##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	ICMS1_cleaned_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	194122
Sequences flagged as poor quality	0
Sequence length	15-301
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22525525185193	34.0	34.0	34.0	20.0	34.0
2	33.14609369365657	34.0	34.0	34.0	34.0	34.0
3	33.617245855699	34.0	34.0	34.0	34.0	34.0
4	33.48903782157612	34.0	34.0	34.0	34.0	34.0
5	33.285464810789094	34.0	34.0	34.0	34.0	34.0
6	33.15311505135946	34.0	34.0	34.0	34.0	34.0
7	33.55889595203017	34.0	34.0	34.0	34.0	34.0
8	33.493704989645686	34.0	34.0	34.0	34.0	34.0
9	33.40864507886793	34.0	34.0	34.0	34.0	34.0
10-14	33.271244887235866	34.0	34.0	34.0	31.2	34.0
15-19	33.43765433796379	34.0	34.0	34.0	34.0	34.0
20-24	33.64373297136315	34.0	34.0	34.0	34.0	34.0
25-29	33.56199140711128	34.0	34.0	34.0	34.0	34.0
30-34	33.48794178706951	34.0	34.0	34.0	34.0	34.0
35-39	33.55188381724892	34.0	34.0	34.0	34.0	34.0
40-44	33.45129420725065	34.0	34.0	34.0	34.0	34.0
45-49	33.518884916178095	34.0	34.0	34.0	34.0	34.0
50-54	33.47165035810078	34.0	34.0	34.0	34.0	34.0
55-59	33.42781484068727	34.0	34.0	34.0	34.0	34.0
60-64	33.32817187187963	34.0	34.0	34.0	34.0	34.0
65-69	33.40324655563794	34.0	34.0	34.0	34.0	34.0
70-74	33.336709972890844	34.0	34.0	34.0	34.0	34.0
75-79	33.426107675493505	34.0	34.0	34.0	34.0	34.0
80-84	33.56816901369229	34.0	34.0	34.0	34.0	34.0
85-89	33.54157138080261	34.0	34.0	34.0	34.0	34.0
90-94	33.27243646820168	34.0	34.0	34.0	34.0	34.0
95-99	33.30583422552668	34.0	34.0	34.0	34.0	34.0
100-104	33.234493239750066	34.0	34.0	34.0	34.0	34.0
105-109	33.41922234125559	34.0	34.0	34.0	34.0	34.0
110-114	33.32965997672043	34.0	34.0	34.0	34.0	34.0
115-119	33.33018238174343	34.0	34.0	34.0	34.0	34.0
120-124	33.1674561297122	34.0	34.0	34.0	34.0	34.0
125-129	33.13089439121023	34.0	34.0	34.0	34.0	34.0
130-134	33.274424443615274	34.0	34.0	34.0	34.0	34.0
135-139	33.12735238001311	34.0	34.0	34.0	34.0	34.0
140-144	33.31022800978653	34.0	34.0	34.0	34.0	34.0
145-149	33.24512632482473	34.0	34.0	34.0	34.0	34.0
150-154	33.165081164967155	34.0	34.0	34.0	34.0	34.0
155-159	33.03854257508569	34.0	34.0	34.0	34.0	34.0
160-164	33.323804385248295	34.0	34.0	34.0	34.0	34.0
165-169	33.36784578350496	34.0	34.0	34.0	34.0	34.0
170-174	32.88528620946779	34.0	34.0	34.0	31.2	34.0
175-179	33.291925365823666	34.0	34.0	34.0	34.0	34.0
180-184	33.18156315994973	34.0	34.0	34.0	34.0	34.0
185-189	33.11859436667469	34.0	34.0	34.0	34.0	34.0
190-194	33.27878848423438	34.0	34.0	34.0	34.0	34.0
195-199	32.80928617553494	34.0	34.0	34.0	31.2	34.0
200-204	33.07582824125046	34.0	34.0	34.0	34.0	34.0
205-209	32.54167701558332	34.0	34.0	34.0	28.4	34.0
210-214	32.52090607786384	34.0	34.0	34.0	31.2	34.0
215-219	33.12645119953094	34.0	34.0	34.0	34.0	34.0
220-224	33.138419173450714	34.0	34.0	34.0	34.0	34.0
225-229	33.26584745816696	34.0	34.0	34.0	34.0	34.0
230-234	32.76431143972279	34.0	34.0	34.0	31.2	34.0
235-239	32.98532527873724	34.0	34.0	34.0	34.0	34.0
240-244	32.84825611166731	34.0	34.0	34.0	34.0	34.0
245-249	32.8376699948155	34.0	34.0	34.0	34.0	34.0
250-254	32.82838395870156	34.0	34.0	34.0	34.0	34.0
255-259	32.54015688213123	34.0	34.0	34.0	31.2	34.0
260-264	32.75746092934777	34.0	34.0	34.0	34.0	34.0
265-269	32.83844551436223	34.0	34.0	34.0	34.0	34.0
270-274	32.534216915156435	34.0	34.0	34.0	34.0	34.0
275-279	32.456856591624714	34.0	34.0	34.0	28.4	34.0
280-284	32.24310677306396	34.0	34.0	34.0	28.4	34.0
285-289	32.2766189100869	34.0	34.0	34.0	31.2	34.0
290-294	31.904166929096068	34.0	34.0	34.0	25.6	34.0
295-299	31.897903946055585	34.0	34.0	34.0	20.0	34.0
300-301	31.384789482078844	34.0	34.0	34.0	20.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.15304782792846794
1101	2	-0.01440808851000952
1101	3	0.14780290695122034
1101	4	0.2108825119285811
1101	5	0.021187523711596157
1101	6	-0.30468668010257716
1101	7	0.01030546178660785
1101	8	-0.03988537243171919
1101	9	-0.11549498016945137
1101	10-14	0.052216844436188126
1101	15-19	-0.07044513975615985
1101	20-24	-0.017240791817137335
1101	25-29	-0.02880869463615454
1101	30-34	0.056582152050793866
1101	35-39	-0.04329143312146044
1101	40-44	-0.04625204817421036
1101	45-49	0.06636558870417275
1101	50-54	-0.053502438622018644
1101	55-59	-0.022391366251461875
1101	60-64	-0.0727034428318376
1101	65-69	-0.109714594086185
1101	70-74	-0.16371604772617587
1101	75-79	-0.050130381859418094
1101	80-84	-0.09088161413608731
1101	85-89	-0.017697257877792083
1101	90-94	-0.06730900593580458
1101	95-99	-0.045008582911059136
1101	100-104	0.04589555666261447
1101	105-109	-0.05693171416568532
1101	110-114	-0.09403148708097575
1101	115-119	0.055460547321217746
1101	120-124	-0.019501893232849454
1101	125-129	-0.1776739977877071
1101	130-134	0.015513956993487454
1101	135-139	-0.008799755564041334
1101	140-144	0.10713684831416259
1101	145-149	0.052451772695221166
1101	150-154	0.06553513373248165
1101	155-159	-0.09806799403156674
1101	160-164	-0.04977284916693492
1101	165-169	-0.010906937580315912
1101	170-174	-0.12948765825631625
1101	175-179	-0.018234010863814376
1101	180-184	-0.037021614735024855
1101	185-189	-0.16928052252664827
1101	190-194	0.06015799833304669
1101	195-199	-0.12287527955110278
1101	200-204	0.21045933496944969
1101	205-209	-0.12902219637953039
1101	210-214	-0.17732757846148672
1101	215-219	-0.1270403017377717
1101	220-224	-0.09913247440094608
1101	225-229	-0.035590888733963766
1101	230-234	-0.27853691501187683
1101	235-239	-0.14623158257264635
1101	240-244	-0.3286880626385269
1101	245-249	-0.11722564042590022
1101	250-254	-0.26192419868417005
1101	255-259	-0.3341531283161103
1101	260-264	-0.1283616148519613
1101	265-269	-0.26468266285242237
1101	270-274	-0.21373829229610664
1101	275-279	-0.3620711768264826
1101	280-284	-0.388395687489826
1101	285-289	-0.4530467846806374
1101	290-294	-0.589002108575265
1101	295-299	-0.7135336981152989
1101	300-301	-0.5076373911582621
1102	1	0.36443986707098475
1102	2	0.008051554132968874
1102	3	-0.10003916599663398
1102	4	-0.14245848235437109
1102	5	0.11235202787216281
1102	6	-0.001580650807802897
1102	7	0.0330207348691971
1102	8	0.12411947260127931
1102	9	0.015072447734404193
1102	10-14	-0.016385287091651435
1102	15-19	-0.0017460120054479944
1102	20-24	0.01659751117899333
1102	25-29	0.012872923623987731
1102	30-34	-0.11049216951318641
1102	35-39	0.07926926716260851
1102	40-44	-0.028744946665035798
1102	45-49	-0.023875778990351648
1102	50-54	-0.12259719628674759
1102	55-59	-0.11059676889328784
1102	60-64	-0.0023816590569651908
1102	65-69	-0.12388416546321679
1102	70-74	-0.26161103856409085
1102	75-79	-0.06173313693440008
1102	80-84	-0.07964390888515993
1102	85-89	0.009957655363137974
1102	90-94	-0.033219134664840055
1102	95-99	-0.10698886621887027
1102	100-104	-0.0944379999989593
1102	105-109	-0.0945145051304479
1102	110-114	-0.18607733317591624
1102	115-119	-0.045181680856423156
1102	120-124	-0.10077247133392575
1102	125-129	-0.15163743576626842
1102	130-134	0.008563344436062437
1102	135-139	-0.09656631375700186
1102	140-144	-0.05271409769282798
1102	145-149	-0.07952128052969698
1102	150-154	-0.10184881946774027
1102	155-159	-0.3301433798157305
1102	160-164	-0.05730184139491712
1102	165-169	-0.10860697137162845
1102	170-174	-0.20410702667030733
1102	175-179	-0.21566203656438176
1102	180-184	-0.303112151489664
1102	185-189	-0.12821904390504812
1102	190-194	-0.16028311011789498
1102	195-199	-0.3250230650217958
1102	200-204	-0.057094054107572845
1102	205-209	-0.4066655278675242
1102	210-214	-0.15372446582617272
1102	215-219	-0.21836839621924042
1102	220-224	-0.16645579496067597
1102	225-229	-0.17141605177636166
1102	230-234	-0.259243756356625
1102	235-239	-0.15728689996475964
1102	240-244	-0.3396440074699498
1102	245-249	-0.3877393533592155
1102	250-254	-0.38566175911181233
1102	255-259	-0.4406037036688346
1102	260-264	-0.45885986983139304
1102	265-269	-0.5493648866855168
1102	270-274	-0.6080942188852205
1102	275-279	-0.5264655562760439
1102	280-284	-0.5287414271913278
1102	285-289	-0.5159236923217634
1102	290-294	-0.6360058098431054
1102	295-299	-0.9874185223682446
1102	300-301	-0.8680416439842666
1103	1	-0.2569537228498291
1103	2	0.09705410157663863
1103	3	-0.2588693166710101
1103	4	-0.05986157088297972
1103	5	-0.16549952922809297
1103	6	-0.03568479978389405
1103	7	-0.574376725586184
1103	8	-0.39752392478018095
1103	9	-0.35843166964547635
1103	10-14	-0.23307499265454368
1103	15-19	-0.1363260787524183
1103	20-24	0.006347999932728499
1103	25-29	-0.043909398301806846
1103	30-34	0.0030764238634617413
1103	35-39	-0.04893626612201274
1103	40-44	-0.15631756855725598
1103	45-49	-0.08011899074413265
1103	50-54	-0.12060219243596748
1103	55-59	-0.1515755599436588
1103	60-64	-0.11481821981053741
1103	65-69	-0.06738028505483129
1103	70-74	-0.1043231774293929
1103	75-79	-0.21178448076030065
1103	80-84	-0.0811412725771703
1103	85-89	-0.09028918995370816
1103	90-94	-0.11165544980732278
1103	95-99	-0.17819991107433708
1103	100-104	-0.020969794769158057
1103	105-109	0.009145855408370096
1103	110-114	-0.12209506296989758
1103	115-119	-0.10263314678352486
1103	120-124	-0.21195462268098453
1103	125-129	-0.1380627212793044
1103	130-134	-0.12562316350044256
1103	135-139	-0.15018238518557325
1103	140-144	-0.10943574703331649
1103	145-149	-0.17376228209464273
1103	150-154	-0.13934490710467173
1103	155-159	-0.2546906635179411
1103	160-164	-0.11025511660172782
1103	165-169	-0.1830268238196453
1103	170-174	-0.1607893427892364
1103	175-179	-0.1937215044830083
1103	180-184	-0.22014322683060783
1103	185-189	-0.30226624532590307
1103	190-194	-0.19052163364556662
1103	195-199	-0.3788074210431063
1103	200-204	0.045072238116709684
1103	205-209	-0.44767008315394463
1103	210-214	-0.24745272355371384
1103	215-219	-0.22430671544813663
1103	220-224	-0.4262127798993305
1103	225-229	-0.22917708083120658
1103	230-234	-0.454574507310042
1103	235-239	-0.42726473675569565
1103	240-244	-0.4487249331333416
1103	245-249	-0.3220763539382503
1103	250-254	-0.5016921486651498
1103	255-259	-0.4640819753518812
1103	260-264	-0.5051723595195625
1103	265-269	-0.3554969880989276
1103	270-274	-0.7082812067310122
1103	275-279	-0.48498175272327515
1103	280-284	-0.36702993843809395
1103	285-289	-0.46518949004491006
1103	290-294	-0.31775249630848634
1103	295-299	-0.57762241588377
1103	300-301	-0.770892696031332
1104	1	-0.07044080988983836
1104	2	0.22524315201360423
1104	3	0.053882966120852416
1104	4	0.009587227957013056
1104	5	0.03203054875913125
1104	6	0.1736462344285954
1104	7	-0.011634240184292821
1104	8	0.07000752484163542
1104	9	-0.014636356716842158
1104	10-14	-0.10562858437771894
1104	15-19	0.06761639097364736
1104	20-24	-0.010477057871874251
1104	25-29	-0.08192719036200202
1104	30-34	-0.05302343113516628
1104	35-39	-0.03326436111751008
1104	40-44	-0.07657269259220811
1104	45-49	-0.11638892843273396
1104	50-54	-0.08900658950458507
1104	55-59	-0.15997878132549204
1104	60-64	-0.02131878805915477
1104	65-69	-0.18610505677564504
1104	70-74	-0.21400691768099733
1104	75-79	-0.21399281246074509
1104	80-84	-0.05839663174948839
1104	85-89	-0.10009049888500954
1104	90-94	-0.04792146853993273
1104	95-99	-0.393314330615155
1104	100-104	-0.2199885030949318
1104	105-109	-0.1246586614771985
1104	110-114	-0.13372729447623044
1104	115-119	-0.1942509533022374
1104	120-124	-0.19090506277318298
1104	125-129	-0.38550855979727316
1104	130-134	-0.26207588951839256
1104	135-139	-0.26844325475078534
1104	140-144	-0.3500982729132289
1104	145-149	-0.2605669749862898
1104	150-154	-0.27750225700115294
1104	155-159	-0.4846768608954406
1104	160-164	-0.23023388675156298
1104	165-169	-0.2968326447483278
1104	170-174	-0.47363537700385905
1104	175-179	-0.30021191402163083
1104	180-184	-0.2581634198741227
1104	185-189	-0.39447682908163983
1104	190-194	-0.14047529715598017
1104	195-199	-0.31469735165541124
1104	200-204	0.05720691264830435
1104	205-209	-0.4266807385500968
1104	210-214	-0.15882443218715991
1104	215-219	-0.2509104815453185
1104	220-224	-0.2517599354839817
1104	225-229	-0.12768393639799314
1104	230-234	-0.2793884843324861
1104	235-239	-0.23594977753193547
1104	240-244	-0.2752812677243668
1104	245-249	-0.3521557481968216
1104	250-254	-0.30197981622280423
1104	255-259	-0.4415671277857385
1104	260-264	-0.5123964188168699
1104	265-269	-0.45959967852233063
1104	270-274	-0.5849695948407003
1104	275-279	-0.5584937841136188
1104	280-284	-0.6959092842817824
1104	285-289	-0.8108698130114185
1104	290-294	-0.8089446813164614
1104	295-299	-1.1536582970639984
1104	300-301	-0.933392347819634
1201	1	-0.22827856559270288
1201	2	-0.21821731257261945
1201	3	-0.05041626083502848
1201	4	0.12454667637958039
1201	5	-0.193097183961342
1201	6	-0.1914081825593854
1201	7	-0.024132103391572457
1201	8	0.16015643555219583
1201	9	0.0429525813446574
1201	10-14	-0.15803033939680944
1201	15-19	-0.12355912992893536
1201	20-24	-0.08131411080736228
1201	25-29	-0.1408846254509868
1201	30-34	-0.04902810358859
1201	35-39	-0.11522888086147276
1201	40-44	-0.2014639535848275
1201	45-49	-0.1557771409343971
1201	50-54	-0.25195460371188005
1201	55-59	-0.17271596531758604
1201	60-64	-0.26157406356296065
1201	65-69	-0.24760859910313826
1201	70-74	-0.21710954071276944
1201	75-79	-0.23484885910929876
1201	80-84	-0.15210306492010517
1201	85-89	-0.12703687963298194
1201	90-94	-0.32218973574349263
1201	95-99	-0.3243700011881927
1201	100-104	-0.23065157361858724
1201	105-109	-0.2901375879525432
1201	110-114	-0.4338744957526117
1201	115-119	-0.35652753975212903
1201	120-124	-0.4401263823768673
1201	125-129	-0.35383452165583407
1201	130-134	-0.256459661698905
1201	135-139	-0.3936004395238655
1201	140-144	-0.21308459307480376
1201	145-149	-0.24403494739797793
1201	150-154	-0.28671487047463984
1201	155-159	-0.4479018967291708
1201	160-164	-0.2759742863208885
1201	165-169	-0.2809608897537146
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2404	295-299	0.8791750016048958
2404	300-301	0.7036614325083832
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	13.0
17	49.0
18	119.0
19	134.0
20	247.0
21	487.0
22	695.0
23	758.0
24	764.0
25	783.0
26	899.0
27	1160.0
28	1597.0
29	2198.0
30	3273.0
31	5659.0
32	16237.0
33	153696.0
34	5354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.22614760896554212	0.05769597313016109	0.0978770972743804	99.61827932062992
2	0.041728916593683996	0.13652052959662048	0.2364638606975426	99.58528669311215
3	0.0932403333985844	0.15402684909489908	99.58325176950578	0.16948104800074182
4	0.10199771277856194	99.55543421147526	0.17308702774543844	0.16948104800074182
5	0.18545038687011262	0.21275280493710141	0.14681488960550582	99.45498191858728
6	99.51473815435654	0.14990572938667437	0.04893829653516861	0.2864178197216184
7	99.83927633137924	0.05769567591514614	0.044302036863415786	0.058725955842202325
8	99.63682632571269	0.11745191168440465	0.08551323394566303	0.16020852865723617
9	30.758492082298762	20.985771834207352	25.77142209538332	22.484313988110568
10-14	59.74170882228702	11.21603939790441	8.843716837864848	20.198534941943727
15-19	35.42082650758817	24.410822283353426	19.88275172833019	20.285599480728216
20-24	59.72657529843042	39.7422978884747	0.418204410155598	0.11292240293927949
25-29	15.090616854013621	15.365712931576288	59.41652843174629	10.127141782663795
30-34	0.8172550862074295	59.29035222746315	0.39750001545489955	39.49489267087452
35-39	56.23274805959139	5.067678608778797	19.80427451436979	18.895298817260027
40-44	38.72070906172142	46.494669878683844	0.26192090442050775	14.522700155174226
45-49	20.21926615902979	19.92993374617469	24.925657643919177	34.92514245087634
50-54	44.57709166671819	20.20393737622283	19.514494680028935	15.70447627703005
55-59	20.348345959491876	31.135880272692052	8.61745624809371	39.89831751972236
60-64	39.989489303820946	0.14838629899839248	0.5149210667326162	59.34720333044804
65-69	31.958158409434244	20.244388523755642	42.02511896886097	5.772334097949139
70-74	40.061355256622434	54.03085693322643	5.784232296731086	0.12355551342004548
75-79	39.98555224532741	19.91533327218972	11.736378972230838	28.36273551025203
80-84	40.00950170659719	0.2722723300410573	39.607791811631245	20.110434151730505
85-89	33.91496216200618	6.12436502971697	49.16558540936453	10.795087398912319
90-94	19.93692639867257	59.30114037483446	9.111868040132125	11.650065186360848
95-99	11.395798504678291	8.67560753886208	59.50860933432004	20.419984622139584
100-104	27.913212639548533	16.44324811366563	20.919522929310528	34.72401631747531
105-109	57.96090220602942	36.86630006041374	4.836604541546303	0.33619319201054043
110-114	47.74896555421857	46.07652991257348	5.8699191518627245	0.30458538134522806
115-119	35.28934877186902	38.655646803270066	19.2456695154932	6.809334909367709
120-124	30.33897623132546	28.5409795306102	34.070015402852164	7.050028835212179
125-129	18.141048660384445	41.71052251404419	24.728403316946725	15.42002550862464
130-134	30.584381867862454	20.738118759245324	30.314544874593928	18.362954498298294
135-139	7.224227030319925	15.297391340261537	36.208073267561865	41.27030836185668
140-144	39.74661111994663	19.371608796320203	19.94952050677776	20.93225957695541
145-149	5.146776292684942	32.12386638296114	33.029004069659344	29.700353254694566
150-154	30.141966329743713	18.47969371141433	7.587171582766515	43.79116837607544
155-159	26.384944319148033	65.59730227589478	0.17542645261471165	7.84232695234248
160-164	26.850443651872762	13.57301968433973	4.890605821406875	54.685930842380635
165-169	33.74355657454122	26.938865337186556	25.688874377871706	13.628703710400508
170-174	7.265060153817698	30.32955178353512	20.78316209756667	41.62222596508051
175-179	57.247196003827526	1.501251387272844	14.376590067849785	26.87496254104984
180-184	34.40589071179434	33.82911768505361	5.7171941609611165	26.04779744219093
185-189	34.35624990310027	5.994139504596923	19.140357314508087	40.50925327779471
190-194	26.35474523935788	32.868769505426634	28.20462723766461	12.571858017550877
195-199	22.503003446985286	40.54363941266845	17.882244200400734	19.071112939945532
200-204	72.4911619235483	0.5369430555295459	20.230195738164053	6.741699282758104
205-209	20.85830634386853	45.15533936408271	6.973932933765164	27.012421358283596
210-214	42.507596237974774	18.88417521092617	37.72005303404811	0.8881755170509429
215-219	40.13158098870878	20.124072122351084	15.21703830038982	24.527308588550323
220-224	20.663764275348605	22.07358585999634	47.49019693025055	9.772452934404507
225-229	42.34744756300922	1.5119728887619948	36.80334007803998	19.33723947018881
230-234	20.265616092979084	52.828158575104254	20.728173369488186	6.178051962428472
235-239	49.38573311619649	25.429117719483756	2.882305347650018	22.302843816669736
240-244	33.97825585329789	32.26989065841905	7.6680866827190215	26.083766805564036
245-249	50.76466113666823	13.046380576354858	16.35982592406688	19.82913236291003
250-254	26.729383660115158	15.339821702674254	45.10582845739588	12.824966179814707
255-259	13.804829169625462	39.55236086066295	15.282398560731062	31.360411408980525
260-264	39.035720238569745	28.252873158626517	31.000504180975085	1.7109024218286553
265-269	21.10913584439546	53.08402177187982	13.835932298854978	11.970910084869745
270-274	11.233531495577852	7.852692379779788	31.194347768008306	49.71942835663405
275-279	40.17115615850432	15.451695456790993	19.351334108710986	25.025814275993707
280-284	12.430509735110931	43.47772945123536	8.520306642787668	35.57145417086603
285-289	45.68994719847345	26.33373838330158	20.85934821140915	7.116966206815821
290-294	27.442285496414147	20.235250968211176	20.261878070664395	32.060585464710286
295-299	36.89833418345131	26.502842711586073	22.155940665911928	14.442882439050688
300-301	30.458981317190272	48.52833733430748	20.944577810249452	0.06810353825279197
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.5
1	0.25
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	0.5
18	0.0
19	0.0
20	0.0
21	0.3333333333333333
22	0.3333333333333333
23	0.0
24	0.5
25	1.25
26	1.5
27	0.75
28	1.25
29	1.1666666666666665
30	0.75
31	1.4166666666666665
32	1.4166666666666665
33	0.25
34	1.3333333333333333
35	1.0
36	1.0
37	1.25
38	1.25
39	1.0
40	1.75
41	2.0
42	3.9166666666666665
43	6.833333333333332
44	3.8333333333333335
45	9.250000000000002
46	16.833333333333336
47	17.16666666666667
48	27.66666666666666
49	71.25000000000001
50	1857.8333333333314
51	2390.4166666666656
52	10487.33333333334
53	2727.5833333333344
54	11414.583333333348
55	30638.583333333132
56	3728.5000000000186
57	233.83333333333368
58	88.16666666666664
59	78.25
60	80.91666666666667
61	49.75000000000001
62	15.750000000000002
63	7.083333333333333
64	5.5
65	4.5
66	3.5
67	3.25
68	3.25
69	3.5
70	2.5
71	2.0
72	1.5
73	1.0
74	1.0
75	1.0
76	1.0
77	1.5
78	1.5
79	1.0
80	1.0
81	1.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.3333333333333333
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.151399635280906E-4
2	0.006181679562337087
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	1.030325572577679E-4
35-39	1.0303499892843601E-4
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.617070774957349
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	1.0344631394921821E-4
230-234	0.0
235-239	0.0
240-244	0.0
245-249	1.0349331329702787E-4
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	1.0367281688374026E-4
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
10-19	6.0
20-29	2.0
30-39	9.0
40-49	4.0
50-59	12.0
60-69	6.0
70-79	7.0
80-89	12.0
90-99	60.0
100-109	9.0
110-119	122.0
120-129	103.0
130-139	86.0
140-149	83.0
150-159	24.0
160-169	16.0
170-179	30.0
180-189	51.0
190-199	42.0
200-209	43.0
210-219	36.0
220-229	36.0
230-239	29.0
240-249	61.0
250-259	42.0
260-269	34.0
270-279	62.0
280-289	44.0
290-299	1063.0
300-302	191988.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.034061054388478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.093602349636065	2.303190776934093
2	11.799259353850083	0.9519786525999114
3	6.12948537862342	0.7418015474804505
4	4.418337377091048	0.7129537095228774
5	3.498914570297536	0.7057417500334842
6	2.4134848678329717	0.5841687186408547
7	1.9793129868471462	0.5589268604279783
8	1.455752777423062	0.4698076467376186
9	1.4685225386285277	0.5331698622515738
>10	8.77282594815477	6.513944838812706
>50	0.2809347465202401	0.7268624885381358
>100	0.2809347465202401	2.1718300862344297
>500	0.06384880602732729	2.155345607401531
>1k	0.12769761205465457	16.53238684950701
>5k	0.2043161792874473	57.901216760593854
>10k+	0.012769761205465456	6.436673844283492
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	12495	6.436673844283492	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9633	4.962343268666096	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9310	4.795953060446523	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9057	4.665622649673916	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8997	4.634714251862231	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8198	4.223117421003287	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	8042	4.142755586692905	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	7699	3.9660625792027693	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	6698	3.4504074757111507	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	6257	3.2232307517952625	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	6031	3.1068091200379144	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	6007	3.0944457609132403	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	5765	2.9697818897394423	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	5237	2.6977879889966103	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	5233	2.695727429142498	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	5146	2.6509102523155543	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	5089	2.621547274394453	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4943	2.546336839719352	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4678	2.409824749384408	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4543	2.3402808543081153	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4344	2.2377680015660255	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4239	2.183678305395576	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3261	1.6798714210651033	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	2565	1.3213340064495525	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1421	0.7320138881734167	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1097	0.5651085399903153	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1002	0.5161702434551467	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	949	0.48886782538815793	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	948	0.4883526854246299	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	881	0.4538383078682478	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	869	0.4476566283059107	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	537	0.2766301604145846	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	329	0.16948104800074182	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	296	0.15248142920431482	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	266	0.13702723029847208	No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	265	0.136512090334944	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	256	0.1318758306631912	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	256	0.1318758306631912	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	237	0.12208817135615747	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	233	0.12002761150204509	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	231	0.11899733157498893	No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	208	0.10714911241384284	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
