##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	ICMS2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	121148
Sequences flagged as poor quality	0
Sequence length	362-482
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.347252946808865	34.0	34.0	34.0	20.0	34.0
2	33.20096906263414	34.0	34.0	34.0	34.0	34.0
3	33.66163700597616	34.0	34.0	34.0	34.0	34.0
4	33.48221184006339	34.0	34.0	34.0	34.0	34.0
5	33.38594941724172	34.0	34.0	34.0	34.0	34.0
6	33.228513883844556	34.0	34.0	34.0	34.0	34.0
7	33.58574635982435	34.0	34.0	34.0	34.0	34.0
8	33.54177534915971	34.0	34.0	34.0	34.0	34.0
9	33.51574933139631	34.0	34.0	34.0	34.0	34.0
10-19	33.36903622016045	34.0	34.0	34.0	32.6	34.0
20-29	33.60897827450721	34.0	34.0	34.0	34.0	34.0
30-39	33.56230560966752	34.0	34.0	34.0	34.0	34.0
40-49	33.64831445834846	34.0	34.0	34.0	34.0	34.0
50-59	33.573880707894475	34.0	34.0	34.0	34.0	34.0
60-69	33.45558572985108	34.0	34.0	34.0	34.0	34.0
70-79	33.49782167266484	34.0	34.0	34.0	34.0	34.0
80-89	33.62335242843464	34.0	34.0	34.0	34.0	34.0
90-99	33.4875590187209	34.0	34.0	34.0	34.0	34.0
100-109	33.39396358173474	34.0	34.0	34.0	34.0	34.0
110-119	33.44447865420807	34.0	34.0	34.0	34.0	34.0
120-129	33.26467874005349	34.0	34.0	34.0	34.0	34.0
130-139	33.40011473569519	34.0	34.0	34.0	34.0	34.0
140-149	33.12764717535576	34.0	34.0	34.0	34.0	34.0
150-159	33.070451018588834	34.0	34.0	34.0	34.0	34.0
160-169	33.20241852940206	34.0	34.0	34.0	34.0	34.0
170-179	33.03281523425891	34.0	34.0	34.0	33.1	34.0
180-189	33.05009492521544	34.0	34.0	34.0	33.1	34.0
190-199	33.21726565853337	34.0	34.0	34.0	34.0	34.0
200-209	33.15559150790769	34.0	34.0	34.0	33.1	34.0
210-219	33.23754003367782	34.0	34.0	34.0	34.0	34.0
220-229	33.39199574074685	34.0	34.0	34.0	34.0	34.0
230-239	33.37083649750718	34.0	34.0	34.0	34.0	34.0
240-249	33.37075312840492	34.0	34.0	34.0	34.0	34.0
250-259	33.40386304355003	34.0	34.0	34.0	34.0	34.0
260-269	33.42369003202693	34.0	34.0	34.0	34.0	34.0
270-279	33.391762967609864	34.0	34.0	34.0	34.0	34.0
280-289	33.314501271172446	34.0	34.0	34.0	34.0	34.0
290-299	33.25093769604121	34.0	34.0	34.0	34.0	34.0
300-309	31.311555287747222	34.0	34.0	34.0	22.8	34.0
310-319	30.612246178228286	34.0	31.2	34.0	17.8	34.0
320-329	31.88895813385281	34.0	34.0	34.0	20.0	34.0
330-339	31.671827021494373	34.0	34.0	34.0	21.4	34.0
340-349	32.12691583847855	34.0	34.0	34.0	22.8	34.0
350-359	32.36769323472117	34.0	34.0	34.0	29.8	34.0
360-369	32.23522112079472	34.0	34.0	34.0	28.4	34.0
370-379	31.97879053027802	34.0	34.0	34.0	27.0	34.0
380-389	32.5711716121238	34.0	34.0	34.0	34.0	34.0
390-399	32.59303615651312	34.0	34.0	34.0	34.0	34.0
400-409	32.586705025756174	34.0	34.0	34.0	32.6	34.0
410-419	32.73456997622405	34.0	34.0	34.0	34.0	34.0
420-429	32.74186344891754	34.0	34.0	34.0	34.0	34.0
430-439	32.58418136475457	34.0	34.0	34.0	32.6	34.0
440-449	32.61610111751576	34.0	34.0	34.0	32.6	34.0
450-459	32.83529595551358	34.0	34.0	34.0	34.0	34.0
460-469	32.41823808044009	34.0	34.0	34.0	28.0	34.0
470-479	31.269166666666667	NaN	NaN	NaN	NaN	NaN
480-482	29.833333333333332	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.20885299040840266
1101	2	-0.055947238910789565
1101	3	-0.12536698615953412
1101	4	-0.11502589972600674
1101	5	0.03287040983990153
1101	6	0.0940352554202093
1101	7	-0.049828759790443655
1101	8	-0.14223280083343326
1101	9	-0.056296512258846576
1101	10-19	-0.002164978988759003
1101	20-29	-0.014638659923853936
1101	30-39	0.03813095841487524
1101	40-49	-0.10272212851284479
1101	50-59	-0.07716813011288792
1101	60-69	-0.06390021198738083
1101	70-79	-0.16677914515666714
1101	80-89	-0.039508274561512735
1101	90-99	-0.13594852878688357
1101	100-109	-0.07611821376790573
1101	110-119	-0.0892420494426247
1101	120-129	-0.13705130400436616
1101	130-139	-0.023944535780017873
1101	140-149	-0.3043166625334166
1101	150-159	-0.3446666059363679
1101	160-169	-0.2935662043317109
1101	170-179	-0.415434517868313
1101	180-189	-0.4196109393073968
1101	190-199	-0.2273321254127083
1101	200-209	-0.2191580857508626
1101	210-219	-0.33961715854899666
1101	220-229	-0.23284602479374428
1101	230-239	-0.34039876820939696
1101	240-249	-0.23075496963633668
1101	250-259	-0.2138809078692816
1101	260-269	-0.23622936961720598
1101	270-279	-0.258326655675468
1101	280-289	-0.29256331603569663
1101	290-299	-0.3474554858045025
1101	300-309	-1.0931408940066305
1101	310-319	-1.3611084777890738
1101	320-329	-1.0238489621735667
1101	330-339	-0.943063754125788
1101	340-349	-0.7970655901450989
1101	350-359	-0.7455075159364064
1101	360-369	-0.7386075349325907
1101	370-379	-0.7406143547631636
1101	380-389	-0.533117007618209
1101	390-399	-0.6800605156310517
1101	400-409	-0.45222005107346064
1101	410-419	-0.3974367203315765
1101	420-429	-0.4326394488147187
1101	430-439	-0.4081458841571006
1101	440-449	-0.333024945847022
1101	450-459	-0.02734405583038324
1101	460-469	-0.062437385095840625
1101	470-479	-3.0
1102	1	0.45962040441779095
1102	2	0.0038490534907325014
1102	3	0.09580898956380679
1102	4	0.2505300898182057
1102	5	0.22529296790257547
1102	6	0.16740003623924338
1102	7	-0.03905449064403399
1102	8	0.22424858606077436
1102	9	-0.04014412214978336
1102	10-19	-0.08223588605844157
1102	20-29	0.012453248858406596
1102	30-39	0.012293744221445024
1102	40-49	-0.009095352080422003
1102	50-59	0.004716916307209829
1102	60-69	-0.028090939986668673
1102	70-79	-0.06418683076193332
1102	80-89	-0.08378613412946123
1102	90-99	-0.08692350099491364
1102	100-109	-0.048517937350510465
1102	110-119	-0.17612183792324032
1102	120-129	-0.15572029417952393
1102	130-139	-0.06712934484169608
1102	140-149	-0.47406338007690607
1102	150-159	-0.466644792998828
1102	160-169	-0.5288400678656373
1102	170-179	-0.4988656989517537
1102	180-189	-0.6068662948943384
1102	190-199	-0.39774885467388543
1102	200-209	-0.4140239331804807
1102	210-219	-0.4107820174859853
1102	220-229	-0.3014816646098808
1102	230-239	-0.290225406433116
1102	240-249	-0.2566246328277586
1102	250-259	-0.33849407374975726
1102	260-269	-0.395833371660288
1102	270-279	-0.3753311324353845
1102	280-289	-0.42120196038867164
1102	290-299	-0.5686801350248203
1102	300-309	-1.3740314387291548
1102	310-319	-1.5026425943050121
1102	320-329	-1.1729124480372235
1102	330-339	-1.1370558822392383
1102	340-349	-0.8690891457140069
1102	350-359	-0.5669581063279026
1102	360-369	-0.6726098784713272
1102	370-379	-1.0737435798931152
1102	380-389	-0.5411829872474456
1102	390-399	-0.4455594189751082
1102	400-409	-0.42217888891860156
1102	410-419	-0.2655856251779056
1102	420-429	-0.32479400219030197
1102	430-439	-0.3330779126574228
1102	440-449	-0.40298695445790145
1102	450-459	-0.358959635132976
1102	460-469	-0.17677415432151378
1102	470-479	-3.0
1103	1	-0.414803206366571
1103	2	-0.16592109660572163
1103	3	-0.24787778406814454
1103	4	0.05726325690049805
1103	5	-0.11882315687980594
1103	6	-0.4173821121633381
1103	7	-0.2566825902064451
1103	8	-0.16154400792595425
1103	9	-0.30196598259301055
1103	10-19	-0.12797766368982622
1103	20-29	0.004587300521691873
1103	30-39	1.5477623790616235E-5
1103	40-49	-0.05369905508706552
1103	50-59	-0.032967175350435696
1103	60-69	-0.09072562685439323
1103	70-79	-0.014076258136888953
1103	80-89	-0.08315739936259803
1103	90-99	-0.10172200003034249
1103	100-109	0.0285129524062242
1103	110-119	0.011406393385293256
1103	120-129	0.12685981215839348
1103	130-139	0.14139474587489786
1103	140-149	-0.17998783780052463
1103	150-159	-0.12080891100569602
1103	160-169	-0.2244618624358239
1103	170-179	-0.3065222650835935
1103	180-189	-0.42926313298777075
1103	190-199	-0.37067208904079507
1103	200-209	-0.28494803801274315
1103	210-219	-0.2565420278374688
1103	220-229	-0.09761926871281901
1103	230-239	-0.2230260698688653
1103	240-249	-0.18674155749721422
1103	250-259	-0.1519151201902602
1103	260-269	-0.1287646048979525
1103	270-279	-0.14687632605510004
1103	280-289	-0.2136453801411804
1103	290-299	-0.36000606548169856
1103	300-309	-0.8838865180120798
1103	310-319	-0.9563920649679858
1103	320-329	-0.6192717855425158
1103	330-339	-0.6072959659921189
1103	340-349	-0.5625418347461455
1103	350-359	-0.48265118495210046
1103	360-369	-0.47661049014968526
1103	370-379	-0.5211798241880459
1103	380-389	-0.24420020834563871
1103	390-399	-0.33521793777245534
1103	400-409	-0.2136618560291268
1103	410-419	-0.4148980751849578
1103	420-429	-0.42265195165627034
1103	430-439	-0.22764592339729006
1103	440-449	-0.386710295238089
1103	450-459	-0.523479645264679
1103	460-469	0.46498671400391345
1103	470-479	31.0
1104	1	0.09848004543869138
1104	2	0.4396053417823538
1104	3	-0.14883865059505297
1104	4	0.006645972949883117
1104	5	0.10617101968669118
1104	6	0.3931874196326106
1104	7	-0.046794400129577696
1104	8	0.3307108255238447
1104	9	-0.04240390479193934
1104	10-19	-0.04538972984403955
1104	20-29	0.008401649461831084
1104	30-39	-0.10814897613814622
1104	40-49	-0.04504252119467367
1104	50-59	-0.02617006377345632
1104	60-69	0.006398761685083798
1104	70-79	-0.01919622086690964
1104	80-89	-0.02771889482031753
1104	90-99	-0.16967156909625913
1104	100-109	-0.15127216289775447
1104	110-119	-0.18412528609292877
1104	120-129	-0.25575723885487633
1104	130-139	-0.19117047658024688
1104	140-149	-0.6954426479565896
1104	150-159	-0.4298398916718966
1104	160-169	-0.3619344922983885
1104	170-179	-0.5765560409978647
1104	180-189	-0.6614970020769988
1104	190-199	-0.6471506588055291
1104	200-209	-0.5050155898412996
1104	210-219	-0.5070900129294813
1104	220-229	-0.3769781086848738
1104	230-239	-0.4965195476233575
1104	240-249	-0.45575273848952946
1104	250-259	-0.39830750313925023
1104	260-269	-0.44035847864591915
1104	270-279	-0.46629631207886035
1104	280-289	-0.5289726571036795
1104	290-299	-0.5841593382513253
1104	300-309	-1.528268069374768
1104	310-319	-1.5465673503162236
1104	320-329	-1.412904443357565
1104	330-339	-1.3836825255076093
1104	340-349	-1.17086002715239
1104	350-359	-0.9121771574654929
1104	360-369	-0.8472405856539886
1104	370-379	-1.0146616550755247
1104	380-389	-0.4989626122589996
1104	390-399	-0.5993286994775566
1104	400-409	-0.6396512573801623
1104	410-419	-0.2887025242881549
1104	420-429	-0.6154506937946351
1104	430-439	-0.28379263805557287
1104	440-449	-0.4959140683590064
1104	450-459	-0.5475465529509407
1104	460-469	-0.8200815049495667
1104	470-479	-3.0
1201	1	-0.4910390774137632
1201	2	0.23081659966027246
1201	3	-0.052386954143358366
1201	4	0.00947770911494672
1201	5	0.13738918423819513
1201	6	0.36345418989937883
1201	7	0.24546149212631718
1201	8	-0.023256195109837563
1201	9	-0.06440166012303195
1201	10-19	0.02279640500876212
1201	20-29	0.06842927615612382
1201	30-39	0.01612798147213823
1201	40-49	0.10649853034637857
1201	50-59	0.06493932066926789
1201	60-69	-0.03560072031439887
1201	70-79	0.030797735793704817
1201	80-89	0.06924562214419439
1201	90-99	-0.05030525639661931
1201	100-109	-0.02476089638648915
1201	110-119	-0.03880196743628517
1201	120-129	0.04147145837382027
1201	130-139	0.02108368900724855
1201	140-149	-0.13772357690419312
1201	150-159	-0.21573128422996746
1201	160-169	-0.20045473748530895
1201	170-179	-0.3595932507017423
1201	180-189	-0.4100785439918724
1201	190-199	-0.253585951907489
1201	200-209	-0.3430713612304004
1201	210-219	-0.3451250642978678
1201	220-229	-0.20272273442949285
1201	230-239	-0.21270533714247364
1201	240-249	-0.2886250513618407
1201	250-259	-0.24728445211619743
1201	260-269	-0.22853425348835543
1201	270-279	-0.16807305385560767
1201	280-289	-0.20775190254958886
1201	290-299	-0.18682358758223927
1201	300-309	-1.3175299186366125
1201	310-319	-1.3712362616518021
1201	320-329	-1.2971019742217607
1201	330-339	-1.1216398767982945
1201	340-349	-1.068670611629642
1201	350-359	-0.9357133743350339
1201	360-369	-1.0774538292005715
1201	370-379	-1.0021882026020812
1201	380-389	-0.6607756140720014
1201	390-399	-0.6486734288222848
1201	400-409	-0.5968506012461781
1201	410-419	-0.5097264386453944
1201	420-429	-0.42155791990185776
1201	430-439	-0.41955036189542483
1201	440-449	-0.29771310633393
1201	450-459	-0.40731412155562907
1201	460-469	0.3021015801110636
1201	470-479	24.0
1202	1	0.21384120937361217
1202	2	-0.14783164695862894
1202	3	0.11985645037388792
1202	4	-0.07109860129020973
1202	5	-0.01795587819733413
1202	6	0.13778580775178284
1202	7	-0.007928150016383029
1202	8	-0.13703646536932723
1202	9	0.13386462127283494
1202	10-19	-0.02809068670962489
1202	20-29	-0.018064588577338725
1202	30-39	0.06545119289804546
1202	40-49	0.05296526654415601
1202	50-59	0.03230015869024783
1202	60-69	0.03507937383758275
1202	70-79	-0.030342346617764804
1202	80-89	0.014048740785938207
1202	90-99	-0.06767150872619965
1202	100-109	-0.05152752828760043
1202	110-119	0.002893338073207019
1202	120-129	0.12562463201354745
1202	130-139	0.1211999541601827
1202	140-149	-0.129362174655256
1202	150-159	-0.22553973169122798
1202	160-169	-0.13863587175445247
1202	170-179	-0.2805996281873391
1202	180-189	-0.18376329444923556
1202	190-199	-0.1251705205580791
1202	200-209	-0.12625679845007198
1202	210-219	-0.04699100601711592
1202	220-229	0.022351783554562132
1202	230-239	-0.011737030795195835
1202	240-249	-0.06736733597209366
1202	250-259	0.04451600496543762
1202	260-269	-0.04978285004257543
1202	270-279	-0.07528662268286723
1202	280-289	-0.07057668982425014
1202	290-299	-0.09224530522889296
1202	300-309	-0.7679964040664231
1202	310-319	-0.5534471233247586
1202	320-329	-0.5812095536838626
1202	330-339	-0.5800084031374837
1202	340-349	-0.4007136297362237
1202	350-359	-0.4149469362091942
1202	360-369	-0.4042746041876022
1202	370-379	-0.32299453563335234
1202	380-389	-0.41013530084000394
1202	390-399	-0.17331765175501346
1202	400-409	-0.2773197653339281
1202	410-419	-0.34681958185100115
1202	420-429	-0.5732828046340828
1202	430-439	-0.44806913360528
1202	440-449	-0.5244673298191103
1202	450-459	-0.3719590298060993
1202	460-469	-0.13190956320159586
1202	470-479	-3.0
1203	1	0.4011923058662106
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2404	50-59	0.023483481569449793
2404	60-69	0.08951491448709703
2404	70-79	0.13155744728640428
2404	80-89	0.05899043230784429
2404	90-99	0.1553843419631349
2404	100-109	0.120915155310513
2404	110-119	0.17818872075859105
2404	120-129	0.14970136110634513
2404	130-139	0.036140621603450995
2404	140-149	0.24492449056062782
2404	150-159	0.3235949218501659
2404	160-169	0.29063741643408036
2404	170-179	0.4709819332765832
2404	180-189	0.47761229919635184
2404	190-199	0.2823865398241594
2404	200-209	0.43367292415261716
2404	210-219	0.3004679988344705
2404	220-229	0.2387965665138978
2404	230-239	0.2701367771132581
2404	240-249	0.2617593809597665
2404	250-259	0.23314489166393315
2404	260-269	0.23199431044334773
2404	270-279	0.24402558049433765
2404	280-289	0.20798358360475078
2404	290-299	0.31208718750654896
2404	300-309	1.3076772476700214
2404	310-319	1.4397664354765567
2404	320-329	1.1850969786578176
2404	330-339	1.3016823507124151
2404	340-349	1.0369494227862077
2404	350-359	0.8362883586614487
2404	360-369	0.9825493438916055
2404	370-379	1.0242698942748696
2404	380-389	0.7022163677681519
2404	390-399	0.6498297259635919
2404	400-409	0.6854453750288556
2404	410-419	0.5389854079722127
2404	420-429	0.6123154493118292
2404	430-439	0.5193242064173873
2404	440-449	0.8799600089643249
2404	450-459	0.5784750338565843
2404	460-469	0.32186104298564544
2404	470-479	-3.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	9.0
18	23.0
19	46.0
20	99.0
21	169.0
22	222.0
23	288.0
24	403.0
25	541.0
26	746.0
27	928.0
28	1185.0
29	1628.0
30	2405.0
31	4955.0
32	18455.0
33	88053.0
34	993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.061082312543335425	0.06025687588734441	0.09740152540694028	99.78125928616238
2	0.029716210192660096	0.10565763624056923	0.312020207022931	99.55260594654384
3	0.014032423151847329	0.2525836167332519	99.6500148578598	0.08336910225509295
4	0.0817182289431109	99.70366824049923	0.018985043087793443	0.19562848746987158
5	0.18242150097401527	0.02971571961567669	0.1840723742859973	99.60379040512431
6	99.76805229966652	0.06603493247928155	0.04044639614355994	0.1254663717106349
7	99.88196255819328	0.058606002575362366	0.052002509327434214	0.007428929903919173
8	99.80519694918611	0.07676560900716478	0.07428929903919174	0.04374814276752402
9	30.438802126324827	19.804701687192523	27.0404794136098	22.716016772872848
10-19	49.756826361145045	15.553867996169974	14.232508997259549	20.456796645425428
20-29	39.550219566150496	29.641017598309503	29.966982533760362	0.8417803017796415
30-39	31.17055172186086	30.098887311387724	10.104087562320467	28.626473404430946
40-49	28.976788721233532	36.87250305411563	10.437646515006438	23.7130617096444
50-59	30.445323075907154	27.157361244098126	12.425875788292007	29.971439891702712
60-69	37.51931521775019	10.183329481295605	16.545465051011984	35.75189024994222
70-79	39.951711955624525	33.88409218476574	8.70695347839007	17.457242381219665
80-89	38.55069831941097	1.6014296562881762	43.40641199194374	16.441460032357117
90-99	17.07151583187506	32.46632218443556	32.79608412850398	17.666077855185396
100-109	33.47211674976062	33.95441938785618	8.22712715026249	24.34633671212071
110-119	37.221497672268626	40.37227193185195	15.200250932743423	7.205979463135999
120-129	30.577145309868918	28.29621619836894	28.869234324957905	12.257404166804239
130-139	24.989682041800112	25.693284247366854	14.800904678574966	34.516129032258064
140-149	20.353204345098558	27.295456796645425	22.848581899825007	29.502756958431007
150-159	35.50401162214812	28.89465777396243	7.429507709578368	28.171822894311088
160-169	30.889407996830325	20.156007527982304	16.36494205434675	32.589642420840626
170-179	46.027751180374416	17.560174332221745	14.85026579060323	21.561808696800608
180-189	27.946148512563145	19.97886882160663	26.152144484432267	25.92283818139796
190-199	22.848086637831415	33.4100439132301	21.53135008419454	22.21051936474395
200-209	43.09949606052226	25.976945078438735	17.30910351601999	13.614455345019005
210-219	49.523805593158784	17.331693465843433	22.656007527982304	10.488493413015485
220-229	32.9127444538728	7.190484995162925	46.829322248232735	13.067448302731544
230-239	35.63608148710668	38.59931653844884	13.823752765212799	11.940849209231684
240-249	34.03085482220094	32.02776768910754	18.590731997226534	15.350645491464984
250-259	20.39835737427516	21.631317195980106	22.6613013062589	35.309024123485834
260-269	29.879486248225312	35.82857331528378	20.401409845808434	13.89053059068247
270-279	21.129114801730115	24.196932677386336	31.14157889523558	23.532373625647967
280-289	28.160762043120812	35.766005216759666	20.091375837818205	15.981856902301317
290-299	41.21042031234523	27.08125598441576	15.965430712847095	15.742892990391919
300-309	46.87275068511242	32.54960874302506	14.430943969359792	6.1466966025027245
310-319	23.081355036814475	18.441823224485752	38.229520916564866	20.24730082213491
320-329	46.7557863109585	25.9561858223	17.573051144055203	9.714976722686302
330-339	29.95055634430614	31.005546934328258	28.546488592465412	10.497408128900188
340-349	38.96374682206887	34.56342655264635	20.453990160795062	6.018836464489715
350-359	26.273648760194142	30.632036847492323	18.80163106283224	24.292683329481296
360-369	44.84491788799419	23.241061368354583	9.409676793607687	22.50434395004354
370-379	26.131506523652053	20.781748632616775	29.265550527554858	23.821194316176314
380-389	28.511829781894843	33.225664790653056	17.953846433235324	20.30865899421677
390-399	39.89301634472511	14.322436849925706	8.184249628528976	37.600297176820206
400-409	46.00729758288205	32.003120459648656	11.372424382512218	10.617157574957073
410-419	28.553184358187565	40.01137580355027	6.132862451015113	25.302577387247048
420-429	29.27852549840627	36.99091315112304	23.975841051554887	9.754720298915803
430-439	35.61978918594297	14.143428109921514	21.16879620617532	29.067986497960195
440-449	20.04821885712941	45.50380076512134	21.49033923168847	12.957641146060775
450-459	16.739486619333697	33.151564972101326	16.75694088633894	33.35200752222604
460-469	32.76283734465794	16.873631425781806	33.51359805863896	16.849933170921293
470-479	22.448979591836736	14.285714285714285	34.69387755102041	28.57142857142857
480-482	33.33333333333333	0.0	33.33333333333333	33.33333333333333
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.2
40	0.4
41	0.2
42	0.0
43	0.0
44	0.0
45	0.0
46	0.2
47	1.0
48	12.599999999999987
49	180.3999999999983
50	2099.2000000002818
51	656.2500000000123
52	224.19999999999584
53	2100.7000000002804
54	14143.45000001639
55	7056.049999995774
56	1743.2000000002593
57	86.00000000000068
58	22.199999999999953
59	7.0000000000000036
60	4.200000000000001
61	2.8000000000000003
62	2.1999999999999997
63	1.2
64	1.2
65	0.4
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0016508733119820387
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0020635916399775484
210-219	0.0
220-229	3.301746623964077E-4
230-239	0.0
240-249	0.0
250-259	4.127183279955097E-4
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	1.6509046544780376E-4
370-379	1.650927821435647E-4
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-482	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
360-364	3.0
365-369	1.0
370-374	0.0
375-379	0.0
380-384	0.0
385-389	4.0
390-394	0.0
395-399	4.0
400-404	0.0
405-409	0.0
410-414	2.0
415-419	0.0
420-424	4.0
425-429	0.0
430-434	6.0
435-439	71777.0
440-444	13737.0
445-449	41.0
450-454	53.0
455-459	9.0
460-464	1998.0
465-469	33501.0
470-474	3.0
475-479	3.0
480-483	2.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.320335457456995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.113870844478406	2.4243074586456235
2	13.851738632021398	1.1968831511869777
3	7.260221627818113	0.9409977878297618
4	4.088651127244937	0.7065737775283125
5	3.1142529614061902	0.6727308746326808
6	2.1971723347344287	0.5695512926338033
7	1.7768437141765379	0.5373592630501536
8	1.4138326327856323	0.4886585003466834
9	1.0699273977837218	0.4160200746194737
>10	7.814291173098968	6.1684881302208865
>50	0.3439052350019106	1.1754217981312114
>100	0.45854031333588074	4.2064251989302335
>500	0.05731753916698509	1.3867335820649123
>1k	0.2865876958349255	27.764387360913922
>5k	0.13374092472296523	42.08736421567009
>10k+	0.019105846388995033	9.258097533595272
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	11216	9.258097533595272	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	8696	7.177997160497903	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	8641	7.132598144418398	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	7748	6.395483210618417	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	7341	6.059530491630072	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	6862	5.664146333410374	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	6517	5.3793706870934725	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	5183	4.278238188001453	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	3863	3.1886618020933075	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	3847	3.175454815597451	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	3475	2.868392379568792	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	3238	2.6727638920989203	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	3011	2.485389771188959	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2876	2.3739558226301716	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	2871	2.3698286393502164	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1842	1.5204543203354575	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1413	1.1663419949153102	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1406	1.160563938323373	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1233	1.0177633968369266	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1226	1.0119853402449897	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1186	0.9789678740053489	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1133	0.9352197312378248	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1016	0.8386436424868755	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	583	0.48122957044276427	No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	583	0.48122957044276427	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	514	0.4242744411793839	No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	444	0.36649387526001254	No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	382	0.3153168025885693	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	358	0.2955063228447849	No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	275	0.22699508039753027	No Hit
CCTACGGGAGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	270	0.22286789711757518	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	265	0.2187407138376201	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	263	0.21708984052563807	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	258	0.21296265724568297	No Hit
CCTACGGGGGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	236	0.19480305081388055	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	226	0.18654868425397036	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	215	0.17746888103806913	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	205	0.16921451447815894	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	195	0.16096014791824875	No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	188	0.1551820913263116	No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	186	0.15353121801432956	No Hit
CCTACGGGTGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	174	0.14362597814243733	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	140	0.11556113183874268	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGTGAAAGCCTGAT	136	0.11225938521477863	No Hit
CCTACGGGCGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	130	0.1073067652788325	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0
320-329	0.0	0.0	0.0	0.0	0.0
330-339	0.0	0.0022286789711757527	0.0	0.0	0.0
340-349	0.0	0.0024763099679730584	0.0	0.0	0.0
350-359	0.0	0.0024763099679730584	0.0	0.0	0.0
360-369	0.0	0.0024763099679730584	0.0	0.0	0.0
370-379	0.0	0.0024763099679730584	0.0	0.0	0.0
380-389	0.0	0.0024763099679730584	0.0	0.0	0.0
390-399	0.0	0.0024763099679730584	0.0	0.0	0.0
400-409	0.0	0.0024763099679730584	0.0	0.0	0.0
410-419	0.0	0.0024763099679730584	0.0	0.0	0.0
420-429	0.0	0.0024763099679730584	0.0	0.0	0.0
430-439	0.0	0.0024763099679730584	0.0	0.0	0.0
440-449	0.0	0.0024763099679730584	0.0	0.0	0.0
450-459	0.0	0.0024763099679730584	0.0	0.0	0.0
460-469	0.0	0.0024763099679730584	0.0	0.0	0.0
470-471	0.0	0.002476309967973058	0.0	0.0	0.0
>>END_MODULE
