##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	ICFS2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	167066
Sequences flagged as poor quality	0
Sequence length	316-478
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24523840877258	34.0	34.0	34.0	20.0	34.0
2	33.14725318137742	34.0	34.0	34.0	34.0	34.0
3	33.64137526486538	34.0	34.0	34.0	34.0	34.0
4	33.41315408281757	34.0	34.0	34.0	34.0	34.0
5	33.24774041396813	34.0	34.0	34.0	34.0	34.0
6	33.17840853315456	34.0	34.0	34.0	34.0	34.0
7	33.55411035159757	34.0	34.0	34.0	34.0	34.0
8	33.51534722804161	34.0	34.0	34.0	34.0	34.0
9	33.43333173715777	34.0	34.0	34.0	34.0	34.0
10-19	33.325193037482194	34.0	34.0	34.0	32.6	34.0
20-29	33.42065650700921	34.0	34.0	34.0	32.6	34.0
30-39	33.498091173548175	34.0	34.0	34.0	34.0	34.0
40-49	33.619170268037784	34.0	34.0	34.0	34.0	34.0
50-59	33.485351896855136	34.0	34.0	34.0	34.0	34.0
60-69	33.45995414985694	34.0	34.0	34.0	34.0	34.0
70-79	33.475329510492855	34.0	34.0	34.0	34.0	34.0
80-89	33.61423150132283	34.0	34.0	34.0	34.0	34.0
90-99	33.41328038020902	34.0	34.0	34.0	34.0	34.0
100-109	33.42398872301965	34.0	34.0	34.0	34.0	34.0
110-119	33.2914267415273	34.0	34.0	34.0	34.0	34.0
120-129	33.316292962062896	34.0	34.0	34.0	34.0	34.0
130-139	33.29970969556942	34.0	34.0	34.0	34.0	34.0
140-149	33.15087869464762	34.0	34.0	34.0	34.0	34.0
150-159	33.06700046688135	34.0	34.0	34.0	34.0	34.0
160-169	33.140853914022	34.0	34.0	34.0	34.0	34.0
170-179	33.05362790753354	34.0	34.0	34.0	34.0	34.0
180-189	33.1359720110615	34.0	34.0	34.0	34.0	34.0
190-199	33.21998192331175	34.0	34.0	34.0	34.0	34.0
200-209	33.22953204122922	34.0	34.0	34.0	34.0	34.0
210-219	33.24555505009995	34.0	34.0	34.0	34.0	34.0
220-229	33.35864628350471	34.0	34.0	34.0	34.0	34.0
230-239	33.37673075311554	34.0	34.0	34.0	34.0	34.0
240-249	33.40038368070104	34.0	34.0	34.0	34.0	34.0
250-259	33.380724384375036	34.0	34.0	34.0	34.0	34.0
260-269	33.37927166509044	34.0	34.0	34.0	34.0	34.0
270-279	33.33732716411478	34.0	34.0	34.0	34.0	34.0
280-289	33.30735397986425	34.0	34.0	34.0	34.0	34.0
290-299	33.331838315396304	34.0	34.0	34.0	34.0	34.0
300-309	31.90041121472951	34.0	34.0	34.0	22.8	34.0
310-319	31.382316343730896	34.0	34.0	34.0	20.0	34.0
320-329	31.886451170664213	34.0	34.0	34.0	20.0	34.0
330-339	32.09379010631166	34.0	34.0	34.0	21.4	34.0
340-349	32.11066947684551	34.0	34.0	34.0	22.8	34.0
350-359	32.455414703082056	34.0	34.0	34.0	34.0	34.0
360-369	32.34895402132773	34.0	34.0	34.0	29.8	34.0
370-379	32.13886494257346	34.0	34.0	34.0	24.2	34.0
380-389	32.52282654117805	34.0	34.0	34.0	34.0	34.0
390-399	32.52262165690537	34.0	34.0	34.0	32.6	34.0
400-409	32.62330064064439	34.0	34.0	34.0	34.0	34.0
410-419	32.80381943327706	34.0	34.0	34.0	34.0	34.0
420-429	32.78213856542026	34.0	34.0	34.0	34.0	34.0
430-439	32.71706578202293	34.0	34.0	34.0	34.0	34.0
440-449	32.574227020003306	34.0	34.0	34.0	31.2	34.0
450-459	32.87015433149567	34.0	34.0	34.0	34.0	34.0
460-469	32.31436233221964	34.0	34.0	34.0	28.0	34.0
470-478	34.0	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.09365761298194286
1101	2	0.26471093334335905
1101	3	0.18185791511773886
1101	4	0.053455831817252886
1101	5	-0.20899725892828513
1101	6	-0.19622726357081177
1101	7	-0.11519402102268828
1101	8	0.1422807051899042
1101	9	-0.018985830120627156
1101	10-19	0.016166558862011016
1101	20-29	-0.012905856821582518
1101	30-39	0.001380138042115675
1101	40-49	-0.0014945867600104634
1101	50-59	-0.0712820322605836
1101	60-69	0.006428958324704581
1101	70-79	-0.04016702040618014
1101	80-89	0.01289876843021176
1101	90-99	-0.056089943982854606
1101	100-109	-0.055121939569481526
1101	110-119	-0.16513490807177789
1101	120-129	-0.041272405254886735
1101	130-139	-0.08980860492670928
1101	140-149	-0.2467075063771702
1101	150-159	-0.34169558770246766
1101	160-169	-0.41608456122152404
1101	170-179	-0.34322068620494406
1101	180-189	-0.38451782934813394
1101	190-199	-0.30834837273174287
1101	200-209	-0.23519217139723025
1101	210-219	-0.30727813322948094
1101	220-229	-0.20717554825333906
1101	230-239	-0.2567010812292807
1101	240-249	-0.26556796203492183
1101	250-259	-0.1635244548911743
1101	260-269	-0.17987305479341842
1101	270-279	-0.20272250458408791
1101	280-289	-0.21152367815733442
1101	290-299	-0.2985666404186347
1101	300-309	-0.9666320596605615
1101	310-319	-1.1307861248648834
1101	320-329	-1.0255918901833603
1101	330-339	-0.7090267637408303
1101	340-349	-0.7210478152072675
1101	350-359	-0.5600362901107729
1101	360-369	-0.5363109066291045
1101	370-379	-0.6808120708492247
1101	380-389	-0.4101408970037568
1101	390-399	-0.356639497303874
1101	400-409	-0.4165035688174683
1101	410-419	-0.338207871977815
1101	420-429	-0.4152906326193673
1101	430-439	-0.44283139675577843
1101	440-449	-0.4209273004351175
1101	450-459	-0.41095023344846027
1101	460-468	-0.05241926243800776
1102	1	-0.3672777857909715
1102	2	-0.22555533362895375
1102	3	0.1860899729558625
1102	4	0.11124105488197245
1102	5	-0.21257687451636542
1102	6	-0.04359104420914406
1102	7	0.05715653443490254
1102	8	0.09736799138281071
1102	9	-0.0979313423759649
1102	10-19	-0.03855042227006322
1102	20-29	-0.05179670499651223
1102	30-39	-0.08078350152762681
1102	40-49	-0.04376050106083795
1102	50-59	-0.07940934666721233
1102	60-69	-0.08535203268217373
1102	70-79	-0.10489458168285637
1102	80-89	-0.02245654809244968
1102	90-99	-0.2058501273720239
1102	100-109	-0.14706339610273034
1102	110-119	-0.23511374778258443
1102	120-129	-0.20251028217253264
1102	130-139	-0.1545414562756875
1102	140-149	-0.5277143834711495
1102	150-159	-0.6340426164451927
1102	160-169	-0.47588882854650905
1102	170-179	-0.6226846255454461
1102	180-189	-0.5474555828307786
1102	190-199	-0.48965678394669254
1102	200-209	-0.45294389067072416
1102	210-219	-0.6748958373380987
1102	220-229	-0.5244158938713923
1102	230-239	-0.48639954710832
1102	240-249	-0.383887482401704
1102	250-259	-0.5204632663882549
1102	260-269	-0.5230118310233536
1102	270-279	-0.4561857385816097
1102	280-289	-0.5266356513542974
1102	290-299	-0.5759410012015778
1102	300-309	-1.3913860712987152
1102	310-319	-1.4166775020470403
1102	320-329	-1.2599209326802772
1102	330-339	-1.1472105055853028
1102	340-349	-0.9015115782120304
1102	350-359	-0.8133796158028836
1102	360-369	-0.7057818993993408
1102	370-379	-0.731503306962793
1102	380-389	-0.4731791618600454
1102	390-399	-0.3813423715764941
1102	400-409	-0.4714850535644217
1102	410-419	-0.24704934614462104
1102	420-429	-0.4474789591931554
1102	430-439	-0.3658149181309511
1102	440-449	-0.4250088378661303
1102	450-459	-0.22441002534619514
1102	460-468	-0.3433325118872368
1103	1	-0.2844448426701902
1103	2	-0.4024662274452382
1103	3	-0.2525066390810409
1103	4	-0.06849314150971253
1103	5	0.02774126348115402
1103	6	-0.19208987357149
1103	7	-0.022309103906920313
1103	8	-0.4896723611857041
1103	9	-0.045652042004114435
1103	10-19	-0.22711811267083704
1103	20-29	-0.007483249549601112
1103	30-39	-0.033909104061713435
1103	40-49	-0.008351466492605653
1103	50-59	-0.12226845698259581
1103	60-69	-0.01916555699011724
1103	70-79	-0.1184615834732412
1103	80-89	0.005744801583908554
1103	90-99	-0.11220393538744844
1103	100-109	-0.10596988281255193
1103	110-119	-0.1583633001858331
1103	120-129	-0.12739321840721374
1103	130-139	-0.15108256631561545
1103	140-149	-0.2441026220057836
1103	150-159	-0.530392292798318
1103	160-169	-0.5007008379116087
1103	170-179	-0.5215394664764474
1103	180-189	-0.3703422375615233
1103	190-199	-0.3795472948955947
1103	200-209	-0.3639843809613126
1103	210-219	-0.3731956582994016
1103	220-229	-0.17236314631595206
1103	230-239	-0.18180238418309358
1103	240-249	-0.30424624841189285
1103	250-259	-0.24100313692950692
1103	260-269	-0.2450094897480568
1103	270-279	-0.30379488332751947
1103	280-289	-0.37753773095766263
1103	290-299	-0.25531505436229196
1103	300-309	-0.6336557311251951
1103	310-319	-0.8160446691039205
1103	320-329	-0.7347060862403936
1103	330-339	-0.5435209311465243
1103	340-349	-0.5902830972865374
1103	350-359	-0.43662916820613873
1103	360-369	-0.6166192496265879
1103	370-379	-0.46097169974615326
1103	380-389	-0.5151286178578403
1103	390-399	-0.3156165762314913
1103	400-409	-0.3897070615523077
1103	410-419	-0.3199167197846222
1103	420-429	-0.37128422017649854
1103	430-439	-0.36005857754552295
1103	440-449	-0.4519886784880782
1103	450-459	-0.2978198345843879
1103	460-468	-0.1662959162864368
1104	1	-0.153316843386051
1104	2	-0.235365993510662
1104	3	-0.19388557474264445
1104	4	-0.10750460813888196
1104	5	0.08217579375842377
1104	6	-0.18442308333283108
1104	7	0.07764359784567176
1104	8	-0.029370930733300327
1104	9	-0.24760916678199862
1104	10-19	-0.026810674062687667
1104	20-29	-0.001404146426629893
1104	30-39	0.01927234224199026
1104	40-49	-0.07454760081962775
1104	50-59	-0.056862011637647925
1104	60-69	-0.09245674801112358
1104	70-79	-0.1646847545011525
1104	80-89	-0.09351824058823865
1104	90-99	-0.17702714220861537
1104	100-109	-0.15244488504963272
1104	110-119	-0.1784461999518996
1104	120-129	-0.11058284602939494
1104	130-139	-0.2585291640376326
1104	140-149	-0.427754366288454
1104	150-159	-0.5254499455269084
1104	160-169	-0.5149408062090046
1104	170-179	-0.8374511721535214
1104	180-189	-0.6506290950404079
1104	190-199	-0.5872399713372332
1104	200-209	-0.5946307690370389
1104	210-219	-0.42859309141073254
1104	220-229	-0.2796288601999066
1104	230-239	-0.42207875934768424
1104	240-249	-0.44275309387446526
1104	250-259	-0.43893863234669084
1104	260-269	-0.4332223190780766
1104	270-279	-0.50798397405066
1104	280-289	-0.5597420849530792
1104	290-299	-0.551770562051459
1104	300-309	-1.4680658452801438
1104	310-319	-1.5740409491606755
1104	320-329	-1.4140338758782427
1104	330-339	-1.2959459250744416
1104	340-349	-1.0133250137603866
1104	350-359	-0.9700220504590646
1104	360-369	-1.0168340092364367
1104	370-379	-0.8829526014808486
1104	380-389	-0.7366662583144645
1104	390-399	-0.6966637210791831
1104	400-409	-0.6092367635733495
1104	410-419	-0.5856350778962778
1104	420-429	-0.6187045481962343
1104	430-439	-0.4317625106678804
1104	440-449	-0.6900673487313185
1104	450-459	-0.8804937537268174
1104	460-468	-0.5761576644203714
1201	1	-0.39072574683783756
1201	2	0.17366103886039497
1201	3	-0.07198215120725138
1201	4	-0.18750361949417282
1201	5	-0.07229994207570911
1201	6	-0.012360064294355766
1201	7	-0.039998060817680425
1201	8	0.025205788096144488
1201	9	-0.06366475686165529
1201	10-19	-0.08149473146058028
1201	20-29	-0.09660498253758476
1201	30-39	-0.059475680468594305
1201	40-49	-0.08138831603344698
1201	50-59	-0.06801251160939614
1201	60-69	-0.01418115623664562
1201	70-79	-0.09739229927262727
1201	80-89	-0.10270380920017885
1201	90-99	-0.15880889182728453
1201	100-109	-0.1942469018848314
1201	110-119	-0.313385963242105
1201	120-129	-0.2110655164999855
1201	130-139	-0.40385216838766524
1201	140-149	-0.4561478892532591
1201	150-159	-0.4559750571025276
1201	160-169	-0.5202999667084143
1201	170-179	-0.515285051595356
1201	180-189	-0.6569062827699099
1201	190-199	-0.5542132355595868
1201	200-209	-0.5252437993743087
1201	210-219	-0.5889950461025677
1201	220-229	-0.5507876224167347
1201	230-239	-0.3974943432129905
1201	240-249	-0.5073278354144861
1201	250-259	-0.5924055974899076
1201	260-269	-0.5768805163550681
1201	270-279	-0.41993421697480215
1201	280-289	-0.6229609708917252
1201	290-299	-0.5840618167828211
1201	300-309	-1.507182632467309
1201	310-319	-1.618759820975825
1201	320-329	-1.551431127445948
1201	330-339	-1.4487275475816475
1201	340-349	-1.3707509438710517
1201	350-359	-1.1601892212917413
1201	360-369	-1.1903654709640499
1201	370-379	-1.041278416549222
1201	380-389	-1.01273128688387
1201	390-399	-1.0502560365278875
1201	400-409	-0.8700823472560657
1201	410-419	-0.6945469638309305
1201	420-429	-0.7318128642817854
1201	430-439	-0.6909159747161269
1201	440-449	-0.5733076814773632
1201	450-459	-0.53254725913785
1201	460-468	-2.4291978508877357
1202	1	0.10241647119842412
1202	2	0.21067245868615458
1202	3	-0.07367452229271976
1202	4	-0.021047886035255203
1202	5	-0.16933902848964522
1202	6	0.11265465197595148
1202	7	0.043384326750015134
1202	8	-0.16850447797402524
1202	9	0.11675671711997637
1202	10-19	-0.11543928055404962
1202	20-29	-0.05292120575397519
1202	30-39	-0.05361713325879691
1202	40-49	-0.0067091307261719635
1202	50-59	0.020979718199882313
1202	60-69	0.024265099944187796
1202	70-79	-0.0961988097506179
1202	80-89	-0.09881724221172306
1202	90-99	-0.09870028575240752
1202	100-109	-0.03918156164466069
1202	110-119	-0.001976438871963637
1202	120-129	-0.03031292643641592
1202	130-139	-0.12133804076817256
1202	140-149	-0.28370116215177177
1202	150-159	-0.2161587161559737
1202	160-169	-0.1760658014152554
1202	170-179	-0.3719044299800984
1202	180-189	-0.23694807697758336
1202	190-199	-0.3866640832167647
1202	200-209	-0.2461011692824897
1202	210-219	-0.2737431035548781
1202	220-229	-0.2210857740531793
1202	230-239	-0.1982257418473239
1202	240-249	-0.074319241112633
1202	250-259	-0.1935578814550638
1202	260-269	-0.14464452624440582
1202	270-279	-0.22113064975087582
1202	280-289	-0.2645815265780982
1202	290-299	-0.19235884998273178
1202	300-309	-0.7969768990084027
1202	310-319	-0.9408621873520921
1202	320-329	-0.908976159233088
1202	330-339	-0.6958931939202486
1202	340-349	-0.7096261629799727
1202	350-359	-0.5902788032426329
1202	360-369	-0.669173994152132
1202	370-379	-0.7480154953665874
1202	380-389	-0.5839242382958005
1202	390-399	-0.6148866492242036
1202	400-409	-0.5668968425742023
1202	410-419	-0.4394422672298788
1202	420-429	-0.5221966289356175
1202	430-439	-0.423119470938218
1202	440-449	-0.40380329900732903
1202	450-459	-0.5660599754999396
1202	460-468	-0.7081182664455987
1203	1	0.2910523646940959
1203	2	-0.029412945728871875
1203	3	-0.22139608616872408
1203	4	-0.3928304446215023
1203	5	-0.44413290537725914
1203	6	-0.4894316980934974
1203	7	0.17273212071555832
1203	8	-0.11842912113723969
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2404	320-329	1.3827564499557106
2404	330-339	1.1426911880437558
2404	340-349	1.1545827269171482
2404	350-359	0.9528205842592001
2404	360-369	0.9022761186062453
2404	370-379	1.0150914983216097
2404	380-389	0.8042734958613167
2404	390-399	0.7095068574946026
2404	400-409	0.7105831745063469
2404	410-419	0.6107982617336845
2404	420-429	0.6579882547697977
2404	430-439	0.5396561415673276
2404	440-449	0.7407338206282219
2404	450-459	0.5437080342680716
2404	460-468	0.4174899296120529
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	39.0
19	67.0
20	164.0
21	267.0
22	314.0
23	420.0
24	539.0
25	703.0
26	956.0
27	1251.0
28	1602.0
29	2098.0
30	3209.0
31	6427.0
32	23408.0
33	123390.0
34	2207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.10115762632731974	0.055666622771838675	0.12091029892377862	99.72226545197707
2	0.027535017359032684	0.11193583143780678	0.2777445229258949	99.58278462827727
3	0.004189960853794308	0.2519962170639149	99.65821890749763	0.08559491458465517
4	0.08559491458465517	99.70490704272564	0.027534028467791172	0.18196401422192426
5	0.16400703913423437	0.028731160140303833	0.20231525265463948	99.60494654807083
6	99.76775645553253	0.06045514946188931	0.03172398932158548	0.14006440568398118
7	99.88926532029258	0.06105371529814565	0.042498174374199414	0.007182790035075958
8	99.8395843558833	0.0622508469706583	0.04788526690050639	0.05027953024553171
9	30.25331306190368	22.521039589144408	25.352854560473105	21.872792788478804
10-19	47.87149988627249	16.65563310308501	14.94612907473693	20.52673793590557
20-29	38.95598146840171	27.711203955323043	30.307842409586634	3.024972166688614
30-39	39.20127374809955	28.458453545305446	10.362431613853209	21.97784109274179
40-49	24.85813989680725	35.616163671842266	12.07899871906911	27.446697712281377
50-59	32.65990686315588	26.424766260040943	7.973675074521447	32.94165180228173
60-69	40.16245076795997	12.780996731830536	15.540923946224847	31.515628553984655
70-79	38.66070894137646	39.71143141034082	13.05555888092131	8.572300767361401
80-89	32.912980498725055	6.219278608454144	47.14543952689356	13.722301365927239
90-99	10.457304298899835	33.382734967019026	36.137933511306905	20.022027222774234
100-109	36.6595836376043	29.779129206421413	16.16271413692792	17.398573019046363
110-119	34.03134090718638	45.85511115367579	12.076604455724086	8.03694348341374
120-129	29.03654842996181	26.514072282810385	23.029940263129543	21.41943902409826
130-139	25.152873714579865	35.35315384339123	14.058276369817916	25.435696072210984
140-149	21.49976655932386	28.404642476626	20.629212407072654	29.466378556977478
150-159	31.168699795290483	26.827361641506947	11.099625297786503	30.90431326541606
160-169	33.85027474171884	19.134174517855218	14.489064202171598	32.52648653825434
170-179	39.44297463277986	25.66075682664336	18.055439167754063	16.840829372822714
180-189	33.274753690158384	22.059784755725282	20.49657021775825	24.168891336358087
190-199	21.895658003423797	26.26345276716986	30.030107861563692	21.81078136784265
200-209	42.47944935709844	22.285558313665092	26.522465490542785	8.71252683869369
210-219	50.620174062945175	20.525181664731303	15.872529419510853	12.982114852812659
220-229	43.76972288597	14.829694825002035	23.32352476404365	18.077057524984316
230-239	35.469240825160895	35.12332880018388	18.608797981152314	10.798632393502912
240-249	29.211628937066784	32.474171884165536	19.288963643111106	19.025235535656567
250-259	26.675764894607205	24.10635349608387	20.320892105192275	28.89698950411665
260-269	27.200148444327393	27.243005758203342	18.554583218608215	27.002262578861046
270-279	18.065255647468664	29.11759424419092	30.494714663665857	22.32243544467456
280-289	25.714567895322805	22.257850190942502	31.5818299354746	20.445751978260088
290-299	41.52269163085248	23.904744232818167	22.02243424754289	12.550129888786469
300-309	45.498904624519646	30.96315228712006	13.445644236409562	10.092298851950726
310-319	22.19522020378809	21.69601539992949	33.28654535311557	22.82221904316685
320-329	49.86564441893081	21.848091097214297	19.149725811655564	9.13653867219932
330-339	29.788274590556462	34.72913274590557	24.05097691791974	11.431615745618236
340-349	35.993387832030336	32.68053840912819	24.636971382795668	6.6891023760458
350-359	26.21274750911533	37.329347678337996	10.790969281833593	25.666935530713076
360-369	49.69469529710396	23.820713054298547	10.181973219894237	16.30261842870325
370-379	31.205111038986704	18.348229951244164	23.282018546618648	27.16464046315048
380-389	24.584622312605468	33.32674621510175	19.66063166870867	22.42799980358411
390-399	42.05256728108943	13.277759812199823	16.37634292695196	28.29332997975878
400-409	40.79121197639503	30.38396998954377	12.752048426834541	16.072769607226654
410-419	34.81929753004122	37.95653270229267	7.593630418363906	19.630539349302207
420-429	30.674128565002196	38.2440059923604	22.976065899192495	8.105799543444911
430-439	28.289162867550953	18.405006918747286	23.179362228196354	30.126467985505407
440-449	22.597891210223782	30.33546779063406	23.283653164197858	23.782987834944308
450-459	17.41891779962846	32.203625477737155	16.765468034503566	33.61198868813082
460-469	31.84368545181833	17.201569609769155	33.90417704066946	17.050567897743054
470-478	18.181818181818183	18.181818181818183	27.27272727272727	36.36363636363637
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.2
41	0.0
42	0.0
43	0.2
44	0.0
45	0.2
46	1.05
47	51.100000000000186
48	239.09999999999496
49	993.6000000000889
50	2206.0000000001846
51	3700.8499999988253
52	2772.0499999996705
53	6183.399999996568
54	10344.25000000257
55	7494.699999995379
56	4349.699999998236
57	1683.5000000002456
58	1699.7000000002492
59	31.19999999999992
60	3.2000000000000006
61	1.65
62	0.8
63	1.5999999999999999
64	6.200000000000003
65	0.4
66	0.4
67	0.25
68	0.2
69	0.2
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003591395017537979
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0018555540923946225
210-219	0.0
220-229	7.182790035075959E-4
230-239	2.3942633450253195E-4
240-249	0.0
250-259	2.992829181281649E-4
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	5.988059808741369E-5
360-369	0.0
370-379	5.988185310382615E-5
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-478	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
315-319	8.0
320-324	2.0
325-329	0.0
330-334	0.0
335-339	0.0
340-344	56.0
345-349	1.0
350-354	0.0
355-359	0.0
360-364	3.0
365-369	0.0
370-374	1.0
375-379	0.0
380-384	1.0
385-389	8.0
390-394	0.0
395-399	2.0
400-404	2.0
405-409	3.0
410-414	20.0
415-419	4.0
420-424	10.0
425-429	2.0
430-434	9.0
435-439	24179.0
440-444	107979.0
445-449	119.0
450-454	950.0
455-459	6.0
460-464	4659.0
465-469	29040.0
470-474	1.0
475-479	1.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.650066440807824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.7992799279928	4.176193839560413
2	14.518451845184519	1.93097338776292
3	6.831683168316832	1.362934409155663
4	3.7353735373537353	0.9936192881855075
5	2.286228622862286	0.7601786120455389
6	1.6831683168316833	0.6715908682796021
7	1.2421242124212422	0.5782145978236146
8	0.729072907290729	0.38787066189410174
9	0.567056705670567	0.339386829157339
>10	3.4563456345634562	4.470089665162271
>50	0.6750675067506751	3.200531526462596
>100	0.9000900090009001	10.482084924520848
>500	0.18901890189018902	10.298325212790155
>1k	0.36003600360036003	50.09098200711096
>5k	0.027002700270027002	10.257024170088467
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	6254	3.7434307399470867	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	5667	3.3920725940646212	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	5215	3.12152083607676	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	4943	2.9587109286150386	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	4833	2.892868686626842	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	4633	2.7731555193755764	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	4024	2.408628925095471	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	3876	2.3200411813295343	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	3035	1.8166473130379608	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	2747	1.644260352196138	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2630	1.5742281493541475	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	2418	1.4473321920678055	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	2351	1.4072282810386314	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	2339	1.4000454910035554	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2268	1.357547316629356	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	2194	1.3132534447463877	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	2174	1.3012821280212612	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2118	1.2677624411909065	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2019	1.20850442340153	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1981	1.1857589216237894	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1895	1.1342822597057451	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	1887	1.1294937330156942	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	1845	1.1043539678929286	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	1810	1.083404163623957	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1675	1.0025977757293525	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	1649	0.9870350639866878	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	1639	0.9810494056241246	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	1635	0.9786551422790992	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1604	0.960099601355153	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1563	0.9355584020686435	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	1518	0.9086229394371087	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	1369	0.8194366298349156	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1367	0.8182394981624029	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	1297	0.7763398896244598	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	1282	0.7673614020806148	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1227	0.7344402810865167	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1215	0.7272574910514408	No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1184	0.7087019501274945	No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1160	0.6943363700573426	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	1152	0.6895478433672919	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1093	0.6542324590281685	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1022	0.6117342846539692	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	1014	0.6069457579639184	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	993	0.5943758754025356	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	982	0.5877916512037159	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	956	0.5722289394610512	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	951	0.5692361102797696	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	945	0.5656447152622317	No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	941	0.5632504519172064	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	912	0.5458920426657728	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	890	0.5327235942681335	No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	889	0.5321250284318773	No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	843	0.5045909999640861	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	825	0.4938168149114721	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	790	0.4728670106425006	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	776	0.4644870889349119	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	756	0.4525157722097854	No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	721	0.43156596794081376	No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	717	0.42917170459578846	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	711	0.4255803095782505	No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	699	0.41839751954317456	No Hit
CCTACGGGCGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	694	0.4154046903618929	No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	620	0.3711108184789245	No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	594	0.3555481067362599	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	332	0.19872385763710151	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGGGCAACCCTGAC	309	0.18495684340320592	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	305	0.1825625800581806	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC	289	0.17298552667807932	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	288	0.17238696084182298	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC	271	0.16221134162546538	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	266	0.15921851244418372	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGGGCAACCCTGAC	266	0.15921851244418372	No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA	262	0.15682424909915843	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	257	0.15383141991787677	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGGGCAACCCTGAC	254	0.15203572240910776	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGCAACCCTGAC	253	0.15143715657285145	No Hit
CCTACGGGCGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA	253	0.15143715657285145	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC	252	0.1508385907365951	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC	249	0.14904289322782613	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGGGCAACCCTGAC	248	0.14844432739156982	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC	247	0.14784576155531345	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	243	0.14545149821028816	No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA	240	0.14365580070151918	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	239	0.1430572348652628	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGGGCAACCCTGAC	227	0.13587444483018687	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	225	0.13467731315767423	No Hit
CCTACGGGTGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA	223	0.13348018148516155	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGGGCAACCCTGAC	221	0.1322830498126489	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	216	0.12929022063136725	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	215	0.1286916547951109	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGCAACCCTGAC	212	0.12689595728634193	No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA	212	0.12689595728634193	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC	211	0.12629739145008562	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	210	0.12569882561382925	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC	208	0.1245016939413166	No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC	206	0.12330456226880394	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	200	0.11971316725126596	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	200	0.11971316725126596	No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA	199	0.11911460141500964	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC	193	0.11552320639747166	No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC	188	0.11253037721619	No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC	185	0.11073467970742101	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	183	0.10953754803490837	No Hit
CCTACGGGCGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC	183	0.10953754803490837	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	179	0.10714328468988303	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT	176	0.10534758718111406	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC	176	0.10534758718111406	No Hit
CCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA	174	0.1041504555086014	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC	173	0.10355188967234505	No Hit
CCTACGGGGGGCAGCAGTTAGGAATATTCGTCAATGGGGGAAACCCTGAA	173	0.10355188967234505	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC	171	0.1023547579998324	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC	170	0.10175619216357606	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0
310-319	0.0	5.985658362563297E-4	0.0	0.0	0.0
320-329	0.0	6.584224198819628E-4	0.0	0.0	0.0
330-339	0.0	0.0017956975087689898	0.0	0.0	0.0
340-349	0.0	0.0017956975087689898	0.0	0.0	0.0
350-359	0.0	0.0017956975087689898	0.0	0.0	0.0
360-369	0.0	0.0017956975087689898	0.0	0.0	0.0
370-379	0.0	0.0017956975087689898	0.0	0.0	0.0
380-389	0.0	0.0017956975087689898	0.0	0.0	0.0
390-399	0.0	0.0017956975087689898	0.0	0.0	0.0
400-409	0.0	0.0017956975087689898	0.0	0.0	0.0
410-419	0.0	0.0017956975087689898	0.0	0.0	0.0
420-429	0.0	0.0017956975087689898	0.0	0.0	0.0
430-439	0.0	0.0017956975087689898	0.0	0.0	0.0
440-449	0.0	0.0017956975087689898	0.0	0.0	0.0
450-459	0.0	0.0017956975087689898	0.0	0.0	0.0
460-467	0.0	0.0017956975087689896	0.0	0.0	0.0
>>END_MODULE
