##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	JAMS1_cleaned_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	161997
Sequences flagged as poor quality	0
Sequence length	86-301
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.69024117730575	40.0	40.0	40.0	40.0	40.0
2	39.06285918875041	40.0	40.0	40.0	40.0	40.0
3	39.214528664111064	40.0	40.0	40.0	40.0	40.0
4	38.923288702877215	40.0	40.0	40.0	40.0	40.0
5	39.11344037235258	40.0	40.0	40.0	40.0	40.0
6	39.32327141860652	40.0	40.0	40.0	40.0	40.0
7	39.35695105464916	40.0	40.0	40.0	40.0	40.0
8	39.41292739989012	40.0	40.0	40.0	40.0	40.0
9	38.94595578930474	40.0	40.0	40.0	40.0	40.0
10-14	39.56665370346364	40.0	40.0	40.0	40.0	40.0
15-19	39.61391383791057	40.0	40.0	40.0	40.0	40.0
20-24	39.622313993469014	40.0	40.0	40.0	40.0	40.0
25-29	39.66675926097397	40.0	40.0	40.0	40.0	40.0
30-34	39.362086952227514	40.0	40.0	40.0	40.0	40.0
35-39	39.59834317919467	40.0	40.0	40.0	40.0	40.0
40-44	39.385613313826795	40.0	40.0	40.0	40.0	40.0
45-49	39.602456835620416	40.0	40.0	40.0	40.0	40.0
50-54	39.57332543195244	40.0	40.0	40.0	40.0	40.0
55-59	39.442295844984784	40.0	40.0	40.0	40.0	40.0
60-64	39.456873892726406	40.0	40.0	40.0	40.0	40.0
65-69	39.55622881905221	40.0	40.0	40.0	40.0	40.0
70-74	39.37410692790608	40.0	40.0	40.0	40.0	40.0
75-79	39.2402427205442	40.0	40.0	40.0	36.8	40.0
80-84	39.68545096514133	40.0	40.0	40.0	40.0	40.0
85-89	39.6316806858984	40.0	40.0	40.0	40.0	40.0
90-94	39.331000765451	40.0	40.0	40.0	40.0	40.0
95-99	39.360013827501916	40.0	40.0	40.0	40.0	40.0
100-104	39.24103805032223	40.0	40.0	40.0	40.0	40.0
105-109	39.33863058347119	40.0	40.0	40.0	40.0	40.0
110-114	39.143820835082344	40.0	40.0	40.0	40.0	40.0
115-119	39.09423936393492	40.0	40.0	40.0	40.0	40.0
120-124	39.184251462999086	40.0	40.0	40.0	40.0	40.0
125-129	39.042696617848456	40.0	40.0	40.0	40.0	40.0
130-134	39.151886173460205	40.0	40.0	40.0	40.0	40.0
135-139	39.2895942314632	40.0	40.0	40.0	40.0	40.0
140-144	39.33321398366555	40.0	40.0	40.0	40.0	40.0
145-149	39.169133706609706	40.0	40.0	40.0	40.0	40.0
150-154	39.33864806503539	40.0	40.0	40.0	40.0	40.0
155-159	38.84914351246606	40.0	40.0	40.0	36.8	40.0
160-164	39.38002926160741	40.0	40.0	40.0	40.0	40.0
165-169	39.304112058375054	40.0	40.0	40.0	40.0	40.0
170-174	39.226500892046886	40.0	40.0	40.0	40.0	40.0
175-179	39.33948279800231	40.0	40.0	40.0	40.0	40.0
180-184	39.22917271139042	40.0	40.0	40.0	40.0	40.0
185-189	39.269916721712235	40.0	40.0	40.0	40.0	40.0
190-194	39.33657392259873	40.0	40.0	40.0	40.0	40.0
195-199	39.24803348416848	40.0	40.0	40.0	40.0	40.0
200-204	39.4992190731355	40.0	40.0	40.0	40.0	40.0
205-209	38.69623611771315	40.0	40.0	40.0	36.8	40.0
210-214	39.16554291393754	40.0	40.0	40.0	40.0	40.0
215-219	39.15683110373054	40.0	40.0	40.0	40.0	40.0
220-224	39.287631723533366	40.0	40.0	40.0	40.0	40.0
225-229	39.362580947853836	40.0	40.0	40.0	40.0	40.0
230-234	38.98621000450653	40.0	40.0	40.0	40.0	40.0
235-239	39.34682165852816	40.0	40.0	40.0	40.0	40.0
240-244	39.30852228882564	40.0	40.0	40.0	40.0	40.0
245-249	39.24286763752647	40.0	40.0	40.0	40.0	40.0
250-254	39.21137375221468	40.0	40.0	40.0	40.0	40.0
255-259	39.27452449887954	40.0	40.0	40.0	40.0	40.0
260-264	39.35750893590226	40.0	40.0	40.0	40.0	40.0
265-269	39.38047868038793	40.0	40.0	40.0	40.0	40.0
270-274	39.12876959262163	40.0	40.0	40.0	36.8	40.0
275-279	39.202973078086515	40.0	40.0	40.0	40.0	40.0
280-284	37.64215153067838	40.0	36.8	40.0	36.8	40.0
285-289	39.20507201195157	40.0	40.0	40.0	40.0	40.0
290-294	39.350883712890536	40.0	40.0	40.0	40.0	40.0
295-299	39.187554810453314	40.0	40.0	40.0	40.0	40.0
300-301	39.25310340877708	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.08030825010342824
1101	2	0.10569261313280265
1101	3	-0.32484674607491826
1101	4	-0.23800840957537162
1101	5	-0.10895228450786476
1101	6	-0.09628923219482033
1101	7	-0.04212934940343871
1101	8	0.07599443771314895
1101	9	0.22677581849638528
1101	10-14	-0.01746384128227163
1101	15-19	-0.03297484970196507
1101	20-24	-0.05281785243561643
1101	25-29	0.057813553019855135
1101	30-34	0.08787658825510647
1101	35-39	0.13383907839087783
1101	40-44	0.18600139399143956
1101	45-49	0.08346466268545072
1101	50-54	0.24189271928631229
1101	55-59	-0.0012575501390443833
1101	60-64	-0.044205527299951086
1101	65-69	0.15263276519316804
1101	70-74	0.015761447574959675
1101	75-79	0.05426015598047229
1101	80-84	-0.010564112905420586
1101	85-89	0.07789213986375643
1101	90-94	-0.0835938950857269
1101	95-99	0.020062603708957738
1101	100-104	0.19040711789374143
1101	105-109	0.042578921584585316
1101	110-114	0.06385959904865501
1101	115-119	0.003641931848065383
1101	120-124	0.0773075636893168
1101	125-129	0.12365805944876485
1101	130-134	0.06411015220294303
1101	135-139	0.11175134277310406
1101	140-144	-0.0182628521666075
1101	145-149	-0.025303666471145903
1101	150-154	0.18632614339859543
1101	155-159	0.1628457995846091
1101	160-164	0.06848171682793947
1101	165-169	0.14810199687982362
1101	170-174	0.05959676843084338
1101	175-179	0.1638546338720488
1101	180-184	-0.03542073002402901
1101	185-189	0.09050058057182042
1101	190-194	0.034387094296683074
1101	195-199	0.09278918328539731
1101	200-204	0.10500430090407775
1101	205-209	0.12635955205642802
1101	210-214	0.16261309795265788
1101	215-219	-0.012535228564246381
1101	220-224	0.06467405595433462
1101	225-229	0.12015796521051669
1101	230-234	0.11178530010745646
1101	235-239	-0.07090234031417708
1101	240-244	-0.09635739331604043
1101	245-249	-0.06257499360949481
1101	250-254	0.06789645943176481
1101	255-259	0.06923971704559762
1101	260-264	-0.011889033263734916
1101	265-269	-0.03344363284767127
1101	270-274	-0.06162754104994406
1101	275-279	-0.0599975624421063
1101	280-284	-0.10999417800595523
1101	285-289	-0.14559579443849202
1101	290-294	-0.18483798269251395
1101	295-299	0.06587117299395828
1101	300-301	-0.1570536653107908
1102	1	0.01142787013146318
1102	2	0.19076783335334113
1102	3	-0.09142344140686731
1102	4	0.3162987065295013
1102	5	0.32717852962809246
1102	6	0.10164770417661373
1102	7	0.013903960913971503
1102	8	-0.08407084381343566
1102	9	0.15492429212223158
1102	10-14	-0.10540140536068776
1102	15-19	0.02904260055225194
1102	20-24	-0.06279420880579778
1102	25-29	-0.017143030580257346
1102	30-34	-0.11453799128583597
1102	35-39	-0.035566483093020906
1102	40-44	-0.03218955447382399
1102	45-49	-0.16708248857101893
1102	50-54	-0.12918254278135066
1102	55-59	-0.09733856618741754
1102	60-64	-0.13888884912532973
1102	65-69	-0.06329843812113012
1102	70-74	-0.1345701909495105
1102	75-79	-0.15796339140094062
1102	80-84	-0.06929237855101178
1102	85-89	-0.057106709340096984
1102	90-94	-0.21681005644826
1102	95-99	-0.040414872072013
1102	100-104	-0.10643970067800979
1102	105-109	-0.1453649195636899
1102	110-114	-0.1368727257687894
1102	115-119	-0.03593708599973411
1102	120-124	-0.06772622787012494
1102	125-129	0.0020837703269336316
1102	130-134	-0.04217319473070802
1102	135-139	0.03636791842225051
1102	140-144	0.004794917799706866
1102	145-149	0.025247669498554615
1102	150-154	0.08022692384761143
1102	155-159	0.12268929130934225
1102	160-164	0.04280431637180726
1102	165-169	0.08197515822144652
1102	170-174	-0.05079596690696775
1102	175-179	-0.0022701124297057618
1102	180-184	-0.08650770928877449
1102	185-189	-0.06753093031888824
1102	190-194	-0.07103420170900421
1102	195-199	-0.08785651796792848
1102	200-204	-0.0036810714307833337
1102	205-209	-0.09450961288404613
1102	210-214	0.004489523370608595
1102	215-219	-0.044472960031207265
1102	220-224	0.12545283108876504
1102	225-229	0.021356885554489224
1102	230-234	-0.04023696256700049
1102	235-239	-0.1094853964102569
1102	240-244	-0.1309063858463304
1102	245-249	-0.18563067121917953
1102	250-254	-0.0022142262334980956
1102	255-259	-0.054435298123983955
1102	260-264	-0.1025131832438575
1102	265-269	-0.03260669019916662
1102	270-274	-0.03858650993660717
1102	275-279	-0.12774806983858866
1102	280-284	-0.12849061053791644
1102	285-289	-0.05959993730460411
1102	290-294	-0.10500620816486617
1102	295-299	-0.216063942487267
1102	300-301	-0.19428955456400132
1103	1	0.017707614436048402
1103	2	-0.06777177409224322
1103	3	0.31878747915295946
1103	4	0.2607384575925522
1103	5	0.06928660881482784
1103	6	0.18221428917844662
1103	7	0.24459304317016972
1103	8	0.24430627876829902
1103	9	-0.013727674873912576
1103	10-14	0.038430083473748766
1103	15-19	0.09684131081105107
1103	20-24	0.1084372406042462
1103	25-29	-0.12823536816579661
1103	30-34	0.05100507987521041
1103	35-39	-0.0673594486530007
1103	40-44	0.017975183213096102
1103	45-49	0.12246159636040943
1103	50-54	0.050682851040278365
1103	55-59	-0.06976401182398462
1103	60-64	0.03599217365634644
1103	65-69	0.041519787217502824
1103	70-74	0.0339409746048176
1103	75-79	0.006993945762182818
1103	80-84	0.010485718376699538
1103	85-89	-0.11286408029558004
1103	90-94	-0.11046554348045134
1103	95-99	-0.018956732340662086
1103	100-104	0.07525611188710712
1103	105-109	0.06199617981396699
1103	110-114	-0.1045334506146034
1103	115-119	-0.05003975841109565
1103	120-124	-0.21544946855610192
1103	125-129	-0.06168238663851611
1103	130-134	-0.18387013370735872
1103	135-139	-0.005337468640910004
1103	140-144	-0.10450079338838236
1103	145-149	-0.009406381358921578
1103	150-154	-0.2032007464517278
1103	155-159	-0.06924560210397601
1103	160-164	-0.033121453108080345
1103	165-169	-0.07530568433438134
1103	170-174	-0.052401103343569844
1103	175-179	-0.18072749183328085
1103	180-184	-0.21311636576141524
1103	185-189	-0.13076767386314714
1103	190-194	-0.1850833695724745
1103	195-199	-0.05503288585393307
1103	200-204	-0.02970117787667448
1103	205-209	-0.14188648202319598
1103	210-214	0.010363759522782345
1103	215-219	-0.14566414022888807
1103	220-224	-0.0887850107067365
1103	225-229	-0.07420223697091899
1103	230-234	-0.030758209504568867
1103	235-239	-0.1259253727556171
1103	240-244	-0.22132343971696145
1103	245-249	0.012262939211382218
1103	250-254	-0.19024610367992523
1103	255-259	-0.11159505143986337
1103	260-264	-0.021141213994887664
1103	265-269	-0.16738183029660547
1103	270-274	-0.24363283361095966
1103	275-279	-0.09514408797381435
1103	280-284	0.028987354036438262
1103	285-289	-0.03130447955911819
1103	290-294	-0.08010688119575349
1103	295-299	-0.05945821500281312
1103	300-301	-0.03964810698028032
1104	1	0.06086024308939386
1104	2	0.15296489247690914
1104	3	0.11542888812285668
1104	4	0.4260642233688614
1104	5	0.22604036089931867
1104	6	0.274048934156653
1104	7	0.2537209212310856
1104	8	0.14738108640997183
1104	9	0.1758805870259792
1104	10-14	0.05409960747832798
1104	15-19	0.12532497132421128
1104	20-24	0.079912621663901
1104	25-29	-0.038565668625111016
1104	30-34	0.06112181139272366
1104	35-39	0.04782149982715822
1104	40-44	0.13527426749968896
1104	45-49	0.06088352667257624
1104	50-54	-0.03407768844071768
1104	55-59	-0.05785096071371498
1104	60-64	-0.040898496599055534
1104	65-69	0.036376579002414644
1104	70-74	0.014222417511703611
1104	75-79	-0.12066176940120243
1104	80-84	-0.00621946871553547
1104	85-89	-0.010081832848165106
1104	90-94	-0.0593143191160479
1104	95-99	0.010474823201903405
1104	100-104	0.07297999357576401
1104	105-109	0.12226357983152525
1104	110-114	0.02041315714993175
1104	115-119	-0.17256396776717509
1104	120-124	0.020430113667941896
1104	125-129	-0.13339474145551833
1104	130-134	-0.027052018380139486
1104	135-139	-0.10424036791243907
1104	140-144	0.033096117175972495
1104	145-149	0.03233025595420003
1104	150-154	-0.01750693995104058
1104	155-159	-0.12379637592568571
1104	160-164	-0.06444294750554747
1104	165-169	0.012064492079417732
1104	170-174	-4.833712701994841E-4
1104	175-179	-0.050116918707857394
1104	180-184	-0.14868290857759803
1104	185-189	-0.16795792575747726
1104	190-194	-0.10282714535434678
1104	195-199	-0.04851464472582023
1104	200-204	-0.01307205643493603
1104	205-209	-0.2471262351023853
1104	210-214	0.06528901039315116
1104	215-219	-0.021004241454711803
1104	220-224	0.023224585267641373
1104	225-229	0.028410325563825722
1104	230-234	-0.15691601647335318
1104	235-239	0.12035764997632015
1104	240-244	-0.03483386948788336
1104	245-249	-0.3303894115097705
1104	250-254	-0.08845856089300241
1104	255-259	-0.056166597035371524
1104	260-264	-0.10387723693160922
1104	265-269	-0.07502642817261318
1104	270-274	0.06015582090470417
1104	275-279	-0.08445393847555493
1104	280-284	-0.2853334674725829
1104	285-289	-1.9947970540101778E-4
1104	290-294	0.0025121176632580955
1104	295-299	0.035912915234348475
1104	300-301	-0.07353292932948108
1201	1	0.16167865314393026
1201	2	0.31512992459893496
1201	3	-0.07302538634412059
1201	4	-0.14444115811338065
1201	5	-0.05602667439319475
1201	6	-0.10879100182684454
1201	7	-0.03555176329042098
1201	8	-0.20431527511641434
1201	9	-0.14544585922367048
1201	10-14	-0.04021096358834342
1201	15-19	-0.010872417955305025
1201	20-24	-0.01425838735453766
1201	25-29	0.027765745730057745
1201	30-34	-0.08661675248345091
1201	35-39	-0.04647390145165531
1201	40-44	0.04075435336068978
1201	45-49	-0.003742803528197669
1201	50-54	0.06171041996258708
1201	55-59	-0.13236495863546338
1201	60-64	0.12482536775269892
1201	65-69	-0.05800680704593475
1201	70-74	0.06758062929710462
1201	75-79	0.008609094745757773
1201	80-84	-0.03334608334983358
1201	85-89	-0.049817575143812576
1201	90-94	0.001592379103776409
1201	95-99	-0.03443988466591463
1201	100-104	-0.05312038491044291
1201	105-109	-0.05975263667169628
1201	110-114	-0.006176446994452078
1201	115-119	-0.12478216999561198
1201	120-124	-0.035722373039533295
1201	125-129	-0.02481243397910049
1201	130-134	-0.17534883044921656
1201	135-139	-0.0746774853493477
1201	140-144	-0.02741920051730773
1201	145-149	-0.02850969519908375
1201	150-154	-0.04224279602009062
1201	155-159	-0.18403256952251468
1201	160-164	-0.0892061091927232
1201	165-169	-0.05325054648977812
1201	170-174	-0.006675678670767127
1201	175-179	0.0061842319205567264
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2404	195-199	0.15677988306257618
2404	200-204	0.2710391121547673
2404	205-209	0.06688979392680494
2404	210-214	0.15446679166961985
2404	215-219	0.08162952746636165
2404	220-224	0.22562818992489753
2404	225-229	0.24701989349977538
2404	230-234	0.3062374588570904
2404	235-239	0.17183347523140924
2404	240-244	0.24527079152588271
2404	245-249	0.2212970852701801
2404	250-254	0.19923627278145517
2404	255-259	0.1473615887154338
2404	260-264	0.20707093487439465
2404	265-269	0.13357286858934003
2404	270-274	0.1808068783858019
2404	275-279	0.1830224265784608
2404	280-284	8.679225096415166E-4
2404	285-289	0.17565801337843112
2404	290-294	0.20472079493097795
2404	295-299	0.17610769267975712
2404	300-301	0.24693097570928302
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	48.0
22	77.0
23	129.0
24	304.0
25	283.0
26	282.0
27	348.0
28	402.0
29	452.0
30	509.0
31	550.0
32	706.0
33	824.0
34	1086.0
35	1477.0
36	2082.0
37	3139.0
38	6602.0
39	128762.0
40	13927.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.061729538201324714	0.006172953820132471	0.11111316876238449	99.82098433921615
2	0.012345983851453121	0.02037087335489765	0.16728808118718982	99.79999506160647
3	0.011111316876238448	0.1271628486947289	99.61542497700574	0.2463008574232856
4	0.08580405809984136	99.39690241177306	0.09753267035809304	0.41976085976900807
5	0.19691722686222585	0.13333580251486138	0.07654462736964264	99.59320234325327
6	99.78332932091335	0.05864306129125848	0.016666975314357672	0.1413606424810336
7	99.89444248967574	0.06605060587541745	0.02592640604455638	0.013580498404291437
8	99.73826675802638	0.11728612258251696	0.07777921813366914	0.06666790125743069
9	29.236961178293424	21.720772606900127	26.298635159910365	22.74363105489608
10-14	59.81493484447243	10.498589480052098	9.522645481089157	20.163830194386314
15-19	20.781619412705172	39.24455391149218	19.878022432514182	20.095804243288455
20-24	59.667524707247665	39.854071371692065	0.34494465946900255	0.13345926159126403
25-29	0.36926609752032447	0.666679012574307	59.482212633567286	39.481842256338076
30-34	0.480008889053501	59.56542405106268	0.31556139928517196	39.639005660598656
35-39	40.51210824891819	19.827898047494706	19.75999555547325	19.899998148113855
40-44	39.8092557269579	59.308011876763146	0.3721056562775854	0.5106267400013581
45-49	20.257782551528734	19.886911486015173	39.45900232720359	20.396303635252504
50-54	59.3473953221356	20.19321345457015	19.71900714210757	0.7403840811866886
55-59	20.200620999154307	39.42591529472768	0.40618036136471664	39.9672833447533
60-64	39.99925924554159	0.15691648610776743	0.34333969147576804	59.50048457687488
65-69	39.99333320987425	20.147533596301166	39.633573461236935	0.2255597325876405
70-74	40.12024914041618	59.62591899849997	0.20444823052278743	0.049383630561059766
75-79	59.40147039759995	19.807774218041075	19.950739828515342	0.8400155558436267
80-84	39.89320789891171	0.07284085507756316	39.71073538398859	20.32321586202214
85-89	20.316994698647626	0.6697679701524232	40.045457800296795	38.96777953090316
90-94	20.10000246919677	59.302204992715865	0.7885379885923108	19.80925454949505
95-99	19.896787575001852	0.36531766216449785	59.648880219264676	20.08901454356898
100-104	39.89839255290254	20.033951455591495	39.119978271068426	0.9476777204375416
105-109	40.66569544927035	39.61134842835626	19.51887700930887	0.2040791130645201
110-114	39.96320896812267	59.62628706881652	0.22914146028296997	0.18136250277784638
115-119	20.969159732339072	58.73787007086595	19.94802340798538	0.3449467888096002
120-124	58.71737573767254	20.48816020148646	20.46593743055384	0.3285266302871676
125-129	0.8648372365209468	39.77177186075195	39.21891802133142	20.14447288139569
130-134	39.15642643639987	0.8365855555651582	20.667712810154587	39.33927519788038
135-139	0.31076069080978036	19.864732056872786	78.55825312242267	1.2662541298947587
140-144	40.09247541499732	19.986912691602516	19.840729926106093	20.079881967294074
145-149	19.47786578100982	39.593182253115955	39.945551858459524	0.983400107414701
150-154	0.9109305492622302	20.13035517401885	58.47253071520024	20.48618356151868
155-159	20.580562239872386	78.8451969839704	0.19396522454160128	0.3802755516156155
160-164	20.34879342169433	19.906288776259824	19.476501200713635	40.268416601332206
165-169	39.44625185971714	20.34410168717243	20.152604838659894	20.057041614450544
170-174	0.622148690944335	39.34142863316192	20.015680270639002	40.020742405254744
175-179	39.9153018452098	0.49263212480013824	19.88307703704618	39.70898899294388
180-184	39.88048423638934	39.38995104545426	0.1516171050763333	20.57794761308006
185-189	40.095933624303186	0.3648440924271701	0.582762814299913	58.95645946896973
190-194	20.0096304024397	39.963577324106254	39.53625908251897	0.4905331909350751
195-199	20.53584546908085	39.45131399433288	20.08062375375802	19.932216782828252
200-204	79.12894244599875	0.37682036212782505	19.97728212757814	0.5169550642952829
205-209	20.55942760838833	39.82072635458401	0.48312518905838125	39.13672084796928
210-214	39.676393784686425	0.6811657725619957	58.826819436127586	0.8156210066239884
215-219	39.9199935797317	19.918758912752256	1.075765339193886	39.08548216832215
220-224	0.6886972411366344	20.20013951736868	78.55778550130566	0.5533777401890275
225-229	60.03173094137184	0.2445875286288406	19.681455919302167	20.042225610697155
230-234	20.209646453110437	59.33550223166056	19.92110478001321	0.533746535215789
235-239	59.18265045960478	20.09334082364634	0.6083204207745065	20.11568829597437
240-244	40.2021149845358	20.22767259101039	19.352170235883126	20.21804218857069
245-249	59.25339687752721	0.3369406186916234	20.246686462493905	20.162976041287266
250-254	0.8298196768876516	39.41661985221036	39.820355954490175	19.933204516411813
255-259	19.943822652435074	39.4351398569021	20.329162216721095	20.291875273941738
260-264	39.902337841925586	20.219029922154245	39.4761308006198	0.40250143530036364
265-269	20.629062826029248	59.299696889256545	19.54391401779155	0.5273262669226543
270-274	19.595399630834574	0.37027662713674553	39.538728416477866	40.495595325550816
275-279	39.81010821856075	0.7248729836344892	19.918758912752256	39.54625988505251
280-284	0.8520436825177329	39.93357491650564	0.36570835931278434	58.84867304166384
285-289	59.14030138220968	39.712569527184286	0.7554927247248236	0.3916363658812127
290-294	20.22164768981176	20.022866060691367	39.429879471660655	20.32560677783622
295-299	20.286018846413707	39.6577556487981	20.04491123361376	20.011314271174424
300-301	0.9651454549971431	98.39763495888505	0.5990236752540183	0.03819591086378655
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.3333333333333333
47	0.0
48	7.833333333333334
49	262.91666666666686
50	31994.166666666453
51	24112.249999999927
52	657.8333333333337
53	167.99999999999997
54	358.3333333333333
55	546.0833333333333
56	83.5
57	7.5
58	3.25
59	1.25
60	0.25
61	0.5
62	0.5
63	0.25
64	0.25
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.25
81	0.25
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	6.172953820132471E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	1.234666979448968E-4
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
85-89	1.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	3.0
130-134	3.0
135-139	1.0
140-144	0.0
145-149	0.0
150-154	1.0
155-159	1.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	2.0
295-299	861.0
300-302	161124.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.8741087797922182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.67457180500659	1.0808842139051957
2	10.210803689064559	0.38272313684821324
3	4.808959156785244	0.2703753773218023
4	2.404479578392622	0.18025025154786817
5	2.8326745718050064	0.26543701426569627
6	1.9433465085639	0.2185225652326895
7	1.844532279314888	0.24197978974919288
8	1.2845849802371543	0.1925961591881331
9	0.8893280632411068	0.15000277782921906
>10	13.405797101449277	5.899491965900603
>50	1.9762845849802373	2.3784391068970416
>100	0.461133069828722	1.637684648481145
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.2635046113306983	87.1016129928332
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	21834	13.478027370877237	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	19367	11.955159663450559	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	18949	11.69713019376902	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	18644	11.50885510225498	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	17923	11.063785131823428	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	16464	10.163151169466103	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	15800	9.753267035809305	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	12121	7.482237325382569	No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	250	0.1543238455033118	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	248	0.15308925473928528	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	235	0.14506441477311308	No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	219	0.13518768866090114	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	215	0.13271850713284813	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	210	0.1296320302227819	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	203	0.12531096254868918	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	198	0.12222448563862294	No Hit
CCTACGGGCGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	178	0.109878577998358	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	173	0.10679210108829175	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
