##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	JAMS1_cleaned_R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	161997
Sequences flagged as poor quality	0
Sequence length	86-301
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.058846768767324	40.0	40.0	40.0	40.0	40.0
2	39.01388297314148	40.0	40.0	40.0	40.0	40.0
3	39.38571701945098	40.0	40.0	40.0	40.0	40.0
4	39.31440705692081	40.0	40.0	40.0	40.0	40.0
5	38.374834101866085	40.0	40.0	40.0	40.0	40.0
6	39.0095372136521	40.0	40.0	40.0	40.0	40.0
7	38.792076396476475	40.0	40.0	40.0	40.0	40.0
8	38.98094409155725	40.0	40.0	40.0	40.0	40.0
9	39.40519886170731	40.0	40.0	40.0	40.0	40.0
10-14	39.24336376599567	40.0	40.0	40.0	40.0	40.0
15-19	39.26909757588104	40.0	40.0	40.0	40.0	40.0
20-24	39.40038395772761	40.0	40.0	40.0	40.0	40.0
25-29	39.260835694488165	40.0	40.0	40.0	40.0	40.0
30-34	38.72620357167108	40.0	40.0	40.0	36.8	40.0
35-39	39.43261171503176	40.0	40.0	40.0	40.0	40.0
40-44	39.301493237529094	40.0	40.0	40.0	40.0	40.0
45-49	39.17590572664926	40.0	40.0	40.0	40.0	40.0
50-54	39.362338808743374	40.0	40.0	40.0	40.0	40.0
55-59	39.17653289875738	40.0	40.0	40.0	40.0	40.0
60-64	39.01617931196256	40.0	40.0	40.0	40.0	40.0
65-69	39.09945986654074	40.0	40.0	40.0	40.0	40.0
70-74	38.537686500367286	40.0	40.0	40.0	33.6	40.0
75-79	39.17102168558677	40.0	40.0	40.0	40.0	40.0
80-84	38.9872059359124	40.0	40.0	40.0	40.0	40.0
85-89	39.29082876916378	40.0	40.0	40.0	40.0	40.0
90-94	39.31750907429813	40.0	40.0	40.0	40.0	40.0
95-99	38.81871157312526	40.0	40.0	40.0	36.8	40.0
100-104	39.36943628237734	40.0	40.0	40.0	40.0	40.0
105-109	39.13839354058125	40.0	40.0	40.0	40.0	40.0
110-114	39.34952467962172	40.0	40.0	40.0	40.0	40.0
115-119	39.35028519222696	40.0	40.0	40.0	40.0	40.0
120-124	38.69764685547791	40.0	40.0	40.0	36.8	40.0
125-129	39.15479300193191	40.0	40.0	40.0	40.0	40.0
130-134	39.03445847187241	40.0	40.0	40.0	40.0	40.0
135-139	39.07308201931835	40.0	40.0	40.0	40.0	40.0
140-144	39.07506312156998	40.0	40.0	40.0	40.0	40.0
145-149	38.97598972769756	40.0	40.0	40.0	40.0	40.0
150-154	38.7588743106047	40.0	40.0	40.0	36.8	40.0
155-159	39.155017038882214	40.0	40.0	40.0	40.0	40.0
160-164	38.81157376826535	40.0	40.0	40.0	36.8	40.0
165-169	39.11040762530327	40.0	40.0	40.0	40.0	40.0
170-174	39.238768543154706	40.0	40.0	40.0	40.0	40.0
175-179	39.25693790242427	40.0	40.0	40.0	40.0	40.0
180-184	38.83785242025594	40.0	40.0	40.0	33.6	40.0
185-189	39.29175551124473	40.0	40.0	40.0	40.0	40.0
190-194	38.73590349842889	40.0	40.0	40.0	40.0	40.0
195-199	39.35503466327545	40.0	40.0	40.0	40.0	40.0
200-204	38.91409063690297	40.0	40.0	40.0	40.0	40.0
205-209	39.08846018507658	40.0	40.0	40.0	40.0	40.0
210-214	38.9144783223345	40.0	40.0	40.0	40.0	40.0
215-219	39.022123997604744	40.0	40.0	40.0	40.0	40.0
220-224	38.97306820917727	40.0	40.0	40.0	36.8	40.0
225-229	38.89635094174224	40.0	40.0	40.0	40.0	40.0
230-234	39.22253020304098	40.0	40.0	40.0	40.0	40.0
235-239	38.686324211202134	40.0	40.0	40.0	40.0	40.0
240-244	38.86818571860705	40.0	40.0	40.0	40.0	40.0
245-249	37.858317025440314	40.0	40.0	40.0	34.4	40.0
250-254	38.89698309123572	40.0	40.0	40.0	40.0	40.0
255-259	38.73433794069894	40.0	40.0	40.0	40.0	40.0
260-264	38.305039293276614	40.0	40.0	40.0	33.6	40.0
265-269	37.49646576577133	40.0	36.8	40.0	30.4	40.0
270-274	38.820591775883244	40.0	40.0	40.0	40.0	40.0
275-279	38.22977893287733	40.0	40.0	40.0	33.6	40.0
280-284	38.601359368344376	40.0	40.0	40.0	36.8	40.0
285-289	38.655808182138074	40.0	40.0	40.0	40.0	40.0
290-294	38.28844043040491	40.0	40.0	40.0	33.6	40.0
295-299	38.57317542546288	40.0	40.0	40.0	36.8	40.0
300-301	33.017324931475095	40.0	26.0	40.0	18.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.44904926598309913
1101	2	0.21608983484019717
1101	3	0.0024507131861497555
1101	4	-0.5526793952572788
1101	5	-0.4912157037699316
1101	6	-0.05338479631359405
1101	7	0.011971048817656538
1101	8	-0.09148164392428981
1101	9	-0.3781134626766942
1101	10-14	-0.09151194742772617
1101	15-19	-0.06621126917595177
1101	20-24	-0.11892627256787591
1101	25-29	-0.2019243346673747
1101	30-34	-0.15143407562493394
1101	35-39	-0.11721151037941979
1101	40-44	-0.0945201814168044
1101	45-49	-0.14809971853728854
1101	50-54	0.0042770745584235215
1101	55-59	-0.14482315588519157
1101	60-64	-0.29478075422099437
1101	65-69	0.018906652689011594
1101	70-74	-0.3397486086127657
1101	75-79	-0.1946844980075042
1101	80-84	-0.2501232986302284
1101	85-89	-0.09237609926373835
1101	90-94	-0.11758440387822588
1101	95-99	0.03720232758387709
1101	100-104	-0.11706728563653002
1101	105-109	-0.21120821163746228
1101	110-114	-0.09479226560785037
1101	115-119	-0.11139124557453783
1101	120-124	-0.18382182219728804
1101	125-129	-0.16031449387956798
1101	130-134	-0.3760021560232616
1101	135-139	-0.34984749254027037
1101	140-144	-0.2127788954393921
1101	145-149	-0.2486326957134537
1101	150-154	-0.0844349305123302
1101	155-159	-0.16765167874239495
1101	160-164	-0.07310610087629499
1101	165-169	-0.10403358000895935
1101	170-174	-0.10825175686723298
1101	175-179	0.01864243336036253
1101	180-184	-0.19717529958069235
1101	185-189	-0.14632934030105105
1101	190-194	-0.16791189296171183
1101	195-199	-0.22003098224710982
1101	200-204	-0.06605171555136735
1101	205-209	-0.13764212618093552
1101	210-214	0.18738647881105663
1101	215-219	-0.281403208642935
1101	220-224	0.032272135664285884
1101	225-229	-0.2968901380022899
1101	230-234	-0.25283022279927536
1101	235-239	-0.06634486254623795
1101	240-244	-0.20761798697371603
1101	245-249	0.6076977083261568
1101	250-254	-0.20332090975988848
1101	255-259	-0.4025969401872018
1101	260-264	-0.32972943508099206
1101	265-269	-0.4371213488982022
1101	270-274	-0.4312824725535691
1101	275-279	-0.1911544068948814
1101	280-284	-0.42527207944841905
1101	285-289	-0.5532450113610778
1101	290-294	-0.5670586884509135
1101	295-299	-0.5095124261846706
1101	300-301	-1.2487200267929097
1102	1	-0.8089346048402533
1102	2	-0.09546206606579233
1102	3	-0.20331163694881127
1102	4	-0.11797138362377524
1102	5	-0.7280704732968459
1102	6	-0.23148619191546516
1102	7	0.07256569656941991
1102	8	-0.2626782626759834
1102	9	-0.12899748334918115
1102	10-14	-0.24775240105064
1102	15-19	-0.17114713844550522
1102	20-24	-0.197975505719171
1102	25-29	-0.08896218539865686
1102	30-34	-0.31828696203088924
1102	35-39	-0.14508170057463587
1102	40-44	-0.11256466491857964
1102	45-49	-0.36339484543871947
1102	50-54	-0.3019769793825233
1102	55-59	-0.31360937980920767
1102	60-64	-0.39789208851681224
1102	65-69	-0.3620780103369583
1102	70-74	-0.7036429027562647
1102	75-79	-0.41916925518951587
1102	80-84	-0.516580729634768
1102	85-89	-0.3995173124121152
1102	90-94	-0.31386419380562103
1102	95-99	-0.3872531366054375
1102	100-104	-0.4464209566762207
1102	105-109	-0.3971517598558236
1102	110-114	-0.2608249482182785
1102	115-119	-0.43791605046227744
1102	120-124	-0.38257896236425637
1102	125-129	-0.3638338377165269
1102	130-134	-0.3806137100165117
1102	135-139	-0.45759548910880454
1102	140-144	-0.39900700415824275
1102	145-149	-0.39658741711617296
1102	150-154	-0.40204629619350385
1102	155-159	-0.43107100793286435
1102	160-164	-0.6585223752260916
1102	165-169	-0.21307046825618414
1102	170-174	-0.4383251985846357
1102	175-179	-0.18321976403255746
1102	180-184	-0.22123740072520803
1102	185-189	-0.4224283506163715
1102	190-194	-0.519118136553864
1102	195-199	-0.3583524937655369
1102	200-204	-0.27788189988798706
1102	205-209	-0.41174921299280953
1102	210-214	-0.06952980600052427
1102	215-219	-0.48634535498234754
1102	220-224	-0.44378084280536
1102	225-229	-0.6238083059348298
1102	230-234	-0.41602566983127076
1102	235-239	-0.5684267573843798
1102	240-244	-0.6496994633405464
1102	245-249	-0.2968866450310159
1102	250-254	-0.6902708815130723
1102	255-259	-0.6817005746106517
1102	260-264	-0.823617661390287
1102	265-269	-1.133842626072692
1102	270-274	-0.6276375873193274
1102	275-279	-0.9722897195975833
1102	280-284	-0.9328108403548256
1102	285-289	-1.0478027917891737
1102	290-294	-0.8332064506755046
1102	295-299	-0.9085383813796852
1102	300-301	-2.918755990833226
1103	1	-0.1613887739709483
1103	2	0.07333070237216077
1103	3	-0.07109908309225688
1103	4	-0.11366285950448685
1103	5	-0.08294642722661649
1103	6	0.03462853309932257
1103	7	-0.01447775475552504
1103	8	-0.17738074922944946
1103	9	-0.12426655490447303
1103	10-14	-0.20351822009263287
1103	15-19	-0.12797741638755156
1103	20-24	-0.3574359545941732
1103	25-29	-0.15229467392332907
1103	30-34	0.026073940224755177
1103	35-39	-0.10719484861327544
1103	40-44	-0.10966978727502408
1103	45-49	-0.035592192739549944
1103	50-54	-0.17473190039291353
1103	55-59	-0.2322036182142
1103	60-64	-0.24372014179607504
1103	65-69	-0.35629951916520497
1103	70-74	-0.4998975844042022
1103	75-79	-0.12010699279194625
1103	80-84	-0.22922188308017155
1103	85-89	-0.2833955473252274
1103	90-94	-0.11762640833070037
1103	95-99	-0.43378519709373364
1103	100-104	-0.16479554483200332
1103	105-109	-0.16739196711554172
1103	110-114	-0.18608754290725216
1103	115-119	-0.17293336903962597
1103	120-124	-0.33938671207560844
1103	125-129	-0.24063820819327475
1103	130-134	-0.358654317152336
1103	135-139	-0.4238369354212139
1103	140-144	-0.24161915250663668
1103	145-149	-0.37484767450121126
1103	150-154	-0.29246898212180383
1103	155-159	-0.3231213585284536
1103	160-164	-0.4188839533286597
1103	165-169	-0.333061456963911
1103	170-174	-0.30304880535436496
1103	175-179	-0.30402176904509304
1103	180-184	-0.46549974197158406
1103	185-189	-0.43880074226964894
1103	190-194	-0.4176115611265345
1103	195-199	-0.2810074457522447
1103	200-204	-0.18534156027490667
1103	205-209	-0.3656282927145966
1103	210-214	-0.14316896006545932
1103	215-219	-0.586663566240837
1103	220-224	-0.21024757142465944
1103	225-229	-0.7761329377390496
1103	230-234	-0.3303564405783561
1103	235-239	-0.3664442730837436
1103	240-244	-0.44894476761246693
1103	245-249	0.3014068414942841
1103	250-254	-0.31219084997356106
1103	255-259	-0.3657590353692868
1103	260-264	-0.5053444502726094
1103	265-269	-0.7400322574545086
1103	270-274	-0.5953602647995524
1103	275-279	-0.5074647357897462
1103	280-284	-0.5580593548996191
1103	285-289	-0.7966468119519448
1103	290-294	-0.7752131528241293
1103	295-299	-0.9121196382099512
1103	300-301	-2.592130274759292
1104	1	-0.26235129701006343
1104	2	0.009552580023488133
1104	3	-0.06445796668255355
1104	4	-0.07337732075488645
1104	5	0.11251303855846828
1104	6	-0.16975401861069628
1104	7	-0.2524754727860099
1104	8	-0.11913201455229228
1104	9	0.013353760475418142
1104	10-14	-0.1805815008626439
1104	15-19	-0.03405974282621571
1104	20-24	-0.04453112442536167
1104	25-29	-0.17170311721054077
1104	30-34	-0.39766439393750375
1104	35-39	-0.15605825225857473
1104	40-44	-0.09038421926940998
1104	45-49	-0.2841922922100224
1104	50-54	-0.13204736805038664
1104	55-59	-0.17921511582983385
1104	60-64	-0.20918633646563478
1104	65-69	-0.2649154168861614
1104	70-74	-0.43452793459875494
1104	75-79	-0.10825245372053871
1104	80-84	-0.2365241855289142
1104	85-89	-0.027206436415660562
1104	90-94	-0.23140306787839648
1104	95-99	-0.33034761481118124
1104	100-104	-0.14944198583724244
1104	105-109	-0.05956013071520516
1104	110-114	-0.10517709558271093
1104	115-119	-0.20424316102932494
1104	120-124	-0.277943220077141
1104	125-129	-0.23270397572494517
1104	130-134	-0.38990559710318706
1104	135-139	-0.31052252098044875
1104	140-144	-0.31398099095489584
1104	145-149	-0.33344455580954246
1104	150-154	-0.3869659248677735
1104	155-159	-0.3176621853035613
1104	160-164	-0.39144765831095896
1104	165-169	-0.42465035137892926
1104	170-174	-0.26931661495618897
1104	175-179	-0.28957514665253825
1104	180-184	-0.2606842011740582
1104	185-189	-0.35686633426521297
1104	190-194	-0.5688944375783649
1104	195-199	-0.08232981783029913
1104	200-204	-0.2554799412467972
1104	205-209	-0.3499529175061511
1104	210-214	-0.10330470799559066
1104	215-219	-0.41337431197678143
1104	220-224	-0.2741368666471473
1104	225-229	-0.6237441837529332
1104	230-234	-0.18458539818638542
1104	235-239	-0.41405551909761584
1104	240-244	-0.48935903284753834
1104	245-249	0.2831159781491479
1104	250-254	-0.353722695150708
1104	255-259	-0.6007492346027803
1104	260-264	-0.643824892623627
1104	265-269	-0.7372061259779628
1104	270-274	-0.39784117524687446
1104	275-279	-0.4180700428316726
1104	280-284	-0.837717644592729
1104	285-289	-0.5899237786878473
1104	290-294	-0.6656493600737718
1104	295-299	-0.5179002199375233
1104	300-301	-3.0698094163907683
1201	1	4.045983487444005E-4
1201	2	0.1401644534164319
1201	3	-0.09699716162191407
1201	4	-0.3010758363911492
1201	5	-0.12443904766660552
1201	6	-0.06283735384025135
1201	7	-0.6140321964152307
1201	8	-0.23056927610218736
1201	9	-0.20613789993371512
1201	10-14	-0.07642270352342706
1201	15-19	-0.08548228968189875
1201	20-24	-0.0505019529233266
1201	25-29	-0.11581458481166607
1201	30-34	-0.054739203746287046
1201	35-39	-0.1512494295099529
1201	40-44	-0.09351829743933138
1201	45-49	0.10971552098921222
1201	50-54	-0.08523036878612089
1201	55-59	-0.03459849429328443
1201	60-64	-0.2115285517097547
1201	65-69	-0.12215861134009032
1201	70-74	-0.04375723352701044
1201	75-79	-0.13347374300080617
1201	80-84	-0.03785457592339725
1201	85-89	-0.005255753395964291
1201	90-94	-0.13806639719173575
1201	95-99	-0.10719093365736398
1201	100-104	-0.14786432790078408
1201	105-109	-0.15029366580672132
1201	110-114	-0.19243674925645138
1201	115-119	-0.2449467357898314
1201	120-124	-0.1578773831978566
1201	125-129	-0.03679526750823214
1201	130-134	-0.18672449259093327
1201	135-139	-0.29429084798039185
1201	140-144	-0.18542310683691454
1201	145-149	-0.16766304626395367
1201	150-154	-0.14532334023931526
1201	155-159	-0.08290971831682015
1201	160-164	-0.24483772665085013
1201	165-169	-0.21983394373638987
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2404	280-284	0.7096909457073863
2404	285-289	0.7466333537803109
2404	290-294	0.7109038738465898
2404	295-299	0.6074365920999867
2404	300-301	3.6250661779249214
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	75.0
21	123.0
22	183.0
23	163.0
24	186.0
25	268.0
26	308.0
27	317.0
28	404.0
29	522.0
30	574.0
31	657.0
32	862.0
33	1028.0
34	1483.0
35	2020.0
36	3133.0
37	5439.0
38	15019.0
39	126712.0
40	2519.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	99.75987209639685	0.219757155996716	0.010494021494225202	0.009876726112211954
2	0.2944498972203189	99.40677913788527	0.017901566078384168	0.28086939881602746
3	0.11296505490842423	0.10123644265017254	0.19074427304209338	99.59505422939931
4	0.11543423643647721	0.13950875633499388	99.50986746668148	0.23518954054704716
5	0.18395402383994766	98.61664104890832	0.07839651351568239	1.1210084137360568
6	0.11419964567245074	0.2253128144348352	0.14876818706519257	99.51171935282753
7	0.24815274356932535	31.683302777211924	37.32291338728495	30.745631091933802
8	34.44878608863127	33.355555967085806	0.10555751032426527	32.09010043395865
9	99.75308184719471	0.15555843626733828	0.043827972122940546	0.047531744415020026
10-14	39.980863843157586	19.844441563732662	39.85419483076847	0.3204997623412779
15-19	0.12469366716667593	39.75740291486879	39.78320586183695	20.334697556127583
20-24	20.028889423878223	0.06580368772261215	39.94431995654241	39.96098693185676
25-29	20.041852626900496	0.2735853133082711	40.078026136286475	39.60653592350475
30-34	0.18704050075001388	39.20072593936925	0.3879084180571245	60.22432514182361
35-39	20.028395587572607	0.13197775267443224	59.26875189046711	20.57087476928585
40-44	39.714192238127865	19.844565022809064	19.915800909893395	20.525441829169676
45-49	0.6858151694167176	19.859997407359394	19.976419316407092	59.4777681068168
50-54	39.74357549831169	0.4166743828589418	20.047902121644228	39.79184799718513
55-59	39.56159681969419	19.8787631869726	39.831107983481175	0.7285320098520343
60-64	20.262227078279228	39.0356611542189	0.666308635345099	40.035803132156765
65-69	40.17852182447823	39.80481120020741	0.10074260634456193	19.915924368969794
70-74	40.24926387525694	19.561967196923398	0.11827379519373815	40.07049513262591
75-79	0.4193904825398001	0.07197664154274462	39.87357790576368	59.63505497015377
80-84	19.876294005444546	19.997407359395545	0.33630252412081707	59.78999611103909
85-89	59.61589269885009	0.3088957532389609	39.6201397068083	0.4550718411026418
90-94	0.17617718956023606	0.04222326477196968	78.93725771006692	20.84434183560088
95-99	0.40655324822835126	39.72888219462209	20.077038939233066	39.787525617916494
100-104	0.11642262771920295	20.02642040544211	59.451714857156965	20.405442109681722
105-109	20.093335637916987	39.55554458134769	0.42667720190621994	39.92444257882911
110-114	0.13062050914837403	19.92567717721425	39.94431961282995	39.99938270080743
115-119	20.12975629027877	0.42717104126027805	19.757154497641917	59.68591817081903
120-124	0.25691992394873947	59.09590360255809	0.5297661670658536	40.11741030642732
125-129	59.46401079534161	20.59102766861857	19.79878490676919	0.1461766292706354
130-134	0.24976572888257306	19.772482310833016	39.85732611156861	40.120425848715804
135-139	0.1019818556252994	0.24680596779052483	39.38363944351983	60.26757273306435
140-144	0.0884010642697961	0.3228614288624536	59.247973627838924	40.34076387902882
145-149	20.293353252381337	20.139145250603434	19.789985739772455	39.777515757242774
150-154	0.45546583697557846	39.708743857569694	19.984072894288467	39.85171741116626
155-159	20.060523076429213	19.932982607332157	39.78398373210822	20.22251058413041
160-164	20.143097902918136	39.26228647977924	20.193472315679653	20.40114330162297
165-169	19.80331755017378	1.061443202232278	58.93472933013143	20.20050991746251
170-174	20.284343805367097	39.64490977671048	19.93246371622414	20.138282701698284
175-179	0.4220091735756573	19.528233747152548	59.16030298727676	20.889454091995034
180-184	40.050004012667685	0.5149795971281645	20.375832628544266	39.05918376165989
185-189	40.02469333958898	19.962589590522697	19.687135387407633	20.325581682480692
190-194	40.45460438183311	39.16906912283085	0.18347151314611665	20.192854982189928
195-199	19.72084179594659	0.3628686252600517	59.65688604641113	20.25940353238223
200-204	0.19878138369128387	39.687629254199415	19.942464518757678	40.17112484335163
205-209	20.10334162617988	39.436991857371275	0.8600690178841512	39.5995974985647
210-214	0.6788336584214384	20.4620392342001	78.09643314159347	0.7626939657849946
215-219	19.793440214338187	39.953206121478885	0.23680912665831208	40.01654453752462
220-224	39.34649076777766	0.330273417002599	19.985677863038394	40.33755795218135
225-229	1.116262416119812	19.602437232617433	19.91122744417762	59.37007290708514
230-234	19.562927889275063	0.4580614493755672	59.6829375196775	20.296073141671865
235-239	19.97049145919117	19.80578688413268	0.1554445727126251	60.06827708396353
240-244	0.5137449301487156	59.707383925870595	19.97654132739047	19.80232981659022
245-249	0.8036834758397768	40.02947888332681	20.717034187700683	38.44980345313273
250-254	39.374147308117315	40.22224005630081	0.6362238945100532	19.767388741071816
255-259	20.07766055300734	19.787143412742996	20.212362720465222	39.92283331378444
260-264	20.051115212949185	19.856161296894197	19.63885990851118	40.453863581645436
265-269	0.5206590652336298	20.217054454987128	20.01765573780612	59.244630741973126
270-274	19.624661238247516	20.35780649064431	59.64404550982486	0.3734867612833129
275-279	20.499669726582997	19.655404446035792	0.23322859241791008	59.6116972349633
280-284	59.35105903560163	0.19594164963855124	19.980615728422652	20.472383586337177
285-289	39.98839413039318	0.5652305431917376	19.967651725138438	39.47872360127664
290-294	20.766357794143975	39.269941415051825	19.766154074092366	20.197546716711834
295-299	39.47694109102767	20.019836417488655	39.80536596090521	0.6978565305784674
300-301	2.7714928767822893	1.9686091464609572	0.42460197276608885	94.83529600399066
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.8333333333333333
44	0.5
45	0.0
46	0.5
47	3.333333333333333
48	7.083333333333334
49	13.333333333333334
50	71.25000000000001
51	2664.500000000002
52	35462.666666666555
53	13041.16666666667
54	441.41666666666663
55	165.16666666666666
56	451.3333333333333
57	154.0
58	6.5
59	2.25
60	1.25
61	1.0
62	0.75
63	1.25
64	0.25
65	0.25
66	1.0
67	0.75
68	0.0
69	0.75
70	1.0
71	0.5
72	0.25
73	1.0
74	1.5
75	0.75
76	0.25
77	1.0
78	0.5
79	0.5
80	0.5
81	0.25
82	1.5
83	1.25
84	1.25
85	0.75
86	1.25
87	1.0
88	1.5
89	1.25
90	0.75
91	1.25
92	1.75
93	1.25
94	1.0
95	1.0
96	1.25
97	0.5
98	1.75
99	10.083333333333332
100	16.50000000000001
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.2346578331014235E-4
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.6204201571731065
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	1.909782883811664
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
85-89	1.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	3.0
130-134	3.0
135-139	1.0
140-144	0.0
145-149	0.0
150-154	1.0
155-159	1.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	2.0
295-299	477.0
300-302	161508.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	2.667950641061254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.91346598796854	1.7852182447823106
2	8.144377602961592	0.434575948937326
3	4.095326237852846	0.32778384784903425
4	1.5733456732993985	0.16790434390760323
5	1.5270708005552984	0.2037074760643716
6	1.2956964368347987	0.20741124835645103
7	1.0411846367422488	0.19444804533417284
8	1.064322073114299	0.22716470058087496
9	0.8329477093937991	0.20000370377229207
>10	11.892642295233689	6.584072544553295
>50	1.018047200370199	1.7506497033895692
>100	0.3933364183248496	1.5969431532682703
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.20823692734844979	86.32011703920442
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	18022	11.12489737464274	No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	17339	10.703284628727692	No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	17093	10.551429964752433	No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	15275	9.42918696025235	No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	15107	9.325481336074125	No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	14741	9.099551226257278	No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	14716	9.084118841706946	No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	14209	8.771150083026228	No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	13334	8.231016623764638	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	205	0.12654555331271566	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	204	0.12592825793070242	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	190	0.11728612258251696	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	189	0.11666882720050371	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	175	0.10802669185231825	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	172	0.10617480570627852	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	171	0.10555751032426527	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	164	0.10123644265017254	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
