##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	JVMS2_cleaned_R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	116755
Sequences flagged as poor quality	0
Sequence length	15-301
%GC	52
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.211005952635862	34.0	34.0	34.0	20.0	34.0
2	30.480990107490044	34.0	34.0	34.0	20.0	34.0
3	32.01007237377414	34.0	34.0	34.0	20.0	34.0
4	32.860451372532225	34.0	34.0	34.0	34.0	34.0
5	31.841608496424136	34.0	34.0	34.0	20.0	34.0
6	32.3867157723438	34.0	34.0	34.0	34.0	34.0
7	31.905425891824763	34.0	34.0	34.0	20.0	34.0
8	31.997952978459168	34.0	34.0	34.0	20.0	34.0
9	32.48210355016916	34.0	34.0	34.0	34.0	34.0
10-14	32.58625497837352	34.0	34.0	34.0	31.2	34.0
15-19	32.6350188479839	34.0	34.0	34.0	31.2	34.0
20-24	32.47546539728596	34.0	34.0	34.0	31.2	34.0
25-29	32.4603312227646	34.0	34.0	34.0	28.4	34.0
30-34	31.9831648578437	34.0	34.0	34.0	22.8	34.0
35-39	31.949530237499367	34.0	34.0	34.0	22.8	34.0
40-44	32.31906853096936	34.0	34.0	34.0	25.6	34.0
45-49	32.289345563895814	34.0	34.0	34.0	28.4	34.0
50-54	32.20795688657352	34.0	34.0	34.0	25.6	34.0
55-59	32.12824786547172	34.0	34.0	34.0	22.8	34.0
60-64	32.06457982710006	34.0	34.0	34.0	22.8	34.0
65-69	31.450792452374014	34.0	34.0	34.0	20.0	34.0
70-74	31.315977674935812	34.0	34.0	34.0	20.0	34.0
75-79	32.05372987226764	34.0	34.0	34.0	20.0	34.0
80-84	31.84436190904132	34.0	34.0	34.0	20.0	34.0
85-89	31.888928579473685	34.0	34.0	34.0	20.0	34.0
90-94	31.83073779611406	34.0	34.0	34.0	22.8	34.0
95-99	31.114349924218754	34.0	34.0	34.0	20.0	34.0
100-104	31.6143700589714	34.0	34.0	34.0	20.0	34.0
105-109	31.01024792655835	34.0	34.0	34.0	20.0	34.0
110-114	31.607028614816972	34.0	34.0	34.0	20.0	34.0
115-119	31.38367766640024	34.0	34.0	34.0	20.0	34.0
120-124	31.205426603359353	34.0	34.0	34.0	20.0	34.0
125-129	31.04915315091062	34.0	34.0	34.0	20.0	34.0
130-134	30.86164577072941	34.0	34.0	34.0	20.0	34.0
135-139	30.877619337880883	34.0	34.0	34.0	20.0	34.0
140-144	31.009282220671782	34.0	34.0	34.0	20.0	34.0
145-149	30.41911709326198	34.0	31.2	34.0	20.0	34.0
150-154	30.546366292709752	34.0	31.2	34.0	20.0	34.0
155-159	30.475085029681555	34.0	34.0	34.0	20.0	34.0
160-164	30.192616348278147	34.0	34.0	34.0	20.0	34.0
165-169	30.399619418058997	34.0	34.0	34.0	20.0	34.0
170-174	29.77555448517695	34.0	28.4	34.0	17.8	34.0
175-179	30.195376073056035	34.0	34.0	34.0	20.0	34.0
180-184	30.304849718249365	34.0	31.2	34.0	20.0	34.0
185-189	29.68129745372163	34.0	22.8	34.0	20.0	34.0
190-194	29.471078779791487	34.0	28.4	34.0	17.8	34.0
195-199	30.067683258647925	34.0	31.2	34.0	17.8	34.0
200-204	28.975306371197284	34.0	25.6	34.0	17.8	34.0
205-209	28.803676948353303	34.0	20.0	34.0	17.8	34.0
210-214	28.521024475196107	34.0	20.0	34.0	15.6	34.0
215-219	28.39482708948329	34.0	20.0	34.0	13.4	34.0
220-224	28.7154203303901	34.0	20.0	34.0	17.8	34.0
225-229	27.980740099249623	34.0	20.0	34.0	15.6	34.0
230-234	28.524014674250402	34.0	20.0	34.0	17.8	34.0
235-239	27.534201684269398	34.0	20.0	34.0	11.2	34.0
240-244	26.273702083208303	31.2	20.0	34.0	9.0	34.0
245-249	26.57104215956351	34.0	20.0	34.0	11.2	34.0
250-254	26.642254494886743	34.0	20.0	34.0	11.2	34.0
255-259	25.771614985130203	31.2	20.0	34.0	11.2	34.0
260-264	26.278263228282526	34.0	20.0	34.0	11.2	34.0
265-269	25.517618429316187	28.4	20.0	34.0	9.0	34.0
270-274	25.625622319463343	25.6	20.0	34.0	9.0	34.0
275-279	24.0505984255306	20.0	20.0	34.0	9.0	34.0
280-284	24.66418965422412	20.0	20.0	34.0	9.0	34.0
285-289	24.736951018201648	20.0	20.0	34.0	9.0	34.0
290-294	23.544250625647635	20.0	20.0	34.0	9.0	34.0
295-299	23.621319310331266	20.0	20.0	34.0	9.0	34.0
300-301	20.940760563571853	20.0	14.5	27.0	9.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-1.3933314455221435
1101	2	-1.1345843964868934
1101	3	-0.18145712765261734
1101	4	-0.4808946798761724
1101	5	-0.13549628814227077
1101	6	-0.6494320886171998
1101	7	-0.05727822127258975
1101	8	-0.5792155811273467
1101	9	-0.12741545672329835
1101	10-14	-0.7202938110662878
1101	15-19	-0.3460299457866043
1101	20-24	-0.6999679616572365
1101	25-29	-0.5009880348372278
1101	30-34	-0.6357023375754522
1101	35-39	-0.6352507075156417
1101	40-44	-0.572331449998341
1101	45-49	-0.28320445005086725
1101	50-54	-0.6643802444820572
1101	55-59	-0.7044322876137521
1101	60-64	-0.5789072812516913
1101	65-69	-0.998459499553018
1101	70-74	-0.9389908367435744
1101	75-79	-0.6968830847612857
1101	80-84	-0.9664278322073052
1101	85-89	-0.9312328899915663
1101	90-94	-0.8216789080555351
1101	95-99	-1.1372485720029708
1101	100-104	-0.8675255571631553
1101	105-109	-1.1879819985395947
1101	110-114	-0.911701998185265
1101	115-119	-1.1792601513425751
1101	120-124	-1.449325983819591
1101	125-129	-1.3422107308114022
1101	130-134	-1.4219181250165107
1101	135-139	-1.416304921559707
1101	140-144	-1.713485192575959
1101	145-149	-1.498303105966766
1101	150-154	-1.2985941390508096
1101	155-159	-1.6074142835268006
1101	160-164	-1.5856526841689558
1101	165-169	-1.5331764725406103
1101	170-174	-1.859676493245793
1101	175-179	-1.833635584631665
1101	180-184	-1.578149223710195
1101	185-189	-2.056533926203162
1101	190-194	-1.7876731777304578
1101	195-199	-1.9829946752278893
1101	200-204	-2.2104984562837764
1101	205-209	-2.4274715993492
1101	210-214	-2.1985292441806514
1101	215-219	-2.4887378009958994
1101	220-224	-2.6785104950985144
1101	225-229	-2.953094500596645
1101	230-234	-3.1233931047806998
1101	235-239	-3.601187046503057
1101	240-244	-3.527694181662916
1101	245-249	-3.760233061448808
1101	250-254	-4.308929108002058
1101	255-259	-3.8311601310402175
1101	260-264	-3.903336361092471
1101	265-269	-4.242943450614074
1101	270-274	-4.018316861051332
1101	275-279	-3.838966269325436
1101	280-284	-4.266807043328495
1101	285-289	-4.569544173317478
1101	290-294	-4.39978423619727
1101	295-299	-4.443562870798878
1101	300-301	-2.502843165754882
1102	1	0.02906314683256639
1102	2	-0.31650970468123774
1102	3	-0.6766917697327983
1102	4	-0.014420526763146313
1102	5	-0.03247132181056145
1102	6	-0.15610099983651082
1102	7	-0.7313691774520166
1102	8	-0.43451963168157803
1102	9	-0.8115551024292955
1102	10-14	-0.3331343787654575
1102	15-19	-0.4991844055400563
1102	20-24	-0.6835740944656905
1102	25-29	-0.4230278152163791
1102	30-34	-0.8107411856019695
1102	35-39	-1.06063659791241
1102	40-44	-0.7401678739080069
1102	45-49	-0.8778330629173325
1102	50-54	-0.605787061015775
1102	55-59	-0.980103063538337
1102	60-64	-0.5711713442372925
1102	65-69	-1.247480529284708
1102	70-74	-0.8843523823770525
1102	75-79	-0.8237244812183491
1102	80-84	-1.1382086257429904
1102	85-89	-0.6961349929647369
1102	90-94	-0.954241323884812
1102	95-99	-1.355239427581573
1102	100-104	-1.2725264010230966
1102	105-109	-1.6110050943371732
1102	110-114	-1.133438532127908
1102	115-119	-1.430720029047798
1102	120-124	-1.2294900821227834
1102	125-129	-1.4414577480910538
1102	130-134	-1.5316311615730172
1102	135-139	-1.7436599774755663
1102	140-144	-1.5205696079061717
1102	145-149	-1.5629632393296795
1102	150-154	-1.6560433268452002
1102	155-159	-1.7828002273229195
1102	160-164	-2.1533406845876044
1102	165-169	-1.975499461708015
1102	170-174	-2.221848768090112
1102	175-179	-1.9262065552133194
1102	180-184	-2.1872334554984434
1102	185-189	-2.4318911170656357
1102	190-194	-2.3081221194824373
1102	195-199	-2.103451506993089
1102	200-204	-2.1263496052567312
1102	205-209	-3.2142532312757943
1102	210-214	-1.7992431424441477
1102	215-219	-2.8202356332894354
1102	220-224	-2.9946258445590708
1102	225-229	-2.798336591998421
1102	230-234	-3.0184598181075373
1102	235-239	-2.991058486381373
1102	240-244	-3.479846256535957
1102	245-249	-3.7504294725831464
1102	250-254	-3.8090042607250467
1102	255-259	-4.1532921775416405
1102	260-264	-4.529554917793501
1102	265-269	-4.346953253752144
1102	270-274	-4.4642569930618805
1102	275-279	-4.297181476021311
1102	280-284	-4.625633818976098
1102	285-289	-5.031725308136931
1102	290-294	-4.842207881655774
1102	295-299	-4.989425904404687
1102	300-301	-3.9176589823278363
1103	1	-0.14602233238530005
1103	2	-1.1133496448457834
1103	3	-0.523844243287666
1103	4	-0.70564020835306
1103	5	-0.7760336682150921
1103	6	-0.8370898875381876
1103	7	-0.7077021190055994
1103	8	-0.5495693164769619
1103	9	-0.20096636431625114
1103	10-14	-0.33233835354908337
1103	15-19	-0.5209495513889735
1103	20-24	-0.5676161929804913
1103	25-29	-0.21103401245967746
1103	30-34	-0.5893446688801021
1103	35-39	-0.27218163477532187
1103	40-44	-0.3894813689395349
1103	45-49	-0.43599632822345313
1103	50-54	-0.4324138373807749
1103	55-59	-0.47138952576720783
1103	60-64	-0.5799676900007285
1103	65-69	-0.5212277244978729
1103	70-74	-0.693154070591607
1103	75-79	-0.33963430064349964
1103	80-84	-0.6125755452856829
1103	85-89	-0.7167034297635446
1103	90-94	-0.5630056149415488
1103	95-99	-0.7540317330309527
1103	100-104	-0.9026599021813553
1103	105-109	-0.8916681813338627
1103	110-114	-0.822162571709594
1103	115-119	-1.1354990223660444
1103	120-124	-1.1246575276577921
1103	125-129	-1.0291801942286227
1103	130-134	-1.5150214816487164
1103	135-139	-1.313107749316373
1103	140-144	-1.353103020199736
1103	145-149	-0.9801007512746587
1103	150-154	-1.5051692048280394
1103	155-159	-1.6410204356958324
1103	160-164	-1.9743745888547295
1103	165-169	-1.8007517819721102
1103	170-174	-2.2583628249670227
1103	175-179	-1.858568631766012
1103	180-184	-1.769528626415429
1103	185-189	-1.8589070345465437
1103	190-194	-2.325733978587561
1103	195-199	-2.0531781491799457
1103	200-204	-2.6798952816469495
1103	205-209	-2.7731059525083808
1103	210-214	-2.3225414321894498
1103	215-219	-2.7720518390121534
1103	220-224	-2.5429263904510826
1103	225-229	-3.3296288928489126
1103	230-234	-3.8120602398759047
1103	235-239	-3.887166322437441
1103	240-244	-3.2223414263360546
1103	245-249	-4.465106082103521
1103	250-254	-4.414165860634945
1103	255-259	-4.084463534210595
1103	260-264	-4.38734360863198
1103	265-269	-4.617391399214679
1103	270-274	-4.408187616368114
1103	275-279	-4.280666597884846
1103	280-284	-4.74105338431367
1103	285-289	-5.117476779242136
1103	290-294	-5.0490395915424315
1103	295-299	-4.408571832522512
1103	300-301	-3.148020245326329
1104	1	-0.7197559460219694
1104	2	-0.7176331975566477
1104	3	-0.34308876331283855
1104	4	-0.2730000455271764
1104	5	-0.5572899449119753
1104	6	-0.6313280471771883
1104	7	-0.4137116796702962
1104	8	-1.1672431558738623
1104	9	-0.07824438683871193
1104	10-14	-0.6373021328235993
1104	15-19	-0.7024361410168538
1104	20-24	-0.5111605537985895
1104	25-29	-0.601782999314942
1104	30-34	-0.7877931571074548
1104	35-39	-1.0199254680996575
1104	40-44	-0.8124748379307327
1104	45-49	-0.9133159158523156
1104	50-54	-0.8249328636667386
1104	55-59	-0.6858468043336217
1104	60-64	-0.9245323793730016
1104	65-69	-1.1934304556470074
1104	70-74	-1.0749320162924754
1104	75-79	-0.8808147400022719
1104	80-84	-0.8499696188000101
1104	85-89	-1.11969905776591
1104	90-94	-0.9342907663422046
1104	95-99	-1.4014382208595286
1104	100-104	-1.6028623094788195
1104	105-109	-1.3498481349151206
1104	110-114	-1.2106658372139911
1104	115-119	-1.8613261935216556
1104	120-124	-1.8742958932863374
1104	125-129	-1.5623426058441083
1104	130-134	-1.9525430804773798
1104	135-139	-1.6980557315126177
1104	140-144	-2.0560644499114495
1104	145-149	-2.1785662083280855
1104	150-154	-1.9819285711162067
1104	155-159	-2.210205502460152
1104	160-164	-2.251092726360266
1104	165-169	-2.307025770450821
1104	170-174	-2.4817135424909686
1104	175-179	-2.4447712894481555
1104	180-184	-2.4830829668118746
1104	185-189	-2.4589343756265123
1104	190-194	-2.747374443385926
1104	195-199	-2.883580433299336
1104	200-204	-3.3048010962328824
1104	205-209	-3.6851648245400384
1104	210-214	-2.888030383376787
1104	215-219	-3.7618564234448755
1104	220-224	-3.622460202918976
1104	225-229	-3.7896605622502726
1104	230-234	-3.669846687351903
1104	235-239	-4.075856499463185
1104	240-244	-4.443705442760095
1104	245-249	-4.539920519167655
1104	250-254	-4.72770945449064
1104	255-259	-5.290635690706186
1104	260-264	-5.250955797300975
1104	265-269	-5.344967109921246
1104	270-274	-5.162390326395215
1104	275-279	-5.011436840315884
1104	280-284	-5.144759556075684
1104	285-289	-5.155744726482659
1104	290-294	-5.474255413740341
1104	295-299	-5.001206291553924
1104	300-301	-3.5780320400189645
1201	1	-0.8004725941654058
1201	2	-0.20497268149500059
1201	3	-0.5186086411934241
1201	4	-0.31297005128499933
1201	5	-0.4540441394192811
1201	6	-0.09625219120311357
1201	7	-0.6501104959689528
1201	8	-1.0390981122884781
1201	9	-1.0726941130073477
1201	10-14	-0.4328619137585079
1201	15-19	-0.8806354152633133
1201	20-24	-0.5581921092108537
1201	25-29	-0.9696810009578485
1201	30-34	-0.936224607317115
1201	35-39	-0.8730020165208003
1201	40-44	-0.7475209726278145
1201	45-49	-1.082453021136839
1201	50-54	-1.0601927603033658
1201	55-59	-0.8107554819860354
1201	60-64	-1.4458071040530314
1201	65-69	-1.274589640645793
1201	70-74	-1.376805886779234
1201	75-79	-1.1943904318091114
1201	80-84	-0.924760720265013
1201	85-89	-1.3883154440925587
1201	90-94	-1.2399717213069401
1201	95-99	-1.9050898757700025
1201	100-104	-1.6780916375933224
1201	105-109	-1.4910758933857586
1201	110-114	-1.4575430531633735
1201	115-119	-1.3792010639738415
1201	120-124	-1.8547109917426745
1201	125-129	-1.744582282929148
1201	130-134	-1.2237364044997676
1201	135-139	-2.0181945490050843
1201	140-144	-1.6431664862516477
1201	145-149	-1.8049330592447177
1201	150-154	-2.036591149916717
1201	155-159	-1.9214928358164514
1201	160-164	-2.5315722468397865
1201	165-169	-1.8887278064925503
1201	170-174	-2.2952310887744716
1201	175-179	-2.4240514773453654
1201	180-184	-2.665978794388554
1201	185-189	-2.278246340257631
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2102	230-234	2.702939357557792
2102	235-239	2.8714553750140404
2102	240-244	2.8053575994608444
2102	245-249	3.0937868261186416
2102	250-254	3.2230195346149593
2102	255-259	3.3830066300337656
2102	260-264	3.5375835437449616
2102	265-269	3.0790730835469198
2102	270-274	3.445327579940873
2102	275-279	3.3311665381295406
2102	280-284	3.6296802110820146
2102	285-289	3.9349596015227064
2102	290-294	3.9454374560506658
2102	295-299	3.7655833538788386
2102	300-301	2.3312911300047574
2103	1	1.0893064749330357
2103	2	1.2178309546594228
2103	3	0.9037634892938868
2103	4	0.5462152660155013
2103	5	1.1661158053479959
2103	6	0.5532789059719647
2103	7	1.11399973934547
2103	8	0.9889497560735272
2103	9	0.6672518049490392
2103	10-14	0.5997541612659418
2103	15-19	0.772244165888516
2103	20-24	0.8005703325358837
2103	25-29	0.7193583700269386
2103	30-34	0.960373414084053
2103	35-39	0.929944249811296
2103	40-44	0.7384428011312423
2103	45-49	0.8997179574908287
2103	50-54	0.7249821697534529
2103	55-59	0.9056112221759491
2103	60-64	0.9597941188710237
2103	65-69	1.3215110851614504
2103	70-74	1.4171613636172076
2103	75-79	1.0563171221998786
2103	80-84	1.1444809790245323
2103	85-89	1.154042102666903
2103	90-94	1.099397569689149
2103	95-99	1.405693073338309
2103	100-104	1.1991271256408886
2103	105-109	1.5598022584278652
2103	110-114	1.1571055978489149
2103	115-119	1.3860901007648323
2103	120-124	1.463184883611767
2103	125-129	1.5075326575835817
2103	130-134	1.6941740868913868
2103	135-139	1.6777747822852618
2103	140-144	1.6043121543685395
2103	145-149	1.91313924756831
2103	150-154	1.8595150681174886
2103	155-159	1.9518896399664776
2103	160-164	2.114709096126635
2103	165-169	1.9886088711506709
2103	170-174	2.0725836520272196
2103	175-179	2.011516290375333
2103	180-184	2.02247777079306
2103	185-189	2.0031865973231824
2103	190-194	2.096016530281293
2103	195-199	2.148717750790972
2103	200-204	2.3808176273990327
2103	205-209	2.39163140122173
2103	210-214	2.851785232966705
2103	215-219	2.4956098161673133
2103	220-224	2.321578543286922
2103	225-229	2.3485196522305714
2103	230-234	2.7803440277936353
2103	235-239	2.9058333239349317
2103	240-244	2.721308407655414
2103	245-249	3.137004688588579
2103	250-254	3.3362809269622318
2103	255-259	3.803677233668356
2103	260-264	3.6768761071372467
2103	265-269	3.881604456312491
2103	270-274	3.891446792718355
2103	275-279	3.7318881881399975
2103	280-284	4.218056515563294
2103	285-289	4.052920788983272
2103	290-294	3.7437184391375133
2103	295-299	3.7779873613273978
2103	300-301	4.525598776703774
2104	1	0.6970075973400398
2104	2	0.7981770791231959
2104	3	0.7802546451968126
2104	4	0.32011783403571314
2104	5	0.4416637867783315
2104	6	0.5908295905814711
2104	7	0.9308593503227307
2104	8	0.7929986483577132
2104	9	0.6885540321336237
2104	10-14	0.5381686879157641
2104	15-19	0.5428324011826362
2104	20-24	0.7622351383183315
2104	25-29	0.6787019676058392
2104	30-34	0.9221792553016641
2104	35-39	0.9082982487535212
2104	40-44	0.7022172619645204
2104	45-49	0.8875330835412498
2104	50-54	0.8670817355401326
2104	55-59	0.8366912681353469
2104	60-64	1.059808849448764
2104	65-69	1.3697159471610938
2104	70-74	1.2808265310113534
2104	75-79	1.0002236503130817
2104	80-84	1.1042162798760877
2104	85-89	1.2048607940900276
2104	90-94	1.1712784550723505
2104	95-99	1.4609564061546472
2104	100-104	1.2695057842720452
2104	105-109	1.3374490652728213
2104	110-114	1.3218291187020945
2104	115-119	1.2887138827614777
2104	120-124	1.4994430857358054
2104	125-129	1.6137166517772954
2104	130-134	1.734275993190927
2104	135-139	1.7362079729300248
2104	140-144	1.8073337946452277
2104	145-149	1.67091478709402
2104	150-154	1.6247425057961635
2104	155-159	1.9208052562181628
2104	160-164	2.234400118596387
2104	165-169	1.89959934705902
2104	170-174	2.226302195122628
2104	175-179	2.2522462238696264
2104	180-184	2.0945083426829214
2104	185-189	2.391427380579742
2104	190-194	2.234009213171994
2104	195-199	2.0879901872106217
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2104	210-214	3.288990745439264
2104	215-219	3.072031345372327
2104	220-224	2.6629257568216076
2104	225-229	2.5626009080015812
2104	230-234	2.8665829698006036
2104	235-239	2.7464701451901057
2104	240-244	2.780029701552298
2104	245-249	3.332573085507452
2104	250-254	3.5281304614240057
2104	255-259	3.405617038493226
2104	260-264	2.9949130067348015
2104	265-269	3.1544016429235064
2104	270-274	3.5644754417988587
2104	275-279	3.2291055114880898
2104	280-284	3.438117911190723
2104	285-289	3.5138162082746547
2104	290-294	3.382403099835564
2104	295-299	3.5506211871721405
2104	300-301	3.695029626303196
2201	1	1.2140675833897951
2201	2	0.9385368791696962
2201	3	0.7106196765848622
2201	4	0.5358234838845917
2201	5	1.0411755282338042
2201	6	0.7093049451501798
2201	7	0.8362883203297038
2201	8	1.1201609361977454
2201	9	0.222107275565385
2201	10-14	0.6491748492735994
2201	15-19	0.6238746006711295
2201	20-24	0.6161818948865658
2201	25-29	0.655221962955757
2201	30-34	0.8251727451877358
2201	35-39	0.866470766772597
2201	40-44	0.6915424189047741
2201	45-49	0.6531341065591576
2201	50-54	0.6562471004749071
2201	55-59	0.17119777659649316
2201	60-64	-0.6472170517765079
2201	65-69	0.16259850183057978
2201	70-74	0.23227556563211849
2201	75-79	0.15106232111065054
2201	80-84	0.06456735496629307
2201	85-89	-0.13001724903719492
2201	90-94	0.04066986825775132
2201	95-99	0.47364224232982366
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2201	140-144	0.9341529083496916
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2201	155-159	1.460680670190726
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2202	1	1.3024233039035202
2202	2	0.1840226220706036
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2202	4	0.23651277338618115
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2202	8	0.3611913356321814
2202	9	0.7159876219775398
2202	10-14	0.5904953983486436
2202	15-19	0.6226506699535577
2202	20-24	0.6884600850597806
2202	25-29	0.7253129845181476
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2202	80-84	1.223474909459707
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2203	8	0.6222062844913445
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2204	1	1.0923284529550124
2204	2	1.0394426762711433
2204	3	0.590088275618676
2204	4	0.11517130997154368
2204	5	1.141054755286941
2204	6	0.5965633992564605
2204	7	0.7395491898949196
2204	8	1.0076005497243194
2204	9	0.7750662127634484
2204	10-14	0.6197907913025773
2204	15-19	0.5694663881107473
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2204	55-59	0.6565662560000689
2204	60-64	0.7020635486056364
2204	65-69	1.1631442220085724
2204	70-74	1.1159389597865434
2204	75-79	0.9186267211010204
2204	80-84	1.0758667537124147
2204	85-89	0.9391614654515017
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2204	295-299	3.939453091642438
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2301	1	-2.7439794489523415
2301	2	1.342939497342897
2301	3	0.5630068345045345
2301	4	0.5043402473012435
2301	5	0.7792397386354146
2301	6	0.9192548918389605
2301	7	0.8574647212300697
2301	8	0.33013309589446393
2301	9	0.28684338503108364
2301	10-14	0.5174383843997603
2301	15-19	0.5297373538994066
2301	20-24	0.58710654836365
2301	25-29	0.7046399821477962
2301	30-34	0.8536557448323308
2301	35-39	0.8071413034388648
2301	40-44	0.7462234401107821
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2301	50-54	0.8292849514404352
2301	55-59	0.777914341027163
2301	60-64	0.9388216666656959
2301	65-69	1.2187711642272596
2301	70-74	1.2628611184078729
2301	75-79	0.9024921592840016
2301	80-84	0.9026077007876161
2301	85-89	1.0274913806616404
2301	90-94	0.879235913163857
2301	95-99	1.2184439588164686
2301	100-104	1.145789554121503
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2301	110-114	1.2218423762220603
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2301	120-124	1.544515707484024
2301	125-129	1.4884051000582303
2301	130-134	1.6480991673358147
2301	135-139	1.6030215679790558
2301	140-144	1.8219247443568243
2301	145-149	1.8576547187035573
2301	150-154	1.8848168047200815
2301	155-159	1.961207327134499
2301	160-164	1.9771796073666117
2301	165-169	1.9802941823236786
2301	170-174	2.3241581615625506
2301	175-179	2.348554172968214
2301	180-184	2.180120496391595
2301	185-189	2.263395415794186
2301	190-194	2.377426815960792
2301	195-199	2.1704930121945694
2301	200-204	2.351628047984274
2301	205-209	3.0433141119240474
2301	210-214	-2.6233636016088333
2301	215-219	2.361537794006651
2301	220-224	2.8196394851151823
2301	225-229	2.98840884890647
2301	230-234	3.0329756067410045
2301	235-239	3.213866564932161
2301	240-244	3.1213084076554196
2301	245-249	3.813115799699691
2301	250-254	3.7811208777163046
2301	255-259	3.6667551037822363
2301	260-264	3.832156193317605
2301	265-269	3.8990467462478513
2301	270-274	3.957270521639451
2301	275-279	3.780707580224707
2301	280-284	3.9169174714912884
2301	285-289	4.440166552354519
2301	290-294	4.195466777943324
2301	295-299	3.864561855739783
2301	300-301	3.3693572220925674
2302	1	1.4460547402178854
2302	2	0.8262990448348297
2302	3	1.2043159178951015
2302	4	0.40995450780352627
2302	5	0.7298758934983276
2302	6	0.8121460550830193
2302	7	1.045579000193026
2302	8	1.0928986527053475
2302	9	0.6401068631699545
2302	10-14	0.6333138265329339
2302	15-19	0.8443308867557207
2302	20-24	0.9133923343822659
2302	25-29	0.796857699965301
2302	30-34	1.0949471059748177
2302	35-39	0.9563729701424499
2302	40-44	0.9059924601443043
2302	45-49	0.9783668154079876
2302	50-54	1.0201904844739644
2302	55-59	1.0221617448240323
2302	60-64	1.1211092265275937
2302	65-69	1.2576298075329397
2302	70-74	1.467048259180384
2302	75-79	1.036388344399704
2302	80-84	1.269003364744254
2302	85-89	0.9947283442400625
2302	90-94	1.2543215321519092
2302	95-99	1.5478283955179784
2302	100-104	1.4216494665667803
2302	105-109	1.6294969884330186
2302	110-114	1.4074717874612226
2302	115-119	1.566159627728311
2302	120-124	1.8136333107479743
2302	125-129	1.6114120389188429
2302	130-134	1.7508151505396015
2302	135-139	1.6728398379081924
2302	140-144	1.9619099623908163
2302	145-149	1.927810825223304
2302	150-154	2.1893029074323493
2302	155-159	2.1206599464540794
2302	160-164	2.3145399065894416
2302	165-169	2.124306366247172
2302	170-174	2.31879185996565
2302	175-179	2.300983118745272
2302	180-184	2.2419091142777745
2302	185-189	2.616711279531337
2302	190-194	2.299440746843903
2302	195-199	2.4385034939522683
2302	200-204	2.7968206856099336
2302	205-209	3.13979706570386
2302	210-214	3.3617282548345955
2302	215-219	2.7926362933572904
2302	220-224	2.9137562489974975
2302	225-229	3.051625298245476
2302	230-234	3.020661123485361
2302	235-239	3.239791239691371
2302	240-244	3.4293041136512628
2302	245-249	3.520879651991951
2302	250-254	3.933736053868067
2302	255-259	3.7048580848120416
2302	260-264	4.108294404699723
2302	265-269	4.209426150041896
2302	270-274	4.591270378211831
2302	275-279	4.4827918057634335
2302	280-284	4.594228013147831
2302	285-289	4.90969837164041
2302	290-294	4.935482854039048
2302	295-299	4.668600331059341
2302	300-301	4.987306935509974
2303	1	0.7189677972173101
2303	2	0.32878693856622476
2303	3	0.1987403703363455
2303	4	0.11476147390597191
2303	5	-0.15079406074220003
2303	6	0.7587674065424324
2303	7	0.5017076598402745
2303	8	0.3090122072835193
2303	9	0.5404578338927735
2303	10-14	0.3614847803736154
2303	15-19	0.5039381549595632
2303	20-24	0.5272712272695657
2303	25-29	0.24831711619880537
2303	30-34	0.558797121524151
2303	35-39	0.419618782928449
2303	40-44	0.3192360515966257
2303	45-49	0.42247361713174314
2303	50-54	0.5109186376571344
2303	55-59	0.5641982635498763
2303	60-64	0.7469255666582129
2303	65-69	0.4632142404264563
2303	70-74	0.8638939653423598
2303	75-79	0.5459978339372284
2303	80-84	0.5884000505143909
2303	85-89	0.8873935003998277
2303	90-94	0.7594616983076783
2303	95-99	0.9519334201747789
2303	100-104	1.027420491490144
2303	105-109	0.9203450936353761
2303	110-114	0.9699268054540262
2303	115-119	1.1331417237787846
2303	120-124	1.0834276152483895
2303	125-129	1.1809619967134992
2303	130-134	1.2506152305076057
2303	135-139	1.3477654232296175
2303	140-144	1.365441882955924
2303	145-149	1.5006705702141971
2303	150-154	1.2544279685190247
2303	155-159	1.5664505191139035
2303	160-164	1.406258774597788
2303	165-169	1.4742019635637966
2303	170-174	1.889212580566305
2303	175-179	1.821294993995167
2303	180-184	1.8358582013096267
2303	185-189	1.8305724017491158
2303	190-194	1.9315567893158025
2303	195-199	1.8039573124248882
2303	200-204	1.9260134529474406
2303	205-209	2.535841168610645
2303	210-214	2.6955783494999253
2303	215-219	2.4673944327195088
2303	220-224	2.1258437123378897
2303	225-229	2.4639251089148146
2303	230-234	2.2596276008213714
2303	235-239	2.362039448474828
2303	240-244	2.666856922056052
2303	245-249	3.131045594747267
2303	250-254	2.9665013952201136
2303	255-259	2.540027554314957
2303	260-264	3.3215952854279323
2303	265-269	3.20732513452624
2303	270-274	3.082897585692699
2303	275-279	3.0091490528523863
2303	280-284	3.3730443055510833
2303	285-289	3.1365199196576157
2303	290-294	3.706824135669393
2303	295-299	3.6253763233403227
2303	300-301	3.4130633822425764
2304	1	0.9134822991088605
2304	2	1.2782705150989848
2304	3	0.42299425852466044
2304	4	0.491270211070443
2304	5	0.6468850361172223
2304	6	0.4543778070708697
2304	7	1.0370766624223968
2304	8	0.6812574483812206
2304	9	0.8293397170369516
2304	10-14	0.5519519634637433
2304	15-19	0.7041122977566516
2304	20-24	0.7263945083600589
2304	25-29	0.7929847436533208
2304	30-34	1.0372964910071332
2304	35-39	0.9178563377233822
2304	40-44	0.752179064867498
2304	45-49	0.9107069684798503
2304	50-54	0.7804766752479537
2304	55-59	0.9957211122858425
2304	60-64	1.0477062067831113
2304	65-69	1.2019700201658416
2304	70-74	1.3320918876386436
2304	75-79	1.0722723790642235
2304	80-84	1.052058250238165
2304	85-89	1.0879309411012024
2304	90-94	1.1435216274495694
2304	95-99	1.5222901126626134
2304	100-104	1.2479873563193102
2304	105-109	1.721852329568847
2304	110-114	1.4498643542440313
2304	115-119	1.6097285249557487
2304	120-124	1.6648453778136982
2304	125-129	1.604888616541757
2304	130-134	1.929417848654495
2304	135-139	1.8016986484743214
2304	140-144	1.6586713311034096
2304	145-149	2.096980544393027
2304	150-154	1.975364136176502
2304	155-159	2.1666276643730136
2304	160-164	2.008537643270106
2304	165-169	2.0220251786001278
2304	170-174	2.249605441555282
2304	175-179	2.3816120242078895
2304	180-184	2.2693766947387033
2304	185-189	2.438051063177106
2304	190-194	2.490925438550317
2304	195-199	2.196388328998971
2304	200-204	2.5857162935742757
2304	205-209	2.914546780143155
2304	210-214	3.2556206152457996
2304	215-219	2.886658555893966
2304	220-224	3.172207726074255
2304	225-229	2.7701766655773383
2304	230-234	2.6218571189163953
2304	235-239	3.3665981110658727
2304	240-244	3.2167976745372826
2304	245-249	3.8656891662027952
2304	250-254	3.8291603634830658
2304	255-259	3.7099690615416527
2304	260-264	3.832243429313934
2304	265-269	4.1605068013442725
2304	270-274	3.865162290684676
2304	275-279	4.137758322370743
2304	280-284	4.224700225388819
2304	285-289	4.174339842911984
2304	290-294	4.838807621796526
2304	295-299	4.747112562186025
2304	300-301	4.539954414359812
2401	1	0.5882179408256327
2401	2	1.47964932496836
2401	3	0.7713251728933059
2401	4	0.5349152452815176
2401	5	0.9236318741848173
2401	6	0.9568658850306448
2401	7	1.018727087469049
2401	8	0.8447449043215052
2401	9	1.0057340773935763
2401	10-14	0.7754331992091323
2401	15-19	0.8089018478292189
2401	20-24	0.9499068450799442
2401	25-29	0.9182698359195385
2401	30-34	1.329863358102294
2401	35-39	1.136340683566214
2401	40-44	1.0365456416869137
2401	45-49	1.1340845343102401
2401	50-54	1.1481212947793722
2401	55-59	1.172592354251421
2401	60-64	1.3765471771335243
2401	65-69	1.5716569370225706
2401	70-74	1.4653491963676508
2401	75-79	1.3324942326823077
2401	80-84	1.3667586441838502
2401	85-89	1.3963178372239433
2401	90-94	1.497481818202214
2401	95-99	2.0691178132638974
2401	100-104	1.7869465103727968
2401	105-109	2.0220389354158286
2401	110-114	1.8129371305243573
2401	115-119	2.009438249193771
2401	120-124	2.1481441545087065
2401	125-129	1.8330557324447199
2401	130-134	2.162986167128466
2401	135-139	2.0537513127911353
2401	140-144	2.2224801637801654
2401	145-149	2.4202337063254333
2401	150-154	2.5157435680653677
2401	155-159	2.3622424269689013
2401	160-164	2.616841603460852
2401	165-169	2.5094603849032673
2401	170-174	2.676610397719763
2401	175-179	2.7913081028576485
2401	180-184	2.6510998315218686
2401	185-189	2.9323030661769636
2401	190-194	3.047950231938753
2401	195-199	2.73346109042852
2401	200-204	3.344018254620668
2401	205-209	3.4937645892065845
2401	210-214	3.3438639899385763
2401	215-219	3.504109715369136
2401	220-224	3.694333170669676
2401	225-229	3.6372190571480303
2401	230-234	3.669147964148923
2401	235-239	3.986394209764196
2401	240-244	4.2013974087082
2401	245-249	4.239868103331993
2401	250-254	4.80252399242131
2401	255-259	4.774889275689997
2401	260-264	4.872541039079813
2401	265-269	5.0756954434625925
2401	270-274	4.894394579265164
2401	275-279	4.730470704174039
2401	280-284	5.204648107154885
2401	285-289	5.464342907480596
2401	290-294	5.3983536871989415
2401	295-299	5.95817406613806
2401	300-301	5.292971803060087
2402	1	0.3580893225202324
2402	2	1.0716890530827392
2402	3	0.550293589628339
2402	4	0.21788870127589632
2402	5	0.897153789103367
2402	6	0.6957179886284379
2402	7	0.4966144072862271
2402	8	0.9082044144586199
2402	9	0.4476507538262524
2402	10-14	0.6692835449257686
2402	15-19	0.7037659050189546
2402	20-24	0.7216166774952697
2402	25-29	0.593808919477496
2402	30-34	0.8175640495355552
2402	35-39	0.5920142450334644
2402	40-44	0.6230641580351985
2402	45-49	0.5911775848200236
2402	50-54	0.7698340569966433
2402	55-59	0.6900832718653902
2402	60-64	0.819522976969445
2402	65-69	0.9596623278581511
2402	70-74	1.091002542201469
2402	75-79	0.8015306208224686
2402	80-84	0.9053250920919673
2402	85-89	0.9417532057930238
2402	90-94	1.0562067683955796
2402	95-99	1.3644066917357272
2402	100-104	1.2489250056265142
2402	105-109	1.390593371135978
2402	110-114	1.0017277082812974
2402	115-119	1.2003221704406997
2402	120-124	1.464851350102272
2402	125-129	1.2659911010138103
2402	130-134	1.370495249514498
2402	135-139	1.6096358199984522
2402	140-144	1.4949649288100382
2402	145-149	1.6119291827112292
2402	150-154	1.6424150200752123
2402	155-159	1.745623125433692
2402	160-164	1.9817220675414795
2402	165-169	1.7154855226039523
2402	170-174	2.002282787266232
2402	175-179	1.857354628112546
2402	180-184	1.9625480326234666
2402	185-189	2.0475746588173003
2402	190-194	2.367841240113137
2402	195-199	2.140331100784202
2402	200-204	2.7254029796072636
2402	205-209	2.624478397530801
2402	210-214	3.2838744190755342
2402	215-219	2.871228556119153
2402	220-224	2.50668802572671
2402	225-229	2.6482049043685194
2402	230-234	3.0092479957301137
2402	235-239	3.3281026786640737
2402	240-244	3.793754760882525
2402	245-249	3.924845004996982
2402	250-254	4.112154786284275
2402	255-259	4.03678840684006
2402	260-264	3.9455273710348386
2402	265-269	4.466685256692909
2402	270-274	4.05377970085274
2402	275-279	4.380202856480899
2402	280-284	4.636699502812839
2402	285-289	4.6688005157738175
2402	290-294	4.55425150282279
2402	295-299	4.765460622602699
2402	300-301	0.715915076003359
2404	1	0.5576599446677193
2404	2	1.4896268383337592
2404	3	0.5392288526598037
2404	4	0.603983464667678
2404	5	0.9699528522789613
2404	6	0.6714866650637035
2404	7	1.1336099633755872
2404	8	0.966455675568394
2404	9	0.4860545491661483
2404	10-14	0.5406563529968693
2404	15-19	0.7390949946178011
2404	20-24	0.8045136268106674
2404	25-29	0.6337260465493202
2404	30-34	0.8580997209263828
2404	35-39	0.7392788378751654
2404	40-44	0.6175302882297657
2404	45-49	0.6409232169319665
2404	50-54	0.7674222781215434
2404	55-59	0.8292558600865121
2404	60-64	0.9242396456434179
2404	65-69	1.2875016630382028
2404	70-74	1.272924738997812
2404	75-79	0.936052781487291
2404	80-84	1.1790619944164256
2404	85-89	1.2796069935805754
2404	90-94	1.3090310487761663
2404	95-99	1.5256857474987022
2404	100-104	1.303364665990241
2404	105-109	1.6241444972349797
2404	110-114	1.1284951417390836
2404	115-119	1.2824325522304107
2404	120-124	1.655699537504411
2404	125-129	1.4354584622085511
2404	130-134	1.6093697527061153
2404	135-139	1.6310874037633525
2404	140-144	1.6453277494772784
2404	145-149	1.8863826934904608
2404	150-154	1.908311382835663
2404	155-159	2.1702211622878416
2404	160-164	2.1577096623867185
2404	165-169	2.190875441910226
2404	170-174	2.2523023585055384
2404	175-179	2.211669333640664
2404	180-184	2.2013578001773695
2404	185-189	2.347076344954843
2404	190-194	2.370837274105572
2404	195-199	2.2087117659514277
2404	200-204	3.0273179265154972
2404	205-209	2.8868450988582843
2404	210-214	3.857934971776416
2404	215-219	3.4300090013462103
2404	220-224	3.3305726079611055
2404	225-229	3.2747983009252266
2404	230-234	3.3339644799826225
2404	235-239	3.7988879982567205
2404	240-244	3.996744424925879
2404	245-249	4.587650783390419
2404	250-254	4.351440554935422
2404	255-259	4.487822763620844
2404	260-264	4.826324411815438
2404	265-269	4.701821420363313
2404	270-274	4.928630902655065
2404	275-279	4.725101536123301
2404	280-284	4.835911884899112
2404	285-289	5.128434708779103
2404	290-294	5.196584096379077
2404	295-299	5.287865776517183
2404	300-301	3.255405753864821
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	12.0
16	406.0
17	1016.0
18	1125.0
19	1008.0
20	1101.0
21	1245.0
22	1589.0
23	2084.0
24	2810.0
25	3908.0
26	5629.0
27	8046.0
28	10374.0
29	11925.0
30	11814.0
31	12318.0
32	21875.0
33	18454.0
34	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	99.50922872682112	0.1370390989679243	0.06081110016701641	0.29292107404393813
2	0.5276005310265085	98.38465162091559	0.12247869470258232	0.9652691533553167
3	0.5550083508200934	0.36657958973919746	0.16787289623570725	98.910539163205
4	0.1892852554494454	0.27921716414714576	99.22059012461993	0.3109074557834782
5	0.256948310564858	98.83859363624684	0.5652862832426876	0.3391717699456126
6	0.3126204445205773	0.5978330692475697	0.13875208770502334	98.95079439852682
7	0.45222902659415015	31.811913836666523	37.38940516466104	30.34645197207828
8	34.395957346580445	32.98188514410518	0.5061881718127703	32.1159693375016
9	99.26769731489016	0.15245599760181577	0.24752687251081323	0.3323198149972164
10-14	39.94792514239219	19.936276818979913	39.89482249154212	0.22097554708577793
15-19	0.25182789362241575	39.90375720364825	39.72285226782154	20.1215626349078
20-24	19.41100981250525	0.8899275755898726	39.363789165637215	40.335273446267664
25-29	20.108400433875907	0.3629341115225583	44.25277212775692	35.27589332684462
30-34	0.4083864024294107	34.638124747222015	0.9077728802637802	64.0457159700848
35-39	19.41220731629979	1.476251934656184	54.91389815867008	24.19764259037394
40-44	37.82606906766876	18.116719697177498	22.16103903228461	21.89617220286913
45-49	1.148179648275673	20.206315673955388	20.356868501193446	58.288636176575494
50-54	38.634551238627694	1.5880501796417454	20.732972620711536	39.04442596101902
55-59	36.1842026263198	23.186063865373235	39.41606841125664	1.213665097050322
60-64	21.8984268489933	32.51058554459322	5.385148987462182	40.2058386189513
65-69	35.710699091700114	39.534087899767165	3.2227277886113432	21.53248521992138
70-74	41.74850674481555	17.889980089930873	2.6578307489249076	37.70368241632867
75-79	0.7201389331134821	0.2977629909862151	40.13173860884442	58.85035946705588
80-84	20.365359058577262	19.526155604674063	1.4732109043084274	58.635274432440255
85-89	59.03709047334558	0.9040619947901513	38.80597014925373	1.2528773826105373
90-94	0.41429828443316385	0.33267842291141836	75.43793188357633	23.815091409079088
95-99	2.514985531211244	38.13645445776492	19.030763400854347	40.317796610169495
100-104	0.2897416628172335	18.583282199072	58.97509704018808	22.151879097922684
105-109	20.279745598049743	39.37661522653256	0.7153115286012548	39.628327646816444
110-114	0.3091592523622319	20.162252569142698	43.98268846127319	35.54589971722188
115-119	19.89372673871127	0.7088333396570695	19.98634072980515	59.41109919182651
120-124	0.5889395293313966	54.03684063441242	3.4247851423011766	41.949434693955
125-129	55.08158592619049	28.126223961433677	15.821130383920575	0.9710597284552593
130-134	2.617681777704144	17.416523425904835	40.01404794941358	39.95174684697745
135-139	1.9173796846837514	3.908132894128046	37.5285725137597	56.645914907428505
140-144	0.9793153944719019	1.5890778098978031	53.70623646555386	43.72537033007643
145-149	19.522155293483575	21.930553420246643	18.903385956941808	39.64390532932797
150-154	0.6926670272867258	36.70841448724839	22.1083139053597	40.49060458010518
155-159	21.8779389798628	64.2547438592609	8.24245546581102	5.62486169506528
160-164	21.245013323741855	36.879702433549895	21.23567546442881	20.63960877827945
165-169	16.521734618697273	7.040964922293592	42.04672415522603	34.39057630378311
170-174	22.907710007525754	36.471025838429796	18.951065283772913	21.67019887027153
175-179	17.976713357630633	4.1776125653725416	39.78111663741991	38.06455743957691
180-184	42.33283135137007	4.018279067352132	35.88045905385055	17.768430527427256
185-189	23.555487830218738	36.03730588610137	19.813297408120576	20.59390887555931
190-194	51.43784135848769	19.091477578821006	4.5249433126215814	24.94573775006972
195-199	18.054982865498495	3.8477264300985103	60.94442461685592	17.152866087547082
200-204	2.0401830062186748	38.329539485203995	18.729011756017137	40.90126575256019
205-209	18.54839829002887	38.54721852098601	3.344298245614035	39.56008494337109
210-214	3.620815975478971	21.220176050231302	69.44020698467082	5.718800989618912
215-219	4.1373875010221255	8.10740749117493	31.374797097339346	56.3804079104636
220-224	34.27836263011199	4.960925055143635	20.850648231486623	39.910064083257744
225-229	21.378306786100172	4.303614373845456	17.895681572618592	56.422397267435784
230-234	16.029276357092034	1.3769723349935128	59.17388635444133	23.419864953473123
235-239	18.293804617782424	21.450749530071086	0.5241212722027231	59.73132457994377
240-244	4.574908690561488	52.92889222864932	21.334777973891615	21.161421106897578
245-249	1.6007066828760594	36.00164426213737	22.90597094553819	39.49167810944839
250-254	36.80274419712273	41.13266421057055	4.160596467143877	17.903995125162844
255-259	20.520416122558085	19.630502800150744	20.358279068544537	39.49080200874664
260-264	20.56062302365819	18.30430311687309	19.786230447157752	41.34884341231096
265-269	1.5541473804076211	19.310158191256427	20.58612654597598	58.549567882359966
270-274	18.60649665771049	19.900127216389272	59.3125740116096	2.180802114290641
275-279	21.650543164699677	19.08725040624873	1.1207348294108201	58.141471599640774
280-284	58.39770388133183	0.5991412544475805	19.78563192779181	21.21752293642878
285-289	41.299898824700946	1.7346868261255528	19.835674304131885	37.129740045041615
290-294	21.367103374810824	37.73930177676845	18.732449721062824	22.161145127357894
295-299	37.01568113538909	19.357242830271097	40.76827757125155	2.8587984630882657
300-301	10.509340598714978	1.9300791892648894	0.5593325354993677	87.00124767652076
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.25
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.25
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.25
15	1.0
16	1.0
17	2.0
18	2.8333333333333335
19	2.5
20	2.5
21	1.75
22	2.25
23	2.5
24	3.0
25	3.5
26	3.5
27	2.75
28	1.75
29	3.0
30	3.5
31	3.666666666666667
32	5.999999999999999
33	6.75
34	10.666666666666666
35	11.750000000000002
36	8.166666666666666
37	7.916666666666666
38	9.416666666666666
39	13.833333333333334
40	17.66666666666667
41	19.250000000000004
42	21.166666666666668
43	34.083333333333336
44	55.666666666666686
45	91.16666666666663
46	124.91666666666656
47	734.3333333333334
48	1009.0833333333335
49	417.9166666666665
50	479.41666666666623
51	1156.6666666666717
52	7095.750000000017
53	18749.749999999964
54	5864.5000000000055
55	2381.833333333332
56	1049.0833333333342
57	589.4999999999997
58	146.3333333333333
59	43.75
60	19.583333333333336
61	9.416666666666668
62	7.916666666666667
63	11.750000000000002
64	5.0
65	3.083333333333333
66	1.0
67	0.6666666666666666
68	0.3333333333333333
69	0.0
70	0.0
71	0.75
72	1.0
73	0.5
74	0.3333333333333333
75	0.25
76	0.3333333333333333
77	0.6666666666666666
78	0.3333333333333333
79	0.0
80	0.0
81	0.0
82	0.25
83	0.25
84	0.0
85	0.0
86	0.0
87	0.5
88	1.0
89	1.0
90	1.75
91	2.25
92	1.5
93	1.5833333333333333
94	2.1666666666666665
95	1.5833333333333333
96	4.25
97	7.583333333333333
98	11.166666666666664
99	20.75
100	58.33333333333335
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	1.7264328961429763E-4
140-144	0.0
145-149	0.0
150-154	1.7282081592163612E-4
155-159	0.0
160-164	0.0
165-169	1.7294963879467938E-4
170-174	0.0
175-179	0.0
180-184	0.0
185-189	1.7316107269821316E-4
190-194	0.0
195-199	0.0
200-204	0.0
205-209	1.7349514647327743E-4
210-214	1.8205384818536896
215-219	1.7397839884199977E-4
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
10-19	20.0
20-29	28.0
30-39	29.0
40-49	54.0
50-59	141.0
60-69	250.0
70-79	43.0
80-89	33.0
90-99	103.0
100-109	57.0
110-119	50.0
120-129	43.0
130-139	76.0
140-149	84.0
150-159	80.0
160-169	45.0
170-179	73.0
180-189	77.0
190-199	72.0
200-209	185.0
210-219	298.0
220-229	203.0
230-239	209.0
240-249	231.0
250-259	233.0
260-269	312.0
270-279	509.0
280-289	608.0
290-299	689.0
300-302	111920.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.047150014988652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.80232888383982	6.857950408976062
2	8.198428476758497	1.4834482463277805
3	2.9347723184701318	0.79653976275106
4	2.1679447126763227	0.7845488415913666
5	1.2969800246142196	0.5866986424564258
6	1.1076398750355012	0.6012590467217678
7	0.9277667329357191	0.5875551368249754
8	0.6910915459623213	0.5001927112329236
9	0.6532235160465777	0.5318830028692562
>10	4.8944428666098645	8.656588582930068
>50	0.5396194262993468	3.2992163076527774
>100	0.6532235160465777	12.873110359299389
>500	0.04733503739467954	2.217463920174725
>1k	0.0	0.0
>5k	0.08520306731042318	60.22354503019143
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	9115	7.806946169328937	No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	8995	7.704166845102993	No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	8562	7.333304783521048	No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7569	6.482805875551369	No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7559	6.4742409318658725	No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7365	6.308081024367265	No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7337	6.284099182047878	No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7146	6.120508757654918	No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6666	5.709391460751146	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	528	0.45222902659415015	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	526	0.45051603785705113	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	517	0.4428075885401054	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	512	0.4385251166973577	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	506	0.4333861504860605	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	491	0.4205387349578177	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	466	0.3991263757440795	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	459	0.3931309151642328	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	458	0.3922744207956833	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	437	0.3742880390561432	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	437	0.3742880390561432	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	428	0.36657958973919746	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	418	0.3580146460537022	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	411	0.35201918547385547	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	409	0.35030619673675645	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	380	0.32546786004882017	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	374	0.32032889383752305	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	360	0.30833797267782964	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	287	0.24581388377371416	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	265	0.22697100766562459	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	264	0.22611451329707508	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	261	0.2235450301914265	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	243	0.208128131557535	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	235	0.2012761766091388	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	234	0.20041968224058926	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	229	0.19613721039784165	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	228	0.19528071602929212	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	220	0.18842876108089587	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	215	0.18414628923814827	No Hit
GACTACTAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	205	0.17558134555265298	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	204	0.17472485118410347	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	201	0.17215536807845488	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	198	0.1695858849728063	No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	198	0.1695858849728063	No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	197	0.1687293906042568	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	197	0.1687293906042568	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	195	0.16701640186715774	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	193	0.16530341313005867	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	190	0.16273393002441008	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	189	0.16187743565586057	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	189	0.16187743565586057	No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	186	0.15930795255021196	No Hit
GACTACCAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	185	0.15845145818166245	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	180	0.15416898633891482	No Hit
GACTACACGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	176	0.15074300886471673	No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	175	0.1498865144961672	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	169	0.14474754828487002	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	167	0.14303455954777097	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	156	0.13361312149372617	No Hit
GACTACCGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	155	0.13275662712517666	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	152	0.13018714401952805	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	151	0.12933064965097854	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	148	0.12676116654532996	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	138	0.11819622285983471	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	136	0.11648323412273565	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	134	0.11477024538563659	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	133	0.11391375101708705	No Hit
GACTACCCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	124	0.10620530170014132	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	123	0.1053488073315918	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	122	0.10449231296304226	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	121	0.10363581859449275	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	119	0.10192282985739369	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	117	0.10020984112029463	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	6.851954948396214E-4	0.0	0.0	0.0
175-179	0.0	8.564943685495268E-4	0.0	0.0	0.0
180-184	0.0	8.564943685495268E-4	0.0	0.0	0.0
185-189	0.0	8.564943685495268E-4	0.0	0.0	0.0
190-194	0.0	8.564943685495268E-4	0.0	0.0	0.0
195-199	0.0	8.564943685495268E-4	0.0	0.0	0.0
200-204	0.0	8.564943685495268E-4	0.0	0.0	0.0
205-209	0.0	8.564943685495268E-4	0.0	0.0	0.0
210-214	0.0	8.564943685495268E-4	0.0	0.0	0.0
215-219	0.0	8.564943685495268E-4	0.0	0.0	0.0
220-224	0.0	8.564943685495268E-4	0.0	0.0	0.0
225-229	0.0	8.564943685495268E-4	0.0	0.0	0.0
230-234	0.0	8.564943685495268E-4	0.0	0.0	0.0
235-239	0.0	8.564943685495268E-4	0.0	0.0	0.0
240-244	0.0	8.564943685495268E-4	0.0	0.0	0.0
245-249	0.0	8.564943685495268E-4	0.0	0.0	0.0
250-254	0.0	8.564943685495268E-4	0.0	0.0	0.0
255-259	0.0	8.564943685495268E-4	0.0	0.0	0.0
260-264	0.0	8.564943685495268E-4	0.0	0.0	0.0
265-269	0.0	8.564943685495268E-4	0.0	0.0	0.0
270-274	0.0	8.564943685495268E-4	0.0	0.0	0.0
275-279	0.0	8.564943685495268E-4	0.0	0.0	0.0
280-284	0.0	8.564943685495268E-4	0.0	8.564943685495268E-4	0.0
285-289	0.0	8.564943685495268E-4	0.0	8.564943685495268E-4	0.0
290	0.0	8.564943685495268E-4	0.0	8.564943685495268E-4	0.0
>>END_MODULE
