##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IPMS2_cleaned_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	243196
Sequences flagged as poor quality	0
Sequence length	15-301
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30625503708943	34.0	34.0	34.0	20.0	34.0
2	33.12877267718219	34.0	34.0	34.0	34.0	34.0
3	33.629031727495516	34.0	34.0	34.0	34.0	34.0
4	33.43240842777019	34.0	34.0	34.0	34.0	34.0
5	33.30244329676475	34.0	34.0	34.0	34.0	34.0
6	33.146889751476174	34.0	34.0	34.0	34.0	34.0
7	33.55529285021135	34.0	34.0	34.0	34.0	34.0
8	33.49639385516209	34.0	34.0	34.0	34.0	34.0
9	33.43199723679666	34.0	34.0	34.0	34.0	34.0
10-14	33.44703037878913	34.0	34.0	34.0	34.0	34.0
15-19	33.44442137343244	34.0	34.0	34.0	34.0	34.0
20-24	33.60362595655267	34.0	34.0	34.0	34.0	34.0
25-29	33.47085466654657	34.0	34.0	34.0	34.0	34.0
30-34	33.45906271971849	34.0	34.0	34.0	34.0	34.0
35-39	33.55891730345178	34.0	34.0	34.0	34.0	34.0
40-44	33.53473439648146	34.0	34.0	34.0	34.0	34.0
45-49	33.61889726763563	34.0	34.0	34.0	34.0	34.0
50-54	33.46301966213246	34.0	34.0	34.0	34.0	34.0
55-59	33.44214653923338	34.0	34.0	34.0	34.0	34.0
60-64	33.40961806622477	34.0	34.0	34.0	34.0	34.0
65-69	33.440693425776864	34.0	34.0	34.0	34.0	34.0
70-74	33.5232040868433	34.0	34.0	34.0	34.0	34.0
75-79	33.42859298963526	34.0	34.0	34.0	34.0	34.0
80-84	33.607253950781015	34.0	34.0	34.0	34.0	34.0
85-89	33.556662862734456	34.0	34.0	34.0	34.0	34.0
90-94	33.31426570053634	34.0	34.0	34.0	34.0	34.0
95-99	33.44770392002814	34.0	34.0	34.0	34.0	34.0
100-104	33.282956868582104	34.0	34.0	34.0	34.0	34.0
105-109	33.39518783244027	34.0	34.0	34.0	34.0	34.0
110-114	33.1107997787916	34.0	34.0	34.0	34.0	34.0
115-119	33.25816436738164	34.0	34.0	34.0	34.0	34.0
120-124	33.3120394224094	34.0	34.0	34.0	34.0	34.0
125-129	33.204255586987266	34.0	34.0	34.0	34.0	34.0
130-134	33.25587304131717	34.0	34.0	34.0	34.0	34.0
135-139	33.26823557962581	34.0	34.0	34.0	34.0	34.0
140-144	33.3413424128953	34.0	34.0	34.0	34.0	34.0
145-149	33.21144104990272	34.0	34.0	34.0	34.0	34.0
150-154	33.19413992454056	34.0	34.0	34.0	34.0	34.0
155-159	33.18999723553442	34.0	34.0	34.0	34.0	34.0
160-164	33.2751844182318	34.0	34.0	34.0	34.0	34.0
165-169	33.25021564216107	34.0	34.0	34.0	34.0	34.0
170-174	32.782155658672814	34.0	34.0	34.0	31.2	34.0
175-179	33.14921018884579	34.0	34.0	34.0	34.0	34.0
180-184	33.047668811905304	34.0	34.0	34.0	34.0	34.0
185-189	33.0199267768768	34.0	34.0	34.0	34.0	34.0
190-194	33.16675651700043	34.0	34.0	34.0	34.0	34.0
195-199	32.90172637691939	34.0	34.0	34.0	34.0	34.0
200-204	32.9773772142365	34.0	34.0	34.0	34.0	34.0
205-209	32.713467156400974	34.0	34.0	34.0	31.2	34.0
210-214	32.80466471687594	34.0	34.0	34.0	34.0	34.0
215-219	32.99537231636403	34.0	34.0	34.0	34.0	34.0
220-224	33.12981376054133	34.0	34.0	34.0	34.0	34.0
225-229	33.141740343869245	34.0	34.0	34.0	34.0	34.0
230-234	32.89556926956084	34.0	34.0	34.0	34.0	34.0
235-239	32.999521296663765	34.0	34.0	34.0	34.0	34.0
240-244	32.95901122636681	34.0	34.0	34.0	34.0	34.0
245-249	32.771092292687186	34.0	34.0	34.0	34.0	34.0
250-254	32.655069704315835	34.0	34.0	34.0	31.2	34.0
255-259	32.61678551869938	34.0	34.0	34.0	34.0	34.0
260-264	32.62029316750495	34.0	34.0	34.0	34.0	34.0
265-269	32.47077123688708	34.0	34.0	34.0	31.2	34.0
270-274	32.47029091379137	34.0	34.0	34.0	31.2	34.0
275-279	31.96404962557611	34.0	34.0	34.0	22.8	34.0
280-284	31.98514199469248	34.0	34.0	34.0	22.8	34.0
285-289	32.07815231070374	34.0	34.0	34.0	20.0	34.0
290-294	32.02101126730301	34.0	34.0	34.0	25.6	34.0
295-299	31.46012566228226	34.0	34.0	34.0	20.0	34.0
300-301	30.217069298104967	34.0	27.0	34.0	14.5	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.26193393117534214
1101	2	-0.1433765202613344
1101	3	0.0638731201215279
1101	4	-0.0795940316995356
1101	5	-0.24614496881720527
1101	6	-0.25406803134391964
1101	7	-0.04103104633995969
1101	8	0.06949507396151233
1101	9	-0.008878197746675198
1101	10-14	-0.08224561280542986
1101	15-19	-0.02325680542796249
1101	20-24	-0.031351773818897755
1101	25-29	-0.04090222331176818
1101	30-34	-0.08315002609353428
1101	35-39	0.01758673098155583
1101	40-44	-0.11320624605309604
1101	45-49	-0.025434207123105068
1101	50-54	-0.10503456996740823
1101	55-59	-0.15307515087472723
1101	60-64	-0.06820070562691427
1101	65-69	-0.057786721068232794
1101	70-74	-0.18699791807388522
1101	75-79	-0.08529409971254864
1101	80-84	-0.04137744808837596
1101	85-89	-0.045829145994837006
1101	90-94	-0.11830136265507463
1101	95-99	-0.03766185426788837
1101	100-104	-0.14823628519213372
1101	105-109	-0.2240465386400885
1101	110-114	-0.2373012805714083
1101	115-119	-0.16997604113129938
1101	120-124	-0.03287183430902019
1101	125-129	-0.15446602143533283
1101	130-134	-0.18676350124427188
1101	135-139	-0.14311381983598181
1101	140-144	-0.1715350217870082
1101	145-149	-0.2130862087903651
1101	150-154	-0.10689301082169322
1101	155-159	-0.27299371647124815
1101	160-164	-0.1185273130489648
1101	165-169	-0.2554024158100674
1101	170-174	-0.3995218167470256
1101	175-179	-0.07057932270576828
1101	180-184	-0.18767205991029812
1101	185-189	-0.17033296495451822
1101	190-194	-0.2044282082751181
1101	195-199	-0.29681488405942247
1101	200-204	0.02480376728023259
1101	205-209	-0.27188964769899826
1101	210-214	-0.20386213873175052
1101	215-219	-0.2914270092983742
1101	220-224	-0.21240831456988474
1101	225-229	-0.28399777621263667
1101	230-234	-0.39961124929018155
1101	235-239	-0.27095541168414883
1101	240-244	-0.35801741156090827
1101	245-249	-0.3296873317555722
1101	250-254	-0.3778376618166064
1101	255-259	-0.38077716144535145
1101	260-264	-0.46807515381791376
1101	265-269	-0.5186168593454781
1101	270-274	-0.5132054989369479
1101	275-279	-0.6097560221073479
1101	280-284	-0.6969391222801029
1101	285-289	-0.6244245485509694
1101	290-294	-0.6778500634805553
1101	295-299	-0.9422788160198081
1101	300-301	-0.7711695028500856
1102	1	0.34859207423887995
1102	2	0.20908202540643828
1102	3	-0.04439628618835201
1102	4	0.19849003158066125
1102	5	0.10193238439244823
1102	6	0.2028288330582555
1102	7	0.07828126210566211
1102	8	0.08184220827777722
1102	9	0.17171337658096064
1102	10-14	0.0679088397193155
1102	15-19	0.10153478945304784
1102	20-24	0.040984297948632786
1102	25-29	-0.02406508458257406
1102	30-34	0.06580870395005434
1102	35-39	0.032251559935204455
1102	40-44	0.014589465546862357
1102	45-49	0.039722391415189406
1102	50-54	0.05059251937325371
1102	55-59	0.002933989329470421
1102	60-64	0.06064685486951049
1102	65-69	-9.959483220001175E-4
1102	70-74	-0.08626766895495308
1102	75-79	-0.1615347071250639
1102	80-84	-0.041444851049270426
1102	85-89	-0.04185962543239441
1102	90-94	-0.16176690251349157
1102	95-99	-0.0841103529651619
1102	100-104	-0.1643145094695626
1102	105-109	-0.1548458347184365
1102	110-114	-0.17845028286989617
1102	115-119	-0.08268890880950153
1102	120-124	-0.06294913195191754
1102	125-129	-0.17463340370868963
1102	130-134	-0.14652812370998447
1102	135-139	-0.1841380010526379
1102	140-144	-0.10207303199264572
1102	145-149	-0.1875041784647209
1102	150-154	-0.19605365350724213
1102	155-159	-0.17262036229392663
1102	160-164	-0.12394654388253201
1102	165-169	-0.22821745669228477
1102	170-174	-0.23890529142316552
1102	175-179	-0.1628533707516766
1102	180-184	-0.3030506047376633
1102	185-189	-0.20601415180392735
1102	190-194	-0.22457412237505991
1102	195-199	-0.3651057354285072
1102	200-204	0.09197736842801163
1102	205-209	-0.17339610394003557
1102	210-214	-0.2360310083553685
1102	215-219	-0.14357237393099354
1102	220-224	-0.19897902796162015
1102	225-229	-0.13655310451935776
1102	230-234	-0.18595637582029667
1102	235-239	-0.20855485000643625
1102	240-244	-0.1738637438665407
1102	245-249	-0.3184632406660981
1102	250-254	-0.2446034819328844
1102	255-259	-0.45091404210855046
1102	260-264	-0.5588341024716001
1102	265-269	-0.4331146422643144
1102	270-274	-0.5184172462539536
1102	275-279	-0.7886069956951651
1102	280-284	-0.6973632595463499
1102	285-289	-0.6613373433585004
1102	290-294	-0.7821450562180843
1102	295-299	-1.0639724531260626
1102	300-301	-1.302032474864113
1103	1	-0.3573167421298393
1103	2	-0.1581291651987371
1103	3	-0.16220990893818765
1103	4	0.024675126132933656
1103	5	-0.10086464423164188
1103	6	-0.2570136939811434
1103	7	-0.2718952645227759
1103	8	-0.43421019553544227
1103	9	-0.18991403081260927
1103	10-14	-0.20159346104220788
1103	15-19	-0.08781259129030161
1103	20-24	-0.06690958682025894
1103	25-29	0.008942281590321954
1103	30-34	-0.016823192886150196
1103	35-39	-0.028683439660063925
1103	40-44	-0.0516460029180692
1103	45-49	-0.13654271608859148
1103	50-54	-0.053168400682288564
1103	55-59	-0.20337800514122506
1103	60-64	-0.021982239956734873
1103	65-69	-0.0013191134724692688
1103	70-74	-0.10487612853440709
1103	75-79	-0.029773301387791662
1103	80-84	0.04988790942869059
1103	85-89	-0.008203438283040043
1103	90-94	-0.07454344533132229
1103	95-99	-0.09064059287948822
1103	100-104	-0.0646980835822859
1103	105-109	-0.12286781736485608
1103	110-114	-0.17648923878770262
1103	115-119	-0.03246393228320699
1103	120-124	-0.049459701585412574
1103	125-129	-0.03764754754709543
1103	130-134	-0.0018178442707323939
1103	135-139	-0.03501644404290971
1103	140-144	-0.027411940360821063
1103	145-149	0.020923999211390765
1103	150-154	-0.062284121647877555
1103	155-159	-0.1549354420953719
1103	160-164	0.0028667086860068025
1103	165-169	-0.10201448268081492
1103	170-174	-0.2983776546260586
1103	175-179	-0.08992924878149466
1103	180-184	-0.0653266081538888
1103	185-189	-0.1359473229848902
1103	190-194	-0.16345873442944026
1103	195-199	-0.32841175474511175
1103	200-204	0.022143850115021735
1103	205-209	-0.141742385849021
1103	210-214	0.008909758798544942
1103	215-219	-0.12168778549103365
1103	220-224	-0.09435800010447792
1103	225-229	-0.13240704143736082
1103	230-234	-0.22186006045753714
1103	235-239	-0.17309070850881625
1103	240-244	-0.38445328221683894
1103	245-249	-0.3226362781216139
1103	250-254	-0.35512600991530974
1103	255-259	-0.30456454115213205
1103	260-264	-0.3470458442929143
1103	265-269	-0.17681658311897053
1103	270-274	-0.42327175326549593
1103	275-279	-0.43484459473690507
1103	280-284	-0.33936991553057183
1103	285-289	-0.6686127829031392
1103	290-294	-0.36787418154587925
1103	295-299	-0.5973563298744331
1103	300-301	-0.7572617684858685
1104	1	0.16307734021668097
1104	2	0.38149377617639857
1104	3	-0.09396984695594313
1104	4	-0.09743874942274289
1104	5	0.09229194191618006
1104	6	0.07247784076696462
1104	7	0.014023794863120997
1104	8	0.012217802832481084
1104	9	0.032300550600972144
1104	10-14	0.040554767589121354
1104	15-19	-0.08764021464733673
1104	20-24	-0.09281101121980839
1104	25-29	0.01535047498223463
1104	30-34	-0.08990722191511935
1104	35-39	-0.02335137775727958
1104	40-44	-0.00498755776150972
1104	45-49	-0.09543333180023694
1104	50-54	-0.10555531289398346
1104	55-59	-0.07090013674346096
1104	60-64	-0.03866701533987538
1104	65-69	-0.0974860254090899
1104	70-74	-0.11165902718213516
1104	75-79	-0.1342234611570987
1104	80-84	-0.14220752121492808
1104	85-89	-0.09176668354841411
1104	90-94	-0.15966021980980116
1104	95-99	-0.11221615646599759
1104	100-104	-0.06562956578343915
1104	105-109	-0.12937411649794228
1104	110-114	-0.24510771359270933
1104	115-119	-0.021577463561605725
1104	120-124	-0.09858437148145782
1104	125-129	-0.16981537542975644
1104	130-134	-0.11928510655848612
1104	135-139	-0.11102798879721121
1104	140-144	-0.0907703848030792
1104	145-149	-0.11537605324533473
1104	150-154	-0.1310904931542325
1104	155-159	-0.004663845271906553
1104	160-164	-0.1372643519468113
1104	165-169	-0.20610617290839883
1104	170-174	-0.37287461067985106
1104	175-179	-0.1648987260068111
1104	180-184	-0.2459996918258227
1104	185-189	-0.2156199090004307
1104	190-194	-0.12101237852858304
1104	195-199	-0.34872067076435087
1104	200-204	0.08217344685937888
1104	205-209	-0.2667784568812053
1104	210-214	-0.13492024490105337
1104	215-219	-0.22548726754801152
1104	220-224	-0.16079066751105842
1104	225-229	-0.09734784794864737
1104	230-234	-0.2427461129917532
1104	235-239	-0.22592552167936475
1104	240-244	-0.21126466168046676
1104	245-249	-0.48534371626059425
1104	250-254	-0.3634797166372934
1104	255-259	-0.2958978250954303
1104	260-264	-0.5452505111403312
1104	265-269	-0.5113985874213256
1104	270-274	-0.4605022381454482
1104	275-279	-0.7775296700280556
1104	280-284	-0.8632448753505635
1104	285-289	-0.7988742002065052
1104	290-294	-0.8822878356032646
1104	295-299	-1.0011539788043358
1104	300-301	-1.0737179149612075
1201	1	-0.5388972406206918
1201	2	0.14035735457026277
1201	3	-0.014565526513308669
1201	4	0.05248027812298517
1201	5	0.15389137805208009
1201	6	0.3318945674271987
1201	7	0.07060641016019531
1201	8	0.09831423627131386
1201	9	-0.3413394064753277
1201	10-14	-0.050592596240569776
1201	15-19	-0.04841755020835592
1201	20-24	-0.030700233657860565
1201	25-29	-0.04924788504257549
1201	30-34	0.007987796128233526
1201	35-39	0.017031615159702085
1201	40-44	-0.08118044878752784
1201	45-49	-0.08211219338638642
1201	50-54	-0.09414793164729218
1201	55-59	-0.06227826829464078
1201	60-64	-0.07430908237873268
1201	65-69	-0.03553462699291998
1201	70-74	-0.02091765757796793
1201	75-79	-0.1403695194663186
1201	80-84	-0.06415792351143779
1201	85-89	-0.11391314030549182
1201	90-94	-0.2751062625735159
1201	95-99	-0.05689445470614629
1201	100-104	-0.23377098218389847
1201	105-109	-0.16056357736152194
1201	110-114	-0.30805511764430804
1201	115-119	-0.24543036919030214
1201	120-124	-0.25843936676093904
1201	125-129	-0.19068794557185953
1201	130-134	-0.1791001589683674
1201	135-139	-0.23755671435134929
1201	140-144	-0.12713429457348724
1201	145-149	-0.32747581072236187
1201	150-154	-0.25894756992587986
1201	155-159	-0.21864554457073382
1201	160-164	-0.28876097194411443
1201	165-169	-0.2865516022074104
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2404	240-244	0.4012603225067579
2404	245-249	0.5070538169462395
2404	250-254	0.4597312258042052
2404	255-259	0.5612906632031169
2404	260-264	0.5397080593846582
2404	265-269	0.5069937557625082
2404	270-274	0.5827737156469226
2404	275-279	0.8009036025057625
2404	280-284	0.7811197697322818
2404	285-289	0.7721339317284475
2404	290-294	0.8412909617786326
2404	295-299	0.8794217888594673
2404	300-301	1.009388886209166
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	7.0
17	57.0
18	136.0
19	231.0
20	362.0
21	631.0
22	955.0
23	1027.0
24	987.0
25	1074.0
26	1238.0
27	1421.0
28	1929.0
29	2761.0
30	4279.0
31	7151.0
32	21063.0
33	191643.0
34	6244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.22862218128587639	0.06579055576571982	0.10362012533100874	99.6019671376174
2	0.05016777419567077	0.12788670307257055	0.2183531811303375	99.60359234160143
3	0.09046201417786477	0.14638398657872664	99.61306929390285	0.15008470534054835
4	0.08881725028372177	99.60032237372324	0.14802875047286962	0.1628316255201566
5	0.16982187207026433	0.20353953190019575	0.1546078060494416	99.47203078998011
6	99.52671918946035	0.14391684073751213	0.046875770983075375	0.2824881988190595
7	99.84621457589763	0.06743531965986282	0.03412885080346716	0.052221253639040115
8	99.64473099886511	0.1200677642724387	0.09662987878090101	0.13857135808154739
9	30.01652987713614	18.760999358542083	27.86682346749124	23.35564729683054
10-14	59.75098274642675	11.453560091448873	8.61272389348509	20.182733268639286
15-19	34.58479479261327	23.421480059047752	20.342937502312978	21.650787646025996
20-24	59.064274582529784	40.3005867815832	0.5269931864254845	0.10814544946153434
25-29	14.668247868133061	15.230193930193025	59.49448704020632	10.607071161467589
30-34	7.2789252714988875	55.45806313659859	0.6008544864651483	36.66215710543738
35-39	62.64136450103091	7.077456767197103	19.67848134371014	10.602697388061848
40-44	30.80091322398332	52.15525465658699	0.35668699708531537	16.68714512234438
45-49	20.176364203100107	19.925602006097577	26.762716785111845	33.13531700569047
50-54	42.2684990994103	20.504330232672903	15.657019253703112	21.57015141421369
55-59	27.045970649122708	32.70567450842668	2.0619472447466403	38.186407597703976
60-64	39.99740089685508	0.2077637513941392	0.4900460929557725	59.304789258795
65-69	43.764923608397936	23.17065246447739	28.619769282967656	4.444654644157019
70-74	40.18666528481385	54.46400150029734	5.259430528583379	0.0899026863054273
75-79	37.76335941908345	22.14884176966343	23.72254127958912	16.365257531664
80-84	38.588265487424486	0.491734775799213	40.89895829220463	20.02104144457167
85-89	25.343552014424827	15.155114577660934	50.372383567413195	9.128949840501052
90-94	12.550631932585082	59.35954512699525	8.305282173282134	19.784540767137532
95-99	15.103683783176795	3.1222363810916844	57.83109337093432	23.942986464797205
100-104	24.91957049409635	15.512732957584236	38.66663923972518	20.901057308594233
105-109	49.1515382406401	43.121012871538866	4.98585965636949	2.7415892314515475
110-114	34.71456244208877	58.40192162419706	5.085901999799214	1.7976139339149555
115-119	38.06989442908904	34.36289527359048	17.77848381536861	9.788726481951867
120-124	33.6939518187357	20.901299214543297	25.766355447973787	19.638393518747222
125-129	19.216414530301655	35.15189946388425	21.673570563868598	23.9581154419455
130-134	36.60072468364075	44.952048808654034	7.536611020825388	10.910615486879832
135-139	20.035475162031528	19.053111408980175	25.84908473917188	35.06232868981642
140-144	39.520415310452805	17.164434922335296	17.148119154546578	26.16703061266532
145-149	5.412335446606071	40.87303549704768	25.95567072507344	27.758958331272808
150-154	27.788352861744126	13.548922152085005	17.80714069606494	40.85558429010593
155-159	32.77683690817974	45.6854486958558	0.1663882535499734	21.371326142414492
160-164	33.01965363296176	8.71384726008533	4.831607435297378	53.43489167165553
165-169	31.591681434465197	33.55158613990225	26.04701561277224	8.809716812860312
170-174	13.487145693141208	40.511417573834635	21.216389973220025	24.785046759804136
175-179	61.19518093754949	10.071662355487515	14.226808715620546	14.506347991342446
180-184	34.91938697305365	29.492331388302922	5.317003107703756	30.27127853093968
185-189	33.482105284003424	8.707656103280573	37.94773921093932	19.86249940177668
190-194	31.421521092354098	21.89354581804827	17.53238232393465	29.152550765662976
195-199	11.729757971393692	37.20809111626337	35.83860687663994	15.223544035702997
200-204	60.207572215825934	4.037307841192469	27.572681010980027	8.182438932001574
205-209	26.504406806526497	43.9426636330314	17.356857215505883	12.196072344936217
210-214	61.55215432044743	17.699311588399546	19.7406030743217	1.0079310168313251
215-219	42.42379701554654	17.073613821201988	13.223601145821377	27.278988017430095
220-224	45.55064325611397	11.700348449322055	30.400902548990622	12.348105745573354
225-229	34.12315972405773	13.24709586615214	35.533019241951685	17.096725167838454
230-234	21.408816045996762	44.98108250999571	24.47799292865876	9.132108515348776
235-239	55.68608335364368	25.793611123733356	11.58193467367821	6.938370848944757
240-244	30.164056335235134	40.742405526108136	18.595089464616958	10.49844867403977
245-249	27.943865842603223	29.637443486122233	18.025189632931465	24.39350103834308
250-254	41.7306245919118	16.997627922738054	19.121670206874892	22.15007727847526
255-259	8.147499111401341	37.525645370607634	13.617216495697528	40.709639022293494
260-264	32.68268446188988	22.851192902192686	26.7540940780233	17.712028557894136
265-269	24.549350474317876	34.432592124661305	15.731908078328392	25.28614932269243
270-274	16.59316386755107	14.401063137429698	40.014355993916894	28.991417001102338
275-279	16.156405618454254	40.81170337618385	20.18208780675005	22.849803198611845
280-284	23.827743358915157	33.285048572924744	26.252211902538136	16.634996165621967
285-289	32.3586309154425	19.665159104862425	31.725488054517143	16.25072192517794
290-294	36.101495032420644	24.80586923328382	23.20581626783963	15.88681946645591
295-299	45.142621054644835	25.133861188410812	15.239186574387245	14.484331182557103
300-301	47.476668969938146	50.91430165086289	1.3842522831525403	0.22477709604642065
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.5
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	0.5
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	0.5
25	0.5
26	1.0
27	1.25
28	2.75
29	4.083333333333334
30	3.083333333333333
31	1.25
32	0.5
33	1.25
34	1.0833333333333333
35	1.25
36	1.3333333333333333
37	1.75
38	2.0
39	2.25
40	3.0833333333333335
41	2.583333333333333
42	4.833333333333334
43	5.25
44	5.583333333333332
45	10.166666666666668
46	14.166666666666668
47	31.833333333333325
48	150.25
49	803.416666666667
50	2805.0833333333358
51	4142.333333333362
52	15151.833333333412
53	17080.416666666704
54	17209.250000000033
55	15833.333333333369
56	5581.666666666666
57	752.5000000000006
58	387.74999999999983
59	119.24999999999997
60	84.75000000000001
61	48.91666666666667
62	20.916666666666668
63	12.166666666666668
64	9.083333333333332
65	5.25
66	4.083333333333334
67	4.666666666666666
68	4.5
69	5.0
70	4.0
71	3.0
72	2.0
73	1.0
74	0.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004934291682428987
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	8.224117058792568E-5
35-39	8.22423880557735E-5
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.6271978339469383
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
10-19	5.0
20-29	3.0
30-39	5.0
40-49	6.0
50-59	15.0
60-69	8.0
70-79	13.0
80-89	6.0
90-99	55.0
100-109	17.0
110-119	122.0
120-129	117.0
130-139	80.0
140-149	129.0
150-159	72.0
160-169	23.0
170-179	73.0
180-189	90.0
190-199	63.0
200-209	44.0
210-219	84.0
220-229	42.0
230-239	63.0
240-249	65.0
250-259	65.0
260-269	72.0
270-279	74.0
280-289	99.0
290-299	787.0
300-302	240899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.824503692494942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.97332310577005	2.941660224674748
2	12.596948776953889	1.2154805177716739
3	6.392227051904883	0.9251796904554351
4	4.014318588596267	0.774683794141351
5	2.4972300349441747	0.6023947762298723
6	1.7813006051308276	0.5156334808138292
7	1.4233358902241542	0.4806822480632906
8	1.201738685758118	0.46382341814832484
9	0.8182050626438252	0.3552690011348871
>10	6.358135174294724	6.149772200200662
>50	0.8437739708514447	2.8536653563380976
>100	0.5966078581777892	6.712281451997566
>500	0.19602829625841642	6.491883090182404
>1k	0.17045938805079688	18.588299149657068
>5k	0.11079860223301799	37.03761575025905
>10k+	0.025568908207619537	13.891675849931742
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	12800	5.263244461257586	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	10983	4.51611046234313	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	10001	4.112320926331025	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	8776	3.608611983749733	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	8552	3.516505205677725	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	8367	3.4404348755736116	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	7466	3.0699518084179016	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	7388	3.037878912482113	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	7193	2.9576966726426424	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	7142	2.936725932992319	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	6765	2.781706935969342	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	6174	2.538693070609714	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	5785	2.3787397819043075	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	5751	2.364759288804092	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	5597	2.3014358788795866	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	5118	2.104475402555963	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4937	2.0300498363459925	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4811	1.9782397736804882	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4701	1.9330087665915558	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4258	1.750851165315219	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3984	1.6381848385664237	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2230	0.9169558709847201	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	2224	0.9144887251435055	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	1809	0.7438444711261699	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	1784	0.7335646967877761	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	1600	0.6579055576571983	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC	1519	0.6245990888008026	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	1445	0.5941709567591572	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	1438	0.591292619944407	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC	1366	0.5616868698498331	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	1305	0.5366042204641523	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1292	0.5312587378081877	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	1261	0.5185118176285795	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC	1171	0.481504630010362	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC	1056	0.4342176680537509	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC	1015	0.41735883813878516	No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	977	0.4017335811444267	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC	954	0.39227618875310444	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC	880	0.3618480567114591	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC	878	0.36102567476438757	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	839	0.34498922679649335	No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	828	0.34046612608760013	No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	758	0.3116827579400977	No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC	746	0.3067484662576687	No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	688	0.28289938979259527	No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC	681	0.280021052977845	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	665	0.27344199740127306	No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	654	0.2689188966923798	No Hit
CCTACGGGGGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	651	0.26768532377177257	No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	632	0.25987269527459333	No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC	593	0.24383624730669912	No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC	582	0.2393131465978059	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	579	0.23807957367719865	No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	565	0.23232290004769818	No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	551	0.22656622641819765	No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	544	0.22368788960344743	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	516	0.21217454234444644	No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC	514	0.21135216039737498	No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	513	0.21094096942383922	No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	496	0.20395072287373145	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC	477	0.19613809437655222	No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	474	0.194904521455945	No Hit
CCTACGGGAGGCAGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	469	0.19284856658826627	No Hit
CCTACGGGCGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	462	0.18997022977351602	No Hit
CCTACGGGGGGCAGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	462	0.18997022977351602	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	459	0.18873665685290877	No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	436	0.17927926446158654	No Hit
CCTACGGGCGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	428	0.17598973667330056	No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	418	0.17187782693794307	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	408	0.16776591720258557	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	403	0.16570996233490684	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	393	0.16159805259954935	No Hit
CCTACGGGGGGCAGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC	390	0.1603644796789421	No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	387	0.15913090675833483	No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	355	0.14597279560519089	No Hit
CCTACGGGGGGCAGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	325	0.1336370663991184	No Hit
CCTACGGGAGGCAGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	298	0.12253491011365318	No Hit
CCTACGGGGGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	296	0.12171252816658168	No Hit
CCTACGGGGGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC	284	0.1167782364841527	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC	276	0.1134887086958667	No Hit
CCTACGGGTGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC	269	0.11061037188111647	No Hit
CCTACGGGCGGCAGCCGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	267	0.10978798993404497	No Hit
CCTACGGGTGGCTGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC	264	0.10855441701343772	No Hit
CCTACGGGAGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	260	0.10690965311929473	No Hit
CCTACGGGAGGCAGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC	250	0.10279774338393724	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	1.6447638941429957E-4	0.0	0.0	0.0
160-164	0.0	4.1119097353574895E-4	0.0	0.0	0.0
165-169	0.0	4.1119097353574895E-4	0.0	0.0	0.0
170-174	0.0	4.1119097353574895E-4	0.0	0.0	0.0
175-179	0.0	4.1119097353574895E-4	0.0	0.0	0.0
180-184	0.0	4.1119097353574895E-4	0.0	0.0	0.0
185-189	0.0	4.1119097353574895E-4	0.0	0.0	0.0
190-194	0.0	4.1119097353574895E-4	0.0	0.0	0.0
195-199	0.0	4.1119097353574895E-4	0.0	0.0	0.0
200-204	0.0	4.1119097353574895E-4	0.0	0.0	0.0
205-209	0.0	4.1119097353574895E-4	0.0	0.0	0.0
210-214	0.0	4.1119097353574895E-4	0.0	0.0	0.0
215-219	0.0	4.1119097353574895E-4	0.0	0.0	0.0
220-224	0.0	4.1119097353574895E-4	0.0	0.0	0.0
225-229	0.0	4.1119097353574895E-4	0.0	0.0	0.0
230-234	0.0	4.1119097353574895E-4	0.0	0.0	0.0
235-239	0.0	4.1119097353574895E-4	0.0	0.0	0.0
240-244	0.0	4.1119097353574895E-4	0.0	0.0	0.0
245-249	0.0	4.1119097353574895E-4	0.0	0.0	0.0
250-254	0.0	4.1119097353574895E-4	0.0	0.0	0.0
255-259	0.0	4.1119097353574895E-4	0.0	0.0	0.0
260-264	0.0	4.1119097353574895E-4	0.0	0.0	0.0
265-269	0.0	4.1119097353574895E-4	0.0	0.0	0.0
270-274	0.0	4.1119097353574895E-4	0.0	0.0	0.0
275-279	0.0	4.1119097353574895E-4	0.0	0.0	0.0
280-284	0.0	4.1119097353574895E-4	0.0	0.0	0.0
285-289	0.0	4.1119097353574895E-4	0.0	0.0	0.0
290	0.0	4.1119097353574895E-4	0.0	0.0	0.0
>>END_MODULE
