##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	JCMS1_cleaned_R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	160867
Sequences flagged as poor quality	0
Sequence length	15-301
%GC	52
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.146182871564708	34.0	34.0	34.0	20.0	34.0
2	32.016149987256554	34.0	34.0	34.0	20.0	34.0
3	32.61609901347075	34.0	34.0	34.0	34.0	34.0
4	33.237861090217386	34.0	34.0	34.0	34.0	34.0
5	32.54988282245582	34.0	34.0	34.0	34.0	34.0
6	32.82210148756426	34.0	34.0	34.0	34.0	34.0
7	32.43666507114573	34.0	34.0	34.0	34.0	34.0
8	32.525813249454515	34.0	34.0	34.0	34.0	34.0
9	32.9322670280418	34.0	34.0	34.0	34.0	34.0
10-14	33.05807033139177	34.0	34.0	34.0	34.0	34.0
15-19	33.002274701290716	34.0	34.0	34.0	34.0	34.0
20-24	32.88503164771127	34.0	34.0	34.0	34.0	34.0
25-29	32.93581391072438	34.0	34.0	34.0	34.0	34.0
30-34	32.529912507463706	34.0	34.0	34.0	31.2	34.0
35-39	32.537369799772605	34.0	34.0	34.0	31.2	34.0
40-44	32.81527678692921	34.0	34.0	34.0	34.0	34.0
45-49	32.78771689192153	34.0	34.0	34.0	34.0	34.0
50-54	32.70586151300498	34.0	34.0	34.0	34.0	34.0
55-59	32.6363971244716	34.0	34.0	34.0	34.0	34.0
60-64	32.5793409827115	34.0	34.0	34.0	34.0	34.0
65-69	32.08129206491587	34.0	34.0	34.0	25.6	34.0
70-74	31.886377149308082	34.0	34.0	34.0	25.6	34.0
75-79	32.58841024731623	34.0	34.0	34.0	34.0	34.0
80-84	32.3989755011145	34.0	34.0	34.0	31.2	34.0
85-89	32.45705232396624	34.0	34.0	34.0	31.2	34.0
90-94	32.4031416881705	34.0	34.0	34.0	31.2	34.0
95-99	31.80444444586438	34.0	34.0	34.0	22.8	34.0
100-104	32.2043865344181	34.0	34.0	34.0	25.6	34.0
105-109	31.80937362137622	34.0	34.0	34.0	20.0	34.0
110-114	32.29279547394946	34.0	34.0	34.0	28.4	34.0
115-119	32.04811038197977	34.0	34.0	34.0	20.0	34.0
120-124	31.882991850218577	34.0	34.0	34.0	20.0	34.0
125-129	31.882781357675704	34.0	34.0	34.0	20.0	34.0
130-134	31.55896390254809	34.0	34.0	34.0	22.8	34.0
135-139	31.635630258497237	34.0	34.0	34.0	20.0	34.0
140-144	31.792652923986633	34.0	34.0	34.0	20.0	34.0
145-149	31.235849936129956	34.0	34.0	34.0	20.0	34.0
150-154	31.319400088864462	34.0	34.0	34.0	20.0	34.0
155-159	31.321635154663078	34.0	34.0	34.0	20.0	34.0
160-164	31.079059042247287	34.0	34.0	34.0	20.0	34.0
165-169	31.321382693785516	34.0	34.0	34.0	20.0	34.0
170-174	30.70610195778907	34.0	31.2	34.0	17.8	34.0
175-179	31.12652749685941	34.0	34.0	34.0	20.0	34.0
180-184	31.337420630180475	34.0	34.0	34.0	20.0	34.0
185-189	30.689409165519557	34.0	34.0	34.0	20.0	34.0
190-194	30.433122812855096	34.0	31.2	34.0	17.8	34.0
195-199	31.151136281946712	34.0	34.0	34.0	20.0	34.0
200-204	30.07974968177764	34.0	31.2	34.0	17.8	34.0
205-209	29.936035253646537	34.0	34.0	34.0	20.0	34.0
210-214	29.611371061936996	34.0	28.4	34.0	17.8	34.0
215-219	29.678436215894997	34.0	25.6	34.0	17.8	34.0
220-224	29.971267413834845	34.0	31.2	34.0	20.0	34.0
225-229	29.21738121138036	34.0	25.6	34.0	15.6	34.0
230-234	29.817741711738382	34.0	28.4	34.0	20.0	34.0
235-239	28.91514790066064	34.0	20.0	34.0	15.6	34.0
240-244	27.45573915531852	34.0	20.0	34.0	15.6	34.0
245-249	27.916442914453576	34.0	20.0	34.0	15.6	34.0
250-254	28.05920230108718	34.0	20.0	34.0	17.8	34.0
255-259	27.173395498057943	34.0	20.0	34.0	15.6	34.0
260-264	27.748096458413478	34.0	20.0	34.0	13.4	34.0
265-269	26.96914012994602	34.0	20.0	34.0	13.4	34.0
270-274	27.06568627042439	34.0	20.0	34.0	15.6	34.0
275-279	25.468234825610278	28.4	20.0	34.0	9.0	34.0
280-284	26.08370466193485	34.0	20.0	34.0	9.0	34.0
285-289	26.174533702281042	31.2	20.0	34.0	13.4	34.0
290-294	24.944287966242662	22.8	20.0	34.0	9.0	34.0
295-299	24.981166673188252	22.8	20.0	34.0	9.0	34.0
300-301	22.164246047686362	20.0	14.5	34.0	9.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-1.2433651711657774
1101	2	-1.166542579765327
1101	3	-0.8220731493806639
1101	4	-0.44541382996228407
1101	5	-0.6554241074276312
1101	6	-0.9503801086822143
1101	7	-1.095008542864111
1101	8	-0.7847209405838989
1101	9	-0.911113115804369
1101	10-14	-0.7193638739498027
1101	15-19	-0.6155230912904344
1101	20-24	-0.6837534626312092
1101	25-29	-0.7645715467961054
1101	30-34	-0.9662528743077914
1101	35-39	-0.885884994001259
1101	40-44	-0.7223094566780404
1101	45-49	-0.6885067639625078
1101	50-54	-0.7450027787495941
1101	55-59	-0.9878791427026279
1101	60-64	-0.8556069757557054
1101	65-69	-1.4549116272237974
1101	70-74	-1.2068535778730016
1101	75-79	-1.1188873513485689
1101	80-84	-1.0815839521299324
1101	85-89	-1.1367530271215607
1101	90-94	-1.010008714762101
1101	95-99	-1.4110104137376211
1101	100-104	-1.2917375224147065
1101	105-109	-1.310396988368094
1101	110-114	-1.164008753516569
1101	115-119	-1.5493918443951884
1101	120-124	-1.387426298020408
1101	125-129	-1.3871779806271114
1101	130-134	-1.3401190750949432
1101	135-139	-1.557188183182518
1101	140-144	-1.6065529965494498
1101	145-149	-1.6818967892777508
1101	150-154	-1.522703111102242
1101	155-159	-1.7126549995104732
1101	160-164	-1.951396217629469
1101	165-169	-1.568810847931939
1101	170-174	-1.9479729875787868
1101	175-179	-1.8877077276405068
1101	180-184	-1.6896892729583115
1101	185-189	-2.1724328644023068
1101	190-194	-2.2798980708215666
1101	195-199	-1.977061479196145
1101	200-204	-2.303199736564995
1101	205-209	-2.441800298442086
1101	210-214	-2.027736958489985
1101	215-219	-2.9663834593785907
1101	220-224	-2.943481354001463
1101	225-229	-3.1822980034066006
1101	230-234	-3.0387822129202533
1101	235-239	-3.2408170417808186
1101	240-244	-3.2442978693900386
1101	245-249	-3.577906061832394
1101	250-254	-3.717552369373305
1101	255-259	-3.9344524557995477
1101	260-264	-3.829076449704253
1101	265-269	-4.105269984780335
1101	270-274	-4.050618393246033
1101	275-279	-4.037928856655125
1101	280-284	-4.402885567136217
1101	285-289	-4.51054944959375
1101	290-294	-4.8235636047093
1101	295-299	-4.61044689801999
1101	300-301	-3.2830117145779205
1102	1	-0.17798749742871323
1102	2	-0.28699432294361316
1102	3	-0.33182815733263027
1102	4	-0.2381087424906525
1102	5	-0.44294756126155477
1102	6	-0.48602814928191407
1102	7	-0.22826563262304944
1102	8	-0.3198471892213419
1102	9	-0.43346975703665436
1102	10-14	-0.35256199406916977
1102	15-19	-0.20863114868755162
1102	20-24	-0.6008064102197039
1102	25-29	-0.435097806155305
1102	30-34	-0.7542545183098639
1102	35-39	-0.5457152329176509
1102	40-44	-0.43841317176533323
1102	45-49	-0.4208432125606407
1102	50-54	-0.6017668538399406
1102	55-59	-0.40406204813464086
1102	60-64	-0.4377719057517666
1102	65-69	-0.8045501269560376
1102	70-74	-0.8489819518882378
1102	75-79	-0.6226283647370039
1102	80-84	-0.7575364253315335
1102	85-89	-0.6656264181414002
1102	90-94	-0.5909850805118104
1102	95-99	-0.9714047484054902
1102	100-104	-0.762313096051102
1102	105-109	-1.1524135655353085
1102	110-114	-0.8815010436241835
1102	115-119	-0.8521630924272827
1102	120-124	-1.2060917857384936
1102	125-129	-0.9465502623958209
1102	130-134	-1.1367571348734842
1102	135-139	-1.384323184785007
1102	140-144	-1.3094320752442634
1102	145-149	-1.4273739889190793
1102	150-154	-1.8352137922192568
1102	155-159	-1.4332590846908282
1102	160-164	-2.101619309039002
1102	165-169	-1.860853308318184
1102	170-174	-1.7822588262733667
1102	175-179	-1.8273104380611187
1102	180-184	-1.8942979271161846
1102	185-189	-2.049906482662081
1102	190-194	-1.9200799108591617
1102	195-199	-1.6471726158056654
1102	200-204	-1.8790214225922348
1102	205-209	-2.2219480665259006
1102	210-214	-1.4864917926272838
1102	215-219	-2.0932712239057842
1102	220-224	-1.8600337217727159
1102	225-229	-2.1476675657995585
1102	230-234	-2.32016711567168
1102	235-239	-2.980871229954804
1102	240-244	-3.462769970017362
1102	245-249	-3.24216540837568
1102	250-254	-3.466112978855815
1102	255-259	-3.7211762227453598
1102	260-264	-3.7591608491377606
1102	265-269	-4.214263472064854
1102	270-274	-4.03128063984672
1102	275-279	-4.5043308966097655
1102	280-284	-4.615376556798804
1102	285-289	-4.8422204801618065
1102	290-294	-4.904229692725419
1102	295-299	-4.954835968512629
1102	300-301	-4.225589074320073
1103	1	-1.1461129047231324
1103	2	-0.36910129725631435
1103	3	-0.4273480977512776
1103	4	-0.29225831174323247
1103	5	-1.0731636152858002
1103	6	-0.7576747287249148
1103	7	-0.38085333967183743
1103	8	-0.3619480048843684
1103	9	-0.4147324234455141
1103	10-14	-0.3228536705825178
1103	15-19	-0.46495637735990414
1103	20-24	-0.46891262221240027
1103	25-29	-0.24183785793537993
1103	30-34	-0.6720437467579394
1103	35-39	-0.5167828418085705
1103	40-44	-0.4100470690920801
1103	45-49	-0.4476356653908269
1103	50-54	-0.5566444645193087
1103	55-59	-0.6532256424381231
1103	60-64	-0.3304473919080593
1103	65-69	-0.607609936947135
1103	70-74	-0.888762061833269
1103	75-79	-0.38762774910279063
1103	80-84	-0.7055971775641119
1103	85-89	-0.42483739236443796
1103	90-94	-0.46359225518533975
1103	95-99	-0.5831952299762975
1103	100-104	-0.7279382544863822
1103	105-109	-0.639734371459646
1103	110-114	-0.4623753982352383
1103	115-119	-0.7420513036250256
1103	120-124	-0.8578092550519081
1103	125-129	-0.7570143618991061
1103	130-134	-0.8610938338113847
1103	135-139	-0.9726374696683884
1103	140-144	-0.7372348147312628
1103	145-149	-1.117655644243925
1103	150-154	-1.0505267102672953
1103	155-159	-1.0092319353695665
1103	160-164	-1.2698823363342875
1103	165-169	-1.116633330305607
1103	170-174	-1.4038833925645449
1103	175-179	-1.414154194690969
1103	180-184	-1.3943694975176015
1103	185-189	-1.6206051932937697
1103	190-194	-1.8138781698555917
1103	195-199	-1.4170176562922379
1103	200-204	-1.9061959874766927
1103	205-209	-1.9913819384657856
1103	210-214	-1.3875248902898427
1103	215-219	-1.9041246378941565
1103	220-224	-2.0967449685873554
1103	225-229	-2.4341631840357607
1103	230-234	-2.327587494024172
1103	235-239	-2.4225778528167545
1103	240-244	-2.887475852370301
1103	245-249	-3.1714215156439884
1103	250-254	-3.331057063021074
1103	255-259	-3.1621391839758743
1103	260-264	-3.8258348825685147
1103	265-269	-3.430149144572791
1103	270-274	-3.609417563326744
1103	275-279	-4.096316177896927
1103	280-284	-4.129018299935574
1103	285-289	-4.476587986583887
1103	290-294	-4.784561603720807
1103	295-299	-4.772578400292161
1103	300-301	-4.158182671079171
1104	1	-0.07282497352813877
1104	2	-1.1282945141482017
1104	3	-0.5880423064722109
1104	4	-0.19030759526312124
1104	5	-0.7813796836324869
1104	6	-0.30438378981728675
1104	7	-0.4902159194299358
1104	8	-0.2739580878829102
1104	9	-0.06635694632920064
1104	10-14	-0.34912031146879485
1104	15-19	-0.5631786677896926
1104	20-24	-0.41706173061559326
1104	25-29	-0.31992085033748197
1104	30-34	-0.7167674301140039
1104	35-39	-0.6357550122631608
1104	40-44	-0.42687349864459634
1104	45-49	-0.7534102623690657
1104	50-54	-0.5954285316052648
1104	55-59	-0.672261281851128
1104	60-64	-0.4462010245257204
1104	65-69	-0.964320241898573
1104	70-74	-1.0443842507388155
1104	75-79	-0.7689885179937157
1104	80-84	-1.0065785709254023
1104	85-89	-1.0794195215896814
1104	90-94	-1.3201038544581678
1104	95-99	-1.381749575991698
1104	100-104	-1.404075548158385
1104	105-109	-1.5801417040252446
1104	110-114	-1.331377129253795
1104	115-119	-1.6387413643917448
1104	120-124	-1.624664233359855
1104	125-129	-1.425462022126954
1104	130-134	-1.897024378091288
1104	135-139	-1.727735867439197
1104	140-144	-1.6850970578521327
1104	145-149	-1.8626965707503658
1104	150-154	-1.9097308203334826
1104	155-159	-2.0769578763206553
1104	160-164	-2.3098155955111466
1104	165-169	-2.3401336611016212
1104	170-174	-2.7421219582911753
1104	175-179	-2.496201785696222
1104	180-184	-2.347686545508477
1104	185-189	-2.710019720691676
1104	190-194	-2.6513524701712257
1104	195-199	-2.629333836787474
1104	200-204	-2.690394433132152
1104	205-209	-2.944066630088244
1104	210-214	-2.41334927622896
1104	215-219	-3.0598100328051743
1104	220-224	-3.4737960393161487
1104	225-229	-3.4406952740749333
1104	230-234	-3.415045700258535
1104	235-239	-3.743985440731919
1104	240-244	-4.265043485875296
1104	245-249	-4.468190643090455
1104	250-254	-4.8904549534567145
1104	255-259	-4.486905694479123
1104	260-264	-4.961922728475162
1104	265-269	-5.121599232714814
1104	270-274	-5.705029467812263
1104	275-279	-5.220098216424514
1104	280-284	-5.4006557792071845
1104	285-289	-5.523329790357934
1104	290-294	-5.578104816573575
1104	295-299	-5.033438886177986
1104	300-301	-5.281916243545588
1201	1	-0.9188777548025655
1201	2	-0.5491317773903113
1201	3	-0.6628322933704744
1201	4	-0.44543176814730145
1201	5	-0.18905282287420277
1201	6	-0.2734791095253044
1201	7	-0.6515837845811561
1201	8	-0.39575651200478035
1201	9	-0.3200535072155617
1201	10-14	-0.4608028751516642
1201	15-19	-0.4095882302426759
1201	20-24	-0.7536794525831283
1201	25-29	-0.5647905156974815
1201	30-34	-0.6905299708089778
1201	35-39	-0.7133511852749166
1201	40-44	-0.6370743712333464
1201	45-49	-0.5183383956049568
1201	50-54	-0.758691216255226
1201	55-59	-0.6330191495454045
1201	60-64	-1.1763186246851731
1201	65-69	-1.1580858831627197
1201	70-74	-1.04112865310411
1201	75-79	-0.9741686832097542
1201	80-84	-0.9403681308660481
1201	85-89	-0.7132267990684795
1201	90-94	-0.8451475946304718
1201	95-99	-1.4300032911379787
1201	100-104	-1.0671721308882098
1201	105-109	-1.0819931867617214
1201	110-114	-1.3205411188363598
1201	115-119	-1.310499822357098
1201	120-124	-1.3101758533542345
1201	125-129	-1.0631992667607513
1201	130-134	-1.2271461254544072
1201	135-139	-1.6604153449462693
1201	140-144	-1.1467074367038919
1201	145-149	-1.775800314888599
1201	150-154	-1.8535657883063834
1201	155-159	-1.8336243950561375
1201	160-164	-1.7976068350265244
1201	165-169	-1.698187107636361
1201	170-174	-1.8010227786740174
1201	175-179	-2.0691980728524477
1201	180-184	-2.009965238528178
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2102	210-214	2.862427496018409
2102	215-219	2.4848732251133825
2102	220-224	2.343835936551123
2102	225-229	2.1904660699646854
2102	230-234	2.123077369622436
2102	235-239	2.442925355460183
2102	240-244	2.7219359123355815
2102	245-249	2.90721986029218
2102	250-254	2.8467748271541637
2102	255-259	2.754783892947202
2102	260-264	3.0959441651999953
2102	265-269	3.4614863535570954
2102	270-274	3.1850073164973303
2102	275-279	3.282671904510682
2102	280-284	3.6918959240954763
2102	285-289	3.6922862052161243
2102	290-294	4.097909093959203
2102	295-299	4.32892875886839
2102	300-301	3.4336430217618563
2103	1	0.9716518211876632
2103	2	0.9922368028458308
2103	3	0.7451280684046679
2103	4	0.5157515622778774
2103	5	0.5405791494401129
2103	6	0.6695682062427366
2103	7	0.9286887100387489
2103	8	0.7102132140465187
2103	9	0.4250062554785572
2103	10-14	0.5157784370102689
2103	15-19	0.6530424708732596
2103	20-24	0.6689405334039122
2103	25-29	0.6773797417907019
2103	30-34	0.9327884737522467
2103	35-39	0.9111101914985937
2103	40-44	0.7602259440582984
2103	45-49	0.739005272287848
2103	50-54	0.7247734885967603
2103	55-59	0.8441528595630245
2103	60-64	0.8429316818483699
2103	65-69	1.0684993331623787
2103	70-74	1.1946700857293209
2103	75-79	0.9177103674120701
2103	80-84	0.9829546927457997
2103	85-89	0.871133421635669
2103	90-94	0.9226904680775334
2103	95-99	1.3797795079511772
2103	100-104	1.0502696277385155
2103	105-109	1.1799375366574445
2103	110-114	1.086591348628481
2103	115-119	1.2484710644838088
2103	120-124	1.3066806887394975
2103	125-129	1.272516850549291
2103	130-134	1.3889411464203327
2103	135-139	1.4354708909779248
2103	140-144	1.3126120888395434
2103	145-149	1.6116576499887287
2103	150-154	1.5705028452499228
2103	155-159	1.6294777420497866
2103	160-164	1.689889959680901
2103	165-169	1.4890592693148825
2103	170-174	1.9297029451963716
2103	175-179	1.8598496003754441
2103	180-184	1.5187234816095305
2103	185-189	1.6746509736891504
2103	190-194	1.7344288409163369
2103	195-199	1.4612429623180176
2103	200-204	2.0200622055343516
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2103	210-214	2.520514426608134
2103	215-219	2.0840054103068475
2103	220-224	2.1338172979169627
2103	225-229	1.877902139449514
2103	230-234	2.1695994004880106
2103	235-239	2.3399103699983144
2103	240-244	2.582486124816011
2103	245-249	3.202803249541418
2103	250-254	3.32367335842979
2103	255-259	3.1994987218445416
2103	260-264	3.3520896956555184
2103	265-269	3.6514583423526155
2103	270-274	3.574194991567353
2103	275-279	3.9128119605330944
2103	280-284	3.914439341462426
2103	285-289	4.039838025944412
2103	290-294	3.943567357264527
2103	295-299	4.085363735003035
2103	300-301	3.8997757207576527
2104	1	1.1655752202454153
2104	2	0.9191983569666959
2104	3	0.9244997603723277
2104	4	0.3357582510465562
2104	5	0.6305249122505572
2104	6	0.46548442214455577
2104	7	0.9357482691616497
2104	8	0.7002127778224718
2104	9	0.20969697263088705
2104	10-14	0.5115579693780887
2104	15-19	0.5855581190127026
2104	20-24	0.567547759511136
2104	25-29	0.6098389492499052
2104	30-34	0.8121008280646009
2104	35-39	0.7004342008226061
2104	40-44	0.5901851515175025
2104	45-49	0.6653106335932009
2104	50-54	0.6586312974545621
2104	55-59	0.6380805984907596
2104	60-64	0.8663232902399756
2104	65-69	1.0685459532090107
2104	70-74	1.1467563328155634
2104	75-79	0.8657698079715104
2104	80-84	0.8793999141910191
2104	85-89	0.9806497386519979
2104	90-94	0.9006683235553865
2104	95-99	1.109220067391739
2104	100-104	1.0894537769226602
2104	105-109	1.2688827581026665
2104	110-114	0.9743361038732345
2104	115-119	1.1435943267370448
2104	120-124	1.2102444020660243
2104	125-129	1.0034342348727527
2104	130-134	1.2718188285105363
2104	135-139	1.2957949312640054
2104	140-144	1.1607546957582429
2104	145-149	1.5061947202278319
2104	150-154	1.4807703645268866
2104	155-159	1.553456492602784
2104	160-164	1.7443136982234897
2104	165-169	1.5319457134692733
2104	170-174	1.9846005186206561
2104	175-179	1.7236374276450839
2104	180-184	1.563298328143329
2104	185-189	1.962903451633526
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2104	215-219	2.4149345469394135
2104	220-224	2.3624944269743082
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2104	230-234	2.3744968563360374
2104	235-239	2.597379136972787
2104	240-244	2.7175434526489965
2104	245-249	2.915788260625291
2104	250-254	2.697014815041207
2104	255-259	3.1299036438116303
2104	260-264	3.21518109014405
2104	265-269	3.161827689317704
2104	270-274	3.4569176589708697
2104	275-279	3.530043239892027
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2104	285-289	3.431274334316889
2104	290-294	3.9384289298005193
2104	295-299	3.703764282148839
2104	300-301	2.4477985035402234
2201	1	1.3788265092447034
2201	2	0.9449105996372751
2201	3	0.9457296726827451
2201	4	0.5067943430265345
2201	5	0.7939436770693433
2201	6	0.9312215039254497
2201	7	1.1029756975610283
2201	8	0.8035510037078382
2201	9	0.6823814782949569
2201	10-14	0.5665404690958269
2201	15-19	0.6472403318358246
2201	20-24	0.7417257383949973
2201	25-29	0.6997861589029881
2201	30-34	0.9268972081551041
2201	35-39	0.9025005658301524
2201	40-44	0.7621911847000078
2201	45-49	0.7368038462628874
2201	50-54	0.731761010878401
2201	55-59	0.10620435171413334
2201	60-64	-0.8159374977525147
2201	65-69	0.7333114993543859
2201	70-74	0.7189017746383186
2201	75-79	0.5566476063219596
2201	80-84	0.5556454957429366
2201	85-89	0.46493605408710437
2201	90-94	0.5695018532641924
2201	95-99	0.9853991618202897
2201	100-104	0.8208527232870608
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2201	130-134	1.1843158568684977
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2201	140-144	1.2341549680523158
2201	145-149	1.5461763678694425
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2202	2	0.6664792270882529
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2202	4	0.13317580470211965
2202	5	0.48615995752091834
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2202	10-14	0.4492380329698591
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2204	1	1.0787883616899592
2204	2	0.7021471907717824
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2204	4	0.1722631764743099
2204	5	0.5770061627640146
2204	6	0.39591975676744084
2204	7	0.6025331859260277
2204	8	0.4175664153199534
2204	9	0.3640096159313302
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2204	60-64	0.7506935106628276
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2204	90-94	0.8259134877479255
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2301	1	-1.7460754515396104
2301	2	0.8798125604215912
2301	3	0.5127492805258811
2301	4	0.4955394410657519
2301	5	0.8254730888340518
2301	6	0.8489469941215262
2301	7	0.7601129524629897
2301	8	0.6685313958646972
2301	9	0.5975971645694642
2301	10-14	0.43458752791935495
2301	15-19	0.4561030769338643
2301	20-24	0.5620708364342164
2301	25-29	0.6389312569422145
2301	30-34	0.7718854434492215
2301	35-39	0.7014495854379845
2301	40-44	0.6366774592098139
2301	45-49	0.7687567874393579
2301	50-54	0.7490159128391838
2301	55-59	0.5828546388792333
2301	60-64	0.5773968151086848
2301	65-69	1.0101257209365215
2301	70-74	0.9833555230989575
2301	75-79	0.8282596865140235
2301	80-84	0.8556144890897954
2301	85-89	0.8058116819718322
2301	90-94	0.8949193357011325
2301	95-99	1.134969055245989
2301	100-104	1.0383404987201388
2301	105-109	1.233944390182554
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2301	115-119	1.2529647433387012
2301	120-124	1.2191716918148146
2301	125-129	1.0931541599780665
2301	130-134	1.2617540758190096
2301	135-139	1.3988812161758588
2301	140-144	1.30095207444446
2301	145-149	1.526936063912693
2301	150-154	1.4973234956457837
2301	155-159	1.52585934252091
2301	160-164	1.7632578390771592
2301	165-169	1.6328729349661621
2301	170-174	1.737061973164689
2301	175-179	1.7408607421030133
2301	180-184	1.7965331640528994
2301	185-189	2.083548660613605
2301	190-194	2.0990619199544014
2301	195-199	1.9965324329449814
2301	200-204	2.486378648461333
2301	205-209	2.4561123669498137
2301	210-214	-3.0424544567627123
2301	215-219	1.8422491631429168
2301	220-224	2.4866439733460837
2301	225-229	2.5861717102454875
2301	230-234	2.6463162948221424
2301	235-239	2.944527873010415
2301	240-244	3.132277234049454
2301	245-249	2.9781760452352977
2301	250-254	3.0577358535400236
2301	255-259	3.4542077612599584
2301	260-264	3.582983622958402
2301	265-269	3.4154442330051715
2301	270-274	3.3908413548345635
2301	275-279	3.2970240371163015
2301	280-284	3.669644054275384
2301	285-289	4.270070721084323
2301	290-294	4.330450027858536
2301	295-299	4.498489767643925
2301	300-301	4.058237909357427
2302	1	0.8531074278378199
2302	2	0.6340538055577767
2302	3	0.39085822994221786
2302	4	0.0594227095482438
2302	5	0.6081345674332752
2302	6	0.668293297623471
2302	7	0.6356071158871188
2302	8	0.6996675826351293
2302	9	0.3328812112620696
2302	10-14	0.2951602905652706
2302	15-19	0.3926633882179047
2302	20-24	0.44750585588946734
2302	25-29	0.3117919573313088
2302	30-34	0.5611430309978616
2302	35-39	0.46127526637184246
2302	40-44	0.4371295886687747
2302	45-49	0.4356870018643164
2302	50-54	0.5663648407144279
2302	55-59	0.6285184452444028
2302	60-64	0.6275552613938231
2302	65-69	0.8267971551320841
2302	70-74	0.9004702751232543
2302	75-79	0.6305654810484356
2302	80-84	0.7569840488063946
2302	85-89	0.5654634405750727
2302	90-94	0.8146767370169243
2302	95-99	1.1204400193148132
2302	100-104	0.8911905557688158
2302	105-109	1.0438106427180465
2302	110-114	0.8499791327193904
2302	115-119	0.8240957474054227
2302	120-124	1.1935182447959463
2302	125-129	1.0054327265771228
2302	130-134	1.0958987681787171
2302	135-139	1.2271976461961387
2302	140-144	1.2424666112936862
2302	145-149	1.4428657461304084
2302	150-154	1.5241524341177275
2302	155-159	1.4013562999245508
2302	160-164	1.4493460001616079
2302	165-169	1.443467871465426
2302	170-174	1.5290071830205285
2302	175-179	1.5920064631205513
2302	180-184	1.5909102426185164
2302	185-189	1.8471594827648765
2302	190-194	1.5891128401260133
2302	195-199	1.5681751096620289
2302	200-204	1.995520074194566
2302	205-209	2.301390312447534
2302	210-214	2.710792735605363
2302	215-219	2.2667481525991704
2302	220-224	2.1086299961868065
2302	225-229	2.2684031856393574
2302	230-234	2.336710041626148
2302	235-239	2.6332345858717154
2302	240-244	2.8615360053372036
2302	245-249	3.3223992759319536
2302	250-254	3.431928667346366
2302	255-259	3.2340724304966137
2302	260-264	3.4815506133128338
2302	265-269	3.680598188014198
2302	270-274	3.6895545896389628
2302	275-279	4.211765284436911
2302	280-284	4.116241529747143
2302	285-289	4.387998207710954
2302	290-294	4.511719235220848
2302	295-299	4.659383054795331
2302	300-301	4.964928875251726
2303	1	0.7807872935584292
2303	2	0.044910599637276505
2303	3	0.31239633934940514
2303	4	0.18914728420300975
2303	5	0.3076691672654235
2303	6	0.06259405294505171
2303	7	0.5735639328551514
2303	8	0.4094333566490107
2303	9	0.2784599096675038
2303	10-14	0.3614424298801353
2303	15-19	0.17743641026719104
2303	20-24	0.4503531893753845
2303	25-29	0.28841360988339204
2303	30-34	0.5759306840208822
2303	35-39	0.5077018447700112
2303	40-44	0.4117477931980176
2303	45-49	0.439274665631892
2303	50-54	0.4902732802262193
2303	55-59	0.4791248783759059
2303	60-64	0.600227627552961
2303	65-69	0.7211173601910232
2303	70-74	0.9317555771861308
2303	75-79	0.6802907389069759
2303	80-84	0.6965791494940206
2303	85-89	0.660529593571141
2303	90-94	0.7021161095611319
2303	95-99	0.8895223191674688
2303	100-104	0.816020952379823
2303	105-109	0.8729087517553786
2303	110-114	0.7193942218918465
2303	115-119	1.0480734303106445
2303	120-124	1.167200792132654
2303	125-129	0.9256843862961297
2303	130-134	1.0893434833428834
2303	135-139	1.1191479884632258
2303	140-144	0.9575495675531087
2303	145-149	1.2801949026450785
2303	150-154	1.216798912827553
2303	155-159	1.32407819341568
2303	160-164	1.3404122491464001
2303	165-169	1.4422055440492478
2303	170-174	1.7139822381313259
2303	175-179	1.503961446297243
2303	180-184	1.4034096026698997
2303	185-189	1.6730880350951303
2303	190-194	1.912807441831795
2303	195-199	1.552561092929679
2303	200-204	1.6379674259841792
2303	205-209	2.125847906135899
2303	210-214	2.488702005822322
2303	215-219	2.2576751742954286
2303	220-224	2.081016583958011
2303	225-229	2.0510461836669727
2303	230-234	2.005743572198149
2303	235-239	2.183206130492092
2303	240-244	2.2068042265251613
2303	245-249	2.7084241763381236
2303	250-254	2.7516385004341224
2303	255-259	2.436322354485661
2303	260-264	3.0490756189649275
2303	265-269	2.887626276982086
2303	270-274	2.9161617427648743
2303	275-279	2.7916532752941414
2303	280-284	3.302522910067733
2303	285-289	3.4242942653571724
2303	290-294	3.913840272754836
2303	295-299	3.644571513364351
2303	300-301	2.957806440201349
2304	1	0.9662247433921607
2304	2	0.360436342098005
2304	3	0.19396955343036382
2304	4	0.14380844691370953
2304	5	0.3843346872745954
2304	6	0.6615161949012531
2304	7	0.4427991123070427
2304	8	0.8073579065859491
2304	9	0.21263030297102148
2304	10-14	0.3145578982897206
2304	15-19	0.4160718878500447
2304	20-24	0.46770670388061575
2304	25-29	0.40354490216638084
2304	30-34	0.5461014278546799
2304	35-39	0.5552445172118752
2304	40-44	0.4193589406912963
2304	45-49	0.6072269628779594
2304	50-54	0.39716406098733614
2304	55-59	0.5411890105465389
2304	60-64	0.6438708985770631
2304	65-69	0.8795528507948518
2304	70-74	0.7678359549478131
2304	75-79	0.6534958538554463
2304	80-84	0.7499314781436297
2304	85-89	0.7606624841910588
2304	90-94	0.7678238191319338
2304	95-99	0.9638039630282691
2304	100-104	0.9350250769676549
2304	105-109	1.1286638347258453
2304	110-114	0.9619346943665619
2304	115-119	0.9629458037201033
2304	120-124	1.1474336358342043
2304	125-129	0.9511797739340757
2304	130-134	1.1534495077728018
2304	135-139	1.2790128050082004
2304	140-144	1.2706399859066906
2304	145-149	1.4051934608420211
2304	150-154	1.352277649116001
2304	155-159	1.5705180932968759
2304	160-164	1.64784387884524
2304	165-169	1.424052666787059
2304	170-174	1.6452209199772874
2304	175-179	1.7094310703903481
2304	180-184	1.4570480332456448
2304	185-189	1.8363808420477845
2304	190-194	1.864953182709506
2304	195-199	1.6376564763123298
2304	200-204	2.113081454583785
2304	205-209	2.3018649179702244
2304	210-214	2.702894714155658
2304	215-219	2.4235313133486756
2304	220-224	2.1679888164530823
2304	225-229	2.21102060427841
2304	230-234	2.2474110093283173
2304	235-239	2.7113858334013585
2304	240-244	2.6386960226296985
2304	245-249	2.946398781860811
2304	250-254	3.1955171678404355
2304	255-259	3.4169713929472003
2304	260-264	3.387253491156404
2304	265-269	3.6215300970819015
2304	270-274	3.624084097894812
2304	275-279	3.738721940963618
2304	280-284	4.016075349290219
2304	285-289	3.763814542578462
2304	290-294	4.111242427292542
2304	295-299	4.521566379732061
2304	300-301	0.7150580287095174
2401	1	0.16380667026785645
2401	2	0.4791838767280723
2401	3	0.5833543885808865
2401	4	0.23712293811879448
2401	5	0.525528098657233
2401	6	0.41017292732388455
2401	7	0.7681168817360771
2401	8	0.5173997652163678
2401	9	0.32611582861661503
2401	10-14	0.3701207302769234
2401	15-19	0.4886217409810172
2401	20-24	0.5846673000884408
2401	25-29	0.42209903690292094
2401	30-34	0.6452654041564898
2401	35-39	0.761532886027382
2401	40-44	0.4852251998777888
2401	45-49	0.577192150897119
2401	50-54	0.6427290759040147
2401	55-59	0.6842329333857222
2401	60-64	0.7412885312857682
2401	65-69	0.8409601892676406
2401	70-74	0.778513926891435
2401	75-79	0.7706640198500097
2401	80-84	0.767306065014651
2401	85-89	0.807877334239123
2401	90-94	0.7968115908970788
2401	95-99	1.2474790971635414
2401	100-104	0.9696555299829654
2401	105-109	1.1428498126591045
2401	110-114	0.959410155558281
2401	115-119	1.0942539912375793
2401	120-124	1.3148076482501025
2401	125-129	1.1228078180675531
2401	130-134	1.3007949506202614
2401	135-139	1.337360595462286
2401	140-144	1.362798673391616
2401	145-149	1.5047687470164206
2401	150-154	1.5623788681097928
2401	155-159	1.480216810730056
2401	160-164	1.7475585661310227
2401	165-169	1.7440310672741965
2401	170-174	1.885118743558298
2401	175-179	1.722694856356032
2401	180-184	1.7705943598803522
2401	185-189	2.123387380603777
2401	190-194	2.061018537571975
2401	195-199	1.805110276656137
2401	200-204	2.336009376980634
2401	205-209	2.445707010308144
2401	210-214	2.3051510670767037
2401	215-219	2.417170585002218
2401	220-224	2.4907527190121357
2401	225-229	2.8831355156957343
2401	230-234	2.673748976848003
2401	235-239	3.219201409188763
2401	240-244	3.546776116133632
2401	245-249	3.2598584275301015
2401	250-254	3.665758924730202
2401	255-259	3.829508302396018
2401	260-264	3.9112166679018614
2401	265-269	3.783603074261123
2401	270-274	3.9646599334496138
2401	275-279	3.982508248497094
2401	280-284	4.118001835499783
2401	285-289	4.701728759514072
2401	290-294	4.90395884982993
2401	295-299	4.8402142903657115
2401	300-301	4.412857113699477
2402	1	0.9031324692524691
2402	2	0.6651590950750546
2402	3	0.10960076567439359
2402	4	0.12399488661030489
2402	5	0.15212655418058318
2402	6	0.23405590501261742
2402	7	0.7065089920669507
2402	8	0.18126406913202686
2402	9	0.33248825367837753
2402	10-14	0.3429994091074775
2402	15-19	0.36690757166904575
2402	20-24	0.41026448722786313
2402	25-29	0.29610268551364527
2402	30-34	0.5072473482111235
2402	35-39	0.37330355369196155
2402	40-44	0.2910520623844164
2402	45-49	0.42875996204253397
2402	50-54	0.23195242077569134
2402	55-59	0.42615141656158073
2402	60-64	0.5839850729017613
2402	65-69	0.915470607966661
2402	70-74	0.8507141245268386
2402	75-79	0.4845027946667244
2402	80-84	0.6368284200787571
2402	85-89	0.6604256982788499
2402	90-94	0.6570622675835267
2402	95-99	0.9756858878535866
2402	100-104	0.7516597717230908
2402	105-109	0.9790647431156607
2402	110-114	0.8911345440016873
2402	115-119	0.9806191011673917
2402	120-124	1.0835835976616792
2402	125-129	0.8961036032777052
2402	130-134	1.2327677503640366
2402	135-139	1.1801014001430445
2402	140-144	1.2006040611046274
2402	145-149	1.436822788191769
2402	150-154	1.3046708919978904
2402	155-159	1.2868036160279317
2402	160-164	1.5863865022471266
2402	165-169	1.3744950417768855
2402	170-174	1.5755787341061627
2402	175-179	1.542389654170325
2402	180-184	1.4288686825103873
2402	185-189	1.7840130177436144
2402	190-194	1.7114577490613954
2402	195-199	1.4295092658252528
2402	200-204	1.9567338455170642
2402	205-209	2.2275994797232137
2402	210-214	2.630401873020208
2402	215-219	2.4407063631494736
2402	220-224	2.1807580520108445
2402	225-229	2.451630041529409
2402	230-234	2.223803875364183
2402	235-239	2.914624441469087
2402	240-244	3.28822135878508
2402	245-249	3.3338365308647937
2402	250-254	3.4120650388563725
2402	255-259	3.645700227608554
2402	260-264	3.7199157299214995
2402	265-269	4.252604234972612
2402	270-274	4.210613128543262
2402	275-279	4.399140930368038
2402	280-284	4.085829210557478
2402	285-289	4.340079346376967
2402	290-294	4.659507406591029
2402	295-299	4.508248670178116
2402	300-301	3.1935170825198504
2404	1	0.8373088696841009
2404	2	0.9386270919703463
2404	3	0.1691278656119195
2404	4	0.36992185024357127
2404	5	0.6545667790826144
2404	6	0.4470502445995379
2404	7	0.7468777708186352
2404	8	0.28053521995647657
2404	9	0.4938724991774919
2404	10-14	0.5017401091813056
2404	15-19	0.5370667480619673
2404	20-24	0.5097098421703521
2404	25-29	0.5981717923150569
2404	30-34	0.8453630725123205
2404	35-39	0.7755084764513782
2404	40-44	0.569736099679254
2404	45-49	0.6335629177075575
2404	50-54	0.6697055680115938
2404	55-59	0.7622214767695681
2404	60-64	0.86912464597237
2404	65-69	1.1188215205535386
2404	70-74	1.1914759061948743
2404	75-79	0.8575703394937335
2404	80-84	0.9678060857545674
2404	85-89	0.8503655219090476
2404	90-94	1.0205885952223
2404	95-99	1.387256388076814
2404	100-104	0.9909678770260157
2404	105-109	1.2921161849445362
2404	110-114	1.1115891675082636
2404	115-119	1.1393916100679249
2404	120-124	1.3670368131098698
2404	125-129	1.1705322711934336
2404	130-134	1.3903062048577972
2404	135-139	1.5385175061938767
2404	140-144	1.5631207078651386
2404	145-149	1.6221461646317792
2404	150-154	1.5444407183373663
2404	155-159	1.679014649252771
2404	160-164	1.91020558391833
2404	165-169	1.710544007102019
2404	170-174	1.9718697023084708
2404	175-179	1.8032841719007955
2404	180-184	1.798955264744425
2404	185-189	2.0988396052435583
2404	190-194	2.129076413275726
2404	195-199	1.9549271996808528
2404	200-204	2.4151717856778347
2404	205-209	2.523272067682317
2404	210-214	3.1671409053744703
2404	215-219	2.7999618555751695
2404	220-224	2.615072999514503
2404	225-229	2.8821773341248615
2404	230-234	2.559782800355194
2404	235-239	2.9010533897833177
2404	240-244	3.267994397149849
2404	245-249	3.6688855860834586
2404	250-254	3.57049542479821
2404	255-259	3.7905228564788693
2404	260-264	3.9364981526298948
2404	265-269	4.168599371508996
2404	270-274	4.180114011766602
2404	275-279	4.435181031001967
2404	280-284	4.156027496110077
2404	285-289	4.422153353610991
2404	290-294	4.720618815083743
2404	295-299	4.723407238621014
2404	300-301	2.888111411912508
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	442.0
17	974.0
18	1012.0
19	966.0
20	998.0
21	1183.0
22	1389.0
23	1669.0
24	2169.0
25	3171.0
26	4551.0
27	7083.0
28	10683.0
29	15019.0
30	19084.0
31	21153.0
32	28552.0
33	40731.0
34	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	99.4461263030951	0.17281356648660073	0.055946838071201675	0.3251132923470942
2	0.49792685883369486	98.74492593260271	0.1112720446082789	0.6458751639553171
3	0.4736832290028408	0.4152498647951413	0.1833813025667166	98.9276856036353
4	0.2088681954658196	0.2660582966052702	99.16825700734147	0.3568165005874418
5	0.2809774534242573	98.94260476045429	0.42706086394350606	0.3493569221779483
6	0.26854482274176805	0.6421453747505703	0.17405682955484966	98.91525297295281
7	0.4152498647951413	31.5173404116444	37.642275917372736	30.42513380618772
8	34.273033002418146	33.32504491287834	0.471196702866343	31.93072538183717
9	99.27828578888149	0.1734351980207252	0.2878154002996264	0.26046361279815006
10-14	39.960961539656985	19.943680183008325	39.87293851442496	0.22241976290973287
15-19	0.27166817938803167	39.85129741759813	39.73392681744147	20.143107585572366
20-24	19.850528187623187	0.4661978574048858	39.74582330740582	39.93745064756611
25-29	20.122199947269714	0.36513234803977657	42.26369619396785	37.248971510722654
30-34	0.42821305931677445	37.29943448016825	0.5688778778143846	61.70347458270059
35-39	19.339968804495737	1.1470691400837352	56.79683865655342	22.71612339886711
40-44	39.813910553155175	18.82665863820544	20.443962294431376	20.915468514208012
45-49	0.8963811287135612	20.009556089767518	20.033446314186314	59.06061646733261
50-54	38.65493932244223	1.609149916873239	20.221331222809585	39.514579537874944
55-59	37.94751987732566	21.588617772843367	39.59396486057244	0.8698974892585272
60-64	21.143896498670106	35.31615370719887	3.2490547468216446	40.29089504730938
65-69	36.830225968610684	39.901158227934964	1.8311055880162148	21.43751021543813
70-74	40.84638234166697	18.506138787924947	0.7762604399350224	39.87121843047306
75-79	0.7517388643997952	0.2667299359398609	39.72727613291542	59.25425506674492
80-84	19.952419012413518	19.910332942028624	0.6031920473561955	59.534055998201666
85-89	59.56801071105626	0.30437085033996614	39.54810233455065	0.5795161040531157
90-94	0.4168113494595317	0.2889075970332325	76.28109847339877	23.013182580108467
95-99	1.2997964851524113	38.729412705373214	19.759680073160403	40.21111073631397
100-104	0.26410486200427213	20.44094137821991	59.04487282844683	20.250080931328988
105-109	20.197328859345678	39.24336434203502	0.7416084602373098	39.81769833838199
110-114	0.3118353649311499	20.105003744775132	41.57700670558565	38.006154184708066
115-119	20.045623039635014	0.7621348781134473	19.790494199570784	59.40174788268075
120-124	0.5074878538154781	56.43935413820882	2.438493522868851	40.61466448510685
125-129	57.498345433458894	24.48295577521916	17.435565736648055	0.5831330546738847
130-134	2.4180813017896603	17.955667675243323	39.4163269289967	40.209924093970315
135-139	1.7164201524149052	2.4744890295316395	38.58039836168998	57.228692456363476
140-144	0.8795102286100609	0.7058617322282595	56.40859353481734	42.00603450434434
145-149	19.803115932682232	21.142308284734284	19.294563460110375	39.76001232247311
150-154	0.7727758423933127	38.64192380973856	20.51545113248176	40.06984921538637
155-159	21.241523788521967	70.16335971212204	4.407379378436217	4.187737120919766
160-164	21.108628444178215	37.42589072116114	20.651096460173665	20.814384374486984
165-169	18.17908360092347	4.12953618545563	40.585464654288586	37.10591555933232
170-174	21.92369895816356	38.603863946942816	18.67657936637288	20.79585772852074
175-179	18.960611278249235	2.5853668936094434	39.39536826053315	39.05865356760817
180-184	42.17149006784495	1.4960006723034798	38.007222245217676	18.32528701463389
185-189	22.55837469200544	37.2152775338355	19.60677061117161	20.61957716298746
190-194	55.05630526059535	19.450976652202392	1.7534062988608445	23.73931178834141
195-199	18.876617427427554	1.8592100804477967	60.15838552190665	19.105786970218
200-204	0.7995781120514303	38.10326209788805	19.937972426609075	41.159187363451444
205-209	19.33014786903495	38.67713187596038	2.2454884804380413	39.74723177456663
210-214	3.3607212580511274	20.171885488242918	71.87818650433749	4.589206749368464
215-219	2.618737452879466	4.3798339846822865	35.268500512897965	57.73292804954028
220-224	36.70798256371773	2.474546905966462	20.920139107485966	39.89733142282984
225-229	21.072939848127042	1.8691765441215387	18.905108385272406	58.152775222479015
230-234	16.95553291915443	1.263968452477005	59.1782061884783	22.60229243989027
235-239	18.201403639776185	21.321373972623476	0.4968491263830796	59.98037326121726
240-244	2.751105264619665	56.361678436798556	20.424860942435345	20.462355356146432
245-249	1.5021543257674035	37.87065961147609	21.087002929276725	39.54018313347978
250-254	38.441073240892756	40.35459567675373	2.660478683941452	18.543852398412056
255-259	20.424649772079917	19.536874484586935	20.41250752566314	39.62596821767001
260-264	20.533378226654733	18.42026984092828	19.77137316602976	41.27497876638722
265-269	1.4031215113619515	20.36078457212587	19.93945530044895	58.29663861606322
270-274	18.638369218789038	20.338727248976195	59.476639259538274	1.5462642726964886
275-279	21.409230276608422	18.976692414329406	0.9911092418395495	58.62296806722262
280-284	58.32130758678262	0.509026309024782	19.839300394240876	21.330365709951717
285-289	41.140906153899095	1.6368935726056097	19.840261413585807	37.38193885990949
290-294	21.0274821449105	37.23466506535155	19.805670188708174	21.93218260102978
295-299	37.235524418941104	19.433882064951593	40.73839354529788	2.592199970809424
300-301	9.772124096275281	1.4258259357554681	0.5527592202800403	88.24929074768922
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.3333333333333333
3	0.8333333333333333
4	0.0
5	0.0
6	0.0
7	0.0
8	0.25
9	0.0
10	0.0
11	0.25
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	1.0
19	1.5
20	1.5
21	1.0
22	1.5
23	2.25
24	3.75
25	5.0
26	5.5
27	6.5
28	5.75
29	5.5
30	7.666666666666666
31	8.333333333333332
32	10.583333333333334
33	9.666666666666666
34	10.5
35	12.583333333333334
36	9.833333333333334
37	8.333333333333332
38	10.916666666666668
39	13.5
40	15.833333333333336
41	19.749999999999993
42	27.24999999999999
43	37.16666666666667
44	37.58333333333333
45	45.41666666666667
46	53.50000000000001
47	84.41666666666666
48	190.91666666666674
49	361.8333333333333
50	513.3333333333329
51	1103.8333333333396
52	10076.49999999996
53	29491.24999999996
54	8494.166666666646
55	2764.250000000001
56	781.4166666666665
57	286.5833333333333
58	69.33333333333334
59	33.66666666666666
60	24.083333333333332
61	11.833333333333332
62	7.166666666666666
63	9.5
64	7.416666666666666
65	1.5833333333333333
66	0.3333333333333333
67	0.3333333333333333
68	0.0
69	0.25
70	0.5
71	1.0
72	0.8333333333333333
73	0.6666666666666666
74	0.8333333333333333
75	0.8333333333333333
76	0.0
77	0.0
78	0.0
79	0.25
80	0.3333333333333333
81	0.0
82	0.0
83	0.3333333333333333
84	0.25
85	0.3333333333333333
86	0.3333333333333333
87	0.0
88	0.8333333333333333
89	0.5
90	0.3333333333333333
91	1.4999999999999998
92	0.8333333333333333
93	0.75
94	1.9166666666666665
95	2.9166666666666665
96	6.833333333333333
97	11.583333333333336
98	18.75
99	30.666666666666664
100	101.33333333333323
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	2.487435342227573E-4
35-39	1.2438383358438136E-4
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	2.5053457815614065E-4
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	2.50911435790509E-4
190-194	0.0
195-199	0.0
200-204	0.0
205-209	1.256426622172412E-4
210-214	1.8230935240941128
215-219	2.5172843033478625E-4
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	1.2682597700391387E-4
275-279	0.0
280-284	0.0
285-289	0.0
290-294	1.2776434122789196E-4
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
10-19	28.0
20-29	25.0
30-39	30.0
40-49	58.0
50-59	184.0
60-69	351.0
70-79	38.0
80-89	24.0
90-99	104.0
100-109	59.0
110-119	64.0
120-129	50.0
130-139	94.0
140-149	83.0
150-159	69.0
160-169	61.0
170-179	86.0
180-189	65.0
190-199	80.0
200-209	190.0
210-219	254.0
220-229	207.0
230-239	191.0
240-249	192.0
250-259	211.0
260-269	314.0
270-279	463.0
280-289	594.0
290-299	941.0
300-302	155757.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.185855396072532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.00753693096172	4.639857770704992
2	6.893779519646266	0.8528784648187633
3	2.7434428700633102	0.5091162264479352
4	1.7787157069641242	0.4401151261601199
5	1.3164506079790976	0.4071686548515233
6	1.0350718520751683	0.3841682880889182
7	0.7436438548889559	0.32200513467647185
8	0.964727163099186	0.47741301820758764
9	0.7838408200180885	0.43638533695537307
>10	7.486684755300975	9.272877594534616
>50	0.6130037182192744	2.496472241043844
>100	0.4522158577027435	5.385815611654348
>500	0.09044317154054868	3.422081595355169
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.09044317154054868	70.95364493650034
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	14627	9.092604449638522	No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	14460	8.988791983439736	No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	14392	8.946521039119272	No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	12463	7.747393809793183	No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	12105	7.524849720576625	No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	12098	7.520498299837754	No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	11767	7.314738262042557	No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	11262	7.000814337309704	No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	10967	6.817433034742987	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	728	0.45254775684260906	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	682	0.42395270627288384	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	649	0.40343886564677656	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	605	0.3760870781453002	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	603	0.3748438150770512	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	585	0.3636544474628109	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	577	0.3586813951898152	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	562	0.3493569221779483	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	514	0.31951860853997405	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	435	0.27040971734414143	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	390	0.24243629830854058	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	379	0.2355983514331715	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	343	0.21321961620469082	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	334	0.20762493239757066	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	327	0.20327351165869945	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	304	0.18897598637383678	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	299	0.18586782870321444	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	284	0.1765433556913475	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	278	0.17281356648660073	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	262	0.1628674619406093	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	248	0.15416462046286683	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	243	0.15105646279224452	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	230	0.1429752528486265	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	224	0.13924546364387974	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	224	0.13924546364387974	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	214	0.1330291483026351	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	213	0.13240751676851065	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	204	0.12681283296139045	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	169	0.10505572926703426	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	4.973052272995704E-4	0.0	0.0	0.0
175-179	0.0	6.216315341244631E-4	0.0	0.0	0.0
180-184	0.0	6.216315341244631E-4	0.0	0.0	0.0
185-189	0.0	6.216315341244631E-4	0.0	0.0	0.0
190-194	0.0	6.216315341244631E-4	0.0	0.0	0.0
195-199	0.0	6.216315341244631E-4	0.0	0.0	0.0
200-204	0.0	6.216315341244631E-4	0.0	1.243263068248926E-4	0.0
205-209	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
210-214	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
215-219	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
220-224	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
225-229	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
230-234	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
235-239	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
240-244	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
245-249	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
250-254	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
255-259	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
260-264	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
265-269	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
270-274	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
275-279	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
280-284	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
285-289	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
290	0.0	6.216315341244631E-4	0.0	6.216315341244631E-4	0.0
>>END_MODULE
