##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IVFS2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	122876
Sequences flagged as poor quality	0
Sequence length	323-488
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09694325987174	34.0	34.0	34.0	20.0	34.0
2	33.01039259090465	34.0	34.0	34.0	34.0	34.0
3	33.54963540479833	34.0	34.0	34.0	34.0	34.0
4	33.31580617858654	34.0	34.0	34.0	34.0	34.0
5	33.16532113675575	34.0	34.0	34.0	34.0	34.0
6	32.99825026856343	34.0	34.0	34.0	34.0	34.0
7	33.472053126729385	34.0	34.0	34.0	34.0	34.0
8	33.43080829454084	34.0	34.0	34.0	34.0	34.0
9	33.440126631726294	34.0	34.0	34.0	34.0	34.0
10-19	33.41046095250497	34.0	34.0	34.0	34.0	34.0
20-29	33.70925160324229	34.0	34.0	34.0	34.0	34.0
30-39	33.59558660763697	34.0	34.0	34.0	34.0	34.0
40-49	33.417734952309644	34.0	34.0	34.0	34.0	34.0
50-59	33.49170301767636	34.0	34.0	34.0	34.0	34.0
60-69	33.42661138057879	34.0	34.0	34.0	32.6	34.0
70-79	33.5066774634591	34.0	34.0	34.0	34.0	34.0
80-89	33.64483544386211	34.0	34.0	34.0	34.0	34.0
90-99	33.098793092223055	34.0	34.0	34.0	34.0	34.0
100-109	33.42098212832449	34.0	34.0	34.0	34.0	34.0
110-119	33.233288844037894	34.0	34.0	34.0	34.0	34.0
120-129	33.44341938214134	34.0	34.0	34.0	34.0	34.0
130-139	33.36366418177675	34.0	34.0	34.0	34.0	34.0
140-149	33.40594583157004	34.0	34.0	34.0	34.0	34.0
150-159	33.1096707249585	34.0	34.0	34.0	32.6	34.0
160-169	33.213374458804	34.0	34.0	34.0	34.0	34.0
170-179	33.00557472573976	34.0	34.0	34.0	32.6	34.0
180-189	33.05142745532081	34.0	34.0	34.0	34.0	34.0
190-199	33.06616914613106	34.0	34.0	34.0	34.0	34.0
200-209	33.21779354796705	34.0	34.0	34.0	34.0	34.0
210-219	33.29500716169146	34.0	34.0	34.0	34.0	34.0
220-229	33.30550310882516	34.0	34.0	34.0	34.0	34.0
230-239	33.32868013281682	34.0	34.0	34.0	34.0	34.0
240-249	33.312698167258056	34.0	34.0	34.0	34.0	34.0
250-259	33.18356066278199	34.0	34.0	34.0	33.8	34.0
260-269	33.3710472346105	34.0	34.0	34.0	34.0	34.0
270-279	33.3349750968456	34.0	34.0	34.0	34.0	34.0
280-289	33.33036231648166	34.0	34.0	34.0	34.0	34.0
290-299	33.21427211172239	34.0	34.0	34.0	34.0	34.0
300-309	31.808304306780816	34.0	34.0	34.0	22.8	34.0
310-319	31.225632344802886	34.0	34.0	34.0	20.0	34.0
320-329	31.728996362559336	34.0	34.0	34.0	20.0	34.0
330-339	31.90999641909599	34.0	34.0	34.0	22.8	34.0
340-349	31.975161547601612	34.0	34.0	34.0	20.0	34.0
350-359	32.212300405293234	34.0	34.0	34.0	25.6	34.0
360-369	31.997646369451633	34.0	34.0	34.0	24.2	34.0
370-379	32.25072594690496	34.0	34.0	34.0	28.4	34.0
380-389	32.56566268962117	34.0	34.0	34.0	34.0	34.0
390-399	32.22072824344231	34.0	34.0	34.0	31.2	34.0
400-409	32.65824238428921	34.0	34.0	34.0	32.6	34.0
410-419	32.97521221443627	34.0	34.0	34.0	34.0	34.0
420-429	32.87551867688228	34.0	34.0	34.0	32.6	34.0
430-439	32.87350895097141	34.0	34.0	34.0	34.0	34.0
440-449	33.03914635618351	34.0	34.0	34.0	34.0	34.0
450-459	33.023499769521734	34.0	34.0	34.0	34.0	34.0
460-469	32.6028823538209	34.0	34.0	34.0	30.0	34.0
470-479	32.633752913752915	NaN	NaN	NaN	NaN	NaN
480-488	31.90740740740741	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.38265445116450536
1101	2	0.3187621622717245
1101	3	0.23244692423960345
1101	4	0.07285292278739774
1101	5	-0.009925960851745685
1101	6	0.260531875235678
1101	7	0.16684554362345239
1101	8	-0.06652544334121302
1101	9	-0.07083078528957998
1101	10-19	0.03988562711349175
1101	20-29	0.06390326604106633
1101	30-39	0.10096285289628071
1101	40-49	-0.0403597513767906
1101	50-59	0.040684740679431286
1101	60-69	-0.015415573224707657
1101	70-79	0.01838259918432783
1101	80-89	0.02414980623122176
1101	90-99	-0.09683554985338105
1101	100-109	-0.006311829106586231
1101	110-119	-0.05796808959961908
1101	120-129	-0.015857487939925363
1101	130-139	-0.08968452327955134
1101	140-149	0.07033021829907682
1101	150-159	-0.07635023298887234
1101	160-169	-0.17668588214090875
1101	170-179	-0.5199782673689484
1101	180-189	-0.337583951131613
1101	190-199	-0.3446206849718578
1101	200-209	-0.34651748527608817
1101	210-219	-0.25228543538634085
1101	220-229	-0.23418781054935067
1101	230-239	-0.23951025800651848
1101	240-249	-0.17496601651237143
1101	250-259	-0.16672831788633857
1101	260-269	-0.17363214821617134
1101	270-279	-0.19535937154559235
1101	280-289	-0.22497042181327487
1101	290-299	-0.3049069703034277
1101	300-309	-0.8305870783958831
1101	310-319	-0.7922304586749647
1101	320-329	-0.8877959442450845
1101	330-339	-0.755335287331615
1101	340-349	-0.6555432054710444
1101	350-359	-0.7134939877299296
1101	360-369	-0.49594178285887125
1101	370-379	-0.584459096118394
1101	380-389	-0.47429558045320164
1101	390-399	-0.784337244497145
1101	400-409	-0.4421743517399932
1101	410-419	-0.2856625216752917
1101	420-429	-0.36819801896718474
1101	430-439	-0.41645328027524187
1101	440-449	-0.40679994140838716
1101	450-459	-0.31895136467908003
1101	460-469	-1.0138110201248054
1101	470-479	-3.053763440860215
1102	1	-0.3457213099403482
1102	2	-0.2881279727967012
1102	3	0.058807418941931644
1102	4	-0.1084514305424733
1102	5	0.2640844446066879
1102	6	-0.07781008711732085
1102	7	-0.16685700204283904
1102	8	0.10750015256749634
1102	9	0.21500285396254526
1102	10-19	-0.04484860330033058
1102	20-29	-0.00376639230038478
1102	30-39	-0.05001566527529633
1102	40-49	-0.006494541305521295
1102	50-59	-0.03956957277551254
1102	60-69	0.017018569844019282
1102	70-79	-0.04015063625499238
1102	80-89	0.020144590843287347
1102	90-99	-0.048895754671285374
1102	100-109	0.05194734529244727
1102	110-119	-0.18584762932897547
1102	120-129	0.018524830375952206
1102	130-139	-0.06425444365626731
1102	140-149	0.046961609364565504
1102	150-159	-0.19741305815943377
1102	160-169	-0.3077161744304888
1102	170-179	-0.6892619110516165
1102	180-189	-0.612565912471922
1102	190-199	-0.5854007145932272
1102	200-209	-0.394350314370854
1102	210-219	-0.2962541936936205
1102	220-229	-0.3480680844838062
1102	230-239	-0.29067802387105246
1102	240-249	-0.27067598018187766
1102	250-259	-0.2752014244600076
1102	260-269	-0.35889677851858437
1102	270-279	-0.3585554422824586
1102	280-289	-0.4358341254982321
1102	290-299	-0.5821086361894743
1102	300-309	-1.314734577668009
1102	310-319	-1.6019852594901849
1102	320-329	-1.5690445992573032
1102	330-339	-1.1267721773400972
1102	340-349	-1.1884773675278275
1102	350-359	-0.8718006322860532
1102	360-369	-0.8299519351431286
1102	370-379	-0.5678913783569541
1102	380-389	-0.6435937816507185
1102	390-399	-0.6856221034158416
1102	400-409	-0.6580135608238784
1102	410-419	-0.49711485530821875
1102	420-429	-0.5418584111339726
1102	430-439	-0.42624974853421094
1102	440-449	-0.3472629630864077
1102	450-459	-0.3516124395802507
1102	460-469	-0.00413948622357907
1102	470-479	-3.053763440860215
1103	1	-0.5051890135681631
1103	2	0.31319694686985855
1103	3	-0.12748466879615705
1103	4	0.1489299320989872
1103	5	-0.2525876552396298
1103	6	-0.20154687284824035
1103	7	-0.311608550499038
1103	8	-0.4239346725617352
1103	9	-0.5641616782406871
1103	10-19	-0.09743906574153272
1103	20-29	-0.01818566301244573
1103	30-39	-0.09071677830096547
1103	40-49	-0.1319066867905505
1103	50-59	-0.10243223751727726
1103	60-69	-0.0330652563563234
1103	70-79	-0.08870347004626211
1103	80-89	-0.09192754625910737
1103	90-99	-0.13110480790092538
1103	100-109	-0.12161130976274848
1103	110-119	-0.3104389766022919
1103	120-129	-0.14483264179840205
1103	130-139	-0.09999933258655602
1103	140-149	-0.0481648284876286
1103	150-159	-0.07407095412181519
1103	160-169	-0.23900313945632945
1103	170-179	-0.4516553819220164
1103	180-189	-0.4375507771857414
1103	190-199	-0.4954540373706706
1103	200-209	-0.2911719042744565
1103	210-219	-0.4086651283557572
1103	220-229	-0.4111507930014753
1103	230-239	-0.4640503984810209
1103	240-249	-0.4049003549781247
1103	250-259	-0.3587190978172501
1103	260-269	-0.4149823278130427
1103	270-279	-0.2825239143171103
1103	280-289	-0.3389578157138473
1103	290-299	-0.4370462322403128
1103	300-309	-1.085471083978259
1103	310-319	-1.0974667944410506
1103	320-329	-1.0610531681577768
1103	330-339	-0.974989706329989
1103	340-349	-0.9372397668199675
1103	350-359	-0.744539653382315
1103	360-369	-0.8890610489139164
1103	370-379	-0.7253728667377111
1103	380-389	-0.5960445337580147
1103	390-399	-0.7562840975480078
1103	400-409	-0.7405614116132426
1103	410-419	-0.413962757324704
1103	420-429	-0.24982422867228138
1103	430-439	-0.5220550185742283
1103	440-449	-0.4542111029916214
1103	450-459	-0.2051662279829003
1103	460-469	-0.4394399589187046
1103	470-479	-3.053763440860215
1104	1	0.11061603819948829
1104	2	0.4890241679259688
1104	3	0.04883782359789279
1104	4	0.10986835336263567
1104	5	0.07913801160785283
1104	6	0.21658005812037118
1104	7	0.027386992058922033
1104	8	0.15043857335227528
1104	9	0.28261080354040047
1104	10-19	-0.023178435096028238
1104	20-29	0.014420289677346432
1104	30-39	-0.003270137891533409
1104	40-49	-0.09040943436725968
1104	50-59	-0.05003960526804008
1104	60-69	-0.06152331159086799
1104	70-79	7.384063245723382E-4
1104	80-89	-0.0092826793927685
1104	90-99	-0.1590245503598382
1104	100-109	0.11886238189547527
1104	110-119	-0.13608577738529704
1104	120-129	-0.10081540163896818
1104	130-139	-0.02915076769421887
1104	140-149	-0.06624223232243764
1104	150-159	-0.13271638701254318
1104	160-169	-0.2505423720215987
1104	170-179	-0.6443165219894595
1104	180-189	-0.5343209005318954
1104	190-199	-0.6777121209064347
1104	200-209	-0.5103271128780307
1104	210-219	-0.2941819793734126
1104	220-229	-0.3618506585706811
1104	230-239	-0.3105793718542955
1104	240-249	-0.19957736731274167
1104	250-259	-0.2013448613605604
1104	260-269	-0.18124550556828467
1104	270-279	-0.25925618478241574
1104	280-289	-0.3736533417627754
1104	290-299	-0.3368605054885947
1104	300-309	-1.3611597469814818
1104	310-319	-1.4656497382395521
1104	320-329	-1.2384546445380522
1104	330-339	-1.0911243493808627
1104	340-349	-1.063223234191021
1104	350-359	-0.9376488960888523
1104	360-369	-0.8571243021390735
1104	370-379	-0.6699779468065792
1104	380-389	-0.6085011975159489
1104	390-399	-0.7323265257926259
1104	400-409	-0.3144063681001228
1104	410-419	-0.17466238705041803
1104	420-429	-0.4090964156490031
1104	430-439	-0.3909457124339255
1104	440-449	-0.41957394207566523
1104	450-459	-0.23192493530838476
1104	460-469	0.002436349999491938
1104	470-479	-3.053763440860215
1201	1	-0.7767266929525363
1201	2	0.08721885429481091
1201	3	-0.26077216908502265
1201	4	-0.1249648752866861
1201	5	0.31135521906998775
1201	6	-0.4120938651991253
1201	7	-0.20951833258024521
1201	8	-0.2341165411634023
1201	9	-0.026759603043686297
1201	10-19	-0.08373479286930774
1201	20-29	-0.025642391226341488
1201	30-39	-0.06666656223089262
1201	40-49	-0.23328110039719263
1201	50-59	-0.11547963427739205
1201	60-69	-0.19680592605798353
1201	70-79	-0.1743660078874214
1201	80-89	-0.11993519537058006
1201	90-99	-0.3050568103042721
1201	100-109	-0.1684505628766928
1201	110-119	-0.19061945865808383
1201	120-129	-0.19826607604146318
1201	130-139	-0.05295267531759151
1201	140-149	6.184774999482556E-4
1201	150-159	-0.2336352320780435
1201	160-169	-0.3269211252673543
1201	170-179	-0.5969451700115869
1201	180-189	-0.6328238111792288
1201	190-199	-0.5809536560705624
1201	200-209	-0.4037987154500229
1201	210-219	-0.46966390357355436
1201	220-229	-0.3343228633455766
1201	230-239	-0.3444186419584696
1201	240-249	-0.36083823772256807
1201	250-259	-0.5506626885088934
1201	260-269	-0.4173446553613971
1201	270-279	-0.33159435084797195
1201	280-289	-0.4726930547774799
1201	290-299	-0.7127406606367828
1201	300-309	-2.1062317199406344
1201	310-319	-2.196395110018898
1201	320-329	-1.8042579378756187
1201	330-339	-1.8128684066743155
1201	340-349	-1.8296721149354163
1201	350-359	-1.7681507965893957
1201	360-369	-1.7347327148487928
1201	370-379	-1.3727603832029445
1201	380-389	-1.3289605552956907
1201	390-399	-1.389851795650749
1201	400-409	-1.1294944645561813
1201	410-419	-0.7385768353872351
1201	420-429	-0.8046459419808016
1201	430-439	-0.8270137807871905
1201	440-449	-0.6115931344785892
1201	450-459	-0.6481269479558733
1201	460-469	0.23942652287325927
1201	470-479	28.612903225806452
1202	1	0.6517280542854138
1202	2	0.11131666709876242
1202	3	0.26117460997576103
1202	4	-0.2070969549933963
1202	5	0.05093673389652764
1202	6	-0.20166041631267717
1202	7	0.0580889697116973
1202	8	0.13867319372948117
1202	9	0.13431229759726193
1202	10-19	-0.20935204603039637
1202	20-29	3.1965082168738945E-4
1202	30-39	-0.06874838880145262
1202	40-49	-0.020143583874755677
1202	50-59	-0.07708025277752739
1202	60-69	-0.07801711392740884
1202	70-79	-0.04785280241070211
1202	80-89	0.051566413228570696
1202	90-99	-0.20760812337008616
1202	100-109	-0.029884086581709823
1202	110-119	-0.2490028788640828
1202	120-129	-0.08036947529826222
1202	130-139	0.038441942733633994
1202	140-149	-0.009294358871862585
1202	150-159	-0.1366061525957889
1202	160-169	-0.2740063186733366
1202	170-179	-0.4552706058297247
1202	180-189	-0.45730506912870084
1202	190-199	-0.40738518461383677
1202	200-209	-0.3051672717011087
1202	210-219	-0.2853932598845006
1202	220-229	-0.20073431557391785
1202	230-239	-0.2033432497845027
1202	240-249	-0.19620746734073435
1202	250-259	-0.27015292468617247
1202	260-269	-0.26313611108128043
1202	270-279	-0.3096812401414297
1202	280-289	-0.45508325853337084
1202	290-299	-0.4038983532322433
1202	300-309	-1.0844900684455077
1202	310-319	-1.217255149423341
1202	320-329	-0.9687945615631719
1202	330-339	-0.9392325985091183
1202	340-349	-0.870526168854667
1202	350-359	-0.7727165995172918
1202	360-369	-0.8328229709776807
1202	370-379	-0.8324375410950537
1202	380-389	-0.7997869263458988
1202	390-399	-0.9627606167595602
1202	400-409	-0.5662472141384498
1202	410-419	-0.5391149055670539
1202	420-429	-0.6264842341223584
1202	430-439	-0.576986970801407
1202	440-449	-0.5406724996013281
1202	450-459	-0.4289305452681944
1202	460-469	0.258827186713539
1202	470-479	-3.053763440860215
1203	1	0.546581841989763
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2404	10-19	0.16373427422954023
2404	20-29	0.11112887127862336
2404	30-39	0.09974561176138508
2404	40-49	0.20120558592594762
2404	50-59	0.1726216941613643
2404	60-69	0.06871389638293124
2404	70-79	0.22813921539573556
2404	80-89	0.033823941306970084
2404	90-99	0.3286852734114518
2404	100-109	0.12910995235706224
2404	110-119	0.34030302847083505
2404	120-129	0.13063674160248695
2404	130-139	0.16183718923502255
2404	140-149	0.1602153246546365
2404	150-159	0.3352841178266104
2404	160-169	0.4105185873056385
2404	170-179	0.6926948302487617
2404	180-189	0.5823494195759835
2404	190-199	0.6519305725432716
2404	200-209	0.4547944802880437
2404	210-219	0.37446551669084016
2404	220-229	0.4186864637133141
2404	230-239	0.4072075727396083
2404	240-249	0.38053927710114266
2404	250-259	0.5382411705373045
2404	260-269	0.4540460722533268
2404	270-279	0.3901742105199233
2404	280-289	0.4531675665491406
2404	290-299	0.4880260717684024
2404	300-309	1.5497534140422786
2404	310-319	1.8770543396651007
2404	320-329	1.6475427116990389
2404	330-339	1.3555374017467265
2404	340-349	1.324683875405583
2404	350-359	1.2215302088844737
2404	360-369	1.3358411628775642
2404	370-379	1.1118079881090281
2404	380-389	0.8678052398474634
2404	390-399	1.0021938438027718
2404	400-409	0.7400445196860872
2404	410-419	0.6047136051910087
2404	420-429	0.6750981334167179
2404	430-439	0.6340019769305769
2404	440-449	0.5646699009708271
2404	450-459	0.5283799807277134
2404	460-469	0.7066835105978768
2404	470-479	-3.053763440860215
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	9.0
18	28.0
19	79.0
20	142.0
21	208.0
22	228.0
23	287.0
24	372.0
25	598.0
26	693.0
27	943.0
28	1202.0
29	1618.0
30	2556.0
31	5110.0
32	18506.0
33	88236.0
34	2061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.11719131482144601	0.0398776001822976	0.10498388619421205	99.73794719880205
2	0.03255367286814135	0.11556553868190178	0.2840307957745333	99.56784999267543
3	0.007324457176340376	0.2531006868713174	99.6573781698623	0.08219668609004199
4	0.09521794329242488	99.68586216999252	0.030111657280510434	0.1888082294345519
5	0.17497314365702007	0.02767017155506364	0.16113805787948826	99.63621862690843
6	99.74283017025294	0.06510628601191445	0.031739314430808296	0.16032422930433934
7	99.86490445652528	0.05696800026042515	0.05696800026042515	0.021159542953872196
8	99.83479279924477	0.04638822878348905	0.05696800026042515	0.061850971711318724
9	30.455906767798428	22.042546957908787	25.27507405840034	22.226472215892446
10-19	40.358084573065526	24.914954913896935	14.605781438197857	20.121179074839677
20-29	30.04923662879651	20.228116149614245	29.956704319802075	19.765942901787167
30-39	20.834906735245287	39.359761059930335	10.056642468830365	29.74868973599401
40-49	29.993733519971354	30.334890458673787	19.728750935902863	19.942625085452
50-59	39.665923369901364	29.967202708421496	9.849685862169991	20.517188059507145
60-69	40.09798496044793	10.212653406686416	19.77066310752303	29.918698525342624
70-79	40.29924476708226	39.55687034083141	19.60366548390247	0.5402194081838602
80-89	30.134118949184547	9.854487450763372	39.79336892476969	20.218024675282397
90-99	19.94628731404017	29.684478661414758	30.27564373840294	20.093590286142128
100-109	39.863358182232496	20.83580194667795	38.59272762785247	0.7081122432370845
110-119	39.667632409909174	49.425355643087336	10.357921807350499	0.5490901396529835
120-129	30.080975943227315	29.95857612552492	29.61896546111527	10.341482470132492
130-139	20.210618835248543	10.92109118135356	48.540072919040334	20.328217064357563
140-149	29.725821153032328	29.850011393600052	29.474348123311305	10.949819330056316
150-159	11.066034050587584	58.3857710211921	1.2585858914678212	29.289609036752502
160-169	29.861404993652137	20.229743806764546	19.804843907679288	30.104007291904033
170-179	20.819199843744915	20.17033432077867	19.744783358833295	39.26568247664312
180-189	39.873775187994404	19.780591816139847	0.8742960382824962	39.471336957583254
190-199	20.284758618444613	39.48134704905758	29.815749210586283	10.41814512191152
200-209	49.738227565007705	20.07220410518426	10.463165896093475	19.726402433714565
210-219	39.85261564504053	10.341075555844917	29.925616068231385	19.88069273088317
220-229	30.53240665386243	10.16699762362056	49.095999218724565	10.204596503792441
230-239	39.67585207851818	39.6114782382239	10.210862983821087	10.50180669943683
240-249	49.396057814381976	20.342052150135096	1.1984439597643153	29.063446075718613
250-259	20.134315579760862	48.13948832470124	20.669087559043838	11.057108536494058
260-269	30.40862332758228	39.40737003157655	29.266414922360756	0.9175917184804193
270-279	29.49225235196458	1.0112633874800612	20.824733878055927	48.67175038249943
280-289	29.830316742081447	39.86368371366255	10.015218594355286	20.290780949900714
290-299	30.403252058986297	20.479019499332658	20.014404765780135	29.10332367590091
300-309	11.880513688596633	29.743155701683	48.11232461994205	10.264005989778314
310-319	30.053712685959827	11.162472736742734	38.813031023145285	19.970783554152153
320-329	49.75560528992879	48.89611393692777	0.8532248219735503	0.49505595116988815
330-339	29.956133925810178	29.701808356527827	20.172127545290298	20.169930172371696
340-349	30.713764273779866	20.309520573531074	29.69401964127451	19.282695511414545
350-359	49.12284128456793	20.50222178817325	19.867587935608835	10.507348991649982
360-369	20.9020622751762	39.48378013249345	38.901557693246744	0.7125998990836141
370-379	29.59706691407458	48.825789019646145	0.9331510327652717	20.643993033514004
380-389	40.06699562233415	29.852035057135907	10.29024111750521	19.79072820302473
390-399	39.70098721423281	0.6952820437694818	20.142507182329435	39.46122355966827
400-409	30.216161665486567	20.28477020615117	29.129412147699618	20.369655980662646
410-419	30.642055489090186	20.539346143516372	19.503137436823987	29.315460930569458
420-429	39.2318535555523	30.203434855432338	10.47409534320069	20.090616245814672
430-439	40.165648321256164	19.96024830969855	20.01819917680468	19.855904192240605
440-449	29.727169319417808	40.13392449415066	20.00141147947958	10.137494706951951
450-459	16.68599284931242	33.23387013471682	16.689741462325607	33.39039555364515
460-469	32.814244752066344	16.828738644206652	33.507516286690645	16.849500317036355
470-479	21.11111111111111	22.22222222222222	25.555555555555554	31.11111111111111
480-488	34.61538461538461	11.538461538461538	30.76923076923077	23.076923076923077
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.2
44	0.0
45	0.2
46	0.4
47	1.2
48	6.400000000000003
49	35.14999999999996
50	886.0000000000645
51	21176.200000041976
52	6588.199999996199
53	158.84999999999954
54	456.9999999999826
55	258.1999999999939
56	124.40000000000123
57	11.99999999999999
58	13.449999999999985
59	7.650000000000004
60	3.0000000000000004
61	1.5999999999999999
62	0.8
63	1.0
64	0.4
65	0.2
66	0.2
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0016276571502978612
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0017090400078127541
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	9.765942901787167E-4
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	8.138418217035338E-5
350-359	0.0
360-369	0.0
370-379	0.0
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-488	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
320-324	1.0
325-329	1.0
330-334	0.0
335-339	0.0
340-344	0.0
345-349	0.0
350-354	0.0
355-359	0.0
360-364	0.0
365-369	0.0
370-374	0.0
375-379	0.0
380-384	0.0
385-389	3.0
390-394	0.0
395-399	0.0
400-404	0.0
405-409	0.0
410-414	0.0
415-419	0.0
420-424	0.0
425-429	3.0
430-434	0.0
435-439	1164.0
440-444	1601.0
445-449	41.0
450-454	19.0
455-459	15.0
460-464	6339.0
465-469	113676.0
470-474	4.0
475-479	4.0
480-484	3.0
485-489	2.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	3.0559262996842347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.733688415446075	1.8254174940590513
2	9.533954727030626	0.5827012598066343
3	4.92676431424767	0.4516748592076565
4	3.0093209054593872	0.36785051596731666
5	2.4766977363515315	0.3784302874442527
6	2.157123834886818	0.39552068752238023
7	1.3848202396804261	0.29623360135421073
8	1.411451398135819	0.3450633158631466
9	1.2516644474034622	0.34424948728799765
>10	12.010652463382158	8.002376379439436
>50	1.2782956058588548	2.4341612682704517
>100	0.6125166444740346	3.796510303069761
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.02663115845539281	7.171457404212377
>10k+	0.18641810918774968	73.60835313649532
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	16643	13.544548976203652	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	13627	11.090041993554479	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	12958	10.545590676779842	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	12859	10.465021647840098	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	12208	9.935219245418144	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	11456	9.32322015690615	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	10696	8.704710439792962	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	8812	7.171457404212377	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	428	0.3483186301637423	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	325	0.26449428692340243	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	321	0.26123897262280676	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	315	0.25635600117191315	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	310	0.25228685829616854	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	294	0.2392656010937856	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	294	0.2392656010937856	No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	236	0.1920635437351476	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	211	0.17171782935642435	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	189	0.1538136007031479	No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	183	0.1489306292522543	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	167	0.1359093720498714	No Hit
CCTACGGGGGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	144	0.11719131482144601	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	138	0.11230834337055241	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	138	0.11230834337055241	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	138	0.11230834337055241	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	137	0.11149451479540348	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	128	0.10417005761906312	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0
320-329	0.0	1.6276571502978613E-4	0.0	0.0	0.0
330-339	0.0	0.004801588593378691	0.0	0.0	0.0
340-349	0.0	0.0048829714508935845	0.0	0.0	0.0
350-359	0.0	0.0048829714508935845	0.0	0.0	0.0
360-369	0.0	0.0048829714508935845	0.0	0.0	0.0
370-379	0.0	0.0048829714508935845	0.0	0.0	0.0
380-389	0.0	0.0048829714508935845	0.0	0.0	0.0
390-399	0.0	0.0048829714508935845	0.0	0.0	0.0
400-409	0.0	0.0048829714508935845	0.0	0.0	0.0
410-419	0.0	0.0048829714508935845	0.0	0.0	0.0
420-429	0.0	0.0048829714508935845	0.0	0.0	0.0
430-439	0.0	0.0048829714508935845	0.0	0.0	0.0
440-449	0.0	0.0048829714508935845	0.0	0.0	0.0
450-459	0.0	0.0048829714508935845	0.0	0.0	0.0
460-469	0.0	0.0048829714508935845	0.0	0.0	0.0
470-477	0.0	0.004882971450893584	0.0	0.0	0.0
>>END_MODULE
