##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	JAFS1_cleaned_R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	131141
Sequences flagged as poor quality	0
Sequence length	38-301
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.14934307348579	40.0	40.0	40.0	40.0	40.0
2	38.72851358461503	40.0	40.0	40.0	40.0	40.0
3	39.3400385844244	40.0	40.0	40.0	40.0	40.0
4	39.344766320220224	40.0	40.0	40.0	40.0	40.0
5	38.71073119771849	40.0	40.0	40.0	40.0	40.0
6	38.14901518213221	40.0	40.0	40.0	24.0	40.0
7	38.746967004979375	40.0	40.0	40.0	40.0	40.0
8	38.92741400477349	40.0	40.0	40.0	40.0	40.0
9	39.36016958845823	40.0	40.0	40.0	40.0	40.0
10-14	39.2709343378501	40.0	40.0	40.0	40.0	40.0
15-19	39.13513546488131	40.0	40.0	40.0	40.0	40.0
20-24	39.14390770239665	40.0	40.0	40.0	40.0	40.0
25-29	39.32074942237744	40.0	40.0	40.0	40.0	40.0
30-34	39.09121937456631	40.0	40.0	40.0	40.0	40.0
35-39	39.440827079520375	40.0	40.0	40.0	40.0	40.0
40-44	39.375586396217784	40.0	40.0	40.0	40.0	40.0
45-49	39.188946164404456	40.0	40.0	40.0	40.0	40.0
50-54	39.398435876928616	40.0	40.0	40.0	40.0	40.0
55-59	39.1487871647641	40.0	40.0	40.0	40.0	40.0
60-64	39.00387832757609	40.0	40.0	40.0	40.0	40.0
65-69	39.16768619556349	40.0	40.0	40.0	40.0	40.0
70-74	39.039535149726625	40.0	40.0	40.0	40.0	40.0
75-79	39.25281113932545	40.0	40.0	40.0	40.0	40.0
80-84	38.83529384851188	40.0	40.0	40.0	36.8	40.0
85-89	39.115700156940775	40.0	40.0	40.0	40.0	40.0
90-94	39.02759446372058	40.0	40.0	40.0	36.8	40.0
95-99	37.74729858542723	40.0	36.8	40.0	36.8	40.0
100-104	38.616973348076414	40.0	40.0	40.0	36.8	40.0
105-109	38.937375986578715	40.0	40.0	40.0	40.0	40.0
110-114	39.242740687078204	40.0	40.0	40.0	40.0	40.0
115-119	39.15357146099264	40.0	40.0	40.0	40.0	40.0
120-124	38.879141024761125	40.0	40.0	40.0	40.0	40.0
125-129	39.11093622505395	40.0	40.0	40.0	40.0	40.0
130-134	39.22114912422693	40.0	40.0	40.0	40.0	40.0
135-139	38.29570814141476	40.0	40.0	40.0	33.6	40.0
140-144	38.378562059604064	40.0	40.0	40.0	36.8	40.0
145-149	38.690140393957186	40.0	40.0	40.0	36.8	40.0
150-154	38.97019316561301	40.0	40.0	40.0	36.8	40.0
155-159	39.18573030023411	40.0	40.0	40.0	40.0	40.0
160-164	38.62779053008061	40.0	40.0	40.0	36.8	40.0
165-169	39.060804844010946	40.0	40.0	40.0	40.0	40.0
170-174	38.94606157201577	40.0	40.0	40.0	40.0	40.0
175-179	39.09446278911928	40.0	40.0	40.0	40.0	40.0
180-184	38.88059421494536	40.0	40.0	40.0	40.0	40.0
185-189	39.1529890715489	40.0	40.0	40.0	40.0	40.0
190-194	38.19271257530694	40.0	36.8	40.0	36.8	40.0
195-199	39.09554182871959	40.0	40.0	40.0	40.0	40.0
200-204	38.72797376649127	40.0	40.0	40.0	40.0	40.0
205-209	38.82575764508503	40.0	40.0	40.0	40.0	40.0
210-214	38.04618927781591	40.0	40.0	40.0	36.8	40.0
215-219	38.696353237245475	40.0	40.0	40.0	40.0	40.0
220-224	38.67274307938687	40.0	40.0	40.0	36.8	40.0
225-229	38.45219858156029	40.0	40.0	40.0	33.6	40.0
230-234	38.92003355448791	40.0	40.0	40.0	40.0	40.0
235-239	38.39552200106765	40.0	40.0	40.0	33.6	40.0
240-244	38.71202013269275	40.0	40.0	40.0	40.0	40.0
245-249	37.29572180279112	40.0	36.8	40.0	31.2	40.0
250-254	38.60798291771524	40.0	40.0	40.0	40.0	40.0
255-259	38.68332189430336	40.0	40.0	40.0	40.0	40.0
260-264	38.21853122855182	40.0	40.0	40.0	33.6	40.0
265-269	37.759255700449934	40.0	40.0	40.0	30.4	40.0
270-274	38.65480973080149	40.0	40.0	40.0	40.0	40.0
275-279	37.90596950879197	40.0	40.0	40.0	33.6	40.0
280-284	38.48757244829479	40.0	40.0	40.0	36.8	40.0
285-289	38.584015618327136	40.0	40.0	40.0	40.0	40.0
290-294	38.11793057165518	40.0	40.0	40.0	33.6	40.0
295-299	38.43081372063441	40.0	40.0	40.0	36.8	40.0
300-301	34.805442844736675	40.0	32.0	40.0	18.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.28876247577613867
1101	2	0.22535311909912537
1101	3	-0.1932479271648191
1101	4	-0.16965242337622044
1101	5	-0.3759112232362867
1101	6	0.28589063397573256
1101	7	-0.09970051305281658
1101	8	-0.23379034043262692
1101	9	-0.09438003497599112
1101	10-14	-0.1794949000713899
1101	15-19	-0.2803756636306929
1101	20-24	-0.4869585471136517
1101	25-29	-0.24302571625306513
1101	30-34	-0.12438180796471698
1101	35-39	-0.2953310001660796
1101	40-44	-0.1541369612889838
1101	45-49	-0.10889832103998742
1101	50-54	-0.29052486233123886
1101	55-59	-0.17803677241571592
1101	60-64	-0.23250504613357492
1101	65-69	-0.29839686640645624
1101	70-74	-0.31730988472561705
1101	75-79	-0.2545129293369399
1101	80-84	-0.13075215663805295
1101	85-89	-0.25658936000260724
1101	90-94	-0.2538982955387894
1101	95-99	-0.07865538232643843
1101	100-104	-0.03566373931032274
1101	105-109	-0.26463198967386603
1101	110-114	-0.26288634140815503
1101	115-119	-0.23633747283715678
1101	120-124	-0.27278028863859305
1101	125-129	-0.16588765426717345
1101	130-134	-0.28282719759071995
1101	135-139	-0.3233753735473073
1101	140-144	-0.07817410087426424
1101	145-149	-0.44786218645167253
1101	150-154	-0.1896106462362539
1101	155-159	-0.2362142394606792
1101	160-164	-0.5168254896778919
1101	165-169	-0.2050830549124072
1101	170-174	-0.41515275108159955
1101	175-179	-0.22489038286517626
1101	180-184	-0.29226735408506244
1101	185-189	-0.15860605404144934
1101	190-194	-0.08880013839541334
1101	195-199	-0.19349509837719125
1101	200-204	-0.13772017941659698
1101	205-209	-0.14861595973482622
1101	210-214	-0.10017255323942464
1101	215-219	-0.3972511502903515
1101	220-224	-0.11092654177669914
1101	225-229	-0.42428826543096676
1101	230-234	-0.19051758162174792
1101	235-239	-0.07213312586131337
1101	240-244	-0.48563610315873973
1101	245-249	0.391781543912721
1101	250-254	-0.22488838954680546
1101	255-259	-0.24716083271877665
1101	260-264	-0.4229804742000738
1101	265-269	-0.3089424386557482
1101	270-274	-0.4506655120153269
1101	275-279	-0.4710217937787817
1101	280-284	-0.4550648081943294
1101	285-289	-0.5276753296512098
1101	290-294	-0.7636842121876057
1101	295-299	-0.5891695511567789
1101	300-301	-1.5843962983729512
1102	1	-0.5462226253568616
1102	2	0.030142723994494247
1102	3	0.14308205697055598
1102	4	-0.05557823738242007
1102	5	0.09262394334223956
1102	6	-0.23205149792726587
1102	7	0.022985896254425597
1102	8	-0.07378429676714404
1102	9	0.00987319460774927
1102	10-14	-0.0209997727766833
1102	15-19	-0.02403359216434353
1102	20-24	-0.1922089815021053
1102	25-29	-0.04165078235566
1102	30-34	-0.12193412344404919
1102	35-39	-0.07138297568924656
1102	40-44	-0.08817035254077865
1102	45-49	0.053646062128407834
1102	50-54	-0.0878807586823811
1102	55-59	-0.22028199414269523
1102	60-64	0.028339556117686016
1102	65-69	-0.03658527767790076
1102	70-74	-4.766518946297538E-5
1102	75-79	-0.18860675724356923
1102	80-84	0.050920427784404865
1102	85-89	-0.096522879682297
1102	90-94	-0.1454447184442813
1102	95-99	-3.596959926710497E-4
1102	100-104	-0.14127679362776036
1102	105-109	-0.12535571861547368
1102	110-114	-0.06389865537657613
1102	115-119	-0.03260550940132845
1102	120-124	-0.33663161446770573
1102	125-129	-0.023226930538228885
1102	130-134	-0.153311446287546
1102	135-139	-0.24408248240883523
1102	140-144	-0.17375467048974258
1102	145-149	-0.14598109557005046
1102	150-154	-0.026280586555067487
1102	155-159	-0.18623312591531516
1102	160-164	-0.5657791800911269
1102	165-169	-0.1573380975957832
1102	170-174	-0.20365467749997634
1102	175-179	-0.0796611013059092
1102	180-184	-0.0591027397464714
1102	185-189	-0.1678830805580347
1102	190-194	-0.3253794237319809
1102	195-199	-0.173943840539323
1102	200-204	-0.30325617699912755
1102	205-209	-0.14937141792022146
1102	210-214	0.13782699348566751
1102	215-219	-0.39972905313843654
1102	220-224	-0.15930608396903523
1102	225-229	-0.4551713960496926
1102	230-234	-0.1884325170300798
1102	235-239	-0.5112960781918972
1102	240-244	-0.3018784541446138
1102	245-249	0.15317763063931977
1102	250-254	-0.3573655369366975
1102	255-259	-0.7217094464529978
1102	260-264	-0.6896874319699577
1102	265-269	-0.7911967258809582
1102	270-274	-0.5692724471214632
1102	275-279	-0.7653180844790981
1102	280-284	-0.4967963183552371
1102	285-289	-0.5036517593558258
1102	290-294	-0.5826764309682986
1102	295-299	-0.5108260546054595
1102	300-301	-2.3328712763482997
1103	1	-0.17571998975430603
1103	2	-0.06198040622458478
1103	3	-0.03684459522613537
1103	4	-0.25515696865787874
1103	5	-0.054606268254410395
1103	6	-0.13543768587299354
1103	7	-0.15240730570864258
1103	8	0.10355486732451169
1103	9	-0.18023233641537217
1103	10-14	-0.07821961303671543
1103	15-19	-0.03953713659536362
1103	20-24	-0.052989290931463984
1103	25-29	-0.1043492544546254
1103	30-34	-0.17388489701745158
1103	35-39	-0.07021539841208835
1103	40-44	-0.10981543898640211
1103	45-49	-0.18931693251484916
1103	50-54	-0.07793282920746947
1103	55-59	-0.20738304517609407
1103	60-64	-0.19351723390016673
1103	65-69	-0.06687385744630348
1103	70-74	-0.10691306234750897
1103	75-79	-0.1691257517585143
1103	80-84	-0.1351990885050114
1103	85-89	-0.238732081303219
1103	90-94	-0.012242541532799578
1103	95-99	-0.539536618164874
1103	100-104	-0.4936499051356691
1103	105-109	-0.11130629973109052
1103	110-114	-0.038079373244144676
1103	115-119	-0.07031868313725909
1103	120-124	-0.2429645450652913
1103	125-129	-0.08910309464057065
1103	130-134	-0.17091491908150402
1103	135-139	-0.4917371703599116
1103	140-144	-0.3275370007259326
1103	145-149	-0.1954042567962162
1103	150-154	-0.14866235866364264
1103	155-159	-0.0632055509904248
1103	160-164	-0.24138087799654073
1103	165-169	-0.18715622498146445
1103	170-174	-0.3459579695974426
1103	175-179	-0.14768851584931042
1103	180-184	-0.1992034593247638
1103	185-189	-0.21406012034333344
1103	190-194	-0.4813429422881157
1103	195-199	-0.26868867358218296
1103	200-204	-0.030120077263227074
1103	205-209	-0.1957195312992397
1103	210-214	-0.0215622737847454
1103	215-219	-0.5246298839145638
1103	220-224	-0.2199046277050698
1103	225-229	-0.6565763379381266
1103	230-234	-0.35318921830349836
1103	235-239	-0.3590363029398347
1103	240-244	-0.2276966670670788
1103	245-249	0.5082494280881846
1103	250-254	-0.21918084160807894
1103	255-259	0.028935821758317104
1103	260-264	-0.38950459706667573
1103	265-269	-0.6341639973640554
1103	270-274	-0.15563788170185688
1103	275-279	-0.05567487984299646
1103	280-284	-0.21391330061704394
1103	285-289	-0.3518367754474383
1103	290-294	-0.15624983757815158
1103	295-299	-0.4103074877744959
1103	300-301	-1.069199872915739
1104	1	-0.012863511352584567
1104	2	-0.12411162380823981
1104	3	0.24895467897221124
1104	4	-0.6694804641878704
1104	5	-0.7040202726568126
1104	6	-0.7416064590951308
1104	7	-0.20543084829134983
1104	8	-0.6750539948110372
1104	9	-0.3426345127386625
1104	10-14	-0.2554277104304319
1104	15-19	-0.23958148513159472
1104	20-24	-0.41534928206176147
1104	25-29	-0.39341085024475575
1104	30-34	-0.4588058538641633
1104	35-39	-0.21861209066029375
1104	40-44	-0.24189619658920947
1104	45-49	-0.42155615500929855
1104	50-54	-0.2956214051326995
1104	55-59	-0.37459649684429763
1104	60-64	-0.3108223572377682
1104	65-69	-0.27067376983090696
1104	70-74	-0.27968630611842826
1104	75-79	-0.24611913733658497
1104	80-84	-0.5740116294057245
1104	85-89	-0.39866804634359454
1104	90-94	-0.3189873048900935
1104	95-99	-0.9684453737557632
1104	100-104	-0.7468432701619818
1104	105-109	-0.594138548688619
1104	110-114	-0.37559985740035273
1104	115-119	-0.43079656565689817
1104	120-124	-0.3594346179158734
1104	125-129	-0.5133303754803862
1104	130-134	-0.5165868805307241
1104	135-139	-0.8079487236944374
1104	140-144	-0.905534718398826
1104	145-149	-0.7639351844918778
1104	150-154	-0.4897869726420936
1104	155-159	-0.4141084763713039
1104	160-164	-0.7057338490780509
1104	165-169	-0.44596130234802445
1104	170-174	-0.557550244298767
1104	175-179	-0.30545681097258637
1104	180-184	-0.6361944652252873
1104	185-189	-0.40557656986442936
1104	190-194	-0.8239818965279966
1104	195-199	-0.32450725561886884
1104	200-204	-0.41467164325330685
1104	205-209	-0.5881283599029601
1104	210-214	-0.08492659700986138
1104	215-219	-0.6132187892088083
1104	220-224	-0.7586766059202077
1104	225-229	-0.9950530198022847
1104	230-234	-0.7701346858290208
1104	235-239	-0.8922441623657846
1104	240-244	-0.7103774819984707
1104	245-249	-0.08211395949097522
1104	250-254	-0.7283150175636024
1104	255-259	-0.7676763827425148
1104	260-264	-0.815488512609754
1104	265-269	-0.8825677204777591
1104	270-274	-0.833932518233155
1104	275-279	-0.8936155934217638
1104	280-284	-0.887773083525552
1104	285-289	-1.038879972003393
1104	290-294	-1.463679299067941
1104	295-299	-1.2654980532931361
1104	300-301	-2.187795756882956
1201	1	0.22489872640965558
1201	2	0.08421338451676519
1201	3	0.08778251780005064
1201	4	0.13151853681112868
1201	5	-0.02196899060552937
1201	6	-0.007473924962596357
1201	7	0.34495210209160376
1201	8	0.23896531920828323
1201	9	0.2931297230255723
1201	10-14	0.02625061124789596
1201	15-19	0.05664229109218155
1201	20-24	0.1094192827068099
1201	25-29	0.09745828062437312
1201	30-34	-0.010720605778914205
1201	35-39	0.04468461263640933
1201	40-44	0.13960230490929604
1201	45-49	0.08123438778124381
1201	50-54	0.039123849612551
1201	55-59	0.13228329003548822
1201	60-64	-0.008711135126553415
1201	65-69	0.1034454442883046
1201	70-74	-0.0280046544367778
1201	75-79	-0.015949307166764015
1201	80-84	-0.03657573196982611
1201	85-89	-0.018181865857414437
1201	90-94	0.0492711033683193
1201	95-99	0.05948669417630015
1201	100-104	0.09251736919866005
1201	105-109	0.06192538737531095
1201	110-114	0.007683525883024345
1201	115-119	0.04075854589819983
1201	120-124	0.04272322424196773
1201	125-129	0.02193435978435332
1201	130-134	0.046197002253158814
1201	135-139	0.2099267341810247
1201	140-144	0.066306773442669
1201	145-149	-0.13885359941028952
1201	150-154	0.12542448256936467
1201	155-159	-0.0021471044099357073
1201	160-164	-0.17671620006040456
1201	165-169	0.05986620347947991
1201	170-174	0.20248971574119423
1201	175-179	0.08599174047596136
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2302	4	-0.011875158695239918
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2302	6	0.37602708107361593
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2404	15-19	0.11485367320599238
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2404	40-44	0.14736072535064437
2404	45-49	0.14892312181144263
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2404	65-69	0.05601222709898934
2404	70-74	0.21343553139365667
2404	75-79	0.1449798449819042
2404	80-84	0.29252995908708357
2404	85-89	0.24899583449102636
2404	90-94	0.3230457839722618
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2404	190-194	0.4137371324105459
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2404	205-209	0.5223156676142366
2404	210-214	0.8734996921481297
2404	215-219	0.6084220090173602
2404	220-224	0.47058713846548983
2404	225-229	0.5632572877763167
2404	230-234	0.40649161469972483
2404	235-239	0.5935617390498678
2404	240-244	0.5092893552293418
2404	245-249	1.3850985028434337
2404	250-254	0.5666852667049724
2404	255-259	0.48859515060218683
2404	260-264	0.609411140582587
2404	265-269	0.7050199763782317
2404	270-274	0.7629518981634575
2404	275-279	0.859193548655675
2404	280-284	0.8109613527698514
2404	285-289	0.6345386419030277
2404	290-294	0.7849306275907679
2404	295-299	0.5680224130382285
2404	300-301	2.2407139736236488
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	63.0
21	107.0
22	158.0
23	174.0
24	188.0
25	221.0
26	253.0
27	298.0
28	388.0
29	434.0
30	483.0
31	638.0
32	722.0
33	949.0
34	1344.0
35	1908.0
36	3020.0
37	5507.0
38	16300.0
39	97286.0
40	695.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	99.76742590036677	0.20359765443301484	0.018300912758023806	0.010675532442180552
2	0.35458018468671126	99.31142815747936	0.03507674945287897	0.2989149083810555
3	0.12505623717982936	0.0815915693795228	0.18758435576974403	99.6057678376709
4	0.12124354702190772	0.1387819217483472	99.4799490624595	0.2600254687702549
5	0.18605927970657535	98.81959112710746	0.09226710182170336	0.9020824913642568
6	0.11895593292715476	0.40033246658177074	0.1326816174956726	99.3480299829954
7	0.2691759251492668	31.562211665306805	37.35445055322134	30.814161856322585
8	34.56203628155954	33.32291198023501	0.10751786245338986	32.00753387575205
9	99.73006153681915	0.17385867120122614	0.04422720583189086	0.05185258614773412
10-14	39.99588229462944	19.86182810867692	39.85069505341579	0.291594543277846
15-19	0.1427471195125857	39.750040033246655	39.79502977711014	20.312183070130622
20-24	19.72365621735384	0.39560473078594793	39.65258767281018	40.22815137905003
25-29	20.053377662210902	0.09745236043647677	54.639967668387456	25.20920230896516
30-34	0.1854492492813079	24.24886191198786	0.5296589167384723	75.03602992199237
35-39	19.95702329099716	0.5058685016409843	44.6405695252736	34.896538682088256
40-44	26.122769559249658	18.617355497941134	21.86411468659448	33.395760256214736
45-49	13.161811804178742	19.970718316303188	20.23669360988257	46.630776269635504
50-54	39.47234774598633	0.6969682643050068	20.545160340828154	39.285523648880506
55-59	25.1723743508796	21.82508635874911	39.869604007961016	13.132935282410266
60-64	20.500385087578827	11.355127002646046	27.60216259083873	40.54232531893639
65-69	51.217258023928814	27.206094296891088	1.3044174501864434	20.272230228993664
70-74	52.88952943060416	7.112300688582344	1.3721318600873882	38.6260380207261
75-79	0.4820838957137083	0.17858913061713144	40.162270567870735	59.177056405798425
80-84	20.133598700615376	20.411166777236367	0.5682520074119828	58.886982514736275
85-89	47.18483530863332	12.706636306374595	27.235347676690342	12.873180708301751
90-94	0.14351644262240076	0.13634824623212147	65.24324588133737	34.476889429808104
95-99	13.334807640980669	39.53986349944714	7.634117512487132	39.49121134708506
100-104	0.14229610706523813	6.673580661150723	59.25115339154306	33.93296984024097
105-109	20.15236206962291	39.483890647043125	0.6006024326076181	39.76314485072635
110-114	0.15617493422808557	19.939451710069775	54.66488732985092	25.23948602585122
115-119	20.107614783001257	0.4863712343976866	19.758354787149674	59.64765919545138
120-124	0.23106311912333277	44.058169949593164	2.4963967879938687	53.214370143289635
125-129	44.71307756247474	37.51839735230644	17.50360321200613	0.26492187321269245
130-134	0.4763110373605993	19.271303594524024	52.21332877815467	28.03905658996071
135-139	0.5084952566879175	1.623859927401397	14.495165177073483	83.3724796388372
140-144	0.19857853155598937	12.973034804624348	44.14422109019919	42.684165573620476
145-149	32.800024403077835	32.859506905308436	7.160778153144564	27.179690538469163
150-154	0.5542574414475973	38.33283510154777	20.98964084605858	40.123266610946054
155-159	32.88101211765334	8.221854481396466	39.432780959498515	19.46435244145168
160-164	20.64652904347561	26.498082070601154	20.43986547803342	32.415523407889815
165-169	17.65120375807399	28.88668583325072	33.655047242833504	19.807063165841793
170-174	22.284128085654803	36.394140210934104	20.275602260335084	21.04612944307601
175-179	13.374106809221312	7.540856090474411	44.86917662490181	34.21586047540246
180-184	40.73102470049035	14.24651684193669	21.22320427663939	23.79925418093357
185-189	38.9653352693197	20.934581149756273	18.99468010468968	21.105403476234343
190-194	51.13398917105163	12.45039274002898	3.141920231831007	33.273697857088386
195-199	18.01860748875162	14.201021886677342	60.54770075497598	7.232669869595059
200-204	1.1190421718904904	39.228856859604974	18.903225806451612	40.748875162052926
205-209	19.208266605658505	26.92778159078777	15.904674750247846	37.959277053305875
210-214	1.8265779527220587	34.408995029030585	48.99797660973131	14.766450408516047
215-219	6.920308091207199	51.06550751162968	0.6919850530008389	41.32219934416228
220-224	35.843819110806066	2.956760466712423	20.259894760924276	40.939525661557234
225-229	28.496606421108822	7.126058110272249	18.884618317699992	45.492717150918935
230-234	17.432929154274383	1.371768474033402	59.629070388164415	21.566231983527796
235-239	19.56104628994128	19.336993822923816	0.22130709982460156	60.88065278731031
240-244	15.073026990092245	44.783786421982526	20.890782859095125	19.252403728830103
245-249	1.608599100528113	24.982271819657754	34.88781483071143	38.52131424910271
250-254	25.119042171890488	41.82475406085564	26.90658125524289	6.149622512010981
255-259	20.09196980096088	19.081979714786854	20.866468390147183	39.95958209410509
260-264	19.998322275604362	18.847098299397544	19.128040875467097	42.026538549531
265-269	1.4172195531152292	7.4205750019065055	20.350644398688324	70.81156104628994
270-274	18.709858476105353	20.912866488014167	59.56159469472233	0.8156803411581502
275-279	21.45762696353645	18.816089902858423	0.3024521041240557	59.42383102948108
280-284	58.3417729241657	0.20926117991580748	20.044994204136415	21.403971691782075
285-289	40.03813068147154	1.4619303276188151	19.912146909889575	38.58779208102007
290-294	34.16326032578854	38.660697943993654	7.075834299310597	20.100207430907204
295-299	38.42575428157303	20.045619254682077	39.86451539077698	1.6641110729679216
300-301	7.311195551954403	2.7353245229247576	0.30035483780838756	89.65312508731246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.3333333333333333
45	5.999999999999998
46	32.41666666666666
47	940.75
48	963.25
49	82.91666666666667
50	75.66666666666667
51	684.8333333333328
52	7675.416666666663
53	3630.583333333334
54	629.6666666666667
55	2337.166666666666
56	18871.833333333354
57	6585.999999999991
58	151.58333333333334
59	20.083333333333332
60	7.0
61	1.25
62	0.75
63	1.75
64	1.0
65	0.5
66	0.75
67	0.75
68	0.25
69	0.75
70	0.75
71	1.25
72	1.5
73	1.25
74	1.75
75	0.75
76	1.75
77	1.8333333333333333
78	0.75
79	1.25
80	0.75
81	0.5833333333333333
82	0.6666666666666666
83	1.75
84	1.5833333333333333
85	0.75
86	0.5833333333333333
87	1.0833333333333333
88	0.75
89	1.0
90	2.0
91	1.25
92	1.25
93	1.0833333333333333
94	1.6666666666666665
95	1.3333333333333333
96	0.5
97	0.75
98	1.75
99	5.75
100	12.666666666666671
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	1.525145842071148E-4
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	4.5755770946610645E-4
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.6509570655075116
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	3.0504079920689394E-4
245-249	1.9226721574010526
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	1.5252039960344697E-4
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-39	1.0
40-49	0.0
50-59	1.0
60-69	0.0
70-79	0.0
80-89	4.0
90-99	0.0
100-109	0.0
110-119	2.0
120-129	0.0
130-139	1.0
140-149	1.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	1.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	2.0
280-289	0.0
290-299	321.0
300-302	130807.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.585141183916548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.597704972559455	2.8701931508834004
2	11.425245301846001	1.0477272553968628
3	5.438217196075171	0.7480498089842231
4	3.5589555962082153	0.6527325550361824
5	2.5278563113254613	0.5795289040040872
6	2.3781806086811907	0.6542576310993511
7	1.5133876600698486	0.48573672611921515
8	1.5965408282055547	0.5856292082567618
9	1.197405621154166	0.4941246444666428
>10	6.55246964909363	5.585591081355182
>50	0.3326126725428239	1.0157006580703212
>100	0.36587393979710625	3.4825111902456136
>500	0.2161982371528355	5.505524588038829
>1k	0.14967570264427074	20.48024645229181
>5k	0.14967570264427074	55.812446145751515
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	9336	7.11905506287126	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	9115	6.950534157891125	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	8972	6.841491219374567	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	8121	6.192571354496305	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	7805	5.951609336515658	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	7748	5.9081446687153525	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	7743	5.904331978557431	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	7419	5.657269656324109	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	6934	5.287438711005711	No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	3489	2.6604951921977107	No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	3400	2.592629307386706	No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	3290	2.50875012391243	No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	3016	2.299814703258325	No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	2880	2.196109530962857	No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	2803	2.137394102530864	No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	2752	2.0985046629200634	No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	2674	2.039026696456486	No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	2554	1.9475221326663665	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	628	0.4788738838349562	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	620	0.47277357958228167	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	600	0.45752281895059516	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	584	0.44532221044524595	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	578	0.44074698225574005	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	557	0.42473368359246916	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	535	0.40795784689761405	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	529	0.40338261870810804	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	528	0.4026200806765238	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	525	0.40033246658177074	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	524	0.39956992855018647	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	509	0.3881318580764216	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	503	0.38355662988691563	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	495	0.37745632563424103	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	475	0.3622055650025545	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	472	0.3599179509078015	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	454	0.3461922663392837	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	424	0.3233161253917539	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	181	0.13801938371676287	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	179	0.13649430765359422	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	171	0.1303940034009196	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	150	0.11438070473764879	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	149	0.11361816670606445	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	141	0.10751786245338986	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
