FastQCFastQC Report
Fri 1 Nov 2024
JVMS2_cleaned_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJVMS2_cleaned_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116755
Sequences flagged as poor quality0
Sequence length16-301
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT1280310.965697400539591No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT110349.450558862575479No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT96688.280587555136824No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT85417.315318401781508No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT84167.208256605712818No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT72706.22671405935506No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT72166.180463363453385No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT22621.9373902616590295No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT22291.9091259474968951No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT17491.4980086505931225No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT15831.355830585413901No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT13691.1725407905443022No Hit
CCTACGGGGGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT13321.1408504989079697No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12691.0868913536893494No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11771.008093871782793No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11220.960986681512569No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT11040.9455697828786777No Hit
CCTACGGGCGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT8350.7151727977388549No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT8150.6980429103678644No Hit
CCTACGGGGGGCAGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT5260.45051603785705113No Hit
CCTACGGGAGGCAGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT5000.42824718427476344No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC3960.3391717699456126No Hit
CCTACGGGCGGCAGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT3560.30491199520363155No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3480.29806004025523536No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC3270.28007365851569527No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT3150.26979572609310093No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC3120.26722624298745234No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2950.2526658387221104No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC2750.23553595135111988No Hit
CCTACGGGTGGCAGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2720.2329664682454713No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC2600.22268853582287695No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2410.20641514282043597No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC2320.19870669350349018No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2210.1892852554494454No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2180.18671577234379683No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC2160.18500278360669778No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT2060.17643783992120252No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1990.1704423793413558No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1890.16187743565586057No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1870.16016444691876153No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1870.16016444691876153No Hit
CCTACGGGCGGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGATGGA1860.15930795255021196No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1560.13361312149372617No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1520.13018714401952805No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1470.12590467217678045No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1460.1250481778082309No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1430.12247869470258232No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1430.12247869470258232No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1410.12076570596548328No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1380.11819622285983471No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1310.11220076227998801No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1280.10963127917433943No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT1250.10706179606869086No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1220.10449231296304226No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1190.10192282985739369No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1170.10020984112029463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)