##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	ICMS1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	188256
Sequences flagged as poor quality	0
Sequence length	321-491
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.257760708822026	34.0	34.0	34.0	20.0	34.0
2	33.174273329933705	34.0	34.0	34.0	34.0	34.0
3	33.630837795342515	34.0	34.0	34.0	34.0	34.0
4	33.500669301376846	34.0	34.0	34.0	34.0	34.0
5	33.34111528981812	34.0	34.0	34.0	34.0	34.0
6	33.183245155532894	34.0	34.0	34.0	34.0	34.0
7	33.5756735509094	34.0	34.0	34.0	34.0	34.0
8	33.51452808941016	34.0	34.0	34.0	34.0	34.0
9	33.48626338602754	34.0	34.0	34.0	34.0	34.0
10-19	33.381814125446205	34.0	34.0	34.0	32.6	34.0
20-29	33.632279980452154	34.0	34.0	34.0	34.0	34.0
30-39	33.563951746557876	34.0	34.0	34.0	34.0	34.0
40-49	33.52782700152983	34.0	34.0	34.0	34.0	34.0
50-59	33.50502879058303	34.0	34.0	34.0	34.0	34.0
60-69	33.41464123321434	34.0	34.0	34.0	34.0	34.0
70-79	33.431303119156894	34.0	34.0	34.0	34.0	34.0
80-89	33.605346974332825	34.0	34.0	34.0	34.0	34.0
90-99	33.350994921808606	34.0	34.0	34.0	34.0	34.0
100-109	33.41566855770865	34.0	34.0	34.0	34.0	34.0
110-119	33.42347388662247	34.0	34.0	34.0	34.0	34.0
120-129	33.254946987081425	34.0	34.0	34.0	34.0	34.0
130-139	33.304669705082446	34.0	34.0	34.0	34.0	34.0
140-149	33.23929702107768	34.0	34.0	34.0	34.0	34.0
150-159	33.0659787735849	34.0	34.0	34.0	34.0	34.0
160-169	33.21170533741288	34.0	34.0	34.0	34.0	34.0
170-179	33.069282785143635	34.0	34.0	34.0	34.0	34.0
180-189	33.10842682304947	34.0	34.0	34.0	34.0	34.0
190-199	33.198568438721736	34.0	34.0	34.0	34.0	34.0
200-209	33.18237028301887	34.0	34.0	34.0	34.0	34.0
210-219	33.232386749957506	34.0	34.0	34.0	34.0	34.0
220-229	33.342482045724964	34.0	34.0	34.0	34.0	34.0
230-239	33.35387557368689	34.0	34.0	34.0	34.0	34.0
240-249	33.33430806986231	34.0	34.0	34.0	34.0	34.0
250-259	33.34758945266021	34.0	34.0	34.0	33.8	34.0
260-269	33.435972293047755	34.0	34.0	34.0	34.0	34.0
270-279	33.38163936342003	34.0	34.0	34.0	34.0	34.0
280-289	33.3387376763556	34.0	34.0	34.0	34.0	34.0
290-299	33.27878633350331	34.0	34.0	34.0	34.0	34.0
300-309	31.752516785653576	34.0	34.0	34.0	22.8	34.0
310-319	31.075909399966008	34.0	34.0	34.0	20.0	34.0
320-329	31.970271893901298	34.0	34.0	34.0	20.0	34.0
330-339	31.765871291599165	34.0	34.0	34.0	22.8	34.0
340-349	32.22196230662428	34.0	34.0	34.0	25.6	34.0
350-359	32.40269367202124	34.0	34.0	34.0	29.8	34.0
360-369	32.18182196824908	34.0	34.0	34.0	29.8	34.0
370-379	32.27019522430874	34.0	34.0	34.0	28.4	34.0
380-389	32.652686574212794	34.0	34.0	34.0	34.0	34.0
390-399	32.54862329272892	34.0	34.0	34.0	32.6	34.0
400-409	32.64099140976535	34.0	34.0	34.0	32.6	34.0
410-419	32.857953965614406	34.0	34.0	34.0	34.0	34.0
420-429	32.81787715434584	34.0	34.0	34.0	34.0	34.0
430-439	32.77193169813553	34.0	34.0	34.0	34.0	34.0
440-449	32.87384511731675	34.0	34.0	34.0	34.0	34.0
450-459	32.967671473065295	34.0	34.0	34.0	34.0	34.0
460-469	31.53789477688942	34.0	34.0	34.0	30.0	34.0
470-479	31.581111111111113	NaN	NaN	NaN	NaN	NaN
480-489	30.0	NaN	NaN	NaN	NaN	NaN
490-491	34.0	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.021164687445917707
1101	2	-0.003489706022129724
1101	3	0.0972865468209676
1101	4	0.11719307739659257
1101	5	-0.01173825449436805
1101	6	0.15480696972596775
1101	7	-0.021051697172033812
1101	8	-0.07574714240913494
1101	9	-0.08840476520728657
1101	10-19	-0.03077842237312467
1101	20-29	0.01817501809734523
1101	30-39	-0.0031028532114376617
1101	40-49	-8.814633898808211E-4
1101	50-59	-0.028038251763931044
1101	60-69	-0.05619229781780177
1101	70-79	-0.07167775524181508
1101	80-89	0.037324839769816265
1101	90-99	-0.11522111232141441
1101	100-109	0.06000287013299044
1101	110-119	0.024232496886504862
1101	120-129	-0.03364201774891029
1101	130-139	0.08188519978443765
1101	140-149	0.03129389824976414
1101	150-159	-0.08341103247641968
1101	160-169	-0.0732701832836895
1101	170-179	-0.33434388864593956
1101	180-189	-0.3176387383570898
1101	190-199	-0.1808530271568003
1101	200-209	-0.2512179539121746
1101	210-219	-0.2070663877491512
1101	220-229	-0.10297819344273051
1101	230-239	-0.19249126853325293
1101	240-249	-0.20570716317318727
1101	250-259	-0.09216673540283438
1101	260-269	-0.09356544991867821
1101	270-279	-0.15109098278414734
1101	280-289	-0.22103351194572696
1101	290-299	-0.30155811225884577
1101	300-309	-1.0495659117052014
1101	310-319	-1.107721428629393
1101	320-329	-0.9783827229548194
1101	330-339	-1.0474173788706196
1101	340-349	-0.7589646769605665
1101	350-359	-0.6466605050556637
1101	360-369	-0.4217450935849705
1101	370-379	-0.6459190476146546
1101	380-389	-0.6108459988710067
1101	390-399	-0.6128601547024317
1101	400-409	-0.5099342183763156
1101	410-419	-0.3961187724923292
1101	420-429	-0.4446269050928109
1101	430-439	-0.442733002015288
1101	440-449	-0.5341801070399583
1101	450-459	-0.45411519050660587
1101	460-469	-0.12632663168309222
1101	470-476	-3.2903225806451615
1102	1	-0.016367211056660835
1102	2	-0.2746630894300921
1102	3	0.06523824834192737
1102	4	0.05129625467469623
1102	5	0.10238214931258938
1102	6	0.10161069964395608
1102	7	-0.1364079171794188
1102	8	0.17400810178493487
1102	9	0.12877254499328217
1102	10-19	0.07752802794546199
1102	20-29	0.017901105588848054
1102	30-39	0.011495292529275503
1102	40-49	-0.004204257511460696
1102	50-59	-0.03498396679862026
1102	60-69	0.0783987861160611
1102	70-79	-0.04600086500476408
1102	80-89	-0.00791486042437839
1102	90-99	-0.0850827116066668
1102	100-109	-0.007213493999444154
1102	110-119	-0.018466290301660138
1102	120-129	0.035062140692353694
1102	130-139	0.1280258153385958
1102	140-149	-0.06859402231561518
1102	150-159	-0.15443114257640644
1102	160-169	-0.14260990434232212
1102	170-179	-0.3984661649016843
1102	180-189	-0.46837461073098297
1102	190-199	-0.24015455026014365
1102	200-209	-0.30144799762048535
1102	210-219	-0.2847048018385223
1102	220-229	-0.21608159006483874
1102	230-239	-0.1893059656512861
1102	240-249	-0.10886411183064126
1102	250-259	-0.16419315298031023
1102	260-269	-0.1301215351097511
1102	270-279	-0.2471778323088074
1102	280-289	-0.28450444351575044
1102	290-299	-0.39738308644579945
1102	300-309	-1.0514549450401347
1102	310-319	-1.3551773057111625
1102	320-329	-1.09395694949972
1102	330-339	-0.9879221910640084
1102	340-349	-0.7301982462224466
1102	350-359	-0.5647068989867954
1102	360-369	-0.7967446923459498
1102	370-379	-0.6101020447096843
1102	380-389	-0.2992328039918988
1102	390-399	-0.43848836127098423
1102	400-409	-0.4348808535858453
1102	410-419	-0.27801727506827945
1102	420-429	-0.33706194449447935
1102	430-439	-0.22660486383358602
1102	440-449	-0.2531250454221876
1102	450-459	-0.24354699525597567
1102	460-469	-0.1750563267126708
1102	470-476	-3.2903225806451615
1103	1	-0.5778290050765911
1103	2	-0.08031092996165512
1103	3	-0.24040959218963565
1103	4	0.11608030783084189
1103	5	-0.30459459487345697
1103	6	-0.23393747311352087
1103	7	-0.15135808329237221
1103	8	-0.0768224629991181
1103	9	-0.039001541385452754
1103	10-19	-0.17081084248643208
1103	20-29	-0.007846402716801038
1103	30-39	-0.06840503969663558
1103	40-49	-0.09357302827558556
1103	50-59	-0.07086436546973118
1103	60-69	0.006305762860243647
1103	70-79	-0.1484925593569315
1103	80-89	-0.04230024248418829
1103	90-99	-0.048537861108322033
1103	100-109	-0.010535753135656023
1103	110-119	-0.07544303448770506
1103	120-129	0.007985728732215591
1103	130-139	0.022876313677464566
1103	140-149	-0.24600266018936168
1103	150-159	-0.19745439839036294
1103	160-169	-0.21686239603667445
1103	170-179	-0.38451267652959586
1103	180-189	-0.3628928831562277
1103	190-199	-0.36955654361563006
1103	200-209	-0.3193881969560266
1103	210-219	-0.17772805765248023
1103	220-229	-0.213091556842258
1103	230-239	-0.18465480286058522
1103	240-249	-0.2263059722957621
1103	250-259	-0.1218343489936018
1103	260-269	-0.14208166800011668
1103	270-279	-0.11229411137857426
1103	280-289	-0.22669713454564544
1103	290-299	-0.20668541202720547
1103	300-309	-0.9571027855716956
1103	310-319	-0.8943799635184675
1103	320-329	-0.749604790031281
1103	330-339	-0.9349321578414127
1103	340-349	-0.6712281465546681
1103	350-359	-0.5696902876911061
1103	360-369	-0.6316284132761787
1103	370-379	-0.4865140048425687
1103	380-389	-0.3799637010018557
1103	390-399	-0.4310132782145004
1103	400-409	-0.43936590341972703
1103	410-419	-0.17668837141380322
1103	420-429	-0.3847363630991367
1103	430-439	-0.4365906486184201
1103	440-449	-0.2773707062867672
1103	450-459	-0.28236805668944953
1103	460-469	-1.5456870988649989
1103	470-476	-3.2903225806451615
1104	1	0.07274519640403554
1104	2	0.36811819081685115
1104	3	0.06405845395575938
1104	4	0.0682397762693796
1104	5	0.24032915012353584
1104	6	-0.010767985578532091
1104	7	0.13362896774484767
1104	8	-0.03819531464847614
1104	9	0.06954215809396658
1104	10-19	0.05199764366210502
1104	20-29	0.02725156468843437
1104	30-39	0.023906415557505056
1104	40-49	0.02172976817718819
1104	50-59	-0.06389913147280168
1104	60-69	-0.04564390622181236
1104	70-79	-0.09880621201081397
1104	80-89	0.041169033158695356
1104	90-99	-0.0858805769897728
1104	100-109	-0.016772967669886896
1104	110-119	-0.005227480036673171
1104	120-129	-0.06846494749138543
1104	130-139	-0.01832807065820674
1104	140-149	-0.2380425796180461
1104	150-159	-0.3061414520435335
1104	160-169	-0.36575297076134916
1104	170-179	-0.597665683566504
1104	180-189	-0.5009290879329598
1104	190-199	-0.3733525516727667
1104	200-209	-0.3997269627498383
1104	210-219	-0.3597943981971099
1104	220-229	-0.29663460233365413
1104	230-239	-0.20448842830856506
1104	240-249	-0.35273519771234874
1104	250-259	-0.1699422308572096
1104	260-269	-0.19731226417560066
1104	270-279	-0.25999939821772244
1104	280-289	-0.32234863741012276
1104	290-299	-0.37630217504810304
1104	300-309	-1.3650552798820001
1104	310-319	-1.6196851143281066
1104	320-329	-1.0219086590244046
1104	330-339	-1.1746718706098775
1104	340-349	-1.0346168508736113
1104	350-359	-0.7640003303311289
1104	360-369	-0.8470499739531832
1104	370-379	-0.6129447998304016
1104	380-389	-0.6299820126663889
1104	390-399	-0.6186293610878657
1104	400-409	-0.40335732309691963
1104	410-419	-0.5463184757902866
1104	420-429	-0.49795947765167625
1104	430-439	-0.3927219998342508
1104	440-449	-0.3122521942502061
1104	450-459	-0.34936176081313164
1104	460-469	0.04579930225028761
1104	470-476	-3.2903225806451615
1201	1	-0.2690527038584314
1201	2	-0.16175057558734807
1201	3	-0.2007971890888598
1201	4	-0.01176022373061869
1201	5	0.04060474067471631
1201	6	-6.761220614706076E-4
1201	7	0.0883927472724082
1201	8	0.056305997687481124
1201	9	-0.08581217261469476
1201	10-19	-0.057435427204040934
1201	20-29	-0.12840066628269398
1201	30-39	-0.06589673404879903
1201	40-49	-0.12311012683593248
1201	50-59	-0.11010910522608697
1201	60-69	-0.15477120280974077
1201	70-79	-0.1530279967877135
1201	80-89	-0.06602781723500328
1201	90-99	-0.20746587882704404
1201	100-109	-0.09061548735492408
1201	110-119	-0.1914164564146148
1201	120-129	-0.16561061678273603
1201	130-139	-0.11585956672120545
1201	140-149	-0.22096777646843435
1201	150-159	-0.23632649141360673
1201	160-169	-0.2846204248295905
1201	170-179	-0.47648720587621085
1201	180-189	-0.5524986417387296
1201	190-199	-0.41317669865438234
1201	200-209	-0.396043235715716
1201	210-219	-0.2851106711629825
1201	220-229	-0.23996531099506768
1201	230-239	-0.28602911072327686
1201	240-249	-0.3568834916756032
1201	250-259	-0.365364803035682
1201	260-269	-0.4255340489524997
1201	270-279	-0.4140322066166746
1201	280-289	-0.317945750271015
1201	290-299	-0.34856857924756923
1201	300-309	-1.4329765397245282
1201	310-319	-1.4643124109160333
1201	320-329	-1.3026186327776834
1201	330-339	-1.204984206567886
1201	340-349	-0.9642835175402737
1201	350-359	-0.9682168657641981
1201	360-369	-0.9245735960004389
1201	370-379	-0.8473489993054706
1201	380-389	-0.8054675769708481
1201	390-399	-0.7735348728988889
1201	400-409	-0.5774583729656939
1201	410-419	-0.6451767435068234
1201	420-429	-0.6398781128222879
1201	430-439	-0.6569704969811454
1201	440-449	-0.4967707341022276
1201	450-459	-0.32936859690288856
1201	460-469	-2.1120444755211984
1201	470-476	-3.2903225806451615
1202	1	-0.10077961148895298
1202	2	-0.08064615791666085
1202	3	0.018941766734435816
1202	4	-0.1326638381884564
1202	5	-0.25445550028914
1202	6	-0.042162065836571117
1202	7	-0.047992794896266844
1202	8	-0.144878741268343
1202	9	0.025272164734708724
1202	10-19	-0.09578482479439998
1202	20-29	-0.040061308852969546
1202	30-39	-0.0645714328439766
1202	40-49	-0.15774466498854878
1202	50-59	-0.09051472771605518
1202	60-69	-0.12135754819126987
1202	70-79	-0.11225892048248909
1202	80-89	-0.20113625096994525
1202	90-99	-0.20552411175876983
1202	100-109	-0.1530311500055319
1202	110-119	-0.11112729978811586
1202	120-129	-0.15377326738514796
1202	130-139	-0.16373908479349097
1202	140-149	-0.2633954873118114
1202	150-159	-0.25103733478710666
1202	160-169	-0.2793633967171374
1202	170-179	-0.49305748700071206
1202	180-189	-0.4817677180439546
1202	190-199	-0.4386305139154345
1202	200-209	-0.4737801835068822
1202	210-219	-0.3728837635324709
1202	220-229	-0.3291279615974858
1202	230-239	-0.28049497417708125
1202	240-249	-0.29280919448684983
1202	250-259	-0.29989693154973907
1202	260-269	-0.34386738228583624
1202	270-279	-0.34026313031145605
1202	280-289	-0.3805762723593702
1202	290-299	-0.41778946278170537
1202	300-309	-0.9838319614112763
1202	310-319	-1.311181888827683
1202	320-329	-0.9056929299664418
1202	330-339	-1.0090765760457963
1202	340-349	-0.7339843207531374
1202	350-359	-0.7553915645593818
1202	360-369	-0.89732058395224
1202	370-379	-0.7689975936829789
1202	380-389	-0.48196356090659265
1202	390-399	-0.6484806560314169
1202	400-409	-0.5958117866203025
1202	410-419	-0.4608996350730905
1202	420-429	-0.5681150606134437
1202	430-439	-0.5374812518608536
1202	440-449	-0.6179268508870877
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2404	1	0.24910968930396393
2404	2	-0.19115228498905878
2404	3	0.05621135791968612
2404	4	0.1485816566112561
2404	5	0.2783397834097556
2404	6	-0.0516590280443765
2404	7	0.0661277900074495
2404	8	-0.0726256262441396
2404	9	0.18965791285539524
2404	10-19	0.14323123946262228
2404	20-29	0.055355743973727556
2404	30-39	0.004146000993934251
2404	40-49	0.0733685056426907
2404	50-59	0.08477978366280325
2404	60-69	0.18093392539537945
2404	70-79	0.20538653312681276
2404	80-89	0.06742517421798055
2404	90-99	0.19516659980543238
2404	100-109	0.08936741862797248
2404	110-119	0.17803653503837324
2404	120-129	0.18082100714889293
2404	130-139	0.18218744182580338
2404	140-149	0.26848948848882515
2404	150-159	0.340156414569293
2404	160-169	0.308580429871256
2404	170-179	0.5198119394229366
2404	180-189	0.455672298432205
2404	190-199	0.4618745833968916
2404	200-209	0.4400294138569265
2404	210-219	0.3603038587515357
2404	220-229	0.3574107573809968
2404	230-239	0.32968883799468784
2404	240-249	0.34079599550388195
2404	250-259	0.353541179870291
2404	260-269	0.2938890279705717
2404	270-279	0.309647280562821
2404	280-289	0.36190467707941565
2404	290-299	0.37544424126524945
2404	300-309	1.307245682497701
2404	310-319	1.5304824608788437
2404	320-329	1.25053093987189
2404	330-339	1.279229468645795
2404	340-349	1.0284600722033161
2404	350-359	0.9911891171417864
2404	360-369	1.0108152928884593
2404	370-379	0.8982578383013617
2404	380-389	0.7362418247385492
2404	390-399	0.7497386313746119
2404	400-409	0.6874347894274777
2404	410-419	0.5249514616213844
2404	420-429	0.5500962461520729
2404	430-439	0.5471970814358968
2404	440-449	0.5860667661405472
2404	450-459	0.4404649849715767
2404	460-469	-0.6494183623211427
2404	470-476	-3.2903225806451615
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	9.0
18	50.0
19	80.0
20	176.0
21	329.0
22	366.0
23	458.0
24	610.0
25	816.0
26	1109.0
27	1460.0
28	1762.0
29	2472.0
30	3715.0
31	7236.0
32	25757.0
33	139471.0
34	2380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.07171124272927679	0.049401078324612886	0.09561499030570236	99.78327268864041
2	0.024435201563852902	0.10624000679936042	0.30544001954816125	99.56388477208863
3	0.00531191568927418	0.2480664626891042	99.66428692843787	0.0823346931837498
4	0.08870899201087881	99.70093914669387	0.029746727859935408	0.1806051334353221
5	0.17794917559068502	0.024966003739588644	0.14023457419683832	99.65685024647289
6	99.78964813870475	0.058431072582015985	0.02709077001529832	0.12483001869794323
7	99.89801121876593	0.05205677375488696	0.03612102668706442	0.013810980792112869
8	99.85976542580316	0.06002464728879823	0.04355770865204828	0.03665221825599184
9	30.421872344042157	21.057496175420702	25.94020907700153	22.58042240353561
10-19	47.62355515893252	17.803682219955803	14.375849906510286	20.196912714601396
20-29	37.48948240693524	27.53155277919429	29.942525072242056	5.036439741628421
30-39	28.41386197518273	32.2218680945096	10.075057368689444	29.28921256161822
40-49	29.535154258031614	33.26523457419684	12.495856705762366	24.70375446200918
50-59	32.5050994390617	25.621069182389938	14.083482066972634	27.790349311575728
60-69	35.886505609382965	10.141350076491586	21.303969063403024	32.66817525072242
70-79	40.07399498555159	37.025380333163355	8.655872853986061	14.244751827298996
80-89	37.00264533401326	3.0559982151963285	44.4943056263811	15.447050824409313
90-99	15.62675293217746	34.0647310045895	34.31486911439742	15.993646948835627
100-109	42.976638194798575	26.659495580486148	12.85472972972973	17.50913649498555
110-119	41.60876678565358	42.39891424443311	12.5226818799932	3.4696370899201088
120-129	24.267805541390448	35.12541432942376	29.431412544620088	11.175367584565697
130-139	18.86813700492946	17.978656722760498	33.27739886112527	29.87580741118477
140-149	22.45213963963964	25.713230919598846	26.510443226245112	25.324186214516402
150-159	28.229538500764917	42.08705167431582	3.8315910249872513	25.85181879993201
160-169	30.309100373958863	20.172159187489378	15.281265935747069	34.237474502804695
170-179	32.3265659527452	15.890489546149924	17.510942546319903	34.272001954784976
180-189	34.34668748937617	19.913681370049293	12.410016148223695	33.32961499235084
190-199	24.413670746217917	36.74007734149243	23.00760666326704	15.838645249022607
200-209	46.711698096770476	22.817400136092942	13.544169384187402	16.92673238294918
210-219	41.414722505524395	19.508169726330106	26.505874978752335	12.571232789393166
220-229	31.520479857980394	11.727825505416304	42.21269263028955	14.539002006313753
230-239	34.910812935577084	39.22228242393337	11.75680987591365	14.110094764575898
240-249	42.47227180010199	22.603529236783952	12.00423890872004	22.919960054394018
250-259	20.23514963246582	27.380844877097292	30.250628669897043	22.133376820539844
260-269	30.08722165561788	40.729166666666664	22.424570797212308	6.759040880503145
270-279	25.770599609043003	11.456102328743837	25.376721060683327	37.39657700152983
280-289	29.177662332143466	34.96515383307836	14.663915094339622	21.19326874043855
290-299	32.23514788373279	23.42544195138535	21.154385517593063	23.1850246472888
300-309	30.768846676865547	34.03206272310046	23.921946710861803	11.27714388917219
310-319	26.738430647628757	14.252666581676015	38.79886962434132	20.2100331463539
320-329	51.50986533174117	29.538520051504552	11.40584695668433	7.545767660069948
330-339	29.910068789673584	30.38830309951927	26.965658282648537	12.735969828158614
340-349	37.47575074421046	26.74679633599854	22.53087862517078	13.246574294620226
350-359	35.766590474106124	28.014178934412605	19.430005986684804	16.789224604796466
360-369	34.81453936971274	29.099934926094633	22.427495717074596	13.658029987118022
370-379	31.11694865733486	33.00709182182794	16.80363356264443	19.07232595819278
380-389	37.78162856371823	28.45173614726388	13.750741057901324	20.015894231116572
390-399	39.795260280813224	6.790303815893625	8.691557009971367	44.72287889332179
400-409	40.54346382485909	31.611797893081594	15.006560878042045	12.838177404017276
410-419	26.55511479515266	29.44293657990823	13.436533955108315	30.565414669830794
420-429	37.71636794051615	33.28679516725583	16.63091302994274	12.365923862285278
430-439	44.01389248751182	14.377011423314926	17.553683621368	24.055412467805258
440-449	23.439811495688215	45.578008248632415	20.26539010007232	10.716790155607047
450-459	16.715833304771248	33.22195449143416	16.674624196043766	33.38758800775082
460-469	32.813888200255946	16.84714805459333	33.516070459433564	16.822893285717157
470-479	26.153846153846157	26.153846153846157	30.76923076923077	16.923076923076923
480-489	21.428571428571427	14.285714285714285	32.142857142857146	32.142857142857146
490-491	0.0	0.0	50.0	50.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.2
46	0.8
47	3.0500000000000007
48	11.399999999999991
49	146.00000000000026
50	1820.6000000002769
51	8030.449999994888
52	2454.599999999959
53	1626.2500000002326
54	10706.400000003887
55	15590.050000021653
56	4049.849999998508
57	120.40000000000117
58	23.799999999999947
59	6.400000000000003
60	5.400000000000002
61	4.000000000000001
62	1.4
63	0.8
64	1.2
65	0.0
66	0.0
67	0.2
68	0.2
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.31191568927418E-4
2	0.001593574706782254
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0012217406085330614
210-219	0.0
220-229	1.593574706782254E-4
230-239	0.0
240-249	0.0
250-259	7.436681964983852E-4
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	1.0623978106105918E-4
350-359	0.0
360-369	0.0
370-379	0.0
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-489	0.0
490-491	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
320-324	1.0
325-329	0.0
330-334	0.0
335-339	0.0
340-344	3.0
345-349	0.0
350-354	1.0
355-359	0.0
360-364	5.0
365-369	1.0
370-374	0.0
375-379	0.0
380-384	0.0
385-389	6.0
390-394	0.0
395-399	2.0
400-404	0.0
405-409	0.0
410-414	2.0
415-419	0.0
420-424	2.0
425-429	6.0
430-434	8.0
435-439	52207.0
440-444	10478.0
445-449	35.0
450-454	46.0
455-459	9.0
460-464	6252.0
465-469	119182.0
470-474	5.0
475-479	2.0
480-484	0.0
485-489	2.0
490-492	1.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	3.5255184429712734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.361910501732716	1.916539180690124
2	12.068705740545427	0.8509688934217237
3	6.780171764351365	0.7171086180520142
4	4.50504746120235	0.6353051164371919
5	3.7818291396715384	0.6666454190039096
6	2.531264125357842	0.5354411014788374
7	2.018984480940184	0.49825769165391803
8	1.5971071267138766	0.45045045045045046
9	1.5217718848877504	0.48285313615502296
>10	9.733313243935513	6.29727604963454
>50	0.2862739189392798	0.644335373108958
>100	0.3314750640349555	2.187978072412035
>500	0.09040229019135151	2.7037650858405575
>1k	0.13560343528702729	16.243306986231516
>5k	0.24107277384360404	58.652579466258715
>10k+	0.015067048365225252	6.517189359170492
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	12269	6.517189359170492	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9471	5.0309153493115755	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9108	4.838092809790924	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8923	4.739822369539351	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8828	4.689359170491246	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8056	4.2792792792792795	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	7888	4.190039095699473	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	7565	4.018464218935917	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	6570	3.489928607853136	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	6148	3.2657657657657655	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	5921	3.145185279619242	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	5900	3.1340302566717657	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	5669	3.0113250042495325	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	5147	2.7340430052694202	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	5140	2.7303246642869285	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	5059	2.6872981472038076	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	5024	2.668706442291348	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4860	2.5815910249872513	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4602	2.4445436002039775	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4469	2.373895121536631	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4265	2.2655320414754376	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4162	2.2108193098759137	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3213	1.706718510963794	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	2537	1.3476330103688594	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1404	0.7457929627740949	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1067	0.566781404045555	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	985	0.5232236953935068	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	930	0.49400815910249873	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	928	0.49294577596464384	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	868	0.4610742818289988	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	853	0.4531064082950876	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	526	0.27940676525582187	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	323	0.171574876763556	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	293	0.15563912969573346	No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	260	0.13810980792112867	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	255	0.13545385007649158	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	250	0.1327978922318545	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	250	0.1327978922318545	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	232	0.12323644399116096	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	231	0.12270525242223357	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	223	0.11845571987081421	No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	205	0.10889427163012068	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0
320-329	0.0	0.0	0.0	0.0	0.0
330-339	0.0	5.311915689274178E-4	0.0	0.0	0.0
340-349	0.0	5.311915689274178E-4	0.0	0.0	0.0
350-359	0.0	5.311915689274178E-4	0.0	0.0	0.0
360-369	0.0	5.311915689274178E-4	0.0	0.0	0.0
370-379	0.0	5.311915689274178E-4	0.0	0.0	0.0
380-389	0.0	5.311915689274178E-4	0.0	0.0	0.0
390-399	0.0	5.311915689274178E-4	0.0	0.0	0.0
400-409	0.0	5.311915689274178E-4	0.0	0.0	0.0
410-419	0.0	5.311915689274178E-4	0.0	0.0	0.0
420-429	0.0	5.311915689274178E-4	0.0	0.0	0.0
430-439	0.0	5.311915689274178E-4	0.0	0.0	0.0
440-449	0.0	5.311915689274178E-4	0.0	0.0	0.0
450-459	0.0	5.311915689274178E-4	0.0	0.0	0.0
460-469	0.0	5.311915689274178E-4	0.0	0.0	0.0
470-479	0.0	5.311915689274178E-4	0.0	0.0	0.0
480	0.0	5.31191568927418E-4	0.0	0.0	0.0
>>END_MODULE
