FastQCFastQC Report
Tue 15 Oct 2024
ICMS1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameICMS1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188256
Sequences flagged as poor quality0
Sequence length321-491
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT122696.517189359170492No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT94715.0309153493115755No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT91084.838092809790924No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT89234.739822369539351No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT88284.689359170491246No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT80564.2792792792792795No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT78884.190039095699473No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT75654.018464218935917No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT65703.489928607853136No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT61483.2657657657657655No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT59213.145185279619242No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT59003.1340302566717657No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT56693.0113250042495325No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT51472.7340430052694202No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT51402.7303246642869285No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT50592.6872981472038076No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT50242.668706442291348No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT48602.5815910249872513No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT46022.4445436002039775No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT44692.373895121536631No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT42652.2655320414754376No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT41622.2108193098759137No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT32131.706718510963794No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT25371.3476330103688594No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT14040.7457929627740949No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10670.566781404045555No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9850.5232236953935068No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9300.49400815910249873No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9280.49294577596464384No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8680.4610742818289988No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8530.4531064082950876No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5260.27940676525582187No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT3230.171574876763556No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2930.15563912969573346No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2600.13810980792112867No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2550.13545385007649158No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2500.1327978922318545No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2500.1327978922318545No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2320.12323644399116096No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2310.12270525242223357No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2230.11845571987081421No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2050.10889427163012068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)