##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IAMS2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	148413
Sequences flagged as poor quality	0
Sequence length	344-487
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.71088786022788	40.0	40.0	40.0	40.0	40.0
2	39.33891235942943	40.0	40.0	40.0	40.0	40.0
3	39.360675951567586	40.0	40.0	40.0	40.0	40.0
4	39.061888109532184	40.0	40.0	40.0	40.0	40.0
5	39.219717949236255	40.0	40.0	40.0	40.0	40.0
6	39.42603410752427	40.0	40.0	40.0	40.0	40.0
7	39.39929790517003	40.0	40.0	40.0	40.0	40.0
8	39.45988558953731	40.0	40.0	40.0	40.0	40.0
9	39.08862431188643	40.0	40.0	40.0	40.0	40.0
10-19	39.56913747448	40.0	40.0	40.0	40.0	40.0
20-29	39.556812408616494	40.0	40.0	40.0	40.0	40.0
30-39	39.59327013132273	40.0	40.0	40.0	40.0	40.0
40-49	39.5752770983674	40.0	40.0	40.0	40.0	40.0
50-59	39.50946884706865	40.0	40.0	40.0	40.0	40.0
60-69	39.4630281713866	40.0	40.0	40.0	40.0	40.0
70-79	39.4930268911753	40.0	40.0	40.0	40.0	40.0
80-89	39.55622485900831	40.0	40.0	40.0	40.0	40.0
90-99	39.477552505508285	40.0	40.0	40.0	40.0	40.0
100-109	39.39861332902104	40.0	40.0	40.0	40.0	40.0
110-119	39.40335280602103	40.0	40.0	40.0	40.0	40.0
120-129	39.37209678397444	40.0	40.0	40.0	40.0	40.0
130-139	39.32489337187444	40.0	40.0	40.0	40.0	40.0
140-149	39.4635887691779	40.0	40.0	40.0	40.0	40.0
150-159	39.55324668324204	40.0	40.0	40.0	40.0	40.0
160-169	39.639854999225136	40.0	40.0	40.0	40.0	40.0
170-179	39.70379009925006	40.0	40.0	40.0	40.0	40.0
180-189	39.74795604158665	40.0	40.0	40.0	40.0	40.0
190-199	39.69260374765013	40.0	40.0	40.0	40.0	40.0
200-209	39.75832575313483	40.0	40.0	40.0	40.0	40.0
210-219	39.71705578352301	40.0	40.0	40.0	40.0	40.0
220-229	39.71987090079709	40.0	40.0	40.0	40.0	40.0
230-239	39.71569067399756	40.0	40.0	40.0	40.0	40.0
240-249	39.76373767796621	40.0	40.0	40.0	40.0	40.0
250-259	39.74686449300263	40.0	40.0	40.0	40.0	40.0
260-269	39.7429632175079	40.0	40.0	40.0	40.0	40.0
270-279	39.71466515736492	40.0	40.0	40.0	40.0	40.0
280-289	39.72233564445163	40.0	40.0	40.0	40.0	40.0
290-299	39.715616556501125	40.0	40.0	40.0	40.0	40.0
300-309	38.916838821397036	40.0	40.0	40.0	38.4	40.0
310-319	38.96325524044389	40.0	40.0	40.0	40.0	40.0
320-329	38.68118022006159	40.0	40.0	40.0	38.4	40.0
330-339	39.07756867659841	40.0	40.0	40.0	38.4	40.0
340-349	38.84180439727773	40.0	40.0	40.0	36.8	40.0
350-359	39.17782820806451	40.0	40.0	40.0	40.0	40.0
360-369	38.605986222859386	40.0	40.0	40.0	36.8	40.0
370-379	38.75221051638256	40.0	40.0	40.0	38.4	40.0
380-389	39.01372892607179	40.0	40.0	40.0	40.0	40.0
390-399	39.09117331841436	40.0	40.0	40.0	40.0	40.0
400-409	39.22670366351257	40.0	40.0	40.0	40.0	40.0
410-419	39.21577479209092	40.0	40.0	40.0	40.0	40.0
420-429	39.2725214858562	40.0	40.0	40.0	40.0	40.0
430-439	39.1829180319857	40.0	40.0	40.0	40.0	40.0
440-449	39.13215948312589	40.0	40.0	40.0	40.0	40.0
450-459	39.20638100163309	40.0	40.0	40.0	38.4	40.0
460-469	39.02427580336901	40.0	40.0	40.0	40.0	40.0
470-479	35.4	NaN	NaN	NaN	NaN	NaN
480-487	40.0	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.03791516409889795
1101	2	-0.2396217550190869
1101	3	0.21721395087599404
1101	4	0.07529378113923002
1101	5	-0.324276939572691
1101	6	0.034413253568246205
1101	7	-0.056022426630960354
1101	8	0.013896010735052755
1101	9	0.1012252353348373
1101	10-19	0.07548482542004109
1101	20-29	0.012763398228720746
1101	30-39	0.044722637023525635
1101	40-49	0.06108488567317494
1101	50-59	0.03360407969424983
1101	60-69	9.336710804745962E-4
1101	70-79	0.095432876264951
1101	80-89	0.0948334191833311
1101	90-99	0.04229158578473147
1101	100-109	0.002004978380448108
1101	110-119	0.06476153191022149
1101	120-129	0.10569306142589596
1101	130-139	0.05573658992408781
1101	140-149	-0.034599623742572305
1101	150-159	-0.0321445505048672
1101	160-169	9.601041254114762E-4
1101	170-179	-0.08025873417467722
1101	180-189	-0.04957080295990579
1101	190-199	0.007161287768013835
1101	200-209	0.006571446192090491
1101	210-219	0.03120676406564371
1101	220-229	0.02380044637547485
1101	230-239	0.034987801183930856
1101	240-249	0.001611986556241618
1101	250-259	0.04910824451039986
1101	260-269	0.05601342254785635
1101	270-279	-0.011934903941082098
1101	280-289	-0.016968277093234008
1101	290-299	-0.017297899827404706
1101	300-309	-0.18271849740839485
1101	310-319	-0.3230612937049955
1101	320-329	-0.06101730379516823
1101	330-339	-0.19151580649172217
1101	340-349	-0.21976472017306037
1101	350-359	-0.13701976641210223
1101	360-369	-0.09942050348450238
1101	370-379	-0.05947560711555866
1101	380-389	-0.08346306279228344
1101	390-399	-0.23233521972192506
1101	400-409	-0.07352392673081454
1101	410-419	-0.23100349932881414
1101	420-429	-0.18003204278821272
1101	430-439	-0.23667223320310882
1101	440-449	-0.28367231532015325
1101	450-459	-0.06982369693673007
1101	460-468	-0.06847891288543195
1102	1	0.11724151965095331
1102	2	0.07557459345026274
1102	3	-0.1415331963656783
1102	4	0.24054128251642481
1102	5	0.39680592813802207
1102	6	-0.08980174603826896
1102	7	-0.16865326002987047
1102	8	-0.2639508453285302
1102	9	0.045633829023358885
1102	10-19	-0.07985002938439578
1102	20-29	-0.10780636646789787
1102	30-39	-0.09329263015204248
1102	40-49	-0.10375181850562143
1102	50-59	-0.17177248440427206
1102	60-69	-0.14521569541044954
1102	70-79	-0.16136888654294523
1102	80-89	-0.05393025438242205
1102	90-99	-0.08815462496524162
1102	100-109	-0.13335820726526038
1102	110-119	-0.2587074222095538
1102	120-129	-0.1189563444134123
1102	130-139	-0.11286969007905157
1102	140-149	-0.12814806161146208
1102	150-159	-0.21610693344340604
1102	160-169	-0.12511764829451266
1102	170-179	-0.05181063470195113
1102	180-189	-0.14979902362567543
1102	190-199	-0.08376654728353827
1102	200-209	-0.06771516748540307
1102	210-219	6.172589771580306E-5
1102	220-229	-0.007695579437061895
1102	230-239	-0.068304872145724
1102	240-249	-0.10181289736600974
1102	250-259	-0.0517558461482821
1102	260-269	-0.10951423937076044
1102	270-279	-0.061393470085718604
1102	280-289	-0.12644887833663887
1102	290-299	-0.08086915976286946
1102	300-309	-0.669691032231718
1102	310-319	-0.5789706350129293
1102	320-329	-0.43102123863423003
1102	330-339	-0.2851634023050664
1102	340-349	-0.6687943888596166
1102	350-359	-0.519475105988711
1102	360-369	-0.5381465912073438
1102	370-379	-0.37367029514785344
1102	380-389	-0.505543688620314
1102	390-399	-0.40644410875100334
1102	400-409	-0.4075722517576281
1102	410-419	-0.3449710723525854
1102	420-429	-0.3621088833660906
1102	430-439	-0.26042512333231826
1102	440-449	-0.3422930251635208
1102	450-459	-0.33625236165874384
1102	460-468	-0.2690000242156074
1103	1	0.012716289048675833
1103	2	-0.02517418571414254
1103	3	-0.20716531790665016
1103	4	-0.42159653625731863
1103	5	0.027060947128795476
1103	6	-0.12018698749242418
1103	7	0.22097235038792462
1103	8	-0.08624059030954356
1103	9	-0.34277637173627795
1103	10-19	-0.09537254701869102
1103	20-29	-0.0727070718395737
1103	30-39	-0.08992420909941501
1103	40-49	-0.154955291104649
1103	50-59	-0.04812191468099769
1103	60-69	-0.12398075238277073
1103	70-79	-0.09190822457875214
1103	80-89	-0.20721077527228005
1103	90-99	-0.16708246001679328
1103	100-109	-0.20265392077583755
1103	110-119	-0.11320552313195975
1103	120-129	-0.17582883491802903
1103	130-139	-0.16567923972636578
1103	140-149	-0.11545137143241391
1103	150-159	-0.12561100289538984
1103	160-169	-0.18414315019360572
1103	170-179	-0.11593651641653935
1103	180-189	-0.11396614245368397
1103	190-199	-0.10420713328462483
1103	200-209	-0.1012083850654335
1103	210-219	-0.060569853049656786
1103	220-229	-0.06452900320267219
1103	230-239	-0.12023759053965222
1103	240-249	-0.07430123160366975
1103	250-259	-0.10573305721720061
1103	260-269	-0.15209264414558987
1103	270-279	-0.13216069743243963
1103	280-289	-0.20390519955205377
1103	290-299	-0.104799707782405
1103	300-309	-0.6281761109077948
1103	310-319	-0.5672332503140751
1103	320-329	-0.5786066971258066
1103	330-339	-0.46530447664037666
1103	340-349	-0.5133782195704129
1103	350-359	-0.4721696257933772
1103	360-369	-0.5704257264656079
1103	370-379	-0.6134040924510415
1103	380-389	-0.40155064816794805
1103	390-399	-0.3957229256279078
1103	400-409	-0.39532866759059715
1103	410-419	-0.34220639205680925
1103	420-429	-0.3041654296905776
1103	430-439	-0.19709162077526798
1103	440-449	-0.2364468138436564
1103	450-459	-0.3882702988784814
1103	460-468	-8.773168227591555E-4
1104	1	-0.05401692329252228
1104	2	-0.1492032238266674
1104	3	-0.08780052661808213
1104	4	-0.14239508435169057
1104	5	0.001071836420990735
1104	6	-0.11029588041438387
1104	7	0.09309576236614703
1104	8	-0.005187958730594744
1104	9	-0.18246784179072506
1104	10-19	-0.060978717617594214
1104	20-29	-0.04485044752016165
1104	30-39	-0.09432711291066909
1104	40-49	-0.12434549074168189
1104	50-59	-0.1527607531564925
1104	60-69	-0.15232376508694045
1104	70-79	-0.16109515742811453
1104	80-89	-0.06965723625231135
1104	90-99	-0.07218590829265281
1104	100-109	-0.10353595344371769
1104	110-119	-0.03332349046408467
1104	120-129	-0.0671682178581321
1104	130-139	-0.13648504734887013
1104	140-149	-0.022102914082140046
1104	150-159	-0.08699122068122023
1104	160-169	-0.05821665291591671
1104	170-179	-0.13945194291381569
1104	180-189	-0.11436087929578065
1104	190-199	-0.052321470852689345
1104	200-209	-0.06239078070972681
1104	210-219	-0.021662412033329304
1104	220-229	-0.017332088502122645
1104	230-239	-0.046069713951631286
1104	240-249	-0.07422046935321447
1104	250-259	-0.0741314238233528
1104	260-269	-0.14660897808388995
1104	270-279	-0.1182260331856142
1104	280-289	-0.16865293094951284
1104	290-299	-0.09787502538676307
1104	300-309	-0.4354428985665919
1104	310-319	-0.45925502889846115
1104	320-329	-0.7274360982147385
1104	330-339	-0.3694968541358534
1104	340-349	-0.4407176025105244
1104	350-359	-0.25045093250843564
1104	360-369	-0.573578176556353
1104	370-379	-0.4446164336488607
1104	380-389	-0.589563352342175
1104	390-399	-0.36251421419414953
1104	400-409	-0.22205643528570107
1104	410-419	-0.3390648312582698
1104	420-429	-0.16966089248550276
1104	430-439	-0.38233710733655357
1104	440-449	-0.11655995857126555
1104	450-459	-0.40281575342392983
1104	460-468	-0.3622306867132252
1201	1	-0.12372968847441257
1201	2	0.026892132300766036
1201	3	-0.10054019272583048
1201	4	0.021051733465618838
1201	5	-0.07751677624941777
1201	6	-0.03001229070817857
1201	7	-0.13548920519235708
1201	8	-0.13098077056991997
1201	9	-0.02665753341425159
1201	10-19	0.02508055190197922
1201	20-29	0.05119278350702672
1201	30-39	0.01630567851602649
1201	40-49	-0.0017348615953594049
1201	50-59	-0.09092507480117717
1201	60-69	0.08637821056370854
1201	70-79	-0.03351988586728538
1201	80-89	0.015055164418392053
1201	90-99	-0.05582664606330212
1201	100-109	-0.12231921508983845
1201	110-119	-0.22188695186235208
1201	120-129	-0.09210078328789706
1201	130-139	-0.11342242432634464
1201	140-149	-0.17737192334071494
1201	150-159	-0.161428962169893
1201	160-169	-0.16825620238009265
1201	170-179	-0.07792427651446587
1201	180-189	-0.09689259176936105
1201	190-199	-0.050978247115708086
1201	200-209	-0.08786555653534123
1201	210-219	-0.08146047283378977
1201	220-229	-0.05411217890089404
1201	230-239	-0.08851977591853455
1201	240-249	-0.10772907181730318
1201	250-259	-0.03705607936029054
1201	260-269	-0.12124720072732487
1201	270-279	-0.11868167217372871
1201	280-289	-0.07526516394506189
1201	290-299	-0.09668398507182019
1201	300-309	-0.21636438285616322
1201	310-319	-0.12406511856821822
1201	320-329	-0.1459550892477779
1201	330-339	-0.07535454896707705
1201	340-349	-0.06966708125951726
1201	350-359	-0.02593573239179392
1201	360-369	-0.10256592898036132
1201	370-379	-0.07313214408117119
1201	380-389	-0.08943759598145817
1201	390-399	-0.20244641511271766
1201	400-409	-0.13217344337784454
1201	410-419	-0.15590707081501876
1201	420-429	-0.023477324649974207
1201	430-439	-0.017037687848251437
1201	440-449	-0.11677057669083979
1201	450-459	-0.055577991186176234
1201	460-468	0.058869178312576764
1202	1	0.010872143801833545
1202	2	-0.02878087686061548
1202	3	-0.020906647976005388
1202	4	0.1866613176798495
1202	5	-0.06976483826492341
1202	6	0.3209390834990131
1202	7	0.25281241566739965
1202	8	0.045199638168632816
1202	9	-0.08937775892518118
1202	10-19	0.11281839545377181
1202	20-29	0.03970582085727159
1202	30-39	0.07973062566190947
1202	40-49	0.10841312994797647
1202	50-59	0.05001925218966363
1202	60-69	0.07108575108520654
1202	70-79	0.12095509651467751
1202	80-89	0.06410379021119894
1202	90-99	0.1480493229289479
1202	100-109	0.1470253937896473
1202	110-119	0.07057252664363034
1202	120-129	0.06667300106729357
1202	130-139	0.0399250034396843
1202	140-149	0.09193744945702775
1202	150-159	0.06587900118458379
1202	160-169	0.03960144384964792
1202	170-179	-0.014745981940819775
1202	180-189	-0.006124445187261074
1202	190-199	0.0346806676129745
1202	200-209	-0.0017485727439350285
1202	210-219	0.007968702641903747
1202	220-229	0.03003158431263131
1202	230-239	0.04439072443383196
1202	240-249	0.0034760021784592254
1202	250-259	0.03510741605901302
1202	260-269	-0.01176869473719222
1202	270-279	0.010669983922106496
1202	280-289	0.06574963520926502
1202	290-299	-0.01409060945518803
1202	300-309	0.027962607982452425
1202	310-319	-0.01603129192974251
1202	320-329	-0.22706854943100296
1202	330-339	-0.055129038043887135
1202	340-349	-0.05571359288742883
1202	350-359	0.1345998560507624
1202	360-369	-0.04484922707759864
1202	370-379	-0.1303555408394459
1202	380-389	-0.12481462205614235
1202	390-399	0.09980869411914028
1202	400-409	-0.03386477530173693
1202	410-419	0.09025219627447711
1202	420-429	0.047206255335936476
1202	430-439	0.0037941625182185135
1202	440-449	-0.12180110791352661
1202	450-459	0.1814776002811911
1202	460-468	0.008853052625937607
1203	1	-0.016089185440606002
1203	2	-0.18780677100145482
1203	3	0.13239793055868176
1203	4	0.13491750218486942
1203	5	-0.1580895006924763
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2404	140-149	0.09602372887442101
2404	150-159	0.12426013234180999
2404	160-169	0.10164301926592856
2404	170-179	0.1125543969655709
2404	180-189	0.10867723729614909
2404	190-199	0.030479673418234654
2404	200-209	0.10150863169170066
2404	210-219	0.07154154652550915
2404	220-229	0.1454346503257682
2404	230-239	0.1161674130005963
2404	240-249	0.1300376874428295
2404	250-259	0.08758768859351562
2404	260-269	0.08793756590868895
2404	270-279	0.08318884233626278
2404	280-289	0.07586539284822891
2404	290-299	0.028746669371237488
2404	300-309	0.3294712811148699
2404	310-319	0.35982646174634425
2404	320-329	0.32529700832615305
2404	330-339	0.20122372727747972
2404	340-349	0.2511797223696277
2404	350-359	0.1459809524459459
2404	360-369	0.27579943284522557
2404	370-379	0.23021733780622355
2404	380-389	0.17833134380751403
2404	390-399	0.20021193394254766
2404	400-409	0.0509720168559511
2404	410-419	0.08769023291368683
2404	420-429	0.1417972797736553
2404	430-439	0.12366261504033815
2404	440-449	0.11781192321863188
2404	450-459	0.22225601691099683
2404	460-468	0.26484443882404207
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	6.0
23	14.0
24	41.0
25	57.0
26	97.0
27	144.0
28	182.0
29	258.0
30	347.0
31	419.0
32	580.0
33	731.0
34	1030.0
35	1377.0
36	2003.0
37	3143.0
38	6551.0
39	127307.0
40	4126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.008085545066806815	0.009433135911274619	0.09028858657934279	99.89219273244258
2	0.005390399698137617	0.0060641996604048195	0.27154138479368245	99.71700401584778
3	0.0013475908444678028	0.2068551946258077	99.65501674381623	0.13678047071348198
4	0.06737954222339013	99.49532722874682	0.04042772533403408	0.3968655036957679
5	0.1489087883136922	0.012802113022444125	0.09365756369051229	99.74463153497335
6	99.91981834475416	0.06670574680115623	0.008759340489040717	0.004716567955637309
7	99.916449367643	0.0518822475120104	0.022909044355952646	0.008759340489040717
8	99.86793609724216	0.07546508729019695	0.037732543645098475	0.018866271822549237
9	28.77443350649876	21.281828411257774	26.74698308099695	23.196755001246522
10-19	49.697398475874756	15.30890151132313	14.785497227331836	20.208202785470274
20-29	40.51080430959552	29.111533356242376	30.009298376826827	0.3683639573352739
30-39	34.23696037409122	29.483603188399936	10.085167741370366	26.19426869613848
40-49	27.51787242357475	34.549062413669965	10.68248738318072	27.250577779574563
50-59	30.938260125460708	23.869404971262625	13.500367218505119	31.691967684771548
60-69	36.22014244035226	10.747441261884067	21.22031088920782	31.81210540855585
70-79	38.51663937795207	38.84956169607784	8.204267820204429	14.429531105765667
80-89	37.01576007492605	3.0257457230835576	47.41673573069745	12.541758471292946
90-99	10.80552242728063	36.79037550618881	36.03006475174008	16.37403731479048
100-109	44.56348163570576	27.95119025961338	5.601328724572645	21.883999380108214
110-119	39.37849110253145	41.84714277051202	12.689454427846616	6.084911699109917
120-129	22.379441154076797	34.13784506748061	27.683558717902066	15.799155060540517
130-139	17.987575212414004	26.453882072325204	23.75499450856731	31.80354820669348
140-149	20.167168644256233	25.850565651256964	24.002007910358255	29.980257794128544
150-159	33.97896410691786	33.67184815346364	6.6882954997203745	25.66089223989812
160-169	31.146462910930982	19.243395120373552	14.309797659234702	35.30034430946076
170-179	36.63560469770169	18.14207650273224	16.95788104815616	28.264437751409915
180-189	32.742751645745315	21.13999447487754	16.92708859736007	29.190165282017073
190-199	24.699184033743673	31.034612870840157	24.761712248926983	19.50449084648919
200-209	44.99612567632216	23.296544103279363	19.028926037476502	12.678404182921982
210-219	44.87187780046222	22.827380350777897	21.844717106991975	10.456024741767903
220-229	34.86338797814207	16.132885933172968	29.242586565866873	19.761139522818084
230-239	33.02150081192348	37.86036263669625	13.98179404769124	15.13634250368903
240-249	35.452419936258956	25.65920775134254	15.118486924999832	23.769885387398677
250-259	24.101729632848876	19.80702499107221	29.18551609360366	26.905729282475257
260-269	31.526955185866466	34.122010875058116	19.03445115993882	15.316582779136597
270-279	23.557639829395	17.98562120568953	28.410583978492447	30.046154986423023
280-289	30.812664658756304	25.4306563441208	22.447224973553528	21.309454023569362
290-299	35.082775767621435	22.94691165868219	23.23866507650947	18.731647497186906
300-309	38.81688261809949	34.15064718050306	14.81716561217683	12.215304589220622
310-319	23.73350043459805	17.785773483454953	37.200447400160364	21.280278681786633
320-329	52.405517036917246	21.607271600196746	16.489188952450256	9.498022410435745
330-339	30.11050244924636	32.20358054887375	27.39429834313706	10.291618658742832
340-349	40.034027127369264	28.048460019809852	21.587887690265546	10.329625162555336
350-359	29.735446241587404	33.79781737421871	15.688128165252103	20.778608218941784
360-369	43.46882230476156	25.052081332473342	14.984663724096656	16.494432638668442
370-379	31.951212936188572	25.36644221112302	21.628751878888373	21.05359297380004
380-389	34.56379706751272	28.084163584609463	16.692448774490476	20.659590573387337
390-399	40.50782908794327	9.614713158125333	9.183119097851804	40.694338656079594
400-409	42.720701786079005	35.39964503558744	9.9474968133417	11.932156364991855
410-419	27.37284953611795	34.17074553459179	10.626852511596208	27.829552417694053
420-429	36.761059751577754	35.41763007923465	19.66932310127393	8.151987067913677
430-439	40.73738568774864	13.544526299743282	18.719621843808216	26.99846616869986
440-449	19.834451410455905	45.246547304670095	19.916192640706615	15.00280864416738
450-459	16.79522627077353	33.31073267233738	16.473093472811698	33.420947584077396
460-469	33.02491033069253	16.810039020929405	33.36388002049584	16.80117062788223
470-479	21.052631578947366	26.31578947368421	26.31578947368421	26.31578947368421
480-487	50.0	12.5	25.0	12.5
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.2
48	3.0000000000000004
49	296.5999999999917
50	747.600000000033
51	101.0000000000009
52	301.9999999999914
53	3267.749999999221
54	10477.350000003056
55	14760.950000018638
56	3559.3999999989537
57	2006.200000000319
58	241.7999999999948
59	2.8000000000000003
60	0.0
61	0.4
62	0.0
63	5.200000000000002
64	12.599999999999987
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	6.737954222339014E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	0.0
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-487	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
340-344	4.0
345-349	0.0
350-354	1.0
355-359	0.0
360-364	49.0
365-369	0.0
370-374	0.0
375-379	0.0
380-384	0.0
385-389	2.0
390-394	0.0
395-399	1.0
400-404	4.0
405-409	0.0
410-414	7.0
415-419	0.0
420-424	1.0
425-429	0.0
430-434	2.0
435-439	31382.0
440-444	48812.0
445-449	6.0
450-454	3.0
455-459	1.0
460-464	3062.0
465-469	65074.0
470-474	0.0
475-479	1.0
480-484	0.0
485-488	1.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	3.3487632485024896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.72233400402414	1.7990337773645166
2	15.01006036217304	1.0053027699729808
3	7.665995975855131	0.7701481676133493
4	4.466800804828973	0.5983303349437044
5	2.977867203219316	0.49860861245308696
6	2.27364185110664	0.45683329627458513
7	2.0120724346076457	0.4716567955637309
8	1.529175050301811	0.409667616718212
9	1.5492957746478873	0.4669402276080936
>10	6.680080482897384	4.040751147136707
>50	0.5432595573440644	1.2202435096655952
>100	0.7847082494969818	5.807442744233995
>500	0.18108651911468815	4.922075559418649
>1k	0.46277665995975853	40.445917810434395
>5k	0.14084507042253522	37.0870476305984
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9230	6.21913174721891	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8510	5.733999043210501	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8008	5.395753741249082	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	7843	5.284577496580488	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	7535	5.077048506532447	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	7108	4.789337861238571	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	6808	4.5871992345684	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	4980	3.3555012027248288	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4103	2.764582617425697	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3838	2.5860268305337133	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3603	2.4276849063087464	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3497	2.356262591551953	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3324	2.239695983505488	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	3268	2.2019634398603896	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	3235	2.179728190926671	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3045	2.0517070607022294	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3035	2.0449691064798903	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	3028	2.0402525385242534	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2781	1.8738250692324796	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2701	1.8199214354537676	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2598	1.7505205069636758	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2445	1.6474298073618887	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2211	1.4897616785591559	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1827	1.2310242364213377	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1244	0.8382015052589733	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1130	0.7613888271243086	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1058	0.7128755567234677	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1039	0.7000734437010235	No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1025	0.6906403077897489	No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1012	0.6818809673007081	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	956	0.6441484236556096	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	935	0.6299987197886978	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	864	0.5821592448100907	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	859	0.5787902676989212	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	792	0.5336459744092499	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	779	0.5248866339202092	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	741	0.49928240787532085	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	727	0.48984927196404626	No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	652	0.43931461529650373	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	451	0.30388173542748953	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	437	0.2944485995162149	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	395	0.26614919178239105	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	377	0.2540208741821808	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	376	0.2533470787599469	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	353	0.2378497840485672	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	328	0.22100489849271962	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	321	0.21628833053708232	No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	270	0.18192476400315336	No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	266	0.17922958231421776	No Hit
CCTACGGGCGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	244	0.16440608302507193	No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	230	0.1549729471137973	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	229	0.1542991516915634	No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	228	0.1536253562693295	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	227	0.1529515608470956	No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	207	0.13947565240241758	No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	206	0.13880185698018369	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	206	0.13880185698018369	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	199	0.13408528902454636	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	190	0.12802113022444125	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	188	0.12667353937997344	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	181	0.12195697142433615	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	178	0.11993558515763444	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	177	0.11926178973540053	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	174	0.11724040346869882	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	171	0.11521901720199713	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	168	0.11319763093529543	No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	166	0.11185004009082763	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	159	0.10713347213519031	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	150	0.1010693133350852	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0
320-329	0.0	0.0	0.0	0.0	0.0
330-339	0.0	0.0	0.0	0.0	0.0
340-349	0.0	0.0	0.0	0.0	0.0
350-359	0.0	0.0	0.0	0.0	0.0
360-369	0.0	0.0	0.0	0.0	0.0
370-379	0.0	0.0	0.0	0.0	0.0
380-389	0.0	0.0	0.0	0.0	0.0
390-399	0.0	0.0	0.0	0.0	0.0
400-409	0.0	0.0	0.0	0.0	0.0
410-419	0.0	0.0	0.0	0.0	0.0
420-429	0.0	0.0	0.0	0.0	0.0
430-439	0.0	0.0	0.0	0.0	0.0
440-449	0.0	0.0	0.0	0.0	0.0
450-459	0.0	0.0	0.0	0.0	0.0
460-469	0.0	0.0	0.0	0.0	0.0
470-476	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
