FastQCFastQC Report
Tue 15 Oct 2024
IPMS2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIPMS2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236364
Sequences flagged as poor quality0
Sequence length316-484
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT125985.329914877054035No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT108104.573454502377689No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT98414.16349359462524No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT86283.650302076458344No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT84273.565263745748083No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT82323.482763872670965No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT73313.1015721514274595No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT72653.0736491174628964No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT70772.9941107782911103No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT70302.974226193498164No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT66642.8193802778764954No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT60792.5718806586451404No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT56912.4077270650352847No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT56592.3941886243251935No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT54962.325227191958166No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT50252.125958267756511No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT48552.0540353014841513No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT47101.9926892420165507No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT46221.9554585300638No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT41571.7587280634952869No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT39241.6601512920749355No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC21920.9273831886412484No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT21910.926960112369058No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC17710.7492680780491108No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC17590.7441911627828265No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT15700.6642297473388502No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC14910.6308067218358125No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC14210.6011913827824881No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC14120.5973836963327749No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC13410.56734528100726No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC12850.5436530097646003No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT12680.5364607131373644No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC12340.5220761198828925No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC11520.4873838655632837No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC10360.43830701798920313No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC10000.4230762721903505No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9630.40742245011930756No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC9400.39769169585892944No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC8660.3663840517168435No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC8620.3646917466280821No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8260.3494610008292295No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC8180.3460763906517067No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7480.31646105159838217No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC7370.3118072126042883No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC6830.2889610939060094No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC6730.2847303311841059No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT6490.27457650065153744No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC6460.2733072718349664No Hit
CCTACGGGGGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT6340.2682303565686822No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6230.26357651757458833No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC5770.2441150090538322No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC5720.24199962769288047No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT5720.24199962769288047No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT5590.23649963615440592No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC5400.22846118698278928No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5400.22846118698278928No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC5070.2144996700005077No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5060.21407659372831736No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC5050.21365351745612696No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT4880.20646122082889104No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC4700.19884584792946472No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC4680.19799969538508402No Hit
CCTACGGGCGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC4590.19419200893537086No Hit
CCTACGGGAGGCAGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4590.19419200893537086No Hit
CCTACGGGGGGCAGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4560.19292278011879982No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT4530.19165355130222878No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4250.17980741568089897No Hit
CCTACGGGCGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT4220.1785381868643279No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT4100.1734612715980437No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT4040.1709228139649016No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT3980.16838435633175947No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC3840.1624612885210946No Hit
CCTACGGGGGGCAGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC3830.16203821224890425No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3790.16034590716014283No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT3480.14723054272224198No Hit
CCTACGGGGGGCAGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT3210.1358074833731025No Hit
CCTACGGGGGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2940.12438442402396303No Hit
CCTACGGGAGGCAGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2920.12353827147958234No Hit
CCTACGGGGGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC2800.11846135621329813No Hit
CCTACGGGTGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC2670.11296136467482357No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC2670.11296136467482357No Hit
CCTACGGGTGGCTGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC2640.11169213585825254No Hit
CCTACGGGCGGCAGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2640.11169213585825254No Hit
CCTACGGGAGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2560.10830752568072972No Hit
CCTACGGGAGGCAGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC2440.10323061041444552No Hit
CCTACGGGTGGCAGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2370.10026907650911307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)