FastQCFastQC Report
Tue 15 Oct 2024
IVFS2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIVFS2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122876
Sequences flagged as poor quality0
Sequence length323-488
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT1664313.544548976203652No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT1362711.090041993554479No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT1295810.545590676779842No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT1285910.465021647840098No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT122089.935219245418144No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT114569.32322015690615No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT106968.704710439792962No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT88127.171457404212377No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT4280.3483186301637423No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT3250.26449428692340243No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT3210.26123897262280676No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT3150.25635600117191315No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT3100.25228685829616854No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2940.2392656010937856No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2940.2392656010937856No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2360.1920635437351476No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2110.17171782935642435No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1890.1538136007031479No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT1830.1489306292522543No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1670.1359093720498714No Hit
CCTACGGGGGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT1440.11719131482144601No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1380.11230834337055241No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1380.11230834337055241No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1380.11230834337055241No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1370.11149451479540348No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1280.10417005761906312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)