##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IVFS1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	221417
Sequences flagged as poor quality	0
Sequence length	319-498
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3790088385264	34.0	34.0	34.0	20.0	34.0
2	33.15471711747517	34.0	34.0	34.0	34.0	34.0
3	33.65680141994517	34.0	34.0	34.0	34.0	34.0
4	33.42247885212066	34.0	34.0	34.0	34.0	34.0
5	33.27742223948477	34.0	34.0	34.0	34.0	34.0
6	33.21152847342345	34.0	34.0	34.0	34.0	34.0
7	33.58831977671091	34.0	34.0	34.0	34.0	34.0
8	33.52585393172159	34.0	34.0	34.0	34.0	34.0
9	33.4953775003726	34.0	34.0	34.0	34.0	34.0
10-19	33.34750086940028	34.0	34.0	34.0	32.6	34.0
20-29	33.63470465230764	34.0	34.0	34.0	34.0	34.0
30-39	33.57583744698916	34.0	34.0	34.0	34.0	34.0
40-49	33.68772271325147	34.0	34.0	34.0	34.0	34.0
50-59	33.49402259085798	34.0	34.0	34.0	34.0	34.0
60-69	33.31949443809644	34.0	34.0	34.0	34.0	34.0
70-79	33.53856975751636	34.0	34.0	34.0	34.0	34.0
80-89	33.6264103478956	34.0	34.0	34.0	34.0	34.0
90-99	33.545176747946186	34.0	34.0	34.0	34.0	34.0
100-109	33.366110099947164	34.0	34.0	34.0	34.0	34.0
110-119	33.389344991576976	34.0	34.0	34.0	34.0	34.0
120-129	33.31017672536436	34.0	34.0	34.0	34.0	34.0
130-139	33.33100755587873	34.0	34.0	34.0	34.0	34.0
140-149	32.98638361101451	34.0	34.0	34.0	32.6	34.0
150-159	33.094293121124394	34.0	34.0	34.0	34.0	34.0
160-169	33.2195829588514	34.0	34.0	34.0	34.0	34.0
170-179	33.00353541056016	34.0	34.0	34.0	32.6	34.0
180-189	33.04964569116193	34.0	34.0	34.0	33.1	34.0
190-199	33.19573609975747	34.0	34.0	34.0	34.0	34.0
200-209	33.13252550617161	34.0	34.0	34.0	33.1	34.0
210-219	33.19992457670369	34.0	34.0	34.0	33.1	34.0
220-229	33.35777605152269	34.0	34.0	34.0	33.8	34.0
230-239	33.17295419954204	34.0	34.0	34.0	34.0	34.0
240-249	33.359189222146455	34.0	34.0	34.0	34.0	34.0
250-259	33.40588617856804	34.0	34.0	34.0	34.0	34.0
260-269	33.45082130098412	34.0	34.0	34.0	34.0	34.0
270-279	33.43211000058712	34.0	34.0	34.0	34.0	34.0
280-289	33.37162096857965	34.0	34.0	34.0	34.0	34.0
290-299	33.36108745037644	34.0	34.0	34.0	34.0	34.0
300-309	31.901214902198113	34.0	34.0	34.0	22.8	34.0
310-319	31.532686288767348	34.0	34.0	34.0	20.0	34.0
320-329	32.03463661162698	34.0	34.0	34.0	21.4	34.0
330-339	31.847106803483037	34.0	34.0	34.0	21.4	34.0
340-349	32.17040615576605	34.0	34.0	34.0	28.4	34.0
350-359	32.434728552566	34.0	34.0	34.0	31.2	34.0
360-369	32.40345517572846	34.0	34.0	34.0	29.8	34.0
370-379	32.030832463878085	34.0	32.6	34.0	27.3	34.0
380-389	32.716639638999176	34.0	34.0	34.0	34.0	34.0
390-399	32.610779534395746	34.0	34.0	34.0	32.6	34.0
400-409	32.741274097924745	34.0	34.0	34.0	34.0	34.0
410-419	32.873401046448	34.0	34.0	34.0	34.0	34.0
420-429	32.90318006988982	34.0	34.0	34.0	34.0	34.0
430-439	32.67378887477899	34.0	34.0	34.0	32.6	34.0
440-449	32.81119909693561	34.0	34.0	34.0	32.6	34.0
450-459	32.97970098374232	34.0	34.0	34.0	34.0	34.0
460-469	31.848985254250316	34.0	34.0	34.0	30.0	34.0
470-479	32.24583333333334	NaN	NaN	NaN	NaN	NaN
480-489	32.75	NaN	NaN	NaN	NaN	NaN
490-498	34.0	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.14738303194798874
1101	2	-0.10175456485597323
1101	3	0.009125782462859888
1101	4	-0.06830332547728091
1101	5	-0.09094081361890716
1101	6	-0.1239705222849139
1101	7	-0.150366928450822
1101	8	-0.05733475797961063
1101	9	0.05939515214712543
1101	10-19	-0.08229012197775631
1101	20-29	-0.06695290145511024
1101	30-39	-0.05059736394569114
1101	40-49	-0.15097969675396428
1101	50-59	-0.12809666369146555
1101	60-69	-0.10138317068359726
1101	70-79	-0.18473423963496316
1101	80-89	-0.1427393750380901
1101	90-99	-0.18374836704364128
1101	100-109	-0.2605206715476953
1101	110-119	-0.25743673828522873
1101	120-129	-0.20412664273268888
1101	130-139	-0.13770282121389954
1101	140-149	-0.5122076870370336
1101	150-159	-0.5212315630748279
1101	160-169	-0.39121048176443196
1101	170-179	-0.5163116701666297
1101	180-189	-0.5393733959801068
1101	190-199	-0.46782312878603705
1101	200-209	-0.536083042189702
1101	210-219	-0.45926569182211097
1101	220-229	-0.423629611711398
1101	230-239	-0.4104221295651982
1101	240-249	-0.38363170795805246
1101	250-259	-0.3678683102468625
1101	260-269	-0.332170670844647
1101	270-279	-0.3995733385159923
1101	280-289	-0.4257183331509822
1101	290-299	-0.43682049869275374
1101	300-309	-0.9446083762538393
1101	310-319	-1.420217316630918
1101	320-329	-1.3088845587298152
1101	330-339	-1.0816293924614833
1101	340-349	-0.9915435345298782
1101	350-359	-0.9392229915813672
1101	360-369	-0.8200669111891798
1101	370-379	-0.9440858500309446
1101	380-389	-0.5894490320092061
1101	390-399	-0.7127930045892654
1101	400-409	-0.7757892234969361
1101	410-419	-0.5242584529341769
1101	420-429	-0.48535291093073596
1101	430-439	-0.538639228099349
1101	440-449	-0.32397833310225366
1101	450-459	-0.7328490398877605
1101	460-469	-0.7210984742740827
1101	470-471	-1.096774193548387
1102	1	-0.2252334755474834
1102	2	-0.2744541846278352
1102	3	-0.01956369789201773
1102	4	-0.08229572091454429
1102	5	-0.015397087383163921
1102	6	-0.0809793942747774
1102	7	-0.09434157990836667
1102	8	-0.12444755899355187
1102	9	-0.05307126103411264
1102	10-19	-0.1130470294555721
1102	20-29	-0.062311583330910025
1102	30-39	-0.04899229423720186
1102	40-49	-0.02951759282493782
1102	50-59	-0.082787664958893
1102	60-69	-0.06888481833885862
1102	70-79	-0.16300394812673602
1102	80-89	-0.052184748929093416
1102	90-99	-0.09924342407785502
1102	100-109	-0.13416477801158067
1102	110-119	-0.10971658619396862
1102	120-129	-0.22353551472254907
1102	130-139	-0.2521428719109835
1102	140-149	-0.5409417808266355
1102	150-159	-0.5845953146591185
1102	160-169	-0.5095106085071421
1102	170-179	-0.6572115434239123
1102	180-189	-0.6660284023172522
1102	190-199	-0.5438773746668986
1102	200-209	-0.5063000257131449
1102	210-219	-0.5186563128613955
1102	220-229	-0.3762709298355986
1102	230-239	-0.5655704185385915
1102	240-249	-0.4805690970581864
1102	250-259	-0.3713152050504078
1102	260-269	-0.5248865136836827
1102	270-279	-0.5453675083512266
1102	280-289	-0.4808950124919136
1102	290-299	-0.5895449600362284
1102	300-309	-1.444308756481977
1102	310-319	-1.4766675067449846
1102	320-329	-1.3796678286917974
1102	330-339	-1.1382661478480465
1102	340-349	-1.0619451712479488
1102	350-359	-1.0319546328673184
1102	360-369	-0.8374266404616009
1102	370-379	-0.9738245979158897
1102	380-389	-0.6362880591787174
1102	390-399	-0.7333044827764681
1102	400-409	-0.608408791476613
1102	410-419	-0.4790384614051604
1102	420-429	-0.5020724722942234
1102	430-439	-0.5519034792441531
1102	440-449	-0.6426572267461523
1102	450-459	-0.338492930447849
1102	460-469	-0.22051204801739743
1102	470-471	-1.096774193548387
1103	1	-0.28515617406011273
1103	2	0.028475769781138638
1103	3	-0.24403545178311958
1103	4	0.14927164192819475
1103	5	-0.10500600266714599
1103	6	-0.08212784518799765
1103	7	-0.1964182105021024
1103	8	-0.17257508827078993
1103	9	0.014447737979374153
1103	10-19	-0.1696281489225555
1103	20-29	0.010125759996441275
1103	30-39	0.014599333142520265
1103	40-49	-0.029257662855656008
1103	50-59	0.06628803861188004
1103	60-69	0.0019331265784643392
1103	70-79	0.01320874167750219
1103	80-89	0.008980920485598176
1103	90-99	-0.11382676773898481
1103	100-109	-0.04813770761896308
1103	110-119	-0.05486359617309944
1103	120-129	0.021730636711033924
1103	130-139	-0.040527158458573354
1103	140-149	-0.2620124675672315
1103	150-159	-0.23681000722954337
1103	160-169	-0.30502586637981466
1103	170-179	-0.3780370069767045
1103	180-189	-0.47549560371587773
1103	190-199	-0.4254507689424898
1103	200-209	-0.3918287179828326
1103	210-219	-0.39459502689381765
1103	220-229	-0.27926020710469857
1103	230-239	-0.43737041292026646
1103	240-249	-0.28942588440308015
1103	250-259	-0.2635903441451646
1103	260-269	-0.2394349907055755
1103	270-279	-0.3164635601587591
1103	280-289	-0.2588906929945196
1103	290-299	-0.27787934267362857
1103	300-309	-0.7033012923772652
1103	310-319	-0.8915396982041486
1103	320-329	-0.7231531376085556
1103	330-339	-0.6437317827265723
1103	340-349	-0.596175086029227
1103	350-359	-0.5576794887565022
1103	360-369	-0.563237532658114
1103	370-379	-0.5023264410783099
1103	380-389	-0.31258797511829073
1103	390-399	-0.4222694188625411
1103	400-409	-0.2705265603115663
1103	410-419	-0.11713086372616743
1103	420-429	-0.3011727340543544
1103	430-439	-0.4036949178709719
1103	440-449	-0.20465039399372387
1103	450-459	-0.29502400844490495
1103	460-469	0.07355024699501911
1103	470-471	-1.096774193548387
1104	1	0.3055920373040877
1104	2	-0.11396139772762837
1104	3	-0.034634143668256456
1104	4	-0.2856440456988878
1104	5	0.23120877086862635
1104	6	0.04014766792745661
1104	7	0.004838981059798186
1104	8	0.02877419862242192
1104	9	0.18904242823207795
1104	10-19	0.06220812363627459
1104	20-29	-0.020264074123623743
1104	30-39	-0.021769293770262266
1104	40-49	-0.06711155270964042
1104	50-59	-0.013651973017417163
1104	60-69	-0.04340865544814676
1104	70-79	-0.08291115560911066
1104	80-89	-0.08347141566598282
1104	90-99	-0.1863829007463096
1104	100-109	-0.07921356166772853
1104	110-119	-0.168464993132865
1104	120-129	-0.17733920044274498
1104	130-139	-0.09479460330069145
1104	140-149	-0.5019088874063797
1104	150-159	-0.37549564433060567
1104	160-169	-0.481490814174407
1104	170-179	-0.5812608853097387
1104	180-189	-0.6604699795442883
1104	190-199	-0.49808130053119015
1104	200-209	-0.5138821188286613
1104	210-219	-0.4407360519329018
1104	220-229	-0.24343385917214988
1104	230-239	-0.520684364098905
1104	240-249	-0.3520335546801263
1104	250-259	-0.27642897506680697
1104	260-269	-0.2388018804475962
1104	270-279	-0.3169650282666865
1104	280-289	-0.35371168495153427
1104	290-299	-0.3522694368275623
1104	300-309	-1.2050785516924378
1104	310-319	-1.5972647773880766
1104	320-329	-1.2133222318600048
1104	330-339	-1.253569189322043
1104	340-349	-1.048438825388608
1104	350-359	-0.8414087718214311
1104	360-369	-0.8337951776908952
1104	370-379	-0.870507856503437
1104	380-389	-0.6155649743732141
1104	390-399	-0.5720318934781474
1104	400-409	-0.4498114457191704
1104	410-419	-0.4956640084897259
1104	420-429	-0.41203435285330414
1104	430-439	-0.3854854134404846
1104	440-449	-0.5763516325780245
1104	450-459	-0.40795418080797674
1104	460-469	0.4340166051364278
1104	470-471	-1.096774193548387
1201	1	-0.3797318057899517
1201	2	0.2635133733819899
1201	3	0.16105675430663524
1201	4	0.12619258732925687
1201	5	0.1973752626753722
1201	6	0.26820023157521433
1201	7	0.07291735856098569
1201	8	0.02162255355536047
1201	9	-0.0973932220581446
1201	10-19	0.052628315806074966
1201	20-29	-0.05046062170941923
1201	30-39	-0.04424168728812106
1201	40-49	0.003518395889095416
1201	50-59	-0.0750183712300796
1201	60-69	-0.08783112708687213
1201	70-79	-0.04026848123351101
1201	80-89	0.004198317968260312
1201	90-99	-0.060978521448717515
1201	100-109	-0.14498642994914945
1201	110-119	-0.14979750122800084
1201	120-129	-0.18634498968999935
1201	130-139	-0.18869174037829595
1201	140-149	-0.3824990585175101
1201	150-159	-0.46214527787955717
1201	160-169	-0.4311734245437151
1201	170-179	-0.4428148490948658
1201	180-189	-0.47659156128438696
1201	190-199	-0.43981331952173264
1201	200-209	-0.3899529786111344
1201	210-219	-0.45307386620902435
1201	220-229	-0.3804016371428176
1201	230-239	-0.47854928669507757
1201	240-249	-0.3343604999653209
1201	250-259	-0.2351947407645696
1201	260-269	-0.28836976257942837
1201	270-279	-0.2503766082707628
1201	280-289	-0.28485511789212126
1201	290-299	-0.218334031844428
1201	300-309	-1.0892245433746304
1201	310-319	-1.2944705409724264
1201	320-329	-1.0158164388388045
1201	330-339	-1.130473715803891
1201	340-349	-0.9933756871184336
1201	350-359	-0.8323550533433952
1201	360-369	-0.8767114161846372
1201	370-379	-0.7337694104305861
1201	380-389	-0.569187829823413
1201	390-399	-0.5679449171575612
1201	400-409	-0.5009225568302753
1201	410-419	-0.44388577533854345
1201	420-429	-0.4092474676074005
1201	430-439	-0.41106905773014546
1201	440-449	-0.37667634737175604
1201	450-459	-0.15887079819413685
1201	460-469	-0.33277952865035587
1201	470-471	-1.096774193548387
1202	1	-0.04210951530544094
1202	2	-0.028826441363449362
1202	3	-0.04762867966931594
1202	4	-0.010068882640808852
1202	5	0.23131099535359567
1202	6	0.06148051485931916
1202	7	-0.05715515806973315
1202	8	-0.028904463720081708
1202	9	0.025940255861549133
1202	10-19	-0.12247844807520636
1202	20-29	-8.969439056585315E-4
1202	30-39	0.01078459456853409
1202	40-49	0.0457184219146356
1202	50-59	-0.06123626812947691
1202	60-69	-0.025344386313122413
1202	70-79	0.03777246562311376
1202	80-89	-0.03301761912878476
1202	90-99	-0.04993225512853883
1202	100-109	0.01488868687005862
1202	110-119	-0.022801716577546927
1202	120-129	0.0021371295779673005
1202	130-139	0.019512615351466422
1202	140-149	-0.21661598419091632
1202	150-159	-0.1975258226020884
1202	160-169	-0.2276463717789028
1202	170-179	-0.265945051739223
1202	180-189	-0.21316770374662752
1202	190-199	-0.24158463385115425
1202	200-209	-0.22428776963784003
1202	210-219	-0.23608884667015673
1202	220-229	-0.14328132420536832
1202	230-239	-0.3632364083881612
1202	240-249	-0.17861733265656454
1202	250-259	-0.15448538694729308
1202	260-269	-0.1841386399715077
1202	270-279	-0.2342772458966067
1202	280-289	-0.32075972244185635
1202	290-299	-0.3522171219440793
1202	300-309	-1.0566514056797942
1202	310-319	-0.9390858163897207
1202	320-329	-0.8882072794244635
1202	330-339	-0.95084540789804
1202	340-349	-0.9111747935747374
1202	350-359	-0.7302665033999851
1202	360-369	-0.6754257148553648
1202	370-379	-0.8597186527323473
1202	380-389	-0.551291637768557
1202	390-399	-0.46569735241535426
1202	400-409	-0.39853125248244936
1202	410-419	-0.3940033804704086
1202	420-429	-0.5031937731431597
1202	430-439	-0.31466523749359965
1202	440-449	-0.519932638510781
1202	450-459	-0.1910800638733079
1202	460-469	-0.21547430441778914
1202	470-471	-1.096774193548387
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2404	3	-0.06547373792083278
2404	4	0.0853657392709195
2404	5	-0.05206311577718026
2404	6	0.2414011803529732
2404	7	0.13407431855157625
2404	8	0.11375397103717688
2404	9	0.02868052384976494
2404	10-19	0.05799381087596345
2404	20-29	0.019008729240333366
2404	30-39	-0.019664030612361216
2404	40-49	-0.015054516897841097
2404	50-59	0.0765230005771187
2404	60-69	0.09804992284158232
2404	70-79	0.07655017283506282
2404	80-89	0.040288922324016596
2404	90-99	0.08033604542638528
2404	100-109	0.19330282965135126
2404	110-119	0.16609707466441392
2404	120-129	0.1722263548692311
2404	130-139	0.1704516152369493
2404	140-149	0.42502588610445713
2404	150-159	0.4263415784119786
2404	160-169	0.41699959017801547
2404	170-179	0.5214101523753527
2404	180-189	0.4779110164899194
2404	190-199	0.4164444971132397
2404	200-209	0.45954381632348884
2404	210-219	0.4405961786814885
2404	220-229	0.3704183499192979
2404	230-239	0.5215299088041121
2404	240-249	0.34722968292923895
2404	250-259	0.3676741357962996
2404	260-269	0.30322884953904605
2404	270-279	0.3112784600451519
2404	280-289	0.33201979634542056
2404	290-299	0.3536380144967026
2404	300-309	1.281169081779744
2404	310-319	1.5386853212587681
2404	320-329	1.2447269520615478
2404	330-339	1.2506507034617833
2404	340-349	1.0905370410096964
2404	350-359	0.9498830036224746
2404	360-369	0.9027983166285622
2404	370-379	1.1044100732304472
2404	380-389	0.6900218585981932
2404	390-399	0.7261929704347594
2404	400-409	0.6932897390390806
2404	410-419	0.5291866959611156
2404	420-429	0.5667552725069243
2404	430-439	0.5339192815160416
2404	440-449	0.6162610318650579
2404	450-459	0.45445578750086213
2404	460-469	0.6591299339104708
2404	470-471	-1.096774193548387
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	44.0
19	106.0
20	181.0
21	314.0
22	408.0
23	507.0
24	655.0
25	977.0
26	1368.0
27	1666.0
28	2130.0
29	2753.0
30	4180.0
31	8298.0
32	29257.0
33	164646.0
34	3921.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0645840201971845	0.049228379031420355	0.0989083945677161	99.78727920620368
2	0.02258213121121519	0.10974915768650582	0.26421093517121774	99.60345777593106
3	0.005871274563380408	0.2136240668060718	99.70914609086023	0.07135856777031574
4	0.08355275340195198	99.59894678367063	0.14271713554063148	0.174783327386786
5	0.16168586874539895	0.032517828351029954	0.16439568777465147	99.64140061512892
6	99.78050465863055	0.05961601864355492	0.024840007768147883	0.1350393149577494
7	99.88934905630552	0.05826110912892867	0.039292375924161196	0.013097458641387064
8	99.85141158989599	0.06639056621668617	0.04245383145828911	0.039744012429036614
9	30.00492283790314	18.816983339129333	27.932362917029856	23.245730905937663
10-19	47.709931938378716	17.284490350786072	14.318006295812877	20.687571415022333
20-29	37.449473166017064	27.637941079501573	29.958855914405852	4.953729840075514
30-39	34.738254063599456	27.928072370233544	10.048912233478008	27.284761332689
40-49	29.823952090399565	39.49245089582101	10.328384902694914	20.355212111084516
50-59	30.255581098108998	29.83452038461365	7.700583062727794	32.20931545454956
60-69	37.91714276681555	12.301133155990733	12.706205937213493	37.07551813998022
70-79	39.89901407750986	30.292073327702933	10.471418183788959	19.33749441099825
80-89	39.526549451939104	0.7871121006968752	42.10950378697209	17.57683466039193
90-99	17.58948048252844	31.970219088868518	32.35812968290601	18.08217074569703
100-109	23.419204487460313	41.59368973475388	12.610865471034295	22.376240306751512
110-119	28.252663526287503	37.97400380277937	21.658770555106425	12.114562115826697
120-129	37.307433485233744	26.8762109503787	27.39812209541273	8.41823346897483
130-139	34.300618290375176	27.62028209216095	5.706156257197957	32.37294336026592
140-149	22.415893991879578	27.725106924942526	20.21972115962189	29.63927792355601
150-159	34.84082071385666	27.206086253539702	14.231698559731184	23.721394472872454
160-169	30.17595758229946	20.14294295379307	17.571821495187816	32.10927796871966
170-179	49.67820899027627	20.210598102223408	12.81979251818966	17.291400389310667
180-189	25.322220064403368	19.934061070288188	29.89743334974279	24.846285515565654
190-199	20.228076434962087	32.21048970946224	22.817940808519673	24.74349304705601
200-209	44.76083028210966	25.096391870609754	17.70945726532977	12.433320581950817
210-219	52.513808786136565	12.748117804865933	22.26974441890189	12.468328990095612
220-229	30.514412482944977	5.405940048515078	53.731058818322275	10.348588650217668
230-239	39.68376411928624	39.35903747228081	10.856664122447688	10.100534285985267
240-249	34.59684667392296	32.83225768572422	19.765329672066734	12.805565968286084
250-259	20.31937196893808	22.738079215665728	17.274873789494695	39.667675025901495
260-269	29.932796488074537	32.09622567372875	22.460696333163217	15.510281505033488
270-279	21.72208999308996	26.188910517259288	32.2762479845721	19.81275150507865
280-289	29.686067465461097	37.16530347714945	20.45963047101171	12.688998586377739
290-299	49.120844379609515	25.700330146285065	14.842130459720797	10.336695014384622
300-309	49.809590049544525	32.206199162665925	13.022261163325311	4.961949624464246
310-319	22.567463202915768	20.04755732396338	37.286838860611425	20.098140612509425
320-329	42.71032807023883	34.151326010767065	15.390215702568922	7.74813021642519
330-339	29.967301369368066	30.190094663438956	29.50518481049247	10.33741915670051
340-349	37.61594763850261	35.0874784228321	22.380276639390537	4.9162972992747545
350-359	25.12472144865668	29.883692676233686	19.60884029602544	25.382745579084194
360-369	45.25333550129626	24.701769599739844	7.691706638483149	22.353188260480746
370-379	25.030205114747822	17.704917144265046	29.68434469467202	27.580533046315107
380-389	24.795528319768138	37.123546699407434	17.830797821257466	20.25012715956696
390-399	39.92646925818064	15.502143516089935	12.586690302624676	31.98469692310475
400-409	45.281668166545316	27.35579684256701	16.92414834779699	10.438386643090684
410-419	28.206250650073034	44.58420198164872	4.9744266921118445	22.2351206761664
420-429	26.965179844142313	37.52030527557289	22.916968155539315	12.597546724745484
430-439	34.04473173626647	13.461567277187015	22.6620999859694	29.83160100057712
440-449	20.70044170002807	48.00625618601628	20.29438051201749	10.998921601938163
450-459	16.733504706446194	33.19214310475797	16.715508635272677	33.35884355352316
460-469	32.86214229607444	16.778637069015065	33.47776663750327	16.881453997407224
470-479	17.073170731707318	14.634146341463413	31.70731707317073	36.58536585365854
480-489	13.333333333333334	33.33333333333333	26.666666666666668	26.666666666666668
490-498	44.44444444444444	11.11111111111111	22.22222222222222	22.22222222222222
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.25
45	0.0
46	0.0
47	3.800000000000001
48	17.64999999999997
49	153.4999999999998
50	572.9999999999935
51	741.5500000000317
52	9050.449999997863
53	7666.049999995221
54	31122.40000007816
55	2470.4499999999443
56	153.44999999999982
57	58.800000000000296
58	21.799999999999955
59	11.79999999999999
60	6.200000000000003
61	4.200000000000001
62	1.0
63	1.5999999999999999
64	0.8
65	0.2
66	0.2
67	0.0
68	0.0
69	0.2
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013549095146262481
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.00140007316511379
210-219	0.0
220-229	5.871274563380408E-4
230-239	0.0
240-249	0.0
250-259	5.419638058504993E-4
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	4.5164119636139784E-5
350-359	0.0
360-369	0.0
370-379	4.516650632556921E-5
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-489	0.0
490-498	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
315-319	1.0
320-324	0.0
325-329	0.0
330-334	0.0
335-339	0.0
340-344	2.0
345-349	0.0
350-354	1.0
355-359	0.0
360-364	10.0
365-369	0.0
370-374	0.0
375-379	0.0
380-384	0.0
385-389	271.0
390-394	0.0
395-399	3.0
400-404	0.0
405-409	0.0
410-414	0.0
415-419	0.0
420-424	6.0
425-429	1.0
430-434	8.0
435-439	161451.0
440-444	6811.0
445-449	48.0
450-454	16.0
455-459	11.0
460-464	2706.0
465-469	50063.0
470-474	5.0
475-479	0.0
480-484	2.0
485-489	0.0
490-494	0.0
495-499	1.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	2.7969848746934516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.355078314225736	1.6042128653174776
2	12.25577264653641	0.6855842144008816
3	5.473922170192153	0.45931432545829814
4	3.8591958663006616	0.4317644986608977
5	2.7934765057322783	0.39066557671723495
6	1.7761989342806392	0.2980800932177746
7	1.4855481995801711	0.290853909139768
8	1.340222832229937	0.29988663923727626
9	1.2433392539964476	0.3129840978786633
>10	10.124333925399645	5.552419190938365
>50	1.4371064104634264	2.735562310030395
>100	0.3875343129339577	2.8164052444030947
>500	0.12917810431131924	2.1768879534995054
>1k	0.16147263038914905	12.695502152047947
>5k	0.06458905215565962	11.355948278587462
>10k+	0.11303084127240433	57.893928650464964
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	25467	11.50182686966222	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	21516	9.717411038899453	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	19273	8.704390358463893	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	16844	7.607365288121508	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	16446	7.427613959181093	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	14390	6.499049305157237	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	14251	6.436271830979555	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	7806	3.525474557057498	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	6376	2.8796343550856527	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	5890	2.6601390137162007	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	5072	2.2907003527281105	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	4983	2.2505047037941983	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4707	2.1258530284485837	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	4371	1.9741031628104435	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	4338	1.959199158149555	No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2151	0.97147012198702	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2006	0.9059828287800846	No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1605	0.7248765903250428	No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1504	0.6792613033326258	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	1424	0.6431303829425925	No Hit
CCTACGGGGGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1021	0.46112087147779984	No Hit
CCTACGGGAGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	964	0.4353775906999011	No Hit
CCTACGGGGGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	636	0.28724081710076466	No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	565	0.2551746252546101	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	563	0.25427135224485925	No Hit
CCTACGGGCGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	547	0.24704516816685257	No Hit
CCTACGGGTGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	536	0.242077166613223	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	506	0.22852807146696053	No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	503	0.22717316195233428	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	453	0.20459133670856347	No Hit
CCTACGGGCGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	429	0.19375206059155348	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	370	0.16710550680390396	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	363	0.16394405126977604	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	360	0.1625891417551498	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	333	0.15039495612351356	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	320	0.14452368156013315	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	316	0.14271713554063148	No Hit
CCTACGGGCGGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCA	303	0.13684586097725107	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	292	0.13187785942362149	No Hit
CCTACGGGGGGCAGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	289	0.13052294990899524	No Hit
CCTACGGGAGGCAGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	267	0.1205869468017361	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	263	0.11878040078223444	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	261	0.11787712777248359	No Hit
CCTTCGGGTTGTAAAATTCTGTTATAAGGGAAGAACGACTTTAGTAGGAA	243	0.1097476706847261	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	233	0.10523130563597194	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0
320-329	0.0	4.5163650487541606E-5	0.0	0.0	0.0
330-339	0.0	9.032730097508319E-4	0.0	0.0	0.0
340-349	0.0	9.032730097508319E-4	0.0	0.0	0.0
350-359	0.0	9.032730097508319E-4	0.0	0.0	0.0
360-369	0.0	9.032730097508319E-4	0.0	0.0	0.0
370-379	0.0	9.032730097508319E-4	0.0	0.0	0.0
380-389	0.0	9.032730097508319E-4	0.0	0.0	0.0
390-399	0.0	9.032730097508319E-4	0.0	0.0	0.0
400-409	0.0	9.032730097508319E-4	0.0	0.0	0.0
410-419	0.0	9.032730097508319E-4	0.0	0.0	0.0
420-429	0.0	9.032730097508319E-4	0.0	0.0	0.0
430-439	0.0	9.032730097508319E-4	0.0	0.0	0.0
440-449	0.0	9.032730097508319E-4	0.0	0.0	0.0
450-459	0.0	9.032730097508319E-4	0.0	0.0	0.0
460-469	0.0	9.032730097508319E-4	0.0	1.8065460195016642E-4	0.0
470-479	0.0	9.032730097508319E-4	0.0	4.5163650487541594E-4	0.0
480-487	0.0	9.032730097508319E-4	0.0	4.5163650487541594E-4	0.0
>>END_MODULE
