-1.046594982078853
1104	1	0.08875098154229732
1104	2	0.043259705855263064
1104	3	-0.004890040025884446
1104	4	-0.1899155154079395
1104	5	-0.01968505276833099
1104	6	0.09811934335426287
1104	7	0.037807979492846755
1104	8	-0.06847569624466132
1104	9	-0.14982943395937554
1104	10-19	-0.030079810928441475
1104	20-29	0.0262038515977423
1104	30-39	0.0070054814545059685
1104	40-49	-0.011279158531486644
1104	50-59	0.013244374070808362
1104	60-69	-0.12639849229322664
1104	70-79	-0.08026079258773677
1104	80-89	-0.12376541790028739
1104	90-99	-0.3253854255488733
1104	100-109	-0.21514520965047268
1104	110-119	-0.31148043545895376
1104	120-129	-0.21227919545693652
1104	130-139	-0.330721903345534
1104	140-149	-0.4122347267301123
1104	150-159	-0.33490246622014297
1104	160-169	-0.37016334041081933
1104	170-179	-0.9135805500245908
1104	180-189	-0.7337314427022079
1104	190-199	-0.7551011853466463
1104	200-209	-0.5186757482892546
1104	210-219	-0.5649607817722568
1104	220-229	-0.4601053466787306
1104	230-239	-0.5515137897429909
1104	240-249	-0.5595733729977539
1104	250-259	-0.45482867810812877
1104	260-269	-0.5485249067662323
1104	270-279	-0.5653366376201561
1104	280-289	-0.5749778765635369
1104	290-299	-0.6429958405547893
1104	300-309	-1.8375831801861615
1104	310-319	-1.651165490373394
1104	320-329	-1.4226484604359904
1104	330-339	-1.4662505837513606
1104	340-349	-1.3021216169011964
1104	350-359	-1.3556707944675495
1104	360-369	-1.3014588067134056
1104	370-379	-0.8073718862482551
1104	380-389	-0.9309852090713946
1104	390-399	-1.1943310869922534
1104	400-409	-0.8025449159387463
1104	410-419	-0.5641567122612159
1104	420-429	-0.8154853053903253
1104	430-439	-0.6873755977626459
1104	440-449	-0.4999886396609412
1104	450-459	-0.7742580242621315
1104	460-469	0.003939342192701645
1104	470-479	-2.0806451612903225
1104	480-488	-1.046594982078853
1201	1	-0.5394817685741096
1201	2	-0.12031725273554628
1201	3	0.02784587082636847
1201	4	0.09573450708418818
1201	5	0.09874191884424022
1201	6	-0.11088382132757602
1201	7	-0.37520272991675796
1201	8	-0.30793399110461905
1201	9	0.3386837969361238
1201	10-19	-0.09631241898931364
1201	20-29	-0.10589359850525426
1201	30-39	-0.17403799543385645
1201	40-49	-0.15155032678049452
1201	50-59	-0.09090759379306945
1201	60-69	-0.12503318067860647
1201	70-79	-0.10441970538931145
1201	80-89	-0.06204386254992045
1201	90-99	-0.20922897504338778
1201	100-109	-0.1444828362180246
1201	110-119	-0.1682363603589181
1201	120-129	-0.09503917683188234
1201	130-139	-0.07712118991103267
1201	140-149	0.01891252962490597
1201	150-159	-0.20204186216841435
1201	160-169	-0.30332147190690506
1201	170-179	-0.433643527982305
1201	180-189	-0.4113229698782348
1201	190-199	-0.41247031667700895
1201	200-209	-0.3770440036104219
1201	210-219	-0.34991631049509664
1201	220-229	-0.4864503364586241
1201	230-239	-0.3366374175258926
1201	240-249	-0.44801320852899806
1201	250-259	-0.4924655052186395
1201	260-269	-0.47096968267360495
1201	270-279	-0.5364583239772998
1201	280-289	-0.4688481798521167
1201	290-299	-0.44140718605754614
1201	300-309	-1.7258801709762324
1201	310-319	-1.9609128682648276
1201	320-329	-1.7943896668032409
1201	330-339	-1.3075469195075335
1201	340-349	-1.355119383472367
1201	350-359	-1.1914514291401517
1201	360-369	-1.5620068254331656
1201	370-379	-1.0723512487345452
1201	380-389	-1.0777964567136706
1201	390-399	-1.320662660415234
1201	400-409	-0.7657462165361686
1201	410-419	-0.7253305645707968
1201	420-429	-0.7332288319981259
1201	430-439	-0.5804859038923382
1201	440-449	-0.5055850146312864
1201	450-459	-0.5214031826655727
1201	460-469	-0.09585869190476615
1201	470-479	-2.0806451612903225
1201	480-488	-1.046594982078853
1202	1	-0.26992149633548124
1202	2	-0.13206717476963092
1202	3	-0.3979580953455937
1202	4	0.059136389273106715
1202	5	-0.17559720536922185
1202	6	-0.6137823188785276
1202	7	-0.0056534646308534775
1202	8	-0.3076221274440698
1202	9	0.12457104905038108
1202	10-19	-0.09001735927658672
1202	20-29	-0.028474362020872945
1202	30-39	-0.1179355573938281
1202	40-49	-0.06155839854582723
1202	50-59	-0.2028006062125769
1202	60-69	-0.05889882247567613
1202	70-79	-0.07842403479071436
1202	80-89	-0.032239786308430496
1202	90-99	-0.19227001263213594
1202	100-109	-0.1418494227785274
1202	110-119	-0.14639581441406335
1202	120-129	-0.11276220521148161
1202	130-139	-0.12341826756508567
1202	140-149	-0.23165983191680084
1202	150-159	-0.2514823420715473
1202	160-169	-0.3102182450352515
1202	170-179	-0.5314302132384938
1202	180-189	-0.47273259362390263
1202	190-199	-0.682219541604077
1202	200-209	-0.41490883823095714
1202	210-219	-0.3743719249582398
1202	220-229	-0.24215285521630392
1202	230-239	-0.28434430237482644
1202	240-249	-0.308013025079795
1202	250-259	-0.2267631699101713
1202	260-269	-0.24917866801602173
1202	270-279	-0.23455136941767307
1202	280-289	-0.3839607259437443
1202	290-299	-0.3083606449924403
1202	300-309	-1.1392105079724288
1202	310-319	-1.4950325872206243
1202	320-329	-1.2678702487041384
1202	330-339	-1.1098959866682812
1202	340-349	-1.1445462881338067
1202	350-359	-0.8690073352875309
1202	360-369	-0.9785667858081482
1202	370-379	-0.7325308455131747
1202	380-389	-0.6238513814077677
1202	390-399	-0.694238635906423
1202	400-409	-0.6991449800884766
1202	410-419	-0.3129963567962193
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2402	210-219	0.361563161684586
2402	220-229	0.328648828976398
2402	230-239	0.3647564741311129
2402	240-249	0.33142268572993316
2402	250-259	0.46827100058260385
2402	260-269	0.4188544657249622
2402	270-279	0.33129189063775755
2402	280-289	0.4056971459645169
2402	290-299	0.4028849382333419
2402	300-309	1.3520839506950324
2402	310-319	1.5286794073628691
2402	320-329	1.280529133476417
2402	330-339	1.2771689478470911
2402	340-349	1.1962245050871019
2402	350-359	0.9987824791550608
2402	360-369	1.02403567277468
2402	370-379	1.0100680161756657
2402	380-389	0.7770520737262743
2402	390-399	0.8562173233005765
2402	400-409	0.7251213593313679
2402	410-419	0.5313649829409997
2402	420-429	0.5420245022792187
2402	430-439	0.5993615202843827
2402	440-449	0.6187743923212565
2402	450-459	0.624256710799024
2402	460-469	0.673412075475099
2402	470-479	-2.0806451612903225
2402	480-488	-1.046594982078853
2404	1	0.5343809171051177
2404	2	0.18376908461967645
2404	3	-0.14661359114541028
2404	4	0.2562784844773347
2404	5	-0.03574654533989019
2404	6	0.45042053458162457
2404	7	0.03550392014180659
2404	8	-0.05654430176292635
2404	9	-0.0950387113834168
2404	10-19	0.059527064920047223
2404	20-29	0.02345905724219932
2404	30-39	0.07287958994605503
2404	40-49	0.06056925157195536
2404	50-59	0.10691538116462596
2404	60-69	-0.0042919510591659105
2404	70-79	0.13337426735604652
2404	80-89	0.017000179912287194
2404	90-99	0.21135237058489764
2404	100-109	0.004021504818247479
2404	110-119	0.06674476961188702
2404	120-129	0.0050519198607545945
2404	130-139	0.004129623452293174
2404	140-149	0.10359062365900229
2404	150-159	0.19149956823558512
2404	160-169	0.2536334817081567
2404	170-179	0.4341593685206888
2404	180-189	0.4026640906731842
2404	190-199	0.43441008637937983
2404	200-209	0.31557251649592644
2404	210-219	0.30619832260831714
2404	220-229	0.3195787773006984
2404	230-239	0.2439125090390064
2404	240-249	0.25627205425706734
2404	250-259	0.43614629273261585
2404	260-269	0.30531264270889835
2404	270-279	0.2501235050211932
2404	280-289	0.24710954384602246
2404	290-299	0.3410659961916096
2404	300-309	1.3200632375273713
2404	310-319	1.5831286643905464
2404	320-329	1.2211181507802813
2404	330-339	1.209931561222728
2404	340-349	1.0800281373962122
2404	350-359	0.9813060119723431
2404	360-369	1.191885234281635
2404	370-379	0.9498261481309811
2404	380-389	0.7677193550694099
2404	390-399	0.9802668976728484
2404	400-409	0.6730693405479968
2404	410-419	0.3986752730539891
2404	420-429	0.6102149948066184
2404	430-439	0.5714094650492711
2404	440-449	0.5216190967909782
2404	450-459	0.5365746707810928
2404	460-469	0.7875917352455772
2404	470-479	-2.0806451612903225
2404	480-488	-1.046594982078853
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	30.0
19	59.0
20	133.0
21	174.0
22	218.0
23	314.0
24	369.0
25	473.0
26	663.0
27	815.0
28	1031.0
29	1382.0
30	2092.0
31	4350.0
32	15378.0
33	76501.0
34	1936.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.1010167763375282	0.03965144491753444	0.1076253504904506	99.75170642825448
2	0.026434296611689622	0.11895433475260331	0.25301398185474355	99.60159738678097
3	0.00944082021846058	0.2313000953522842	99.68750885076896	0.07175023366030041
4	0.09724044825014397	99.7007259990748	0.028322460655381736	0.17371109201967466
5	0.1642702718012141	0.029266542677227794	0.18787232234736556	99.61859086317419
6	99.77058806869141	0.05853308535445559	0.01982572245876722	0.15105312349536928
7	99.88576607535663	0.059477167376301654	0.043427773004918666	0.011328984262152696
8	99.84328238437355	0.06702982355107012	0.043427773004918666	0.04626001907045684
9	30.057683411534796	19.451865978116178	27.633280779434116	22.85716983091491
10-19	41.80045882386262	23.37499881989747	14.259792490771597	20.56474986546831
20-29	31.350698148655155	21.59106143141716	30.02926654267723	17.028973877250454
30-39	23.583924171332004	37.62582253146153	10.03936822031098	28.750885076895482
40-49	29.29184407541327	31.659696194405367	18.240608744087687	20.80785098609367
50-59	38.27478451327851	29.763696269931934	9.63312972631062	22.328389490478934
60-69	39.93797381116471	10.538315568856623	18.992003625274965	30.5317069947037
70-79	39.94316626228487	38.76004267250739	18.964719654843613	2.3320714103641325
80-89	30.935396467245074	9.40494510163043	40.628192177336366	19.03146625378813
90-99	19.452526835531472	29.88746542299595	30.69323942864156	19.966768312831018
100-109	38.3950605628617	23.76537673593082	34.30756304107701	3.531999660130472
110-119	39.31761751460967	47.81161787336084	10.70409637189279	2.166668240136703
120-129	30.596754246008896	28.909585264767802	28.738989643420222	11.754670845803084
130-139	22.61718418096164	14.743068077754595	41.312368418568205	21.327379322715558
140-149	28.314813591004786	29.69355097570877	27.979758881451623	14.011876551834824
150-159	14.720126884623735	52.739442802790705	2.671280080813421	29.869150231772135
160-169	30.59014567185597	20.204110533123117	18.919309309592816	30.286434485428092
170-179	24.889023158331998	20.418889193093097	19.575540722978012	35.1165469255969
180-189	38.365605203780106	19.92437903005013	5.059713187881763	36.650302578288
190-199	20.36177223077141	38.48512598774582	29.18044239683544	11.972659384647338
200-209	48.53594130736688	20.610207767465088	12.104930736310827	18.7489201888572
210-219	41.28432918251937	11.842848106643505	28.310093180895556	18.56272952994156
220-229	32.499077157209925	9.986055869044124	47.11539641644331	10.399470557302637
230-239	38.49239541931403	38.785438478895045	12.805056503309006	9.917109598481916
240-249	45.233424279901435	23.64000264342966	5.0360167291334275	26.090556347535475
250-259	20.469760323860648	44.70870573751842	20.454938110177196	14.366595828443735
260-269	30.25886729039019	38.32765310650189	27.86495850759514	3.5485210955127777
270-279	27.099024763271434	7.267732220575324	21.109013151062562	44.52422986509068
280-289	28.59511154329088	38.391378642976505	13.989407399714887	19.02410241401773
290-299	33.50320515846417	21.409042417605242	18.87861937445125	26.209133049479338
300-309	17.99514741840771	29.790319382947988	43.075252777961346	9.13928042068295
310-319	28.660469077036222	13.081590477602104	38.207318719559225	20.050621725802447
320-329	49.30491895520232	44.618585782677776	4.2232605036304705	1.8532347584894298
330-339	29.940522271105152	30.565038424501047	21.00743943656653	18.48699986782727
340-349	31.450436595265003	23.49051518146861	28.364861279867526	16.69418694339886
350-359	44.719269575035945	22.982027323905633	18.956136549665366	13.342566551393054
360-369	25.923635611790285	36.70930532254669	33.22501371303734	4.14204535262568
370-379	28.774682659944578	44.17930598237341	5.63862178352428	21.407389574157733
380-389	37.18852054279777	30.44981065523391	12.203508566540687	20.158160235427626
390-399	39.75006845499438	3.499891416215808	18.84296896392186	37.90707116486795
400-409	32.29291183940931	21.7966367353105	27.189662823744914	18.72078860153528
410-419	31.056766249952787	24.16890131057144	16.796464856290367	27.977867583185407
420-429	37.53139529174488	31.5641181558264	13.07413254572854	17.83035400670018
430-439	38.436816573693136	19.588775158354153	20.151812157253588	21.82259611069912
440-449	29.14414200503816	39.54725015985838	20.43359996642698	10.875007868676482
450-459	16.563532354884433	33.308109756441894	16.749772705454504	33.378585183219165
460-469	32.923845535271276	16.767517770135527	33.459753660961596	16.84888303363161
470-479	20.833333333333336	22.916666666666664	31.25	25.0
480-488	33.33333333333333	8.333333333333332	33.33333333333333	25.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	3.2000000000000006
48	12.199999999999989
49	117.1500000000011
50	1483.8000000002003
51	15594.60000002167
52	5455.1999999972295
53	519.4499999999812
54	1647.9000000002377
55	416.1999999999849
56	281.5999999999926
57	35.99999999999997
58	15.799999999999976
59	3.600000000000001
60	2.4
61	3.800000000000001
62	1.4
63	0.4
64	1.5999999999999999
65	0.4
66	0.0
67	0.2
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0016049394371382983
210-219	0.0
220-229	2.832246065538174E-4
230-239	0.0
240-249	0.0
250-259	8.496738196614521E-4
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	9.44082021846058E-5
320-329	0.0
330-339	0.0
340-349	9.440909348388437E-5
350-359	9.440909348388437E-5
360-369	0.0
370-379	0.0
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-488	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
325-329	1.0
330-334	0.0
335-339	0.0
340-344	0.0
345-349	0.0
350-354	0.0
355-359	0.0
360-364	1.0
365-369	1.0
370-374	1.0
375-379	0.0
380-384	0.0
385-389	10.0
390-394	0.0
395-399	0.0
400-404	0.0
405-409	1.0
410-414	0.0
415-419	0.0
420-424	0.0
425-429	1.0
430-434	2.0
435-439	7612.0
440-444	9680.0
445-449	36.0
450-454	39.0
455-459	10.0
460-464	3945.0
465-469	84576.0
470-474	3.0
475-479	2.0
480-484	1.0
485-489	1.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.149240486013425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.43230944254835	2.50748185002313
2	12.127417519908988	1.0063914352878978
3	5.847554038680318	0.7278872388433107
4	3.981797497155859	0.6608574152922405
5	2.6848691695108076	0.5570083928891743
6	1.888509670079636	0.4701528468793369
7	1.61547212741752	0.46920876485749086
8	1.0466439135381116	0.3474221840393493
9	0.7508532423208191	0.2803923604882792
>10	8.168373151308304	7.004144520075904
>50	0.477815699658703	1.3292674867592498
>100	0.38680318543799774	3.750837872794388
>500	0.3640500568828214	11.887880819085563
>1k	0.06825938566552901	5.0517828988982565
>5k	0.09101251422070535	31.263276153432212
>10k+	0.06825938566552901	32.686007760354215
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	13462	12.709232178091634	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	10913	10.302767104406032	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	10247	9.674008477856557	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	8986	8.483521048308678	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	8663	8.178582555252401	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	7775	7.3402377198531	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	7691	7.260934830018032	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	2890	2.7283970431351072	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1276	1.20464865987557	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1185	1.1187371958875787	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	966	0.911983233103292	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	948	0.8949897567100631	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	939	0.8864930185134484	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	938	0.8855489364916024	No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	893	0.8430652455085298	No Hit
CCTACGGGGGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	884	0.8345685073119153	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	807	0.7618741916297688	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	792	0.7477129613020779	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	754	0.7118378444719277	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	725	0.684459465838392	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	704	0.6646337433796249	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	684	0.6457521029427036	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	668	0.6306467905931668	No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	642	0.6061006580251692	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	639	0.603268411959631	No Hit
CCTACGGGCGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	609	0.5749459513042493	No Hit
CCTACGGGGGGCAGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	396	0.37385648065103894	No Hit
CCTACGGGAGGCAGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	393	0.37102423458550077	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	308	0.29077726272858584	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	289	0.27283970431351073	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	278	0.2624548020732041	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	262	0.2473494897236672	No Hit
CCTACGGGCGGCAGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	262	0.2473494897236672	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	260	0.24546132567997508	No Hit
CCTACGGGTGGCAGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	217	0.20486579874059457	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	214	0.2020335526750564	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	199	0.18787232234736556	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	191	0.1803196661725971	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	187	0.17654333808521283	No Hit
CCTACGGGCGGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGATGGA	158	0.14916495945167715	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	136	0.1283951549710639	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	116	0.10951351453414272	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	107	0.1010167763375282	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0
320-329	0.0	0.0	0.0	0.0	0.0
330-339	0.0	0.0017937558415075101	0.0	0.0	0.0
340-349	0.0	0.001888164043692116	0.0	0.0	0.0
350-359	0.0	0.001888164043692116	0.0	0.0	0.0
360-369	0.0	0.001888164043692116	0.0	0.0	0.0
370-379	0.0	0.001888164043692116	0.0	0.0	0.0
380-389	0.0	0.001888164043692116	0.0	0.0	0.0
390-399	0.0	0.001888164043692116	0.0	0.0	0.0
400-409	0.0	0.001888164043692116	0.0	0.0	0.0
410-419	0.0	0.001888164043692116	0.0	0.0	0.0
420-429	0.0	0.001888164043692116	0.0	0.0	0.0
430-439	0.0	0.001888164043692116	0.0	0.0	0.0
440-449	0.0	0.001888164043692116	0.0	0.0	0.0
450-459	0.0	0.001888164043692116	0.0	0.0	0.0
460-469	0.0	0.001888164043692116	0.0	0.0	0.0
470-477	0.0	0.0018881640436921158	0.0	0.0	0.0
>>END_MODULE
