##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	DCFS2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	64901
Sequences flagged as poor quality	0
Sequence length	300-499
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26065854147085	34.0	34.0	34.0	20.0	34.0
2	33.1275635198225	34.0	34.0	34.0	34.0	34.0
3	33.588604181753745	34.0	34.0	34.0	34.0	34.0
4	33.434908553026915	34.0	34.0	34.0	34.0	34.0
5	33.28044252014607	34.0	34.0	34.0	34.0	34.0
6	33.080168256267235	34.0	34.0	34.0	34.0	34.0
7	33.5128426372475	34.0	34.0	34.0	34.0	34.0
8	33.500223417204666	34.0	34.0	34.0	34.0	34.0
9	33.44637216683872	34.0	34.0	34.0	34.0	34.0
10-19	33.41191507064606	34.0	34.0	34.0	34.0	34.0
20-29	33.71595352922143	34.0	34.0	34.0	34.0	34.0
30-39	33.619471194588684	34.0	34.0	34.0	34.0	34.0
40-49	33.53476833947089	34.0	34.0	34.0	34.0	34.0
50-59	33.51948043943854	34.0	34.0	34.0	34.0	34.0
60-69	33.46966148441472	34.0	34.0	34.0	32.6	34.0
70-79	33.53242014760943	34.0	34.0	34.0	34.0	34.0
80-89	33.65129042695798	34.0	34.0	34.0	34.0	34.0
90-99	33.26130105853531	34.0	34.0	34.0	34.0	34.0
100-109	33.426922543566356	34.0	34.0	34.0	34.0	34.0
110-119	33.258302645567866	34.0	34.0	34.0	34.0	34.0
120-129	33.39541763609189	34.0	34.0	34.0	34.0	34.0
130-139	33.23796859832668	34.0	34.0	34.0	34.0	34.0
140-149	33.40724025823947	34.0	34.0	34.0	34.0	34.0
150-159	33.16700050846674	34.0	34.0	34.0	34.0	34.0
160-169	33.2355017642255	34.0	34.0	34.0	34.0	34.0
170-179	33.04613488235928	34.0	34.0	34.0	33.1	34.0
180-189	33.059230212169304	34.0	34.0	34.0	34.0	34.0
190-199	33.09484445540131	34.0	34.0	34.0	34.0	34.0
200-209	33.18962265604536	34.0	34.0	34.0	34.0	34.0
210-219	33.24046624859402	34.0	34.0	34.0	34.0	34.0
220-229	33.267478159042234	34.0	34.0	34.0	34.0	34.0
230-239	33.33770666091432	34.0	34.0	34.0	34.0	34.0
240-249	33.323737692793635	34.0	34.0	34.0	34.0	34.0
250-259	33.24727508050724	34.0	34.0	34.0	33.8	34.0
260-269	33.39361951279641	34.0	34.0	34.0	34.0	34.0
270-279	33.362515215482034	34.0	34.0	34.0	34.0	34.0
280-289	33.33516432720605	34.0	34.0	34.0	34.0	34.0
290-299	33.27265373414893	34.0	34.0	34.0	34.0	34.0
300-309	31.71652516896238	34.0	34.0	34.0	22.8	34.0
310-319	31.305639323191635	34.0	34.0	34.0	20.0	34.0
320-329	31.76157226658854	34.0	34.0	34.0	20.0	34.0
330-339	31.947838401062047	34.0	34.0	34.0	22.8	34.0
340-349	31.972732818468785	34.0	34.0	34.0	20.0	34.0
350-359	32.18718716069293	34.0	34.0	34.0	24.2	34.0
360-369	31.978128929025097	34.0	34.0	34.0	22.8	34.0
370-379	32.22839788869961	34.0	34.0	34.0	27.0	34.0
380-389	32.492484303701715	34.0	34.0	34.0	32.6	34.0
390-399	32.251878962443385	34.0	34.0	34.0	31.2	34.0
400-409	32.63933529581819	34.0	34.0	34.0	32.6	34.0
410-419	32.94139426279601	34.0	34.0	34.0	34.0	34.0
420-429	32.85593403014012	34.0	34.0	34.0	34.0	34.0
430-439	32.839707165985814	34.0	34.0	34.0	32.6	34.0
440-449	32.998993416634065	34.0	34.0	34.0	34.0	34.0
450-459	32.989010184365554	34.0	34.0	34.0	34.0	34.0
460-469	32.34470424508398	34.0	34.0	34.0	30.0	34.0
470-479	32.46241471571906	NaN	NaN	NaN	NaN	NaN
480-489	32.77970760233918	NaN	NaN	NaN	NaN	NaN
490-499	33.160000000000004	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.16320138153515984
1101	2	-0.4747277655440314
1101	3	-0.2380588053086541
1101	4	0.017479486272087286
1101	5	-0.016298349807954082
1101	6	-0.09745323047252441
1101	7	-0.020360649754117333
1101	8	-0.08513059090580555
1101	9	-0.01299148336870104
1101	10-19	-0.1636421409955986
1101	20-29	-0.028770810653888645
1101	30-39	-0.13345792209826612
1101	40-49	-0.20666530898380842
1101	50-59	-0.2130451740453978
1101	60-69	-0.21477238565196188
1101	70-79	-0.15690607239507415
1101	80-89	-0.0808225261133586
1101	90-99	-0.46094370754934744
1101	100-109	-0.25969259283142065
1101	110-119	-0.2678333393893766
1101	120-129	-0.28638898542045865
1101	130-139	-0.2364368915335291
1101	140-149	-0.11688183951084596
1101	150-159	-0.3431896741209286
1101	160-169	-0.3268774949019999
1101	170-179	-0.44222558841643433
1101	180-189	-0.6081709827776152
1101	190-199	-0.5659842452547039
1101	200-209	-0.602754471816958
1101	210-219	-0.5117892298778202
1101	220-229	-0.4006687002327496
1101	230-239	-0.5696502295035302
1101	240-249	-0.5736325846580641
1101	250-259	-0.5345570545662852
1101	260-269	-0.3969011683251651
1101	270-279	-0.4348626292904143
1101	280-289	-0.6102492334840335
1101	290-299	-0.4725704305985303
1101	300-309	-1.5405994610577665
1101	310-319	-1.7642439798476879
1101	320-329	-1.5266721556481784
1101	330-339	-1.212858810578954
1101	340-349	-1.3334259766188623
1101	350-359	-1.1120454922201013
1101	360-369	-1.2643112761301367
1101	370-379	-1.2237982096400124
1101	380-389	-0.935699960855306
1101	390-399	-1.1511786097237362
1101	400-409	-1.0479272738742296
1101	410-419	-0.6173016032728711
1101	420-429	-0.7617931849678357
1101	430-439	-0.5805438847585691
1101	440-449	-0.5770003772880017
1101	450-459	-0.6118773359624115
1101	460-469	-0.48199564499497427
1101	470-479	-1.096774193548387
1101	480-486	-1.096774193548387
1102	1	-0.24290255289470508
1102	2	-0.17916756334747674
1102	3	-0.13907181815909553
1102	4	-0.2885965607447005
1102	5	-0.16601718291460088
1102	6	0.009349587286664018
1102	7	-0.49395938298053466
1102	8	0.07023188092675525
1102	9	-0.010311770016826927
1102	10-19	-0.08041877064703584
1102	20-29	-0.08735705885218437
1102	30-39	0.03927193437466059
1102	40-49	-0.14070781898198703
1102	50-59	-0.10843078885690005
1102	60-69	-0.05710576635397757
1102	70-79	-0.17424706591910422
1102	80-89	-0.11800557895649177
1102	90-99	-0.19078393979118147
1102	100-109	-0.16637360543825253
1102	110-119	-0.11530689752088108
1102	120-129	-0.24984946979894573
1102	130-139	-0.28635061288395036
1102	140-149	-0.2629526083043672
1102	150-159	-0.4136255335577772
1102	160-169	-0.5367132609549117
1102	170-179	-0.7554211465885459
1102	180-189	-0.46787986846346996
1102	190-199	-0.6914740034714768
1102	200-209	-0.4964104534649749
1102	210-219	-0.5921806304340649
1102	220-229	-0.6271795916434542
1102	230-239	-0.4417142178102367
1102	240-249	-0.3579562615495817
1102	250-259	-0.5919658529584524
1102	260-269	-0.5661555177841535
1102	270-279	-0.4675652826126466
1102	280-289	-0.7652056881420606
1102	290-299	-0.709099795790678
1102	300-309	-1.3591970777369617
1102	310-319	-1.63357141239808
1102	320-329	-1.3563459208484439
1102	330-339	-0.9372259313081521
1102	340-349	-0.9463738643145128
1102	350-359	-0.9287388883507575
1102	360-369	-1.0869304929230097
1102	370-379	-0.7671527235359505
1102	380-389	-0.7850980403940682
1102	390-399	-0.8359123639615333
1102	400-409	-0.6543849770350718
1102	410-419	-0.493172002590768
1102	420-429	-0.5847473659297009
1102	430-439	-0.4923346235068138
1102	440-449	-0.22455673819828093
1102	450-459	-0.5687927067758167
1102	460-469	-0.3378902785143012
1102	470-479	-1.096774193548387
1102	480-486	-1.096774193548387
1103	1	0.05958326773627931
1103	2	-0.5008841532092276
1103	3	-0.02838411876172131
1103	4	0.03529124499794278
1103	5	-0.07028428677847387
1103	6	-0.1583436066162065
1103	7	-0.20769121566398496
1103	8	-0.37380392198002
1103	9	0.019243352280227555
1103	10-19	-0.1719931237133281
1103	20-29	-0.034179993981567236
1103	30-39	0.007246500131486755
1103	40-49	-0.1105389958696179
1103	50-59	0.02297651351815233
1103	60-69	-0.04671494749540983
1103	70-79	-0.08820266676985966
1103	80-89	-0.11260412557116695
1103	90-99	-0.08004528328638827
1103	100-109	-0.15192615772467377
1103	110-119	-0.39967635870485196
1103	120-129	-0.15881570517647248
1103	130-139	-0.16234759976804014
1103	140-149	-0.073684618229926
1103	150-159	-0.09905844741810199
1103	160-169	-0.1932490638616926
1103	170-179	-0.4111704376200862
1103	180-189	-0.3388343172405044
1103	190-199	-0.414010338104589
1103	200-209	-0.2606119777471534
1103	210-219	-0.30623814549964123
1103	220-229	-0.16220927969733623
1103	230-239	-0.2929770678634114
1103	240-249	-0.23938571918873208
1103	250-259	-0.0813822833023039
1103	260-269	-0.16456255429602606
1103	270-279	-0.11396505219790498
1103	280-289	-0.1702903212508815
1103	290-299	-0.2171908982366162
1103	300-309	-0.37280040988867924
1103	310-319	-0.6026271550425335
1103	320-329	-0.5889052969562094
1103	330-339	-0.3423615215243956
1103	340-349	-0.35591835208480305
1103	350-359	-0.6522987961848514
1103	360-369	-0.31232042557100925
1103	370-379	-0.2658898734827737
1103	380-389	-0.015300096402583563
1103	390-399	-0.327167681224914
1103	400-409	-0.19789126913006783
1103	410-419	-0.22629855345924454
1103	420-429	-0.1145528427109781
1103	430-439	-0.06997331546002528
1103	440-449	-0.23675636043481774
1103	450-459	-0.2041796886410978
1103	460-469	0.09635911447038126
1103	470-479	-1.096774193548387
1103	480-486	-1.096774193548387
1104	1	0.27850915891826844
1104	2	-0.19651423451004035
1104	3	-0.11859675165727879
1104	4	0.18534596669900338
1104	5	0.04154341803703687
1104	6	-0.13539957909900835
1104	7	-0.13432504931000722
1104	8	-0.1459271239812665
1104	9	0.08576930600130339
1104	10-19	0.055207470408007
1104	20-29	-0.0802947532060827
1104	30-39	-0.0016145067478205988
1104	40-49	-0.15989406153565255
1104	50-59	-0.09390903995277
1104	60-69	-0.11540631710141014
1104	70-79	-0.044265049509064625
1104	80-89	-0.11004784039293014
1104	90-99	-0.1805651394464931
1104	100-109	-0.16463331845012874
1104	110-119	-0.12708802224456406
1104	120-129	-0.08897127115521641
1104	130-139	-0.18973190245721128
1104	140-149	-0.19934127864268447
1104	150-159	-0.35692664629240767
1104	160-169	-0.4345795829360952
1104	170-179	-0.7940933581954539
1104	180-189	-0.8395613341260599
1104	190-199	-0.7863524269100921
1104	200-209	-0.5131838976971181
1104	210-219	-0.45022266707562864
1104	220-229	-0.3831872346691938
1104	230-239	-0.512528349914696
1104	240-249	-0.4948657066809048
1104	250-259	-0.41935523514720785
1104	260-269	-0.5230248744961514
1104	270-279	-0.5557841578889651
1104	280-289	-0.5399705901689629
1104	290-299	-0.611671823815108
1104	300-309	-1.8471124799324556
1104	310-319	-1.8756923520406623
1104	320-329	-1.9736457597454482
1104	330-339	-1.6102711525437776
1104	340-349	-1.693269821753347
1104	350-359	-1.530042698623884
1104	360-369	-1.3668076050581917
1104	370-379	-1.5508101361469429
1104	380-389	-0.716363260880378
1104	390-399	-0.9328626468284185
1104	400-409	-0.6573073104058764
1104	410-419	-0.5853041819770723
1104	420-429	-0.8228744499655178
1104	430-439	-0.7467986750597717
1104	440-449	-0.6056303522011675
1104	450-459	-0.5886147359238052
1104	460-469	-0.603938609677499
1104	470-479	-1.096774193548387
1104	480-486	-1.096774193548387
1201	1	-0.33795136587655605
1201	2	-0.07464007737984701
1201	3	-0.07422808522179736
1201	4	-0.2084428053119396
1201	5	0.045396537136944914
1201	6	-0.15772840428299162
1201	7	0.04014418353397531
1201	8	-0.05886589791877128
1201	9	0.09283977954964229
1201	10-19	0.08846759447844477
1201	20-29	0.051697694680484574
1201	30-39	0.012380021823069853
1201	40-49	-0.07301393207085027
1201	50-59	-0.0217555316878304
1201	60-69	-0.1908773609113794
1201	70-79	-0.0680946645824747
1201	80-89	-0.059163549559499984
1201	90-99	-0.26445332193021187
1201	100-109	-0.09684732068493673
1201	110-119	-0.27249125115837103
1201	120-129	-0.14010216161103273
1201	130-139	-0.18368828514900315
1201	140-149	-0.03240682019856678
1201	150-159	-0.17340451012317004
1201	160-169	-0.3155740729757852
1201	170-179	-0.3573037770294931
1201	180-189	-0.31087678898676785
1201	190-199	-0.35304606541046013
1201	200-209	-0.3292822292965454
1201	210-219	-0.3293811458642111
1201	220-229	-0.3314013139663885
1201	230-239	-0.2600796927266984
1201	240-249	-0.21447230322080912
1201	250-259	-0.24835958917178402
1201	260-269	-0.14156993672402507
1201	270-279	-0.1833667109656787
1201	280-289	-0.3059880062672917
1201	290-299	-0.2752386561658966
1201	300-309	-1.316320278645506
1201	310-319	-1.6299808735988606
1201	320-329	-1.1586282280535443
1201	330-339	-0.8011065087646436
1201	340-349	-1.0592814581730288
1201	350-359	-0.7644433459478925
1201	360-369	-1.1268392006348122
1201	370-379	-0.8326735045436564
1201	380-389	-0.6765104500299906
1201	390-399	-0.7265319721472636
1201	400-409	-0.7643353996441142
1201	410-419	-0.388239598196499
1201	420-429	-0.5794017438098749
1201	430-439	-0.7046848539215631
1201	440-449	-0.5585060788194056
1201	450-459	-0.39335200164761375
1201	460-469	-1.2085888100014905
1201	470-479	-1.096774193548387
1201	480-486	-1.096774193548387
1202	1	0.4607584814114887
1202	2	-0.28560637543144907
1202	3	-0.2064467968244017
1202	4	0.054700076906776474
1202	5	-0.10536263435682258
1202	6	0.27844414267154605
1202	7	0.2604355649627976
1202	8	-0.16297771115380755
1202	9	0.0890439220807977
1202	10-19	-0.10107075904095097
1202	20-29	0.056810034508458784
1202	30-39	-0.04512173348675219
1202	40-49	-0.06247987906049701
1202	50-59	-0.1166958511542191
1202	60-69	0.047658271877814684
1202	70-79	-0.08551392033110972
1202	80-89	-0.019691020158063566
1202	90-99	-0.13063289012399792
1202	100-109	-0.030362767411290292
1202	110-119	-0.006638609417102259
1202	120-129	-0.15235202996279895
1202	130-139	-0.044790619711058355
1202	140-149	-0.06955356409886804
1202	150-159	-0.20908337619303552
1202	160-169	-0.35603396414658306
1202	170-179	-0.5878264205260777
1202	180-189	-0.5530437189499153
1202	190-199	-0.49007871417296656
1202	200-209	-0.3297715219067001
1202	210-219	-0.5432217637332641
1202	220-229	-0.3680675418056012
1202	230-239	-0.19910242398877642
1202	240-249	-0.3260310183340138
1202	250-259	-0.2416564998265187
1202	260-269	-0.36871141469487867
1202	270-279	-0.3253076447905059
1202	280-289	-0.3463052785158496
1202	290-299	-0.3818276503733742
1202	300-309	-1.4290682161564838
1202	310-319	-1.393634989800372
1202	320-329	-1.1751588582097696
1202	330-339	-1.268536735199607
1202	340-349	-1.1031049617714146
1202	350-359	-0.9078543517404114
1202	360-369	-0.8788446420952241
1202	370-379	-0.7732830358759379
1202	380-389	-0.8932965185850428
1202	390-399	-0.6838514916005849
1202	400-409	-0.7201206966792704
1202	410-419	-0.48137905435370243
1202	420-429	-0.5406642022816399
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2402	280-289	0.4403887341496713
2402	290-299	0.41514270080682536
2402	300-309	1.2666475773572365
2402	310-319	1.6292804774803855
2402	320-329	1.3160568393530738
2402	330-339	1.288083875047942
2402	340-349	1.2422582122834704
2402	350-359	1.1368851416389845
2402	360-369	1.1929505987414615
2402	370-379	1.0960620994147945
2402	380-389	0.790797950628118
2402	390-399	1.0698754634542382
2402	400-409	0.691220428757525
2402	410-419	0.6499668909409095
2402	420-429	0.5869343035624297
2402	430-439	0.5161441616224849
2402	440-449	0.5528225939682798
2402	450-459	0.6681864598908547
2402	460-469	0.7667631566727309
2402	470-479	-1.096774193548387
2402	480-486	-1.096774193548387
2404	1	0.7719637544811704
2404	2	-0.007593192757248346
2404	3	-0.18494648295120442
2404	4	0.25989467828781443
2404	5	-0.3238968465351064
2404	6	0.36515161285359454
2404	7	-0.20033028563356226
2404	8	0.16101789462145888
2404	9	0.16727681599166289
2404	10-19	0.11374405577385005
2404	20-29	-0.031968998762096135
2404	30-39	0.025931311083247977
2404	40-49	0.0880458585415127
2404	50-59	0.09829159391797049
2404	60-69	0.05171508292615101
2404	70-79	0.1949867074152749
2404	80-89	-0.020051974332581324
2404	90-99	0.15783226995133504
2404	100-109	0.16150007000238986
2404	110-119	0.29482874777059465
2404	120-129	0.1375961809599815
2404	130-139	0.2316061160077112
2404	140-149	0.2097464986757771
2404	150-159	0.33577695023509335
2404	160-169	0.2981405493499736
2404	170-179	0.40513497307091484
2404	180-189	0.32751183660563754
2404	190-199	0.5257875758458681
2404	200-209	0.4101453016965664
2404	210-219	0.458127891006221
2404	220-229	0.4108323326451213
2404	230-239	0.304420802627682
2404	240-249	0.29477877450967327
2404	250-259	0.49319127167379406
2404	260-269	0.31190691549971916
2404	270-279	0.23698833763260296
2404	280-289	0.27433973090035124
2404	290-299	0.3803427827716419
2404	300-309	1.3312739056263148
2404	310-319	1.5079579166654185
2404	320-329	1.2123332949603522
2404	330-339	1.1965291781713852
2404	340-349	1.0595205875066789
2404	350-359	1.0138735201993185
2404	360-369	1.0438203206883756
2404	370-379	0.862709027616134
2404	380-389	0.6765240794216041
2404	390-399	0.7912581429509018
2404	400-409	0.6039191704303661
2404	410-419	0.5025146333539396
2404	420-429	0.5377411133329772
2404	430-439	0.575315146078438
2404	440-449	0.500555123143755
2404	450-459	0.4710319883461409
2404	460-469	0.5417418310708157
2404	470-479	-1.096774193548387
2404	480-486	-1.096774193548387
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	9.0
19	43.0
20	69.0
21	119.0
22	133.0
23	195.0
24	227.0
25	291.0
26	367.0
27	511.0
28	653.0
29	791.0
30	1304.0
31	2562.0
32	9464.0
33	47038.0
34	1124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.10631577325464939	0.046224249241151906	0.11093819817876457	99.73652177932544
2	0.032357473035439135	0.12172573189522343	0.26040061633281975	99.58551617873653
3	0.023112124620575953	0.23882528774595152	99.64869570576724	0.08936688186622703
4	0.08782607355818862	99.6949199550084	0.049305865857228695	0.16794810557618525
5	0.15254002249580131	0.03081616616076794	0.22341720466556753	99.59322660667786
6	99.73960339594151	0.06779556555368947	0.027734549544691144	0.16486648896010847
7	99.88135776028105	0.053928290781343896	0.0508466741652671	0.013867274772345572
8	99.84900078581224	0.06933637386172786	0.046224249241151906	0.03543859108488313
9	29.734518728524982	23.07822683779911	25.71146823623673	21.475786197439177
10-19	40.90661160844979	24.101015392674995	14.899000015408085	20.093372983467127
20-29	30.470871018936535	20.54328900941434	30.13805642440024	18.847783547248888
30-39	23.254649389069506	37.2393337544876	10.102925994976966	29.403090861465923
40-49	29.771652208748712	33.405032279934055	17.887859971340966	18.93545553997627
50-59	37.99047780465632	31.01385186668926	10.0040061016009	20.991664227053512
60-69	40.1685644288994	10.434970185359239	19.46318238547942	29.93328300026194
70-79	44.25879416341813	39.2456202523844	15.125498836689728	1.3700867475077425
80-89	30.307083095792052	5.940124189149628	39.82835395448453	23.924438760573796
90-99	19.287838400024654	29.634828430994897	30.84004868954253	20.237284479437914
100-109	39.639450855919016	25.3048489237454	33.10149304325049	1.9542071770850988
110-119	36.50390594906088	47.91467003590083	11.777938706645505	3.803485308392783
120-129	29.964869570576724	30.91292892251275	27.929153633996396	11.19304787291413
130-139	21.903206422089028	14.10271028181384	42.35050307391258	21.643580222184557
140-149	29.20987349963791	28.181075792360673	29.180289980123575	13.428760727877846
150-159	12.375156006841188	53.29224511178564	10.074729202939862	24.257869678433305
160-169	30.209134233885816	20.1835413684556	19.42176456720939	30.185559830449193
170-179	21.974854008412812	20.955609312645414	19.314494383753715	37.755042295188055
180-189	39.410024498852096	19.901542349116347	2.29333908568435	38.395094066347205
190-199	20.616785565707772	38.69370271644505	29.581824625198376	11.107687092648803
200-209	49.1865189730062	20.1762400982433	11.579797257631345	19.057443671119152
210-219	38.64886519468113	12.407821142971603	29.316035192061758	19.62727847028551
220-229	31.225713009044544	10.509545307468297	47.6644427666754	10.60029891681176
230-239	39.06226406372783	38.845780496448434	11.188271367159212	10.90368407266452
240-249	47.69880279194466	17.014375741513998	8.988613426603596	26.298208039937755
250-259	19.166386751432192	38.89316893834572	25.698067506309645	16.242376803912446
260-269	30.455462935856147	38.41111847275081	28.409885826104375	2.7235327652886703
270-279	29.45455385895441	2.770835580345449	24.874193001648663	42.900417559051476
280-289	27.924531209072278	40.17503582379316	8.59632363137702	23.304109335757538
290-299	31.01169473505801	23.348176453367437	21.49874424122895	24.141384570345604
300-309	20.674543739945637	32.762122248725674	33.6960608470011	12.867273164327584
310-319	29.697514188894523	15.694774264435072	34.586846205173806	20.020865341496602
320-329	48.661119527542795	46.81789530301466	2.1015222011426142	2.419462968299934
330-339	29.811547621800354	30.091798275024857	20.46985918098365	19.626794922191134
340-349	31.124694301125345	21.155749914419737	29.07446870846273	18.64508707599219
350-359	47.325359921653906	21.9859501391877	19.110895196674917	11.577794742483478
360-369	22.446665391285027	38.62801848891961	36.89607079602328	2.029245323772085
370-379	29.618559503728832	46.74816338887962	2.533874585256446	21.0994025221351
380-389	39.32833929774465	29.65733993436057	10.656363377896447	20.357957389998333
390-399	40.82827064879655	2.0020405323934516	19.871075453322824	37.29861336548718
400-409	30.471478598319408	21.348495272845337	28.007328442551348	20.172697686283907
410-419	31.349240096671394	21.482480242822703	18.641559730767387	28.526719929738515
420-429	38.84306339073634	30.5623082442597	11.408847980997624	19.185780384006335
430-439	39.310995460766954	19.92638245551629	20.27529249827942	20.487329585437337
440-449	29.56416142607604	39.805373748461506	20.11499447047166	10.51547035499079
450-459	16.780628683037577	33.15643623858571	16.699163647897432	33.363771430479275
460-469	32.71936463101391	16.878465706561034	33.53748941473694	16.86468024768812
470-479	25.71428571428571	31.428571428571427	26.53061224489796	16.3265306122449
480-489	25.405405405405407	18.37837837837838	26.486486486486488	29.72972972972973
490-499	19.35483870967742	6.451612903225806	19.35483870967742	54.83870967741935
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.2
36	1.9999999999999998
37	1.4
38	0.6000000000000001
39	0.2
40	0.0
41	0.2
42	1.15
43	2.4
44	4.0
45	1.15
46	1.9
47	1.7999999999999998
48	8.9
49	96.2000000000008
50	3565.7999999989506
51	6820.49999999599
52	1802.6500000002723
53	182.64999999999833
54	1681.1000000002432
55	1350.85000000017
56	393.0499999999863
57	48.25000000000015
58	22.299999999999958
59	7.350000000000004
60	4.400000000000001
61	4.450000000000001
62	0.8500000000000001
63	1.65
64	2.85
65	0.8
66	0.8
67	0.45
68	0.95
69	0.25
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0015408083080383967
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	1.540808308038397E-4
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0013867274772345571
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	6.163233232153588E-4
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	1.5410125068575055E-4
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	0.0
380-389	0.0
390-399	0.0
400-409	0.0
410-419	0.0
420-429	0.0
430-439	0.0
440-449	0.0
450-459	0.0
460-469	0.0
470-479	0.0
480-489	0.0
490-499	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
300-304	5.0
305-309	1.0
310-314	2.0
315-319	3.0
320-324	3.0
325-329	10.0
330-334	7.0
335-339	14.0
340-344	2.0
345-349	13.0
350-354	0.0
355-359	2.0
360-364	5.0
365-369	90.0
370-374	2.0
375-379	19.0
380-384	5.0
385-389	17.0
390-394	14.0
395-399	4.0
400-404	4.0
405-409	0.0
410-414	9.0
415-419	4.0
420-424	0.0
425-429	2.0
430-434	0.0
435-439	855.0
440-444	3851.0
445-449	46.0
450-454	8.0
455-459	15.0
460-464	3516.0
465-469	56345.0
470-474	4.0
475-479	3.0
480-484	2.0
485-489	4.0
490-494	13.0
495-499	2.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.565384200551609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.343111945552685	3.830449453783455
2	14.198544942501758	1.8643780527264602
3	7.650786200422437	1.5069105252615522
4	5.022295235860127	1.3189319116808678
5	3.2386763670499885	1.063157732546494
6	2.2764609246655714	0.8967504352783471
7	1.5489321755456464	0.7118534383137394
8	1.1499647969960103	0.6039968567510515
9	1.1030274583431119	0.651761914300242
>10	4.3417038253930995	4.4359871188425455
>50	0.23468669326449193	1.272707662439716
>100	0.32856137057028867	3.18176915609929
>500	0.09387467730579677	5.432890094143388
>1k	0.46937338652898386	73.22845564783286
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4208	6.483721360225575	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3717	5.727184480978721	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3505	5.400533119674581	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3342	5.149381365464323	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3315	5.107779541147286	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3118	4.804240304463722	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3029	4.667108365048304	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	2626	4.0461626169088305	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2611	4.023050492288254	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2289	3.5269102170998905	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2165	3.335849986903129	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2018	3.109351165621485	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1942	2.992249734210567	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1861	2.8674442612594566	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1735	2.673302414446619	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1623	2.5007318839463184	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1257	1.9367960432042648	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1112	1.7133788385386972	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1050	1.617848723440317	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1003	1.545430732962512	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	975	1.502288100337437	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	917	1.41292121847121	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	850	1.3096870618326375	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	784	1.207993713502103	No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	181	0.2788863037549499	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	178	0.27426387883083464	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	175	0.26964145390671945	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	163	0.2511517542102587	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	163	0.2511517542102587	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	158	0.24344771267006673	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	156	0.2403660960539899	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	145	0.22341720466556753	No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	132	0.2033866966610684	No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	129	0.1987642717369532	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	125	0.19260103850479962	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	124	0.19106023019676122	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	123	0.1895194218887228	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	113	0.17411133880833887	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	99	0.15254002249580131	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	93	0.1432951726475709	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	93	0.1432951726475709	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	88	0.13559113110737894	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	87	0.13405032279934054	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	84	0.12942789787522532	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	76	0.11710143141091818	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	75	0.11556062310287976	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	69	0.10631577325464939	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0
320-329	0.0	1.540808308038397E-4	0.0	0.0	0.0
330-339	0.0	0.00431426326250751	0.0	0.0	0.0
340-349	0.0	0.00462242492411519	0.0	0.0	0.0
350-359	0.0	0.00462242492411519	0.0	0.0	0.0
360-369	0.0	0.00462242492411519	0.0	0.0	0.0
370-379	0.0	0.00462242492411519	0.0	0.0	0.0
380-389	0.0	0.00462242492411519	0.0	0.0	0.0
390-399	0.0	0.00462242492411519	0.0	0.0	0.0
400-409	0.0	0.00462242492411519	0.0	0.0	0.0
410-419	0.0	0.00462242492411519	0.0	0.0	0.0
420-429	0.0	0.00462242492411519	0.0	0.0	0.0
430-439	0.0	0.00462242492411519	0.0	0.0	0.0
440-449	0.0	0.00462242492411519	0.0	0.0	0.0
450-459	0.0	0.00462242492411519	0.0	0.0	0.0
460-469	0.0	0.00462242492411519	0.0	0.0	0.0
470-479	0.0	0.00462242492411519	0.0	0.0	0.0
480-488	0.0	0.004622424924115191	0.0	0.0	0.0
>>END_MODULE
