FastQCFastQC Report
Fri 1 Nov 2024
JAMS2_cleaned_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJAMS2_cleaned_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146335
Sequences flagged as poor quality0
Sequence length81-301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1836312.54860423002016No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1733211.844056445826357No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1643111.228345918611407No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1640911.213311921276523No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1579510.793726722930263No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1494010.20945091741552No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT142729.752964089247275No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT106687.290121980387466No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2350.16059042607715174No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2290.1564902449858202No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2270.15512351795537635No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2210.15102333686404484No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2170.14828988280315714No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2070.14145624765093792No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2020.1380394300748283No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1910.13052243140738715No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1770.12095534219428025No Hit
CCTACGGGCGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1560.10660470837461988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)