##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IVMS2_cleaned_R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	109319
Sequences flagged as poor quality	0
Sequence length	15-301
%GC	52
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.041914031412656	34.0	34.0	34.0	20.0	34.0
2	32.45536457523395	34.0	34.0	34.0	34.0	34.0
3	32.63146388093561	34.0	34.0	34.0	34.0	34.0
4	33.29024231835271	34.0	34.0	34.0	34.0	34.0
5	32.57854535808048	34.0	34.0	34.0	34.0	34.0
6	32.83158462847263	34.0	34.0	34.0	34.0	34.0
7	32.412416871724034	34.0	34.0	34.0	34.0	34.0
8	32.515857261775174	34.0	34.0	34.0	34.0	34.0
9	32.957473083361535	34.0	34.0	34.0	34.0	34.0
10-14	32.94739981156066	34.0	34.0	34.0	34.0	34.0
15-19	32.979781516364554	34.0	34.0	34.0	34.0	34.0
20-24	32.808471072535916	34.0	34.0	34.0	34.0	34.0
25-29	32.944233439977495	34.0	34.0	34.0	34.0	34.0
30-34	32.65419565123749	34.0	34.0	34.0	31.2	34.0
35-39	32.5878068961847	34.0	34.0	34.0	31.2	34.0
40-44	32.80808110504322	34.0	34.0	34.0	34.0	34.0
45-49	32.70357574736863	34.0	34.0	34.0	34.0	34.0
50-54	32.69507601583131	34.0	34.0	34.0	34.0	34.0
55-59	32.62301873052114	34.0	34.0	34.0	34.0	34.0
60-64	32.570223032592416	34.0	34.0	34.0	34.0	34.0
65-69	31.967889610910106	34.0	34.0	34.0	22.8	34.0
70-74	31.736733109508158	34.0	34.0	34.0	22.8	34.0
75-79	32.53029222213125	34.0	34.0	34.0	34.0	34.0
80-84	32.353127483325665	34.0	34.0	34.0	31.2	34.0
85-89	32.31289724528545	34.0	34.0	34.0	31.2	34.0
90-94	32.31983357374925	34.0	34.0	34.0	28.4	34.0
95-99	31.733786489948123	34.0	34.0	34.0	20.0	34.0
100-104	32.10863784274467	34.0	34.0	34.0	20.0	34.0
105-109	31.761899186522744	34.0	34.0	34.0	20.0	34.0
110-114	32.214817308522655	34.0	34.0	34.0	25.6	34.0
115-119	31.965401740301523	34.0	34.0	34.0	20.0	34.0
120-124	31.82766803442068	34.0	34.0	34.0	20.0	34.0
125-129	31.63740830429058	34.0	34.0	34.0	20.0	34.0
130-134	31.57607647188707	34.0	34.0	34.0	25.6	34.0
135-139	31.496361336224016	34.0	34.0	34.0	20.0	34.0
140-144	31.674948129458436	34.0	34.0	34.0	20.0	34.0
145-149	31.1272321126064	34.0	34.0	34.0	20.0	34.0
150-154	31.28768697275523	34.0	34.0	34.0	20.0	34.0
155-159	31.252355147455887	34.0	34.0	34.0	20.0	34.0
160-164	30.98215234186112	34.0	34.0	34.0	20.0	34.0
165-169	31.206864398460375	34.0	34.0	34.0	20.0	34.0
170-174	30.570473159486323	34.0	31.2	34.0	17.8	34.0
175-179	31.034400220533342	34.0	34.0	34.0	20.0	34.0
180-184	31.156453014135487	34.0	34.0	34.0	20.0	34.0
185-189	30.487852654231084	34.0	34.0	34.0	20.0	34.0
190-194	30.317914779397448	34.0	31.2	34.0	17.8	34.0
195-199	31.08188717382584	34.0	34.0	34.0	20.0	34.0
200-204	29.7651496561839	34.0	31.2	34.0	17.8	34.0
205-209	29.688826472735855	34.0	31.2	34.0	20.0	34.0
210-214	29.354578602164064	34.0	25.6	34.0	17.8	34.0
215-219	29.402209237039937	34.0	25.6	34.0	15.6	34.0
220-224	29.630106868685413	34.0	22.8	34.0	20.0	34.0
225-229	28.81197509205473	34.0	22.8	34.0	15.6	34.0
230-234	29.611515545731702	34.0	22.8	34.0	20.0	34.0
235-239	28.81372697538084	34.0	20.0	34.0	15.6	34.0
240-244	27.305365896055672	34.0	20.0	34.0	15.6	34.0
245-249	27.841996602455716	34.0	20.0	34.0	15.6	34.0
250-254	27.864373084041812	34.0	20.0	34.0	15.6	34.0
255-259	26.94471570758797	34.0	20.0	34.0	15.6	34.0
260-264	27.60937491358995	34.0	20.0	34.0	11.2	34.0
265-269	26.750019526629263	34.0	20.0	34.0	13.4	34.0
270-274	26.953673520093417	34.0	20.0	34.0	15.6	34.0
275-279	25.325223825542594	28.4	20.0	34.0	9.0	34.0
280-284	25.944905071972038	34.0	20.0	34.0	9.0	34.0
285-289	26.03315749260027	31.2	20.0	34.0	13.4	34.0
290-294	24.757372713364695	20.0	20.0	34.0	9.0	34.0
295-299	24.83450049842639	20.0	20.0	34.0	9.0	34.0
300-301	21.798408369517084	20.0	14.5	27.0	9.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.8434644828553957
1101	2	-1.1827941637152612
1101	3	-0.31856703165961164
1101	4	-0.5958510766556273
1101	5	0.024588604979470574
1101	6	-0.5283163192687041
1101	7	-0.6927716836865763
1101	8	-0.5417982504213299
1101	9	-0.024861165865765145
1101	10-14	-0.650023481966393
1101	15-19	-0.45200649015148997
1101	20-24	-0.547865262483711
1101	25-29	-0.5318090331103917
1101	30-34	-0.740989561243758
1101	35-39	-0.895015206399723
1101	40-44	-0.5436046009574298
1101	45-49	-0.6429633112560182
1101	50-54	-0.6764303987748406
1101	55-59	-0.7479401041638774
1101	60-64	-0.9807868372845725
1101	65-69	-1.1123757659312794
1101	70-74	-1.2330071714933553
1101	75-79	-0.8503838017927414
1101	80-84	-1.1186990691702619
1101	85-89	-0.9939517778520752
1101	90-94	-0.9381657746666612
1101	95-99	-1.276648581585519
1101	100-104	-0.7108728393331241
1101	105-109	-1.0416420389776029
1101	110-114	-0.8482794848178692
1101	115-119	-1.107712388470489
1101	120-124	-1.3384017784504536
1101	125-129	-0.9890245148793895
1101	130-134	-0.9514905246016951
1101	135-139	-1.024388349054938
1101	140-144	-1.2718880657435072
1101	145-149	-1.0880344263496795
1101	150-154	-1.0613575684027126
1101	155-159	-1.1927071905745805
1101	160-164	-1.3592131662615294
1101	165-169	-1.121617241918969
1101	170-174	-1.6293461257194366
1101	175-179	-1.2171758937002473
1101	180-184	-1.2060757921093597
1101	185-189	-1.5827930257172653
1101	190-194	-1.6013679091285091
1101	195-199	-1.0614549968617233
1101	200-204	-1.784666230858086
1101	205-209	-1.5942823262648567
1101	210-214	-1.1040452493930744
1101	215-219	-2.225783903988191
1101	220-224	-1.8363328915282437
1101	225-229	-2.4639825951036016
1101	230-234	-2.6100693674903326
1101	235-239	-2.521954627414253
1101	240-244	-2.6917612901649868
1101	245-249	-2.868185233912712
1101	250-254	-2.8724022866290966
1101	255-259	-3.1523226150802373
1101	260-264	-3.200055557620807
1101	265-269	-3.1992825997637446
1101	270-274	-3.061961199640553
1101	275-279	-3.5186229629103245
1101	280-284	-3.4758414633314985
1101	285-289	-3.9897593514167937
1101	290-294	-4.114053731501798
1101	295-299	-4.2330333316170545
1101	300-301	-2.3617391014648845
1102	1	0.6356116387770783
1102	2	-0.3058228153542686
1102	3	-0.48273784557032684
1102	4	-0.34804479986743075
1102	5	-0.21071964221879114
1102	6	-0.5057598154142084
1102	7	-0.19143180483864342
1102	8	-0.16711257774209543
1102	9	-0.26558147272429267
1102	10-14	-0.5055537662308751
1102	15-19	-0.5126921177927883
1102	20-24	-0.5575108889239857
1102	25-29	-0.31409861208067724
1102	30-34	-0.8089338042685057
1102	35-39	-0.7704342469413881
1102	40-44	-0.6554112980997715
1102	45-49	-0.5762805827146877
1102	50-54	-0.500516990070544
1102	55-59	-0.2150107230387306
1102	60-64	-0.36158831860993246
1102	65-69	-0.7368221506844641
1102	70-74	-0.5585871314494035
1102	75-79	-0.8204276092806602
1102	80-84	-0.5353624741110181
1102	85-89	-0.2915280646029501
1102	90-94	-0.5891225133224616
1102	95-99	-0.5249714991956367
1102	100-104	-0.7970182111498367
1102	105-109	-0.881603182806149
1102	110-114	-0.8521741500155393
1102	115-119	-1.1403156577773608
1102	120-124	-1.0304096320926597
1102	125-129	-1.1451537042495765
1102	130-134	-1.11773356134335
1102	135-139	-1.2739515282580065
1102	140-144	-1.3716217115990368
1102	145-149	-1.24046594723373
1102	150-154	-1.7456504976956424
1102	155-159	-1.5122159141742095
1102	160-164	-1.8087448467023037
1102	165-169	-1.7152765623964719
1102	170-174	-1.8849108640114451
1102	175-179	-1.9719325511841816
1102	180-184	-1.6882796488586713
1102	185-189	-2.0000244560426204
1102	190-194	-1.8516594990609825
1102	195-199	-1.1945145152360368
1102	200-204	-2.3188111084339234
1102	205-209	-2.2516188772298236
1102	210-214	-1.2968350844411987
1102	215-219	-2.3743265659448944
1102	220-224	-2.355819049540095
1102	225-229	-2.2602034298062
1102	230-234	-2.321035988775712
1102	235-239	-2.4346864240435266
1102	240-244	-2.6531058279801094
1102	245-249	-3.527414768423146
1102	250-254	-3.204475115760747
1102	255-259	-3.6158057611476515
1102	260-264	-3.7500080817543697
1102	265-269	-4.084910072465124
1102	270-274	-3.8046131720094287
1102	275-279	-4.133767987982807
1102	280-284	-3.971289064487845
1102	285-289	-4.429451360032086
1102	290-294	-4.19498269212945
1102	295-299	-4.412766303140224
1102	300-301	-2.9212638110441986
1103	1	-0.3333587847387989
1103	2	-0.4272846868161366
1103	3	0.09641784722172986
1103	4	-0.10075421166775556
1103	5	-0.5058033463934066
1103	6	-0.025868494613796145
1103	7	-0.38442063419110895
1103	8	-0.2066109581495681
1103	9	0.196880307919308
1103	10-14	0.07891025459678502
1103	15-19	-0.1266539135727598
1103	20-24	-0.31538180588581355
1103	25-29	-0.11815062067814353
1103	30-34	-0.3151593968577373
1103	35-39	-0.2853526618597968
1103	40-44	-0.13066331880634152
1103	45-49	-0.2538056024797797
1103	50-54	-0.170473276877658
1103	55-59	-0.2058734514705165
1103	60-64	-0.2646057431743216
1103	65-69	-0.48674322408620085
1103	70-74	-0.7578312574891051
1103	75-79	-0.3491022495575038
1103	80-84	-0.47220528139581575
1103	85-89	-0.29064073830125636
1103	90-94	-0.23154523374618208
1103	95-99	-0.2717440970983276
1103	100-104	-0.6344929078912322
1103	105-109	-0.5061956671675922
1103	110-114	-0.21780995434833983
1103	115-119	-0.5535983207200594
1103	120-124	-0.45632173727950587
1103	125-129	-0.6466577906944835
1103	130-134	-0.36564455239208726
1103	135-139	-0.7980268317843233
1103	140-144	-0.7316701972232842
1103	145-149	-0.8498205892910384
1103	150-154	-0.9285264952401491
1103	155-159	-0.7989900087107351
1103	160-164	-0.9798682352842505
1103	165-169	-0.9808413260551596
1103	170-174	-1.331559114471851
1103	175-179	-1.0687223326553976
1103	180-184	-1.1395815879168403
1103	185-189	-1.2409693621675757
1103	190-194	-1.3884939096488544
1103	195-199	-0.9198058026345173
1103	200-204	-1.8315742127445702
1103	205-209	-1.8635377877838337
1103	210-214	-1.129862166704683
1103	215-219	-1.6319553679312406
1103	220-224	-1.8335692401689379
1103	225-229	-1.777374030948419
1103	230-234	-2.138354424529904
1103	235-239	-2.535245083261394
1103	240-244	-2.5301632431249423
1103	245-249	-2.780230051839883
1103	250-254	-2.9763852281202965
1103	255-259	-2.9764799184510196
1103	260-264	-3.0786216434010214
1103	265-269	-3.351024463374788
1103	270-274	-3.5682414815735335
1103	275-279	-3.376070844670963
1103	280-284	-3.667951147196881
1103	285-289	-4.184153021815899
1103	290-294	-4.280895595575249
1103	295-299	-4.387557822451548
1103	300-301	-3.8989375777106225
1104	1	-0.07784169908472904
1104	2	-0.5316426576124655
1104	3	-0.2837642993987757
1104	4	-0.03016110614314016
1104	5	-1.1335167289013057
1104	6	-0.9486067152972204
1104	7	-0.6147081548895912
1104	8	-1.0267148268835733
1104	9	-0.4418220855776056
1104	10-14	-0.4905131986622919
1104	15-19	-0.5317720830744932
1104	20-24	-0.7244424712478477
1104	25-29	-0.6624543990915086
1104	30-34	-0.8821727944190769
1104	35-39	-0.7234770032718103
1104	40-44	-0.845502999673414
1104	45-49	-0.8501717021335509
1104	50-54	-0.815848860198443
1104	55-59	-0.5873697633777866
1104	60-64	-0.815143150972002
1104	65-69	-0.9402551915707704
1104	70-74	-1.483655433313281
1104	75-79	-0.9716297220849803
1104	80-84	-0.9400707492612916
1104	85-89	-0.883714331374847
1104	90-94	-0.8786799172445043
1104	95-99	-1.6182597027048402
1104	100-104	-1.2833001612439396
1104	105-109	-1.642191489527054
1104	110-114	-1.326850913389297
1104	115-119	-1.535184915943013
1104	120-124	-1.3422479322966083
1104	125-129	-2.0555080313629084
1104	130-134	-1.882809205920374
1104	135-139	-1.6348279094944935
1104	140-144	-1.7361737800292225
1104	145-149	-2.253861023994453
1104	150-154	-1.8984553191368327
1104	155-159	-2.283616281483667
1104	160-164	-2.6253288687408727
1104	165-169	-2.434013936133848
1104	170-174	-2.8682043209312162
1104	175-179	-2.4003364799748432
1104	180-184	-2.230931582560327
1104	185-189	-3.3175996555515184
1104	190-194	-2.8295447047086206
1104	195-199	-2.397691050794169
1104	200-204	-3.0171297683001264
1104	205-209	-3.2224828499456635
1104	210-214	-2.6144118417278968
1104	215-219	-3.128924222377254
1104	220-224	-3.364137907809667
1104	225-229	-3.8342907183529
1104	230-234	-3.4302374347172204
1104	235-239	-3.722634449905051
1104	240-244	-4.006155587209644
1104	245-249	-4.666935013932331
1104	250-254	-4.9020616072226915
1104	255-259	-4.394244186204148
1104	260-264	-5.094092856355264
1104	265-269	-4.947433989974993
1104	270-274	-5.428123658404864
1104	275-279	-5.3172328687637815
1104	280-284	-5.522058899026259
1104	285-289	-5.599875159930452
1104	290-294	-5.961347206353295
1104	295-299	-5.649391605968248
1104	300-301	-3.748772472784122
1201	1	-1.550391751771766
1201	2	-0.7377242472556986
1201	3	-0.5849008340969561
1201	4	-0.23811684903039065
1201	5	-0.8492099397999979
1201	6	-0.7319124506577523
1201	7	-0.9036514034218825
1201	8	-0.6736439251825352
1201	9	-0.9981746371356373
1201	10-14	-0.5436172179306951
1201	15-19	-0.4145660014848431
1201	20-24	-0.7357114762154779
1201	25-29	-0.8555132580407729
1201	30-34	-0.8074670891654279
1201	35-39	-0.7990889255960631
1201	40-44	-0.9537402418832563
1201	45-49	-1.0911682398424176
1201	50-54	-0.9655282219326047
1201	55-59	-1.0240053196023844
1201	60-64	-1.0132888782210934
1201	65-69	-1.3122875023426914
1201	70-74	-1.4770747579013985
1201	75-79	-1.2123423335732113
1201	80-84	-1.1368668894092302
1201	85-89	-1.0785698913273656
1201	90-94	-1.1436946808742263
1201	95-99	-1.5757237068447445
1201	100-104	-1.5510256490525336
1201	105-109	-1.548287194376222
1201	110-114	-1.467721815340525
1201	115-119	-1.4250802007136514
1201	120-124	-1.4686367789070616
1201	125-129	-1.571192485413011
1201	130-134	-1.5465424256092781
1201	135-139	-1.7138407249339096
1201	140-144	-1.618159107720917
1201	145-149	-1.8018002039280958
1201	150-154	-2.237064779012975
1201	155-159	-2.264587413096958
1201	160-164	-2.077092030451144
1201	165-169	-2.5733575103804647
1201	170-174	-2.696942258286608
1201	175-179	-2.4820187315473667
1201	180-184	-2.3351494549528553
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2102	215-219	2.6985760330349393
2102	220-224	2.265413239343392
2102	225-229	2.896494813251042
2102	230-234	2.435474415240151
2102	235-239	2.543830295250462
2102	240-244	2.8552171533863486
2102	245-249	3.1236501089200743
2102	250-254	3.0210874961917042
2102	255-259	3.477550369775649
2102	260-264	2.949889329090766
2102	265-269	3.3306704518794454
2102	270-274	3.4449297294311307
2102	275-279	3.494512778574567
2102	280-284	3.713410219216641
2102	285-289	3.8991330251374947
2102	290-294	4.0330812294728595
2102	295-299	3.985066662388931
2102	300-301	2.7813078015068307
2103	1	0.8072103235722423
2103	2	0.2914401367473971
2103	3	0.2918111635827856
2103	4	0.11734220804157047
2103	5	0.7248530939404532
2103	6	0.5295196656130727
2103	7	0.6456782991336283
2103	8	0.8425737281318959
2103	9	0.43121049386239463
2103	10-14	0.2774729821917603
2103	15-19	0.3814070766703921
2103	20-24	0.5574753369713363
2103	25-29	0.26332319961921513
2103	30-34	0.5823737103265927
2103	35-39	0.5709441386847942
2103	40-44	0.4071304508458411
2103	45-49	0.5474942775811655
2103	50-54	0.5818930883205695
2103	55-59	0.5639019059154577
2103	60-64	0.7579719740936639
2103	65-69	1.0070410152732592
2103	70-74	1.1022857641323398
2103	75-79	0.7823217836364407
2103	80-84	0.8861975252370797
2103	85-89	0.736766073176355
2103	90-94	0.8733768755202576
2103	95-99	1.3251890314518562
2103	100-104	0.9778379631685183
2103	105-109	1.0208235190631747
2103	110-114	0.9974756450080591
2103	115-119	1.1075830830196765
2103	120-124	1.1902119155478559
2103	125-129	1.1614404048162967
2103	130-134	1.266894936091461
2103	135-139	1.2785702308267268
2103	140-144	1.3674610466825285
2103	145-149	1.3716250284146767
2103	150-154	1.3685868461570863
2103	155-159	1.5278268208685226
2103	160-164	1.5806437134554905
2103	165-169	1.479039676919772
2103	170-174	1.538619707980665
2103	175-179	1.5561871069354822
2103	180-184	1.5100635464076007
2103	185-189	1.61058040917764
2103	190-194	1.9221241958765596
2103	195-199	1.4416474255777096
2103	200-204	2.130528773882176
2103	205-209	2.0333129682976967
2103	210-214	2.537080634084269
2103	215-219	2.3128441060990035
2103	220-224	2.1120696152974254
2103	225-229	2.1558224866924256
2103	230-234	2.2100340584954488
2103	235-239	2.1023107000184957
2103	240-244	2.5539102328821066
2103	245-249	2.9775940245362307
2103	250-254	3.1465587250743035
2103	255-259	3.2228552321373733
2103	260-264	3.389626915918658
2103	265-269	3.263490964699958
2103	270-274	3.3283085134119226
2103	275-279	3.8573995528150817
2103	280-284	3.8771001896083916
2103	285-289	3.6348448009999856
2103	290-294	3.6695806109125932
2103	295-299	3.6588097428874953
2103	300-301	3.870169975118788
2104	1	0.8399819987714743
2104	2	0.23911811941717787
2104	3	0.6604428053144815
2104	4	0.05838467204740283
2104	5	0.4183600608208522
2104	6	0.403494515257691
2104	7	0.6086569097338881
2104	8	0.7153639595880605
2104	9	0.33886826346658694
2104	10-14	0.38398258339793045
2104	15-19	0.41651365332361934
2104	20-24	0.48481996973969643
2104	25-29	0.5005558924754823
2104	30-34	0.6031374620906789
2104	35-39	0.6400559238887311
2104	40-44	0.4995953743418724
2104	45-49	0.5037294969816699
2104	50-54	0.44987507165765805
2104	55-59	0.48168375053427326
2104	60-64	0.5988721387171552
2104	65-69	0.9299504222108439
2104	70-74	0.9937128075110522
2104	75-79	0.6681532994260877
2104	80-84	0.7709042530734891
2104	85-89	0.5886450373017951
2104	90-94	0.7134129756982546
2104	95-99	1.1489833941416592
2104	100-104	0.9231796195984501
2104	105-109	1.1290020478137954
2104	110-114	0.7915642408615611
2104	115-119	0.613470253250398
2104	120-124	1.0846735881093323
2104	125-129	1.1369537561413736
2104	130-134	1.0056850194010423
2104	135-139	1.082301561600982
2104	140-144	1.2876564973421338
2104	145-149	1.3842253938370064
2104	150-154	1.4056353065221643
2104	155-159	1.460709801910145
2104	160-164	1.5887433497094143
2104	165-169	1.4823737366277676
2104	170-174	1.7575914967062047
2104	175-179	1.5929053462032883
2104	180-184	1.549794005532263
2104	185-189	1.8211307217981165
2104	190-194	1.8706650209264204
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2104	230-234	2.4383844616333903
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2104	240-244	2.6762345200571254
2104	245-249	3.075708244158406
2104	250-254	2.9231214084140618
2104	255-259	2.8926425637314637
2104	260-264	3.0350276690117184
2104	265-269	3.249155300364297
2104	270-274	3.1836171470859433
2104	275-279	3.5282306448000185
2104	280-284	3.2442109591743638
2104	285-289	3.6100235169427926
2104	290-294	4.204244759820064
2104	295-299	3.3135951376929142
2104	300-301	2.5382603083295763
2201	1	1.5464233242696679
2201	2	0.47632531349873375
2201	3	0.4359515227152855
2201	4	0.2817751500879666
2201	5	0.6194997173050751
2201	6	0.35233138573508427
2201	7	0.6981701274827472
2201	8	0.8480270972048203
2201	9	0.5260940740958233
2201	10-14	0.618719442543501
2201	15-19	0.6550709518197237
2201	20-24	0.7075612968567242
2201	25-29	0.45968086016193155
2201	30-34	0.6991625276361049
2201	35-39	0.6812111468243529
2201	40-44	0.6582972907846383
2201	45-49	0.6954906599969988
2201	50-54	0.4955190402607954
2201	55-59	0.1025448076475115
2201	60-64	-0.673112530504639
2201	65-69	0.5504329998379234
2201	70-74	0.7034401336584395
2201	75-79	0.41353289427295437
2201	80-84	0.528849460963265
2201	85-89	0.3920620661406744
2201	90-94	0.49838649460451023
2201	95-99	0.8813106646225322
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2202	1	0.8097892437033458
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2202	9	0.36462432860450633
2202	10-14	0.3928792268010497
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2202	75-79	0.6436787157898323
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2204	1	1.0878118813298272
2204	2	0.7910938733991557
2204	3	0.5340159633912762
2204	4	0.31587507741051724
2204	5	0.29717938516075293
2204	6	0.47497250112515843
2204	7	0.7005760018259295
2204	8	0.4379842425680849
2204	9	0.3882909381054489
2204	10-14	0.5903702231996064
2204	15-19	0.5935524107151977
2204	20-24	0.4783499725398457
2204	25-29	0.4512034945943455
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2204	60-64	0.7070246672315932
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2204	70-74	1.0985154570208806
2204	75-79	0.775656664997328
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2204	85-89	0.8778017036105723
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2204	155-159	1.5201569397292438
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2301	1	-2.1304127945587688
2301	2	0.8285605317950342
2301	3	0.6095695890968713
2301	4	0.4620818884023876
2301	5	0.8104229804712233
2301	6	0.6822797401301841
2301	7	0.7217285357878467
2301	8	0.9813128201997401
2301	9	0.47780151437243035
2301	10-14	0.6521157724831497
2301	15-19	0.5389528201190856
2301	20-24	0.6096941819561366
2301	25-29	0.6583866718165936
2301	30-34	0.8659839279026116
2301	35-39	0.7907520844577149
2301	40-44	0.7729139549837001
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2301	50-54	0.8212382031316992
2301	55-59	0.6811219350837661
2301	60-64	0.6374111423791504
2301	65-69	0.9036830787849937
2301	70-74	1.1556690458884944
2301	75-79	0.8804197621730978
2301	80-84	0.9500627368844619
2301	85-89	0.8692216896593337
2301	90-94	0.7967060464850206
2301	95-99	1.3726490817622334
2301	100-104	1.00518834411573
2301	105-109	1.1759875706765435
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2301	115-119	1.0826233494112572
2301	120-124	1.1751322335498173
2301	125-129	1.163756446490531
2301	130-134	1.169857146132479
2301	135-139	1.2284667712848467
2301	140-144	1.4480033924915077
2301	145-149	1.5908939143187624
2301	150-154	1.6090086706751805
2301	155-159	1.6393416863711145
2301	160-164	1.7246702862220324
2301	165-169	1.775783423970264
2301	170-174	1.9521239008147333
2301	175-179	1.6894254735071783
2301	180-184	1.720115412869724
2301	185-189	2.149111346426526
2301	190-194	1.9856861799513617
2301	195-199	1.9254081093383952
2301	200-204	2.4062757239881023
2301	205-209	2.394610360364318
2301	210-214	-3.034371970626246
2301	215-219	1.9103838133553097
2301	220-224	2.3937355797211914
2301	225-229	2.6573796339985627
2301	230-234	2.68245606373479
2301	235-239	2.8386957196811053
2301	240-244	3.072981128541631
2301	245-249	3.100917189665413
2301	250-254	3.264482941326282
2301	255-259	3.1477367051793728
2301	260-264	3.75100734151313
2301	265-269	3.708641466372196
2301	270-274	3.4655094395760564
2301	275-279	3.125873219616505
2301	280-284	3.7312204171077354
2301	285-289	4.098003471770578
2301	290-294	4.009326634467154
2301	295-299	4.5680683102530395
2301	300-301	4.413764344549438
2302	1	0.9622615369684766
2302	2	0.3661139435390126
2302	3	0.4081952003034317
2302	4	0.24543944974330145
2302	5	0.5701163860480705
2302	6	0.6732316805733376
2302	7	0.5838227166609329
2302	8	0.16272333074994094
2302	9	0.13427479748771276
2302	10-14	0.3761614092392378
2302	15-19	0.3084026240924729
2302	20-24	0.3606637427684305
2302	25-29	0.2956079396021636
2302	30-34	0.5510374497738937
2302	35-39	0.4804221430454447
2302	40-44	0.37894012123188503
2302	45-49	0.5247419369373247
2302	50-54	0.451786637121387
2302	55-59	0.6192770502017311
2302	60-64	0.6014143237153107
2302	65-69	0.8621801713589292
2302	70-74	0.927387726425529
2302	75-79	0.6891936574340463
2302	80-84	0.734108260135109
2302	85-89	0.8844844263891289
2302	90-94	0.792879248227699
2302	95-99	1.1200537229998915
2302	100-104	0.9950291905636703
2302	105-109	1.2220496316705898
2302	110-114	0.792730230104894
2302	115-119	0.9204752210214693
2302	120-124	1.0792968996830012
2302	125-129	1.2031589538258132
2302	130-134	1.0501896792524263
2302	135-139	1.133337403453421
2302	140-144	1.2813403960542118
2302	145-149	1.408424945849653
2302	150-154	1.5282125513999674
2302	155-159	1.5648125095983936
2302	160-164	1.3097822850806473
2302	165-169	1.5925012153813078
2302	170-174	1.9118720817249866
2302	175-179	1.5840493609605275
2302	180-184	1.6585808162576114
2302	185-189	1.9147946654044112
2302	190-194	1.9633275810423747
2302	195-199	1.6345812359533802
2302	200-204	1.992228925251375
2302	205-209	2.2430384396063516
2302	210-214	2.532192096861209
2302	215-219	2.2617914020946017
2302	220-224	2.2727927472638534
2302	225-229	2.3598888139251244
2302	230-234	2.55225230536486
2302	235-239	2.510178458378199
2302	240-244	2.9227247368039464
2302	245-249	3.1907734658462665
2302	250-254	3.0779850357939935
2302	255-259	3.5279295406553253
2302	260-264	3.465683060132225
2302	265-269	3.9528022348251817
2302	270-274	3.742430572033083
2302	275-279	3.4639600316921957
2302	280-284	4.049456730844554
2302	285-289	3.9174495211711253
2302	290-294	4.378769072716469
2302	295-299	4.68069348454636
2302	300-301	4.271769247168837
2303	1	1.0912970192273477
2303	2	0.5280329840187719
2303	3	0.4161412272899341
2303	4	0.21745344274679468
2303	5	0.5857085899461154
2303	6	0.41318670306209526
2303	7	0.623852701668433
2303	8	0.46122408037512486
2303	9	0.20556415281004803
2303	10-14	0.28590153917426875
2303	15-19	0.49697446209277985
2303	20-24	0.648368708729258
2303	25-29	0.5233621892784726
2303	30-34	0.698437524952439
2303	35-39	0.8107901002771953
2303	40-44	0.5638041733556776
2303	45-49	0.7670925195710794
2303	50-54	0.5729143153572025
2303	55-59	0.7175184370793701
2303	60-64	0.8453097748336802
2303	65-69	1.0110685017602066
2303	70-74	1.1211784611159885
2303	75-79	0.9070306348990727
2303	80-84	0.8961707548992877
2303	85-89	0.9588950823328233
2303	90-94	1.0565736438044837
2303	95-99	1.2841993246176884
2303	100-104	1.1182715228858768
2303	105-109	1.240743598640595
2303	110-114	1.076164675672807
2303	115-119	1.1895786925415237
2303	120-124	1.1921246718434375
2303	125-129	1.1952266990229887
2303	130-134	1.2852587726127211
2303	135-139	1.4552180910166221
2303	140-144	1.4255180165462917
2303	145-149	1.490983085384535
2303	150-154	1.6584451095394925
2303	155-159	1.581091579365836
2303	160-164	1.6295497269411108
2303	165-169	1.7535477270092095
2303	170-174	2.241523244515683
2303	175-179	1.8561423842163407
2303	180-184	1.7754412813738867
2303	185-189	2.237469084009067
2303	190-194	2.1900717670888845
2303	195-199	1.6898137940929168
2303	200-204	2.133507995018821
2303	205-209	2.459317509373797
2303	210-214	3.003122329419355
2303	215-219	2.5687681462806395
2303	220-224	2.393722979821991
2303	225-229	2.6348888139251194
2303	230-234	2.3749267239695158
2303	235-239	2.6386668304712266
2303	240-244	2.3017945042458052
2303	245-249	2.9977502100323115
2303	250-254	3.0117059660265575
2303	255-259	2.9029295406553253
2303	260-264	3.4662644554810633
2303	265-269	3.251639444127509
2303	270-274	3.414260865612313
2303	275-279	3.068324734383907
2303	280-284	3.589706917297871
2303	285-289	3.844714081480298
2303	290-294	3.9137216059241915
2303	295-299	4.111573232178145
2303	300-301	0.6375964054439791
2304	1	1.1960491207885084
2304	2	0.7745932859573585
2304	3	0.7060537337232873
2304	4	0.2985651257112849
2304	5	0.5280657695652664
2304	6	0.45826304548789665
2304	7	0.4770522213115953
2304	8	0.5569694912517775
2304	9	0.6615514576650838
2304	10-14	0.4405438448870811
2304	15-19	0.5293933227729823
2304	20-24	0.5368783220459576
2304	25-29	0.5202239897860252
2304	30-34	0.7715176574312608
2304	35-39	0.792695558576483
2304	40-44	0.6680770453071574
2304	45-49	0.7740605612072855
2304	50-54	0.6728119453627883
2304	55-59	0.712748821778618
2304	60-64	0.7564045897763094
2304	65-69	0.9799305499009989
2304	70-74	1.1103252129061687
2304	75-79	0.8476535319743945
2304	80-84	0.8829553291379213
2304	85-89	0.9834072038063866
2304	90-94	0.9159075848056872
2304	95-99	1.2353814322795813
2304	100-104	0.922054931424583
2304	105-109	1.3417727551834595
2304	110-114	1.0524749845933172
2304	115-119	1.1190302718542569
2304	120-124	1.306543276494594
2304	125-129	1.1479740089848107
2304	130-134	1.316570817041736
2304	135-139	1.3514555085799635
2304	140-144	1.425712393498742
2304	145-149	1.5724322349853637
2304	150-154	1.5552864840622362
2304	155-159	1.7151058105355723
2304	160-164	1.7041904766807896
2304	165-169	1.5198892750828001
2304	170-174	2.0839946086253747
2304	175-179	1.948448875018137
2304	180-184	1.6836606496487931
2304	185-189	1.9860286119507542
2304	190-194	1.826342159313807
2304	195-199	1.9130366333848308
2304	200-204	2.3466110842239587
2304	205-209	2.431304666610856
2304	210-214	2.7601071483964645
2304	215-219	2.6836268010641007
2304	220-224	2.5280601194961356
2304	225-229	2.6218927131035166
2304	230-234	2.560886200365559
2304	235-239	2.6278466104461593
2304	240-244	2.567937799051542
2304	245-249	3.5308201167305384
2304	250-254	3.399232076596114
2304	255-259	3.029151515310666
2304	260-264	3.5210374675963685
2304	265-269	3.9269179568694668
2304	270-274	3.7663165210652565
2304	275-279	3.9058556977244727
2304	280-284	4.226525660239062
2304	285-289	4.345979585278382
2304	290-294	4.653916172744484
2304	295-299	4.621181383664695
2304	300-301	2.49526879855625
2401	1	0.14651580752772375
2401	2	0.47052859500290367
2401	3	-0.036059905573026185
2401	4	0.023078007985787963
2401	5	0.6064777442738531
2401	6	0.3389460856186517
2401	7	0.5318158670597981
2401	8	0.5390504409700441
2401	9	0.35580927520562255
2401	10-14	0.2791192674859104
2401	15-19	0.3562228638285134
2401	20-24	0.3941900343776723
2401	25-29	0.42999043135340287
2401	30-34	0.49901389382852557
2401	35-39	0.6044500426403161
2401	40-44	0.46697420217412144
2401	45-49	0.5083028942108498
2401	50-54	0.3861707995070489
2401	55-59	0.4995577167848566
2401	60-64	0.6128715345911431
2401	65-69	0.856505026222564
2401	70-74	0.846993456701604
2401	75-79	0.5184247479406707
2401	80-84	0.5460599788002796
2401	85-89	0.5262486163402613
2401	90-94	0.7324674368423345
2401	95-99	1.1337512546375308
2401	100-104	0.8515400705703513
2401	105-109	0.9313826308770352
2401	110-114	0.9909927703045369
2401	115-119	0.9961432659615355
2401	120-124	1.2215865041602996
2401	125-129	1.044246536835466
2401	130-134	1.1777760301691842
2401	135-139	1.1602713716278394
2401	140-144	1.2269924485548742
2401	145-149	1.5001228883468087
2401	150-154	1.503361670091735
2401	155-159	1.367551937250699
2401	160-164	1.5248382464773336
2401	165-169	1.631045883969204
2401	170-174	1.635156379024103
2401	175-179	1.62182665470759
2401	180-184	1.7807693424957947
2401	185-189	1.7502765365926614
2401	190-194	1.9908877311663637
2401	195-199	1.6504481349547966
2401	200-204	1.9266016214513613
2401	205-209	2.496190038024686
2401	210-214	2.1813962386747647
2401	215-219	2.291393975648649
2401	220-224	2.3775829758008094
2401	225-229	2.7765666505312474
2401	230-234	2.5520646503819506
2401	235-239	3.021503101498638
2401	240-244	3.4470176796361933
2401	245-249	3.4799012052739116
2401	250-254	3.546892950813092
2401	255-259	3.634723656331218
2401	260-264	3.7076239488126106
2401	265-269	3.9922650724366022
2401	270-274	3.7392220069235087
2401	275-279	3.830843953681338
2401	280-284	3.7978214517077653
2401	285-289	4.245069330393722
2401	290-294	4.052011444002936
2401	295-299	4.460902116306254
2401	300-301	3.922109237523518
2402	1	-0.04063701375616091
2402	2	0.22118640730712968
2402	3	0.4255865046512568
2402	4	-0.029922869097283922
2402	5	0.48026131358430035
2402	6	0.41526066608058443
2402	7	0.3403444557913673
2402	8	0.493158113358632
2402	9	-0.0512324488891025
2402	10-14	0.40224836576097545
2402	15-19	0.49390987046928814
2402	20-24	0.6470405543626327
2402	25-29	0.40401373670361096
2402	30-34	0.6107475947014294
2402	35-39	0.37549460681355384
2402	40-44	0.5047372226811646
2402	45-49	0.5763361398358811
2402	50-54	0.40540982552717963
2402	55-59	0.5068132820857798
2402	60-64	0.5457621498022576
2402	65-69	0.8842091568661772
2402	70-74	1.045068885845815
2402	75-79	0.6787588748253555
2402	80-84	0.7868618833235175
2402	85-89	0.8328902234905726
2402	90-94	0.6844734511262587
2402	95-99	1.0968653172027913
2402	100-104	0.9324204949114971
2402	105-109	1.2974119505111723
2402	110-114	0.9144693605396768
2402	115-119	0.9448230471084287
2402	120-124	1.0253838562047406
2402	125-129	1.0164922871591457
2402	130-134	1.0293201140350305
2402	135-139	1.2121779831635742
2402	140-144	1.1804084782921649
2402	145-149	1.3771391352665674
2402	150-154	1.3494461206618453
2402	155-159	1.3002236993523546
2402	160-164	1.6344435590948017
2402	165-169	1.5777910704537739
2402	170-174	1.754364498307396
2402	175-179	1.6168114101684807
2402	180-184	1.5146189018659726
2402	185-189	1.841651760112871
2402	190-194	1.6621647903447005
2402	195-199	1.5915579801394308
2402	200-204	2.2102521810653286
2402	205-209	2.3633872768156508
2402	210-214	2.7949827945356347
2402	215-219	2.4257448904666994
2402	220-224	2.1274439100545486
2402	225-229	2.231400441832104
2402	230-234	2.432484863504392
2402	235-239	2.6590156676805243
2402	240-244	3.2552828763388284
2402	245-249	3.3983316053811414
2402	250-254	3.4308920125381803
2402	255-259	3.594790005771607
2402	260-264	3.4331249205973364
2402	265-269	3.349895258081002
2402	270-274	3.8656863859865744
2402	275-279	4.189541427041036
2402	280-284	3.977945102937575
2402	285-289	4.1360541723339175
2402	290-294	4.395048142483912
2402	295-299	4.5411653809461185
2402	300-301	3.3311390398343086
2404	1	0.7129861746496395
2404	2	0.4646646681766953
2404	3	0.1994995481295163
2404	4	0.019352493221560962
2404	5	0.43098595126545547
2404	6	0.4471447240515971
2404	7	0.7751270644870232
2404	8	0.5569262293006645
2404	9	0.4009414641543785
2404	10-14	0.6155816990943066
2404	15-19	0.5599968269910178
2404	20-24	0.653417365956841
2404	25-29	0.4347383743847715
2404	30-34	0.8130664352811436
2404	35-39	0.716943882175876
2404	40-44	0.5737227299275318
2404	45-49	0.63198831374892
2404	50-54	0.6146851878460211
2404	55-59	0.6737698038249036
2404	60-64	0.9341679469037132
2404	65-69	0.9555135046922629
2404	70-74	1.2549239583095826
2404	75-79	0.7616574255499913
2404	80-84	0.941064781874239
2404	85-89	0.923904716244202
2404	90-94	0.8914299728653887
2404	95-99	1.2343863822516639
2404	100-104	1.0316436159091324
2404	105-109	1.2296970870126849
2404	110-114	0.9750018849751356
2404	115-119	1.317485669285702
2404	120-124	1.2386362997504108
2404	125-129	1.4882499548369381
2404	130-134	1.4009564470313194
2404	135-139	1.5755669282259213
2404	140-144	1.4737738304997876
2404	145-149	1.6677272714310547
2404	150-154	1.6229799932604294
2404	155-159	1.903765997970492
2404	160-164	2.0004799594992484
2404	165-169	1.7925012153813107
2404	170-174	2.0694302212598714
2404	175-179	1.931723779565175
2404	180-184	1.9521854674203993
2404	185-189	2.2607248979625503
2404	190-194	2.2563508368563383
2404	195-199	1.8456277475812932
2404	200-204	2.5160661345537036
2404	205-209	2.9773407651877477
2404	210-214	3.3339362829077217
2404	215-219	3.0687681462806395
2404	220-224	2.6525795124682823
2404	225-229	2.789250126556489
2404	230-234	2.929769425169596
2404	235-239	3.1754557653142683
2404	240-244	3.676340236159831
2404	245-249	3.973740107652482
2404	250-254	4.004602094577638
2404	255-259	4.081226374338968
2404	260-264	4.007807949955264
2404	265-269	4.493430413006752
2404	270-274	4.570755844556267
2404	275-279	4.917744201418838
2404	280-284	4.570543362151504
2404	285-289	4.630032820504731
2404	290-294	5.035079422233672
2404	295-299	5.0192994204566155
2404	300-301	3.521077231402682
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	6.0
16	295.0
17	708.0
18	781.0
19	715.0
20	755.0
21	813.0
22	1021.0
23	1248.0
24	1627.0
25	2261.0
26	3331.0
27	5111.0
28	7601.0
29	10376.0
30	12612.0
31	14238.0
32	19632.0
33	26167.0
34	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	99.40540985556034	0.15642294569105095	0.06037376851233545	0.377793430236281
2	0.45737703418435954	98.85472790640236	0.09787868531545293	0.5900163740978238
3	0.4473147394323036	0.4546327719792534	0.1866098299472187	98.91144265864122
4	0.1664852404431069	0.29546556408309627	99.21422625527127	0.32382294020252655
5	0.2405803199809731	98.94986232951271	0.4162131011077672	0.3933442493985492
6	0.23783605777586694	0.6686852239775337	0.17105901078495045	98.92241970746166
7	0.4024917900822364	31.82978256295795	37.364959430657066	30.402766216302744
8	34.05720871943578	33.63733660205453	0.49762621319258316	31.8078284653171
9	99.22337379595496	0.1829508136737438	0.354924578527063	0.23875081184423566
10-14	39.93889442823297	19.95700655878667	39.89022951179575	0.21386950118460651
15-19	0.27279214007354957	39.887663062370784	39.73306255374426	20.106482243811406
20-24	19.848007129354972	0.46187094330369477	39.65301121921851	40.03711070812281
25-29	20.089496513479382	0.34682186716447955	43.16715166822233	36.39652995113381
30-34	0.4078248183733501	35.692725598695255	0.6512750016931685	63.248174581238224
35-39	19.895361757981046	0.6478564354532524	55.774416352562774	23.682365454002927
40-44	38.48028311610799	17.78959259035494	22.56433916079435	21.165785132742716
45-49	0.8223211392013359	19.914783286977446	20.092943711925763	59.169951861895456
50-54	39.42324068630952	0.8294097071518961	21.908576305143658	37.83877330139492
55-59	36.41580698103807	23.16608540195188	39.69238152457431	0.7257260924357434
60-64	20.625349478875435	35.938620051149954	3.4387804676829434	39.997250002291665
65-69	37.994488496894505	39.836858039322244	1.7509395442086073	20.41771391957465
70-74	40.28901479244284	19.324419655651333	1.7244990189810632	38.66206653292476
75-79	0.7038850559929446	0.2719263594021295	39.85889226755349	59.16529631705144
80-84	20.05336145957557	21.15219592328739	0.6925597146705426	58.10188290246649
85-89	59.6008769753539	0.28852709673743854	39.62083496433842	0.48976096357023796
90-94	0.3815692494885759	0.30841676331494716	76.45500846399997	22.855005523196507
95-99	0.7248947955752554	39.34893674130099	20.292090502462585	39.63407796066117
100-104	0.2514369798133076	18.9850554099416	58.979721340874605	21.783786269370488
105-109	20.135588854853694	39.354987075824894	0.6564193135929206	39.85300475572849
110-114	0.29678231302530284	20.121767225517758	43.00197241562035	36.57947804583658
115-119	20.010453647826175	0.7080137885087172	19.808742415123927	59.47279014854118
120-124	0.4729146378953918	55.70728259088742	3.5741964224900453	40.245606348727144
125-129	55.94249165985463	26.682389589731127	16.808062508745188	0.5670562416690601
130-134	0.9693094958817237	19.302774795743435	39.49310121955486	40.23481448881998
135-139	0.7730933311720367	2.1179478488958967	39.37047325027078	57.73848556966129
140-144	0.4001650773062921	0.6054062817810661	55.838504281692636	43.15592435922001
145-149	20.131844720313744	20.107517766048417	19.84839884410108	39.912238669536755
150-154	0.6576727566389549	38.22264260178071	21.16426700002028	39.95541764156005
155-159	20.612332584037603	67.76596156647679	8.704431417312234	2.9172744321733695
160-164	20.35568785191169	37.1937803499337	20.712924899071776	21.73760689908284
165-169	16.42926973235202	5.9266227657572905	41.966133584195674	35.67797391769501
170-174	23.641775181339597	35.214664381046575	20.683877705793456	20.459682731820372
175-179	16.687216440803727	4.895952925020396	40.43881766197713	37.97801297219874
180-184	40.6017563842926	2.3353228191500164	37.46393172365285	19.598989072904534
185-189	22.31971564418594	37.38503439043531	18.559940913077597	21.735309052301158
190-194	53.715100514949796	20.059400234941226	4.7337332752137	21.491765974895273
195-199	17.30225323335384	2.0206498667124815	60.534701862700004	20.14239503723368
200-204	1.011152059083365	38.73520051158269	18.636173775472862	41.61747365386108
205-209	18.946723957109853	38.93277744586479	5.019634246268753	37.10086435075661
210-214	2.4236250624864413	23.63656942361563	71.04873470850664	2.891070805391283
215-219	1.4931930417760377	9.030342127508119	32.60684996514956	56.869614865566284
220-224	34.213650071778055	4.070947136482171	20.119741376432316	41.59566141530746
225-229	21.661970053055025	3.6306865223896665	17.71143114912955	56.99591227542575
230-234	16.21241160040411	2.2610610922921497	59.59859897783324	21.927928329470493
235-239	19.857515552879793	18.714369597371807	0.4688161972930185	60.95929865245539
240-244	3.37314132270134	55.475799073171814	21.556007646704405	19.595051957422434
245-249	2.481680926206417	34.95156470382458	23.076822882746765	39.489931487222236
250-254	36.33755032269838	42.43641929561257	2.8461885094798465	18.37984187220921
255-259	20.36298175028093	19.861501161921765	20.264400096159044	39.51111699163826
260-264	20.09044542038472	18.086473281303906	18.230440012308037	43.592641286003335
265-269	2.26618107421463	19.961620524805724	20.029941964110712	57.742256436868935
270-274	17.661445672828098	21.601760800880403	59.07259006261082	1.664203463680679
275-279	22.445773771074975	18.111232810027992	0.9209799571245354	58.522013461772495
280-284	57.58333833413346	0.5038931228996639	19.836923907825252	22.07584463514162
285-289	41.11762352883732	2.423533344476015	19.873161222715297	36.58568190397136
290-294	21.977034955975505	36.41718198046201	19.695971137406133	21.90981192615635
295-299	36.637920462756654	19.36416239498454	40.5098850843614	3.488032057897408
300-301	12.822655039771638	1.3907019650845038	0.44471132926043894	85.34193166588342
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.25
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	2.0
17	3.0
18	2.0
19	2.0
20	3.0
21	3.0
22	2.5
23	2.3333333333333335
24	2.916666666666667
25	2.5
26	4.0
27	6.0
28	5.833333333333334
29	5.333333333333334
30	7.166666666666666
31	7.333333333333333
32	7.083333333333333
33	6.25
34	6.5
35	7.583333333333333
36	9.166666666666666
37	9.583333333333332
38	10.0
39	12.75
40	15.083333333333334
41	16.916666666666668
42	19.833333333333325
43	25.58333333333332
44	42.833333333333364
45	81.66666666666663
46	108.6666666666666
47	867.0000000000003
48	1169.6666666666667
49	275.3333333333334
50	290.5833333333334
51	831.8333333333362
52	7456.999999999991
53	20087.499999999993
54	4559.666666666669
55	825.6666666666667
56	294.1666666666667
57	103.33333333333331
58	27.25
59	10.416666666666666
60	6.416666666666666
61	3.5833333333333335
62	2.5
63	1.75
64	1.8333333333333333
65	0.5833333333333333
66	0.5833333333333333
67	0.25
68	0.0
69	0.25
70	0.5833333333333333
71	0.0
72	0.25
73	0.3333333333333333
74	0.3333333333333333
75	0.25
76	0.3333333333333333
77	0.25
78	0.25
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.25
87	0.5
88	0.0
89	0.25
90	0.3333333333333333
91	0.25
92	0.9166666666666665
93	1.3333333333333333
94	1.5833333333333333
95	0.75
96	0.8333333333333333
97	5.999999999999999
98	13.916666666666668
99	23.666666666666664
100	56.00000000000005
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	3.6875263888607205E-4
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	1.8464629156388022E-4
190-194	0.0
195-199	0.0
200-204	0.0
205-209	1.8496665051291253E-4
210-214	1.8247377622377623
215-219	1.8537433566472456E-4
220-224	0.0
225-229	1.8563655703590396E-4
230-234	0.0
235-239	1.8581661758011018E-4
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	1.8752965312640062E-4
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
10-19	19.0
20-29	30.0
30-39	21.0
40-49	37.0
50-59	117.0
60-69	222.0
70-79	27.0
80-89	24.0
90-99	74.0
100-109	43.0
110-119	51.0
120-129	30.0
130-139	61.0
140-149	74.0
150-159	41.0
160-169	43.0
170-179	66.0
180-189	46.0
190-199	55.0
200-209	169.0
210-219	208.0
220-229	156.0
230-239	129.0
240-249	136.0
250-259	156.0
260-269	195.0
270-279	365.0
280-289	403.0
290-299	568.0
300-302	105753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.806685022731639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.28074183577476	4.851855578627686
2	8.762263136675177	1.1928393051528097
3	3.951081843838194	0.8068130883012102
4	2.755006047574251	0.7500983360623497
5	1.6395645746539445	0.5579999817049186
6	1.3573444429512163	0.5543409654314437
7	1.3707834968418222	0.6531344048152654
8	1.2901491734981858	0.7025311245071763
9	0.8197822873269722	0.5021999835344267
>10	5.886305604085472	7.2018587802669245
>50	0.3359763472651526	1.6145409306707892
>100	0.18814675446848542	2.5219769664925584
>500	0.2419029700309098	10.559006211180124
>1k	0.0	0.0
>5k	0.1209514850154549	67.53080434325231
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	9472	8.664550535588507	No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	9288	8.496235787008663	No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	9175	8.392868577282997	No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	8133	7.439694838042792	No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	8034	7.3491341852742895	No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7821	7.154291568711751	No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7579	6.932921084166521	No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7328	6.703317813005974	No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6994	6.397789954170821	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	811	0.7418655494470312	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	800	0.7318032546949752	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	788	0.7208262058745506	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	702	0.6421573559948408	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	694	0.6348393234478911	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	691	0.6320950612427848	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	671	0.6137999798754106	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	668	0.6110557176703044	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	640	0.5854426037559802	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	632	0.5781245712090304	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	622	0.5689770305253433	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	618	0.5653180142518685	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	564	0.5159212945599576	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	560	0.5122622782864827	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	537	0.49122293471400214	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	529	0.48390490216705234	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	513	0.4692688370731529	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	503	0.4601212963894657	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	297	0.27168195830550956	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	260	0.23783605777586694	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	257	0.23509179557076082	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	249	0.22777376302381105	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	244	0.22319999268196744	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	234	0.21405245199828024	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	228	0.20856392758806797	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	214	0.19575737063090587	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	211	0.19301310842579972	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	127	0.11617376668282733	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	117	0.10702622599914012	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	110	0.1006229475205591	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
