##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IAMS1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	148021
Sequences flagged as poor quality	0
Sequence length	321-497
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.75849372724141	40.0	40.0	40.0	40.0	40.0
2	39.08496767350579	40.0	40.0	40.0	40.0	40.0
3	39.21297653711298	40.0	40.0	40.0	40.0	40.0
4	39.0988035481452	40.0	40.0	40.0	40.0	40.0
5	39.20151870342722	40.0	40.0	40.0	40.0	40.0
6	39.39303206977388	40.0	40.0	40.0	40.0	40.0
7	39.35255132717655	40.0	40.0	40.0	40.0	40.0
8	39.46721073361212	40.0	40.0	40.0	40.0	40.0
9	39.03446132643341	40.0	40.0	40.0	40.0	40.0
10-19	39.543999837860845	40.0	40.0	40.0	40.0	40.0
20-29	39.54170286648516	40.0	40.0	40.0	40.0	40.0
30-39	39.59006357206072	40.0	40.0	40.0	40.0	40.0
40-49	39.53103951466346	40.0	40.0	40.0	40.0	40.0
50-59	39.5262023631782	40.0	40.0	40.0	40.0	40.0
60-69	39.43609622958905	40.0	40.0	40.0	40.0	40.0
70-79	39.450350963714605	40.0	40.0	40.0	40.0	40.0
80-89	39.56233237175806	40.0	40.0	40.0	40.0	40.0
90-99	39.42131184088744	40.0	40.0	40.0	38.4	40.0
100-109	39.37685328433128	40.0	40.0	40.0	40.0	40.0
110-119	39.38713831145581	40.0	40.0	40.0	40.0	40.0
120-129	39.32409320299146	40.0	40.0	40.0	40.0	40.0
130-139	39.26240600995805	40.0	40.0	40.0	40.0	40.0
140-149	39.32532275825728	40.0	40.0	40.0	38.4	40.0
150-159	39.411726714452676	40.0	40.0	40.0	40.0	40.0
160-169	39.574354990170306	40.0	40.0	40.0	40.0	40.0
170-179	39.70920342383852	40.0	40.0	40.0	40.0	40.0
180-189	39.752590510805895	40.0	40.0	40.0	40.0	40.0
190-199	39.69132623073753	40.0	40.0	40.0	40.0	40.0
200-209	39.76451719688423	40.0	40.0	40.0	40.0	40.0
210-219	39.68645394910182	40.0	40.0	40.0	40.0	40.0
220-229	39.72139763952413	40.0	40.0	40.0	40.0	40.0
230-239	39.71656319035813	40.0	40.0	40.0	40.0	40.0
240-249	39.766983063214006	40.0	40.0	40.0	40.0	40.0
250-259	39.724456664932674	40.0	40.0	40.0	40.0	40.0
260-269	39.74326210470135	40.0	40.0	40.0	40.0	40.0
270-279	39.68505685004155	40.0	40.0	40.0	40.0	40.0
280-289	39.727661615581574	40.0	40.0	40.0	40.0	40.0
290-299	39.74145695543199	40.0	40.0	40.0	40.0	40.0
300-309	38.902334128265586	40.0	40.0	40.0	38.4	40.0
310-319	38.90473649009262	40.0	40.0	40.0	40.0	40.0
320-329	38.54870088269674	40.0	40.0	40.0	36.8	40.0
330-339	38.93837319281179	40.0	40.0	40.0	38.4	40.0
340-349	38.92461289014998	40.0	40.0	40.0	38.4	40.0
350-359	39.108626703314016	40.0	40.0	40.0	40.0	40.0
360-369	38.237858653280995	40.0	40.0	40.0	35.2	40.0
370-379	38.647253393145476	40.0	40.0	40.0	38.4	40.0
380-389	39.065048318303674	40.0	40.0	40.0	40.0	40.0
390-399	39.16224031348174	40.0	40.0	40.0	40.0	40.0
400-409	39.14035149473662	40.0	40.0	40.0	40.0	40.0
410-419	39.21583904225275	40.0	40.0	40.0	40.0	40.0
420-429	39.31743572097725	40.0	40.0	40.0	40.0	40.0
430-439	39.18774748631874	40.0	40.0	40.0	40.0	40.0
440-449	39.02913211773446	40.0	40.0	40.0	40.0	40.0
450-459	39.13935270157528	40.0	40.0	40.0	38.4	40.0
460-469	39.19057228412275	40.0	40.0	40.0	40.0	40.0
470-479	40.0	NaN	NaN	NaN	NaN	NaN
480-489	40.0	NaN	NaN	NaN	NaN	NaN
490-497	40.0	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.09355610514364088
1101	2	0.27007899226902765
1101	3	0.4309930599469993
1101	4	0.13634469411503858
1101	5	0.14065851004282592
1101	6	0.05966488595424835
1101	7	0.1519217953046592
1101	8	0.13461825318717047
1101	9	0.5341677339320583
1101	10-19	0.13951414729026368
1101	20-29	0.05615363564818665
1101	30-39	0.09601976241498278
1101	40-49	0.10100921069376056
1101	50-59	0.08235446840754435
1101	60-69	0.13200257082395694
1101	70-79	0.16417460736647627
1101	80-89	0.1416409846857789
1101	90-99	0.16785681130911456
1101	100-109	0.19800406940960613
1101	110-119	0.06815531047119805
1101	120-129	0.070872561073827
1101	130-139	0.16289056086145592
1101	140-149	0.008737679821472
1101	150-159	-0.01431946620759561
1101	160-169	-0.021861779257264402
1101	170-179	-0.03381539289080138
1101	180-189	-0.013623888760342595
1101	190-199	0.02851416736071144
1101	200-209	-0.02760772861952887
1101	210-219	0.136484036149362
1101	220-229	0.11424666276711548
1101	230-239	0.010085642744556367
1101	240-249	-0.016720102589893315
1101	250-259	0.05765047578258731
1101	260-269	0.020324553468569206
1101	270-279	0.03302298629378697
1101	280-289	0.024138928483786515
1101	290-299	0.0414955841519955
1101	300-309	-0.0895205389225282
1101	310-319	-0.023841116272372176
1101	320-329	0.04069404498894613
1101	330-339	-0.014859190354115981
1101	340-349	-0.09127596704067997
1101	350-359	-0.03758760780884529
1101	360-369	-0.06295555143992715
1101	370-379	-0.036979853523945394
1101	380-389	-0.08023142765014057
1101	390-399	-0.02276852388430939
1101	400-409	-0.0862201117018273
1101	410-419	-0.03365534117761371
1101	420-429	0.041208003053434084
1101	430-439	0.01468562857066047
1101	440-449	-0.07355951279497219
1101	450-459	0.06009963994794987
1101	460-468	-0.18343790395304183
1102	1	-0.0018013245752328544
1102	2	-0.23135072660647182
1102	3	0.06437458605141444
1102	4	0.1395896740347169
1102	5	0.2953894337376042
1102	6	-0.026086118061819263
1102	7	-0.08608623682385286
1102	8	2.2467888997823593E-4
1102	9	0.20803118774731644
1102	10-19	-0.02083605351295148
1102	20-29	-0.0237949587293258
1102	30-39	-0.01549830987417522
1102	40-49	-0.08867432344278825
1102	50-59	-0.17443268018683256
1102	60-69	-0.07421750949733052
1102	70-79	-0.15192177817569075
1102	80-89	-0.06357347314555284
1102	90-99	-0.08334318869089685
1102	100-109	-0.02987384223698797
1102	110-119	-0.028869589127204165
1102	120-129	-0.14592422607476863
1102	130-139	-0.07961867608632645
1102	140-149	-0.09904866556004777
1102	150-159	-0.08154516901884534
1102	160-169	-0.1232738274500349
1102	170-179	-0.14007884670606785
1102	180-189	-0.15655561566798326
1102	190-199	-0.15112599328186604
1102	200-209	-0.11052660412153159
1102	210-219	-0.039586646581561524
1102	220-229	-0.09706739345777748
1102	230-239	-0.08437379500644937
1102	240-249	-0.12010805439712158
1102	250-259	-0.10309651216921623
1102	260-269	-0.07435014532660489
1102	270-279	-0.1446364514572096
1102	280-289	-0.1152875775403146
1102	290-299	-0.12964979737411397
1102	300-309	-0.412392828079156
1102	310-319	-0.37854955000730683
1102	320-329	-0.4112513365371555
1102	330-339	-0.3690150136472923
1102	340-349	-0.36991371804469964
1102	350-359	-0.4090767644353548
1102	360-369	-0.4956752301547809
1102	370-379	-0.327476239066101
1102	380-389	-0.36658002202764095
1102	390-399	-0.39538860420559274
1102	400-409	-0.1960835655170854
1102	410-419	-0.28880393555512995
1102	420-429	-0.18021850297066777
1102	430-439	-0.2759305911469241
1102	440-449	-0.1814327722485416
1102	450-459	-0.2110983420072614
1102	460-468	-0.3318361580708711
1103	1	0.06567408526083085
1103	2	-0.18571138234418072
1103	3	0.2026040942481373
1103	4	-0.06880376858823212
1103	5	0.175848450131042
1103	6	0.21207781636441325
1103	7	0.1908973697335199
1103	8	0.21655254774243815
1103	9	0.13117872873092296
1103	10-19	0.10278361861819718
1103	20-29	0.048513237991990366
1103	30-39	0.018141034388122534
1103	40-49	-0.031349733278858594
1103	50-59	0.053600106698418415
1103	60-69	0.008130031486288658
1103	70-79	0.01138969723415073
1103	80-89	-0.05213740757177732
1103	90-99	-0.030503844428594107
1103	100-109	-0.03950662912223635
1103	110-119	-0.12413516289769433
1103	120-129	-0.05291439000919951
1103	130-139	-0.04064818428305017
1103	140-149	-0.0480945671993922
1103	150-159	-0.07730254606801168
1103	160-169	-0.06421808974511833
1103	170-179	-0.08246573195196305
1103	180-189	-0.04122774681552244
1103	190-199	-0.0454604195113717
1103	200-209	-0.007326604121537628
1103	210-219	0.06635433702499682
1103	220-229	-0.027382147556131997
1103	230-239	-0.06092133599005933
1103	240-249	-0.042806415052858426
1103	250-259	-0.024588315447907405
1103	260-269	0.03771542844388165
1103	270-279	0.03619633542803058
1103	280-289	-5.203644255615814E-4
1103	290-299	-0.03173504327575927
1103	300-309	-0.41578954939063806
1103	310-319	-0.15914627131878945
1103	320-329	-0.48663494309452915
1103	330-339	-0.32430681692596863
1103	340-349	-0.1900579803397946
1103	350-359	-0.12954889558289295
1103	360-369	-0.42976047605642265
1103	370-379	-0.1938631243120028
1103	380-389	-0.2083636285850119
1103	390-399	-0.10565745666460913
1103	400-409	-0.12503438518921683
1103	410-419	-0.13309901752235476
1103	420-429	-0.08591030624935314
1103	430-439	0.02809885078007568
1103	440-449	-0.16519612466432676
1103	450-459	-0.04487234884756219
1103	460-468	0.14521512815601767
1104	1	-0.11902647670505218
1104	2	-0.19891259273223483
1104	3	-0.2946193287558856
1104	4	-0.05038192839936073
1104	5	-0.14212780764170674
1104	6	0.01339461216130644
1104	7	-0.02351422871026898
1104	8	-0.12097613246904615
1104	9	-0.04662195626890764
1104	10-19	0.05034122843430566
1104	20-29	-0.042962504368269094
1104	30-39	-0.015141311902574728
1104	40-49	-0.05699399889918766
1104	50-59	-0.03775925219493814
1104	60-69	-0.037216292458779776
1104	70-79	-0.09927269095459224
1104	80-89	0.004760806773312254
1104	90-99	-0.05770099802152373
1104	100-109	-0.07005396394083618
1104	110-119	-0.06798845322455804
1104	120-129	-0.08451083865082865
1104	130-139	-0.012086018885518968
1104	140-149	-0.10620566353165373
1104	150-159	-0.11567417510403288
1104	160-169	-0.12375820878877164
1104	170-179	-0.15769101709145872
1104	180-189	-0.13641362783836541
1104	190-199	-0.09572477624332265
1104	200-209	-0.13014607673815703
1104	210-219	-0.029665348407114323
1104	220-229	-0.0570665820987486
1104	230-239	-0.13357704044255314
1104	240-249	-0.07932671565473015
1104	250-259	-0.06094235395419645
1104	260-269	-0.12400937453553951
1104	270-279	-0.10455450419554779
1104	280-289	-0.10084457551191406
1104	290-299	-0.07994107526458549
1104	300-309	-0.30880986661871646
1104	310-319	-0.3005438704941312
1104	320-329	-0.5204992067197125
1104	330-339	-0.26091440512801967
1104	340-349	-0.2196792352860797
1104	350-359	-0.26362929993231177
1104	360-369	-0.37106995632111506
1104	370-379	-0.36330747638862704
1104	380-389	-0.3298546670580649
1104	390-399	-0.1611557441650291
1104	400-409	-0.18900040121688733
1104	410-419	-0.19605748525087563
1104	420-429	-0.2225178944513999
1104	430-439	-0.057237591910073604
1104	440-449	-0.22643634365908838
1104	450-459	-0.1600789975447796
1104	460-468	-0.48692838690023876
1201	1	0.18478435872194865
1201	2	0.05364710419613061
1201	3	0.11693857737462565
1201	4	0.3402144028850387
1201	5	0.016448002067321
1201	6	0.0926297170791841
1201	7	-0.01819903508850018
1201	8	0.19782988496373122
1201	9	-0.10192108990995052
1201	10-19	0.05543466232219174
1201	20-29	0.06726968335311767
1201	30-39	0.0026188267852660374
1201	40-49	0.10442068740319144
1201	50-59	0.004873176646150057
1201	60-69	0.05290439939638247
1201	70-79	0.08628212637962207
1201	80-89	0.03368813206052579
1201	90-99	-0.007414338365506978
1201	100-109	-0.024836098202278833
1201	110-119	0.08958767768407938
1201	120-129	0.05388835527013214
1201	130-139	0.13124292911974322
1201	140-149	0.0786372346568669
1201	150-159	0.10029604573169593
1201	160-169	0.016883222441506973
1201	170-179	-0.0298306905238519
1201	180-189	-0.050677090722210494
1201	190-199	-0.02382534252264179
1201	200-209	-0.008519662689856489
1201	210-219	0.058943505262256224
1201	220-229	0.05232696662898917
1201	230-239	-0.055316094355696066
1201	240-249	-0.05568202402835709
1201	250-259	-0.03625486357919527
1201	260-269	-0.02947812797303584
1201	270-279	-0.057541440611231565
1201	280-289	-0.07522076626048602
1201	290-299	-0.0029842876127261775
1201	300-309	-0.2681134354544312
1201	310-319	-0.18337905109191155
1201	320-329	-0.15779884196014393
1201	330-339	-0.04795818067548652
1201	340-349	0.06880905852796104
1201	350-359	-0.04957307679978129
1201	360-369	-0.05805961193352971
1201	370-379	-0.02853827811166809
1201	380-389	-0.1398312150862111
1201	390-399	-0.10013133739431623
1201	400-409	-0.03971914035439994
1201	410-419	-0.05724645182410626
1201	420-429	-0.044268828350276124
1201	430-439	-0.03906040121949417
1201	440-449	0.00851198666537556
1201	450-459	0.02661574639053299
1201	460-468	0.11230509410313516
1202	1	0.0716589928850837
1202	2	0.06344292418717856
1202	3	0.14145395113078507
1202	4	0.12219284863789426
1202	5	0.0694211797693498
1202	6	-0.12411786409356296
1202	7	-0.17548306222067822
1202	8	-0.06694992428462143
1202	9	-0.21425452653839727
1202	10-19	-0.07025827573517063
1202	20-29	-0.10321718095153898
1202	30-39	-0.05492688130274814
1202	40-49	-0.0857473393158017
1202	50-59	-0.06760728336143274
1202	60-69	-0.017461953941776187
1202	70-79	-0.10833447658838224
1202	80-89	-0.11117982235190027
1202	90-99	-0.0813495378972462
1202	100-109	-0.06512463588779127
1202	110-119	-0.06664101769862896
1202	120-129	-0.14499089274143984
1202	130-139	-0.016799628467282446
1202	140-149	-0.04300739571878154
1202	150-159	0.0014770532033807626
1202	160-169	-0.13479446237066384
1202	170-179	-0.01198043400765414
1202	180-189	-0.015603234715598546
1202	190-199	0.0081565464006772
1202	200-209	0.0021972054022683096
1202	210-219	0.00849589310097798
1202	220-229	-0.026965806156184158
1202	230-239	-0.004376969609623416
1202	240-249	-0.05656519725425824
1202	250-259	-0.0036996867723857463
1202	260-269	-0.05787395485042168
1202	270-279	0.03363973901898021
1202	280-289	5.2104999362256876E-5
1202	290-299	-0.008738686263008333
1202	300-309	-0.24767219315852174
1202	310-319	-0.33348923254699514
1202	320-329	-0.2773973682831965
1202	330-339	-0.19905310888538708
1202	340-349	-0.16420578153677212
1202	350-359	-0.19321009776869147
1202	360-369	-0.2904942777738313
1202	370-379	-0.1293302073200735
1202	380-389	-0.13007843472605174
1202	390-399	-0.23493146134845233
1202	400-409	-0.21002007345359175
1202	410-419	-0.2144547292059258
1202	420-429	-0.06462485217701186
1202	430-439	-0.13995825784379434
1202	440-449	-0.15617459648041887
1202	450-459	-0.17463425360946871
1202	460-468	-0.22424313802845575
1203	1	0.15722897845507333
1203	2	-0.832805272061016
1203	3	0.04910185877869111
1203	4	0.13142805787310152
1203	5	-0.08974187939371348
1203	6	0.04009570012000552
1203	7	0.08425719751957672
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2404	170-179	0.09508097862144638
2404	180-189	0.06392473367700546
2404	190-199	0.10537357003691028
2404	200-209	0.09332841771253442
2404	210-219	0.0594247071302334
2404	220-229	0.0774356633107871
2404	230-239	0.09697292988437312
2404	240-249	0.02188146525352863
2404	250-259	0.05561964503602468
2404	260-269	0.04815640489172779
2404	270-279	0.06052162714541254
2404	280-289	0.07362595957759765
2404	290-299	0.014975530136801751
2404	300-309	0.3138045518334991
2404	310-319	0.24402861593155478
2404	320-329	0.3364447333318381
2404	330-339	0.10120682041384299
2404	340-349	0.3025613911256002
2404	350-359	0.17571275521530083
2404	360-369	0.3326706213736017
2404	370-379	0.24722070416533626
2404	380-389	0.16930120067977583
2404	390-399	0.12876161413502274
2404	400-409	0.16582036461392136
2404	410-419	0.14600829151910233
2404	420-429	0.1457168682083747
2404	430-439	0.11212669301154676
2404	440-449	0.21618536285031809
2404	450-459	0.12536574639052844
2404	460-468	0.32077043085999435
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	4.0
23	23.0
24	44.0
25	78.0
26	87.0
27	132.0
28	173.0
29	254.0
30	353.0
31	442.0
32	596.0
33	829.0
34	1101.0
35	1453.0
36	2109.0
37	3298.0
38	7246.0
39	127461.0
40	2338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0033778990818870295	0.004053478898264436	0.1026881320893657	99.88988048993048
2	0.009458117429283684	0.006755798163774059	0.2675296072854527	99.71625647712149
3	0.0033778990818870295	0.204700684362354	99.64599617621825	0.1459252403375197
4	0.052019645861060254	99.53857898541423	0.0054046385310192475	0.4039967301936887
5	0.15268103850129372	0.010809277062038495	0.10066139264023348	99.73584829179643
6	99.90812114497267	0.07634051925064686	0.0114848568784159	0.004053478898264436
7	99.91285020368733	0.061477763290343934	0.023645293573209207	0.002026739449132218
8	99.87569331378656	0.06823356145411799	0.03715688990075733	0.018916234858567367
9	28.266935097047043	22.03538687078185	26.725937535890175	22.971740496280933
10-19	49.89825767965356	15.253173536187434	14.77276872876146	20.075800055397544
20-29	39.88197620607887	29.844346410306642	29.93804933083819	0.33562805277629526
30-39	30.22652191243134	29.99108234642382	10.05080360219158	29.731592138953257
40-49	29.871504718925017	32.8639179575871	10.169502975929092	27.09507434755879
50-59	30.122415062727587	22.914181095925578	16.909425014018282	30.053978827328553
60-69	33.02004445315192	10.06458543044568	24.291350551610922	32.62401956479148
70-79	40.107754980712194	36.972321494923015	3.1250971145986046	19.79482640976618
80-89	39.79881233068281	0.21145648252612806	47.00211456482526	12.987616621965802
90-99	12.971740496280933	36.84477202559096	36.983806351801434	13.19968112632667
100-109	48.59141608285311	25.509961424392486	3.355875179873126	22.542747312881282
110-119	44.08685254119348	40.166733098681945	12.789874409712136	2.9565399504124414
120-129	18.855635349038312	40.964052397970555	29.80246046169125	10.377851791299882
130-139	13.502678673971936	18.360975807486778	35.58508589997365	32.551259618567634
140-149	20.12282041061741	22.921680031887366	27.021165915647106	29.934333641848117
150-159	32.70968308550814	41.22259679369819	3.229203964302362	22.83851615649131
160-169	29.98007039541687	20.04573675356875	12.956067044540978	37.01812580647341
170-179	31.287992919923525	13.052607400301309	17.300991075590623	38.35840860418454
180-189	34.61650711723336	19.87677424149276	11.296302551664967	34.210416089608906
190-199	27.068051154903692	36.89989933860736	20.378527371116263	15.653522135372683
200-209	47.18661541267793	22.74042196715331	12.956472392430804	17.116490227737955
210-219	38.51703474506996	24.29074252977618	26.8790239222813	10.313198802872565
220-229	30.260368461231852	14.330263949034258	38.19262131724553	17.21674627248836
230-239	32.94559555738712	39.74469838739098	10.36298903533958	16.946717019882314
240-249	43.85830388931301	18.651880476418885	13.154349720647746	24.335465913620364
250-259	20.131535390248683	20.240371298667082	38.28112227319097	21.346971037893272
260-269	30.31704960782592	43.53699812864391	20.224832962890403	5.9211193006397735
270-279	26.766674998817734	9.055269184777835	25.5908958863945	38.58715993000993
280-289	30.106876726950905	32.61503435323366	13.018017713702784	24.260071206112645
290-299	28.574256355517125	22.951000195918148	24.379513717648173	24.09522973091656
300-309	31.57666817546159	37.08271123691909	17.1845886732288	14.156031914390526
310-319	26.98830164409326	13.182944559143039	39.616864656859036	20.211889139904663
320-329	54.47864548217068	23.029964829124676	12.778255940743222	9.713133747961427
330-339	29.90825564112958	30.091068774489933	29.710985002026753	10.289690582353735
340-349	40.04073773814349	25.571544385893795	20.199702742872585	14.188015133090124
350-359	34.45694882826012	29.98665038049118	19.785608893981323	15.770791897267381
360-369	35.68325687918443	27.359190374208715	21.570744296340337	15.386808450266521
370-379	34.074837740315594	31.40652984179723	18.254020263628494	16.26461215425868
380-389	41.36203152847007	25.734806301370234	12.985665315034733	19.917496855124963
390-399	39.79498009997642	5.705303993043944	3.7223955155866064	50.77732039139303
400-409	42.766649731377484	36.67359392059151	10.179955463792712	10.37980088423829
410-419	23.37319442755415	28.5670950045266	14.254192171010851	33.805518396908404
420-429	41.21371279395947	32.89528800056211	15.626604582281628	10.264394623196788
430-439	50.10553914887173	11.494977932108782	14.815843988663161	23.583638930356333
440-449	20.351482981336627	49.48654211810713	19.87797279396539	10.28400210659085
450-459	16.679790387737093	33.40621826916958	16.51926622410123	33.3947251189921
460-469	33.095031957424055	16.798814646940492	33.329778971760334	16.776374423875122
470-479	27.586206896551722	13.793103448275861	34.48275862068966	24.137931034482758
480-489	10.0	30.0	30.0	30.0
490-497	25.0	25.0	25.0	25.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.2
48	1.4
49	21.199999999999957
50	302.99999999999136
51	70.20000000000046
52	90.95000000000074
53	1301.550000000159
54	8635.099999996353
55	20192.600000038397
56	3196.9999999992833
57	144.00000000000037
58	11.79999999999999
59	1.2
60	0.2
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	1.351159632754812E-4
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	6.755889446625095E-5
380-389	0.0
390-399	6.75607201972773E-5
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-489	0.0
490-497	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
320-324	1.0
325-329	0.0
330-334	0.0
335-339	0.0
340-344	0.0
345-349	0.0
350-354	1.0
355-359	0.0
360-364	0.0
365-369	0.0
370-374	0.0
375-379	0.0
380-384	0.0
385-389	4.0
390-394	0.0
395-399	0.0
400-404	1.0
405-409	0.0
410-414	0.0
415-419	0.0
420-424	0.0
425-429	1.0
430-434	2.0
435-439	39578.0
440-444	3147.0
445-449	2.0
450-454	4.0
455-459	2.0
460-464	3614.0
465-469	101659.0
470-474	2.0
475-479	2.0
480-484	0.0
485-489	0.0
490-494	0.0
495-498	1.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	2.0780835151769006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	50.97529258777633	1.0593091520797726
2	12.028608582574773	0.4999290641192804
3	5.884265279583875	0.36683984029293143
4	4.031209362808843	0.33508758892319335
5	2.860858257477243	0.2972551192060586
6	2.503250975292588	0.3121178751663615
7	2.4707412223667102	0.35940846231277995
8	2.1781534460338103	0.3621107815782896
9	1.7555266579973992	0.32833179075941926
>10	13.97919375812744	5.684328574999493
>50	0.26007802340702213	0.36683984029293143
>100	0.5526657997399219	2.040926625276143
>500	0.0	0.0
>1k	0.22756827048114434	20.57140540869201
>5k	0.06501950585175553	9.139919335769925
>10k+	0.22756827048114434	58.27619054053142
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	14106	9.529728889819687	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	13012	8.790644570702806	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	12704	8.582565987258564	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	12408	8.382594361610852	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	12371	8.35759790840489	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	11085	7.488802264543544	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	10575	7.144256558191067	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8254	5.576235804379109	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	5275	3.5636835313908164	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4967	3.355604947946575	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4800	3.2427831186115483	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4761	3.216435505772829	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4461	3.013761560859608	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4330	2.925260604914168	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3950	2.6685402746907534	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3181	2.1490193958965285	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	261	0.17632633207450293	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	238	0.1607879962978226	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	220	0.1486275596030293	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	220	0.1486275596030293	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	219	0.1479519797866519	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	209	0.14119618162287784	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	208	0.14052060180650044	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	181	0.12227994676431046	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	179	0.12092878713155567	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	164	0.11079508988589458	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	158	0.10674161098763013	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0
320-329	0.0	0.0	0.0	0.0	0.0
330-339	0.0	0.0	0.0	0.0	0.0
340-349	0.0	0.0	0.0	0.0	0.0
350-359	0.0	0.0	0.0	0.0	0.0
360-369	0.0	0.0	0.0	0.0	0.0
370-379	0.0	0.0	0.0	0.0	0.0
380-389	0.0	0.0	0.0	0.0	0.0
390-399	0.0	0.0	0.0	0.0	0.0
400-409	0.0	0.0	0.0	0.0	0.0
410-419	0.0	0.0	0.0	0.0	0.0
420-429	0.0	0.0	0.0	0.0	0.0
430-439	0.0	0.0	0.0	0.0	0.0
440-449	0.0	0.0	0.0	0.0	0.0
450-459	0.0	0.0	0.0	0.0	0.0
460-469	0.0	0.0	0.0	0.0	0.0
470-479	0.0	0.0	0.0	0.0	0.0
480-486	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
