FastQCFastQC Report
Fri 1 Nov 2024
IVMS2_cleaned_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIVMS2_cleaned_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109319
Sequences flagged as poor quality0
Sequence length15-301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG94728.664550535588507No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG92888.496235787008663No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG91758.392868577282997No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG81337.439694838042792No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG80347.3491341852742895No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG78217.154291568711751No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG75796.932921084166521No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG73286.703317813005974No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG69946.397789954170821No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8110.7418655494470312No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8000.7318032546949752No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7880.7208262058745506No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7020.6421573559948408No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6940.6348393234478911No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6910.6320950612427848No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6710.6137999798754106No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6680.6110557176703044No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG6400.5854426037559802No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG6320.5781245712090304No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG6220.5689770305253433No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6180.5653180142518685No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG5640.5159212945599576No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG5600.5122622782864827No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG5370.49122293471400214No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG5290.48390490216705234No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG5130.4692688370731529No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG5030.4601212963894657No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2970.27168195830550956No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2600.23783605777586694No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2570.23509179557076082No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2490.22777376302381105No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2440.22319999268196744No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2340.21405245199828024No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2280.20856392758806797No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2140.19575737063090587No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2110.19301310842579972No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1270.11617376668282733No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1170.10702622599914012No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1100.1006229475205591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)