##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	DVFS2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	95758
Sequences flagged as poor quality	0
Sequence length	300-499
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30785939555964	34.0	34.0	34.0	20.0	34.0
2	33.13371206583262	34.0	34.0	34.0	34.0	34.0
3	33.616689989348146	34.0	34.0	34.0	34.0	34.0
4	33.440642035130224	34.0	34.0	34.0	34.0	34.0
5	33.25781657929364	34.0	34.0	34.0	34.0	34.0
6	33.13788926251593	34.0	34.0	34.0	34.0	34.0
7	33.55999498736398	34.0	34.0	34.0	34.0	34.0
8	33.49623007999332	34.0	34.0	34.0	34.0	34.0
9	33.46568432924664	34.0	34.0	34.0	34.0	34.0
10-19	33.420127822218504	34.0	34.0	34.0	34.0	34.0
20-29	33.686576578458194	34.0	34.0	34.0	34.0	34.0
30-39	33.61642369305959	34.0	34.0	34.0	34.0	34.0
40-49	33.59013346143403	34.0	34.0	34.0	34.0	34.0
50-59	33.5299003738591	34.0	34.0	34.0	34.0	34.0
60-69	33.4864763257378	34.0	34.0	34.0	34.0	34.0
70-79	33.52355730069551	34.0	34.0	34.0	34.0	34.0
80-89	33.64614235886296	34.0	34.0	34.0	34.0	34.0
90-99	33.30880135341173	34.0	34.0	34.0	34.0	34.0
100-109	33.4411151026546	34.0	34.0	34.0	34.0	34.0
110-119	33.24577163265732	34.0	34.0	34.0	34.0	34.0
120-129	33.39919066814261	34.0	34.0	34.0	34.0	34.0
130-139	33.25434846174733	34.0	34.0	34.0	34.0	34.0
140-149	33.34124981724766	34.0	34.0	34.0	34.0	34.0
150-159	33.19607030222018	34.0	34.0	34.0	34.0	34.0
160-169	33.20242172977714	34.0	34.0	34.0	34.0	34.0
170-179	33.0448380291986	34.0	34.0	34.0	33.1	34.0
180-189	33.06776144029741	34.0	34.0	34.0	34.0	34.0
190-199	33.127024373942646	34.0	34.0	34.0	34.0	34.0
200-209	33.173363061049734	34.0	34.0	34.0	34.0	34.0
210-219	33.24712713298105	34.0	34.0	34.0	34.0	34.0
220-229	33.28938156603103	34.0	34.0	34.0	34.0	34.0
230-239	33.3444192652311	34.0	34.0	34.0	34.0	34.0
240-249	33.345718373399606	34.0	34.0	34.0	34.0	34.0
250-259	33.29210196537104	34.0	34.0	34.0	33.8	34.0
260-269	33.421050982685514	34.0	34.0	34.0	34.0	34.0
270-279	33.38376010359448	34.0	34.0	34.0	34.0	34.0
280-289	33.34529856513294	34.0	34.0	34.0	34.0	34.0
290-299	33.32517805300863	34.0	34.0	34.0	34.0	34.0
300-309	31.652607441791474	34.0	34.0	34.0	22.8	34.0
310-319	31.30795904471416	34.0	34.0	34.0	20.0	34.0
320-329	31.732944842839082	34.0	34.0	34.0	20.0	34.0
330-339	31.9333178673617	34.0	34.0	34.0	22.8	34.0
340-349	31.972455114660523	34.0	34.0	34.0	20.0	34.0
350-359	32.22035423773633	34.0	34.0	34.0	25.6	34.0
360-369	32.02711692049685	34.0	34.0	34.0	22.8	34.0
370-379	32.167787580700804	34.0	34.0	34.0	27.0	34.0
380-389	32.45234264700808	34.0	34.0	34.0	32.6	34.0
390-399	32.26437049691462	34.0	34.0	34.0	31.2	34.0
400-409	32.65035088636721	34.0	34.0	34.0	32.6	34.0
410-419	32.933300745831765	34.0	34.0	34.0	34.0	34.0
420-429	32.874093258178235	34.0	34.0	34.0	34.0	34.0
430-439	32.837172867520074	34.0	34.0	34.0	34.0	34.0
440-449	33.02938852115253	34.0	34.0	34.0	34.0	34.0
450-459	33.01701273668466	34.0	34.0	34.0	34.0	34.0
460-469	32.2889450035979	34.0	34.0	34.0	30.0	34.0
470-479	32.63040293040293	NaN	NaN	NaN	NaN	NaN
480-489	32.09444444444445	NaN	NaN	NaN	NaN	NaN
490-499	31.3	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.25082153249790196
1101	2	-0.014748987787804424
1101	3	-0.3735028875141708
1101	4	-0.14099569045579585
1101	5	0.16367906468121163
1101	6	-0.5404456607061405
1101	7	-0.28080805494799677
1101	8	-0.5334993602607483
1101	9	-0.1579036865790968
1101	10-19	-0.21620943484361987
1101	20-29	-0.149482235489117
1101	30-39	-0.1951791238331424
1101	40-49	-0.1806088757679447
1101	50-59	-0.3130084941880611
1101	60-69	-0.21965754408627447
1101	70-79	-0.357618854438428
1101	80-89	-0.2980830900516551
1101	90-99	-0.5106389870165984
1101	100-109	-0.43722291270471914
1101	110-119	-0.49703728862057517
1101	120-129	-0.3208397543605912
1101	130-139	-0.36332268566685855
1101	140-149	-0.3077850881107551
1101	150-159	-0.396312554329711
1101	160-169	-0.6826239003509116
1101	170-179	-0.8220517257096205
1101	180-189	-0.8906472152452949
1101	190-199	-0.9046342103631062
1101	200-209	-0.6955192865347257
1101	210-219	-0.9187472725103945
1101	220-229	-0.8118072816010624
1101	230-239	-0.8087325894789785
1101	240-249	-0.8317640856439823
1101	250-259	-0.6929106118286725
1101	260-269	-0.5991939852730965
1101	270-279	-0.6994394874989425
1101	280-289	-0.8144497156030894
1101	290-299	-0.866461690701982
1101	300-309	-1.7917986812463091
1101	310-319	-1.9165699530578593
1101	320-329	-1.9116656275809518
1101	330-339	-1.8418246562708198
1101	340-349	-1.677684541406336
1101	350-359	-1.5768241155269465
1101	360-369	-1.6239199382053613
1101	370-379	-1.5212647309781175
1101	380-389	-1.4348496165612978
1101	390-399	-1.5357182556231095
1101	400-409	-1.3469864275544339
1101	410-419	-1.079609078888776
1101	420-429	-1.0263662506049158
1101	430-439	-1.0498173726198452
1101	440-449	-0.6984125896900863
1101	450-459	-0.7822025232826846
1101	460-469	-0.36070336966418637
1101	470-479	-3.0290322580645164
1101	480-489	-1.096774193548387
1101	490	-0.6451612903225806
1102	1	-0.08055846001666822
1102	2	0.17653583282920948
1102	3	0.19376439580936022
1102	4	-0.015208383606626796
1102	5	-0.2225905177387233
1102	6	0.24605917808977296
1102	7	-0.1441454874454564
1102	8	0.255015851202657
1102	9	0.2183568063899486
1102	10-19	0.06599876704913754
1102	20-29	-0.025223025736664795
1102	30-39	0.08351199523867336
1102	40-49	-0.006799326497777258
1102	50-59	-0.013455481302422356
1102	60-69	-0.034038124741783804
1102	70-79	-0.0598569980517496
1102	80-89	0.013233810980267435
1102	90-99	-0.05275201701178389
1102	100-109	-0.120723955318212
1102	110-119	-0.08528195525159532
1102	120-129	-0.09947098999124648
1102	130-139	-0.15155345078475335
1102	140-149	-0.14642808656020634
1102	150-159	-0.34216188331436825
1102	160-169	-0.30226459970395325
1102	170-179	-0.5480828437120735
1102	180-189	-0.5655592614410878
1102	190-199	-0.6258532661295462
1102	200-209	-0.4334779027995026
1102	210-219	-0.41778592941035697
1102	220-229	-0.2641913911022158
1102	230-239	-0.3789491322795939
1102	240-249	-0.33506302164354906
1102	250-259	-0.3158042652532558
1102	260-269	-0.3415754214063398
1102	270-279	-0.43292395523138794
1102	280-289	-0.33938261406858317
1102	290-299	-0.3787463509169129
1102	300-309	-1.505896954250641
1102	310-319	-1.520817399991504
1102	320-329	-1.233452506691247
1102	330-339	-1.0198388785881
1102	340-349	-1.1209995176133596
1102	350-359	-0.8730514587339187
1102	360-369	-0.9006113780813116
1102	370-379	-0.6929569728644438
1102	380-389	-0.5767188354031951
1102	390-399	-0.6081675953012429
1102	400-409	-0.5863277097016777
1102	410-419	-0.37163067970148234
1102	420-429	-0.46776174865334497
1102	430-439	-0.41136044057685694
1102	440-449	-0.3611048649109989
1102	450-459	-0.25023257751963257
1102	460-469	0.14904015308189855
1102	470-479	-3.0290322580645164
1102	480-489	-1.096774193548387
1102	490	-0.6451612903225806
1103	1	-0.5727194769658226
1103	2	-0.17828408242503002
1103	3	-0.12265509571606259
1103	4	-0.15922321411510154
1103	5	-0.18662653468787482
1103	6	-0.38704463546955026
1103	7	-0.14287430100478105
1103	8	-0.4110328776109
1103	9	-0.05690802411852758
1103	10-19	-0.04337623295086246
1103	20-29	-0.031054593533283992
1103	30-39	-0.03174753865963709
1103	40-49	-0.056094877345231
1103	50-59	-0.05888980333632077
1103	60-69	-0.14444066711465808
1103	70-79	-0.17610170144158133
1103	80-89	-0.05460517207058757
1103	90-99	-0.20670858480839627
1103	100-109	-0.09624302311481614
1103	110-119	-0.21500653152278204
1103	120-129	-0.08078454931327883
1103	130-139	0.029288710232201254
1103	140-149	-0.13757215435681758
1103	150-159	-0.3294976883991083
1103	160-169	-0.20982815902598873
1103	170-179	-0.48112309794937147
1103	180-189	-0.45407091398345756
1103	190-199	-0.467691189858364
1103	200-209	-0.2571537502571246
1103	210-219	-0.27731982771544494
1103	220-229	-0.2256055860174584
1103	230-239	-0.2571641746524804
1103	240-249	-0.23022721655880218
1103	250-259	-0.1469218499990248
1103	260-269	-0.26558495530463944
1103	270-279	-0.0626591247229129
1103	280-289	-0.16327562254316064
1103	290-299	-0.11608215600165295
1103	300-309	-0.758804793233697
1103	310-319	-0.8501553237203154
1103	320-329	-0.734040430420059
1103	330-339	-0.6932816751982678
1103	340-349	-0.7465291786303112
1103	350-359	-0.37996883161526895
1103	360-369	-0.6225921276429709
1103	370-379	-0.32529744361519874
1103	380-389	-0.29693242660451347
1103	390-399	-0.40671496260372564
1103	400-409	-0.29124243261757243
1103	410-419	-0.33188996454288855
1103	420-429	-0.36329652363873777
1103	430-439	-0.1908345826841611
1103	440-449	-0.3695746676339411
1103	450-459	-0.11996004204888067
1103	460-469	0.4755819285178049
1103	470-479	-3.0290322580645164
1103	480-489	-1.096774193548387
1103	490	-0.6451612903225806
1104	1	-0.007014348760691291
1104	2	-0.2341334413993863
1104	3	0.11436413505317233
1104	4	-0.14295718847407102
1104	5	0.08300487556853398
1104	6	0.08194574914583796
1104	7	-0.032617890748369405
1104	8	-0.07938223658526056
1104	9	-0.01964840873391438
1104	10-19	-0.15086020730984728
1104	20-29	-0.021046580712770435
1104	30-39	-0.032348500198096986
1104	40-49	-0.05392340298624987
1104	50-59	-0.05156608538759855
1104	60-69	-0.0051778466018390645
1104	70-79	-0.08328920144158758
1104	80-89	-0.06107953104494612
1104	90-99	-0.1760114694237842
1104	100-109	-0.04680392055071536
1104	110-119	-0.24228217254842122
1104	120-129	-0.19155378008250779
1104	130-139	-0.2729148154088321
1104	140-149	-0.21616189794656293
1104	150-159	-0.37195762429654877
1104	160-169	-0.41774482569265814
1104	170-179	-0.7569724569237266
1104	180-189	-0.5311622601373074
1104	190-199	-0.6073145872942689
1104	200-209	-0.5096056733340504
1104	210-219	-0.5020634174590342
1104	220-229	-0.4839469321713068
1104	230-239	-0.47124270029350157
1104	240-249	-0.3469980498921359
1104	250-259	-0.39000678589646043
1104	260-269	-0.3924359168431053
1104	270-279	-0.5778033554921365
1104	280-289	-0.5504310712611016
1104	290-299	-0.48463183548883393
1104	300-309	-1.7980916522080577
1104	310-319	-1.6317017980792912
1104	320-329	-1.6284394688815986
1104	330-339	-1.3289707777623683
1104	340-349	-1.3278432811944079
1104	350-359	-1.0356579341793726
1104	360-369	-1.1773464932486277
1104	370-379	-0.9898029094137719
1104	380-389	-0.7386603825566134
1104	390-399	-1.04174430108133
1104	400-409	-0.7452423120484823
1104	410-419	-0.36103854584663253
1104	420-429	-0.5221935174495229
1104	430-439	-0.6518429198847571
1104	440-449	-0.45970609339572377
1104	450-459	-0.2797793245082403
1104	460-469	-0.5913148903283449
1104	470-479	-3.0290322580645164
1104	480-489	-1.096774193548387
1104	490	-0.6451612903225806
1201	1	-1.0615224085619133
1201	2	-0.2717388869852897
1201	3	-0.39816405337078464
1201	4	0.09931709533115196
1201	5	-0.2380821535803861
1201	6	0.08617401273892256
1201	7	0.20411266967926167
1201	8	0.05727941880603282
1201	9	-0.12118937591005619
1201	10-19	-0.1061836433743295
1201	20-29	-0.1144859860414229
1201	30-39	-0.14271557611891694
1201	40-49	-0.10747618842015072
1201	50-59	-0.1724871160399104
1201	60-69	-0.04755548796156717
1201	70-79	-0.10100092782595027
1201	80-89	-0.005606800735080242
1201	90-99	-0.10931139425465375
1201	100-109	-0.11793479835911569
1201	110-119	-0.266199528265453
1201	120-129	-0.1792291747204402
1201	130-139	-0.08249365947464327
1201	140-149	-0.13328062341219038
1201	150-159	-0.252346833350245
1201	160-169	-0.32176219811394446
1201	170-179	-0.492053472542203
1201	180-189	-0.3687624856447016
1201	190-199	-0.42343732178019167
1201	200-209	-0.19972093266754598
1201	210-219	-0.3082888830900288
1201	220-229	-0.28872956810216266
1201	230-239	-0.30907887334954864
1201	240-249	-0.30279338105391673
1201	250-259	-0.3928715649827339
1201	260-269	-0.36265641295936035
1201	270-279	-0.4238663722799103
1201	280-289	-0.4976821779177527
1201	290-299	-0.39525051919383714
1201	300-309	-1.4394430261327145
1201	310-319	-1.3176848432642885
1201	320-329	-1.017746660061757
1201	330-339	-1.303320355980027
1201	340-349	-1.0737726639723313
1201	350-359	-0.8882648413872616
1201	360-369	-1.0093862911271998
1201	370-379	-0.8602478689059652
1201	380-389	-0.6676767109621835
1201	390-399	-0.7166962763590412
1201	400-409	-0.6427899191644926
1201	410-419	-0.18290545472494557
1201	420-429	-0.5270053858200185
1201	430-439	-0.46044618098827783
1201	440-449	-0.4354920905754298
1201	450-459	-0.4258070030940644
1201	460-469	-0.23555896593619963
1201	470-479	-3.0290322580645164
1201	480-489	-1.096774193548387
1201	490	-0.6451612903225806
1202	1	0.16920995246360349
1202	2	0.13009617283022834
1202	3	-7.894801004439955E-4
1202	4	-0.022201817718702443
1202	5	-0.21426354669988967
1202	6	-0.28277474057465213
1202	7	-0.19550192863240312
1202	8	-0.2061436133466401
1202	9	-0.012660651746152496
1202	10-19	-0.17586309481272622
1202	20-29	0.0026193929532070115
1202	30-39	-0.07869292154252605
1202	40-49	-0.07449108605393917
1202	50-59	-0.04935714567866967
1202	60-69	-0.11574321966721612
1202	70-79	-0.11410751975989797
1202	80-89	-0.10728535225076996
1202	90-99	-0.07728197069428688
1202	100-109	0.006637982891177785
1202	110-119	-0.039882657648902864
1202	120-129	0.035198934170210805
1202	130-139	-0.01720622220023671
1202	140-149	0.024537455252790608
1202	150-159	-0.10933346167238511
1202	160-169	-0.09402110196893432
1202	170-179	-0.3142893892406633
1202	180-189	-0.2543402458152926
1202	190-199	-0.36044175292143166
1202	200-209	-0.2950873088156811
1202	210-219	-0.1929129208085385
1202	220-229	-0.2025453382697151
1202	230-239	-0.06996353651434362
1202	240-249	-0.08224579763988515
1202	250-259	-0.16792316381283
1202	260-269	-0.07609265049983804
1202	270-279	-0.12034493553371561
1202	280-289	-0.1066305399605767
1202	290-299	-0.06651852987553042
1202	300-309	-1.021399575515975
1202	310-319	-1.2284389198164085
1202	320-329	-0.8944073598494917
1202	330-339	-0.9116544229460182
1202	340-349	-0.6229464337535262
1202	350-359	-0.6634186050291682
1202	360-369	-0.6352992781274018
1202	370-379	-0.574150070309269
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2402	20-29	0.038640945051923836
2402	30-39	0.07492951117959734
2402	40-49	0.10560025931071948
2402	50-59	0.10295304232283797
2402	60-69	0.09693795024868734
2402	70-79	0.0871689979153274
2402	80-89	0.056174570694686565
2402	90-99	0.10956854541667838
2402	100-109	0.17362233058293697
2402	110-119	0.25215986719104677
2402	120-129	0.1221334571175916
2402	130-139	0.08660884688814718
2402	140-149	0.16508861734736513
2402	150-159	0.21924527783882297
2402	160-169	0.41033663197078596
2402	170-179	0.6278057605715333
2402	180-189	0.6334401390712046
2402	190-199	0.6138753294342436
2402	200-209	0.6046288060129683
2402	210-219	0.5014663459179971
2402	220-229	0.4937583609278704
2402	230-239	0.44720379158546564
2402	240-249	0.41973560498460216
2402	250-259	0.4423536725090216
2402	260-269	0.41106395948635566
2402	270-279	0.3615269685246787
2402	280-289	0.3599830189359423
2402	290-299	0.36362544046136946
2402	300-309	1.2100788482454128
2402	310-319	1.3338951184018697
2402	320-329	1.1379159602551745
2402	330-339	1.088826443772792
2402	340-349	0.9498414001478288
2402	350-359	0.9544061683847289
2402	360-369	0.9524694437618351
2402	370-379	0.8493439699662275
2402	380-389	0.7687299628964368
2402	390-399	0.7595995854715554
2402	400-409	0.5509331764501582
2402	410-419	0.45779544706292086
2402	420-429	0.4242519850651121
2402	430-439	0.4964118560587991
2402	440-449	0.41799820443123537
2402	450-459	0.4321190904202723
2402	460-469	-1.5432987401829195
2402	470-479	25.37096774193548
2402	480-489	-1.096774193548387
2402	490	-0.6451612903225806
2404	1	0.2338281420817978
2404	2	0.1188885366225918
2404	3	-0.08069081000177647
2404	4	0.21533035731346928
2404	5	0.3900103700740303
2404	6	0.31637798357807156
2404	7	0.1910542704237912
2404	8	0.047449265246243044
2404	9	0.3828241187386183
2404	10-19	0.22468924323960948
2404	20-29	0.08518052551433186
2404	30-39	0.20895186610227512
2404	40-49	0.12856286075000867
2404	50-59	0.16500900618748204
2404	60-69	0.21032123764724275
2404	70-79	0.2559965128441277
2404	80-89	0.23509720888179686
2404	90-99	0.3083062961439822
2404	100-109	0.25569150069470936
2404	110-119	0.3871363256200695
2404	120-129	0.2296916411629084
2404	130-139	0.3765357340417239
2404	140-149	0.295880226595564
2404	150-159	0.45709457350564975
2404	160-169	0.4072551743073376
2404	170-179	0.6933709496696778
2404	180-189	0.6620153955403509
2404	190-199	0.610657024427347
2404	200-209	0.5768641068857292
2404	210-219	0.5844063627607454
2404	220-229	0.5226235806491957
2404	230-239	0.5145173729665657
2404	240-249	0.5122281405840567
2404	250-259	0.5301168404771701
2404	260-269	0.4708882589810699
2404	270-279	0.487192065753284
2404	280-289	0.5122749726949394
2404	290-299	0.4930696297126289
2404	300-309	1.618620802004397
2404	310-319	1.803073842946354
2404	320-329	1.6222244505323111
2404	330-339	1.4795457057541164
2404	340-349	1.4107327261315952
2404	350-359	1.2526204540990094
2404	360-369	1.4010875726854408
2404	370-379	1.1102199843591194
2404	380-389	1.0305017053554835
2404	390-399	1.0947712666842335
2404	400-409	0.8316807648745979
2404	410-419	0.6464706116625294
2404	420-429	0.6421837719277619
2404	430-439	0.710679526054875
2404	440-449	0.5679045639632605
2404	450-459	0.552540454004621
2404	460-469	-0.11903688789394096
2404	470-479	-3.0290322580645164
2404	480-489	-1.096774193548387
2404	490	-0.6451612903225806
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	28.0
19	51.0
20	106.0
21	150.0
22	205.0
23	264.0
24	295.0
25	433.0
26	552.0
27	726.0
28	942.0
29	1258.0
30	1808.0
31	3877.0
32	13727.0
33	69684.0
34	1648.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.09503122454520771	0.04490486434553771	0.10025272039934001	99.75981119070991
2	0.025064227081897362	0.12114376422917059	0.2767508406959501	99.57704116799299
3	0.005221495854132292	0.26420769021909396	99.65329267528563	0.07727813864115793
4	0.08354393366611668	99.68357735123958	0.03550617180809958	0.19737254328620063
5	0.17753085904049792	0.03237327429562021	0.23392301426512666	99.55617285239875
6	99.75563399402661	0.07623383947033147	0.027151778441487917	0.1409803880615719
7	99.88721568955074	0.05325925771214937	0.04490486434553771	0.014620188391570416
8	99.82560203847198	0.06892374527454626	0.0553478560538023	0.050126360199670005
9	30.469516907203577	21.842561457006205	25.431817707136737	22.25610392865348
10-19	42.095281856346205	23.158900561832954	14.567660143277847	20.178157438542993
20-29	31.70314751770087	21.549739969506465	30.456045447899914	16.29106706489275
30-39	25.681196349130097	36.12418805739468	10.120094404645043	28.074521188830175
40-49	28.994131038659955	35.97172037845402	17.40324568182293	17.6309029010631
50-59	37.87599991645607	29.692662754025772	9.555232983145011	22.87610434637315
60-69	40.179619457382145	11.088368595835336	18.4607030222018	30.271308924580715
70-79	44.3266358946511	39.07683953298941	13.675828651392049	2.9206959209674386
80-89	30.639319952379957	5.148081622423192	40.77069278806993	23.441905637126926
90-99	17.68980137429771	30.10766724451221	31.96641533866622	20.236116042523864
100-109	38.77514150253765	27.931138912675703	28.74088848973454	4.5528310950521105
110-119	33.8434386683097	51.21525094509075	11.384740700515884	3.5565696860836695
120-129	29.817143215188285	29.28120888072015	27.71047849788007	13.191169406211491
130-139	22.49023580275277	17.806345161761943	38.45151318949853	21.25190584598676
140-149	27.85521836295662	28.71498987029804	27.750788445873976	15.679003320871363
150-159	15.76453142296205	46.34317759351699	16.242402723532237	21.64988825998872
160-169	30.92159401825435	20.00355061718081	18.462582760709285	30.61227260385555
170-179	25.26514755947284	22.27730320182126	18.647841433613902	33.809707805092
180-189	38.16976127320955	20.26629628856075	5.830322270724117	35.733620167505585
190-199	20.936945216065496	36.579920215543346	29.35890473902964	13.124229829361516
200-209	48.05972206702598	20.542331675315303	14.181850080777469	17.216096176881248
210-219	41.028843543098226	13.201925687671004	27.184465005534786	18.584765763695984
220-229	33.13366312335588	11.438638483091212	43.857478077526764	11.570220316026145
230-239	38.31314354936402	37.894484011779696	12.855740512542033	10.93663192631425
240-249	44.08200263372526	18.498003820050357	13.821000669396469	23.598992876827918
250-259	18.102574117219504	37.10868436909639	24.838993662088765	19.94974785159534
260-269	30.339188370684433	35.84744877712567	26.661479980784897	7.151882871405
270-279	28.212681969130514	7.532947638839575	26.62179661229349	37.63257377973642
280-289	27.829006453768873	36.74460619478268	10.716180371352785	24.71020698009566
290-299	30.136385471709936	25.42993796862925	23.60596503686376	20.827711522797053
300-309	25.671938286330203	34.246240550459866	29.083590438809594	10.998230724400337
310-319	28.390740485412145	18.19647952937834	33.109491399372445	20.303288585837063
320-329	49.510288332368816	42.44822406398008	5.07281953999695	2.9686680636541602
330-339	29.876591961605925	30.964521625396586	21.058657732564033	18.100228680433457
340-349	31.970386839152393	23.32600596358743	28.25212977802667	16.45147741923351
350-359	43.210404124202526	25.15531584545525	17.520860281564172	14.113419748778055
360-369	27.098951073065418	36.2606036920389	31.770427324348105	4.870017910547578
370-379	29.73383807812253	41.462881708019566	6.4267142556948444	22.376565958163056
380-389	36.80702880677369	29.948442451801377	12.132700827158311	21.111827914266627
390-399	40.06456916242062	4.6648971327694255	19.46183998058951	35.80869372422044
400-409	32.064006693510436	23.05862050933431	25.46598337081002	19.411389426345238
410-419	32.32888776392844	24.534480792985548	16.438217184072933	26.698414259013077
420-429	37.51987647295126	31.798695266470272	13.57123727905731	17.110190981521157
430-439	37.23666654457741	19.727915360747605	20.803172925485434	22.232245169189554
440-449	29.082385689181095	38.88684419109731	20.425149196643122	11.605620923078469
450-459	16.765331135596067	33.101786014294994	16.718765915846596	33.414116934262346
460-469	32.6986939715584	16.873253644471028	33.54743391746043	16.880618466510143
470-479	29.943502824858758	22.033898305084744	31.07344632768362	16.94915254237288
480-489	24.691358024691358	16.049382716049383	28.39506172839506	30.864197530864196
490-499	12.5	6.25	18.75	62.5
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.25
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.2
37	0.6000000000000001
38	0.0
39	0.0
40	0.6000000000000001
41	0.0
42	0.0
43	4.3500000000000005
44	2.9
45	0.45
46	1.65
47	16.149999999999977
48	50.95000000000019
49	266.6999999999935
50	7612.599999995272
51	7451.099999995417
52	2008.6500000003196
53	912.7000000000695
54	2161.950000000224
55	1731.1000000002564
56	1132.8500000001202
57	137.65000000000072
58	60.70000000000033
59	15.449999999999978
60	2.7000000000000006
61	2.05
62	1.0
63	1.5999999999999999
64	5.200000000000002
65	1.0
66	0.2
67	0.0
68	0.2
69	0.0
70	0.0
71	0.0
72	0.0
73	0.25
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004177196683305833
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0011487290879091043
210-219	0.0
220-229	1.0442991708264584E-4
230-239	0.0
240-249	1.0442991708264584E-4
250-259	9.398692537438126E-4
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	1.0447759634662742E-4
350-359	0.0
360-369	1.0449550094620677E-4
370-379	0.0
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-489	0.0
490-499	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
300-304	14.0
305-309	2.0
310-314	4.0
315-319	5.0
320-324	2.0
325-329	6.0
330-334	3.0
335-339	5.0
340-344	2.0
345-349	5.0
350-354	3.0
355-359	0.0
360-364	0.0
365-369	48.0
370-374	8.0
375-379	4.0
380-384	5.0
385-389	12.0
390-394	14.0
395-399	1.0
400-404	0.0
405-409	1.0
410-414	7.0
415-419	10.0
420-424	5.0
425-429	10.0
430-434	5.0
435-439	3290.0
440-444	16296.0
445-449	157.0
450-454	268.0
455-459	18.0
460-464	4670.0
465-469	70852.0
470-474	12.0
475-479	4.0
480-484	2.0
485-489	1.0
490-494	6.0
495-499	1.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.33054157354999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.86473111184427	3.7897616909292173
2	13.229957109864731	1.6750558700056393
3	6.483008907951171	1.2312287224043945
4	3.95908940943583	1.0025272039934001
5	2.78785879247773	0.8824327993483573
6	1.9465522929726162	0.7393638129451325
7	1.5176509402837348	0.6725286660122393
8	1.1052457934675024	0.5597443555629817
9	1.0392609699769053	0.592117629858602
>10	6.647970966677665	7.799870506902817
>50	0.41240514681623225	1.9507508511038243
>100	0.5278785879247773	8.970529877399278
>500	0.2144506763444408	8.998725955011592
>1k	0.2474430880897394	55.03456630255435
>5k	0.01649620587264929	6.10079575596817
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	5842	6.10079575596817	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	4763	4.973996950646422	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	4683	4.890453016980305	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	4520	4.720232252135592	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	4458	4.655485703544351	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4001	4.17824098247666	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3926	4.099918544664676	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3921	4.094697048810543	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3118	3.2561248146368973	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3103	3.2404603270745	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3041	3.17571377848326	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2979	3.1109672298920197	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	2878	3.005493013638547	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	2682	2.8008103761565613	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	2607	2.722487938344577	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	2020	2.109484325069446	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	993	1.036989076630673	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	755	0.7884458739739761	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	734	0.7665155913866205	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	731	0.763382693874141	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	706	0.7372752146034796	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	685	0.7153449320161239	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	647	0.6756615635247185	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	627	0.6547755801081894	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	582	0.6077821174209987	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	559	0.5837632364919902	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	555	0.5795860398086844	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	523	0.5461684663422377	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	520	0.5430355688297583	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	476	0.4970864053133942	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	465	0.4855991144343031	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	440	0.4594916351636417	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	437	0.4563587376511623	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	433	0.45218154096785645	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	433	0.45218154096785645	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	420	0.4386056517471125	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	404	0.4218968650138892	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	395	0.41249817247645104	No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	345	0.36028321393512813	No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	340	0.35506171808099585	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	332	0.34670732471438415	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	331	0.3456630255435577	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	321	0.33522003383529314	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	290	0.30284675953967294	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	277	0.28927087031892895	No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	275	0.287182271977276	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	271	0.28300507529397023	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	204	0.2130370308485975	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	184	0.19215104743206834	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	167	0.17439796152801854	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	156	0.1629106706489275	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	143	0.14933478142818354	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	131	0.13680319137826605	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	127	0.1326259946949602	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	125	0.13053739635330727	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	117	0.12218300298669563	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	117	0.12218300298669563	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	114	0.11905010547421625	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	111	0.11591720796173689	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	105	0.10965141293677813	No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	104	0.10860711376595167	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	100	0.10442991708264583	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	100	0.10442991708264583	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	96	0.10025272039934001	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0
310-319	5.221495854132291E-4	0.0	0.0	0.0	0.0
320-329	0.0010442991708264585	0.0	0.0	0.0	0.0
330-339	0.0010442991708264585	0.005117065937049646	0.0	0.0	0.0
340-349	0.0010442991708264585	0.005221495854132291	0.0	0.0	0.0
350-359	0.0010442991708264585	0.005221495854132291	0.0	0.0	0.0
360-369	0.0010442991708264585	0.005221495854132291	0.0	0.0	0.0
370-379	0.0010442991708264585	0.005221495854132291	0.0	0.0	0.0
380-389	0.0010442991708264585	0.005221495854132291	0.0	0.0	0.0
390-399	0.0010442991708264585	0.005221495854132291	0.0	0.0	0.0
400-409	0.0010442991708264585	0.005221495854132291	0.0	0.0	0.0
410-419	0.0010442991708264585	0.005221495854132291	0.0	0.0	0.0
420-429	0.0010442991708264585	0.005221495854132291	0.0	0.0	0.0
430-439	0.0010442991708264585	0.005221495854132291	0.0	0.0	0.0
440-449	0.0010442991708264585	0.005221495854132291	0.0	0.0	0.0
450-459	0.0010442991708264585	0.005221495854132291	0.0	0.0	0.0
460-469	0.0010442991708264585	0.005221495854132291	0.0	0.0	0.0
470-479	0.0010442991708264585	0.005221495854132291	0.0	0.0	0.0
480-488	0.0010442991708264583	0.005221495854132291	0.0	0.0	0.0
>>END_MODULE
