##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	JVMS1_cleaned_R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346075
Sequences flagged as poor quality	0
Sequence length	15-301
%GC	52
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.163883551253342	34.0	34.0	34.0	20.0	34.0
2	32.09459799176479	34.0	34.0	34.0	20.0	34.0
3	32.708479375857834	34.0	34.0	34.0	34.0	34.0
4	33.236232030629196	34.0	34.0	34.0	34.0	34.0
5	32.61279780394423	34.0	34.0	34.0	34.0	34.0
6	32.853843819981215	34.0	34.0	34.0	34.0	34.0
7	32.496291266344	34.0	34.0	34.0	34.0	34.0
8	32.5482366539045	34.0	34.0	34.0	34.0	34.0
9	32.91791663656722	34.0	34.0	34.0	34.0	34.0
10-14	33.11712085530593	34.0	34.0	34.0	34.0	34.0
15-19	32.987311709789964	34.0	34.0	34.0	34.0	34.0
20-24	32.934816852132315	34.0	34.0	34.0	34.0	34.0
25-29	32.95969370348846	34.0	34.0	34.0	34.0	34.0
30-34	32.56453551713857	34.0	34.0	34.0	31.2	34.0
35-39	32.54423272467865	34.0	34.0	34.0	31.2	34.0
40-44	32.83185759062445	34.0	34.0	34.0	34.0	34.0
45-49	32.78757967407431	34.0	34.0	34.0	34.0	34.0
50-54	32.72414385402765	34.0	34.0	34.0	34.0	34.0
55-59	32.628543198647094	34.0	34.0	34.0	34.0	34.0
60-64	32.58338037371355	34.0	34.0	34.0	34.0	34.0
65-69	32.05695525392574	34.0	34.0	34.0	22.8	34.0
70-74	31.857690779266818	34.0	34.0	34.0	25.6	34.0
75-79	32.572437793429906	34.0	34.0	34.0	34.0	34.0
80-84	32.40948126292108	34.0	34.0	34.0	31.2	34.0
85-89	32.441225310796774	34.0	34.0	34.0	31.2	34.0
90-94	32.41977319576997	34.0	34.0	34.0	31.2	34.0
95-99	31.779996880316798	34.0	34.0	34.0	22.8	34.0
100-104	32.21504148407261	34.0	34.0	34.0	22.8	34.0
105-109	31.82501128664719	34.0	34.0	34.0	20.0	34.0
110-114	32.290233322597444	34.0	34.0	34.0	28.4	34.0
115-119	32.050451361645436	34.0	34.0	34.0	20.0	34.0
120-124	31.875211874072896	34.0	34.0	34.0	20.0	34.0
125-129	31.852094001396505	34.0	34.0	34.0	20.0	34.0
130-134	31.557590403445367	34.0	34.0	34.0	22.8	34.0
135-139	31.629803713997717	34.0	34.0	34.0	20.0	34.0
140-144	31.794694811405964	34.0	34.0	34.0	20.0	34.0
145-149	31.2349051831753	34.0	34.0	34.0	20.0	34.0
150-154	31.34322536490678	34.0	34.0	34.0	20.0	34.0
155-159	31.324283781556716	34.0	34.0	34.0	20.0	34.0
160-164	31.089537582122063	34.0	34.0	34.0	20.0	34.0
165-169	31.31153615984699	34.0	34.0	34.0	20.0	34.0
170-174	30.714520807534647	34.0	31.2	34.0	17.8	34.0
175-179	31.131615322001444	34.0	34.0	34.0	20.0	34.0
180-184	31.29368393170538	34.0	34.0	34.0	20.0	34.0
185-189	30.67296567457823	34.0	34.0	34.0	20.0	34.0
190-194	30.45776832919422	34.0	31.2	34.0	17.8	34.0
195-199	31.164159732903094	34.0	34.0	34.0	20.0	34.0
200-204	30.095449777007747	34.0	31.2	34.0	17.8	34.0
205-209	29.932005248452306	34.0	34.0	34.0	20.0	34.0
210-214	29.639027879944013	34.0	28.4	34.0	17.8	34.0
215-219	29.663141388223885	34.0	25.6	34.0	17.8	34.0
220-224	29.96234961759763	34.0	31.2	34.0	20.0	34.0
225-229	29.183863595927257	34.0	25.6	34.0	15.6	34.0
230-234	29.822217983651267	34.0	28.4	34.0	20.0	34.0
235-239	28.918720888818864	34.0	20.0	34.0	15.6	34.0
240-244	27.435142834452108	34.0	20.0	34.0	15.6	34.0
245-249	27.968633109191984	34.0	20.0	34.0	15.6	34.0
250-254	28.076362155671667	34.0	20.0	34.0	17.8	34.0
255-259	27.199042098491976	34.0	20.0	34.0	15.6	34.0
260-264	27.795551589780178	34.0	20.0	34.0	13.4	34.0
265-269	26.983982171502497	34.0	20.0	34.0	13.4	34.0
270-274	27.10131558889602	34.0	20.0	34.0	15.6	34.0
275-279	25.451576118243658	28.4	20.0	34.0	9.0	34.0
280-284	26.12926517755644	34.0	20.0	34.0	9.0	34.0
285-289	26.197228967543616	31.2	20.0	34.0	13.4	34.0
290-294	24.961412396202824	22.8	20.0	34.0	9.0	34.0
295-299	24.997197065983375	22.8	20.0	34.0	9.0	34.0
300-301	22.117582004908037	20.0	14.5	34.0	9.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.6913499624969113
1101	2	-0.9239308026084103
1101	3	-0.6700455582338947
1101	4	-0.22014451357368614
1101	5	-0.4107515864486544
1101	6	-0.7815546294650559
1101	7	-0.955258038045141
1101	8	-0.6238033179794868
1101	9	-0.44928626531859095
1101	10-14	-0.42245434162633444
1101	15-19	-0.4304150097957802
1101	20-24	-0.46269404071050246
1101	25-29	-0.3836300400755235
1101	30-34	-0.6913136158995137
1101	35-39	-0.5757602579422887
1101	40-44	-0.3911414535773048
1101	45-49	-0.5115951115771651
1101	50-54	-0.43039552046025875
1101	55-59	-0.4795476928974267
1101	60-64	-0.4339981688362329
1101	65-69	-0.985285682889625
1101	70-74	-0.6894855715548225
1101	75-79	-0.5722151964085
1101	80-84	-0.42617379385891496
1101	85-89	-0.5778549061899696
1101	90-94	-0.6402772173499329
1101	95-99	-0.8324959038292299
1101	100-104	-0.7975469217414215
1101	105-109	-0.9547427810704932
1101	110-114	-0.7773222417492036
1101	115-119	-1.001514054701893
1101	120-124	-1.0145374717855802
1101	125-129	-0.9092175491510268
1101	130-134	-0.9923754186802967
1101	135-139	-1.1780274334788068
1101	140-144	-1.0498098241587286
1101	145-149	-1.0466576867463395
1101	150-154	-1.0157689716668088
1101	155-159	-1.3118454056967934
1101	160-164	-1.423598757652968
1101	165-169	-1.10033907577197
1101	170-174	-1.3562775129980302
1101	175-179	-1.4147901640499647
1101	180-184	-1.3170319470891876
1101	185-189	-1.7855175466927022
1101	190-194	-1.5336227971635488
1101	195-199	-1.2811125687801557
1101	200-204	-1.9478758070073
1101	205-209	-2.257446486932711
1101	210-214	-1.6166775937606488
1101	215-219	-2.2812354522532914
1101	220-224	-2.282122262446169
1101	225-229	-2.4470568424505394
1101	230-234	-2.6009116934268803
1101	235-239	-2.8745335625881054
1101	240-244	-2.601945848082053
1101	245-249	-3.0174326562907794
1101	250-254	-3.0536756783664636
1101	255-259	-3.4440610197405803
1101	260-264	-3.295384640253072
1101	265-269	-3.5058691727720834
1101	270-274	-3.5524319391286134
1101	275-279	-3.726631925303458
1101	280-284	-3.822840789667108
1101	285-289	-3.747518918052748
1101	290-294	-4.327556417192962
1101	295-299	-4.162504936520705
1101	300-301	-3.604342439964107
1102	1	-0.2935069775516297
1102	2	-0.734293291955062
1102	3	-0.7085636842110006
1102	4	-0.4021085945247833
1102	5	-0.6053509715174386
1102	6	-0.6454990214448273
1102	7	-0.6677237189799321
1102	8	-0.6571210251451056
1102	9	-0.3963603597746186
1102	10-14	-0.37855900222520944
1102	15-19	-0.4716004546622088
1102	20-24	-0.5164223937000116
1102	25-29	-0.5699932124134932
1102	30-34	-0.647528051192495
1102	35-39	-0.7886352168835558
1102	40-44	-0.5065912277530487
1102	45-49	-0.5854565818618696
1102	50-54	-0.6275769688910273
1102	55-59	-0.5266144081284097
1102	60-64	-0.7440303426964832
1102	65-69	-1.1132741416158467
1102	70-74	-1.2548102077919125
1102	75-79	-0.8566305673993639
1102	80-84	-0.9563466825083928
1102	85-89	-0.8685653549393813
1102	90-94	-0.8954437703008544
1102	95-99	-1.3389113433284088
1102	100-104	-1.1213539122753886
1102	105-109	-1.0990870945927433
1102	110-114	-1.0019874247438771
1102	115-119	-1.187261713557909
1102	120-124	-1.4679653067584084
1102	125-129	-1.2483971470584123
1102	130-134	-1.474736061281618
1102	135-139	-1.58948048684098
1102	140-144	-1.6142361136791976
1102	145-149	-1.8732572123823914
1102	150-154	-1.7958494354627668
1102	155-159	-1.7834354657609666
1102	160-164	-1.9251568214882937
1102	165-169	-1.8772760646141116
1102	170-174	-2.332304681293259
1102	175-179	-2.128949491561311
1102	180-184	-1.8886781381292579
1102	185-189	-2.242489435877623
1102	190-194	-2.3902818700503765
1102	195-199	-1.9395155342601136
1102	200-204	-2.2385634819820375
1102	205-209	-2.442014221834487
1102	210-214	-1.7125482191147405
1102	215-219	-2.300393936045925
1102	220-224	-2.589131129898412
1102	225-229	-2.6849003378882976
1102	230-234	-2.6100297743681473
1102	235-239	-2.828867900808259
1102	240-244	-3.258999836239937
1102	245-249	-3.5461058135704313
1102	250-254	-3.8591260326239194
1102	255-259	-4.05580374727521
1102	260-264	-4.39390164551379
1102	265-269	-4.471876878541181
1102	270-274	-4.404447923420651
1102	275-279	-4.817339530357
1102	280-284	-4.719194245283507
1102	285-289	-5.094052748366778
1102	290-294	-4.803459406080162
1102	295-299	-4.884512524784771
1102	300-301	-4.321822211379832
1103	1	-0.3464946969062268
1103	2	-0.21578743459653893
1103	3	-0.42852015361339113
1103	4	-0.2687621071809261
1103	5	-0.8208985530914212
1103	6	-0.04710472844995195
1103	7	-0.4375377120939632
1103	8	-0.21150979957371163
1103	9	-0.2145206949265699
1103	10-14	-0.2529836289801679
1103	15-19	-0.20828974316789584
1103	20-24	-0.2721983044240517
1103	25-29	-0.18557520854988496
1103	30-34	-0.3562918342247343
1103	35-39	-0.48862772215663597
1103	40-44	-0.3113701696628297
1103	45-49	-0.36169501258434167
1103	50-54	-0.4138835489895669
1103	55-59	-0.4057439544408794
1103	60-64	-0.31551701011336775
1103	65-69	-0.46397371530216347
1103	70-74	-0.700502008139523
1103	75-79	-0.3271129078150494
1103	80-84	-0.42617865029064816
1103	85-89	-0.46202597399789624
1103	90-94	-0.23997490447431602
1103	95-99	-0.5890636206992639
1103	100-104	-0.5115625837337738
1103	105-109	-0.6465845828066712
1103	110-114	-0.40996717588384257
1103	115-119	-0.3204377480182252
1103	120-124	-0.6453284631047111
1103	125-129	-0.5813672993981491
1103	130-134	-0.7845486356404763
1103	135-139	-0.7278879922765178
1103	140-144	-0.7010777062018008
1103	145-149	-0.8612469876082898
1103	150-154	-0.8117328906405668
1103	155-159	-1.0908780202409574
1103	160-164	-1.05092010090096
1103	165-169	-1.0122742134305298
1103	170-174	-1.084128761051943
1103	175-179	-1.1616188982921258
1103	180-184	-1.1196063387631874
1103	185-189	-1.2684299074373513
1103	190-194	-1.144822521291637
1103	195-199	-1.2263337907682796
1103	200-204	-1.8853337452492767
1103	205-209	-1.7645937496953579
1103	210-214	-1.0712104010196235
1103	215-219	-1.8458431276081981
1103	220-224	-1.8977895566974823
1103	225-229	-2.328836354499412
1103	230-234	-2.2609308991007104
1103	235-239	-2.861402596351006
1103	240-244	-3.0106462251979096
1103	245-249	-3.0614862273294854
1103	250-254	-3.2697221424102274
1103	255-259	-3.115921785989208
1103	260-264	-3.4066514720963177
1103	265-269	-3.3584909219174826
1103	270-274	-3.649586770539212
1103	275-279	-3.8000789298746938
1103	280-284	-4.0111578768180784
1103	285-289	-4.281577204029954
1103	290-294	-4.5717372776526695
1103	295-299	-4.690634706662042
1103	300-301	-2.293795423810657
1104	1	-0.2522174059316029
1104	2	-1.235483596522002
1104	3	-0.7077909969144187
1104	4	-0.06938090157988341
1104	5	-0.49658512780469266
1104	6	-0.6681136640597956
1104	7	-0.46905612783448447
1104	8	-0.7205912225274282
1104	9	-0.2868553907011844
1104	10-14	-0.6613309740423432
1104	15-19	-0.5046298967959757
1104	20-24	-0.5521181499578063
1104	25-29	-0.5242676926232122
1104	30-34	-0.7728712408564995
1104	35-39	-0.8376678617726903
1104	40-44	-0.550315604702547
1104	45-49	-0.7291573258684849
1104	50-54	-0.659249605981131
1104	55-59	-0.6542510389725358
1104	60-64	-0.6364396381865234
1104	65-69	-0.9710484963996606
1104	70-74	-1.2162544327083253
1104	75-79	-0.9507205076005363
1104	80-84	-0.9477491704775787
1104	85-89	-1.0178606480191945
1104	90-94	-0.9898108717706364
1104	95-99	-1.2937590882376604
1104	100-104	-1.174390322371707
1104	105-109	-1.436265938426697
1104	110-114	-1.176611279581408
1104	115-119	-1.4139073801036872
1104	120-124	-1.494834480931381
1104	125-129	-1.4746963728016347
1104	130-134	-1.5344375448597063
1104	135-139	-1.637184560839902
1104	140-144	-1.5294726591497074
1104	145-149	-1.842126953105879
1104	150-154	-1.9473188567872413
1104	155-159	-1.9801896124753924
1104	160-164	-2.2426655582350286
1104	165-169	-2.25239041034445
1104	170-174	-2.3014891112488023
1104	175-179	-2.4903579440226444
1104	180-184	-2.113991390070364
1104	185-189	-2.4969371988476006
1104	190-194	-2.5717725660768735
1104	195-199	-2.2633453188374624
1104	200-204	-2.892802535834946
1104	205-209	-3.0232178012108193
1104	210-214	-2.341278555079505
1104	215-219	-3.038100820190806
1104	220-224	-2.8498218489780776
1104	225-229	-3.3200445098949842
1104	230-234	-3.162325434966654
1104	235-239	-3.621987974559236
1104	240-244	-3.743885070427673
1104	245-249	-4.212154862976185
1104	250-254	-4.396747656009552
1104	255-259	-4.496517279739788
1104	260-264	-4.783654467748704
1104	265-269	-4.800509889358949
1104	270-274	-5.387356991574311
1104	275-279	-5.142019356260377
1104	280-284	-5.339570835968107
1104	285-289	-5.64313933955065
1104	290-294	-5.578587065758462
1104	295-299	-5.50555390615256
1104	300-301	-4.986301346872608
1201	1	-0.9364549832840439
1201	2	-0.5651917302637699
1201	3	-0.4776170924122738
1201	4	-0.38345065069414375
1201	5	-0.49074643205419477
1201	6	-0.6643057196873059
1201	7	-0.5930804420275884
1201	8	-0.5011558582142825
1201	9	-0.6518675318075609
1201	10-14	-0.3933238744075993
1201	15-19	-0.4819752314347312
1201	20-24	-0.550759819313221
1201	25-29	-0.4821741284492944
1201	30-34	-0.7499404030227481
1201	35-39	-0.631956795551254
1201	40-44	-0.5579486819815074
1201	45-49	-0.6260869635904669
1201	50-54	-0.5361932602756525
1201	55-59	-0.7005755111793093
1201	60-64	-0.9031374800670591
1201	65-69	-0.9865163891787319
1201	70-74	-1.2637230976731715
1201	75-79	-0.9551989203925046
1201	80-84	-0.8123308405823266
1201	85-89	-0.8853442389855246
1201	90-94	-0.9305943979933886
1201	95-99	-1.3711616572015615
1201	100-104	-1.1326152153127218
1201	105-109	-1.4186898459645683
1201	110-114	-1.2611952460592804
1201	115-119	-1.5004446793060602
1201	120-124	-1.544669682703308
1201	125-129	-1.3977291434287693
1201	130-134	-1.4208963090381985
1201	135-139	-1.4925938746294598
1201	140-144	-1.4375131759120734
1201	145-149	-1.6889105410290313
1201	150-154	-1.715586670433833
1201	155-159	-1.8757637847400765
1201	160-164	-1.853121497292971
1201	165-169	-2.0607330659283107
1201	170-174	-1.8610063703991386
1201	175-179	-1.9779782010544515
1201	180-184	-1.8705749562670881
1201	185-189	-1.8801548704563018
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2102	215-219	2.5872553248224577
2102	220-224	2.195183238524585
2102	225-229	2.4169635818023743
2102	230-234	2.5303430449066617
2102	235-239	2.3983997926629925
2102	240-244	2.6763624495959917
2102	245-249	2.845076083721036
2102	250-254	3.1050999245809123
2102	255-259	3.1178722230873746
2102	260-264	2.932851167387202
2102	265-269	3.403972178717005
2102	270-274	3.2564737920176157
2102	275-279	3.5260803478135223
2102	280-284	3.815942425005961
2102	285-289	3.682891033344074
2102	290-294	4.2521869622526935
2102	295-299	4.064919336525421
2102	300-301	2.461523062030622
2103	1	0.9072773666279126
2103	2	0.8312998475295146
2103	3	0.6671061146551978
2103	4	0.20032764284194826
2103	5	0.6316496608392583
2103	6	0.6652432765615828
2103	7	0.6952490231183788
2103	8	0.7210948414609106
2103	9	0.511670570233477
2103	10-14	0.46559502655256324
2103	15-19	0.5493050357470537
2103	20-24	0.6311674397793894
2103	25-29	0.46382122972587325
2103	30-34	0.6948797855929172
2103	35-39	0.6002796812182609
2103	40-44	0.5674709253777124
2103	45-49	0.6377435069678015
2103	50-54	0.565505103378257
2103	55-59	0.6795389499503273
2103	60-64	0.8352698064104587
2103	65-69	0.9557260897098985
2103	70-74	1.1218293073379115
2103	75-79	0.8508191394978084
2103	80-84	0.8644889689837854
2103	85-89	0.8456798289710648
2103	90-94	0.7948571984073283
2103	95-99	1.1116190815314333
2103	100-104	0.8790798483842934
2103	105-109	1.1190580564000463
2103	110-114	0.939933663170315
2103	115-119	1.0274840179442393
2103	120-124	1.0613469516334035
2103	125-129	1.0158328795319953
2103	130-134	1.210082018883817
2103	135-139	1.255430389947076
2103	140-144	1.1086410192472655
2103	145-149	1.3624282472249618
2103	150-154	1.3038667454777162
2103	155-159	1.3606867328609056
2103	160-164	1.3823943589673995
2103	165-169	1.3922316078245274
2103	170-174	1.6327782750876096
2103	175-179	1.5435253831949147
2103	180-184	1.3959673225793878
2103	185-189	1.5809317519372996
2103	190-194	1.5630830043676909
2103	195-199	1.3148604691795676
2103	200-204	1.849245368739986
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2103	210-214	2.3954002586867915
2103	215-219	2.0967902469821738
2103	220-224	1.9612784550749751
2103	225-229	1.7518371167230846
2103	230-234	2.1922932860596767
2103	235-239	2.1769537801917096
2103	240-244	2.4358609727335008
2103	245-249	2.9824943614767285
2103	250-254	2.8007026181836068
2103	255-259	2.896676690525986
2103	260-264	3.062522845590184
2103	265-269	3.280559402486883
2103	270-274	3.371959449606429
2103	275-279	3.432517288213429
2103	280-284	3.8688133917154737
2103	285-289	3.799633004897583
2103	290-294	3.7638574944748378
2103	295-299	3.846252184719159
2103	300-301	3.0772977061939244
2104	1	0.8065795412465455
2104	2	0.5876521450210461
2104	3	0.7636772650238157
2104	4	0.2810528248329547
2104	5	0.6832678738498714
2104	6	0.31709564360382103
2104	7	0.6484182167939068
2104	8	0.7947374435845305
2104	9	0.5428292849364951
2104	10-14	0.4461370583604207
2104	15-19	0.5609362315554591
2104	20-24	0.6560758646878142
2104	25-29	0.49810045261268954
2104	30-34	0.7351681891724624
2104	35-39	0.730555102000018
2104	40-44	0.5604509411425269
2104	45-49	0.6111079062815676
2104	50-54	0.6004218743709799
2104	55-59	0.5995510054054165
2104	60-64	0.7142191271319334
2104	65-69	1.0006535796932283
2104	70-74	1.015757340089472
2104	75-79	0.75440241896343
2104	80-84	0.7838791866092976
2104	85-89	0.6955699388611762
2104	90-94	0.7289339060134452
2104	95-99	1.06022498543145
2104	100-104	0.9529021757818796
2104	105-109	1.0123471874990209
2104	110-114	0.9391426059033492
2104	115-119	1.1625300748386422
2104	120-124	1.1899724058488488
2104	125-129	1.1591920251482044
2104	130-134	1.2557827350291078
2104	135-139	1.30019071832589
2104	140-144	1.2851166861107828
2104	145-149	1.4069529907956309
2104	150-154	1.373375648069942
2104	155-159	1.5226998417791506
2104	160-164	1.5228597563684296
2104	165-169	1.4830576128679596
2104	170-174	1.7105108468282566
2104	175-179	1.6797131072999
2104	180-184	1.5102760594467597
2104	185-189	1.8647786134380198
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2104	250-254	2.8948984024385673
2104	255-259	3.2158628696605085
2104	260-264	3.1096877690865554
2104	265-269	3.0060786997822113
2104	270-274	3.3106430003763627
2104	275-279	3.297557729253999
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2104	290-294	3.8163528267374254
2104	295-299	4.090589572480436
2104	300-301	3.330488055854296
2201	1	0.8061110766245214
2201	2	0.7370364486606746
2201	3	0.5392081633576709
2201	4	0.13648447304906597
2201	5	0.663113729505163
2201	6	0.43117228543254527
2201	7	0.7795307059082432
2201	8	0.5713289525997212
2201	9	0.5364249584581628
2201	10-14	0.39580498217265614
2201	15-19	0.507447737317662
2201	20-24	0.5159830866671911
2201	25-29	0.4258288142911013
2201	30-34	0.5461070498800495
2201	35-39	0.6184849797078726
2201	40-44	0.5099682446838827
2201	45-49	0.6075101318697378
2201	50-54	0.5586900876391923
2201	55-59	-0.13199531194521086
2201	60-64	-0.8609982341231692
2201	65-69	0.37044613449358366
2201	70-74	0.46335382860728913
2201	75-79	0.3543172549629148
2201	80-84	0.41433367998003234
2201	85-89	0.2918609916809842
2201	90-94	0.34197086905040663
2201	95-99	0.7926219309582017
2201	100-104	0.5934681929809287
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2202	1	0.5357242148102479
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2204	2	0.8318632019024079
2204	3	0.3564326023295621
2204	4	0.34310430650296553
2204	5	0.7925965560668402
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2204	7	0.6171579239532292
2204	8	0.5423669711868087
2204	9	0.3701191612726973
2204	10-14	0.39485151375061633
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2204	75-79	0.7373927831639904
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2301	1	-1.879176231525804
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2301	3	0.37041537038070516
2301	4	0.21394497871958862
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2301	6	0.33851713187613797
2301	7	0.6004854865407339
2301	8	0.5994396474580057
2301	9	0.34648419356378213
2301	10-14	0.3746609285970024
2301	15-19	0.401424564585227
2301	20-24	0.5009911703766008
2301	25-29	0.3760718366614242
2301	30-34	0.6030025647416366
2301	35-39	0.5088836794998599
2301	40-44	0.45016136735269185
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2301	55-59	0.584128578370084
2301	60-64	0.40782186847660284
2301	65-69	0.7651546673041807
2301	70-74	0.9283789799358999
2301	75-79	0.5624874820484962
2301	80-84	0.6673286131310121
2301	85-89	0.5800264462092528
2301	90-94	0.5850550940623478
2301	95-99	0.8392319222697147
2301	100-104	0.6473584200744469
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2301	115-119	0.8255022403240062
2301	120-124	1.007384726554676
2301	125-129	0.9225394138340697
2301	130-134	1.0452051026319538
2301	135-139	0.9972850936293618
2301	140-144	1.0851655817008066
2301	145-149	1.2088917828591406
2301	150-154	1.2377734278276442
2301	155-159	1.3372182943003068
2301	160-164	1.4340741305939524
2301	165-169	1.4147858448327497
2301	170-174	1.6066610955131466
2301	175-179	1.527442513562594
2301	180-184	1.4820102875246093
2301	185-189	1.6515185251544082
2301	190-194	1.7417652937196806
2301	195-199	1.5293044015056516
2301	200-204	1.9495087283072152
2301	205-209	2.1665798582405813
2301	210-214	-3.2624923404978183
2301	215-219	1.595433001244423
2301	220-224	2.1654842212736085
2301	225-229	2.447016742051627
2301	230-234	2.3774422799320867
2301	235-239	2.703212422957396
2301	240-244	2.7683918710420166
2301	245-249	2.7292530764459677
2301	250-254	3.116781907315527
2301	255-259	3.1249145579217483
2301	260-264	3.1713321938014722
2301	265-269	3.2733081201967202
2301	270-274	3.378244769236698
2301	275-279	3.555905092595932
2301	280-284	3.486421149467521
2301	285-289	3.812558923163042
2301	290-294	3.8508411335207775
2301	295-299	3.961290028870806
2301	300-301	3.1637513758850986
2302	1	1.2131729478399542
2302	2	0.5986411560100606
2302	3	0.4633109646575164
2302	4	0.3396608834410131
2302	5	0.5477367383187399
2302	6	0.5423703688785508
2302	7	0.7372460556217391
2302	8	0.5273381761852605
2302	9	0.3999721420793563
2302	10-14	0.4259905382139024
2302	15-19	0.46478238540161954
2302	20-24	0.51047146908342
2302	25-29	0.3953531998654327
2302	30-34	0.7545821085863835
2302	35-39	0.7082107796556869
2302	40-44	0.6267513074428948
2302	45-49	0.6131225582962117
2302	50-54	0.5647075886566952
2302	55-59	0.6497341555885683
2302	60-64	0.6805194934322927
2302	65-69	1.0270272060668617
2302	70-74	1.1522042265363623
2302	75-79	0.6976258621868823
2302	80-84	0.98149823422834
2302	85-89	0.8616871549783838
2302	90-94	0.8860767631563036
2302	95-99	1.2743275495340178
2302	100-104	0.9532667974113949
2302	105-109	1.1839031750495046
2302	110-114	0.9885560552709691
2302	115-119	1.1236763834643213
2302	120-124	1.3039543593360108
2302	125-129	1.1354634906857264
2302	130-134	1.4601208739544091
2302	135-139	1.419115677159116
2302	140-144	1.3894907829619498
2302	145-149	1.4771674367148222
2302	150-154	1.5728691246236686
2302	155-159	1.4992743528257932
2302	160-164	1.597714629573435
2302	165-169	1.4989301958766106
2302	170-174	1.7164111968832572
2302	175-179	1.632493641581977
2302	180-184	1.5715991507822835
2302	185-189	1.863528087251872
2302	190-194	1.8744972457610487
2302	195-199	1.7079660111959107
2302	200-204	2.1138838112313465
2302	205-209	2.3556546540674432
2302	210-214	2.705269377744507
2302	215-219	2.4429485800238595
2302	220-224	2.259498718046274
2302	225-229	2.5184124559868586
2302	230-234	2.4049999297525737
2302	235-239	2.6433662133467877
2302	240-244	3.056528753482162
2302	245-249	3.4017196288564087
2302	250-254	3.431005425670243
2302	255-259	3.4808204071035256
2302	260-264	3.7424470213114276
2302	265-269	4.012451474907021
2302	270-274	3.924882879907514
2302	275-279	4.1758624828897695
2302	280-284	4.267209083173462
2302	285-289	4.482318962488652
2302	290-294	4.952842216889653
2302	295-299	4.906075811521017
2302	300-301	3.2949100476308573
2303	1	0.7027189823298059
2303	2	0.4325301134945221
2303	3	0.43471689954659354
2303	4	0.1710186642369962
2303	5	0.5709186126242045
2303	6	0.29654783934815754
2303	7	0.4160026922435449
2303	8	0.34682206308487906
2303	9	0.33409981216207996
2303	10-14	0.2926798414200533
2303	15-19	0.3430348445529461
2303	20-24	0.43408343763921664
2303	25-29	0.3568155203165162
2303	30-34	0.5637005182665931
2303	35-39	0.5463410014937793
2303	40-44	0.43260593970202166
2303	45-49	0.5545926986427645
2303	50-54	0.5012265004565037
2303	55-59	0.6026542691828425
2303	60-64	0.6067305482950331
2303	65-69	0.756580237305279
2303	70-74	1.0009612747316083
2303	75-79	0.5838628461766717
2303	80-84	0.6681797175390329
2303	85-89	0.5833318024657288
2303	90-94	0.5736698816482928
2303	95-99	0.8585362023272829
2303	100-104	0.8275009200962202
2303	105-109	0.9131757334769866
2303	110-114	0.7880189867057297
2303	115-119	0.9983366527724868
2303	120-124	0.9212220681530141
2303	125-129	0.9420976546438347
2303	130-134	1.1662338677063886
2303	135-139	1.2093460608105495
2303	140-144	1.0688622886492425
2303	145-149	1.1876600143126694
2303	150-154	1.1936836494784266
2303	155-159	1.2744728307435267
2303	160-164	1.4454687246970543
2303	165-169	1.3595196591410001
2303	170-174	1.6310332693775322
2303	175-179	1.5957184528100719
2303	180-184	1.5349272616491803
2303	185-189	1.6751315269458544
2303	190-194	1.8323109459517894
2303	195-199	1.5246152728493634
2303	200-204	1.7373943257937086
2303	205-209	2.091342813930474
2303	210-214	2.6259793959250572
2303	215-219	2.3021279843836275
2303	220-224	2.018052420413163
2303	225-229	2.401859502336027
2303	230-234	2.3282462428866886
2303	235-239	2.3745827751364423
2303	240-244	2.486503077094735
2303	245-249	2.7471791357034476
2303	250-254	2.9748314846046533
2303	255-259	2.6438323755572632
2303	260-264	3.1529175497901036
2303	265-269	3.1890961752399853
2303	270-274	3.0997392894524687
2303	275-279	3.2453186427121956
2303	280-284	3.563217684727288
2303	285-289	3.6966574092583
2303	290-294	3.9265972960376416
2303	295-299	3.9984222334310218
2303	300-301	2.768467977447365
2304	1	0.8526168372838434
2304	2	0.5610600502471357
2304	3	0.4711692679703603
2304	4	0.3899237518857035
2304	5	0.6647888780981503
2304	6	0.6298889109425474
2304	7	0.7553718692021008
2304	8	0.7524539694828718
2304	9	0.48992259566617236
2304	10-14	0.4612779780467946
2304	15-19	0.45724951423238025
2304	20-24	0.527691118121254
2304	25-29	0.4985030802880459
2304	30-34	0.697642078919074
2304	35-39	0.6534498839857008
2304	40-44	0.62652948158604
2304	45-49	0.7063655285612498
2304	50-54	0.5719076802317815
2304	55-59	0.6756101790748872
2304	60-64	0.7778530422364298
2304	65-69	1.027603590466775
2304	70-74	1.1717925426320264
2304	75-79	0.8482540942489365
2304	80-84	0.8589148739611545
2304	85-89	0.9336719104190223
2304	90-94	0.9267859314389923
2304	95-99	1.1073289231603844
2304	100-104	1.0247822305077179
2304	105-109	1.2108605926319278
2304	110-114	1.0107616682853973
2304	115-119	1.0958799764399885
2304	120-124	1.3105706597023072
2304	125-129	1.1655445615756115
2304	130-134	1.290399235298949
2304	135-139	1.3302770647910798
2304	140-144	1.3290720962564961
2304	145-149	1.4898202863162027
2304	150-154	1.5356700493015119
2304	155-159	1.6674412059556758
2304	160-164	1.685788677937051
2304	165-169	1.4257428205598472
2304	170-174	1.8078282724210837
2304	175-179	1.7253548522899074
2304	180-184	1.5663422359173467
2304	185-189	1.870109495790956
2304	190-194	1.9943063391231632
2304	195-199	1.6611045028310976
2304	200-204	2.117654723265886
2304	205-209	2.4117629918979873
2304	210-214	2.9790046010589464
2304	215-219	2.515492873223238
2304	220-224	2.3570197168039044
2304	225-229	2.5165742206927355
2304	230-234	2.4974631650466854
2304	235-239	2.8647699013169152
2304	240-244	3.118452592705065
2304	245-249	3.4661283833885825
2304	250-254	3.3925305014319207
2304	255-259	3.3269533485505747
2304	260-264	3.481849193911021
2304	265-269	3.944445909421159
2304	270-274	3.7538849710754754
2304	275-279	3.974389569000973
2304	280-284	4.205247668955867
2304	285-289	4.229223137998982
2304	290-294	4.245645950843503
2304	295-299	4.641742396133974
2304	300-301	3.4812509764257697
2401	1	0.2745753934335724
2401	2	1.056146273441648
2401	3	0.3720826464071365
2401	4	0.32708277527466834
2401	5	0.6190845081959182
2401	6	0.6092302859222869
2401	7	0.6885193534023912
2401	8	0.7646556993483742
2401	9	0.3901143528098032
2401	10-14	0.4994930161281417
2401	15-19	0.5315675610103199
2401	20-24	0.5972388683508214
2401	25-29	0.6142015084196331
2401	30-34	0.8019378778171458
2401	35-39	0.7431561039392562
2401	40-44	0.6071515444607769
2401	45-49	0.6409243520906571
2401	50-54	0.6309150331729612
2401	55-59	0.6623748849572451
2401	60-64	0.7460515716482021
2401	65-69	0.943044766937355
2401	70-74	1.022160189690858
2401	75-79	0.7956805648371699
2401	80-84	0.9063413445493964
2401	85-89	0.8441498515954962
2401	90-94	0.8756829902625256
2401	95-99	1.2373075475448196
2401	100-104	1.013442500273392
2401	105-109	1.3210775050880237
2401	110-114	1.1023125565965302
2401	115-119	1.1827337165548073
2401	120-124	1.415922869647062
2401	125-129	1.1919941607523121
2401	130-134	1.5074636784795175
2401	135-139	1.3404005617036567
2401	140-144	1.3780044364800546
2401	145-149	1.7320900409922686
2401	150-154	1.6110763247148974
2401	155-159	1.6868605946789117
2401	160-164	1.8064235044483006
2401	165-169	1.6660677731215188
2401	170-174	1.9880458110494388
2401	175-179	1.9040752033335444
2401	180-184	1.8676000293573054
2401	185-189	2.162526648135767
2401	190-194	2.1908850189822076
2401	195-199	1.865695686078599
2401	200-204	2.3030606598340597
2401	205-209	2.5545344744345755
2401	210-214	2.384442657277745
2401	215-219	2.516787099400794
2401	220-224	2.568999448733585
2401	225-229	2.7486280246866635
2401	230-234	2.6910822501346026
2401	235-239	3.2666914521138892
2401	240-244	3.5667177322101864
2401	245-249	3.435713415485452
2401	250-254	3.6768643088914246
2401	255-259	3.953132242354229
2401	260-264	4.0012006842157035
2401	265-269	4.222499363988856
2401	270-274	4.2394252881992465
2401	275-279	4.450646900034901
2401	280-284	4.499985099927294
2401	285-289	4.8012000334935045
2401	290-294	5.027514590991519
2401	295-299	5.050826193619887
2401	300-301	5.185918708976828
2402	1	0.6802059148729178
2402	2	0.8321576395265424
2402	3	0.434922686269239
2402	4	0.2957048394849693
2402	5	0.5962715368535356
2402	6	0.5680113945195728
2402	7	0.6740592424349288
2402	8	0.5108546597017423
2402	9	0.37341536552257537
2402	10-14	0.4642689264922808
2402	15-19	0.6041596747788986
2402	20-24	0.5588231174350682
2402	25-29	0.5429722474844851
2402	30-34	0.7366333906376639
2402	35-39	0.6985038199487263
2402	40-44	0.5635644942560845
2402	45-49	0.7378963485145533
2402	50-54	0.6394174190035571
2402	55-59	0.7730048048903839
2402	60-64	0.8094605798436021
2402	65-69	0.8911030782545524
2402	70-74	1.2024590587801924
2402	75-79	0.8901747889704552
2402	80-84	0.9127606778647603
2402	85-89	0.8988364248957623
2402	90-94	0.9116706162936907
2402	95-99	1.2549658388340852
2402	100-104	1.0320955763318338
2402	105-109	1.2458943918986556
2402	110-114	1.0234689120444997
2402	115-119	1.1410818621896937
2402	120-124	1.3852806408659646
2402	125-129	1.232770006799587
2402	130-134	1.4594524597997491
2402	135-139	1.4288145715369005
2402	140-144	1.4179607228080329
2402	145-149	1.4025648222039564
2402	150-154	1.5765166494094451
2402	155-159	1.6039121314818487
2402	160-164	1.7561930897017568
2402	165-169	1.6358635706947595
2402	170-174	1.868784829355782
2402	175-179	1.8420450168878482
2402	180-184	1.6588408867538291
2402	185-189	1.9217470430116776
2402	190-194	1.889726603559211
2402	195-199	1.631635735966558
2402	200-204	2.243058123157958
2402	205-209	2.279691351315151
2402	210-214	2.9800620079666658
2402	215-219	2.6481520745184355
2402	220-224	2.252897684963642
2402	225-229	2.4264153566884197
2402	230-234	2.671736977782796
2402	235-239	2.9002999019598477
2402	240-244	3.4921045090137355
2402	245-249	3.6057337276151777
2402	250-254	3.663706788325399
2402	255-259	3.897065347740334
2402	260-264	3.826442906362697
2402	265-269	4.065528579602109
2402	270-274	3.999761792481717
2402	275-279	4.311129617266463
2402	280-284	4.36601844636828
2402	285-289	4.322566086548644
2402	290-294	4.527057657638949
2402	295-299	4.763779625305208
2402	300-301	3.3414022667862184
2404	1	0.6890465292993646
2404	2	0.8345879975427337
2404	3	0.5498930927927788
2404	4	0.3049571675383902
2404	5	0.6663476913983573
2404	6	0.5360823789393336
2404	7	0.9286928921639444
2404	8	0.6086703317680957
2404	9	0.2811091703553501
2404	10-14	0.4929441568023947
2404	15-19	0.6166533119319482
2404	20-24	0.637577197142825
2404	25-29	0.5928185410637496
2404	30-34	0.8312606525673729
2404	35-39	0.8325557807036859
2404	40-44	0.6274599539281169
2404	45-49	0.754713120322748
2404	50-54	0.6946114722390533
2404	55-59	0.7369326065472421
2404	60-64	0.8413982150739585
2404	65-69	1.1542197682730375
2404	70-74	1.3314483702219135
2404	75-79	0.8218421091551775
2404	80-84	0.9901576946589188
2404	85-89	0.8907800874773173
2404	90-94	0.9541003381281001
2404	95-99	1.329289073024146
2404	100-104	1.1067053588499647
2404	105-109	1.4267583199046534
2404	110-114	1.112629315344229
2404	115-119	1.2704809122688587
2404	120-124	1.3321388415204964
2404	125-129	1.1563212995435315
2404	130-134	1.4676793155128465
2404	135-139	1.4817636958071212
2404	140-144	1.501185792835301
2404	145-149	1.6364112714363657
2404	150-154	1.5925175175881883
2404	155-159	1.711065590422841
2404	160-164	1.8157927257345214
2404	165-169	1.6992404520965465
2404	170-174	1.9279689082932663
2404	175-179	1.8851888256881786
2404	180-184	1.8438940209625727
2404	185-189	2.0210349694836225
2404	190-194	2.194801776151472
2404	195-199	1.7820642322642648
2404	200-204	2.4208334151247968
2404	205-209	2.519525061694054
2404	210-214	3.0642995550277945
2404	215-219	2.7471506637279717
2404	220-224	2.4611038106325225
2404	225-229	2.788229837492029
2404	230-234	2.744450669064694
2404	235-239	3.079330332601046
2404	240-244	3.4618911101734753
2404	245-249	3.7329371500178823
2404	250-254	3.681116925188082
2404	255-259	4.032855859262529
2404	260-264	3.8631916237972312
2404	265-269	4.110147486371716
2404	270-274	4.20875362082683
2404	275-279	4.462753086125602
2404	280-284	4.261663572619515
2404	285-289	4.5967974928778155
2404	290-294	4.83778817372535
2404	295-299	4.631391652473351
2404	300-301	3.847382885351358
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	13.0
16	880.0
17	2089.0
18	2232.0
19	2052.0
20	2164.0
21	2426.0
22	2985.0
23	3722.0
24	4815.0
25	6701.0
26	9736.0
27	14803.0
28	22731.0
29	32195.0
30	40859.0
31	46623.0
32	62124.0
33	86839.0
34	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	99.5472079751499	0.16701582027017264	0.037564111825471355	0.24821209275446074
2	0.3996243588817453	98.97738929422813	0.10315683016687135	0.5198295167232536
3	0.31496062992125984	0.3669724770642202	0.19388860796070218	99.12417828505382
4	0.16528209203207397	0.2776854728021383	99.29870692768908	0.258325507476703
5	0.23925449685761757	99.12446723976018	0.3756411182547136	0.26063714512750125
6	0.2144043921115365	0.5065376002311638	0.1733728238098678	99.10568518384744
7	0.35541428881022896	31.869970382142597	37.19829516723254	30.576320161814635
8	34.37029545618724	33.3803366322329	0.3918225818103012	31.85754532976956
9	99.37701365310988	0.16008090731777794	0.19359965325435238	0.2693057863179946
10-14	39.970468829011054	19.959315177345953	39.872050856028316	0.1981651376146789
15-19	0.217706402082399	39.8689369316054	39.75941911054191	20.153937555770288
20-24	19.445712825860028	0.8174605835222418	39.31151357656201	40.42531301405572
25-29	20.07406084295945	0.36059283357871913	41.92368588537448	37.64166043808734
30-34	0.33183007756383537	36.580970874684425	1.007399117008257	62.07979993074348
35-39	19.70384254270134	1.1928162766926753	56.925757708904726	22.177583471701254
40-44	39.09061155950507	18.543387175303085	20.92082605231233	21.445175212879512
45-49	0.8328287200984126	20.0179636528099	20.40898830396166	58.74021932313003
50-54	38.93335616145187	1.2950715557401342	20.71564994776911	39.055922335038886
55-59	38.17559827546706	21.394610718856235	39.56787166438719	0.8619193412895119
60-64	21.365365574026786	35.50888529886914	2.5714964012947235	40.55425272580935
65-69	37.55161207088995	39.87614842474828	1.4369333978876813	21.135306106474086
70-74	41.04490385123064	18.787405129133457	1.3342987954351746	38.83339222420072
75-79	0.6863869205255743	0.2321611352627291	40.217724321852735	58.86372762235895
80-84	20.359073990898676	19.428747506188827	1.2554068843695634	58.95677161854292
85-89	59.51074624197818	0.3944022275949241	39.42710697028751	0.6677445601393756
90-94	0.3499817463915025	0.24934022926996594	76.94525716693549	22.455420857403045
95-99	1.5608926352943908	38.57837844113925	19.984209365239707	39.876519558326656
100-104	0.2635789938345284	19.887145441820078	58.93069870504767	20.918576859297723
105-109	20.23673204277537	39.305083278577285	0.8277023199716953	39.63048235867565
110-114	0.2490898082932322	20.10108851142257	41.85574521837048	37.794076461913725
115-119	19.934632926222612	0.7081239277893748	19.851645567266253	59.50559757872176
120-124	0.4811677297927573	56.12015359236642	2.0380749105730187	41.360603767267804
125-129	57.15637716544588	24.646436460876643	17.57813044994768	0.6190559237297989
130-134	1.7832359540370497	18.062430119768102	39.569465093170734	40.58486883302411
135-139	1.4845320772340536	1.9181143156583382	38.808198455621365	57.78915515148625
140-144	0.6046060346592708	1.1192250060495506	56.09230450644975	42.183864452841426
145-149	19.9330294152729	20.818018636558026	19.44177602965092	39.80717591851815
150-154	0.7302792302323726	38.304269042645025	20.49869235430359	40.46675937281902
155-159	21.216808620880368	70.59015305439283	4.742532121628466	3.450506203098347
160-164	21.062628097633688	37.765744363704115	21.130112260108067	20.04151527855413
165-169	17.956025513285113	4.07238896753654	40.263553423172176	37.708032096006164
170-174	21.707703509538373	37.97611766419106	18.676394349788843	21.639784476481726
175-179	19.045699097018378	2.1924892531320026	39.36045502163357	39.40135662821605
180-184	41.720030465295025	2.0904390370682457	37.60127175707482	18.588258740561905
185-189	22.51462465522186	37.14808148305312	19.80588670059851	20.531407161126513
190-194	55.932437593103835	18.741746372776806	1.970550766548796	23.35526526757057
195-199	19.12576176445377	1.9836343460648318	59.90047424593264	18.990129643548755
200-204	2.058486041181388	38.09357136565239	19.515220032889573	40.33272256027665
205-209	19.72312279567055	38.627615570781956	2.0102667318893284	39.63899490165816
210-214	2.371322468070568	21.077085985810992	73.14457513632173	3.407016409796712
215-219	2.9466300462472157	4.538472198751343	35.248752015165856	57.266145739835586
220-224	36.1456954339724	3.1031414583466175	20.612379923354524	40.138783184326456
225-229	21.18256277553028	2.7172773949545395	18.5118291404919	57.58833068902328
230-234	17.578353569071037	1.1933511119649642	59.29655893862389	21.931736380340105
235-239	18.925115471376987	20.66153773119916	0.4791161055098645	59.93423069191398
240-244	2.2729299034283406	56.21433699661201	20.54998451474853	20.962748585211116
245-249	1.4149980046479964	37.69689429329327	21.307366370102585	39.58074133195615
250-254	38.29710768334556	40.44187208693921	2.5865425326694425	18.674477697045788
255-259	20.3894438548054	19.646851268742587	20.426820309754433	39.53688456669758
260-264	20.23278708191654	18.61343959615111	19.975735663399664	41.17803765853269
265-269	1.3318601599809752	19.583512623710362	20.44475393904147	58.6398732772672
270-274	18.656765661012315	20.086889101509662	59.236827593358065	2.0195176441199623
275-279	21.44138358847899	18.959593592722726	0.9698033537925974	58.62921946500569
280-284	58.42035044868615	0.45444953263459553	19.864019809459858	21.26118020921939
285-289	41.130520635905285	1.5602858708893037	19.79047420615425	37.51871928705117
290-294	21.097114019720944	37.79653815295363	19.257295657499323	21.8490521698261
295-299	37.37719479741155	19.420226263606377	40.60110634674886	2.6014725922332174
300-301	9.74113222339951	1.3612360204867158	0.4848977226566828	88.41273403345708
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.25
2	0.75
3	1.0
4	0.5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.25
10	0.25
11	0.0
12	0.0
13	0.5
14	1.0
15	2.333333333333333
16	3.5
17	3.75
18	3.25
19	3.5
20	4.0
21	3.75
22	3.25
23	3.0
24	5.333333333333333
25	9.0
26	11.75
27	12.5
28	13.833333333333334
29	16.333333333333336
30	21.75
31	20.5
32	13.833333333333334
33	14.583333333333334
34	18.083333333333336
35	18.0
36	21.33333333333333
37	26.416666666666664
38	28.08333333333333
39	30.833333333333325
40	31.666666666666657
41	32.249999999999986
42	41.333333333333336
43	54.83333333333335
44	76.24999999999999
45	100.49999999999996
46	121.08333333333324
47	305.3333333333334
48	477.5833333333329
49	623.3333333333338
50	950.7500000000015
51	2321.9166666666497
52	21782.166666666704
53	64897.41666666678
54	16419.500000000065
55	5293.583333333333
56	2321.0833333333326
57	819.0833333333336
58	199.9166666666667
59	80.16666666666666
60	56.0
61	32.5
62	17.833333333333332
63	17.333333333333332
64	10.0
65	5.083333333333333
66	3.0
67	1.0833333333333333
68	0.5
69	1.0833333333333333
70	1.6666666666666665
71	0.5
72	0.5833333333333333
73	0.5833333333333333
74	0.5
75	1.0
76	1.1666666666666665
77	0.0
78	0.0
79	1.3333333333333333
80	1.25
81	1.3333333333333333
82	1.6666666666666665
83	1.1666666666666665
84	0.5833333333333333
85	0.3333333333333333
86	1.0833333333333333
87	2.25
88	2.4166666666666665
89	1.75
90	1.3333333333333333
91	2.5
92	6.083333333333333
93	6.416666666666666
94	6.499999999999999
95	5.583333333333334
96	9.5
97	17.083333333333336
98	26.583333333333332
99	49.66666666666668
100	162.83333333333354
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.1641612945473594E-4
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	5.828874729904518E-5
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	1.835752235968193
215-219	5.8474129290977795E-5
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	1.7802939265272697E-4
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
10-19	44.0
20-29	62.0
30-39	46.0
40-49	140.0
50-59	375.0
60-69	684.0
70-79	62.0
80-89	56.0
90-99	238.0
100-109	137.0
110-119	129.0
120-129	90.0
130-139	162.0
140-149	213.0
150-159	134.0
160-169	118.0
170-179	164.0
180-189	169.0
190-199	139.0
200-209	419.0
210-219	536.0
220-229	451.0
230-239	438.0
240-249	385.0
250-259	468.0
260-269	615.0
270-279	1000.0
280-289	1250.0
290-299	1964.0
300-302	335387.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	5.14137109008163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.83358624178047	3.9502997905078376
2	8.604507390546843	0.8847793108430254
3	2.7876131062777496	0.4299646030484721
4	1.2083403585679762	0.2485010474608105
5	1.1184173551396617	0.2875099328180308
6	0.6069802731411229	0.18724264971465723
7	0.4046535154274153	0.14563317200028894
8	0.41027370314168493	0.16874954850827134
9	0.31473051199910074	0.14563317200028894
>10	5.693250154555162	6.87307664523586
>50	1.2195807339965155	4.597558332731344
>100	0.6238408362839318	6.218594235353608
>500	0.10678356657112346	3.7110452936502205
>1k	0.016860563142808968	0.9419923427002816
>5k	0.0	0.0
>10k+	0.05058168942842691	71.20941992342699
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	31515	9.106407570613307	No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	30716	8.875532760239832	No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	30354	8.770931156541211	No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	27286	7.884418117460087	No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	26376	7.621469334681788	No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	26178	7.564256302824532	No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	25634	7.407064942570251	No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	24754	7.152784800982446	No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	23625	6.826554937513545	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	1121	0.32391822581810303	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	1093	0.3158274940403092	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	1046	0.30224662284186954	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	960	0.27739651809578847	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	955	0.2759517445640396	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	953	0.27537383515134006	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	925	0.2672831033735462	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	874	0.25254641334970745	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	843	0.24358881745286426	No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	662	0.19128801560355416	No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	629	0.1817525102940114	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	602	0.17395073322256735	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	585	0.1690385032146211	No Hit
GACTACTAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	568	0.16412627320667483	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	568	0.16412627320667483	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	555	0.1603698620241277	No Hit
GACTACCAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	541	0.1563244961352308	No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	534	0.15430181319078234	No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	529	0.15285703965903344	No Hit
GACTACCGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	524	0.15141226612728453	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	524	0.15141226612728453	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	512	0.1479448096510872	No Hit
GACTACACGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	488	0.1410098966986925	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	474	0.13696453080979556	No Hit
GACTACCCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	471	0.13609766669074622	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	447	0.1291627537383515	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	443	0.1280069349129524	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	385	0.11124756194466516	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	369	0.1066242866430687	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	355	0.10257892075417178	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	2.889547063497797E-4	0.0
155-159	0.0	0.0	0.0	2.889547063497797E-4	0.0
160-164	0.0	0.0	0.0	2.889547063497797E-4	0.0
165-169	0.0	0.0	0.0	2.889547063497797E-4	0.0
170-174	0.0	0.0	0.0	2.889547063497797E-4	0.0
175-179	0.0	0.0	0.0	2.889547063497797E-4	0.0
180-184	0.0	0.0	0.0	2.889547063497797E-4	0.0
185-189	0.0	0.0	0.0	4.6232753015964747E-4	0.0
190-194	0.0	0.0	0.0	5.779094126995594E-4	0.0
195-199	0.0	0.0	0.0	5.779094126995594E-4	0.0
200-204	0.0	0.0	0.0	5.779094126995594E-4	0.0
205-209	0.0	0.0	0.0	5.779094126995594E-4	0.0
210-214	0.0	0.0	0.0	5.779094126995594E-4	0.0
215-219	0.0	0.0	0.0	5.779094126995594E-4	0.0
220-224	0.0	0.0	0.0	5.779094126995594E-4	0.0
225-229	0.0	0.0	0.0	5.779094126995594E-4	0.0
230-234	0.0	0.0	0.0	5.779094126995594E-4	0.0
235-239	0.0	0.0	0.0	5.779094126995594E-4	0.0
240-244	0.0	0.0	0.0	5.779094126995594E-4	0.0
245-249	0.0	0.0	0.0	5.779094126995594E-4	0.0
250-254	0.0	0.0	0.0	5.779094126995594E-4	0.0
255-259	0.0	0.0	0.0	5.779094126995594E-4	0.0
260-264	0.0	0.0	0.0	5.779094126995594E-4	0.0
265-269	0.0	0.0	0.0	5.779094126995594E-4	0.0
270-274	0.0	0.0	0.0	5.779094126995594E-4	0.0
275-279	0.0	0.0	0.0	5.779094126995594E-4	0.0
280-284	0.0	0.0	0.0	5.779094126995594E-4	0.0
285-289	0.0	0.0	0.0	5.779094126995594E-4	0.0
290	0.0	0.0	0.0	5.779094126995594E-4	0.0
>>END_MODULE
