FastQCFastQC Report
Tue 15 Oct 2024
DCFS1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDCFS1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116372
Sequences flagged as poor quality0
Sequence length302-490
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT64165.513353727700822No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT58375.015811363558244No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT52434.50537930086275No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT51314.409136218334307No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT50774.362733303543808No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT48844.1968858488296155No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT48184.140171175196783No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT47564.086893754511395No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT47144.050802598563228No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT46934.032757020589145No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT45583.916749733612897No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT43013.6959062317395937No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT41893.5996631492111506No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT39323.3788196473378473No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT35143.019626714329908No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT33142.8477640669576876No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT25722.2101536452067507No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT21691.8638504107517273No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT20961.801120544460867No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT20641.7736225208813117No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT20451.7572955693809507No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT18161.5605128381397586No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT17591.511531983638676No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT14011.2038978448424018No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC4570.392706149245523No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC3610.31021207850685734No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC3580.30763413879627405No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC3410.2930258137696353No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC3400.2921665005327742No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC3220.27669886226927437No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3180.27326160932183No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2870.24662289897913586No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2860.2457635857422748No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2790.23974839308424706No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC2790.23974839308424706No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2760.2371704533736638No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2420.20795380332038635No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2300.19764204447805314No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2260.19420479153060874No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2250.19334547829374765No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC2210.18990822534630322No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2000.17186264737222012No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1960.16842539442477572No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1800.1546763826349981No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1800.1546763826349981No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1780.1529577561612759No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1740.1495205032138315No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1330.1142886605025264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)