##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	JAFS1_cleaned_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	131141
Sequences flagged as poor quality	0
Sequence length	38-301
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.56718341327274	40.0	40.0	40.0	40.0	40.0
2	39.2551375999878	40.0	40.0	40.0	40.0	40.0
3	39.14858053545421	40.0	40.0	40.0	40.0	40.0
4	38.78320281224026	40.0	40.0	40.0	40.0	40.0
5	39.30935405403344	40.0	40.0	40.0	40.0	40.0
6	39.26137516108616	40.0	40.0	40.0	40.0	40.0
7	39.25823350439603	40.0	40.0	40.0	40.0	40.0
8	39.41671940888052	40.0	40.0	40.0	40.0	40.0
9	39.06830053148901	40.0	40.0	40.0	40.0	40.0
10-14	39.55384204787214	40.0	40.0	40.0	40.0	40.0
15-19	39.4769965152012	40.0	40.0	40.0	40.0	40.0
20-24	39.45227198206511	40.0	40.0	40.0	40.0	40.0
25-29	39.6175109233573	40.0	40.0	40.0	40.0	40.0
30-34	39.441053522544436	40.0	40.0	40.0	40.0	40.0
35-39	39.609781183135425	40.0	40.0	40.0	40.0	40.0
40-44	39.530852524020126	40.0	40.0	40.0	40.0	40.0
45-49	39.456623455848714	40.0	40.0	40.0	40.0	40.0
50-54	39.564793093592954	40.0	40.0	40.0	40.0	40.0
55-59	39.47316969017608	40.0	40.0	40.0	40.0	40.0
60-64	39.44241148704809	40.0	40.0	40.0	40.0	40.0
65-69	39.506520562151614	40.0	40.0	40.0	40.0	40.0
70-74	39.361160295564254	40.0	40.0	40.0	40.0	40.0
75-79	39.31592584967096	40.0	40.0	40.0	40.0	40.0
80-84	39.47911147713494	40.0	40.0	40.0	40.0	40.0
85-89	39.59281497012479	40.0	40.0	40.0	40.0	40.0
90-94	39.41270293971861	40.0	40.0	40.0	40.0	40.0
95-99	39.29164296335837	40.0	40.0	40.0	40.0	40.0
100-104	39.27586075418462	40.0	40.0	40.0	40.0	40.0
105-109	39.34771342509627	40.0	40.0	40.0	40.0	40.0
110-114	39.30960918137797	40.0	40.0	40.0	40.0	40.0
115-119	39.25807967350033	40.0	40.0	40.0	40.0	40.0
120-124	39.17795215544523	40.0	40.0	40.0	40.0	40.0
125-129	39.27719795932374	40.0	40.0	40.0	40.0	40.0
130-134	39.32372647487226	40.0	40.0	40.0	40.0	40.0
135-139	39.31046579019614	40.0	40.0	40.0	40.0	40.0
140-144	39.41032242320715	40.0	40.0	40.0	40.0	40.0
145-149	39.052849440635704	40.0	40.0	40.0	36.8	40.0
150-154	39.28940067566022	40.0	40.0	40.0	40.0	40.0
155-159	39.141896271667264	40.0	40.0	40.0	40.0	40.0
160-164	39.376117012758236	40.0	40.0	40.0	40.0	40.0
165-169	39.299125302178744	40.0	40.0	40.0	40.0	40.0
170-174	39.06641755191374	40.0	40.0	40.0	40.0	40.0
175-179	39.31889789599713	40.0	40.0	40.0	40.0	40.0
180-184	39.321167382236084	40.0	40.0	40.0	40.0	40.0
185-189	39.244526614174376	40.0	40.0	40.0	40.0	40.0
190-194	39.347956989247315	40.0	40.0	40.0	40.0	40.0
195-199	39.269006329596586	40.0	40.0	40.0	40.0	40.0
200-204	39.49136887058644	40.0	40.0	40.0	40.0	40.0
205-209	39.14769770456799	40.0	40.0	40.0	40.0	40.0
210-214	39.31440860215054	40.0	40.0	40.0	40.0	40.0
215-219	39.30023640661939	40.0	40.0	40.0	40.0	40.0
220-224	39.2447677876916	40.0	40.0	40.0	40.0	40.0
225-229	39.34607183710821	40.0	40.0	40.0	40.0	40.0
230-234	39.14377487989018	40.0	40.0	40.0	40.0	40.0
235-239	39.33560893769541	40.0	40.0	40.0	40.0	40.0
240-244	39.25067337756425	40.0	40.0	40.0	40.0	40.0
245-249	39.11497292762907	40.0	40.0	40.0	40.0	40.0
250-254	39.26406466864943	40.0	40.0	40.0	40.0	40.0
255-259	39.12690612369404	40.0	40.0	40.0	40.0	40.0
260-264	39.14447647372836	40.0	40.0	40.0	40.0	40.0
265-269	39.1721558758484	40.0	40.0	40.0	40.0	40.0
270-274	37.940114390299705	40.0	36.8	40.0	33.6	40.0
275-279	39.105590393980385	40.0	40.0	40.0	40.0	40.0
280-284	38.04492709413702	40.0	36.8	40.0	36.8	40.0
285-289	39.04344457324141	40.0	40.0	40.0	40.0	40.0
290-294	39.13000734228409	40.0	40.0	40.0	40.0	40.0
295-299	39.00949996938418	40.0	40.0	40.0	40.0	40.0
300-301	38.29349905590924	40.0	40.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.11997166482291277
1101	2	0.17473675187158477
1101	3	-0.26648147937667943
1101	4	0.26039667163330193
1101	5	0.005839255919745767
1101	6	-0.043811063852317034
1101	7	0.04587910426931785
1101	8	0.09436472065112156
1101	9	-0.07744436251461906
1101	10-14	-0.09384605799411361
1101	15-19	-5.702329263712613E-4
1101	20-24	-0.044759188778229486
1101	25-29	-0.08497383296136007
1101	30-34	-0.021363300335814017
1101	35-39	-0.05305803734465542
1101	40-44	-0.07502346060965692
1101	45-49	-0.11688504738339844
1101	50-54	-0.01472726658520429
1101	55-59	-0.22787531738283207
1101	60-64	-0.09286973454769054
1101	65-69	-0.12241367868239195
1101	70-74	-0.13338403670577748
1101	75-79	-0.14160460813479858
1101	80-84	-0.11534952901467932
1101	85-89	-0.0776648869307337
1101	90-94	-0.2903277781728022
1101	95-99	-0.03239242996492209
1101	100-104	-0.12257252154869036
1101	105-109	-0.07310630626326287
1101	110-114	-0.23374440719469192
1101	115-119	-0.08436200843974007
1101	120-124	-0.2130638435237202
1101	125-129	-0.11651461039276967
1101	130-134	-0.14314653007200917
1101	135-139	-0.18099677899173372
1101	140-144	-0.047368424060529435
1101	145-149	0.030405902410180374
1101	150-154	0.005566074953335942
1101	155-159	-0.02145519493105752
1101	160-164	-0.10770395328272997
1101	165-169	0.007466168687194852
1101	170-174	0.05204975137996115
1101	175-179	-0.03615927812254682
1101	180-184	-0.05085388098503074
1101	185-189	-0.028359899676317468
1101	190-194	-0.047948884957492055
1101	195-199	0.029168685841035824
1101	200-204	-0.04975290407383426
1101	205-209	-0.09683152283434993
1101	210-214	-0.14943038335143655
1101	215-219	-0.15756187443478353
1101	220-224	-0.012168026648645025
1101	225-229	-0.0698791086483368
1101	230-234	0.016972710000736413
1101	235-239	-0.20490778884422411
1101	240-244	-0.14242271880061708
1101	245-249	-0.29326300937939465
1101	250-254	-0.04941225622376777
1101	255-259	-0.09535419678817192
1101	260-264	-0.07558179825170441
1101	265-269	-0.03661297523628804
1101	270-274	0.1276022128979193
1101	275-279	-0.14392279279680764
1101	280-284	0.14614205841516537
1101	285-289	-0.018268353920944946
1101	290-294	-0.2778845956365572
1101	295-299	-0.2096709305959905
1101	300-301	-0.08661097847549826
1102	1	0.2728764015457301
1102	2	0.3225043729337571
1102	3	0.23151655643203384
1102	4	0.017743502486212037
1102	5	0.09951607090803805
1102	6	-0.12268089840697627
1102	7	0.2696458187816688
1102	8	0.10252366905332622
1102	9	0.34470567148100173
1102	10-14	0.00735512052065701
1102	15-19	-0.01042140766384847
1102	20-24	-0.0036320451656308705
1102	25-29	-0.009035176166015901
1102	30-34	-0.05254861422868373
1102	35-39	0.08162504794656655
1102	40-44	0.10362729107123414
1102	45-49	-0.1354995370714036
1102	50-54	0.06840335213505
1102	55-59	-0.025352087043621907
1102	60-64	0.0635101098279307
1102	65-69	0.06868702389372316
1102	70-74	0.034267243795852664
1102	75-79	0.09131825958447592
1102	80-84	0.10173893513882604
1102	85-89	-0.017772161993057978
1102	90-94	0.10785912218226912
1102	95-99	0.11658392419386132
1102	100-104	0.017187242748349263
1102	105-109	0.19713619581424524
1102	110-114	0.10977753886558617
1102	115-119	0.09537660302811446
1102	120-124	0.16895625166400663
1102	125-129	0.06878417331954978
1102	130-134	0.19671899837099005
1102	135-139	-0.0026482105850007542
1102	140-144	0.005574085571566911
1102	145-149	0.13405476544561168
1102	150-154	0.06131888903507132
1102	155-159	0.01513919956919807
1102	160-164	0.07928705676486913
1102	165-169	0.09062700573485927
1102	170-174	0.02766356115558466
1102	175-179	0.08622494791054436
1102	180-184	-0.057350821379387185
1102	185-189	-0.09118380237330825
1102	190-194	-0.04009211982943839
1102	195-199	0.06491696717365869
1102	200-204	0.06256861892987331
1102	205-209	0.029511304090178214
1102	210-214	0.09651658348394676
1102	215-219	0.03891769042130022
1102	220-224	-0.007000692660582786
1102	225-229	0.13016621884278834
1102	230-234	-0.0021857095807504834
1102	235-239	-0.05043169909578182
1102	240-244	-0.07769506147644734
1102	245-249	-0.09140458224334225
1102	250-254	0.002502830276185364
1102	255-259	-0.03875526953879671
1102	260-264	-0.06379665055963102
1102	265-269	-0.07982518344056899
1102	270-274	0.06665184650070444
1102	275-279	-0.09004351501482688
1102	280-284	-0.24077340581388995
1102	285-289	0.01989739360492848
1102	290-294	0.017257808231384786
1102	295-299	-0.19467072311098832
1102	300-301	-0.26997401617506256
1103	1	0.1973474099176471
1103	2	0.21269650996370615
1103	3	0.49603910072406165
1103	4	0.009403664792287714
1103	5	0.012011927719434823
1103	6	0.2701040727778192
1103	7	0.11762554656080937
1103	8	0.2382705656714137
1103	9	0.024926356416898443
1103	10-14	0.0969677457518685
1103	15-19	0.20854423862010663
1103	20-24	0.07224337048974405
1103	25-29	0.10509229504550888
1103	30-34	0.15664872985377798
1103	35-39	0.10717061232904967
1103	40-44	0.039705642777164485
1103	45-49	0.21791284651836662
1103	50-54	0.09727629791540693
1103	55-59	0.0745377843304098
1103	60-64	0.03953842250768247
1103	65-69	0.16573707361197876
1103	70-74	0.13013688571743387
1103	75-79	0.07037885171294533
1103	80-84	0.009564482841625477
1103	85-89	0.11271422161809141
1103	90-94	0.015158618584372618
1103	95-99	0.11405203500679306
1103	100-104	0.0713363120010797
1103	105-109	0.1325088689460685
1103	110-114	0.1259354397925989
1103	115-119	0.107360308268305
1103	120-124	0.058459817479224796
1103	125-129	0.013972193891149232
1103	130-134	-0.005391622091543979
1103	135-139	0.03697243372092629
1103	140-144	0.01809667263198378
1103	145-149	0.029853839509783597
1103	150-154	-0.011939100636354283
1103	155-159	0.04812646527857822
1103	160-164	-0.12128817819402116
1103	165-169	-0.03616418825189527
1103	170-174	-0.026947406800196916
1103	175-179	-0.06650100288442218
1103	180-184	-0.06440333028055534
1103	185-189	-0.03401528419139055
1103	190-194	-0.048731336312378914
1103	195-199	0.015532297506076986
1103	200-204	-0.04080687691624263
1103	205-209	-0.15273767214128497
1103	210-214	0.10531404304107639
1103	215-219	-0.023293221935560382
1103	220-224	-0.0309381652620786
1103	225-229	-0.003562009369396435
1103	230-234	-0.06591062324203278
1103	235-239	-0.03329012500488204
1103	240-244	-0.10226122953486794
1103	245-249	0.0668384919491416
1103	250-254	0.02871985851403025
1103	255-259	-0.00630688564965709
1103	260-264	-0.08524072553263551
1103	265-269	-0.017460304849350905
1103	270-274	-0.3667331653140664
1103	275-279	-0.06359981426530936
1103	280-284	0.011012709425557432
1103	285-289	0.15430563935214536
1103	290-294	0.04282047997323701
1103	295-299	-0.03052988677495705
1103	300-301	-0.27928940280769154
1104	1	-0.0649363233637672
1104	2	-0.22344430204717725
1104	3	0.037302168669263835
1104	4	0.13431510292878102
1104	5	0.22339219478416084
1104	6	-0.3103641828644541
1104	7	-0.05327856865884684
1104	8	0.050102971471339686
1104	9	-0.473572824216852
1104	10-14	-0.029696000401436606
1104	15-19	0.011234355927392414
1104	20-24	-0.06617165609234377
1104	25-29	-0.02119935607213108
1104	30-34	0.0503420435006845
1104	35-39	-0.12361917020087532
1104	40-44	-0.06307912079646627
1104	45-49	-0.18087351792924977
1104	50-54	-0.13189097719153153
1104	55-59	-0.10673307165041734
1104	60-64	-0.04686983281008139
1104	65-69	-0.08582513061843144
1104	70-74	-0.20613751273793923
1104	75-79	-0.15260126175440547
1104	80-84	-0.03022372585609645
1104	85-89	-0.10324367972264525
1104	90-94	-0.0827932362120336
1104	95-99	-0.2301575064185286
1104	100-104	-0.08111015877486238
1104	105-109	-0.024831084217552757
1104	110-114	-0.10061851669175326
1104	115-119	-0.06730802417263959
1104	120-124	-0.18389519796163256
1104	125-129	-0.1069110991499187
1104	130-134	-0.1671880623102595
1104	135-139	-0.13583975022815054
1104	140-144	-0.03126649320449815
1104	145-149	-0.21695766298617514
1104	150-154	-0.12537183959113207
1104	155-159	-0.10864669195862575
1104	160-164	-0.15275616882674825
1104	165-169	-0.08811038590575748
1104	170-174	-0.38763297026997634
1104	175-179	-0.20990902564279423
1104	180-184	-0.2152359470064482
1104	185-189	-0.21488375833132523
1104	190-194	-0.10615079233971869
1104	195-199	-0.1427162598144065
1104	200-204	-0.10493269695854224
1104	205-209	-0.1183881083253695
1104	210-214	-0.022396761235853546
1104	215-219	-0.11435699188564286
1104	220-224	-0.18756421515958976
1104	225-229	0.03243169530180978
1104	230-234	-0.2195339527354392
1104	235-239	-0.17650777355895997
1104	240-244	-0.3133905487848381
1104	245-249	-0.2596922085954816
1104	250-254	-0.15002421257342746
1104	255-259	-0.02731294841905907
1104	260-264	-0.11959461603501609
1104	265-269	-0.21160737973889354
1104	270-274	-0.3277107801199861
1104	275-279	-0.36837593528272805
1104	280-284	-0.578147859317383
1104	285-289	-0.14976090508562834
1104	290-294	-0.2016329784013351
1104	295-299	-0.37099142338726665
1104	300-301	-0.7742859547954808
1201	1	0.15244629401885135
1201	2	0.24109116248828855
1201	3	-0.20012706875997566
1201	4	-0.05076648215280244
1201	5	0.36618273757470376
1201	6	-0.12421699671726572
1201	7	0.005129689749409749
1201	8	0.13355285492121993
1201	9	0.2863339356899104
1201	10-14	0.0053889146834862345
1201	15-19	0.17151407620711723
1201	20-24	-0.03963818955887888
1201	25-29	0.11846098358820711
1201	30-34	-0.09390038074174356
1201	35-39	0.0953884888067904
1201	40-44	0.1117993340819865
1201	45-49	-0.013840550896283332
1201	50-54	0.09937109407052702
1201	55-59	0.06761258269522585
1201	60-64	0.0445853786451309
1201	65-69	0.07440130960801383
1201	70-74	0.06099535439492598
1201	75-79	0.0938989047457639
1201	80-84	0.13270667707431016
1201	85-89	0.1099697734908105
1201	90-94	-0.07512090853970221
1201	95-99	0.11271295645192936
1201	100-104	0.13872334105864326
1201	105-109	0.09335739397092624
1201	110-114	0.169378153304919
1201	115-119	0.1146699978053789
1201	120-124	0.11980110573467329
1201	125-129	0.1529623607235422
1201	130-134	0.12089718577499298
1201	135-139	0.1813763669879549
1201	140-144	0.0960195540815576
1201	145-149	0.10234969632118407
1201	150-154	0.22795483373553793
1201	155-159	-0.00974559305752365
1201	160-164	0.13335771728714008
1201	165-169	0.07200949421412162
1201	170-174	-0.017989280626281356
1201	175-179	0.0030913073575433714
1201	180-184	0.003478671708180059
1201	185-189	0.02893908549151547
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2404	200-204	-0.01655018516139961
2404	205-209	-0.10218252529051597
2404	210-214	-0.10266965906971848
2404	215-219	-0.03548042130174878
2404	220-224	0.05900807433953048
2404	225-229	0.01822736304868755
2404	230-234	0.00629355309206403
2404	235-239	-0.048714266253256255
2404	240-244	-0.018924689440922293
2404	245-249	0.07840546608755972
2404	250-254	-0.08335982005537801
2404	255-259	-0.16001861673448303
2404	260-264	0.019278543325953024
2404	265-269	0.023184819931366007
2404	270-274	-0.14581527724887167
2404	275-279	-0.12717063651646043
2404	280-284	0.05424738771201021
2404	285-289	-0.016591310452611197
2404	290-294	-0.007766357217640518
2404	295-299	0.19155776985977013
2404	300-301	0.15599373893852686
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	18.0
21	27.0
22	55.0
23	94.0
24	203.0
25	270.0
26	272.0
27	265.0
28	317.0
29	355.0
30	397.0
31	431.0
32	602.0
33	699.0
34	916.0
35	1214.0
36	1680.0
37	2636.0
38	5774.0
39	105428.0
40	9488.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0602405044951617	0.00838791834742758	0.11819339489557042	99.81317818226184
2	0.008387982308982765	0.021351227695592497	0.15708403233185908	99.81317675766357
3	0.011438070473764878	0.12429369914824502	99.62178113633418	0.24248709404381544
4	0.0815915693795228	99.34192967874273	0.10523024835863688	0.471248503519113
5	0.18148405151706942	0.13191907946408826	0.10980547654814285	99.5767913924707
6	99.75751290595618	0.08082903134793848	0.024401217010698407	0.13725684568517857
7	99.87189361069383	0.06862842284258928	0.043464667800306535	0.016013298663270833
8	99.72472377059806	0.12963146536933529	0.08769187363219741	0.05795289040040873
9	30.529735170541635	19.806162832371264	27.04951159439077	22.614590402696336
10-14	59.815313288750275	11.12710746448479	8.893633569974302	20.16394567679063
15-19	35.50590585705462	24.048771932500134	19.923898704447886	20.52142350599736
20-24	59.775813818714205	39.801892619394394	0.358240367238316	0.06405319465308333
25-29	14.902128243646152	15.087272477714825	59.78099907732898	10.22960020131004
30-34	1.068925812674907	59.10630542698318	0.33536422629078627	39.48940453405114
35-39	56.540756194868415	5.055329843953979	19.742749777338556	18.66116418383905
40-44	38.487418026536524	46.86502973921001	0.20680189110873876	14.440750343144732
45-49	20.203446698185147	19.90590208937014	24.87814549336587	35.012505719078845
50-54	44.712420523504	20.16998705194625	19.26453834621784	15.853054078331914
55-59	20.489404372459756	26.71852004361784	13.170605235665972	39.62147034825643
60-64	39.97788605982965	0.11529750875025736	0.4383135451696291	59.46850288625046
65-69	27.67109708019735	20.51182333249453	50.7171779562144	1.099901631093725
70-74	40.18209685905795	59.008075400910485	0.7073410655868964	0.1024866744446732
75-79	44.30276271742197	20.004880317830697	7.6637766034513	28.02858036129603
80-84	40.00366023837302	0.4654603131028908	39.33795438427928	20.19292506424481
85-89	33.5875219809633	2.060300664499963	53.805432824803525	10.546744529733209
90-94	19.726541350516644	59.21622755175964	13.542685019254966	7.514546078468753
95-99	6.771189995043277	12.91142715522172	59.93319861211729	20.384184237617724
100-104	36.77980706905098	20.858809623670265	12.144431311244137	30.21695199603462
105-109	66.84698974339422	28.134822892439086	4.750829298051626	0.26735806611507223
110-114	52.38845464597552	45.99534830518168	0.9942425744461815	0.6219544743966142
115-119	34.91776375992875	43.68068180431679	19.069900804060566	2.3316536316938956
120-124	30.6815218137311	32.844669152692305	33.51010043238544	2.9637086011911573
125-129	4.679066291475068	49.71197181487497	25.04541191004553	20.56354998360443
130-134	22.263537421606188	11.981746748334512	43.309225456941356	22.445490373117938
135-139	2.84202177958088	20.661775920446573	44.3772687063417	32.11893359363084
140-144	39.828417167434345	18.740353231857977	19.946466156239513	21.484763444468168
145-149	4.982345898376432	28.05820134064409	37.506920560355674	29.452532200623804
150-154	30.359716619258602	17.989948982315397	16.930855404137844	34.71947899428815
155-159	21.75534389274847	73.77279209340278	0.14718106321159757	4.32468295063715
160-164	22.6311093486666	17.932144191686174	4.721385484744264	54.71536097490296
165-169	38.47572275053191	22.227390929681007	21.116745849570275	18.180140470216806
170-174	2.9332499561507195	26.37926958537646	20.64286858179988	50.04461187667295
175-179	44.0107983619434	2.1300836568012143	18.7552905110157	35.103827470239686
180-184	38.67537043109562	39.062311734067464	0.6619334863609673	21.600384348475952
185-189	39.0467809142655	1.4899672387181004	6.185598457718424	53.27765338929799
190-194	21.920079310607793	36.67154731945398	37.167543658964384	4.240829710973842
195-199	31.524288873636845	40.31983527796843	9.441317776252573	18.714558072142147
200-204	76.30702356440175	0.770533058796614	20.659498207885303	2.2629451689163425
205-209	21.14192023183101	40.30183787081522	2.837336993822924	35.71890490353085
210-214	29.310150232593607	28.47876153435522	41.39708686036758	0.8140013726835964
215-219	39.6902310684054	19.897201250667276	15.774117288187295	24.63845039274003
220-224	8.324105849157325	30.91283459162663	45.951956074124915	14.811103485091131
225-229	56.01189659116906	2.2246625486158775	22.041790589491345	19.72165027072371
230-234	20.274536719286203	57.03820636010066	20.936932814763974	1.7503241058491572
235-239	44.19614123389003	20.597117364447495	3.905132311446656	31.301609090215816
240-244	37.969800960878516	23.273697857088386	8.104476473728361	30.652024708304737
245-249	54.26447037291238	4.725997102112407	20.937085335163577	20.072447189811637
250-254	18.474948524365136	11.105925417524594	62.043010752688176	8.3761153054221
255-259	17.752916952642416	39.68550293601769	19.978494623655916	22.583085487683977
260-264	38.96621673148783	31.847632120796156	26.37535270342408	2.8107984442919243
265-269	21.580721421490125	57.424693052695794	18.51018073667353	2.4844047891405476
270-274	7.580263860291314	3.3989171051628158	26.017844886753604	63.002974147792266
275-279	48.74161316940216	7.829132101256634	18.69437695324118	24.73487777610003
280-284	2.9436886096028307	37.61820511256177	6.4239521688731624	53.01415410896223
285-289	53.95582941858337	26.70276371179306	16.44103471417241	2.9003721554511626
290-294	23.736856073432108	20.11588924173233	24.749018495300675	31.398236189534888
295-299	23.59547990185906	26.507360570291617	30.82513457759115	19.072024950258168
300-301	8.784274542205484	59.55536767672886	31.63307265146129	0.027285129604365622
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.3333333333333333
46	1.833333333333333
47	3.666666666666667
48	10.5
49	168.08333333333331
50	7344.083333333321
51	5129.999999999999
52	257.00000000000017
53	365.9166666666666
54	11583.333333333341
55	18538.750000000047
56	1918.0833333333276
57	146.41666666666666
58	31.083333333333336
59	13.166666666666668
60	3.166666666666667
61	0.75
62	1.75
63	0.75
64	2.25
65	0.5
66	0.0
67	0.5
68	0.25
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.625380315843252E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	3.0505103503816186E-4
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-39	1.0
40-49	0.0
50-59	1.0
60-69	0.0
70-79	0.0
80-89	4.0
90-99	0.0
100-109	0.0
110-119	2.0
120-129	0.0
130-139	1.0
140-149	1.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	1.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	2.0
280-289	0.0
290-299	746.0
300-302	130382.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	3.6510320952257493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.22389306599833	2.0527523810250035
2	14.097744360902256	1.029426342638839
3	6.8504594820384295	0.7503374230789761
4	3.989139515455305	0.5825790561304245
5	2.8404344193817876	0.5185258614773411
6	1.7961570593149538	0.3934696242975118
7	1.7961570593149538	0.4590478950137638
8	1.461988304093567	0.4270212976872222
9	1.2113617376775272	0.39804485248701776
>10	8.500417710944026	5.802151882325131
>50	0.1670843776106934	0.405670232802861
>100	0.4177109440267335	3.449722054887488
>500	0.27151211361737676	6.663057319983833
>1k	0.2297410192147034	24.819850390038205
>5k	0.0835421888053467	26.543186341418778
>10k+	0.06265664160401002	25.705157044707605
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	13140	10.019749735018033	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	10431	7.954034207456097	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	10139	7.731373102233475	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9361	7.138118513660868	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8858	6.754561883773953	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8485	6.470135197993001	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8105	6.180370745990956	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	4586	3.496999412845716	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	3987	3.040239131926705	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3937	3.0021122303474885	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3663	2.7931768096933833	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3325	2.5354389550178813	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3220	2.4553724617015273	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3094	2.3592926697219023	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2880	2.196109530962857	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1540	1.1743085686398609	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1264	0.9638480719225871	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1053	0.8029525472582946	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	830	0.63290656621499	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	820	0.6252811858991467	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	818	0.6237561098359781	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	784	0.597829816762111	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	763	0.5818165180988402	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	749	0.5711409856566596	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	699	0.5330140840774433	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	639	0.48726180218238385	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	543	0.4140581511502886	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	532	0.405670232802861	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	530	0.4041451567396924	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	518	0.39499470036068046	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	513	0.39118201020275883	No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	456	0.3477173424024523	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	355	0.27070100121243545	No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	348	0.2653632349913452	No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	312	0.2379118658543095	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	293	0.2234236432542073	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	276	0.21046049671727377	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	272	0.2074103445909365	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	254	0.19368466002241863	No Hit
CCTACGGGAGGCAGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	225	0.1715710571064732	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	212	0.16165806269587696	No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	200	0.15250760631686505	No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	192	0.14640730206419045	No Hit
CCTACGGGGGGCAGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	182	0.1387819217483472	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	176	0.13420669355884127	No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	161	0.12276862308507636	No Hit
CCTACGGGGGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	159	0.12124354702190772	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
