##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	JAMS2_cleaned_R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	146335
Sequences flagged as poor quality	0
Sequence length	81-301
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.40528239997266	40.0	40.0	40.0	40.0	40.0
2	38.860149656609835	40.0	40.0	40.0	40.0	40.0
3	39.28503775583422	40.0	40.0	40.0	40.0	40.0
4	39.55890251819455	40.0	40.0	40.0	40.0	40.0
5	38.70210134280931	40.0	40.0	40.0	40.0	40.0
6	38.99411624013394	40.0	40.0	40.0	40.0	40.0
7	38.738620289062766	40.0	40.0	40.0	40.0	40.0
8	39.01199302969214	40.0	40.0	40.0	40.0	40.0
9	39.41392011480507	40.0	40.0	40.0	40.0	40.0
10-14	39.25084224553251	40.0	40.0	40.0	40.0	40.0
15-19	39.24422728670516	40.0	40.0	40.0	40.0	40.0
20-24	39.382704069429735	40.0	40.0	40.0	40.0	40.0
25-29	39.40969419482694	40.0	40.0	40.0	40.0	40.0
30-34	38.73155704376943	40.0	40.0	40.0	36.8	40.0
35-39	39.443326613592106	40.0	40.0	40.0	40.0	40.0
40-44	39.340740082686985	40.0	40.0	40.0	40.0	40.0
45-49	39.15537362900195	40.0	40.0	40.0	40.0	40.0
50-54	39.397694331499636	40.0	40.0	40.0	40.0	40.0
55-59	39.19865514060204	40.0	40.0	40.0	40.0	40.0
60-64	38.824844363959414	40.0	40.0	40.0	36.8	40.0
65-69	39.11881094748352	40.0	40.0	40.0	40.0	40.0
70-74	38.557758567670064	40.0	40.0	40.0	33.6	40.0
75-79	38.964009977107324	40.0	40.0	40.0	40.0	40.0
80-84	38.80742089362509	40.0	40.0	40.0	40.0	40.0
85-89	39.207059040243124	40.0	40.0	40.0	40.0	40.0
90-94	39.17874163722469	40.0	40.0	40.0	40.0	40.0
95-99	38.78484825705753	40.0	40.0	40.0	36.8	40.0
100-104	39.26613955840446	40.0	40.0	40.0	40.0	40.0
105-109	39.099227105300926	40.0	40.0	40.0	40.0	40.0
110-114	39.32123034448826	40.0	40.0	40.0	40.0	40.0
115-119	39.31111642623332	40.0	40.0	40.0	40.0	40.0
120-124	38.69680946323641	40.0	40.0	40.0	36.8	40.0
125-129	39.147128447639616	40.0	40.0	40.0	40.0	40.0
130-134	39.000895559650196	40.0	40.0	40.0	40.0	40.0
135-139	38.94569634180512	40.0	40.0	40.0	36.8	40.0
140-144	39.001280675737554	40.0	40.0	40.0	40.0	40.0
145-149	38.90556212370753	40.0	40.0	40.0	40.0	40.0
150-154	38.70726637826592	40.0	40.0	40.0	36.8	40.0
155-159	39.118328837025935	40.0	40.0	40.0	40.0	40.0
160-164	38.78557440223668	40.0	40.0	40.0	36.8	40.0
165-169	39.075657016063694	40.0	40.0	40.0	40.0	40.0
170-174	39.22817348040623	40.0	40.0	40.0	40.0	40.0
175-179	39.23091811210891	40.0	40.0	40.0	40.0	40.0
180-184	38.77760555487213	40.0	40.0	40.0	33.6	40.0
185-189	39.275572375063895	40.0	40.0	40.0	40.0	40.0
190-194	38.68725336759591	40.0	40.0	40.0	36.8	40.0
195-199	39.34291726155033	40.0	40.0	40.0	40.0	40.0
200-204	38.877895556968	40.0	40.0	40.0	40.0	40.0
205-209	39.07552676002433	40.0	40.0	40.0	40.0	40.0
210-214	38.88145918931751	40.0	40.0	40.0	40.0	40.0
215-219	38.95206132131365	40.0	40.0	40.0	40.0	40.0
220-224	39.005980411589015	40.0	40.0	40.0	40.0	40.0
225-229	38.772472336324675	40.0	40.0	40.0	40.0	40.0
230-234	39.128366288249005	40.0	40.0	40.0	40.0	40.0
235-239	38.71837387482828	40.0	40.0	40.0	40.0	40.0
240-244	38.80016676804888	40.0	40.0	40.0	36.8	40.0
245-249	37.80922281988367	40.0	40.0	40.0	34.4	40.0
250-254	38.88932479444471	40.0	40.0	40.0	40.0	40.0
255-259	38.70945588506674	40.0	40.0	40.0	40.0	40.0
260-264	38.25480790917976	40.0	40.0	40.0	33.6	40.0
265-269	37.45259583873711	40.0	36.8	40.0	30.4	40.0
270-274	38.79107400267889	40.0	40.0	40.0	40.0	40.0
275-279	37.94949046196748	40.0	40.0	40.0	33.6	40.0
280-284	38.51328899794271	40.0	40.0	40.0	36.8	40.0
285-289	38.563531158537685	40.0	40.0	40.0	36.8	40.0
290-294	38.17824686741862	40.0	40.0	40.0	33.6	40.0
295-299	38.51285014838464	40.0	40.0	40.0	36.8	40.0
300-301	32.78517303515808	32.0	26.0	40.0	18.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.27508710023117544
1101	2	0.2843514664273954
1101	3	0.3643285897059272
1101	4	-0.033035100374476656
1101	5	0.36992465632931726
1101	6	-0.2150786241259226
1101	7	-0.0888502900779713
1101	8	0.0346118893072358
1101	9	0.03914628978349555
1101	10-14	0.1481206486770219
1101	15-19	0.11277700787940859
1101	20-24	0.029352989248089045
1101	25-29	-0.08313789081486789
1101	30-34	0.028499198329214437
1101	35-39	0.002329302831519442
1101	40-44	2.9582881325040944E-5
1101	45-49	-0.13918185993500032
1101	50-54	-0.13682595063674796
1101	55-59	-0.33054948809653695
1101	60-64	-0.1455277970553368
1101	65-69	-0.11329474043153454
1101	70-74	-0.23943325787348613
1101	75-79	-0.1597024966369247
1101	80-84	-0.07888075465573507
1101	85-89	-0.16729913336417468
1101	90-94	-0.06548498556415439
1101	95-99	-0.06721485291174645
1101	100-104	-0.121892642048266
1101	105-109	-0.14883086589838257
1101	110-114	-0.07885973372094668
1101	115-119	-0.1528186918529002
1101	120-124	-0.14294203818121076
1101	125-129	-0.17153304920013568
1101	130-134	-0.26796104927213094
1101	135-139	-0.24381133418322776
1101	140-144	-0.17318099203232862
1101	145-149	-0.0524705124305882
1101	150-154	-0.03515623782750055
1101	155-159	-0.1781184277583634
1101	160-164	-0.05198742966368286
1101	165-169	-0.11833515238244274
1101	170-174	-0.006827890238199075
1101	175-179	-0.047429816972865524
1101	180-184	-0.0034474644526127918
1101	185-189	-0.14808493100429843
1101	190-194	0.08464500057618096
1101	195-199	0.1478196121410278
1101	200-204	0.02956271074232575
1101	205-209	0.05659099764650222
1101	210-214	0.058782847629942125
1101	215-219	-0.272045243969508
1101	220-224	0.02490456504251881
1101	225-229	-0.23572492075853546
1101	230-234	-0.1434420980826161
1101	235-239	0.010935735831665738
1101	240-244	-0.21889414676662966
1101	245-249	0.7439457979665818
1101	250-254	-0.1951007289785167
1101	255-259	-0.010014905570820076
1101	260-264	-0.3199107860562407
1101	265-269	-0.24659488434945587
1101	270-274	-0.1664964328807983
1101	275-279	-0.48214519793554445
1101	280-284	-0.2970103129260977
1101	285-289	-0.38359622881305455
1101	290-294	-0.5576007828394793
1101	295-299	-0.30745106135755407
1101	300-301	-0.6791170773592725
1102	1	-0.3473955888440159
1102	2	-0.05410609050841941
1102	3	-0.23753476433133613
1102	4	-0.20260031776577847
1102	5	-0.3440504989501889
1102	6	-0.005761853939588946
1102	7	-0.34708010374257015
1102	8	-0.2545185454753778
1102	9	-0.24760319261816477
1102	10-14	-0.2921381504948215
1102	15-19	-0.20765777472928448
1102	20-24	-0.2691045676877266
1102	25-29	-0.22031180385833693
1102	30-34	-0.24567264432089075
1102	35-39	-0.33757752946662123
1102	40-44	-0.3256847028329659
1102	45-49	-0.486821611487791
1102	50-54	-0.21124624049182472
1102	55-59	-0.3457254715333846
1102	60-64	-0.3668424968482995
1102	65-69	-0.31761151061787274
1102	70-74	-0.41476452081344206
1102	75-79	-0.2535741322476852
1102	80-84	-0.48487454347561254
1102	85-89	-0.30313764268095156
1102	90-94	-0.26931521330742925
1102	95-99	-0.35665584670057626
1102	100-104	-0.43028808718283784
1102	105-109	-0.22506274995635778
1102	110-114	-0.42332143144351164
1102	115-119	-0.33779798791915283
1102	120-124	-0.48000207958907737
1102	125-129	-0.36094298708833605
1102	130-134	-0.5138823743238845
1102	135-139	-0.34500181037370936
1102	140-144	-0.44869859037600435
1102	145-149	-0.4660626449357679
1102	150-154	-0.2615744572891927
1102	155-159	-0.22786998055339325
1102	160-164	-0.3581261460197851
1102	165-169	-0.3946291482416484
1102	170-174	-0.45977820079721
1102	175-179	-0.37562857473684375
1102	180-184	-0.24736050793086406
1102	185-189	-0.4650828606109272
1102	190-194	-0.5712245646412129
1102	195-199	-0.5199177591077273
1102	200-204	-0.4552257330912255
1102	205-209	-0.6217234687409388
1102	210-214	-0.21214390193889443
1102	215-219	-0.5115054721017955
1102	220-224	-0.4122069120253897
1102	225-229	-0.8202311168105254
1102	230-234	-0.3337453955838754
1102	235-239	-0.5287358539624591
1102	240-244	-0.3789895836887851
1102	245-249	0.1645468603939193
1102	250-254	-0.6816319339993271
1102	255-259	-0.6000260139444578
1102	260-264	-0.6289915573318083
1102	265-269	-0.7071056966438718
1102	270-274	-0.6183003549579453
1102	275-279	-0.8951277314227539
1102	280-284	-0.9727452161276418
1102	285-289	-0.9127732150291621
1102	290-294	-0.8324003350466853
1102	295-299	-0.7279871617814706
1102	300-301	-1.8733813730356985
1103	1	-0.3785811558543273
1103	2	0.05680456241253751
1103	3	-0.2777409498983445
1103	4	-0.18782368546337125
1103	5	0.07673987905668156
1103	6	-0.23497147593271706
1103	7	-0.3473378357013317
1103	8	-0.20073847674685652
1103	9	-0.1895275912435892
1103	10-14	-0.2519835113195654
1103	15-19	-0.2294790805712097
1103	20-24	-0.14156161236126508
1103	25-29	-0.16637709595455163
1103	30-34	-0.09190975772295218
1103	35-39	-0.19841945386524174
1103	40-44	-0.27276374063364983
1103	45-49	-0.26719961836065664
1103	50-54	-0.28236307897979884
1103	55-59	-0.36232340967772103
1103	60-64	-0.3497462769170241
1103	65-69	-0.35841907075532475
1103	70-74	-0.5806579916038146
1103	75-79	-0.2786600429006114
1103	80-84	-0.47630066031410223
1103	85-89	-0.5345809416500202
1103	90-94	-0.3340231170874972
1103	95-99	-0.5585802453260058
1103	100-104	-0.1630887744680578
1103	105-109	-0.25193560219003075
1103	110-114	-0.33158603625451377
1103	115-119	-0.35736843465455337
1103	120-124	-0.3879058596578133
1103	125-129	-0.3110288977412594
1103	130-134	-0.5466658794785246
1103	135-139	-0.6476994048754321
1103	140-144	-0.5445233326440544
1103	145-149	-0.5163547411557019
1103	150-154	-0.5675538387325005
1103	155-159	-0.5846053757423917
1103	160-164	-0.6769405790094822
1103	165-169	-0.4011927221248044
1103	170-174	-0.49037957536765475
1103	175-179	-0.28339489776089266
1103	180-184	-0.4704189271748547
1103	185-189	-0.45631997401299174
1103	190-194	-0.6773929495209359
1103	195-199	-0.34997296432478464
1103	200-204	-0.3619695937976033
1103	205-209	-0.4067980442095944
1103	210-214	-0.24536775747986894
1103	215-219	-0.6692602447165612
1103	220-224	-0.3852228601992067
1103	225-229	-0.9095615517802855
1103	230-234	-0.5863747996258084
1103	235-239	-0.6817419017854647
1103	240-244	-0.4903521732762073
1103	245-249	0.1014015619844244
1103	250-254	-0.4818549071177216
1103	255-259	-0.7702052963842476
1103	260-264	-0.6810966732304777
1103	265-269	-0.8851673801339643
1103	270-274	-0.5586468672365257
1103	275-279	-0.6782084623269142
1103	280-284	-0.6478587473245057
1103	285-289	-0.8579510985063408
1103	290-294	-0.8664368802546889
1103	295-299	-0.9348957263593718
1103	300-301	-3.6797394839982758
1104	1	0.0669349550890388
1104	2	-0.30999869859768125
1104	3	-0.0069908312769442205
1104	4	-0.0462544321311924
1104	5	-0.3508147946266149
1104	6	-0.5035303337164336
1104	7	-0.37040646357520046
1104	8	-0.17592719261624268
1104	9	0.008324282974584207
1104	10-14	-0.17923020070401918
1104	15-19	-0.2165420146177155
1104	20-24	-0.2090697001842372
1104	25-29	-0.19835225016238667
1104	30-34	-0.4459864518522636
1104	35-39	-0.21564586977345357
1104	40-44	-0.24272793853728558
1104	45-49	-0.380140997819737
1104	50-54	-0.20280133114732735
1104	55-59	-0.2817505761358916
1104	60-64	-0.5421493308789778
1104	65-69	-0.29067287742400794
1104	70-74	-0.703585999195596
1104	75-79	-0.3073258756228654
1104	80-84	-0.4465133161394874
1104	85-89	-0.19548073891525775
1104	90-94	-0.29393167076907645
1104	95-99	-0.5672555677605402
1104	100-104	-0.3044233730963626
1104	105-109	-0.29817293126736644
1104	110-114	-0.2620592278172964
1104	115-119	-0.26993885263323847
1104	120-124	-0.3065013822390057
1104	125-129	-0.5298202534760605
1104	130-134	-0.6522087341565168
1104	135-139	-0.5849948368730082
1104	140-144	-0.5216065401668004
1104	145-149	-0.4523457690640811
1104	150-154	-0.6311142062431685
1104	155-159	-0.5503664938030468
1104	160-164	-0.3512433008315057
1104	165-169	-0.4334715471258974
1104	170-174	-0.49991069731045457
1104	175-179	-0.32168157334213987
1104	180-184	-0.6599267561874953
1104	185-189	-0.3126560823682496
1104	190-194	-0.866880073706767
1104	195-199	-0.3375914778907614
1104	200-204	-0.5555148640387202
1104	205-209	-0.5261303135448756
1104	210-214	-0.2801656697431625
1104	215-219	-0.43099254855989244
1104	220-224	-0.42681195565708663
1104	225-229	-0.8879698682190593
1104	230-234	-0.5772163645656292
1104	235-239	-0.6596256959449676
1104	240-244	-0.469222897513788
1104	245-249	0.13868405346525492
1104	250-254	-0.5547462499120144
1104	255-259	-0.7193645941455458
1104	260-264	-0.6104172961006853
1104	265-269	-1.157328877783101
1104	270-274	-0.7993784377839077
1104	275-279	-1.0333445534281438
1104	280-284	-0.9442828511937194
1104	285-289	-0.8723927152082283
1104	290-294	-0.8629067072831234
1104	295-299	-0.6705307607421744
1104	300-301	-2.1751966382158727
1201	1	0.10260441115598695
1201	2	0.4875605761582449
1201	3	0.24579856900199815
1201	4	0.03906634890088867
1201	5	-0.03571716561685179
1201	6	0.3359048127270796
1201	7	-0.013746770409234443
1201	8	-0.029518545475376357
1201	9	0.07739680738183807
1201	10-14	-0.2121381504948232
1201	15-19	-0.06599110806261876
1201	20-24	-0.010771234354393755
1201	25-29	-0.05031180385834233
1201	30-34	0.024327355679112372
1201	35-39	-0.0575775294666272
1201	40-44	-0.09401803616628968
1201	45-49	-0.1368216114877967
1201	50-54	-0.21791290715849243
1201	55-59	-0.16739213820005716
1201	60-64	-0.40350916351496835
1201	65-69	-0.14761151061786393
1201	70-74	-0.2580978541467758
1201	75-79	-0.13024079891435036
1201	80-84	-0.31820787680894114
1201	85-89	-0.26813764268095497
1201	90-94	-0.21264854664075727
1201	95-99	-0.08332251336724283
1201	100-104	-0.13528808718284324
1201	105-109	-0.29172941662302065
1201	110-114	-0.19498809811017992
1201	115-119	-0.25779798791915454
1201	120-124	-0.26833541292241136
1201	125-129	-0.17427632042166863
1201	130-134	-0.2288823743238808
1201	135-139	-0.31000181037371277
1201	140-144	-0.1453652570426769
1201	145-149	-0.1527293116024353
1201	150-154	-0.3349077906225304
1201	155-159	-0.1495366472200601
1201	160-164	-0.2564594793531185
1201	165-169	-0.21629581490832095
1201	170-174	-0.19977820079721198
1201	175-179	-0.22896190807017547
1201	180-184	-0.2606938412641995
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2301	70-74	0.6765475359241506
2301	75-79	0.4092627471849326
2301	80-84	0.6991325487229645
2301	85-89	0.48430916582968564
2301	90-94	0.3306138647067556
2301	95-99	0.6064647206753193
2301	100-104	0.29655588444836667
2301	105-109	0.36316420039825204
2301	110-114	0.4018204125281244
2301	115-119	0.3408899553432647
2301	120-124	0.5554589133187307
2301	125-129	0.4143407008549289
2301	130-134	0.48313890227186107
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2301	140-144	0.36864183515590554
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2301	150-154	0.6388510746256983
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2301	245-249	1.4461175084639137
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2301	295-299	0.490703816427434
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2302	1	0.24298902654059873
2302	2	-0.22910609050842368
2302	3	-0.021295448092018887
2302	4	0.14013472496926482
2302	5	0.033299928400239764
2302	6	-0.21003535821309072
2302	7	0.08262075095828436
2302	8	0.21001136905454132
2302	9	0.25239680738183523
2302	10-14	0.08183620847953677
2302	15-19	0.16866701159550246
2302	20-24	0.1671774835943225
2302	25-29	0.12109845255191942
2302	30-34	0.2754812018329531
2302	35-39	0.12075580386671447
2302	40-44	0.13140931426105595
2302	45-49	0.28830659364040656
2302	50-54	0.011446067200481025
2302	55-59	0.2565822207743125
2302	60-64	0.44260194759614535
2302	65-69	0.2252517372453795
2302	70-74	0.38963718858826724
2302	75-79	0.24779338911983473
2302	80-84	0.3690143454132837
2302	85-89	0.18737517783186775
2302	90-94	0.3029070089147936
2302	95-99	0.6741133840686544
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2302	105-109	0.3183560534624519
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2302	115-119	0.2660909009697363
2302	120-124	0.3627329631459659
2302	125-129	0.25200573086038247
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2302	145-149	0.3564587225856002
2302	150-154	0.38834007262533277
2302	155-159	0.3342667715833585
2302	160-164	0.30969436680072704
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2302	170-174	0.3387260727070682
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2302	295-299	0.2593486627264028
2302	300-301	2.465334589817232
2303	1	0.201717146020286
2303	2	0.06873663739693825
2303	3	0.01945897262064733
2303	4	0.10505451869907745
2303	5	0.1536182553991523
2303	6	0.242741973478644
2303	7	0.3427424432302928
2303	8	0.18553016712031223
2303	9	0.2349994517798919
2303	10-14	0.03309149459912675
2303	15-19	0.11108265672513085
2303	20-24	0.07984811150503646
2303	25-29	0.0336513137477823
2303	30-34	0.01847140578349382
2303	35-39	0.10655956169551928
2303	40-44	0.14159435075089277
2303	45-49	0.26369335023802165
2303	50-54	0.18461666834603108
2303	55-59	0.25487995644156314
2303	60-64	0.28806703690257507
2303	65-69	0.21441006210307734
2303	70-74	0.3313141152338801
2303	75-79	0.20979399579684355
2303	80-84	0.3267503695376135
2303	85-89	0.11383521744431135
2303	90-94	0.2495087254538788
2303	95-99	0.3065313488457093
2303	100-104	0.15526862819642417
2303	105-109	0.15014949778198883
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2303	120-124	0.3965462850595003
2303	125-129	0.23788443114409574
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2303	135-139	0.42711022859636927
2303	140-144	0.3082429545091685
2303	145-149	0.3908858588916573
2303	150-154	0.374918235821454
2303	155-159	0.32270065271035264
2303	160-164	0.37182166191340826
2303	165-169	0.35759771327540335
2303	170-174	0.3527652925917977
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2303	300-301	0.6712235935002724
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2401	2	0.19666421761483122
2401	3	0.07422994155101748
2401	4	-0.08187202644925407
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2401	7	0.18590308953474022
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2401	9	0.03951165332021134
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2401	15-19	0.11063354179731988
2401	20-24	-0.022479917827787688
2401	25-29	0.03387587121168423
2401	30-34	0.22932735567911067
2401	35-39	0.038402862690240624
2401	40-44	-0.03134296613828269
2401	45-49	0.08037726806402645
2401	50-54	-0.1337812544974284
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2401	70-74	0.22197217386442958
2401	75-79	0.11578161004922549
2401	80-84	0.15855682907341162
2401	85-89	-0.04154100402548977
2401	90-94	-0.038082720310228524
2401	95-99	0.1817195034394814
2401	100-104	0.03930575035217032
2401	105-109	0.06675797833496944
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2401	115-119	0.09092750227692648
2401	120-124	0.21299511929047554
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2401	135-139	0.023415556573070262
2401	140-144	0.10907451886768627
2401	145-149	0.19005780324350496
2401	150-154	0.22313142506374106
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2401	245-249	1.2412542865794194
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2401	255-259	0.332890952424556
2401	260-264	0.5042762800745564
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2401	295-299	0.563549829616683
2401	300-301	2.6801080688395587
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2402	2	-0.05189090063500146
2402	3	0.13081966604841
2402	4	0.17756845860552772
2402	5	0.14181447995276386
2402	6	0.06639004479458777
2402	7	0.502181499632961
2402	8	0.548646011486646
2402	9	0.2988103094915431
2402	10-14	0.21990826300728372
2402	15-19	0.22284855438464035
2402	20-24	0.2028152635358964
2402	25-29	0.1735489556353329
2402	30-34	0.3736311531474641
2402	35-39	0.12581909500595856
2402	40-44	0.17507479083792532
2402	45-49	0.26630075138140086
2402	50-54	0.26831072153348856
2402	55-59	0.10773444407842447
2402	60-64	0.33824189133735416
2402	65-69	0.28709313073234455
2402	70-74	0.5342439180051244
2402	75-79	0.20659464412361928
2402	80-84	0.3164123763556077
2402	85-89	0.2301534965595522
2402	90-94	0.2216130567347676
2402	95-99	0.4121205246074524
2402	100-104	0.21040811534881243
2402	105-109	0.2170469546849958
2402	110-114	0.30589797783918016
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2402	120-124	0.30143251956703665
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2402	135-139	0.4124876411030911
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2403	1	-0.18923769410717028
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2403	5	0.3884056414006878
2403	6	0.12722060220076514
2403	7	0.42791989625743554
2403	8	0.2884639106649729
2403	9	0.1309932986099085
2403	10-14	0.19738816529465453
2403	15-19	0.2339913480777227
2403	20-24	0.18003578318946722
2403	25-29	0.12714433649254175
2403	30-34	0.2979589346264859
2403	35-39	0.14180843544566812
2403	40-44	-0.029404001078574993
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2403	50-54	0.18642042617484123
2403	55-59	0.2556429495192418
2403	60-64	0.2809645206955551
2403	65-69	0.15352884025931957
2403	70-74	0.43504249673041784
2403	75-79	0.3530925344189768
2403	80-84	0.22047633371736453
2403	85-89	0.10665183100325493
2403	90-94	0.16222864634168843
2403	95-99	0.13583538136960271
2403	100-104	0.12215050930839055
2403	105-109	0.19816532021908273
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2403	125-129	0.13040789010464948
2403	130-134	0.29432815199190543
2403	135-139	0.2015420492754103
2403	140-144	0.1779856201503094
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2403	150-154	0.35400449007922674
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2403	160-164	0.3194001697696862
2403	165-169	0.15944102719694797
2403	170-174	0.22830951850104242
2403	175-179	0.19714335508771796
2403	180-184	0.4907096675077369
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2403	300-301	2.762675233756781
2404	1	0.1888947337366318
2404	2	-0.04846092921810197
2404	3	-0.008502506266822252
2404	4	0.1296577467503468
2404	5	0.37799251180249627
2404	6	0.23832416756579278
2404	7	0.48813495002087137
2404	8	0.007309411513872988
2404	9	0.2588484202850623
2404	10-14	0.34092636563421763
2404	15-19	0.2133099672062002
2404	20-24	0.18514274414022935
2404	25-29	0.20619357248574488
2404	30-34	0.09900477503395422
2404	35-39	0.18011064257639475
2404	40-44	0.21065938318854194
2404	45-49	0.32446871109284814
2404	50-54	0.24697956595978354
2404	55-59	0.25846807685370266
2404	60-64	0.36417900852804763
2404	65-69	0.1988938657262196
2404	70-74	0.45528924262742265
2404	75-79	0.2102968354942405
2404	80-84	0.37948029523406746
2404	85-89	0.30750751860936987
2404	90-94	0.3185342490581675
2404	95-99	0.44721512104135996
2404	100-104	0.26998073002146583
2404	105-109	0.32353940058128217
2404	110-114	0.3046893212446591
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2404	125-129	0.2433043247396256
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2404	135-139	0.31655732941123205
2404	140-144	0.43952721607560363
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2404	150-154	0.4721889835710229
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2404	175-179	0.3181348661233727
2404	180-184	0.2957577716390247
2404	185-189	0.29029348347509654
2404	190-194	0.44054962890717064
2404	195-199	0.29935637832015516
2404	200-204	0.49287133292893515
2404	205-209	0.36345802496234114
2404	210-214	0.6669922912952089
2404	215-219	0.4304138490646139
2404	220-224	0.34104695467718926
2404	225-229	0.37609635754240855
2404	230-234	0.334410035758296
2404	235-239	0.4245656029257674
2404	240-244	0.3749000160597902
2404	245-249	1.1549255615410203
2404	250-254	0.4683013948443602
2404	255-259	0.43865403184987173
2404	260-264	0.41423200429973406
2404	265-269	0.47118779113307596
2404	270-274	0.577883450910015
2404	275-279	0.7355239398457982
2404	280-284	0.6411690766417877
2404	285-289	0.619756472716368
2404	290-294	0.9410434434344808
2404	295-299	0.6921384820379757
2404	300-301	2.839445866176675
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	79.0
21	117.0
22	152.0
23	176.0
24	229.0
25	267.0
26	291.0
27	308.0
28	369.0
29	421.0
30	547.0
31	664.0
32	786.0
33	1056.0
34	1349.0
35	1971.0
36	2882.0
37	5069.0
38	14029.0
39	113627.0
40	1943.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	99.76560631427887	0.20022550996002322	0.02391772303276728	0.010250452728328835
2	0.30683021833464313	99.42255782963747	0.009567089213106911	0.2610448628147743
3	0.12710561383127755	0.08268698534185259	0.19407523832302595	99.59613216250385
4	0.11002152595072949	0.13393924898349677	99.58929852735162	0.16674069771414904
5	0.18860833020125056	98.83213175248574	0.07311989612874568	0.906140021184269
6	0.1072880718898418	0.25831140875388664	0.1312057949226091	99.50319472443367
7	0.263778316875662	31.242013188915845	37.61984487648205	30.87436361772645
8	34.39710253869546	33.36180681313425	0.11890525164861447	32.12218539652168
9	99.73895513718523	0.17357433286636828	0.04031844739809341	0.04715208255031264
10-14	39.984145966446846	19.841186319062427	39.85471691666382	0.31995079782690405
15-19	0.11945194246079202	39.75439915262924	39.7847404927051	20.341408412204874
20-24	19.99589981890867	0.09526087402193598	39.8636006423617	40.04523866470769
25-29	20.042231865240716	0.3097003450985752	40.40728465507227	39.24078313458844
30-34	0.21348276215532852	38.65541394744935	0.4808145693101445	60.650288721085175
35-39	20.02159428708101	0.25612464550517644	58.72101684491064	21.00126422250316
40-44	39.50702156011891	19.45973280486555	20.173984350975502	20.859261284040045
45-49	0.7634537192059315	19.92770014008952	20.094714183209756	59.214131957494786
50-54	39.732394847439096	0.4260088153893464	20.15895035364062	39.68264598353094
55-59	39.13650186216558	20.449106502203847	39.52191888475075	0.8924727508798306
60-64	20.515529435883415	38.32111251580278	1.142173779341921	40.02118426897188
65-69	40.00396350838828	39.614172959305705	0.4082413639935764	19.97362216831243
70-74	40.28181911367752	19.45385587863464	0.39389073017391596	39.87043427751392
75-79	0.43051901458981107	0.12696894112823315	39.93767724741176	59.5048347968702
80-84	19.87516264118348	20.08960299150439	0.5502465585672268	59.484987808744904
85-89	59.54479229593831	0.42888421766215734	39.30522943528878	0.7210940511107526
90-94	0.21785956395285266	0.0623236895624221	78.54329309628464	21.176523650200092
95-99	0.8573595839626058	39.44510124168848	19.897494071740482	39.800045102608436
100-104	0.12724402561281462	19.938359768473276	59.431160435445186	20.503235770468724
105-109	20.17822364060055	39.6144410351732	0.4582698366055503	39.7490654876207
110-114	0.135444499873576	19.928519199360363	40.461823375451885	39.47421292531418
115-119	19.98783596317987	0.4653769142982102	19.88724347891453	59.65954364360739
120-124	0.3060156192340179	58.56269698303315	0.6982732463897263	40.4330141513431
125-129	58.940354809611016	21.31085476860837	19.423229368832516	0.3255610529480907
130-134	0.42697836269379064	19.558998596329133	39.925210448122264	40.08881259285481
135-139	0.28593101845840535	0.5411094178187509	39.06266016989113	60.11029939383171
140-144	0.22100882258472349	0.516780679154508	58.613261896138155	40.648948602122616
145-149	20.24820780569812	20.361240765671877	19.663087972992365	39.72746345563764
150-154	0.491773253986261	39.440816360003176	20.215325065127836	39.85208532088273
155-159	20.346922094927244	19.7837990033268	39.36749795662859	20.501780945117368
160-164	20.309665991003612	38.76497176840111	20.278775933638315	20.64658630695696
165-169	19.63417767887897	1.8487941695871584	58.19081699042662	20.326211161107256
170-174	20.5520700919889	39.297029838301825	19.72485340550293	20.42604666420634
175-179	0.7841609600743565	19.271879826683616	58.55496781071883	21.388991402523203
180-184	40.12766364593158	1.0133814463990376	20.449419772829785	38.4095351348396
185-189	39.84256574909754	20.1401845526355	19.67805119415205	20.339198504114915
190-194	40.47976708743106	38.459004517465026	0.5012267548745566	20.56000164022936
195-199	19.59562436696706	0.6514515505120989	59.689009112940596	20.063914969580246
200-204	0.3716542235174154	39.537639729139386	19.67703398332955	40.413672064013646
205-209	20.104702738537032	39.054394849609416	1.1956068589861877	39.64529555286737
210-214	1.0177591963581887	20.738735243661015	76.98004239975457	1.2634631602262236
215-219	19.736507563310084	39.71974881007384	0.41391113213169706	40.129832494484376
220-224	38.77876577974315	0.8034939273192037	19.979495731694815	40.43824456124283
225-229	1.8909036231042096	19.478234719194045	19.49696195091278	59.13389970678896
230-234	19.24298241417255	0.5244991832466459	59.71157329250706	20.520945110073747
235-239	19.903219853599524	19.881075243829923	0.20121521963488734	60.01448968293567
240-244	0.9530409963461294	59.255993755749756	19.977636616895836	19.81332863100828
245-249	0.8343323946803469	39.6397802994396	21.11815875317144	38.40772855270862
250-254	39.09603515798538	40.37304098803234	0.9527650005809543	19.578158853401316
255-259	20.071218158580013	19.819972524280473	20.19109978060433	39.91770953653519
260-264	20.165264402539794	19.68833512176118	19.498328902133128	40.6480715735659
265-269	0.6039198746777408	19.891217873185028	20.174587248514804	59.330275003622425
270-274	19.601911568103713	20.33898073395248	59.611288968841336	0.4478187291024761
275-279	20.559364085599654	19.69010792227409	0.2863801953399996	59.46414779678625
280-284	59.28124722334238	0.2058656678673219	19.975394541689166	20.537492567101133
285-289	40.0002187163809	0.6176003805665028	19.97085604224507	39.411324860807525
290-294	20.97944732651206	39.28479156841436	19.44636962004552	20.28939148502806
295-299	39.405681926985736	20.030639182590377	39.800298184902	0.763380705521892
300-301	3.1816783500566173	2.2947879644720217	0.3953371165262697	94.12819656894509
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	2.333333333333333
31	1.9999999999999998
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.3333333333333333
41	0.0
42	1.8333333333333333
43	2.4166666666666665
44	4.0
45	8.166666666666666
46	11.5
47	15.666666666666666
48	19.749999999999996
49	44.083333333333336
50	118.1666666666666
51	2461.7499999999973
52	31340.749999999844
53	11392.500000000004
54	508.1666666666664
55	295.08333333333337
56	567.4166666666665
57	274.00000000000006
58	120.08333333333331
59	76.33333333333334
60	47.083333333333336
61	23.0
62	14.416666666666666
63	7.916666666666666
64	4.166666666666667
65	3.5
66	2.5
67	1.4166666666666665
68	0.9166666666666665
69	0.25
70	0.25
71	0.5
72	0.75
73	0.75
74	0.25
75	0.25
76	0.5
77	0.0
78	0.5
79	1.0
80	1.0
81	0.5
82	0.5
83	1.0
84	0.75
85	0.5
86	0.75
87	0.75
88	1.5
89	1.0
90	1.25
91	1.0
92	1.0
93	1.25
94	1.5
95	1.0
96	1.0
97	1.0
98	1.75
99	10.583333333333334
100	14.333333333333341
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	1.3667457101269022E-4
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	4.100371630348764E-4
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	1.3668764822066855E-4
205-209	0.0
210-214	0.6428428882686299
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	2.7339024406914037E-4
245-249	1.9431211597214153
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	1.3669661689542846E-4
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
80-84	1.0
85-89	1.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	1.0
125-129	0.0
130-134	3.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	2.0
155-159	1.0
160-164	3.0
165-169	1.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	1.0
190-194	1.0
195-199	1.0
200-204	0.0
205-209	0.0
210-214	7.0
215-219	1.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	1.0
270-274	1.0
275-279	0.0
280-284	0.0
285-289	1.0
290-294	3.0
295-299	612.0
300-302	145693.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	3.850753408275532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.66637089618456	2.6056650835411896
2	10.062111801242237	0.7749342262616599
3	4.56078083407276	0.5268732702361021
4	2.271517302573203	0.3498821197936242
5	1.6149068322981366	0.31093039942597467
6	1.3309671694764862	0.30751358184986505
7	1.1535048802129548	0.31093039942597467
8	0.9228039041703637	0.2842792223323197
9	0.7985803016858917	0.2767622236648785
>10	8.553682342502219	6.87190350907165
>50	0.6743566992014197	1.8013462261249873
>100	0.23070097604259093	1.3168414938326443
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.15971606033717836	84.26213824443913
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	15948	10.898281340759217	No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	15395	10.520381316841494	No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	15259	10.427443878771312	No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	13325	9.105818840332116	No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	13200	9.020398400929375	No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	13077	8.936344688557078	No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	12947	8.847507431578228	No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	12292	8.39990432910787	No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	11862	8.106058017562443	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	229	0.1564902449858202	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	210	0.1435063381966037	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	171	0.1168551611029487	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	156	0.10660470837461988	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	155	0.10592134485939794	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	147	0.10045443673762258	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
