##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	JAFS2_cleaned_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	83508
Sequences flagged as poor quality	0
Sequence length	76-301
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.66465488336447	40.0	40.0	40.0	40.0	40.0
2	39.10497197873258	40.0	40.0	40.0	40.0	40.0
3	39.32471140489534	40.0	40.0	40.0	40.0	40.0
4	39.03285912726924	40.0	40.0	40.0	40.0	40.0
5	39.31216170905782	40.0	40.0	40.0	40.0	40.0
6	39.35278057192125	40.0	40.0	40.0	40.0	40.0
7	39.4705177947023	40.0	40.0	40.0	40.0	40.0
8	39.50864587823921	40.0	40.0	40.0	40.0	40.0
9	38.99750922067347	40.0	40.0	40.0	40.0	40.0
10-14	39.62690999664703	40.0	40.0	40.0	40.0	40.0
15-19	39.522431383819516	40.0	40.0	40.0	40.0	40.0
20-24	39.49237917325287	40.0	40.0	40.0	40.0	40.0
25-29	39.65374335393016	40.0	40.0	40.0	40.0	40.0
30-34	39.503683479427124	40.0	40.0	40.0	40.0	40.0
35-39	39.64958566843895	40.0	40.0	40.0	40.0	40.0
40-44	39.58248790535039	40.0	40.0	40.0	40.0	40.0
45-49	39.504957608851846	40.0	40.0	40.0	40.0	40.0
50-54	39.56810844469991	40.0	40.0	40.0	40.0	40.0
55-59	39.43060784595488	40.0	40.0	40.0	40.0	40.0
60-64	39.417588734013506	40.0	40.0	40.0	40.0	40.0
65-69	39.535335536715046	40.0	40.0	40.0	40.0	40.0
70-74	39.431776596254245	40.0	40.0	40.0	40.0	40.0
75-79	39.418226610312296	40.0	40.0	40.0	40.0	40.0
80-84	39.49655764974231	40.0	40.0	40.0	40.0	40.0
85-89	39.62221636768616	40.0	40.0	40.0	40.0	40.0
90-94	39.48134363211784	40.0	40.0	40.0	40.0	40.0
95-99	39.317425303874025	40.0	40.0	40.0	36.8	40.0
100-104	39.28829171905873	40.0	40.0	40.0	40.0	40.0
105-109	39.41024848811448	40.0	40.0	40.0	40.0	40.0
110-114	39.40652176516376	40.0	40.0	40.0	40.0	40.0
115-119	39.36433985988863	40.0	40.0	40.0	40.0	40.0
120-124	39.26338856946069	40.0	40.0	40.0	40.0	40.0
125-129	39.419735581529025	40.0	40.0	40.0	40.0	40.0
130-134	39.419611036597054	40.0	40.0	40.0	40.0	40.0
135-139	39.2220061314428	40.0	40.0	40.0	40.0	40.0
140-144	39.48654839378427	40.0	40.0	40.0	40.0	40.0
145-149	39.10402146056382	40.0	40.0	40.0	36.8	40.0
150-154	39.34144080483861	40.0	40.0	40.0	40.0	40.0
155-159	39.16408886759687	40.0	40.0	40.0	40.0	40.0
160-164	39.39631355170968	40.0	40.0	40.0	40.0	40.0
165-169	39.34569495179352	40.0	40.0	40.0	40.0	40.0
170-174	39.064614647583696	40.0	40.0	40.0	40.0	40.0
175-179	39.350332355230854	40.0	40.0	40.0	40.0	40.0
180-184	39.39521168932272	40.0	40.0	40.0	40.0	40.0
185-189	39.286343375495726	40.0	40.0	40.0	40.0	40.0
190-194	39.409825855750114	40.0	40.0	40.0	40.0	40.0
195-199	39.3196987719014	40.0	40.0	40.0	40.0	40.0
200-204	39.52031427784365	40.0	40.0	40.0	40.0	40.0
205-209	39.30003233804032	40.0	40.0	40.0	40.0	40.0
210-214	39.39866575641072	40.0	40.0	40.0	40.0	40.0
215-219	39.39292635310745	40.0	40.0	40.0	40.0	40.0
220-224	39.31237349238858	40.0	40.0	40.0	40.0	40.0
225-229	39.41146682955457	40.0	40.0	40.0	40.0	40.0
230-234	39.22367623633119	40.0	40.0	40.0	40.0	40.0
235-239	39.40416561867462	40.0	40.0	40.0	40.0	40.0
240-244	39.3265926484855	40.0	40.0	40.0	40.0	40.0
245-249	39.202098379504875	40.0	40.0	40.0	40.0	40.0
250-254	39.31878361060208	40.0	40.0	40.0	40.0	40.0
255-259	39.18986262321392	40.0	40.0	40.0	40.0	40.0
260-264	39.16800689878193	40.0	40.0	40.0	40.0	40.0
265-269	39.23213682584169	40.0	40.0	40.0	40.0	40.0
270-274	37.92713640664487	40.0	36.8	40.0	33.6	40.0
275-279	39.198795240776874	40.0	40.0	40.0	40.0	40.0
280-284	38.30332966032674	40.0	40.0	40.0	36.8	40.0
285-289	39.125185646529005	40.0	40.0	40.0	40.0	40.0
290-294	39.18814207696086	40.0	40.0	40.0	40.0	40.0
295-299	39.10276769317362	40.0	40.0	40.0	40.0	40.0
300-301	38.25386592414034	40.0	40.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22617418200848505
1101	2	0.24163896298219356
1101	3	0.09112064858206992
1101	4	-0.272055463157173
1101	5	0.11421014377665273
1101	6	-0.08757047544671792
1101	7	-0.1199052365294051
1101	8	-0.17467742996342395
1101	9	0.6010095151919472
1101	10-14	-0.01753478795031782
1101	15-19	-0.25390063866326074
1101	20-24	0.0020414336396257227
1101	25-29	-0.15709288453801662
1101	30-34	-0.19491368428585076
1101	35-39	-0.0589816119025528
1101	40-44	-0.21216053937687462
1101	45-49	-0.037796094190525764
1101	50-54	-0.005587762992753653
1101	55-59	0.00343278641867073
1101	60-64	-0.09543394852444465
1101	65-69	0.007177732864775521
1101	70-74	-0.027498113996031748
1101	75-79	-0.10152612991164034
1101	80-84	-0.062468469465819965
1101	85-89	-0.097655439101068
1101	90-94	-0.16462015641461392
1101	95-99	-0.05476935330873545
1101	100-104	-0.10984352692162958
1101	105-109	-0.2006222350426441
1101	110-114	-0.09631086043044235
1101	115-119	-0.19403469891064162
1101	120-124	-0.17500014094874672
1101	125-129	-0.2334118028297496
1101	130-134	-0.21167351984293958
1101	135-139	-0.07294216494339878
1101	140-144	-0.09101490032268345
1101	145-149	-0.07969865939949017
1101	150-154	-0.07190367235175898
1101	155-159	-0.1280109908204139
1101	160-164	-0.1334059107991834
1101	165-169	0.017206554328403456
1101	170-174	0.09914990748750085
1101	175-179	-0.006709629648455007
1101	180-184	-0.16145819101910774
1101	185-189	-0.13335471545601507
1101	190-194	-0.07974436765465498
1101	195-199	-0.052859680896702343
1101	200-204	-0.11374639456523283
1101	205-209	-0.13300336595153794
1101	210-214	-0.022731322060387527
1101	215-219	-0.07803667180313312
1101	220-224	-0.2738402971457461
1101	225-229	-0.16524993538799038
1101	230-234	-0.12110700856349865
1101	235-239	-0.11645894249453193
1101	240-244	-0.12643264796949438
1101	245-249	-0.10570908538528556
1101	250-254	-0.14001398478748683
1101	255-259	-0.20243190240972098
1101	260-264	-0.35992240869049397
1101	265-269	-0.19378604401976673
1101	270-274	0.01748037620805576
1101	275-279	-0.14996572013898657
1101	280-284	-0.2306213731296083
1101	285-289	-0.20609868828326228
1101	290-294	-0.3418813861050509
1101	295-299	-0.3009215170700017
1101	300-301	-0.26691047956012426
1102	1	0.28543344126774883
1102	2	-0.06947214812891644
1102	3	0.4763058337672561
1102	4	0.1279445368428327
1102	5	0.41050644007295034
1102	6	0.38650359862735684
1102	7	0.2060206893965244
1102	8	0.22532257003657463
1102	9	-0.46565715147471565
1102	10-14	0.16616891575338855
1102	15-19	0.17572899096636974
1102	20-24	-0.039440047841857506
1102	25-29	0.04142563398050214
1102	30-34	0.11323446386230529
1102	35-39	2.7764735671098606E-4
1102	40-44	-0.028456835673168257
1102	45-49	0.0451668687724478
1102	50-54	-0.10040257780756434
1102	55-59	-0.06767832469243729
1102	60-64	0.014195681105178437
1102	65-69	0.0012518069388463005
1102	70-74	0.11176114526323744
1102	75-79	0.06439979601428547
1102	80-84	-0.02987587687323412
1102	85-89	0.15123344978781716
1102	90-94	0.03686132506687301
1102	95-99	-0.10217676071614079
1102	100-104	0.026452769374671448
1102	105-109	0.036414801994403945
1102	110-114	-0.010384934504521937
1102	115-119	0.09337270849677282
1102	120-124	0.017592451643849927
1102	125-129	0.04806967865172851
1102	130-134	-0.07537722354663856
1102	135-139	0.0515022795010438
1102	140-144	-0.06731119661898077
1102	145-149	-0.05895791865873434
1102	150-154	-0.2585703390184264
1102	155-159	-0.10134432415375016
1102	160-164	-0.1541466515399179
1102	165-169	-0.07760826048640723
1102	170-174	0.09914990748750085
1102	175-179	0.06736444442562117
1102	180-184	-0.05479152435244572
1102	185-189	0.02960824750694968
1102	190-194	-0.11529992321020899
1102	195-199	-0.17730412534114492
1102	200-204	0.010698049879209748
1102	205-209	-0.002632995581166142
1102	210-214	0.15504645571738962
1102	215-219	-0.012851486617947216
1102	220-224	0.03134488803943469
1102	225-229	0.056972286834216845
1102	230-234	-0.06184774930423487
1102	235-239	-0.14312560916118855
1102	240-244	-0.20346968500652451
1102	245-249	-0.10867204834825372
1102	250-254	-0.11927324404673811
1102	255-259	-0.1283578283356448
1102	260-264	-0.03399648276457157
1102	265-269	-0.15526752550125167
1102	270-274	0.011554450282126538
1102	275-279	-0.25366942384268754
1102	280-284	0.027156404648167154
1102	285-289	-0.3275801697647438
1102	290-294	-0.2618813861050526
1102	295-299	-0.31646601679360487
1102	300-301	-0.15210771500573372
1103	1	0.050575053468179476
1103	2	0.07028820045496076
1103	3	0.37510757668664496
1103	4	0.22238898128727413
1103	5	0.23577877122762914
1103	6	0.15327919775589294
1103	7	0.04687036259913668
1103	8	0.006912983979930232
1103	9	-0.20476390528735067
1103	10-14	0.05307523383618218
1103	15-19	0.048735526914086336
1103	20-24	0.023675420567734307
1103	25-29	-0.00907545534412435
1103	30-34	-0.016242660320699542
1103	35-39	-0.01170492344940044
1103	40-44	0.06620547369502106
1103	45-49	-0.0013037194628466864
1103	50-54	0.032930755525775623
1103	55-59	0.12835653369536715
1103	60-64	-0.08445355636758478
1103	65-69	0.10785311412838894
1103	70-74	0.1153994894898176
1103	75-79	0.012656876624312474
1103	80-84	-0.09623753264665424
1103	85-89	0.05317244760917106
1103	90-94	-0.006297716327466674
1103	95-99	-0.0960765428512147
1103	100-104	-0.10058426766237005
1103	105-109	0.11726447519701111
1103	110-114	2.033007896002914E-4
1103	115-119	0.0772942771242171
1103	120-124	-0.1737800973757544
1103	125-129	0.017111072987241016
1103	130-134	0.035712101072093105
1103	135-139	-0.1601317074270554
1103	140-144	-0.21258352777366696
1103	145-149	0.010301340600513242
1103	150-154	-0.05133722355000003
1103	155-159	-0.1205817969206322
1103	160-164	0.028554873514544
1103	165-169	-0.21590891408117585
1103	170-174	-0.02054508161926094
1103	175-179	-0.13670962964845756
1103	180-184	-0.12901810387314327
1103	185-189	-0.2473198570682129
1103	190-194	-0.19506027179409102
1103	195-199	-0.1351908355808007
1103	200-204	-0.08531502201621777
1103	205-209	-0.09128223305393135
1103	210-214	-0.1323827381823932
1103	215-219	-0.21367937332819054
1103	220-224	-0.2456485760128544
1103	225-229	-0.05365952144464359
1103	230-234	-0.1140699715264617
1103	235-239	-0.2550210339977923
1103	240-244	-0.18780519698909615
1103	245-249	-0.2634215036859402
1103	250-254	0.03983350976589861
1103	255-259	-0.11232296994784718
1103	260-264	-0.1271773106512839
1103	265-269	-0.2129145843247784
1103	270-274	-0.1730860725936907
1103	275-279	-0.04271081817821454
1103	280-284	-0.13691766942590533
1103	285-289	-0.2565562046231378
1103	290-294	-0.17708835778260834
1103	295-299	-0.14237015622715177
1103	300-301	-0.38112118056438504
1104	1	-0.14572875541168884
1104	2	0.07969771138576931
1104	3	-0.0069636426056689515
1104	4	0.05284875140221601
1104	5	0.03451665718661445
1104	6	0.054958260185465235
1104	7	0.009852107021046663
1104	8	-0.12205929458666986
1104	9	-0.07229827535721967
1104	10-14	0.02374235125785873
1104	15-19	0.07549910590889652
1104	20-24	0.05690733402276038
1104	25-29	0.10967595326530244
1104	30-34	-0.12707204954766382
1104	35-39	0.005897059872665977
1104	40-44	-0.044804217537794955
1104	45-49	0.1297645699218677
1104	50-54	0.04365872487443312
1104	55-59	-0.019964403875064818
1104	60-64	-0.10708145810299641
1104	65-69	-0.049629419114793905
1104	70-74	0.09163343134241586
1104	75-79	-0.05003187703807299
1104	80-84	-0.02824113868677358
1104	85-89	0.126814548127534
1104	90-94	0.05841943490084134
1104	95-99	-0.11545900848114599
1104	100-104	0.09041190092001017
1104	105-109	0.11702782881432228
1104	110-114	0.0686955252655963
1104	115-119	0.12433056290289812
1104	120-124	-0.08876770161285208
1104	125-129	0.17486405923921922
1104	130-134	0.1607402732605081
1104	135-139	0.05701952088035256
1104	140-144	0.026992762512563218
1104	145-149	0.03463084251622206
1104	150-154	-0.08406203761356323
1104	155-159	-0.021855179837018568
1104	160-164	0.11088527694028016
1104	165-169	0.09741089660170843
1104	170-174	-0.012318802601903656
1104	175-179	0.06654707533238025
1104	180-184	-0.1588017414661067
1104	185-189	-0.07552585210990514
1104	190-194	-0.018646027935623977
1104	195-199	-0.07135265663105628
1104	200-204	-0.06623681602117415
1104	205-209	-0.15088331486597184
1104	210-214	0.238622445500269
1104	215-219	-0.021229519823570797
1104	220-224	0.05218779991682965
1104	225-229	0.03786628427994998
1104	230-234	-0.04376345811649429
1104	235-239	-0.035232888854679345
1104	240-244	0.15372060873549032
1104	245-249	0.054597428024671046
1104	250-254	0.04798090665568111
1104	255-259	-0.15911134046847053
1104	260-264	-0.032259573441450584
1104	265-269	-0.2036966442751975
1104	270-274	-0.37618501331940735
1104	275-279	-0.055253076460822115
1104	280-284	-0.03404410620751719
1104	285-289	-0.19015999096525604
1104	290-294	-0.14152378712675784
1104	295-299	-0.2605784050439155
1104	300-301	-0.40753230439247545
1201	1	-0.06556370972940329
1201	2	0.17603049737373055
1201	3	0.08330624076766213
1201	4	-0.09720808830979877
1201	5	-0.04110121153470203
1201	6	0.03794845007220715
1201	7	0.0341037174795531
1201	8	-0.09376574905174095
1201	9	0.2099635241459623
1201	10-14	0.019127808712283922
1201	15-19	-0.01113303589565362
1201	20-24	0.09005120164939484
1201	25-29	0.20038371293858148
1201	30-34	0.09994997057781774
1201	35-39	0.04830369538275647
1201	40-44	0.19946339224706122
1201	45-49	0.035789579395157034
1201	50-54	0.15161207420709388
1201	55-59	0.08610270908859974
1201	60-64	0.038192425101925664
1201	65-69	0.1415204272074746
1201	70-74	0.08496423846633405
1201	75-79	0.18487191648640788
1201	80-84	0.01746641046905495
1201	85-89	0.12482726338163275
1201	90-94	0.19800092620647547
1201	95-99	0.2644492059098198
1201	100-104	0.09300547592737729
1201	105-109	0.004864130443728243
1201	110-114	0.09921213509255011
1201	115-119	0.24077197589603827
1201	120-124	0.14916754321894388
1201	125-129	0.23916450974656556
1201	130-134	0.09064638247696877
1201	135-139	0.11030569830446524
1201	140-144	0.1566041472963633
1201	145-149	0.23942221972139777
1201	150-154	0.21231285186477322
1201	155-159	0.2142275114180876
1201	160-164	0.15699701960375023
1201	165-169	0.0629534000752443
1201	170-174	-0.08120011286251838
1201	175-179	0.0158544729156489
1201	180-184	0.06138267182175383
1201	185-189	0.04650690440561078
1201	190-194	0.08637691846663387
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2404	250-254	-0.21308555259589923
2404	255-259	-0.058895283083629124
2404	260-264	-0.07200260919174895
2404	265-269	-0.06744797662906876
2404	270-274	-0.1491807209793592
2404	275-279	-0.12269754971292457
2404	280-284	-0.019248496493574407
2404	285-289	-0.16606527140775285
2404	290-294	7.975334159766589E-4
2404	295-299	0.09139808791260862
2404	300-301	-0.007817850904437762
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	12.0
22	21.0
23	53.0
24	109.0
25	138.0
26	150.0
27	169.0
28	160.0
29	219.0
30	251.0
31	261.0
32	387.0
33	433.0
34	592.0
35	719.0
36	1006.0
37	1562.0
38	3333.0
39	66539.0
40	7389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.050294582554964794	0.005987450304162476	0.08861426450160463	99.85510370263927
2	0.008382530805800712	0.017962566012430098	0.1508855545044128	99.82276934867735
3	0.0035924701824974854	0.13531637687407194	99.65632035254107	0.20477080040235668
4	0.08023183407577718	99.44556210183455	0.08621928437993964	0.3879867797097284
5	0.1748335488815443	0.10537912535325956	0.08142932413660967	99.63835800162859
6	99.8119940604493	0.06705944340661972	0.013172390669157446	0.10777410547492457
7	99.89222589452508	0.058677012980792254	0.03712219188580735	0.011974900608324951
8	99.77846433874599	0.08981175456243713	0.08262681419744217	0.04909709249413229
9	30.36954543277291	20.333381232935764	26.815394932222063	22.481678402069264
10-14	59.82277147099679	10.965895483067492	9.050629879771996	20.16070316616372
15-19	38.22627772189491	21.445370503424822	19.91473870766873	20.413613067011543
20-24	59.81965799683863	39.77463237055133	0.3484696077022561	0.05724002490779327
25-29	16.850361641998372	16.9487953249988	60.58820711788092	5.6126359151219045
30-34	1.9569382574124636	58.322795420798016	0.3297887627532691	39.390477559036256
35-39	59.62853858312977	2.2910379843847295	19.752838051444176	18.327585381041338
40-44	38.16041576854912	45.16860660056521	0.16333764429755232	16.50763998658811
45-49	20.155434209896057	19.908272261340233	22.078603247593044	37.85769028117066
50-54	42.72141591224793	20.154955213871727	18.487809551180725	18.63581932269962
55-59	20.304880969487954	25.195909373952198	15.793217416295443	38.70599224026441
60-64	39.97820568089285	0.10082866312209608	0.5295301049001293	59.391435551084925
65-69	26.278200890932606	20.920390860755855	51.882693873640854	0.9187143746706903
70-74	40.4754035541505	57.66968434161996	1.7433060305599462	0.11160607366958854
75-79	41.707441496202726	19.930449277549055	5.636147215695854	32.72596201055236
80-84	39.96187127724036	1.429108597404277	38.46091207162068	20.148108053734674
85-89	35.41733528129715	2.8603932651546	55.811558450889756	5.9107130026584915
90-94	19.433087839051556	58.485120651458	16.06155320040716	6.020238309083288
95-99	4.308963535117657	14.51457996527154	60.65481108915633	20.521645410454465
100-104	35.926950482007065	20.35997844440453	8.23351895096102	35.47955212262739
105-109	70.63912340578409	26.95623016585833	2.0702951919046764	0.334351236452907
110-114	54.494461409496445	43.11646009221005	0.8030656846895394	1.5860128136039757
115-119	36.65265552960901	42.00514939225196	18.709777857613314	2.632417220525717
120-124	26.516926054195945	34.63170802975652	34.922471151220776	3.928894764826764
125-129	5.766909369611037	49.94874497030082	22.585504885993483	21.698840774094656
130-134	20.353755508718148	14.534154052500478	46.46340295075685	18.648687488024525
135-139	3.8999329373443183	23.014466372868366	36.861707223606054	36.22389346618126
140-144	39.73586387936155	17.48835961601104	19.882063270037747	22.89371323458966
145-149	2.375511963785298	26.696366554094514	37.23024598213217	33.69787549998802
150-154	35.77124378705312	16.976106353673874	10.477992694173304	36.774657165099704
155-159	21.491586322534285	70.95514701479131	0.2172585184741601	7.336008144200251
160-164	23.654829630516797	17.773998443020538	1.9517336367447151	56.61943828971795
165-169	38.82723516378226	22.366369243667286	20.784478112461823	18.02191748008863
170-174	3.933888256781843	25.10114378106473	20.266842325887776	50.698125636265644
175-179	44.70806635127852	4.041439607162105	18.141924666147673	33.1085693754117
180-184	38.285885382358224	38.76495598538835	1.6441703095993772	21.30498832265405
185-189	39.259159895753484	1.3778169553886248	7.915261382799326	51.44776176605856
190-194	22.04853043332455	35.1606103432582	37.051524660454646	5.739334562962608
195-199	33.883076068105815	41.73253997911217	6.90328360497092	17.48110034781109
200-204	74.47043464721594	1.5543818044626496	21.424311020085515	2.5508725282359
205-209	22.047117722443797	39.25957864731175	3.67647587222881	35.01682775801564
210-214	27.028134095073835	34.40384223827147	37.02058855233373	1.547435114320961
215-219	38.90146479345574	19.161127280131268	19.865377935874864	22.072029990538127
220-224	11.873330698381901	32.3389984789144	39.388691267531414	16.398979555172293
225-229	54.58685159234906	2.0895164864120344	24.389110464350306	18.9345214568886
230-234	20.22732444636077	56.044698357946174	21.900518606350232	1.8274585893428192
235-239	40.157378462865154	19.48905896302684	6.390236307235337	33.96332626687267
240-244	36.840693231767936	23.40651312086043	6.817817062508234	32.9349765848634
245-249	50.91732241026193	7.505060304456661	21.359634939456	20.2179823458254
250-254	22.84215443210808	6.763680787610937	63.81229564155079	6.581869138730193
255-259	16.70391529828848	40.005988525984215	20.432611117099636	22.85748505862767
260-264	37.77107062867546	32.76562107002982	23.996981782903955	5.466326518390764
265-269	22.82394931311607	56.96142191560969	17.116884050159893	3.0977447211143447
270-274	6.9730396560190675	4.046327237013882	23.166732540452493	65.81390056651456
275-279	49.9284963146258	11.40513724635355	17.38821111433164	21.278155324689013
280-284	3.1890480525080247	36.335696833229534	8.006994682125233	52.468260432137214
285-289	51.48589086379533	25.57514492406458	18.92588511474153	4.013079097398553
290-294	26.029818997154162	20.0681755028123	22.05163707444209	31.850368425591448
295-299	24.414747780738924	24.009960312134517	32.3029507065207	19.272341200605865
300-301	10.9620699226434	53.049008604564165	35.97262952101662	0.016291951775822745
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.3333333333333333
46	2.333333333333333
47	2.083333333333333
48	7.166666666666666
49	104.41666666666666
50	2858.583333333334
51	1534.7499999999995
52	92.91666666666666
53	210.91666666666666
54	8325.833333333328
55	13791.000000000044
56	1632.3333333333303
57	153.74999999999997
58	23.16666666666666
59	11.083333333333334
60	5.833333333333333
61	3.3333333333333335
62	1.0833333333333333
63	0.8333333333333333
64	2.0
65	0.5
66	0.5
67	0.0
68	0.0
69	0.25
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0011974900608324951
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	1.0
80-84	1.0
85-89	1.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	1.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	2.0
145-149	7.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	1.0
190-194	0.0
195-199	1.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	1.0
280-284	0.0
285-289	0.0
290-294	2.0
295-299	450.0
300-302	83040.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.2882119078411645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.3077352694778	2.50035924701825
2	15.275062831611283	1.3100541265507497
3	6.22731080703714	0.8011208506969393
4	4.077073443172298	0.6993341955261771
5	2.960067020385367	0.6346697322412224
6	2.317788327282882	0.5963500502945825
7	1.1449315833566043	0.3436796474589261
8	1.0611561016475846	0.3640369784930785
9	0.6702038536721586	0.2586578531398189
>10	6.506562412733873	4.96239881208986
>50	0.1954761239877129	0.5939550701729176
>100	0.5585032113934655	7.456770608803947
>500	0.4188774085450991	13.411888681323944
>1k	0.08377548170901983	6.701154380418642
>5k	0.1954761239877129	59.36556976577094
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9290	11.12468266513388	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	7609	9.111701872874454	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	7359	8.812329357666332	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	6673	7.99085117593524	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	6628	7.936964123197778	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	6134	7.345404033146524	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	5882	7.043636537816736	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	3384	4.052306365857163	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1207	1.4453705034248217	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1005	1.2034775111366576	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	949	1.136418067730038	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	923	1.105283326148393	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	923	1.105283326148393	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	865	1.0358289026201082	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	816	0.9771518896393159	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	759	0.9088949561718638	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	758	0.9076974661110312	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	723	0.865785313981894	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	718	0.8597978636777314	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	701	0.8394405326435791	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	661	0.7915409302102793	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	658	0.7879484600277817	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	616	0.737653877472817	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	616	0.737653877472817	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	514	0.6155098912679025	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	487	0.5831776596254251	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	465	0.5568328782871103	No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	441	0.5280931168271303	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	435	0.5209081764621354	No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	430	0.5149207261579729	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	367	0.4394788523255257	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	349	0.4179240312305408	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	332	0.39756670019638835	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	327	0.3915792498922259	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	297	0.35565454806725105	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	296	0.3544570580064185	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	284	0.34008717727642856	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	281	0.33649470709393114	No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	271	0.3245198064856062	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	266	0.3185323561814437	No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	244	0.2921875748431288	No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	202	0.24189299228816402	No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	180	0.21554821094984913	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	140	0.1676486085165493	No Hit
CCTACGGGAGGCAGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	133	0.15926617809072185	No Hit
CCTACGGGTGGCAGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	99	0.11855151602241701	No Hit
CCTACGGGGGGCAGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	92	0.11016908559658954	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
