##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	JCMS2_cleaned_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127003
Sequences flagged as poor quality	0
Sequence length	16-301
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20917616119304	34.0	34.0	34.0	20.0	34.0
2	33.08185633410234	34.0	34.0	34.0	34.0	34.0
3	33.58909632055936	34.0	34.0	34.0	34.0	34.0
4	33.3961638701448	34.0	34.0	34.0	34.0	34.0
5	33.22837255812855	34.0	34.0	34.0	34.0	34.0
6	33.09305292000976	34.0	34.0	34.0	34.0	34.0
7	33.51151547601238	34.0	34.0	34.0	34.0	34.0
8	33.47102824342732	34.0	34.0	34.0	34.0	34.0
9	33.39393557632497	34.0	34.0	34.0	34.0	34.0
10-14	33.33564876420242	34.0	34.0	34.0	34.0	34.0
15-19	33.45185913649558	34.0	34.0	34.0	34.0	34.0
20-24	33.682861687217525	34.0	34.0	34.0	34.0	34.0
25-29	33.59545406648954	34.0	34.0	34.0	34.0	34.0
30-34	33.51838224222193	34.0	34.0	34.0	34.0	34.0
35-39	33.59443252963993	34.0	34.0	34.0	34.0	34.0
40-44	33.30651415419921	34.0	34.0	34.0	34.0	34.0
45-49	33.609621329472716	34.0	34.0	34.0	34.0	34.0
50-54	33.53217979088796	34.0	34.0	34.0	34.0	34.0
55-59	33.305743987103064	34.0	34.0	34.0	34.0	34.0
60-64	33.262338541943436	34.0	34.0	34.0	31.2	34.0
65-69	33.515940431094904	34.0	34.0	34.0	34.0	34.0
70-74	33.58157632496884	34.0	34.0	34.0	34.0	34.0
75-79	33.358884012522914	34.0	34.0	34.0	34.0	34.0
80-84	33.62155783049526	34.0	34.0	34.0	34.0	34.0
85-89	33.57461428587273	34.0	34.0	34.0	34.0	34.0
90-94	33.12879775075932	34.0	34.0	34.0	34.0	34.0
95-99	33.3059517888643	34.0	34.0	34.0	34.0	34.0
100-104	33.27724580160689	34.0	34.0	34.0	34.0	34.0
105-109	33.42313343576931	34.0	34.0	34.0	34.0	34.0
110-114	33.20708198247736	34.0	34.0	34.0	34.0	34.0
115-119	33.1556380064346	34.0	34.0	34.0	34.0	34.0
120-124	33.3275494633277	34.0	34.0	34.0	34.0	34.0
125-129	33.32658600315544	34.0	34.0	34.0	34.0	34.0
130-134	33.17959866090513	34.0	34.0	34.0	34.0	34.0
135-139	33.32597846489449	34.0	34.0	34.0	34.0	34.0
140-144	33.290863972086335	34.0	34.0	34.0	34.0	34.0
145-149	33.371646912000145	34.0	34.0	34.0	34.0	34.0
150-154	33.02044126313693	34.0	34.0	34.0	34.0	34.0
155-159	33.140650503811244	34.0	34.0	34.0	34.0	34.0
160-164	33.268769330080126	34.0	34.0	34.0	34.0	34.0
165-169	33.292734634316844	34.0	34.0	34.0	34.0	34.0
170-174	32.86321192543976	34.0	34.0	34.0	31.2	34.0
175-179	33.23492083077091	34.0	34.0	34.0	34.0	34.0
180-184	33.05105934851342	34.0	34.0	34.0	34.0	34.0
185-189	33.171610665023636	34.0	34.0	34.0	34.0	34.0
190-194	33.16235846864764	34.0	34.0	34.0	34.0	34.0
195-199	32.61873159439186	34.0	34.0	34.0	28.4	34.0
200-204	33.00722407949717	34.0	34.0	34.0	34.0	34.0
205-209	32.82952080311278	34.0	34.0	34.0	34.0	34.0
210-214	32.94180907202389	34.0	34.0	34.0	34.0	34.0
215-219	33.01438527113093	34.0	34.0	34.0	34.0	34.0
220-224	33.03814871416135	34.0	34.0	34.0	34.0	34.0
225-229	33.11687711864542	34.0	34.0	34.0	34.0	34.0
230-234	32.819714408433825	34.0	34.0	34.0	31.2	34.0
235-239	32.94820900350568	34.0	34.0	34.0	34.0	34.0
240-244	32.68428585540986	34.0	34.0	34.0	31.2	34.0
245-249	32.90025137349495	34.0	34.0	34.0	34.0	34.0
250-254	32.35062336518281	34.0	34.0	34.0	31.2	34.0
255-259	32.50330858331215	34.0	34.0	34.0	31.2	34.0
260-264	32.53976050454169	34.0	34.0	34.0	31.2	34.0
265-269	32.81620188608797	34.0	34.0	34.0	34.0	34.0
270-274	32.4258709727557	34.0	34.0	34.0	34.0	34.0
275-279	32.44306396636388	34.0	34.0	34.0	31.2	34.0
280-284	32.171056766795914	34.0	34.0	34.0	25.6	34.0
285-289	31.872543637023995	34.0	34.0	34.0	22.8	34.0
290-294	31.810160300524068	34.0	34.0	34.0	22.8	34.0
295-299	31.670297973270277	34.0	34.0	34.0	20.0	34.0
300-301	31.221555019157158	34.0	34.0	34.0	20.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.2082795260575594
1101	2	0.35199522180285925
1101	3	-0.02422773674310008
1101	4	0.038337223202461246
1101	5	0.03323403479925702
1101	6	-0.1323521319729437
1101	7	-0.1552992835763689
1101	8	-0.06214781065182251
1101	9	0.12168652863380203
1101	10-14	-0.07820222283298506
1101	15-19	-0.057206384159592005
1101	20-24	0.0698586918893156
1101	25-29	0.027036478266467157
1101	30-34	0.010156409594557658
1101	35-39	0.10028311815925406
1101	40-44	-0.181398487538452
1101	45-49	-0.0849448159555024
1101	50-54	-0.10404035959044222
1101	55-59	-0.09889063214745164
1101	60-64	-0.07879679965108721
1101	65-69	-0.08909960287965646
1101	70-74	-0.15870422078793212
1101	75-79	-0.1035219155962892
1101	80-84	-0.017213780913984067
1101	85-89	-0.033301365498040525
1101	90-94	-0.21363533564404236
1101	95-99	-0.11481927255056945
1101	100-104	-0.15362439767527292
1101	105-109	-0.17946081749702358
1101	110-114	-0.2062594733406371
1101	115-119	-0.19640216257776189
1101	120-124	-0.07438097056670046
1101	125-129	-0.003963709311626928
1101	130-134	-0.0788918684260409
1101	135-139	-0.1371847728705191
1101	140-144	0.009930040801791051
1101	145-149	-0.21496897968593487
1101	150-154	-0.038231433055919695
1101	155-159	-0.2627156741900194
1101	160-164	-0.2889960907286735
1101	165-169	-0.21957766351381025
1101	170-174	-0.10582430789455088
1101	175-179	-0.153775060297761
1101	180-184	-0.0420894285526785
1101	185-189	-0.11948935486127965
1101	190-194	-0.2863420700591206
1101	195-199	-0.17636955213223615
1101	200-204	0.18298854051345614
1101	205-209	-0.08625330858474456
1101	210-214	-0.10053233075107926
1101	215-219	-0.193606050873683
1101	220-224	-0.11491417554861982
1101	225-229	-0.08359955049176904
1101	230-234	-0.39631981319544707
1101	235-239	-0.2602574910761959
1101	240-244	-0.2317210983375091
1101	245-249	-0.25807612550959647
1101	250-254	-0.36428104397918304
1101	255-259	-0.43274123499965356
1101	260-264	-0.19141787840996471
1101	265-269	-0.36871647174704236
1101	270-274	-0.348774812805452
1101	275-279	-0.236219437455766
1101	280-284	-0.3983860410728717
1101	285-289	-0.36808845175713856
1101	290-294	-0.7759498667420992
1101	295-299	-0.3654069867188916
1101	300-301	-0.8926443642364852
1102	1	0.10041720434823276
1102	2	0.16151687683797888
1102	3	-0.0022842694490421422
1102	4	-0.18299770876147647
1102	5	0.017149699494027004
1102	6	-0.15127554474766214
1102	7	-0.08445242409374032
1102	8	-0.14338388390228118
1102	9	0.16538117510644668
1102	10-14	-0.08752932753952081
1102	15-19	-0.14164122676611157
1102	20-24	-0.050228629537244274
1102	25-29	0.09790935320001637
1102	30-34	0.05084484887142082
1102	35-39	0.05426471669439792
1102	40-44	0.01459128564620471
1102	45-49	0.0940131146450085
1102	50-54	0.10535425149011957
1102	55-59	0.035001283339589406
1102	60-64	-0.04311519147870513
1102	65-69	-0.03698592876585849
1102	70-74	0.04447380176321758
1102	75-79	0.028799696204231395
1102	80-84	0.050138074822164924
1102	85-89	0.016870120734864713
1102	90-94	-0.09677186136131155
1102	95-99	0.07472035068992255
1102	100-104	0.03928259159336989
1102	105-109	-0.1355093211733518
1102	110-114	-0.01954299535606907
1102	115-119	-0.11603904710804613
1102	120-124	0.05018800964673886
1102	125-129	-0.09466368317199425
1102	130-134	-0.06809051825727153
1102	135-139	-0.09905387014403999
1102	140-144	-0.016815347826337756
1102	145-149	-0.22875365731609065
1102	150-154	-0.10441584247073621
1102	155-159	-0.32496436436719733
1102	160-164	-0.08506605401954204
1102	165-169	-0.1297152943540354
1102	170-174	-0.16913449439513784
1102	175-179	-0.08822254805190965
1102	180-184	-0.2650354786271265
1102	185-189	-0.30751967683335124
1102	190-194	-0.22248181479993434
1102	195-199	-0.261674533436711
1102	200-204	0.041177632331752534
1102	205-209	-0.23790408311337785
1102	210-214	-0.2641164151252582
1102	215-219	-0.10577461739904237
1102	220-224	-0.14555891523198738
1102	225-229	-0.1403736927140926
1102	230-234	-0.24394508453618613
1102	235-239	-0.2027673502631373
1102	240-244	-0.039106112373353596
1102	245-249	-0.08636375237204419
1102	250-254	-0.16939418312157528
1102	255-259	-0.5538539985539721
1102	260-264	-0.36698178818440397
1102	265-269	-0.31511884130730294
1102	270-274	-0.43819153643270425
1102	275-279	-0.39663638892534436
1102	280-284	-0.7106758565205809
1102	285-289	-0.733447303431781
1102	290-294	-0.7117028856530112
1102	295-299	-0.9353046425529818
1102	300-301	-1.1313269493781668
1103	1	-0.02780754298232324
1103	2	-0.18332409843753084
1103	3	-0.1415294016365607
1103	4	0.1536893237286705
1103	5	-0.33772953307606457
1103	6	-0.08661500686152124
1103	7	-0.3421181581198951
1103	8	-0.23634339969055418
1103	9	-0.46606974706565296
1103	10-14	-0.17704226078910779
1103	15-19	-0.2426473365131585
1103	20-24	0.0058485702571928755
1103	25-29	0.03118634743287174
1103	30-34	0.0071210701460699966
1103	35-39	-0.008759064892764457
1103	40-44	-0.21711749722875595
1103	45-49	-0.09189078599317924
1103	50-54	-0.09996870303582028
1103	55-59	-0.18885928959346643
1103	60-64	-0.04854117093096022
1103	65-69	0.10251554506294269
1103	70-74	-0.10298348581942207
1103	75-79	-0.10837684845420625
1103	80-84	8.337792550960899E-4
1103	85-89	-0.1481071279272328
1103	90-94	-0.06471737262251764
1103	95-99	-0.15576472500477934
1103	100-104	-0.1925968764319208
1103	105-109	-0.11414427131506244
1103	110-114	-0.17587080927966525
1103	115-119	-0.24893059816545104
1103	120-124	-0.1613166893101905
1103	125-129	-0.17904625948240493
1103	130-134	-0.191673823313252
1103	135-139	-0.11124897806764267
1103	140-144	-0.14882779369199284
1103	145-149	-0.11637847152179859
1103	150-154	-0.17967009148486568
1103	155-159	-0.10297441902208959
1103	160-164	0.004428445899748112
1103	165-169	-0.09449056359278529
1103	170-174	-0.2550787325705528
1103	175-179	-0.1833750974603916
1103	180-184	-0.2485928893228717
1103	185-189	-0.19103903665624244
1103	190-194	-0.25034773736064864
1103	195-199	-0.28746956606822494
1103	200-204	0.09756326063238419
1103	205-209	-0.23215148297040145
1103	210-214	-0.26184835249121363
1103	215-219	-0.33766932025631746
1103	220-224	-0.22170332405978854
1103	225-229	-0.4005805510956577
1103	230-234	-0.5310273897272424
1103	235-239	-0.5136718079805505
1103	240-244	-0.5569196861574568
1103	245-249	-0.6250222861450752
1103	250-254	-0.7829575897124528
1103	255-259	-0.5828411095757637
1103	260-264	-0.801101066963863
1103	265-269	-0.4753500017326431
1103	270-274	-0.6845392017124112
1103	275-279	-0.4747501198546118
1103	280-284	-0.6258492268395806
1103	285-289	-0.5521851675094496
1103	290-294	-0.7551525078660042
1103	295-299	-0.701992977242643
1103	300-301	-0.9204560696409381
1104	1	0.3705372380108116
1104	2	0.20212236029459518
1104	3	0.05002560343043427
1104	4	0.06121099921566753
1104	5	0.17023691214406966
1104	6	0.07802861435230568
1104	7	0.04024306246337517
1104	8	0.2956912810886365
1104	9	0.15280200161129898
1104	10-14	-0.04831464002567998
1104	15-19	0.04911485242952551
1104	20-24	0.037077035520120205
1104	25-29	0.05443147748579236
1104	30-34	0.017128952141341358
1104	35-39	0.04845633877799571
1104	40-44	0.05155157156979584
1104	45-49	-0.04233780772495521
1104	50-54	0.018385085951905467
1104	55-59	-0.1109041043665826
1104	60-64	0.09573312250446975
1104	65-69	0.0350344055516274
1104	70-74	-0.05047813732262796
1104	75-79	-0.06524319233439968
1104	80-84	0.039323814161072335
1104	85-89	0.00116097251190439
1104	90-94	-0.22368708324069075
1104	95-99	-0.10411175799656291
1104	100-104	-0.08642278279337745
1104	105-109	0.019058130599248102
1104	110-114	-0.11599225945800384
1104	115-119	-0.1449710514590592
1104	120-124	0.007110779198541195
1104	125-129	0.05939541066332765
1104	130-134	-0.03179398352687457
1104	135-139	0.008555959589351403
1104	140-144	-0.14162610103964113
1104	145-149	-0.1461477124141055
1104	150-154	-0.07034698105932335
1104	155-159	-0.1872226723566328
1104	160-164	-0.0114684781543275
1104	165-169	-0.10499777709427605
1104	170-174	-0.11309524649849578
1104	175-179	-0.07264104428917761
1104	180-184	-0.03274586999614115
1104	185-189	-0.12214329216843822
1104	190-194	-0.11667565678719427
1104	195-199	-0.3571592494000413
1104	200-204	0.17113190231643216
1104	205-209	-0.24158801323081036
1104	210-214	-0.05387266970380011
1104	215-219	-0.06948585233144655
1104	220-224	-0.03735526127056943
1104	225-229	-0.11757084235779303
1104	230-234	-0.23549613122076352
1104	235-239	-0.0828170761050373
1104	240-244	-0.23817819264507278
1104	245-249	-0.31313135065828845
1104	250-254	-0.2625102618837616
1104	255-259	-0.32105452033705006
1104	260-264	-0.3271286506677029
1104	265-269	-0.4515318364118883
1104	270-274	-0.5758194848083207
1104	275-279	-0.3074241058812959
1104	280-284	-0.46429401406397375
1104	285-289	-1.0172790791327166
1104	290-294	-0.6858107307214816
1104	295-299	-0.8366773468723281
1104	300-301	-1.147376651523512
1201	1	-0.816734680102229
1201	2	-0.33719673182823584
1201	3	-0.2946561945223891
1201	4	-0.17468130162990292
1201	5	0.02070420186369404
1201	6	-0.35376933847368264
1201	7	-0.29903152587663584
1201	8	-0.5845935431214215
1201	9	-0.31526653421424555
1201	10-14	-0.18211971345692035
1201	15-19	-0.2300885298579658
1201	20-24	-0.26813880782655986
1201	25-29	-0.13113149536689406
1201	30-34	-0.34587950357740027
1201	35-39	-0.31420943781301247
1201	40-44	-0.4112655178828106
1201	45-49	-0.20572899597596006
1201	50-54	-0.3050461557036357
1201	55-59	-0.44940432688549947
1201	60-64	-0.4006754222218376
1201	65-69	-0.3420283447821433
1201	70-74	-0.23306380710455699
1201	75-79	-0.37934644111054894
1201	80-84	-0.23107671988432088
1201	85-89	-0.28029875156464357
1201	90-94	-0.3257787610332983
1201	95-99	-0.5233952470931271
1201	100-104	-0.33241744233944104
1201	105-109	-0.3638613175538481
1201	110-114	-0.6081551433031365
1201	115-119	-0.43169112266511434
1201	120-124	-0.3751055091859712
1201	125-129	-0.3360028981929162
1201	130-134	-0.3297690614084985
1201	135-139	-0.19424332835013303
1201	140-144	-0.404291877630655
1201	145-149	-0.38513302616576794
1201	150-154	-0.30087212569662114
1201	155-159	-0.549710433816351
1201	160-164	-0.49652855826114717
1201	165-169	-0.4379394771387837
1201	170-174	-0.44559190871478904
1201	175-179	-0.46409631798743334
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2404	300-301	1.504202801029404
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	12.0
17	23.0
18	62.0
19	111.0
20	196.0
21	328.0
22	458.0
23	458.0
24	460.0
25	506.0
26	575.0
27	750.0
28	967.0
29	1410.0
30	2283.0
31	3980.0
32	11999.0
33	99388.0
34	3037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.3283387006606143	0.052754659338755776	0.11180838247915403	99.50709825752148
2	0.06929242980204413	0.1314981338288792	0.3070914502590592	99.49211798611002
3	0.1779485523964001	0.1952709778509169	99.45985527900916	0.16692519074352574
4	0.09291119107422659	99.466941725786	0.23778965851200368	0.20235742462776468
5	0.18739714809886382	0.2629859137185736	0.2330653606607718	99.31655157752179
6	99.37954221553822	0.2330653606607718	0.0708644677684779	0.31652795603253464
7	99.81260285190113	0.05826634016519295	0.038581765785060194	0.09054904214861066
8	99.54646740628175	0.12755604198326023	0.10629670165271687	0.21967985008228152
9	30.01503901482642	18.875144681621695	27.808004535325935	23.301811768225946
10-14	59.69181830350464	11.517838161303276	8.550664157539584	20.239679377652497
15-19	24.54945733308977	34.03510484841231	19.9408830069353	21.474554811562616
20-24	59.617958772302806	39.453236956898316	0.6443993007984126	0.28440497000047243
25-29	3.8922466874328743	4.215708198032774	59.73408125277556	32.15796386175879
30-34	3.2507444963960235	57.706777869992266	0.6318179885447556	38.41065964506695
35-39	47.69672449987165	15.6571709342081	19.67063892646621	16.975465639454036
40-44	36.87134912772797	43.8036950882824	0.666980601652726	18.657975182336905
45-49	20.41898587840243	19.935455825513376	35.09766677061775	24.547891525466444
50-54	54.72589643191276	20.53658336824664	18.053066123174137	6.684454076666467
55-59	21.9031538626312	37.79012260121215	1.1048914481559418	39.20183208800071
60-64	39.99105366697275	0.31753182012200404	0.6287634057177779	59.06265110718747
65-69	40.93763535702754	21.82912135077454	35.967522188708365	1.2657211034895535
70-74	40.33797821050161	58.095773618546744	1.420554329627197	0.14569384132444366
75-79	39.675401691981286	21.201114546597218	34.9286554718473	4.194828289574201
80-84	39.41816818590643	1.0006977949364977	39.24080540972219	20.34032860943488
85-89	21.236203020961483	17.883027136355974	42.98293561593558	17.897834226746962
90-94	17.703074172383644	58.60996684021077	3.3755247674482716	20.31143421995731
95-99	17.74220228145987	1.877218326666866	59.95330584826921	20.427273543604052
100-104	35.1011840129102	6.7942452528378965	50.85093540644596	7.253635327805943
105-109	43.16531682954544	40.56193569637569	14.970347711108065	1.302399762970808
110-114	51.10840718774282	45.36533699834035	1.376741810868616	2.149514003048218
115-119	25.192092443996028	50.47278230573992	20.38024372172213	3.954881528541926
120-124	51.94151565463774	21.557312041070855	20.151965955085057	6.349206349206349
125-129	6.530919917618376	37.00076641403389	35.644718208798935	20.823595459548795
130-134	36.8382112833586	14.141348809841192	18.15755307955268	30.862886827247532
135-139	7.1158767675301435	20.835476511295468	60.241182466227194	11.80746425494719
140-144	39.71884153115847	19.324910362589637	19.154788657711343	21.80145944854055
145-149	16.013270997944954	39.52225670100164	36.124956989213366	8.339515311840042
150-154	8.322849001949534	31.975816317671296	7.968144470666235	51.733190209712944
155-159	23.62896109042171	69.06067317817073	0.45323730785563976	6.857128423551921
160-164	24.01048285878926	17.402334962070682	15.448165894397828	43.13901628474223
165-169	37.32810639957309	23.2696965199736	21.85997164446325	17.542225435990062
170-174	4.608352411778637	39.78811083918844	20.01657851109181	35.586958237941104
175-179	45.3962389275733	3.6267032984384127	18.649677103563704	32.32738067042458
180-184	37.822246857081495	37.927418756287025	1.2495874450650535	23.00074694156643
185-189	38.36713419257013	2.9916919383371243	10.27940990649482	48.36176396259793
190-194	22.70970208315891	35.25810033979486	33.70035180062841	8.331845776417826
195-199	18.81830849292388	37.373415021786045	25.536435935757236	18.27184054953284
200-204	72.77365705518058	2.1526866846335473	22.920333579781694	2.1533226804041754
205-209	22.894646607179254	39.922351390769705	5.6414543075700285	31.54154769448101
210-214	43.96255426202851	6.618426729076229	47.585290348272544	1.8337286606227157
215-219	39.99175020505955	19.06793121377874	5.559603188024206	35.38071539313749
220-224	12.14150575092345	19.70471691104996	64.07526067639142	4.0785166616351685
225-229	54.67300651900375	4.179813218845043	21.568038544953303	19.579141717197903
230-234	20.901058143340244	55.08590201463029	20.506764180907254	3.5062756611222046
235-239	56.51175035457404	21.055294577112146	4.541077620487976	17.891877447825834
240-244	37.41726933861334	25.313803230311088	6.174534217480623	31.09439321359495
245-249	50.90725615118406	20.573646549706122	5.448598308226675	23.070498990883152
250-254	26.377235278484378	45.03081615348596	21.909683992052344	6.682264575977322
255-259	17.53830284083358	38.22563395077745	19.036242935886648	25.199820272502325
260-264	38.57007007878127	19.29816788844357	34.82942327805406	7.3023387547211005
265-269	21.690186311028967	52.2642470812749	18.79282352829478	7.252743079401348
270-274	21.07538657040717	3.4807557489044996	26.279349836921174	49.164507843767154
275-279	33.51592955510571	12.37090721463471	19.878983180149824	34.234180050109764
280-284	6.154123775799817	36.47693113770085	21.793968711762293	35.574976374737034
285-289	50.26660924963111	34.9649165025426	9.022284986922848	5.7461892609034315
290-294	26.18446283713705	21.027154644823455	20.911879226831303	31.87650329120819
295-299	40.47021521144624	22.222345527327494	18.84602274979192	18.461416511434347
300-301	12.444472954119025	86.62259249162084	0.7721667174495245	0.1607678368106227
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.5
23	0.5
24	0.0
25	0.0
26	0.0
27	1.4999999999999998
28	2.083333333333333
29	0.8333333333333333
30	1.5833333333333333
31	5.0
32	5.499999999999999
33	6.916666666666665
34	2.1666666666666665
35	0.5833333333333333
36	1.3333333333333333
37	2.666666666666667
38	1.8333333333333333
39	1.5833333333333333
40	1.5833333333333333
41	1.4166666666666665
42	1.6666666666666665
43	3.3333333333333335
44	6.916666666666665
45	9.416666666666666
46	21.249999999999996
47	36.83333333333332
48	129.0833333333333
49	253.83333333333357
50	1218.2500000000011
51	2834.500000000014
52	21467.833333333278
53	6703.166666666653
54	2783.6666666666674
55	3291.916666666668
56	1311.4999999999995
57	295.4166666666667
58	161.16666666666669
59	88.16666666666666
60	42.33333333333334
61	27.583333333333318
62	16.16666666666667
63	7.75
64	9.333333333333334
65	6.25
66	2.5
67	1.5
68	1.3333333333333333
69	1.8333333333333333
70	1.5
71	1.0
72	0.5
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0039369148760265505
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.6293074300304304
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	1.5816853490067807E-4
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
10-19	1.0
20-29	3.0
30-39	4.0
40-49	13.0
50-59	2.0
60-69	1.0
70-79	5.0
80-89	8.0
90-99	55.0
100-109	8.0
110-119	53.0
120-129	53.0
130-139	46.0
140-149	53.0
150-159	14.0
160-169	9.0
170-179	19.0
180-189	45.0
190-199	21.0
200-209	24.0
210-219	27.0
220-229	31.0
230-239	35.0
240-249	38.0
250-259	30.0
260-269	20.0
270-279	30.0
280-289	38.0
290-299	1275.0
300-302	125042.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	5.904584931064621
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.23523136418189	3.792823791563979
2	12.908387785038006	1.5243734399974804
3	5.200693425790106	0.9212380809902129
4	3.1470862781704225	0.7432895285938127
5	2.3336444859314573	0.6889601033046464
6	1.426856914255234	0.5054998700818091
7	1.10681424189892	0.4574695085942852
8	0.8134417922389652	0.38424289190019134
9	0.8801173489798639	0.46770548727195427
>10	6.227496999599947	8.011621772714031
>50	0.6267502333644486	2.455060116690157
>100	0.7601013468462462	10.496602442461988
>500	0.13335111348179757	5.73687235734589
>1k	0.10668089078543806	9.254899490563215
>5k	0.05334044539271903	26.24741147846901
>10k+	0.04000533404453927	28.311929639457333
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	13936	10.972969142461201	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	11492	9.048605151059423	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	10529	8.29035534593671	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	9119	7.180145350897223	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	9077	7.1470752659386	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	7879	6.2037904616426385	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	7260	5.716400399990552	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	2406	1.894443438343976	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1778	1.3999669299150415	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1494	1.1763501649567334	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1450	1.1417053140476996	No Hit
CCTACGGGGGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	1220	0.9606072297504783	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1195	0.9409226553703456	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1154	0.9086399533869279	No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	1057	0.8322638047920128	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	986	0.7763596135524358	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	946	0.7448642945442233	No Hit
CCTACGGGCGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	849	0.6684881459493083	No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	834	0.6566774013212286	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	724	0.5700652740486445	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	667	0.5251844444619419	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	627	0.49368912545372945	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	567	0.4464461469414109	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	546	0.4299111044620993	No Hit
CCTACGGGGGGCAGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	540	0.4251868066108675	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	484	0.3810933599993701	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	476	0.37479429619772764	No Hit
CCTACGGGAGGCAGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	476	0.37479429619772764	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	449	0.35353495586718425	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	436	0.3432989771895152	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	405	0.3188901049581506	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	403	0.31731533900774	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	401	0.31574057305732933	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	397	0.3125910411565081	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	355	0.2795209561978851	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	347	0.2732218923962426	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	342	0.2692849775202161	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	315	0.24802563718967266	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	303	0.23857704148720896	No Hit
CCTACGGGCGGCAGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	300	0.23621489256159303	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	295	0.2322779776855665	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	277	0.2181050841318709	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	274	0.21574293520625498	No Hit
CCTACGGGTGGCAGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	256	0.2015700416525594	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	251	0.19763312677653286	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	246	0.1936962119005063	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	246	0.1936962119005063	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	240	0.1889719140492744	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	240	0.1889719140492744	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	229	0.18031070132201601	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	228	0.1795233183468107	No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	216	0.170074722644347	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	211	0.16613780776832043	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	207	0.1629882758674992	No Hit
CCTACGGGCGGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGATGGA	198	0.1559018290906514	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	196	0.15432706314024078	No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	184	0.14487846743777708	No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	175	0.13779202066092927	No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	175	0.13779202066092927	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	174	0.13700463768572396	No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC	171	0.13464248876010804	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	170	0.1338551057849027	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	162	0.12755604198326023	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	158	0.124406510082439	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	153	0.12046959520641246	No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	152	0.11968221223120715	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	148	0.1165326803303859	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	148	0.1165326803303859	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	146	0.11495791437997527	No Hit
CCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC	145	0.11417053140476997	No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	137	0.10787146760312749	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	134	0.10550931867751155	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	129	0.10157240380148501	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
