##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IAFS1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	160629
Sequences flagged as poor quality	0
Sequence length	350-475
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.791444882306436	40.0	40.0	40.0	40.0	40.0
2	39.33004625565745	40.0	40.0	40.0	40.0	40.0
3	39.38862845438869	40.0	40.0	40.0	40.0	40.0
4	39.2632712648401	40.0	40.0	40.0	40.0	40.0
5	39.31646215814081	40.0	40.0	40.0	40.0	40.0
6	39.36492165175653	40.0	40.0	40.0	40.0	40.0
7	39.422171587944895	40.0	40.0	40.0	40.0	40.0
8	39.5256149263209	40.0	40.0	40.0	40.0	40.0
9	39.07356703957567	40.0	40.0	40.0	40.0	40.0
10-19	39.57105877519003	40.0	40.0	40.0	40.0	40.0
20-29	39.58186877836506	40.0	40.0	40.0	40.0	40.0
30-39	39.61432368999371	40.0	40.0	40.0	40.0	40.0
40-49	39.558147034470736	40.0	40.0	40.0	40.0	40.0
50-59	39.57000790641789	40.0	40.0	40.0	40.0	40.0
60-69	39.42702251772719	40.0	40.0	40.0	40.0	40.0
70-79	39.50632824707868	40.0	40.0	40.0	40.0	40.0
80-89	39.60535146206476	40.0	40.0	40.0	40.0	40.0
90-99	39.4927528652983	40.0	40.0	40.0	40.0	40.0
100-109	39.39516401147988	40.0	40.0	40.0	40.0	40.0
110-119	39.40503645045415	40.0	40.0	40.0	40.0	40.0
120-129	39.34922834606453	40.0	40.0	40.0	40.0	40.0
130-139	39.291255003766445	40.0	40.0	40.0	40.0	40.0
140-149	39.404052817361745	40.0	40.0	40.0	40.0	40.0
150-159	39.484845202298466	40.0	40.0	40.0	40.0	40.0
160-169	39.600728386530456	40.0	40.0	40.0	40.0	40.0
170-179	39.690267635358495	40.0	40.0	40.0	40.0	40.0
180-189	39.745278872432756	40.0	40.0	40.0	40.0	40.0
190-199	39.68326889914026	40.0	40.0	40.0	40.0	40.0
200-209	39.75179201763069	40.0	40.0	40.0	40.0	40.0
210-219	39.69710201769294	40.0	40.0	40.0	40.0	40.0
220-229	39.70885456548942	40.0	40.0	40.0	40.0	40.0
230-239	39.69542486101513	40.0	40.0	40.0	40.0	40.0
240-249	39.764021440711204	40.0	40.0	40.0	40.0	40.0
250-259	39.741744018826	40.0	40.0	40.0	40.0	40.0
260-269	39.739890057212584	40.0	40.0	40.0	40.0	40.0
270-279	39.70400612591749	40.0	40.0	40.0	40.0	40.0
280-289	39.731661157076246	40.0	40.0	40.0	40.0	40.0
290-299	39.727832458646944	40.0	40.0	40.0	40.0	40.0
300-309	38.8324212937888	40.0	40.0	40.0	38.4	40.0
310-319	38.83157586737139	40.0	40.0	40.0	38.4	40.0
320-329	38.80521201028457	40.0	40.0	40.0	38.4	40.0
330-339	39.02281904263863	40.0	40.0	40.0	38.4	40.0
340-349	38.952239010390414	40.0	40.0	40.0	38.4	40.0
350-359	39.2092073376205	40.0	40.0	40.0	40.0	40.0
360-369	38.37210209184467	40.0	38.4	40.0	36.8	40.0
370-379	38.845450244048216	40.0	40.0	40.0	38.4	40.0
380-389	39.115685661006935	40.0	40.0	40.0	40.0	40.0
390-399	39.16250824918131	40.0	40.0	40.0	40.0	40.0
400-409	39.252496248933824	40.0	40.0	40.0	40.0	40.0
410-419	39.278863909451445	40.0	40.0	40.0	40.0	40.0
420-429	39.3306412841993	40.0	40.0	40.0	40.0	40.0
430-439	39.211657075058966	40.0	40.0	40.0	40.0	40.0
440-449	39.19132129875713	40.0	40.0	40.0	40.0	40.0
450-459	39.2624216381332	40.0	40.0	40.0	40.0	40.0
460-469	38.779341854465784	40.0	40.0	40.0	37.714285714285715	40.0
470-475	36.22222222222222	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.049015275449825424
1101	2	-0.01853689840385897
1101	3	0.16246089384213036
1101	4	-0.020174218886474193
1101	5	-0.26988507586764854
1101	6	0.15496785880147712
1101	7	-0.21611431363283629
1101	8	0.07090790878651632
1101	9	0.04300480265412432
1101	10-19	0.07926293464620215
1101	20-29	0.04879643470145112
1101	30-39	-0.005853231895301292
1101	40-49	0.05320781865798807
1101	50-59	0.0036063436235025392
1101	60-69	0.050383444082427786
1101	70-79	-0.0037231258919305787
1101	80-89	0.0387688486089246
1101	90-99	0.02081714464604545
1101	100-109	-0.09247179808597394
1101	110-119	0.005739019773258747
1101	120-129	-0.02517977730088461
1101	130-139	0.0307852919137801
1101	140-149	-0.01976346883125757
1101	150-159	-0.0040850804325174295
1101	160-169	-0.06412640121727975
1101	170-179	-0.1047215875938079
1101	180-189	-0.11691159973969434
1101	190-199	-0.046840607187085936
1101	200-209	-0.07311390726481903
1101	210-219	-3.4531006878779635E-4
1101	220-229	-0.013978440374181389
1101	230-239	-0.06406333273888976
1101	240-249	-0.10793659865255734
1101	250-259	-0.09931291789630592
1101	260-269	-0.10465372049998223
1101	270-279	-0.09774621141555428
1101	280-289	-0.08607521348935165
1101	290-299	-0.09907681275967661
1101	300-309	-0.29683332884519587
1101	310-319	-0.2060728320956855
1101	320-329	-0.2147546665746347
1101	330-339	-0.1492740534839001
1101	340-349	-0.051166271479658576
1101	350-359	-0.12278909372381008
1101	360-369	-0.1451231866836551
1101	370-379	-0.14312763256280192
1101	380-389	-0.14684156299716733
1101	390-399	-6.224217157608791E-4
1101	400-409	-0.13624175383428394
1101	410-419	0.02183188473478026
1101	420-429	-0.014364674036727365
1101	430-439	-0.04572704708590436
1101	440-449	0.06389469478245502
1101	450-459	0.1571904556500101
1101	460-468	0.19093330648431817
1102	1	0.060776100428668656
1102	2	0.022984550759261424
1102	3	0.22018933352487124
1102	4	0.29246293097799025
1102	5	-0.10460245750642372
1102	6	0.06204299899911092
1102	7	0.2130984282170587
1102	8	0.057182165488036674
1102	9	0.1895991832745736
1102	10-19	0.03335842680804291
1102	20-29	0.013376272057847416
1102	30-39	0.008659912977989848
1102	40-49	0.039047498534067415
1102	50-59	-0.050836236299907966
1102	60-69	0.099936676157931
1102	70-79	0.015180248891127235
1102	80-89	0.0252295574137662
1102	90-99	0.04444492887792961
1102	100-109	-0.02298970465913186
1102	110-119	-0.05589630859758188
1102	120-129	0.06254020032486096
1102	130-139	0.07640008186831437
1102	140-149	0.057106430628024896
1102	150-159	-0.017931300474693046
1102	160-169	-0.03242165529412233
1102	170-179	-0.012828668471001947
1102	180-189	-0.01800521303802327
1102	190-199	0.01374270104692954
1102	200-209	-0.0013807632520794755
1102	210-219	0.07983052860711837
1102	220-229	0.04559730937840811
1102	230-239	0.008048452468287337
1102	240-249	-0.009343594909175579
1102	250-259	-0.008180866779746054
1102	260-269	-0.031225296593966334
1102	270-279	-0.07783886376843441
1102	280-289	-0.07297982172480033
1102	290-299	-0.04903378535122016
1102	300-309	-0.3759162713462345
1102	310-319	-0.46335091823656427
1102	320-329	-0.2450961607731017
1102	330-339	-0.309721825381267
1102	340-349	-0.27648503286398807
1102	350-359	-0.2074871130287832
1102	360-369	-0.40608011624779294
1102	370-379	-0.3084776605306203
1102	380-389	-0.2727569711121376
1102	390-399	-0.2674267760894935
1102	400-409	-0.2381091433614202
1102	410-419	-0.23238264992379243
1102	420-429	-0.1877536561516706
1102	430-439	-0.19871815711859853
1102	440-449	-0.44412719831351666
1102	450-459	-0.15212913654856663
1102	460-468	-1.307863894770314
1103	1	0.14756373037361925
1103	2	0.13408559836967981
1103	3	0.023991324085308463
1103	4	-0.007268373553600327
1103	5	0.20354193360147121
1103	6	-0.08460233304609233
1103	7	-0.2253079158137794
1103	8	-0.07263699340457919
1103	9	0.16665440170285706
1103	10-19	-0.04174749153671087
1103	20-29	-0.05461273859939553
1103	30-39	-0.011797171223321357
1103	40-49	-0.12249583993165913
1103	50-59	-0.03274608746072971
1103	60-69	-0.11549872119940119
1103	70-79	-0.030834101660850877
1103	80-89	-0.08292892259508022
1103	90-99	-0.12090643824863179
1103	100-109	-0.08169614228052069
1103	110-119	-0.03700107970326627
1103	120-129	-0.03784925715182652
1103	130-139	-0.07283559525433247
1103	140-149	-0.050502582243190375
1103	150-159	-0.03743436481064322
1103	160-169	-0.07866142826154743
1103	170-179	-0.07913103641271135
1103	180-189	-0.1431126409078871
1103	190-199	-0.06110156627991614
1103	200-209	-0.05649654382751379
1103	210-219	-0.06527911323289715
1103	220-229	-0.04199137522142138
1103	230-239	-0.12453011831333782
1103	240-249	-0.04347697654731775
1103	250-259	-0.08168266311461991
1103	260-269	-0.0806350722785325
1103	270-279	-0.09643822574249583
1103	280-289	-0.08134862889806982
1103	290-299	-0.05012668152607347
1103	300-309	-0.598382030285137
1103	310-319	-0.42697740134497764
1103	320-329	-0.3531821835770046
1103	330-339	-0.38964614785609086
1103	340-349	-0.4347758479069128
1103	350-359	-0.3954330519030762
1103	360-369	-0.5449564257608159
1103	370-379	-0.3704285707742514
1103	380-389	-0.27317963814109447
1103	390-399	-0.2975206685509235
1103	400-409	-0.337049456738832
1103	410-419	-0.30874046775430486
1103	420-429	-0.19953600275876937
1103	430-439	-0.20968661247005826
1103	440-449	-0.3297936374536121
1103	450-459	-0.3116514561146957
1103	460-468	-0.05732516443516289
1104	1	0.006865276177549617
1104	2	-0.23745200166844427
1104	3	-0.030052241646252753
1104	4	0.1230135203540712
1104	5	-0.09394958166764411
1104	6	-0.035624075062614224
1104	7	0.13409745518256955
1104	8	0.0658283681283578
1104	9	-0.13192642706046342
1104	10-19	0.07037009985499054
1104	20-29	0.0064310279743367005
1104	30-39	0.01332239795319623
1104	40-49	0.06167711581839086
1104	50-59	0.10596709289213635
1104	60-69	0.011997253446828893
1104	70-79	0.04405075449870566
1104	80-89	0.04092874498046939
1104	90-99	0.08958160823781469
1104	100-109	-0.016280143669391123
1104	110-119	0.1623664376491618
1104	120-129	0.07113791958588678
1104	130-139	0.04649257064811252
1104	140-149	0.019813284629080385
1104	150-159	0.033232805663516274
1104	160-169	0.03447126714255688
1104	170-179	-0.07062826376945708
1104	180-189	-0.0026837918016369144
1104	190-199	0.07131402043455637
1104	200-209	0.05326404549108332
1104	210-219	0.026116331394241854
1104	220-229	0.04433876165764161
1104	230-239	0.0650043022910225
1104	240-249	-0.007949697754540352
1104	250-259	0.05303545999822745
1104	260-269	0.0204579857308147
1104	270-279	0.05512613055832816
1104	280-289	0.024138447418557973
1104	290-299	-0.021044734514376273
1104	300-309	-0.4168254693840012
1104	310-319	-0.25250478226088546
1104	320-329	-0.21771651907874912
1104	330-339	-0.22025517622248714
1104	340-349	-0.20883725114693164
1104	350-359	-0.08562548594004227
1104	360-369	-0.29199468449319
1104	370-379	-0.24301701792383312
1104	380-389	-0.139989277203874
1104	390-399	-0.11694244733178039
1104	400-409	-0.17187276668596496
1104	410-419	-0.09821701857928389
1104	420-429	-0.11627568747058348
1104	430-439	-0.14491485730914633
1104	440-449	0.06238243107429753
1104	450-459	0.1116899857252065
1104	460-468	0.025418459330126097
1201	1	0.11489253697533997
1201	2	0.0014186224232162203
1201	3	0.03208883157128639
1201	4	0.133158713733593
1201	5	0.003241088705287609
1201	6	0.3309010198281044
1201	7	-0.12385008330284819
1201	8	0.03736820811933228
1201	9	0.07780675012060811
1201	10-19	0.1785356400618454
1201	20-29	0.14984229633516577
1201	30-39	0.12847387034669566
1201	40-49	0.1694066525823672
1201	50-59	0.12167450495066845
1201	60-69	0.1334813583698633
1201	70-79	0.23952986443083546
1201	80-89	0.12731245623049148
1201	90-99	0.13444339937947092
1201	100-109	0.1385434089826063
1201	110-119	0.08752059096998721
1201	120-129	0.08035607651891041
1201	130-139	0.14202529912661532
1201	140-149	0.11646000895920139
1201	150-159	0.049386098265564726
1201	160-169	0.09108186950460606
1201	170-179	0.08308270404530305
1201	180-189	0.1090516147821532
1201	190-199	0.1227723037944557
1201	200-209	0.10288236610178103
1201	210-219	0.10767296245631286
1201	220-229	0.13929438525543247
1201	230-239	0.12167628377850548
1201	240-249	0.0925773161157224
1201	250-259	0.04805740127181224
1201	260-269	0.13170800336850874
1201	270-279	0.09929652465464045
1201	280-289	0.10767967020362335
1201	290-299	0.062214285395050695
1201	300-309	0.09934687191215374
1201	310-319	0.00947684633190704
1201	320-329	0.038470073462633536
1201	330-339	0.21452350617229143
1201	340-349	0.19387189645927805
1201	350-359	0.14665852303402005
1201	360-369	0.1754574468440211
1201	370-379	0.11818149768904362
1201	380-389	0.11638498024979782
1201	390-399	0.09805522449448745
1201	400-409	0.11624367022052695
1201	410-419	0.10420851513690366
1201	420-429	0.09915939881295088
1201	430-439	0.1809243338977211
1201	440-449	0.1980833028638429
1201	450-459	0.06187795565001153
1201	460-468	0.28353260783057976
1202	1	-0.008063796562723269
1202	2	-0.00867175223678629
1202	3	0.08338888129197386
1202	4	0.06182415200682101
1202	5	-0.11427521675521035
1202	6	0.07674056824727415
1202	7	0.06511777373555816
1202	8	0.11232945523114068
1202	9	0.02089118754566499
1202	10-19	0.0994095542497817
1202	20-29	0.063699744201557
1202	30-39	0.03703752744944211
1202	40-49	-0.07308113660192106
1202	50-59	-0.01902918900388073
1202	60-69	-0.07135909672066276
1202	70-79	-0.01822217557969452
1202	80-89	-0.007117656957184693
1202	90-99	-0.012712284864917933
1202	100-109	-0.036493881532443595
1202	110-119	0.007265553094526922
1202	120-129	-0.04148592868713763
1202	130-139	-0.013556579145429737
1202	140-149	0.047829375166685395
1202	150-159	0.024873799707457067
1202	160-169	-0.018603427795852667
1202	170-179	-0.08602758813685796
1202	180-189	0.013412411483038511
1202	190-199	-0.029709343422645418
1202	200-209	0.008053317521735437
1202	210-219	0.055548134285153594
1202	220-229	0.04018935761659037
1202	230-239	-0.011981696391714536
1202	240-249	0.014593343725330499
1202	250-259	0.053310245587567806
1202	260-269	-0.0014030492476848622
1202	270-279	-0.05015216067189243
1202	280-289	-0.017901623119179533
1202	290-299	0.001980598167413916
1202	300-309	-0.2255237080650332
1202	310-319	-0.22258714711666272
1202	320-329	-0.20583983265301242
1202	330-339	-0.2719729402609872
1202	340-349	-0.20863753584747258
1202	350-359	-0.180755123832121
1202	360-369	-0.22244843482073406
1202	370-379	-0.16245668194887486
1202	380-389	-0.14255158200342066
1202	390-399	-0.1286038378318466
1202	400-409	-0.11312955665204072
1202	410-419	-0.09901163051546291
1202	420-429	-0.20114477479999238
1202	430-439	-0.10462389144784368
1202	440-449	-0.0329604434637929
1202	450-459	-0.05327648450442979
1202	460-468	-0.635050783940386
1203	1	0.0720418088978505
1203	2	-0.3397835072836273
1203	3	0.08815400573255516
1203	4	-0.03003328062140298
1203	5	0.1707951332238622
1203	6	0.17938392444033013
1203	7	0.09335435572467077
1203	8	0.15315498084368784
1203	9	0.15125597713966243
1203	10-19	0.06321714625210717
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2404	180-189	-0.07371917547298068
2404	190-199	-0.042043230789886366
2404	200-209	-0.0424589687655228
2404	210-219	-0.06412163028945628
2404	220-229	0.02668419573724634
2404	230-239	0.04490197989642297
2404	240-249	0.0439501181554931
2404	250-259	0.033887845450230714
2404	260-269	-0.015920298430295077
2404	270-279	0.01171704050483413
2404	280-289	-3.2782346096382753E-4
2404	290-299	0.02943883103331757
2404	300-309	0.30910185084594133
2404	310-319	0.23642760891333836
2404	320-329	0.36262237208983095
2404	330-339	0.08826303136623892
2404	340-349	0.22602313788898698
2404	350-359	0.03339224918876482
2404	360-369	0.06636710309937399
2404	370-379	0.10731257463125843
2404	380-389	0.06461184239436335
2404	390-399	0.09191570551935513
2404	400-409	0.15862329740294
2404	410-419	0.09049137765458681
2404	420-429	0.011338935822635676
2404	430-439	0.13892286676872345
2404	440-449	0.19343541396423092
2404	450-459	0.1891084389213873
2404	460-468	0.3379790862588834
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	9.0
23	19.0
24	37.0
25	69.0
26	115.0
27	122.0
28	203.0
29	259.0
30	343.0
31	514.0
32	635.0
33	854.0
34	1103.0
35	1443.0
36	2069.0
37	3271.0
38	7186.0
39	139177.0
40	3200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.00996084144208082	0.0037353155407803076	0.09027012556885743	99.89603371744829
2	0.00747077060998842	0.009338463262485525	0.2508933796521111	99.73229738647541
3	0.0031127629506502563	0.19112364516992575	99.67627265313237	0.12949093874705064
4	0.059142496062354874	99.59409571123521	0.011828499212470974	0.33493329348996753
5	0.14505475350030195	0.013073604392731076	0.0790641789465165	99.76280746316046
6	99.92404858400413	0.05851994347222481	0.012451051802601025	0.00498042072104041
7	99.93089666249556	0.0498042072104041	0.01618636734338133	0.0031127629506502563
8	99.89727882262855	0.053539522751184405	0.031127629506502563	0.018054025113771483
9	28.458124000024903	21.788095549371533	26.431092766561452	23.32268768404211
10-19	49.81292294666592	15.291261229292344	14.854789608352165	20.04102621568957
20-29	39.78752280098861	29.76504865248492	29.957043871281027	0.4903846752454414
30-39	30.03691736859471	30.09655790672917	10.068231763878254	29.798292960797863
40-49	29.931643725603717	34.81637811354114	10.231278287233314	25.020699873621826
50-59	30.20874188347061	24.866057810233517	15.025431273306813	29.89976903298906
60-69	34.94369011822273	10.031750182096633	20.51373039737532	34.51082930230531
70-79	40.2082438413985	35.546445535986656	4.763212122344035	19.482098500270812
80-89	39.68087954229934	0.23364398707580825	45.068947699356904	15.016528771267954
90-99	14.929433663908759	34.93802488965255	35.03744653829632	15.095094908142364
100-109	41.05167809050669	29.46759302492078	5.334341868529345	24.14638701604318
110-119	40.3556642947413	40.294716396167566	14.547373139345945	4.802246169745189
120-129	24.492214979860425	35.33894875769631	29.879971860622927	10.288864401820344
130-139	18.044126527588418	21.237821314955582	26.3997161160189	34.31833604143711
140-149	20.194609939674653	24.836362051684315	25.256896326317165	29.712131682323868
150-159	34.28895155918296	35.73588828916323	5.29717547889858	24.677984672755233
160-169	30.050924801872636	20.052356672829937	14.827646315422497	35.06907220987493
170-179	38.66711490453156	14.9468651364324	15.457171494562003	30.928848464474036
180-189	30.81604193514247	20.311774337137127	18.31282022548855	30.55936350223185
190-199	25.12454164565552	34.962117674890585	20.686052954323316	19.22728772513058
200-209	45.37219306600925	24.55776976760112	14.707244644491343	15.362792521898289
210-219	43.97250807761986	20.410262156895705	25.131638745183	10.48559102030144
220-229	30.3236028363496	10.821707163712654	43.06246070136775	15.792229298569996
230-239	34.801810382932096	39.67459176113902	10.553636018402655	14.969961837526224
240-249	40.21341102789658	24.153795391865728	15.180260102472158	20.452533477765535
250-259	19.91857012121099	20.466416400525432	30.81990176120128	28.795111717062298
260-269	30.205006567929825	40.14524151927734	20.507006829401913	9.14274508339092
270-279	24.95925393297599	14.741920823761587	28.8707518567631	31.42807338649932
280-289	29.616507604479885	34.26666417645631	15.48898393191765	20.627844287146157
290-299	33.96927080415118	24.985525652279474	20.71014573956135	20.335057804007995
300-309	38.907731480616825	35.25204041611415	15.508407572729707	10.331820530539318
310-319	25.260320365562883	15.113397954292187	39.48272105286094	20.14356062728399
320-329	50.67758623909755	25.146642262605134	14.502611608115595	9.673159890181724
330-339	29.958662508015365	30.06804499810121	29.625721382813815	10.347571111069607
340-349	39.88625964178324	29.27080415118067	20.27828100778813	10.564655199247957
350-359	30.976606006348884	29.75819088607311	19.87384413674688	19.391358970831128
360-369	39.23851016593971	25.574974194488263	16.233316689971524	18.9531989496005
370-379	30.383193047116247	26.10848939137364	23.400986153999405	20.10733140751071
380-389	36.031646617749054	29.23383707769334	14.770787392652895	19.963728911904713
390-399	39.78695321935974	9.454682422084149	5.723001830384381	45.03536252817174
400-409	44.444935593726846	34.66439631181477	10.512261783951041	10.378406310507343
410-419	25.29582059631057	34.07885643844828	10.56941495819351	30.055908007047645
420-429	35.60782365400204	34.756313947277	19.259111222346323	10.376751176374636
430-439	43.781858839205526	12.262587571882017	17.89274053170412	26.062813057208334
440-449	20.469695102991476	49.2390188382585	19.95071897495468	10.340567083795344
450-459	16.72999808825647	33.36983740739255	16.53280528302065	33.36735922133034
460-469	33.02304052664061	16.789438703653886	33.4295333343883	16.757987435317204
470-475	30.76923076923077	15.384615384615385	30.76923076923077	23.076923076923077
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	1.0
49	52.80000000000021
50	732.2000000000295
51	152.59999999999988
52	136.5000000000008
53	2183.600000000205
54	14359.050000017176
55	16587.550000025283
56	2409.5999999999995
57	233.79999999999526
58	17.59999999999997
59	0.0
60	0.2
61	0.2
62	0.0
63	0.0
64	0.2
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018676577703901538
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	0.0
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-475	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
350-354	3.0
355-359	0.0
360-364	2.0
365-369	0.0
370-374	0.0
375-379	0.0
380-384	0.0
385-389	2.0
390-394	0.0
395-399	1.0
400-404	0.0
405-409	0.0
410-414	0.0
415-419	0.0
420-424	0.0
425-429	1.0
430-434	10.0
435-439	72774.0
440-444	3087.0
445-449	9.0
450-454	1.0
455-459	0.0
460-464	3112.0
465-469	81624.0
470-474	1.0
475-476	2.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.8415105616046916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	49.32386747802569	0.9083042289997447
2	11.257606490872211	0.4146200250266141
3	6.085192697768763	0.3361783986702277
4	4.935767410412441	0.3635707126359499
5	3.1440162271805274	0.28948695441047384
6	2.8735632183908044	0.3175018209663262
7	2.366463826910074	0.3050507691637251
8	1.8931710615280595	0.2789035603782629
9	1.9945909398242054	0.3305754253590572
>10	14.705882352941178	4.73887031606995
>50	0.1352265043948614	0.15003517422134235
>100	0.7437457741717377	2.7952611296839303
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.4394861392832995	68.90163046523355
>10k+	0.10141987829614604	19.870011019180843
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	11078	6.896637593460708	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	10507	6.541160064496449	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	10332	6.432213361223689	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	9816	6.110976224716583	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9728	6.056191596785139	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9414	5.860710083484302	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	9264	5.767327194964795	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	9233	5.7480280646707635	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9163	5.704449383361659	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	8536	5.314108909350117	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8512	5.299167647186996	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	8464	5.269285122860754	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	8273	5.150377578145914	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	7514	4.677860162237205	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	6674	4.154915986527962	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	6085	3.788232510941362	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	382	0.23781508942967958	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	330	0.2054423547429169	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	326	0.2029521443823967	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	321	0.19983938143174643	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	320	0.1992168288416164	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	299	0.1861432244488853	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	268	0.16684409415485374	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	218	0.1357164646483512	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	207	0.12886838615692062	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	190	0.11828499212470975	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	188	0.11703988694444963	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	168	0.10458883514184861	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	166	0.1033437299615885	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	163	0.10147607219119835	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0
320-329	0.0	0.0	0.0	0.0	0.0
330-339	0.0	0.0	0.0	0.0	0.0
340-349	0.0	0.0	0.0	0.0	0.0
350-359	0.0	0.0	0.0	0.0	0.0
360-369	0.0	0.0	0.0	0.0	0.0
370-379	0.0	0.0	0.0	0.0	0.0
380-389	0.0	0.0	0.0	0.0	0.0
390-399	0.0	0.0	0.0	0.0	0.0
400-409	0.0	0.0	0.0	0.0	0.0
410-419	0.0	0.0	0.0	0.0	0.0
420-429	0.0	0.0	0.0	0.0	0.0
430-439	0.0	0.0	0.0	0.0	0.0
440-449	0.0	0.0	0.0	0.0	0.0
450-459	0.0	0.0	0.0	0.0	0.0
460-464	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
