##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IPMS2_cleaned_R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	243196
Sequences flagged as poor quality	0
Sequence length	15-301
%GC	52
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.032599220381915	34.0	34.0	34.0	20.0	34.0
2	32.48720373690357	34.0	34.0	34.0	34.0	34.0
3	32.56954884126384	34.0	34.0	34.0	34.0	34.0
4	33.292225201072384	34.0	34.0	34.0	34.0	34.0
5	32.41308656392375	34.0	34.0	34.0	34.0	34.0
6	32.76088422506949	34.0	34.0	34.0	34.0	34.0
7	32.43233030148522	34.0	34.0	34.0	34.0	34.0
8	32.46558742742479	34.0	34.0	34.0	34.0	34.0
9	32.87375203539532	34.0	34.0	34.0	34.0	34.0
10-14	32.965394167667235	34.0	34.0	34.0	34.0	34.0
15-19	32.92634344344156	34.0	34.0	34.0	34.0	34.0
20-24	32.7767598455141	34.0	34.0	34.0	34.0	34.0
25-29	32.91674678524544	34.0	34.0	34.0	34.0	34.0
30-34	32.62734287636256	34.0	34.0	34.0	34.0	34.0
35-39	32.615651901606576	34.0	34.0	34.0	34.0	34.0
40-44	32.68865145201797	34.0	34.0	34.0	34.0	34.0
45-49	32.627475462205744	34.0	34.0	34.0	34.0	34.0
50-54	32.56285274429079	34.0	34.0	34.0	31.2	34.0
55-59	32.51857675889392	34.0	34.0	34.0	31.2	34.0
60-64	32.4373579820117	34.0	34.0	34.0	34.0	34.0
65-69	31.966713029864458	34.0	34.0	34.0	22.8	34.0
70-74	31.88947603070462	34.0	34.0	34.0	22.8	34.0
75-79	32.55463456897507	34.0	34.0	34.0	34.0	34.0
80-84	32.33407009558031	34.0	34.0	34.0	31.2	34.0
85-89	32.46850795885424	34.0	34.0	34.0	34.0	34.0
90-94	32.365699585046805	34.0	34.0	34.0	28.4	34.0
95-99	32.07315902872078	34.0	34.0	34.0	20.0	34.0
100-104	32.05266817493386	34.0	34.0	34.0	25.6	34.0
105-109	31.994330899862035	34.0	34.0	34.0	20.0	34.0
110-114	32.2964998683088	34.0	34.0	34.0	28.4	34.0
115-119	32.00792916617885	34.0	34.0	34.0	20.0	34.0
120-124	31.72484368943489	34.0	34.0	34.0	20.0	34.0
125-129	31.144250045735113	34.0	34.0	34.0	17.8	34.0
130-134	31.646576585262352	34.0	34.0	34.0	20.0	34.0
135-139	31.489214832552683	34.0	34.0	34.0	20.0	34.0
140-144	31.67722299037475	34.0	34.0	34.0	20.0	34.0
145-149	31.3033684467855	34.0	34.0	34.0	20.0	34.0
150-154	31.267302660243082	34.0	34.0	34.0	20.0	34.0
155-159	31.126498234842813	34.0	34.0	34.0	20.0	34.0
160-164	31.159198585641356	34.0	34.0	34.0	20.0	34.0
165-169	31.219816051288962	34.0	34.0	34.0	20.0	34.0
170-174	30.530610050855056	34.0	34.0	34.0	20.0	34.0
175-179	31.00791863257468	34.0	34.0	34.0	20.0	34.0
180-184	31.22364701066537	34.0	34.0	34.0	20.0	34.0
185-189	30.509655435777614	34.0	34.0	34.0	20.0	34.0
190-194	30.351446747923802	34.0	31.2	34.0	17.8	34.0
195-199	30.899827124173292	34.0	34.0	34.0	20.0	34.0
200-204	29.870899704102648	34.0	28.4	34.0	20.0	34.0
205-209	29.722036548065308	34.0	31.2	34.0	20.0	34.0
210-214	29.396222741592993	34.0	31.2	34.0	17.8	34.0
215-219	29.413190498827912	34.0	20.0	34.0	20.0	34.0
220-224	29.660491703110818	34.0	25.6	34.0	20.0	34.0
225-229	28.80636328810968	34.0	20.0	34.0	15.6	34.0
230-234	29.54579764297973	34.0	22.8	34.0	20.0	34.0
235-239	28.62807147015413	34.0	20.0	34.0	17.8	34.0
240-244	27.719758829392134	34.0	20.0	34.0	13.4	34.0
245-249	28.035504030119824	34.0	20.0	34.0	15.6	34.0
250-254	28.01481949045308	34.0	20.0	34.0	17.8	34.0
255-259	27.034564956464305	34.0	20.0	34.0	13.4	34.0
260-264	27.472789443075307	34.0	20.0	34.0	13.4	34.0
265-269	26.903084981621173	34.0	20.0	34.0	11.2	34.0
270-274	26.90781353907505	34.0	20.0	34.0	13.4	34.0
275-279	25.38771769027351	28.4	20.0	34.0	9.0	34.0
280-284	25.970125147002953	34.0	20.0	34.0	9.0	34.0
285-289	25.99807972465522	28.4	20.0	34.0	11.2	34.0
290-294	24.735485062470246	22.8	20.0	34.0	9.0	34.0
295-299	24.876276735553496	20.0	20.0	34.0	9.0	34.0
300-301	21.82346316916706	20.0	14.5	27.0	9.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.100537079298185
1101	2	-0.6618360006037847
1101	3	-0.261198236815801
1101	4	-0.5081292668430777
1101	5	-0.5242593995451976
1101	6	-0.6493085442834285
1101	7	-0.7588154041893596
1101	8	-0.6236205720954935
1101	9	-0.4093816019484109
1101	10-14	-0.5718146233484092
1101	15-19	-0.43891588746983246
1101	20-24	-0.4814409524605452
1101	25-29	-0.43251613746826223
1101	30-34	-0.662284333415613
1101	35-39	-0.53517112410686
1101	40-44	-0.5986441450502369
1101	45-49	-0.5885435603426998
1101	50-54	-0.5188823824662876
1101	55-59	-0.7519591219757658
1101	60-64	-0.572836763183453
1101	65-69	-0.60280027237512
1101	70-74	-0.791277417214804
1101	75-79	-0.7509985399442165
1101	80-84	-0.6228010055015822
1101	85-89	-0.5904056152736388
1101	90-94	-0.6428249389329928
1101	95-99	-0.9026360917498835
1101	100-104	-0.7237076550466988
1101	105-109	-0.7461087398628194
1101	110-114	-0.7293691938707134
1101	115-119	-1.0099750998417427
1101	120-124	-1.1195154809728969
1101	125-129	-1.0415418328643575
1101	130-134	-0.9368140180548892
1101	135-139	-1.0951737307268274
1101	140-144	-1.2584779618768103
1101	145-149	-1.231855473550869
1101	150-154	-0.9362931859958756
1101	155-159	-1.263094798956292
1101	160-164	-1.2941711134991394
1101	165-169	-1.3087518293554865
1101	170-174	-1.6215171889773785
1101	175-179	-1.5738095314734437
1101	180-184	-1.4271860236621947
1101	185-189	-1.7763467999244895
1101	190-194	-1.8801816928167518
1101	195-199	-1.4867553089106273
1101	200-204	-2.209203635743904
1101	205-209	-2.1204102208546054
1101	210-214	-1.733623915838347
1101	215-219	-2.1809675068867094
1101	220-224	-2.3486590611027864
1101	225-229	-2.6190343220437597
1101	230-234	-2.56732301223634
1101	235-239	-3.22342410237896
1101	240-244	-2.84280077194893
1101	245-249	-2.883589231581933
1101	250-254	-3.110953964879677
1101	255-259	-3.1812486044769948
1101	260-264	-2.9966883988841175
1101	265-269	-3.5030573605179143
1101	270-274	-3.35120909350427
1101	275-279	-3.6281068619238397
1101	280-284	-3.6525903140734144
1101	285-289	-3.7467516258376534
1101	290-294	-4.143843768405404
1101	295-299	-4.25664656225559
1101	300-301	-2.391730193431183
1102	1	-0.4019537504986843
1102	2	-0.12805442246773424
1102	3	-0.6188782450371448
1102	4	-0.246726680745617
1102	5	-0.20543130429846457
1102	6	-0.4599560963166951
1102	7	-0.37340950537238626
1102	8	-0.16611164044753224
1102	9	-0.23394650525689542
1102	10-14	-0.40774463308102327
1102	15-19	-0.4563910874117951
1102	20-24	-0.38217482864462937
1102	25-29	-0.34801095983274877
1102	30-34	-0.501557590459683
1102	35-39	-0.40208775601415425
1102	40-44	-0.3748681284141071
1102	45-49	-0.6477185239209184
1102	50-54	-0.4731547629696067
1102	55-59	-0.5379105918439215
1102	60-64	-0.5343557176214659
1102	65-69	-0.743874065015806
1102	70-74	-0.6235419753475924
1102	75-79	-0.6841922312688808
1102	80-84	-0.8373866435325716
1102	85-89	-0.5873643211367892
1102	90-94	-0.7366831085950096
1102	95-99	-0.8493797814343935
1102	100-104	-1.150093793976822
1102	105-109	-1.1458696850394752
1102	110-114	-1.000247703362362
1102	115-119	-0.9817123219509014
1102	120-124	-1.123721330538995
1102	125-129	-1.2270229534490085
1102	130-134	-1.0923974528904523
1102	135-139	-1.1870580181811476
1102	140-144	-1.20160067807657
1102	145-149	-1.4488761502984424
1102	150-154	-1.333178927318869
1102	155-159	-1.614709927623224
1102	160-164	-1.6480821105038501
1102	165-169	-1.4526371524192427
1102	170-174	-1.9105792594130016
1102	175-179	-1.8689833634893773
1102	180-184	-1.645777373832491
1102	185-189	-1.960629005658248
1102	190-194	-2.0222765387335038
1102	195-199	-1.8524666532862355
1102	200-204	-2.051225824557225
1102	205-209	-2.1066224556225706
1102	210-214	-1.5364308431573583
1102	215-219	-2.2899289721549465
1102	220-224	-2.24810730854243
1102	225-229	-2.3928275999045496
1102	230-234	-2.370277951165008
1102	235-239	-2.9323728022760633
1102	240-244	-2.9171513394142465
1102	245-249	-3.4631575182919043
1102	250-254	-3.6349593605550155
1102	255-259	-3.7712576146380243
1102	260-264	-3.679830098984887
1102	265-269	-4.435365910615602
1102	270-274	-4.272616834471794
1102	275-279	-4.114691673703561
1102	280-284	-4.405251734474621
1102	285-289	-4.612035832524221
1102	290-294	-4.478494844194135
1102	295-299	-4.664417903636544
1102	300-301	-3.3216698243878433
1103	1	-0.5081608252625358
1103	2	-0.13065562172800327
1103	3	-0.39154494906394177
1103	4	-0.2688812396158511
1103	5	-0.6445342875050883
1103	6	-0.4417632682168957
1103	7	-0.6774774011874278
1103	8	-0.02286355048433819
1103	9	-0.3725463720057576
1103	10-14	-0.2440434269714089
1103	15-19	-0.2797038104050955
1103	20-24	-0.36894249220419084
1103	25-29	-0.27634865348687043
1103	30-34	-0.3610570854658519
1103	35-39	-0.5200823694133234
1103	40-44	-0.4162738816030398
1103	45-49	-0.5228541241154332
1103	50-54	-0.31704059695756115
1103	55-59	-0.4206192291087234
1103	60-64	-0.4846512325281367
1103	65-69	-0.5456826910585235
1103	70-74	-0.646779172818885
1103	75-79	-0.41730860429098726
1103	80-84	-0.4807861871677801
1103	85-89	-0.3634612799517356
1103	90-94	-0.45893024218771217
1103	95-99	-0.5576029750425846
1103	100-104	-0.6780567720051955
1103	105-109	-0.6322617950290237
1103	110-114	-0.6736092736851305
1103	115-119	-0.7296214436190951
1103	120-124	-0.9322034899204148
1103	125-129	-0.9445072301785693
1103	130-134	-0.7470766981734691
1103	135-139	-0.8621334898792696
1103	140-144	-0.8881965900262543
1103	145-149	-0.9205864777543624
1103	150-154	-1.104227858280808
1103	155-159	-1.174906074446099
1103	160-164	-1.1969549230522425
1103	165-169	-1.1180693331232092
1103	170-174	-1.3214867298545556
1103	175-179	-1.1671240493781205
1103	180-184	-1.1256147352056622
1103	185-189	-1.3422658748715683
1103	190-194	-1.728599869183519
1103	195-199	-1.4278941601063835
1103	200-204	-1.7424891614780975
1103	205-209	-1.9955133550942534
1103	210-214	-1.5605385888599166
1103	215-219	-2.227411537707418
1103	220-224	-2.1073003597377884
1103	225-229	-2.359683847419351
1103	230-234	-2.687522391479
1103	235-239	-3.093581023513032
1103	240-244	-2.9010660780713735
1103	245-249	-3.0655857777734496
1103	250-254	-3.2354034238584326
1103	255-259	-3.2253274689039273
1103	260-264	-3.5420386198092686
1103	265-269	-3.0691173998200156
1103	270-274	-3.655686583538543
1103	275-279	-3.7777239805247866
1103	280-284	-3.9713551323609053
1103	285-289	-4.243288363625911
1103	290-294	-4.612652321764678
1103	295-299	-4.347395585670515
1103	300-301	-2.6451054285363007
1104	1	-0.31089348406528217
1104	2	-0.9409542092481153
1104	3	-0.9130393078549233
1104	4	-0.08542695228170771
1104	5	-0.48195163305760147
1104	6	-0.2543495138444456
1104	7	-0.2783536491225007
1104	8	-0.5291954886060104
1104	9	-0.13110904421048275
1104	10-14	-0.40211800432006584
1104	15-19	-0.5175869396524249
1104	20-24	-0.48317349830001177
1104	25-29	-0.4387131266543136
1104	30-34	-0.748442214956242
1104	35-39	-0.5460447111651305
1104	40-44	-0.44519233157413396
1104	45-49	-0.7119486553152292
1104	50-54	-0.5244951307278782
1104	55-59	-0.47547764654596847
1104	60-64	-0.5652641339749067
1104	65-69	-0.9841405802518537
1104	70-74	-0.9432354297402519
1104	75-79	-0.6572747988471406
1104	80-84	-0.7590865893359933
1104	85-89	-0.5922949921391805
1104	90-94	-0.8224710805001934
1104	95-99	-0.9698736932612846
1104	100-104	-1.2185771653831274
1104	105-109	-1.2197316596897565
1104	110-114	-0.8689368317207098
1104	115-119	-1.0915766182653321
1104	120-124	-1.5848916868567855
1104	125-129	-1.4952438123447145
1104	130-134	-1.579256348595976
1104	135-139	-1.2834326084049508
1104	140-144	-1.371251662086408
1104	145-149	-1.6090316468780408
1104	150-154	-1.6559327390416527
1104	155-159	-1.660838401022854
1104	160-164	-2.0920757152052545
1104	165-169	-1.816127775528738
1104	170-174	-2.2599649744339985
1104	175-179	-1.8549433312262273
1104	180-184	-1.8519812311947668
1104	185-189	-2.249621832979326
1104	190-194	-2.0958501150911637
1104	195-199	-2.138073411195762
1104	200-204	-2.540480764543446
1104	205-209	-2.8465269161970568
1104	210-214	-2.062097083301744
1104	215-219	-2.6356820954455706
1104	220-224	-3.0526800657600255
1104	225-229	-3.1917609312978854
1104	230-234	-3.1412012196055805
1104	235-239	-3.5717068164809014
1104	240-244	-3.4082548917991105
1104	245-249	-4.179218065937757
1104	250-254	-4.120834394404312
1104	255-259	-4.324802110897924
1104	260-264	-4.634188766322431
1104	265-269	-4.553912353981829
1104	270-274	-4.963803479587153
1104	275-279	-4.756576940760386
1104	280-284	-4.890840817147655
1104	285-289	-5.1894761231889355
1104	290-294	-5.339860949191671
1104	295-299	-5.369830772163109
1104	300-301	-3.413026981200016
1201	1	-0.7432894084265982
1201	2	-0.36899588327248267
1201	3	-0.7074311541627161
1201	4	-0.2656370731593256
1201	5	-0.5678005458533626
1201	6	-0.4828684197428288
1201	7	-0.702271991458641
1201	8	-0.6450426959241682
1201	9	-0.6743157345123478
1201	10-14	-0.4015607810187234
1201	15-19	-0.5935643066063108
1201	20-24	-0.617987729529581
1201	25-29	-0.5977369897572871
1201	30-34	-0.824919151012903
1201	35-39	-0.7660353234919626
1201	40-44	-0.6065926947050215
1201	45-49	-0.6445889308479664
1201	50-54	-0.8433433749160528
1201	55-59	-0.7566287384957775
1201	60-64	-0.9641941492258219
1201	65-69	-0.9397954481989004
1201	70-74	-1.2593148436792028
1201	75-79	-1.048992718142177
1201	80-84	-1.037242383420491
1201	85-89	-1.1579186128751218
1201	90-94	-1.032037193190618
1201	95-99	-1.1903543552959803
1201	100-104	-1.3537344167344187
1201	105-109	-1.353964484436407
1201	110-114	-1.258916229779107
1201	115-119	-1.3852416365767084
1201	120-124	-1.1918529741347186
1201	125-129	-1.4291569484862272
1201	130-134	-1.3187944752229654
1201	135-139	-1.4612738990744525
1201	140-144	-1.5755802066125462
1201	145-149	-1.4415089046408731
1201	150-154	-1.727994683964262
1201	155-159	-1.8283303693244228
1201	160-164	-1.7026925496451781
1201	165-169	-1.6020498145092539
1201	170-174	-1.8648780498674
1201	175-179	-1.7083426312239318
1201	180-184	-1.7922675122219438
1201	185-189	-1.801088850923584
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2102	220-224	2.1787549439705955
2102	225-229	2.5155666022140935
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2102	235-239	2.8062475828178783
2102	240-244	2.748517470394617
2102	245-249	2.8561714147546517
2102	250-254	2.8218068170634183
2102	255-259	3.012520211177602
2102	260-264	2.846763471235075
2102	265-269	3.284228457961568
2102	270-274	3.261947395635225
2102	275-279	3.206648094090042
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2102	295-299	3.7399604804482145
2102	300-301	2.1865038997990602
2103	1	1.0739162576122396
2103	2	0.7571935992976364
2103	3	0.5508220340551944
2103	4	0.3636616855103938
2103	5	0.6749070552516656
2103	6	0.6885424251964025
2103	7	0.9401319018405871
2103	8	0.6481067413278012
2103	9	0.5456344091696863
2103	10-14	0.5401197037713033
2103	15-19	0.6504968094326031
2103	20-24	0.6492284885307171
2103	25-29	0.5810542404814782
2103	30-34	0.8477572728325597
2103	35-39	0.721015877489279
2103	40-44	0.7213001305481512
2103	45-49	0.7879041339977135
2103	50-54	0.7825129140693647
2103	55-59	0.7961856365257916
2103	60-64	0.873628142000733
2103	65-69	1.023836866189356
2103	70-74	1.1466111152405958
2103	75-79	0.8924361947245529
2103	80-84	0.9385230112782068
2103	85-89	0.807824260377231
2103	90-94	0.8763072097362112
2103	95-99	1.0710830035467964
2103	100-104	1.1226085081229513
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2103	110-114	1.0232555129731011
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2103	125-129	1.312617149378763
2103	130-134	1.3404957260027253
2103	135-139	1.3966858685627344
2103	140-144	1.2604826552567623
2103	145-149	1.2679462435239515
2103	150-154	1.3306264701090953
2103	155-159	1.4730869963668134
2103	160-164	1.63592946482623
2103	165-169	1.5417108309412164
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2103	235-239	2.3507077005104122
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2103	245-249	3.035539998578372
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2103	255-259	3.1150068995163487
2103	260-264	3.357570706377196
2103	265-269	3.359706821188329
2103	270-274	3.550429885639126
2103	275-279	3.5606272677014275
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2103	285-289	3.801804943351044
2103	290-294	3.7710882949183606
2103	295-299	4.006009178026609
2103	300-301	2.5942654752732786
2104	1	1.2218747812611213
2104	2	0.5871004234576773
2104	3	0.3977055028898846
2104	4	0.21424366134239392
2104	5	0.6226569366672479
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2104	7	0.7665110469049878
2104	8	0.814161412890428
2104	9	0.38175406167588477
2104	10-14	0.37337110605276536
2104	15-19	0.469498842782194
2104	20-24	0.6000419942178041
2104	25-29	0.514741170986035
2104	30-34	0.7240199482035976
2104	35-39	0.5901622343441346
2104	40-44	0.624345107730754
2104	45-49	0.5902775422598054
2104	50-54	0.5329118034232394
2104	55-59	0.7072994046850596
2104	60-64	0.7686428554048703
2104	65-69	0.8868540772187572
2104	70-74	0.865454900737106
2104	75-79	0.6941871894569545
2104	80-84	0.8350110578828307
2104	85-89	0.6301720281246546
2104	90-94	0.8420087036615342
2104	95-99	0.9487146436761904
2104	100-104	0.9696741305696932
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2104	110-114	0.8923925701594158
2104	115-119	1.0326290855123474
2104	120-124	1.1204424074029724
2104	125-129	1.2643465555949156
2104	130-134	1.2057885936211754
2104	135-139	1.3174355218705287
2104	140-144	1.1222397611999178
2104	145-149	1.2627543406576365
2104	150-154	1.3066046522848325
2104	155-159	1.5777774516266767
2104	160-164	1.5339609661398974
2104	165-169	1.3452430591056874
2104	170-174	1.769565474997414
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2104	180-184	1.5734747720763167
2104	185-189	1.8035502248013238
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2104	265-269	3.1885590256038583
2104	270-274	3.3235518146533316
2104	275-279	3.3999984679909936
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2104	295-299	3.939328734755982
2104	300-301	2.6178858818061705
2201	1	1.3499627371022243
2201	2	0.204867543577528
2201	3	0.414350552608866
2201	4	0.2772294142657046
2201	5	0.36218779863046535
2201	6	0.6867858747617603
2201	7	0.8111927750542733
2201	8	0.7360990957547244
2201	9	0.45672465257263184
2201	10-14	0.4234641091997915
2201	15-19	0.5813037070981437
2201	20-24	0.5439203762646727
2201	25-29	0.44144061497802767
2201	30-34	0.6804874373197123
2201	35-39	0.6220648273724265
2201	40-44	0.5676353077126564
2201	45-49	0.6500862920115438
2201	50-54	0.6576005513677998
2201	55-59	-0.0693046870567926
2201	60-64	-0.8816526765355093
2201	65-69	0.2541956107598011
2201	70-74	0.20973553611333529
2201	75-79	0.023885193668149896
2201	80-84	-0.013260430279061097
2201	85-89	-0.07993303437284993
2201	90-94	-0.02676356186861284
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2202	1	0.8719235517318857
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2202	4	0.04721421082302868
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2202	9	0.5076896846544727
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2202	15-19	0.38262443102252064
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2204	1	0.9076799874264765
2204	2	0.5407949106333021
2204	3	0.6542986719344199
2204	4	0.2876055491554581
2204	5	0.6514225586823486
2204	6	0.2198598047228515
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2204	8	0.5869421567145139
2204	9	0.3114587641160824
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2301	1	-2.6112150356957358
2301	2	0.35934496801441185
2301	3	0.39174273383811453
2301	4	0.24069224556523494
2301	5	0.44935106656970447
2301	6	0.48544777438216613
2301	7	0.401980560007523
2301	8	0.4368226410246834
2301	9	0.4389195310536991
2301	10-14	0.3953680756267417
2301	15-19	0.37919147100032546
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2301	25-29	0.4049037858107667
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2301	40-44	0.5258143232021624
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2301	60-64	0.46108004705056516
2301	65-69	0.7098178525995991
2301	70-74	0.694253965582682
2301	75-79	0.5731954335814748
2301	80-84	0.555123030320388
2301	85-89	0.5533724710925441
2301	90-94	0.5900522621702109
2301	95-99	0.6376485953392361
2301	100-104	0.6717019554066965
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2301	125-129	1.0111227419152335
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2301	135-139	1.1030106487080218
2301	140-144	1.0451864572019538
2301	145-149	0.9913652290119046
2301	150-154	1.1763522173812433
2301	155-159	1.1841407577494074
2301	160-164	1.076037206355938
2301	165-169	1.2333417204924793
2301	170-174	1.4606718125640228
2301	175-179	1.3092859171392526
2301	180-184	1.3419984317291593
2301	185-189	1.6828131541178557
2301	190-194	1.5891904576699787
2301	195-199	1.5434827693419493
2301	200-204	1.9732431054088444
2301	205-209	2.0143102290491193
2301	210-214	-3.3820646816735547
2301	215-219	2.0230377562195088
2301	220-224	2.1236045599221924
2301	225-229	2.3879780411344456
2301	230-234	2.346437080462131
2301	235-239	2.7735486557291367
2301	240-244	2.7948966845437155
2301	245-249	2.7953033530716134
2301	250-254	2.824295909874966
2301	255-259	2.9206360288719715
2301	260-264	2.930417448000046
2301	265-269	3.061706201985487
2301	270-274	3.0377142794445113
2301	275-279	3.059340214049268
2301	280-284	3.3698591462371894
2301	285-289	3.6626161847997345
2301	290-294	3.9683072605925958
2301	295-299	4.188510280448426
2301	300-301	2.501378467340178
2302	1	1.024823008277714
2302	2	0.7192102581452673
2302	3	0.5094859580438325
2302	4	0.28661064867155517
2302	5	0.5685801644621478
2302	6	0.5019062785559285
2302	7	0.7331061093252131
2302	8	0.7623884294839343
2302	9	0.5172573149992559
2302	10-14	0.5511426573743279
2302	15-19	0.6416728099687674
2302	20-24	0.6506070451190809
2302	25-29	0.5369198080147655
2302	30-34	0.7415941208632262
2302	35-39	0.743003054991064
2302	40-44	0.7120167244301143
2302	45-49	0.881728617880114
2302	50-54	0.7508097518059174
2302	55-59	0.8986166114968057
2302	60-64	0.7955100139615752
2302	65-69	1.1450170656439838
2302	70-74	1.2314378173777882
2302	75-79	0.9190342636026116
2302	80-84	1.0163433659375016
2302	85-89	0.9573917910635998
2302	90-94	1.042826848322811
2302	95-99	1.2259021934680234
2302	100-104	1.2343367391748572
2302	105-109	1.2771119191795997
2302	110-114	1.0251678333264351
2302	115-119	1.2754839491163992
2302	120-124	1.5394102030431647
2302	125-129	1.458077717691932
2302	130-134	1.5052267081833683
2302	135-139	1.6769218475906555
2302	140-144	1.5370822078294744
2302	145-149	1.5986876080908203
2302	150-154	1.8480610454659043
2302	155-159	1.8106324886521499
2302	160-164	1.9083808590004487
2302	165-169	1.7667859018607537
2302	170-174	2.0129025995969414
2302	175-179	1.808881096317858
2302	180-184	1.81052209968351
2302	185-189	1.997303745683194
2302	190-194	2.0451073572480674
2302	195-199	1.9559998148976447
2302	200-204	2.346833843129101
2302	205-209	2.4544756523670337
2302	210-214	2.8846997086509134
2302	215-219	2.3947581950216943
2302	220-224	2.402384467022628
2302	225-229	2.7894417932345625
2302	230-234	2.7588884968659997
2302	235-239	2.797872887780386
2302	240-244	3.142971040131613
2302	245-249	3.3928121354045224
2302	250-254	3.583262694238371
2302	255-259	3.5445577950702187
2302	260-264	3.8171759991992857
2302	265-269	3.8952391382720783
2302	270-274	3.959156149979261
2302	275-279	3.9563832248019537
2302	280-284	4.400235490323212
2302	285-289	4.345912614559939
2302	290-294	4.5487153650393175
2302	295-299	4.751191038208795
2302	300-301	3.053737490558337
2303	1	0.7282812560440348
2303	2	-0.05176862847375219
2303	3	0.09545064456025898
2303	4	0.0874303046876932
2303	5	0.45107587940916005
2303	6	0.4584641522402535
2303	7	0.7448111403974309
2303	8	0.3999864405759155
2303	9	0.5001599982735172
2303	10-14	0.2840709884109742
2303	15-19	0.2633745702810444
2303	20-24	0.3610815197867723
2303	25-29	0.2588140605924991
2303	30-34	0.45304124447114447
2303	35-39	0.40695016519423177
2303	40-44	0.3836324262225972
2303	45-49	0.4499446953631647
2303	50-54	0.4097670764315282
2303	55-59	0.5028811105254007
2303	60-64	0.6013652104484635
2303	65-69	0.6930238884744213
2303	70-74	0.8028753372881638
2303	75-79	0.5775172453799229
2303	80-84	0.5642141641778764
2303	85-89	0.6200465550563408
2303	90-94	0.614937294064589
2303	95-99	0.6328719869728587
2303	100-104	0.6588017529292216
2303	105-109	0.8696026674703923
2303	110-114	0.6236728646433605
2303	115-119	0.7926112150011093
2303	120-124	0.843663032318883
2303	125-129	0.8874229744548607
2303	130-134	0.6342327206742944
2303	135-139	0.9730125286442117
2303	140-144	1.0616205401295922
2303	145-149	1.1094652151634037
2303	150-154	1.2131455445360118
2303	155-159	1.3445965746595299
2303	160-164	1.2544060132908932
2303	165-169	1.3330032219751864
2303	170-174	1.3318598343623904
2303	175-179	1.51483909588487
2303	180-184	1.2993313657584196
2303	185-189	1.3423643648648316
2303	190-194	1.7878435194765423
2303	195-199	1.502331632751435
2303	200-204	1.6175518917687768
2303	205-209	1.9929912040737605
2303	210-214	2.3896560484069944
2303	215-219	1.8797549475655373
2303	220-224	1.906046154256515
2303	225-229	2.196225007494508
2303	230-234	2.2045210291601514
2303	235-239	2.181320603736218
2303	240-244	2.5912314861155927
2303	245-249	2.5509701061052574
2303	250-254	2.5145450785928496
2303	255-259	2.332522361715238
2303	260-264	2.796909707794221
2303	265-269	2.9440047390935433
2303	270-274	3.0202439923553825
2303	275-279	3.1841199433481684
2303	280-284	3.2257796459038914
2303	285-289	3.35942225963381
2303	290-294	3.663620870759992
2303	295-299	3.6766152052167094
2303	300-301	1.8670743085391166
2304	1	1.368034622851951
2304	2	0.3965846106276629
2304	3	0.3853262758367464
2304	4	0.12971313106489646
2304	5	0.6990949773368342
2304	6	0.5208734515959108
2304	7	0.8666402458713947
2304	8	0.7615935834330614
2304	9	0.5341491577325073
2304	10-14	0.45100915233969374
2304	15-19	0.3953407425687345
2304	20-24	0.5396021559973789
2304	25-29	0.48305652881099803
2304	30-34	0.6562593033322344
2304	35-39	0.6452719130319267
2304	40-44	0.625031828323948
2304	45-49	0.6582137435350575
2304	50-54	0.6338652390610662
2304	55-59	0.7212497290008386
2304	60-64	0.9227028670064144
2304	65-69	1.0585779605705525
2304	70-74	1.0203386216673351
2304	75-79	0.7779724555013559
2304	80-84	0.934480551106482
2304	85-89	0.8600618134961664
2304	90-94	0.9815035093357452
2304	95-99	1.0607211827259206
2304	100-104	1.049288115698623
2304	105-109	1.1886235842483082
2304	110-114	0.9458471923183538
2304	115-119	1.0462039418114557
2304	120-124	1.2958545257386795
2304	125-129	1.313657318659839
2304	130-134	1.3079971049326709
2304	135-139	1.3784718896948078
2304	140-144	1.3930893764193435
2304	145-149	1.53570423117295
2304	150-154	1.5090288050986942
2304	155-159	1.5379898026254146
2304	160-164	1.7077236163473408
2304	165-169	1.5853426745323382
2304	170-174	1.7855035907642147
2304	175-179	1.9123692004818587
2304	180-184	1.6405153334124591
2304	185-189	2.0801049818992006
2304	190-194	2.0044435311960918
2304	195-199	1.8848548521121344
2304	200-204	2.390098843220539
2304	205-209	2.374748896924377
2304	210-214	2.976691126848735
2304	215-219	2.6074475264479666
2304	220-224	2.509783154080246
2304	225-229	2.6953979624979674
2304	230-234	2.7943309816482724
2304	235-239	2.815371395846011
2304	240-244	3.018594576888283
2304	245-249	3.4346362432277644
2304	250-254	3.423532619595747
2304	255-259	3.367866859604412
2304	260-264	3.4835320986860587
2304	265-269	3.846558926371493
2304	270-274	3.8447907307523685
2304	275-279	3.998071588069248
2304	280-284	4.07068619123374
2304	285-289	4.058916730524405
2304	290-294	4.191875695934332
2304	295-299	4.636926151180141
2304	300-301	2.7802585662142327
2401	1	0.30941048097552226
2401	2	0.3876172193233103
2401	3	0.3598400058138651
2401	4	0.12120340566443843
2401	5	-0.08414300893318938
2401	6	0.477566815440305
2401	7	0.39558761429244527
2401	8	0.44942253080148475
2401	9	0.34062799068444605
2401	10-14	0.38543420954793106
2401	15-19	0.3563504679691931
2401	20-24	0.474876755539718
2401	25-29	0.3615216144499911
2401	30-34	0.5290030450977241
2401	35-39	0.5205765902387753
2401	40-44	0.49988725710991133
2401	45-49	0.46941480678702163
2401	50-54	0.44352043623226933
2401	55-59	0.6067532919035656
2401	60-64	0.708142166325409
2401	65-69	0.7811293853676773
2401	70-74	0.8327032187611252
2401	75-79	0.7083082780734458
2401	80-84	0.7169631399147889
2401	85-89	0.6875602633384617
2401	90-94	0.817823749452927
2401	95-99	1.0150805856680805
2401	100-104	1.0121080547706853
2401	105-109	1.0048882882805223
2401	110-114	0.9306378536291078
2401	115-119	0.9673528458175085
2401	120-124	1.142498516668386
2401	125-129	1.197421490995442
2401	130-134	1.1393803248873233
2401	135-139	1.3136456855225518
2401	140-144	1.3951387399128485
2401	145-149	1.5563989819766846
2401	150-154	1.5489203899494086
2401	155-159	1.504177107096094
2401	160-164	1.5315057480313818
2401	165-169	1.489774432097608
2401	170-174	1.7768058700773892
2401	175-179	1.8454415877776817
2401	180-184	1.673465176989005
2401	185-189	2.016647518986698
2401	190-194	1.9581328179916433
2401	195-199	1.648354844903686
2401	200-204	2.194774967378958
2401	205-209	2.339116822780017
2401	210-214	2.1818001206386555
2401	215-219	2.4170418520731225
2401	220-224	2.624973068956365
2401	225-229	2.689414733971219
2401	230-234	2.7037751862007298
2401	235-239	3.0204645243317536
2401	240-244	3.3281806604118174
2401	245-249	3.425138697987972
2401	250-254	3.5791952597057666
2401	255-259	3.6166920944667282
2401	260-264	3.7714241255233176
2401	265-269	4.041854060031646
2401	270-274	4.0593175407978315
2401	275-279	4.093116347074567
2401	280-284	4.060754300397086
2401	285-289	4.267777934197003
2401	290-294	4.685600931789811
2401	295-299	4.90572884566096
2401	300-301	4.643799698732
2402	1	0.7440451190899644
2402	2	0.3951469419124791
2402	3	0.5285582843371159
2402	4	0.28406059818338036
2402	5	0.6451276578261798
2402	6	0.5300558650127201
2402	7	0.7276045787643923
2402	8	0.5930417340275156
2402	9	0.44456319196373784
2402	10-14	0.4473336741936933
2402	15-19	0.44784598350837257
2402	20-24	0.5168248887525309
2402	25-29	0.4760135674647188
2402	30-34	0.632174672926169
2402	35-39	0.4741548749525535
2402	40-44	0.5183586044999302
2402	45-49	0.6390887118383901
2402	50-54	0.702020945788199
2402	55-59	0.5710683231051377
2402	60-64	0.6988631088809427
2402	65-69	0.858231066846173
2402	70-74	0.9604710719984553
2402	75-79	0.7733520567068126
2402	80-84	0.7362557545565593
2402	85-89	0.7849900871929165
2402	90-94	0.7926437320030573
2402	95-99	0.8028089143703028
2402	100-104	0.9806625540681395
2402	105-109	1.161853342995311
2402	110-114	0.9451023214257006
2402	115-119	1.035681196904477
2402	120-124	1.1106198589276595
2402	125-129	1.11929004389847
2402	130-134	1.1438306638204132
2402	135-139	1.1969446343387418
2402	140-144	1.2133562184751518
2402	145-149	1.4319487833885631
2402	150-154	1.3845750290523213
2402	155-159	1.4172972564231436
2402	160-164	1.4044191692206631
2402	165-169	1.435994240386382
2402	170-174	1.7482049690626909
2402	175-179	1.688480016075065
2402	180-184	1.5345689102042925
2402	185-189	1.7210742564811703
2402	190-194	1.8254982767913681
2402	195-199	1.845286694225969
2402	200-204	2.086921724266748
2402	205-209	2.2746023937237574
2402	210-214	2.8561470735537213
2402	215-219	2.445089309044352
2402	220-224	2.2876509179972473
2402	225-229	2.5177635843698027
2402	230-234	2.530728029117313
2402	235-239	2.821669959003323
2402	240-244	3.024184619817344
2402	245-249	3.2536936206492264
2402	250-254	3.4377211913461387
2402	255-259	3.5630650502609846
2402	260-264	3.7554832226776043
2402	265-269	3.8549796185265954
2402	270-274	4.054925872041405
2402	275-279	4.2558076481514675
2402	280-284	4.298986259906421
2402	285-289	4.406150969960741
2402	290-294	4.620741461901943
2402	295-299	4.612973639764615
2402	300-301	2.4516524386549463
2404	1	0.5883746968930375
2404	2	0.5887695880813126
2404	3	0.5249640033878222
2404	4	0.36834775501412054
2404	5	0.5452996758838466
2404	6	0.49403355848229324
2404	7	0.745961628713907
2404	8	0.5326003055841468
2404	9	0.495874301082722
2404	10-14	0.4671176113944142
2404	15-19	0.5094429944197572
2404	20-24	0.5825807787218693
2404	25-29	0.3585325072110379
2404	30-34	0.6556225778409441
2404	35-39	0.5507348785722073
2404	40-44	0.5449336457349503
2404	45-49	0.6463419429753756
2404	50-54	0.5867380805053344
2404	55-59	0.735415087451905
2404	60-64	0.7108452008630337
2404	65-69	0.9874424294350206
2404	70-74	1.011620672949391
2404	75-79	0.7513110216970631
2404	80-84	0.8801372753346399
2404	85-89	0.7163517140717843
2404	90-94	0.7971706992886425
2404	95-99	0.9375657667133623
2404	100-104	1.0648977441653997
2404	105-109	1.1769019725267107
2404	110-114	1.0134400756255246
2404	115-119	1.113974742439403
2404	120-124	1.2799832541531195
2404	125-129	1.4027206362945854
2404	130-134	1.352115367622364
2404	135-139	1.4294291613060253
2404	140-144	1.4286877834618927
2404	145-149	1.452841798419513
2404	150-154	1.5632752943043116
2404	155-159	1.7037782466972367
2404	160-164	1.7008382673639026
2404	165-169	1.556188758511933
2404	170-174	1.8608840141555376
2404	175-179	1.7556165979526916
2404	180-184	1.5518127553365844
2404	185-189	1.8768184591576365
2404	190-194	1.9447872746461066
2404	195-199	1.8143581009069365
2404	200-204	2.428246680850666
2404	205-209	2.495828259491457
2404	210-214	3.0692342121787064
2404	215-219	2.672815328045271
2404	220-224	2.6955250624450997
2404	225-229	2.7806791017873103
2404	230-234	2.8941819059177973
2404	235-239	3.081233755100982
2404	240-244	3.3066864075382547
2404	245-249	3.5114870622673706
2404	250-254	3.5686559139940073
2404	255-259	3.7909866537324746
2404	260-264	3.909692691457874
2404	265-269	4.159773911654177
2404	270-274	4.158013101065951
2404	275-279	4.327243163437
2404	280-284	4.158829348299275
2404	285-289	4.118770647579957
2404	290-294	4.953856487054409
2404	295-299	4.850771489226979
2404	300-301	3.497369397422201
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	14.0
16	701.0
17	1611.0
18	1599.0
19	1577.0
20	1578.0
21	1771.0
22	2182.0
23	2850.0
24	3711.0
25	5042.0
26	7346.0
27	11172.0
28	16733.0
29	22692.0
30	28594.0
31	32666.0
32	44824.0
33	56494.0
34	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	99.53905491866642	0.1393937400286189	0.044819816115396635	0.27673152518955907
2	0.3762397407852103	99.12375203539531	0.09087320515140052	0.40913501866807017
3	0.34046612608760013	0.4494317340745736	0.17434497277915756	99.03575716705866
4	0.1377489761344759	0.2615174591687363	99.37046661951678	0.23026694518001942
5	0.199838813138374	99.08345531998881	0.44737577920689486	0.26933008766591554
6	0.19531571242948076	0.5954045296797644	0.14227207684336915	99.06700768104739
7	0.33964374414052867	31.688843566506026	37.614516686129704	30.356996003223735
8	34.00014802875047	33.70038980904291	0.38281879636178223	31.916643365844834
9	99.32975871313673	0.16200924357308508	0.22533265349759043	0.28289938979259527
10-14	39.957400615141694	19.9689139624007	39.8919390121548	0.18174641030280103
15-19	0.1989435186274131	39.92415638161298	39.74799391079863	20.12890618896098
20-24	19.82897884114905	0.42409825655805933	39.82452070974768	39.92240219254521
25-29	20.051445813261733	0.23847363710547792	54.74833175392877	24.96174879570402
30-34	0.29402086281220174	28.980140840435688	0.2769094080094771	70.44892888874263
35-39	14.52679715223435	5.81499725606771	44.57067538581663	35.087530205881315
40-44	39.12211965828846	12.313432528759185	26.707055844539518	21.85739196841283
45-49	0.7384799757681113	20.02628962374725	20.467879578049068	58.767350822435574
50-54	36.99211019385346	2.9473167743996207	24.55784485796044	35.50272817378648
55-59	30.34333184298478	29.209761206638884	39.79650566785318	0.6504012825231519
60-64	24.69490468371669	17.045133427967187	17.18617796492176	41.073783923394366
65-69	28.985296870914823	39.09389399923956	7.432144321105056	24.488664808740555
70-74	43.65675090443371	14.874386041353086	3.3527588528875865	38.116104201325626
75-79	0.5989971048748362	0.24032509911759756	39.820515444518904	59.34016235148866
80-84	19.99275316863162	22.65666526902417	1.2817317120680376	56.06884985027617
85-89	58.86078613779823	0.9857470323170173	38.64579059933123	1.5076762305535354
90-94	0.3235992157672067	0.2427819626457538	66.40132081312558	33.03229800846146
95-99	5.185717930700207	34.81735376617286	15.97819095220126	44.01873735092568
100-104	0.173290747497118	19.629784192280837	59.342205841641835	20.854719218580207
105-109	20.098082570877594	39.44760980561202	0.6451495581490118	39.80915806536138
110-114	0.22055656639389187	20.17067507008127	49.123932454138206	30.484835909386632
115-119	20.035259105639224	0.6363011676684586	19.817783852751887	59.51065587394043
120-124	0.458156324029592	44.85539828747635	10.350246820449703	44.33619856804436
125-129	48.84486810724151	44.72052566835674	5.575588816559579	0.8590174078421737
130-134	8.699832288202835	11.568142535997637	39.52272879587563	40.2092963799239
135-139	2.5466773598975703	10.874155073366985	34.15891366043695	52.4202539062985
140-144	4.450252461071156	1.0889893195596687	43.57654425954273	50.88421395982644
145-149	16.461478579887537	26.7797368137738	17.921259451267485	38.83752515507118
150-154	0.672099025240997	36.44281317188154	21.660777238776603	41.22431056410086
155-159	21.77701604582105	36.82624085555612	25.358049472612045	16.03869362601079
160-164	21.91244967335865	33.057878642438595	21.286273105492597	23.743398578710163
165-169	11.694380144459405	21.290683097984335	46.99921734574083	20.01571941181543
170-174	28.513848054969255	36.8608310906354	14.363578309206188	20.261742545189154
175-179	16.166485408511086	15.860876692192127	34.342986885536924	33.62965101375986
180-184	47.89518269517175	5.155821279907472	34.86146960943665	12.087526415484142
185-189	31.99941906303166	27.85277397402382	16.029295821403377	24.118511141541145
190-194	38.94439657390851	16.953225782351215	11.9029119121263	32.19946573161398
195-199	10.263180181032894	9.362582217860517	64.20507098010498	16.16916662100161
200-204	1.2365719583528214	29.97347413998643	19.473952236836965	49.31600166482378
205-209	12.764683975313895	34.529953181527986	13.0974509204086	39.60791192274952
210-214	13.682100743725137	24.382433436091528	49.11994221123422	12.81552360894912
215-219	4.445297709427736	23.641594280274226	18.939178772247935	52.9739292380501
220-224	24.477213203684155	10.971210582146378	22.280487876012764	42.27108833815671
225-229	19.175843327179425	10.816785325348263	12.405877732360048	57.60149361511226
230-234	5.085977213994592	4.727870898210386	58.663286636870346	31.52286525092467
235-239	11.69673909142579	24.487235876921588	0.9957743645897928	62.82025066706283
240-244	14.484017273205648	45.76497414865982	20.794333586695956	18.95667499143857
245-249	4.862105246443885	24.775844245492024	30.85080156209129	39.511248945972795
250-254	34.477669227443656	43.6293181230929	8.470716914424	13.422295735039441
255-259	20.454172581898053	20.330203841105725	19.628495566924705	39.587128010071524
260-264	24.172393772416562	11.86694291940385	17.412049165837935	46.548614142341656
265-269	4.703032783516741	20.120653808445095	20.072177580667212	55.104135827370946
270-274	15.41424395420255	20.075590307918848	62.59950401206221	1.9106617258163903
275-279	24.743530543773282	15.841515039114903	1.273747132933479	58.14120728417833
280-284	54.89542124665557	0.46544031917833145	19.878387394612517	24.760751039553583
285-289	41.45711769053461	4.573319923998771	19.853296030184502	34.11626635528212
290-294	24.516931407178664	33.27887645677785	19.831299308361004	22.37289282768248
295-299	33.78429693923392	19.83700212753333	40.74285665850123	5.635844274731515
300-301	20.808142676995136	1.8519185732300487	0.35885426049360475	76.98108448928122
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.25
2	0.0
3	0.0
4	0.0
5	0.75
6	1.5
7	2.5
8	2.0
9	0.75
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	1.0
16	1.0
17	0.5
18	0.0
19	2.0
20	4.0
21	5.5
22	7.0
23	7.0
24	6.0
25	4.5
26	5.083333333333334
27	5.083333333333334
28	4.5
29	8.583333333333332
30	13.750000000000002
31	13.083333333333332
32	15.916666666666668
33	18.0
34	19.5
35	21.0
36	25.666666666666664
37	31.249999999999993
38	31.999999999999993
39	34.999999999999986
40	38.583333333333336
41	42.000000000000014
42	47.25000000000002
43	63.41666666666669
44	86.83333333333329
45	130.41666666666646
46	178.33333333333331
47	1055.2500000000005
48	1751.9166666666645
49	1327.9166666666665
50	1735.0833333333308
51	3255.4166666666706
52	16822.083333333376
53	25123.49999999998
54	14428.41666666667
55	13403.166666666664
56	2719.666666666666
57	574.2499999999999
58	149.0
59	62.91666666666667
60	30.666666666666668
61	13.083333333333334
62	6.0
63	5.5
64	2.25
65	0.5
66	0.5833333333333333
67	1.0833333333333333
68	0.75
69	0.0
70	0.5833333333333333
71	0.25
72	0.0
73	0.5833333333333333
74	0.3333333333333333
75	0.0
76	0.75
77	1.0
78	1.0
79	1.25
80	0.75
81	0.25
82	0.25
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.5833333333333333
89	1.1666666666666665
90	1.4166666666666665
91	0.5
92	1.3333333333333333
93	1.0833333333333333
94	1.5833333333333333
95	1.5833333333333333
96	5.166666666666666
97	13.000000000000004
98	21.666666666666668
99	34.75
100	95.83333333333317
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	8.226654195058908E-5
35-39	8.227676257394623E-5
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	8.293483146812897E-5
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	8.312932012685533E-5
210-214	1.8513525658308618
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
10-19	38.0
20-29	39.0
30-39	49.0
40-49	114.0
50-59	189.0
60-69	389.0
70-79	54.0
80-89	41.0
90-99	221.0
100-109	119.0
110-119	121.0
120-129	77.0
130-139	127.0
140-149	194.0
150-159	139.0
160-169	104.0
170-179	115.0
180-189	116.0
190-199	144.0
200-209	307.0
210-219	439.0
220-229	272.0
230-239	276.0
240-249	275.0
250-259	430.0
260-269	463.0
270-279	747.0
280-289	962.0
290-299	1083.0
300-302	235552.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.2599878287471835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.84911644766652	4.998437474300564
2	10.506343452650658	1.5255185118176287
3	5.488219302220208	1.1953321600684221
4	3.3019936565473493	0.9588973502853666
5	2.4071137290439513	0.8737808187634665
6	1.551880380607159	0.6759979604927713
7	1.2063887630267331	0.6130857415418017
8	0.8608971454463071	0.5000082238194707
9	0.696647032170367	0.4551884077040741
>10	4.162890801993656	5.3878353262389185
>50	0.19823289533303123	1.0193424233951216
>100	0.3341640235613956	4.71060379282554
>500	0.18124150430448574	7.978338459514137
>1k	0.16991391028545538	34.63584927383674
>5k	0.08495695514272769	34.47178407539598
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6186	2.543627362292143	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	6147	2.5275909143242488	No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6126	2.518955903879998	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	6103	2.5094985114886756	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	6067	2.4946956364413886	No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6054	2.4893501537854243	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	5407	2.2233095939077945	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	5401	2.22084244806658	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	5301	2.1797233507130054	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	5283	2.1723219131893616	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	5225	2.1484728367242885	No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	5213	2.143538545041859	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	5146	2.115988749814964	No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	5129	2.108998503264856	No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	5046	2.074869652461389	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	4948	2.034572937054886	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	4938	2.0304610273195283	No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	4926	2.0255267356370994	No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	4889	2.0103126696162765	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	4690	1.9284856658826626	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	4631	1.9042253984440534	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	4582	1.8840770407408016	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	4541	1.867218210825836	No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	4499	1.8499481899373345	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	4246	1.74591687363279	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	4132	1.6990411026497145	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	4076	1.6760144081317128	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	2302	0.946561621079294	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	2286	0.9399825655027221	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	2236	0.9194230168259346	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	1980	0.814158127600783	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	1976	0.81251336370664	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	1904	0.7829076136120661	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	1864	0.766459974670636	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	1807	0.7430220891790984	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	1616	0.6644846132337703	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	1473	0.6056843040181582	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	1429	0.5875919011825852	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	1358	0.558397342061547	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	1244	0.5115215710784717	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	1226	0.5041201335548282	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	1172	0.48191582098389774	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	1132	0.46546818204246776	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	1105	0.4543660257570026	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	1025	0.4214707478741426	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA	729	0.29975821970756095	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	726	0.29852464678695373	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	705	0.289889636342703	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA	704	0.2894784453691672	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG	693	0.28495534466027406	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA	682	0.2804322439513808	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG	665	0.27344199740127306	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG	664	0.2730308064277373	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	662	0.2722084244806658	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA	646	0.26562936890409383	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG	626	0.25740554943337884	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	621	0.2553495945657001	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA	615	0.25288244872448556	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG	610	0.25082649385680683	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA	605	0.2487705389891281	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG	595	0.2446586292537706	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	590	0.24260267438609184	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG	584	0.2401355285448774	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA	577	0.23725719173012713	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	577	0.23725719173012713	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG	570	0.23437885491537688	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG	566	0.23273409102123388	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	562	0.2310893271270909	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA	558	0.2294445632329479	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA	547	0.22492146252405468	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	542	0.22286550765637592	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	539	0.2216319347357687	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG	538	0.22122074376223294	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG	533	0.21916478889455418	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG	531	0.21834240694748266	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG	524	0.21546407013273244	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG	517	0.2125857333179822	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG	493	0.20271714995312423	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG	479	0.19696047632362373	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG	446	0.18339117419694403	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG	405	0.16653234428197833	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC	340	0.13980493100215463	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC	312	0.1282915837431537	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC	311	0.12788039276961793	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC	283	0.11636704551061694	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC	280	0.11513347259000971	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC	264	0.10855441701343772	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC	261	0.10732084409283046	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC	251	0.10320893435747298	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG	245	0.1007417885162585	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	2.467145841214493E-4	0.0
155-159	0.0	0.0	0.0	4.1119097353574895E-4	0.0
160-164	0.0	0.0	0.0	4.1119097353574895E-4	0.0
165-169	0.0	0.0	0.0	4.1119097353574895E-4	0.0
170-174	0.0	0.0	0.0	4.1119097353574895E-4	0.0
175-179	0.0	0.0	0.0	4.1119097353574895E-4	0.0
180-184	0.0	0.0	0.0	4.1119097353574895E-4	0.0
185-189	0.0	0.0	0.0	4.1119097353574895E-4	0.0
190-194	0.0	0.0	0.0	4.1119097353574895E-4	0.0
195-199	0.0	0.0	0.0	4.1119097353574895E-4	0.0
200-204	0.0	0.0	0.0	4.1119097353574895E-4	0.0
205-209	0.0	0.0	0.0	4.1119097353574895E-4	0.0
210-214	0.0	0.0	0.0	4.1119097353574895E-4	0.0
215-219	0.0	0.0	0.0	4.1119097353574895E-4	0.0
220-224	0.0	0.0	0.0	4.1119097353574895E-4	0.0
225-229	0.0	0.0	0.0	4.1119097353574895E-4	0.0
230-234	0.0	0.0	0.0	4.1119097353574895E-4	0.0
235-239	0.0	0.0	0.0	4.1119097353574895E-4	0.0
240-244	0.0	0.0	0.0	4.1119097353574895E-4	0.0
245-249	0.0	0.0	0.0	4.1119097353574895E-4	0.0
250-254	0.0	0.0	0.0	4.1119097353574895E-4	0.0
255-259	0.0	0.0	0.0	4.1119097353574895E-4	0.0
260-264	0.0	0.0	0.0	4.1119097353574895E-4	0.0
265-269	0.0	0.0	0.0	4.1119097353574895E-4	0.0
270-274	0.0	0.0	0.0	4.1119097353574895E-4	0.0
275-279	0.0	0.0	0.0	7.401437523643481E-4	0.0
280-284	0.0	0.0	0.0	8.223819470714979E-4	0.0
285-289	0.0	0.0	0.0	8.223819470714979E-4	0.0
290	0.0	0.0	0.0	8.223819470714979E-4	0.0
>>END_MODULE
