##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	ICFS1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	134460
Sequences flagged as poor quality	0
Sequence length	319-484
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24209430313848	34.0	34.0	34.0	20.0	34.0
2	33.13456790123457	34.0	34.0	34.0	34.0	34.0
3	33.62008775844117	34.0	34.0	34.0	34.0	34.0
4	33.440807675145024	34.0	34.0	34.0	34.0	34.0
5	33.291960434329916	34.0	34.0	34.0	34.0	34.0
6	33.14556745500521	34.0	34.0	34.0	34.0	34.0
7	33.54294957608211	34.0	34.0	34.0	34.0	34.0
8	33.50212702662502	34.0	34.0	34.0	34.0	34.0
9	33.444117209579055	34.0	34.0	34.0	34.0	34.0
10-19	33.41085081065	34.0	34.0	34.0	34.0	34.0
20-29	33.63785884277852	34.0	34.0	34.0	34.0	34.0
30-39	33.57913357132232	34.0	34.0	34.0	34.0	34.0
40-49	33.54185333928306	34.0	34.0	34.0	34.0	34.0
50-59	33.50334077048936	34.0	34.0	34.0	34.0	34.0
60-69	33.445879071842924	34.0	34.0	34.0	34.0	34.0
70-79	33.509756804997764	34.0	34.0	34.0	34.0	34.0
80-89	33.64049010858248	34.0	34.0	34.0	34.0	34.0
90-99	33.342780752640195	34.0	34.0	34.0	34.0	34.0
100-109	33.42581659973227	34.0	34.0	34.0	34.0	34.0
110-119	33.336636174326934	34.0	34.0	34.0	34.0	34.0
120-129	33.37505949724825	34.0	34.0	34.0	34.0	34.0
130-139	33.335831474044326	34.0	34.0	34.0	34.0	34.0
140-149	33.29340770489365	34.0	34.0	34.0	34.0	34.0
150-159	33.13244013089394	34.0	34.0	34.0	34.0	34.0
160-169	33.21854231741782	34.0	34.0	34.0	34.0	34.0
170-179	33.05395284843076	34.0	34.0	34.0	33.1	34.0
180-189	33.11778075264019	34.0	34.0	34.0	34.0	34.0
190-199	33.170060984679466	34.0	34.0	34.0	34.0	34.0
200-209	33.218091625762305	34.0	34.0	34.0	34.0	34.0
210-219	33.24760300461104	34.0	34.0	34.0	34.0	34.0
220-229	33.316214487579956	34.0	34.0	34.0	34.0	34.0
230-239	33.3633006098468	34.0	34.0	34.0	34.0	34.0
240-249	33.3585393425554	34.0	34.0	34.0	34.0	34.0
250-259	33.313836085081064	34.0	34.0	34.0	33.8	34.0
260-269	33.408420348058904	34.0	34.0	34.0	34.0	34.0
270-279	33.36484976944816	34.0	34.0	34.0	34.0	34.0
280-289	33.325293023947644	34.0	34.0	34.0	34.0	34.0
290-299	33.27002751747732	34.0	34.0	34.0	34.0	34.0
300-309	31.81357057861074	34.0	34.0	34.0	22.8	34.0
310-319	31.27829689126878	34.0	34.0	34.0	20.0	34.0
320-329	31.824969879010116	34.0	34.0	34.0	20.0	34.0
330-339	31.957756440768726	34.0	34.0	34.0	21.4	34.0
340-349	32.031072681520286	34.0	34.0	34.0	20.0	34.0
350-359	32.29871558995558	34.0	34.0	34.0	27.0	34.0
360-369	32.0997630510842	34.0	34.0	34.0	25.6	34.0
370-379	32.120509959685215	34.0	34.0	34.0	27.0	34.0
380-389	32.50565930233236	34.0	34.0	34.0	32.6	34.0
390-399	32.39885448202504	34.0	34.0	34.0	31.2	34.0
400-409	32.65245472324396	34.0	34.0	34.0	32.6	34.0
410-419	32.91361615089468	34.0	34.0	34.0	34.0	34.0
420-429	32.85345368147198	34.0	34.0	34.0	34.0	34.0
430-439	32.80769535919645	34.0	34.0	34.0	34.0	34.0
440-449	32.93766985006458	34.0	34.0	34.0	34.0	34.0
450-459	32.99077003882982	34.0	34.0	34.0	34.0	34.0
460-469	32.0832684196247	34.0	34.0	34.0	30.0	34.0
470-479	33.25	NaN	NaN	NaN	NaN	NaN
480-484	34.0	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.04307736478369151
1101	2	0.00552678551508734
1101	3	-0.11714016194259358
1101	4	-0.14746222043854829
1101	5	-0.15877767625644879
1101	6	-0.21279496133432474
1101	7	-0.18366997578936406
1101	8	-0.3139165632690535
1101	9	-0.17235210518633437
1101	10-19	-0.048113551458847326
1101	20-29	-0.09339632532116582
1101	30-39	-0.07498853085666468
1101	40-49	-0.14080133081416335
1101	50-59	-0.12111532622051158
1101	60-69	-0.19142930002754355
1101	70-79	-0.10155090612111195
1101	80-89	-0.054103642957528564
1101	90-99	-0.14906861401545513
1101	100-109	-0.1341870519717645
1101	110-119	-0.17209420846950252
1101	120-129	-0.12545149810849665
1101	130-139	-0.1054967611069344
1101	140-149	-0.1509074874319225
1101	150-159	-0.30388068430401205
1101	160-169	-0.44187970633416285
1101	170-179	-0.514515689908734
1101	180-189	-0.5464606957925469
1101	190-199	-0.5120723479370781
1101	200-209	-0.3814896864488304
1101	210-219	-0.44712327634483273
1101	220-229	-0.3594943648931803
1101	230-239	-0.4578211378156922
1101	240-249	-0.332931156254169
1101	250-259	-0.3581843902164721
1101	260-269	-0.3281022863313581
1101	270-279	-0.28518815240729367
1101	280-289	-0.48485159420685875
1101	290-299	-0.5572622180542695
1101	300-309	-1.1230438713560211
1101	310-319	-1.284326030637395
1101	320-329	-1.134414021717479
1101	330-339	-1.0609723141204057
1101	340-349	-0.9587600573760575
1101	350-359	-1.0884194704980494
1101	360-369	-0.7510672692076206
1101	370-379	-0.8818195396287152
1101	380-389	-0.5394919575606671
1101	390-399	-0.6096085690282464
1101	400-409	-0.6502651410989628
1101	410-419	-0.47932453140623466
1101	420-429	-0.5925343952341464
1101	430-439	-0.3607793258464085
1101	440-449	-0.556854940409977
1101	450-459	-0.4069336382681712
1101	460-469	-0.003953531714337544
1101	470-475	-4.387096774193548
1102	1	0.35645602965273326
1102	2	0.19319348149093685
1102	3	0.074278109401817
1102	4	0.06309494712204611
1102	5	-0.12165271167170744
1102	6	-0.12012909578531605
1102	7	-0.10810601826813837
1102	8	-0.2253686378706945
1102	9	0.09472293609076843
1102	10-19	-0.12303368393636305
1102	20-29	0.01625421152535722
1102	30-39	-0.02184316708085987
1102	40-49	-0.13246921869676243
1102	50-59	-0.15875662090480347
1102	60-69	-0.04588283031857543
1102	70-79	-0.09947581599538324
1102	80-89	-0.11556841820493702
1102	90-99	-0.2303214838729133
1102	100-109	-0.1577086479544434
1102	110-119	-0.15641672671446116
1102	120-129	-0.12517354936048974
1102	130-139	-0.18204648413515656
1102	140-149	-0.21653172417195776
1102	150-159	-0.37183646651724445
1102	160-169	-0.5103967593935437
1102	170-179	-0.6389204692594319
1102	180-189	-0.38730597261778144
1102	190-199	-0.44169025389368244
1102	200-209	-0.2966337410811519
1102	210-219	-0.34052138297164447
1102	220-229	-0.350977116439239
1102	230-239	-0.3206370663794331
1102	240-249	-0.37979360826037833
1102	250-259	-0.3792616003730913
1102	260-269	-0.33927348126673706
1102	270-279	-0.4434445491363874
1102	280-289	-0.6472801530743553
1102	290-299	-0.7169518338108887
1102	300-309	-1.4078333825765519
1102	310-319	-1.623483098546771
1102	320-329	-1.503273503726522
1102	330-339	-1.2010814419768359
1102	340-349	-1.3579648015557417
1102	350-359	-1.1417822107112237
1102	360-369	-1.008655416574193
1102	370-379	-0.9550626983770272
1102	380-389	-0.6226254999661727
1102	390-399	-0.7093672567584441
1102	400-409	-0.7055193005604536
1102	410-419	-0.5806859429364621
1102	420-429	-0.47540366651016797
1102	430-439	-0.4906694509119518
1102	440-449	-0.3805740378346485
1102	450-459	-0.3586142180545977
1102	460-469	0.3804296576158066
1102	470-475	-4.387096774193548
1103	1	-0.24668298380018427
1103	2	0.14191599903713836
1103	3	-0.2364753132216535
1103	4	0.16998573004407547
1103	5	-0.11083296489140082
1103	6	-0.05555708457950459
1103	7	-0.07512707371315486
1103	8	-0.028816504113677865
1103	9	-0.12061279283151549
1103	10-19	-0.08794820496705569
1103	20-29	0.017271841872116056
1103	30-39	0.009782566691995953
1103	40-49	-0.0075704372794049846
1103	50-59	-0.013342886854779579
1103	60-69	0.05455174139564889
1103	70-79	0.020770870271562103
1103	80-89	0.06366172661929426
1103	90-99	0.059620012281271784
1103	100-109	0.1560075836455468
1103	110-119	0.053980327305076514
1103	120-129	0.139983005347041
1103	130-139	0.1426528298552796
1103	140-149	0.026557199693847622
1103	150-159	0.023348102722202668
1103	160-169	-0.10142319996413818
1103	170-179	-0.25195960051196664
1103	180-189	-0.16656630978208398
1103	190-199	-0.12398566366808694
1103	200-209	-0.06780748067469489
1103	210-219	-0.2125390232175306
1103	220-229	-0.03219055153560646
1103	230-239	-0.027390746380234532
1103	240-249	-0.07888189858200434
1103	250-259	0.04628111931331347
1103	260-269	-0.03665961654821359
1103	270-279	0.03362758580852443
1103	280-289	-0.06064694545993632
1103	290-299	-0.008736052621216572
1103	300-309	-0.6039568840960747
1103	310-319	-0.7391544079044188
1103	320-329	-0.4781341735015161
1103	330-339	-0.5468327362877972
1103	340-349	-0.4786737770949898
1103	350-359	-0.2903210994351326
1103	360-369	-0.4442759434057777
1103	370-379	-0.42235251514416916
1103	380-389	-0.22388070938222882
1103	390-399	-0.2713090696847189
1103	400-409	-0.2365208943200443
1103	410-419	-0.07506967272986032
1103	420-429	-0.16000903777697317
1103	430-439	-0.1606746051233756
1103	440-449	-0.22454389625121962
1103	450-459	-0.12524176405291598
1103	460-469	-0.6608800553902157
1103	470-475	-4.387096774193548
1104	1	0.013498011674926147
1104	2	-0.01659488366540529
1104	3	0.0329352528387048
1104	4	-0.12458640344508609
1104	5	0.26664548446083103
1104	6	0.046967063133585896
1104	7	0.03838465802997604
1104	8	-0.11649742467344737
1104	9	0.07668426149709262
1104	10-19	-0.03207534130299905
1104	20-29	0.034113311011992664
1104	30-39	-0.03855481207000366
1104	40-49	0.03352412586423981
1104	50-59	0.03339280181702975
1104	60-69	7.275627175644672E-4
1104	70-79	-0.06233521984660939
1104	80-89	-0.07004967949178109
1104	90-99	-0.13296336849845147
1104	100-109	-0.09825609839374749
1104	110-119	-0.12918319791143063
1104	120-129	-0.006480490902191605
1104	130-139	-0.09984065250964846
1104	140-149	-0.31713842425109107
1104	150-159	-0.36794973072599646
1104	160-169	-0.30795981359074176
1104	170-179	-0.6523571512796025
1104	180-189	-0.42139785014147435
1104	190-199	-0.6221946874711932
1104	200-209	-0.4150995406469207
1104	210-219	-0.37769140103060295
1104	220-229	-0.2580363457880992
1104	230-239	-0.2649687831986256
1104	240-249	-0.2835596127649822
1104	250-259	-0.3305490625811487
1104	260-269	-0.2907719695892865
1104	270-279	-0.288808061909549
1104	280-289	-0.38424827595648026
1104	290-299	-0.4363723236349628
1104	300-309	-1.6195412737357806
1104	310-319	-1.5513446329501157
1104	320-329	-1.4751715212985026
1104	330-339	-1.1749659457610981
1104	340-349	-1.1876271597725676
1104	350-359	-1.0866262745872035
1104	360-369	-1.1219571636269308
1104	370-379	-0.9384727992231561
1104	380-389	-0.8335258104180561
1104	390-399	-0.7656249926917305
1104	400-409	-0.5894640685331822
1104	410-419	-0.4059208109436838
1104	420-429	-0.5329650974295532
1104	430-439	-0.4362369487738178
1104	440-449	-0.4569998128073536
1104	450-459	-0.26213815422480735
1104	460-469	-0.23946046103874608
1104	470-475	-4.387096774193548
1201	1	-0.8898547899675862
1201	2	-0.4445754286451731
1201	3	-0.2835476941184609
1201	4	-0.11771933943886381
1201	5	-0.18829501427339324
1201	6	-0.1393207356185826
1201	7	-0.14224204876931879
1201	8	-0.21709144066004882
1201	9	-0.015527750383228067
1201	10-19	-0.04683803390968677
1201	20-29	-0.016978043862721393
1201	30-39	-0.03710066890816677
1201	40-49	-0.12616551074947324
1201	50-59	-0.07849250127066654
1201	60-69	-0.203379718961628
1201	70-79	-0.1693707212718465
1201	80-89	-0.017856006360041476
1201	90-99	-0.15953860720117063
1201	100-109	-0.12721537441184694
1201	110-119	-0.2545983114322752
1201	120-129	-0.32126311693124876
1201	130-139	-0.18550453514166065
1201	140-149	-0.2519142729165509
1201	150-159	-0.32896394325615574
1201	160-169	-0.28386429263074575
1201	170-179	-0.5259732734316174
1201	180-189	-0.5744486604350882
1201	190-199	-0.41180937441678367
1201	200-209	-0.39217610492224253
1201	210-219	-0.5492035504515442
1201	220-229	-0.35132874916084234
1201	230-239	-0.35656385565661
1201	240-249	-0.4625487889166209
1201	250-259	-0.5005432818886675
1201	260-269	-0.42225880356381396
1201	270-279	-0.43947682823004186
1201	280-289	-0.42199420455496295
1201	290-299	-0.44411546155431836
1201	300-309	-1.4191774887775352
1201	310-319	-1.8626249566688955
1201	320-329	-1.7101945444013467
1201	330-339	-1.4946631570753048
1201	340-349	-1.3369922802701026
1201	350-359	-1.0958743858954314
1201	360-369	-1.2508167725331774
1201	370-379	-1.160493250914513
1201	380-389	-1.0304759967955555
1201	390-399	-0.9777993304037658
1201	400-409	-0.859027725917521
1201	410-419	-0.6315502087549945
1201	420-429	-0.5697113648962144
1201	430-439	-0.5651861978955353
1201	440-449	-0.4147398173330714
1201	450-459	-0.5650250589867127
1201	460-469	-0.09894414069263746
1201	470-475	-4.387096774193548
1202	1	0.6339140311251867
1202	2	0.153414796440849
1202	3	0.12898216758622993
1202	4	-0.0874192977968562
1202	5	0.39552596891638103
1202	6	0.29136723422383426
1202	7	0.21835584282995768
1202	8	0.071476731694041
1202	9	-0.1881229242934097
1202	10-19	0.07980079836067233
1202	20-29	0.10264958889857212
1202	30-39	0.09867420123451609
1202	40-49	0.13857466502364701
1202	50-59	0.09405555141743349
1202	60-69	0.09570854018317476
1202	70-79	0.07385149364476007
1202	80-89	0.08346667538540231
1202	90-99	0.03946746376556831
1202	100-109	0.12328213802597077
1202	110-119	0.03713903706751864
1202	120-129	0.01658179945597027
1202	130-139	0.03483101179327264
1202	140-149	-0.02436698731811049
1202	150-159	-0.05542524906192625
1202	160-169	0.007136649793700656
1202	170-179	-0.1807840214536185
1202	180-189	-0.09092465052867027
1202	190-199	-0.10764188586833967
1202	200-209	-0.05031711791893656
1202	210-219	0.051467105081371756
1202	220-229	-0.018693467419772958
1202	230-239	0.03821664711586692
1202	240-249	0.03485735218403363
1202	250-259	0.07656018754830285
1202	260-269	0.0846428117078517
1202	270-279	-0.026920666308370755
1202	280-289	-0.022501579574147
1202	290-299	0.01184811264517549
1202	300-309	-0.5847581756515368
1202	310-319	-0.5868233479722846
1202	320-329	-0.3405077361518387
1202	330-339	-0.5125900424721088
1202	340-349	-0.42592098476524853
1202	350-359	-0.35604051559933936
1202	360-369	-0.33918550157519434
1202	370-379	-0.4397390921927027
1202	380-389	-0.33630039901353825
1202	390-399	-0.4733600729465621
1202	400-409	-0.3591000515142113
1202	410-419	-0.2677305405760606
1202	420-429	-0.277552117082422
1202	430-439	-0.23667967792498956
1202	440-449	-0.4562580921628836
1202	450-459	-0.16318853776733988
1202	460-469	0.36063146031011684
1202	470-475	-4.387096774193548
1203	1	0.18633137026679947
1203	2	-0.08691465677155463
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2404	30-39	0.04574123514227324
2404	40-49	0.08143827713764296
2404	50-59	0.10948029800866976
2404	60-69	0.16289567185648224
2404	70-79	0.1416254666723944
2404	80-89	0.08802741284523563
2404	90-99	0.18305315128805688
2404	100-109	0.1456025807003769
2404	110-119	0.28745789167230384
2404	120-129	0.21878214436484456
2404	130-139	0.1564659791013696
2404	140-149	0.29851362210360577
2404	150-159	0.3507365851585007
2404	160-169	0.3965052680490473
2404	170-179	0.5590498515752529
2404	180-189	0.4304075910067908
2404	190-199	0.4758701052980143
2404	200-209	0.41970992889189773
2404	210-219	0.36844890431462574
2404	220-229	0.2663495221213097
2404	230-239	0.31829195635520335
2404	240-249	0.31914290695382874
2404	250-259	0.38326481900448073
2404	260-269	0.2791149646588096
2404	270-279	0.35270241529111246
2404	280-289	0.35403158998951056
2404	290-299	0.4546980772858049
2404	300-309	1.4419983162033994
2404	310-319	1.5241690714765994
2404	320-329	1.4115945950847433
2404	330-339	1.3695776725686457
2404	340-349	1.1960973524762295
2404	350-359	1.084247446088483
2404	360-369	1.1814494781396903
2404	370-379	1.0669903528514126
2404	380-389	0.9030558124478603
2404	390-399	0.9137833868119998
2404	400-409	0.7241805529448087
2404	410-419	0.6328916601410413
2404	420-429	0.6665558964657947
2404	430-439	0.6229454135751169
2404	440-449	0.4731367138239193
2404	450-459	0.44260721614556786
2404	460-469	0.7297578000843359
2404	470-475	-4.387096774193548
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	7.0
18	30.0
19	73.0
20	120.0
21	187.0
22	281.0
23	371.0
24	441.0
25	575.0
26	780.0
27	1031.0
28	1351.0
29	1713.0
30	2684.0
31	5261.0
32	18742.0
33	98632.0
34	2181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.09222073479101592	0.05131637661758144	0.11230105607615648	99.74416183251525
2	0.02677376171352075	0.11453220288561654	0.27740591997620107	99.58128811542466
3	0.0052060092220734796	0.26104417670682734	99.61847389558233	0.11527591848876989
4	0.09519559720362933	99.6779711438346	0.043879220586047894	0.18295403837572513
5	0.17923546035995835	0.032723486538747584	0.2045217908671724	99.58351926223412
6	99.74193068570578	0.07437156031533541	0.02677376171352075	0.15692399226535772
7	99.87208091625762	0.06544697307749517	0.03867321136397441	0.023798899300907332
8	99.81109623679905	0.07809013833110219	0.041648073776587836	0.06916555109326193
9	30.85973523724528	22.172393276810947	25.20229064405771	21.765580841886063
10-19	44.57258664286777	20.501413059645994	14.76803510337647	20.15796519410977
20-29	34.39402052655065	24.126729138777332	30.29213148891864	11.187118845753384
30-39	28.67075710248401	33.89059943477614	10.137513015023055	27.301130447716794
40-49	28.043581734344787	33.80916257623085	15.017997917596313	23.12925777182805
50-59	35.60419455600179	28.71746244236204	10.36813922356091	25.310203778075262
60-69	39.60724378997471	10.708165997322624	18.564033913431505	31.120556299271158
70-79	40.72839506172839	38.64918934999256	14.7085378551242	5.913877733154842
80-89	32.2885616540235	6.915439535921464	42.634389409489806	18.161609400565222
90-99	16.81057563587684	31.13230700580098	32.50319797709356	19.553919381228617
100-109	38.72802320392682	27.007585899152165	24.46906143090882	9.795329466012197
110-119	38.25911051613863	45.40532500371858	11.363453815261044	4.9721106648817495
120-129	29.234493529674253	29.127919083742377	27.083816748475382	14.553770638107988
130-139	23.383385393425556	21.71411572214785	30.798899300907333	24.103599583519262
140-149	25.40465565967574	28.164807377658786	26.08835341365462	20.34218354901086
150-159	21.515915513907483	42.865387475829245	6.507437156031534	29.11125985423174
160-169	31.592741335713225	20.107169418414397	17.010932619366354	31.289156626506028
170-179	30.424959095641828	21.15528781793842	18.694332887103972	29.72542019931578
180-189	36.25717685557043	20.384203480589022	10.860032723486539	32.49858694035401
190-199	21.599583519262232	35.38420348058902	27.941692696712778	15.074520303435964
200-209	46.43345347015941	21.646419917624712	15.916220553958194	16.003906058257684
210-219	42.724230254350736	16.032946601219695	25.075115275918492	16.16770786851108
220-229	35.66920051137999	11.131290639500163	40.273628455217434	12.92588039390241
230-239	36.44610772431037	37.29342354114498	15.35327632996901	10.90719240457564
240-249	39.56611631712033	25.424364123159304	11.841439833407705	23.16807972631266
250-259	21.793706631761356	34.41986032917097	23.242772889877212	20.54366014919046
260-269	29.9238435222371	35.85601665922951	24.290123456790123	9.93001636174327
270-279	24.63282760672319	14.56857057861074	24.45693886657742	36.34166294808865
280-289	26.803361594526255	34.526922504834154	18.446973077495166	20.22274282314443
290-299	36.146660716941845	23.29912241558828	19.379220586047897	21.174996281421983
300-309	29.940874609549304	31.14822251970846	28.669418414398333	10.241484456343894
310-319	26.56091030789826	16.766919529971737	36.12903465714711	20.543135504982896
320-329	49.68153529439764	35.275730044266744	9.75504545670903	5.287689204626589
330-339	29.790340334384485	31.909026001070984	22.60732135419766	15.69331231034688
340-349	33.833320937151356	27.023056898475268	25.858311640014875	13.285310524358499
350-359	37.761428939080865	27.97362169174034	16.742386037883485	17.522563331295306
360-369	33.75388549585956	32.03329606827613	24.561168620292868	9.651649815571437
370-379	29.150138461298152	35.53088732009887	12.715232379191308	22.60374183941167
380-389	34.01051324792714	30.376101142592344	14.529987005345882	21.083398604134633
390-399	40.32394355720523	7.324248532769996	16.32801981597328	36.02378809405149
400-409	35.78236949846469	26.223786627311895	21.20530932374854	16.788534550474875
410-419	31.771405957798805	28.93465999961319	13.253074784166294	26.040859258421715
420-429	35.64038036520475	33.53249715276574	16.933288799192432	13.893833682837073
430-439	36.393658640084276	18.57762359063313	20.4868062043782	24.541911564904392
440-449	27.22120620073833	38.92993761786367	21.039862985028506	12.808993196369498
450-459	16.77989775944965	33.06237053647783	16.74877900457992	33.4089526994926
460-469	32.627601138687304	16.900084198708953	33.576640872459	16.89567379014474
470-479	24.561403508771928	15.789473684210526	22.807017543859647	36.84210526315789
480-484	16.666666666666664	16.666666666666664	33.33333333333333	33.33333333333333
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.8
44	1.05
45	1.5
46	1.0
47	3.5000000000000013
48	33.399999999999935
49	308.39999999999105
50	3608.2999999989097
51	10914.600000004644
52	3565.199999998949
53	1702.0500000002498
54	5383.199999997297
55	4418.049999998174
56	2085.900000000294
57	398.9999999999859
58	294.9999999999918
59	23.799999999999947
60	4.200000000000001
61	4.200000000000001
62	1.25
63	2.1999999999999997
64	3.0000000000000004
65	0.6000000000000001
66	0.2
67	0.0
68	0.0
69	0.0
70	0.2
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.001636174326937379
210-219	0.0
220-229	2.2311468094600627E-4
230-239	7.437156031533541E-5
240-249	0.0
250-259	7.437156031533541E-4
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	7.438151768048675E-5
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-484	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
315-319	2.0
320-324	0.0
325-329	2.0
330-334	0.0
335-339	0.0
340-344	11.0
345-349	1.0
350-354	0.0
355-359	1.0
360-364	1.0
365-369	0.0
370-374	0.0
375-379	0.0
380-384	2.0
385-389	3.0
390-394	0.0
395-399	1.0
400-404	1.0
405-409	0.0
410-414	4.0
415-419	0.0
420-424	3.0
425-429	1.0
430-434	5.0
435-439	16746.0
440-444	33222.0
445-449	203.0
450-454	64.0
455-459	20.0
460-464	5768.0
465-469	78385.0
470-474	10.0
475-479	2.0
480-484	2.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.009965789082255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.40861279544611	3.510337646883831
2	14.676401435465907	1.7640934106797561
3	7.041207771315432	1.2695225345827756
4	4.442519490162108	1.0679756061282166
5	2.7966835787650046	0.8403986315632902
6	2.2150723920306894	0.7987505577867025
7	1.930454151713897	0.8121374386434628
8	1.014725900259869	0.48787743566860037
9	1.0765994307635194	0.5823293172690763
>10	4.999381264694964	5.493083444890674
>50	0.37124118302190323	1.6265060240963853
>100	0.4207400074248237	6.176558084188606
>500	0.19799529761168172	8.813773612970401
>1k	0.3217423586189828	34.397590361445786
>5k	0.08662294270511076	32.35906589320244
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	7968	5.9259259259259265	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	6848	5.092964450394169	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	6256	4.652684813327384	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	6007	4.467499628142199	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	5848	4.349248847240815	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	5543	4.1224155882790425	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	5040	3.748326639892905	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4626	3.4404283801874165	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2736	2.034805890227577	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2446	1.819128365313104	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2196	1.6331994645247656	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2172	1.6153502900490853	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2088	1.5528781793842035	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2075	1.5432098765432098	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1989	1.4792503346720214	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1964	1.4606574445931875	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1774	1.3193514799940502	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1718	1.2777034062174624	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1692	1.2583668005354753	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1688	1.2553919381228618	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1682	1.2509296445039417	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1656	1.2315930388219545	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1521	1.1311914323962518	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1438	1.0694630373345233	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1435	1.067231890525063	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1290	0.9593931280678268	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1285	0.95567455005206	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1265	0.940800237988993	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1219	0.9065893202439387	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1139	0.8470920719916705	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1073	0.798006842183549	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1049	0.7801576677078685	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1035	0.7697456492637216	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	984	0.7318161535029005	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	884	0.657444593187565	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	878	0.652982299568645	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	869	0.6462888591402647	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	817	0.6076156477762903	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	806	0.5994347761416035	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	800	0.5949724825226833	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	741	0.5510932619366354	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	738	0.5488621151271754	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	689	0.512420050572661	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	685	0.5094451881600476	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	655	0.487133720065447	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	634	0.4715156923992265	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	603	0.4484605087014725	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	539	0.4008627100996579	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	529	0.3934255540681244	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	402	0.29897367246764833	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	401	0.298229956864495	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	391	0.2907928008329615	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	373	0.27740591997620107	No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	366	0.27219991075412764	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	331	0.24616986464376023	No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	327	0.24319500223114682	No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	319	0.23724527740591997	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	277	0.2060092220734791	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	275	0.2045217908671724	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	267	0.19857206604194555	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	257	0.19113491001041202	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	256	0.19039119440725866	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	256	0.19039119440725866	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	231	0.1717983043284248	No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	230	0.17105458872527146	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	230	0.17105458872527146	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	223	0.16584857950319798	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	220	0.1636174326937379	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	213	0.15841142347166443	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	212	0.1576677078685111	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	212	0.1576677078685111	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	210	0.15618027666220438	No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	205	0.1524616986464376	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	202	0.15023055183697756	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	198	0.14725568942436412	No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	185	0.13758738658337052	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	168	0.12494422132976349	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	156	0.11601963409192326	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	154	0.11453220288561654	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	152	0.11304477167930983	No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	146	0.1085824780603897	No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	145	0.10783876245723635	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0
320-329	0.0	1.4874312063067083E-4	0.0	0.0	0.0
330-339	0.0	0.002974862412613417	0.0	0.0	0.0
340-349	0.0	0.002974862412613417	0.0	0.0	0.0
350-359	0.0	0.002974862412613417	0.0	0.0	0.0
360-369	0.0	0.002974862412613417	0.0	0.0	0.0
370-379	0.0	0.002974862412613417	0.0	0.0	0.0
380-389	0.0	0.002974862412613417	0.0	0.0	0.0
390-399	0.0	0.002974862412613417	0.0	0.0	0.0
400-409	0.0	0.002974862412613417	0.0	0.0	0.0
410-419	0.0	0.002974862412613417	0.0	0.0	0.0
420-429	0.0	0.002974862412613417	0.0	0.0	0.0
430-439	0.0	0.002974862412613417	0.0	0.0	0.0
440-449	0.0	0.002974862412613417	0.0	0.0	0.0
450-459	0.0	0.002974862412613417	0.0	0.0	0.0
460-469	0.0	0.002974862412613417	0.0	0.0	0.0
470-473	0.0	0.0029748624126134165	0.0	0.0	0.0
>>END_MODULE
