FastQCFastQC Report
Tue 15 Oct 2024
IVFS1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIVFS1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221417
Sequences flagged as poor quality0
Sequence length319-498
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2546711.50182686966222No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT215169.717411038899453No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT192738.704390358463893No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT168447.607365288121508No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT164467.427613959181093No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT143906.499049305157237No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT142516.436271830979555No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC78063.525474557057498No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC63762.8796343550856527No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC58902.6601390137162007No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC50722.2907003527281105No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC49832.2505047037941983No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT47072.1258530284485837No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC43711.9741031628104435No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC43381.959199158149555No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT21510.97147012198702No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT20060.9059828287800846No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT16050.7248765903250428No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT15040.6792613033326258No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC14240.6431303829425925No Hit
CCTACGGGGGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT10210.46112087147779984No Hit
CCTACGGGAGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9640.4353775906999011No Hit
CCTACGGGGGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC6360.28724081710076466No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC5650.2551746252546101No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5630.25427135224485925No Hit
CCTACGGGCGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5470.24704516816685257No Hit
CCTACGGGTGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5360.242077166613223No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5060.22852807146696053No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC5030.22717316195233428No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4530.20459133670856347No Hit
CCTACGGGCGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC4290.19375206059155348No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3700.16710550680390396No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3630.16394405126977604No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT3600.1625891417551498No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT3330.15039495612351356No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3200.14452368156013315No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3160.14271713554063148No Hit
CCTACGGGCGGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCA3030.13684586097725107No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2920.13187785942362149No Hit
CCTACGGGGGGCAGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2890.13052294990899524No Hit
CCTACGGGAGGCAGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2670.1205869468017361No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2630.11878040078223444No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2610.11787712777248359No Hit
CCTTCGGGTTGTAAAATTCTGTTATAAGGGAAGAACGACTTTAGTAGGAA2430.1097476706847261No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2330.10523130563597194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)