##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	ICFS1_cleaned_R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	139294
Sequences flagged as poor quality	0
Sequence length	15-301
%GC	52
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.84479589932086	34.0	34.0	34.0	20.0	34.0
2	32.42196361652333	34.0	34.0	34.0	34.0	34.0
3	32.52063979783767	34.0	34.0	34.0	34.0	34.0
4	33.278884948382554	34.0	34.0	34.0	34.0	34.0
5	32.488125834565736	34.0	34.0	34.0	34.0	34.0
6	32.76816661162721	34.0	34.0	34.0	34.0	34.0
7	32.38051172340517	34.0	34.0	34.0	34.0	34.0
8	32.47269803437334	34.0	34.0	34.0	34.0	34.0
9	32.89617643258145	34.0	34.0	34.0	34.0	34.0
10-14	33.037568021594616	34.0	34.0	34.0	34.0	34.0
15-19	32.994333973001986	34.0	34.0	34.0	34.0	34.0
20-24	32.845656786164845	34.0	34.0	34.0	34.0	34.0
25-29	32.94976485462536	34.0	34.0	34.0	34.0	34.0
30-34	32.63448389326892	34.0	34.0	34.0	31.2	34.0
35-39	32.616025935099906	34.0	34.0	34.0	31.2	34.0
40-44	32.78755075518437	34.0	34.0	34.0	34.0	34.0
45-49	32.69558838987122	34.0	34.0	34.0	34.0	34.0
50-54	32.6489960265969	34.0	34.0	34.0	34.0	34.0
55-59	32.58039224599952	34.0	34.0	34.0	34.0	34.0
60-64	32.49085219793138	34.0	34.0	34.0	34.0	34.0
65-69	31.905274853897556	34.0	34.0	34.0	20.0	34.0
70-74	31.83373462115402	34.0	34.0	34.0	25.6	34.0
75-79	32.52041863305436	34.0	34.0	34.0	34.0	34.0
80-84	32.2671500673477	34.0	34.0	34.0	28.4	34.0
85-89	32.29839659162497	34.0	34.0	34.0	31.2	34.0
90-94	32.26651972603771	34.0	34.0	34.0	28.4	34.0
95-99	31.653970113064883	34.0	34.0	34.0	20.0	34.0
100-104	32.02995614774268	34.0	34.0	34.0	20.0	34.0
105-109	31.872867077442	34.0	34.0	34.0	20.0	34.0
110-114	32.194113565845136	34.0	34.0	34.0	22.8	34.0
115-119	31.92513934180882	34.0	34.0	34.0	20.0	34.0
120-124	31.743335223059965	34.0	34.0	34.0	20.0	34.0
125-129	31.476716825848587	34.0	34.0	34.0	20.0	34.0
130-134	31.77707181386223	34.0	34.0	34.0	22.8	34.0
135-139	31.531288016948803	34.0	34.0	34.0	20.0	34.0
140-144	31.707125955361818	34.0	34.0	34.0	20.0	34.0
145-149	31.19519521030879	34.0	34.0	34.0	20.0	34.0
150-154	31.310615939771946	34.0	34.0	34.0	20.0	34.0
155-159	31.201047719233106	34.0	34.0	34.0	20.0	34.0
160-164	31.057526456820153	34.0	34.0	34.0	20.0	34.0
165-169	31.235490086862207	34.0	34.0	34.0	20.0	34.0
170-174	30.66341797660207	34.0	34.0	34.0	20.0	34.0
175-179	31.060495871924225	34.0	34.0	34.0	20.0	34.0
180-184	31.03142057658412	34.0	34.0	34.0	20.0	34.0
185-189	30.425396130848913	34.0	34.0	34.0	20.0	34.0
190-194	30.361865345489868	34.0	31.2	34.0	17.8	34.0
195-199	30.924875435590604	34.0	34.0	34.0	20.0	34.0
200-204	29.790041692855805	34.0	31.2	34.0	17.8	34.0
205-209	29.78559913026956	34.0	31.2	34.0	20.0	34.0
210-214	29.466953124740268	34.0	28.4	34.0	17.8	34.0
215-219	29.294825475050157	34.0	22.8	34.0	20.0	34.0
220-224	29.59171025944882	34.0	25.6	34.0	20.0	34.0
225-229	28.834154029667957	34.0	22.8	34.0	15.6	34.0
230-234	29.517338698519	34.0	22.8	34.0	20.0	34.0
235-239	28.6522213170309	34.0	20.0	34.0	15.6	34.0
240-244	27.470846845727408	34.0	20.0	34.0	13.4	34.0
245-249	27.857391836621826	34.0	20.0	34.0	15.6	34.0
250-254	27.81934811097396	34.0	20.0	34.0	15.6	34.0
255-259	26.86618748270188	34.0	20.0	34.0	11.2	34.0
260-264	27.474710371914757	34.0	20.0	34.0	11.2	34.0
265-269	26.87164777267538	34.0	20.0	34.0	11.2	34.0
270-274	26.874818393609722	34.0	20.0	34.0	13.4	34.0
275-279	25.14725305837498	28.4	20.0	34.0	9.0	34.0
280-284	25.811085369641024	34.0	20.0	34.0	9.0	34.0
285-289	25.859809980759216	28.4	20.0	34.0	11.2	34.0
290-294	24.640866216997544	20.0	20.0	34.0	9.0	34.0
295-299	24.645071457706287	20.0	20.0	34.0	9.0	34.0
300-301	21.883697730341325	20.0	14.5	27.0	9.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.5641951538305499
1101	2	-0.615862867037027
1101	3	-0.5507495971534446
1101	4	-0.48368032702080654
1101	5	-0.454266443800293
1101	6	-0.5410884425291727
1101	7	-0.17924801267370327
1101	8	-0.7545195333640144
1101	9	-0.30302773676511663
1101	10-14	-0.3163892157717356
1101	15-19	-0.1652600450368169
1101	20-24	-0.3331104270206211
1101	25-29	-0.2029289587247689
1101	30-34	-0.46579874766144513
1101	35-39	-0.48114817177476965
1101	40-44	-0.36654354358564945
1101	45-49	-0.3885294078253594
1101	50-54	-0.5485151394359207
1101	55-59	-0.32177350608691313
1101	60-64	-0.19867269557315836
1101	65-69	-0.9247625078752861
1101	70-74	-0.6638022422964731
1101	75-79	-0.5262701794679998
1101	80-84	-0.5930891219875178
1101	85-89	-0.608321567180333
1101	90-94	-0.7158576938451802
1101	95-99	-0.5747052375136192
1101	100-104	-0.5465625530842964
1101	105-109	-0.7137321976611837
1101	110-114	-0.5953704579524235
1101	115-119	-0.7455159219672325
1101	120-124	-0.8413270759595264
1101	125-129	-0.7729213977186937
1101	130-134	-0.8328503661505273
1101	135-139	-1.1920848746196882
1101	140-144	-0.7407023311293344
1101	145-149	-1.066737454028413
1101	150-154	-0.8479958941310635
1101	155-159	-1.2716934343558535
1101	160-164	-1.4008830538746615
1101	165-169	-1.287531768809398
1101	170-174	-1.3949826306689843
1101	175-179	-1.3592758196606773
1101	180-184	-1.3750709487104196
1101	185-189	-1.7747588260768978
1101	190-194	-1.624593301752526
1101	195-199	-1.2621711421645436
1101	200-204	-1.6207893615078248
1101	205-209	-1.790857880077894
1101	210-214	-1.1640697451094368
1101	215-219	-2.1014917559284036
1101	220-224	-1.9470749146233892
1101	225-229	-2.0956820039192294
1101	230-234	-2.3389153691588582
1101	235-239	-2.4748504495872368
1101	240-244	-2.1137512527864146
1101	245-249	-2.6664996532703427
1101	250-254	-2.75261666283318
1101	255-259	-2.8707158267560082
1101	260-264	-2.7379760292219046
1101	265-269	-3.1817872226250152
1101	270-274	-3.29403098230323
1101	275-279	-3.1884695597402555
1101	280-284	-3.4336298348993424
1101	285-289	-3.385729129204009
1101	290-294	-3.8467938515184557
1101	295-299	-3.7651781006256755
1101	300-301	-1.9390522065021152
1102	1	0.08699039896790595
1102	2	0.054015820583593666
1102	3	-0.35532638237538805
1102	4	-0.011678672761092912
1102	5	0.25160617820135656
1102	6	-0.5523511941145074
1102	7	-0.22706990404398653
1102	8	-0.5778032389057834
1102	9	-0.3505325616892989
1102	10-14	-0.20675866710892166
1102	15-19	-0.20118229483003347
1102	20-24	-0.4796971378008763
1102	25-29	-0.29300064331258824
1102	30-34	-0.43108135036340656
1102	35-39	-0.5689921199412993
1102	40-44	-0.3164222312062748
1102	45-49	-0.3160452611476643
1102	50-54	-0.5211178413934618
1102	55-59	-0.5290136494560826
1102	60-64	-0.56400492606668
1102	65-69	-0.7466924775471959
1102	70-74	-0.6283376710254558
1102	75-79	-0.6386054427710093
1102	80-84	-0.703552314708773
1102	85-89	-0.6936510405743306
1102	90-94	-0.7427559568724718
1102	95-99	-0.8916256577026687
1102	100-104	-0.7834882513182677
1102	105-109	-1.1413382854786356
1102	110-114	-0.5683687453667119
1102	115-119	-1.1646937259988412
1102	120-124	-1.143018909379009
1102	125-129	-1.0319186656799992
1102	130-134	-1.1512796285000881
1102	135-139	-1.232449823301664
1102	140-144	-1.4711590246241286
1102	145-149	-1.4910498294253038
1102	150-154	-1.3563885364610933
1102	155-159	-1.2775346801182934
1102	160-164	-1.444583711997602
1102	165-169	-1.6919032220909962
1102	170-174	-2.258626772542346
1102	175-179	-2.2597877958332013
1102	180-184	-1.8358541288825556
1102	185-189	-1.8114393346510163
1102	190-194	-2.245744745120927
1102	195-199	-1.987658496724567
1102	200-204	-2.5833728571884755
1102	205-209	-2.276803525878602
1102	210-214	-1.833789294228584
1102	215-219	-2.558084709451645
1102	220-224	-2.796313178860597
1102	225-229	-3.064897136612629
1102	230-234	-2.7158925948317396
1102	235-239	-3.093352237654244
1102	240-244	-3.2992095625925515
1102	245-249	-3.62538541288486
1102	250-254	-3.8269701419803503
1102	255-259	-3.9081273774953367
1102	260-264	-4.039503957780955
1102	265-269	-4.416105370097998
1102	270-274	-4.306474153939536
1102	275-279	-4.411682792975416
1102	280-284	-4.5961034669549115
1102	285-289	-4.832971174250542
1102	290-294	-4.995887624143354
1102	295-299	-5.084790137391078
1102	300-301	-3.5504950059925697
1103	1	-0.32917052685677106
1103	2	-0.2902797872166616
1103	3	0.2589345981239717
1103	4	-0.07331526835066882
1103	5	-0.3680539275030341
1103	6	-0.42459923493827034
1103	7	-0.5924198220319568
1103	8	-0.18438698229925876
1103	9	0.2254743637708856
1103	10-14	-0.3475423376028175
1103	15-19	-0.39265031013894713
1103	20-24	-0.32120980410304867
1103	25-29	-0.4505749097605545
1103	30-34	-0.5444529559766664
1103	35-39	-0.47762434155602307
1103	40-44	-0.4587932900726841
1103	45-49	-0.3480071711166772
1103	50-54	-0.4825257145481956
1103	55-59	-0.34616145335620985
1103	60-64	-0.19237470919042465
1103	65-69	-0.5883290731367694
1103	70-74	-0.661517003994291
1103	75-79	-0.4032874183496489
1103	80-84	-0.5365484874807791
1103	85-89	-0.4651627956317341
1103	90-94	-0.24606195286299481
1103	95-99	-0.8635128456013348
1103	100-104	-0.9613377136838608
1103	105-109	-0.7283676275599227
1103	110-114	-0.8866672314435142
1103	115-119	-0.8416370340058563
1103	120-124	-0.8815442778887537
1103	125-129	-0.9949320634397232
1103	130-134	-0.7858548170204749
1103	135-139	-1.0668514764864518
1103	140-144	-1.0621238081897921
1103	145-149	-1.3525226834238282
1103	150-154	-1.0998087329847728
1103	155-159	-0.99918625863031
1103	160-164	-1.4333829399742548
1103	165-169	-1.3623871152944353
1103	170-174	-1.5560577980394434
1103	175-179	-1.2866350467738066
1103	180-184	-1.4095033138708395
1103	185-189	-1.3099163795643491
1103	190-194	-1.3919408952533274
1103	195-199	-1.2593860347527865
1103	200-204	-1.6884666885649082
1103	205-209	-1.802801706551751
1103	210-214	-1.2155621440712459
1103	215-219	-1.8031945738742756
1103	220-224	-2.2147420289516795
1103	225-229	-2.3813738982318142
1103	230-234	-2.204613172447896
1103	235-239	-2.7622037730096736
1103	240-244	-3.0322060880879
1103	245-249	-3.463219472605097
1103	250-254	-3.495407946688829
1103	255-259	-3.1900681450944717
1103	260-264	-3.6858737613429717
1103	265-269	-3.51050357473995
1103	270-274	-3.7886068487382047
1103	275-279	-4.0420601566661745
1103	280-284	-3.8934507593549057
1103	285-289	-4.010807778576268
1103	290-294	-4.331377574567277
1103	295-299	-4.0806090375936215
1103	300-301	-2.211021924550085
1104	1	-0.25737612744630667
1104	2	-1.2448855351901322
1104	3	-0.4645362935488393
1104	4	-0.42582079524823513
1104	5	-0.7920590859407639
1104	6	-0.5127532511505066
1104	7	-0.4443910821646
1104	8	-0.5919219859838591
1104	9	-0.363692916995511
1104	10-14	-0.5259358527096722
1104	15-19	-0.5164417618707091
1104	20-24	-0.566228227094939
1104	25-29	-0.412623947831527
1104	30-34	-0.5617150061515517
1104	35-39	-0.522978127360183
1104	40-44	-0.724284371413809
1104	45-49	-0.3691325504372287
1104	50-54	-0.694950775609545
1104	55-59	-0.7309923318996283
1104	60-64	-0.4954666342884906
1104	65-69	-0.9461412261972413
1104	70-74	-1.1841004545160807
1104	75-79	-0.8260947605152893
1104	80-84	-0.8468401017210319
1104	85-89	-0.9461542100348161
1104	90-94	-1.190003926070009
1104	95-99	-1.142214011698762
1104	100-104	-1.1545543438807968
1104	105-109	-1.215229252750003
1104	110-114	-1.1463898142421662
1104	115-119	-1.5067011960056327
1104	120-124	-1.3341426172187418
1104	125-129	-1.8520952294768769
1104	130-134	-1.4356354545891108
1104	135-139	-1.4594991275968816
1104	140-144	-1.566470579373071
1104	145-149	-1.9519603118648234
1104	150-154	-1.9767692193069628
1104	155-159	-1.948865940650414
1104	160-164	-2.317147260759853
1104	165-169	-2.204379639594574
1104	170-174	-2.429935323364891
1104	175-179	-2.273472727470388
1104	180-184	-2.304836345988008
1104	185-189	-2.3662475667325396
1104	190-194	-2.6808975980909224
1104	195-199	-2.518456491034801
1104	200-204	-2.9918508934106285
1104	205-209	-3.0835633947711685
1104	210-214	-2.262660813011614
1104	215-219	-2.991924111628201
1104	220-224	-3.0729475355965477
1104	225-229	-3.4416882101024306
1104	230-234	-3.6465428449279322
1104	235-239	-3.616556710805696
1104	240-244	-3.728628245712187
1104	245-249	-4.371528495510756
1104	250-254	-4.307407140695396
1104	255-259	-4.559219080552104
1104	260-264	-5.297819107663319
1104	265-269	-5.01394755315631
1104	270-274	-5.1975690409329545
1104	275-279	-5.118539977817015
1104	280-284	-5.561405333544421
1104	285-289	-5.922808849794652
1104	290-294	-5.27665578383553
1104	295-299	-5.469557312530025
1104	300-301	-4.181997144598398
1201	1	-0.9117239535332615
1201	2	-0.2231464047553473
1201	3	-0.429753684853182
1201	4	0.10678790040393693
1201	5	-0.5311895207233732
1201	6	-0.4366662946287647
1201	7	-1.0096084734689477
1201	8	-0.13974807294037817
1201	9	-0.4528233517781217
1201	10-14	-0.3189793309705422
1201	15-19	-0.38992002274027726
1201	20-24	-0.2857934444862451
1201	25-29	-0.4168903113817777
1201	30-34	-0.5282996766841137
1201	35-39	-0.5909883798758386
1201	40-44	-0.42983971601225335
1201	45-49	-0.5829924327231666
1201	50-54	-0.4461108287707418
1201	55-59	-0.5712904142994759
1201	60-64	-0.7855103024107635
1201	65-69	-0.7582259043821686
1201	70-74	-1.1837093400296617
1201	75-79	-0.9060434979369703
1201	80-84	-0.9360617030675193
1201	85-89	-0.7543664808723101
1201	90-94	-0.611550671891095
1201	95-99	-1.3222560604013793
1201	100-104	-1.1929281276272157
1201	105-109	-1.2408505491654793
1201	110-114	-1.1054889322798687
1201	115-119	-1.2841523918624134
1201	120-124	-1.2086383316273164
1201	125-129	-1.4061433695206453
1201	130-134	-1.2338071694342112
1201	135-139	-1.3390530452136815
1201	140-144	-1.2960043426653236
1201	145-149	-1.4984000520164074
1201	150-154	-1.6725616544429407
1201	155-159	-1.8578230666753797
1201	160-164	-1.7590526638906425
1201	165-169	-1.837772375315847
1201	170-174	-2.157932508608628
1201	175-179	-1.9801568096381388
1201	180-184	-1.7616279094308815
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2102	220-224	2.099691947827761
2102	225-229	2.191145454336791
2102	230-234	2.334100503857009
2102	235-239	2.47103699630021
2102	240-244	2.400390161354995
2102	245-249	2.940893504479188
2102	250-254	2.7401807164222056
2102	255-259	2.820913881723719
2102	260-264	2.99520848973928
2102	265-269	3.2129775645112524
2102	270-274	3.2844891817432504
2102	275-279	3.223588910423942
2102	280-284	3.3887750509636945
2102	285-289	3.5887919870847647
2102	290-294	3.852927591632156
2102	295-299	4.003079215570015
2102	300-301	2.704144402027019
2103	1	1.0453924434252073
2103	2	0.5103396549054011
2103	3	0.4224787191242001
2103	4	0.0638360579395183
2103	5	0.773743214702634
2103	6	0.6171941070089773
2103	7	0.8153574068196079
2103	8	0.5211214922770111
2103	9	0.1925071219984389
2103	10-14	0.40731819291325877
2103	15-19	0.44842858322581236
2103	20-24	0.5996384135757538
2103	25-29	0.49301025403057963
2103	30-34	0.7368611733841277
2103	35-39	0.6898207447098486
2103	40-44	0.5894661915720718
2103	45-49	0.5498209811976764
2103	50-54	0.5938423784821367
2103	55-59	0.6421527520097499
2103	60-64	0.5882116706834069
2103	65-69	1.012947813691536
2103	70-74	0.8798469087517766
2103	75-79	0.6229153634395104
2103	80-84	0.8076295166234999
2103	85-89	0.7709605607564924
2103	90-94	0.8599500559536111
2103	95-99	1.1178183849223373
2103	100-104	0.8910218368779326
2103	105-109	1.1317102469247295
2103	110-114	0.9672952055697692
2103	115-119	0.9801105610468284
2103	120-124	1.0752279540875556
2103	125-129	1.097060862614537
2103	130-134	0.9543606260868778
2103	135-139	1.1714651069498352
2103	140-144	1.1232106041051146
2103	145-149	1.4938796376593402
2103	150-154	1.2769320793943386
2103	155-159	1.4657943990099227
2103	160-164	1.513377214764617
2103	165-169	1.4157366758080805
2103	170-174	1.7672813366270965
2103	175-179	1.5436217859457138
2103	180-184	1.4514912471237302
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2103	220-224	2.13051231250331
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2103	230-234	2.364476672589344
2103	235-239	2.333072221349127
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2103	250-254	2.9327536360009923
2103	255-259	3.1645252116853406
2103	260-264	3.325274907339942
2103	265-269	3.4114541107960186
2103	270-274	3.430439783652762
2103	275-279	3.4971462226769034
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2103	285-289	3.7683364014804326
2103	290-294	4.052114194502977
2103	295-299	3.6546237421293064
2103	300-301	2.7268448107853835
2104	1	0.995408721347097
2104	2	0.31406467354624823
2104	3	0.34210254160033315
2104	4	0.3240570176017812
2104	5	0.37981750503774947
2104	6	0.6355514021434558
2104	7	0.6320522535445932
2104	8	0.29395521593040996
2104	9	0.3880889789417665
2104	10-14	0.37487261893273427
2104	15-19	0.36967749106673864
2104	20-24	0.6591912462669711
2104	25-29	0.48588168290277167
2104	30-34	0.5755398824095863
2104	35-39	0.6341943104691197
2104	40-44	0.42615538502877115
2104	45-49	0.6229067303713407
2104	50-54	0.5964529597575279
2104	55-59	0.6653898926058943
2104	60-64	0.937313227000999
2104	65-69	1.0583827912700094
2104	70-74	1.0409147008910615
2104	75-79	0.8522838677919253
2104	80-84	0.975764573336761
2104	85-89	0.8331193579076484
2104	90-94	0.9633338596993113
2104	95-99	1.2721491683567834
2104	100-104	1.0313681686690828
2104	105-109	1.3725023097978202
2104	110-114	1.1286669546773638
2104	115-119	1.204613365738311
2104	120-124	1.2307205180770744
2104	125-129	1.193341581554872
2104	130-134	1.2400264873099367
2104	135-139	1.3881341064315826
2104	140-144	1.4781090300277917
2104	145-149	1.554582896664492
2104	150-154	1.5223952011782096
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2104	160-164	1.6895435955536442
2104	165-169	1.5514828164598846
2104	170-174	2.013336225134818
2104	175-179	1.6268722147621695
2104	180-184	1.879921778856147
2104	185-189	2.245260204857523
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2104	270-274	3.3878167975237012
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2104	290-294	4.287210228426645
2104	295-299	3.8543549779196873
2104	300-301	2.9304535999437817
2201	1	1.110025383066045
2201	2	0.44730674894948663
2201	3	0.49668028126234987
2201	4	0.2832933096711372
2201	5	0.8138809018118351
2201	6	0.5708352362959133
2201	7	0.9185911591091269
2201	8	0.8990557645774828
2201	9	0.22923421098978736
2201	10-14	0.4889978072209331
2201	15-19	0.5482285790151877
2201	20-24	0.4779705889899759
2201	25-29	0.45207886600136504
2201	30-34	0.609812182879061
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2201	40-44	0.5153572629630503
2201	45-49	0.5853480449253397
2201	50-54	0.4988003775979024
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2201	60-64	-1.216036618200235
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2201	70-74	0.3545868418286524
2201	75-79	0.042367594885277526
2201	80-84	0.13135673386510405
2201	85-89	-0.026811014241218345
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2204	1	1.168710829075433
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2204	3	0.4276176294896459
2204	4	0.2733046420331249
2204	5	0.4481208241042154
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2204	7	1.021650896845891
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2204	9	0.4901051839540145
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2301	1	-3.173331926268986
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2301	4	0.31172438702998306
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2301	6	0.2711463667365166
2301	7	0.606858530558732
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2301	9	0.6089137131810816
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2301	40-44	0.3857422809091986
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2301	60-64	0.30546821787563516
2301	65-69	0.585699322998547
2301	70-74	0.5938941548736061
2301	75-79	0.3550736229284297
2301	80-84	0.19186364454419902
2301	85-89	0.3107292650283924
2301	90-94	0.32960912004269005
2301	95-99	0.6082189685256552
2301	100-104	0.5883371618003679
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2301	140-144	0.7984659074649691
2301	145-149	1.084580623420571
2301	150-154	1.2674304364589624
2301	155-159	1.0170813650559225
2301	160-164	1.1706006539760097
2301	165-169	1.3314759967281269
2301	170-174	1.4029111285219535
2301	175-179	1.3082384343575377
2301	180-184	1.3125098670580009
2301	185-189	1.6838803457986415
2301	190-194	1.6018453561047146
2301	195-199	1.35441649872158
2301	200-204	2.0462916717843136
2301	205-209	2.2531476803107005
2301	210-214	-3.3762793871845282
2301	215-219	1.923771069975718
2301	220-224	2.1586178921892483
2301	225-229	2.3091643692198254
2301	230-234	2.435114828285972
2301	235-239	2.783975439970355
2301	240-244	2.616482591926527
2301	245-249	2.749799691240767
2301	250-254	2.848169339744395
2301	255-259	2.798727129716845
2301	260-264	2.9769222038979812
2301	265-269	3.0940466909838023
2301	270-274	3.2379332804318537
2301	275-279	3.1949669944426127
2301	280-284	3.4559644263804508
2301	285-289	3.7349111246708944
2301	290-294	3.7037509990124526
2301	295-299	3.8841091861990797
2301	300-301	2.5102564763680952
2302	1	1.3267243730934943
2302	2	0.8351075946557813
2302	3	0.8937913146560916
2302	4	0.41174422585295645
2302	5	0.9970271835655637
2302	6	0.8457215746420133
2302	7	1.1195258871159979
2302	8	1.0177354877623372
2302	9	0.6200395395752949
2302	10-14	0.6148787512750147
2302	15-19	0.6740205013543417
2302	20-24	0.7509599169820049
2302	25-29	0.671801414989794
2302	30-34	0.8699346937153365
2302	35-39	0.8344380604028885
2302	40-44	0.70769291721966
2302	45-49	0.8069771423087388
2302	50-54	0.7785084905288855
2302	55-59	0.9180957749588359
2302	60-64	0.9677928650100469
2302	65-69	1.2209891261116361
2302	70-74	1.3583445651444528
2302	75-79	0.9359218768416966
2302	80-84	1.1187509986761341
2302	85-89	1.0731012583601398
2302	90-94	1.016872321237777
2302	95-99	1.3888550507097364
2302	100-104	1.1658116075831018
2302	105-109	1.397256659529969
2302	110-114	1.2415429226746255
2302	115-119	1.4582928249433778
2302	120-124	1.442111867069503
2302	125-129	1.5574315571677566
2302	130-134	1.4057508273604178
2302	135-139	1.494564603158576
2302	140-144	1.5390939271313009
2302	145-149	1.704007446358581
2302	150-154	1.7654736172215912
2302	155-159	1.6848853081008244
2302	160-164	1.8774681238555289
2302	165-169	1.6880094030808088
2302	170-174	2.1595540638998294
2302	175-179	1.8586217859457115
2302	180-184	1.9333094289419073
2302	185-189	1.9488448174770312
2302	190-194	2.2216241523832245
2302	195-199	1.8756229646765128
2302	200-204	2.507558055642594
2302	205-209	2.576591342166008
2302	210-214	3.0782973116810695
2302	215-219	2.8299325233897967
2302	220-224	2.70956183351932
2302	225-229	2.9044363287731265
2302	230-234	2.8487622531429118
2302	235-239	2.703795429969805
2302	240-244	3.207491437704565
2302	245-249	3.396081806071656
2302	250-254	3.482525325498713
2302	255-259	3.67137452675383
2302	260-264	4.071850249805692
2302	265-269	4.0393079920745585
2302	270-274	4.121763984565998
2302	275-279	4.5407038958950885
2302	280-284	4.388374469809687
2302	285-289	4.4497674955945214
2302	290-294	4.796983216372716
2302	295-299	4.94724855860381
2302	300-301	3.0468307590006702
2303	1	0.8120674724320764
2303	2	0.3641293629552038
2303	3	-0.16535721176106222
2303	4	-0.03321412655461131
2303	5	0.4408384513046002
2303	6	0.3724702210294879
2303	7	0.3706742872485975
2303	8	0.29734526850078424
2303	9	0.3189613852240072
2303	10-14	0.2162836669011554
2303	15-19	0.29546256568579565
2303	20-24	0.4134707695605755
2303	25-29	0.2817252759294604
2303	30-34	0.23272495086224865
2303	35-39	0.17335843273183116
2303	40-44	0.31532074996088255
2303	45-49	0.2715991966957745
2303	50-54	0.28496596927883644
2303	55-59	0.40418703389232036
2303	60-64	0.4066392302995183
2303	65-69	0.5038220436064584
2303	70-74	0.6077250362401507
2303	75-79	0.38335918389967105
2303	80-84	0.4034721049475394
2303	85-89	0.5105816998537662
2303	90-94	0.39340422803356745
2303	95-99	0.6327527967240201
2303	100-104	0.5587557442600612
2303	105-109	0.5317860236360197
2303	110-114	0.3390178628064504
2303	115-119	0.6145763352322291
2303	120-124	0.5731468979042624
2303	125-129	0.7605563584901596
2303	130-134	0.733118534567911
2303	135-139	0.8633324841427488
2303	140-144	0.7193098764393575
2303	145-149	0.9149161712192395
2303	150-154	0.90148178202816
2303	155-159	1.2581690804032988
2303	160-164	1.1317671892760899
2303	165-169	1.2090163790985038
2303	170-174	1.3158548942170505
2303	175-179	1.5518004105997427
2303	180-184	1.1007460906408646
2303	185-189	1.3142078530105934
2303	190-194	1.6466137253563637
2303	195-199	1.4116256168843755
2303	200-204	1.5781120249234917
2303	205-209	2.024964574641185
2303	210-214	2.422153416894467
2303	215-219	1.9033960124515552
2303	220-224	1.8563652460778748
2303	225-229	2.043062309664503
2303	230-234	2.1303855426952403
2303	235-239	1.8181494583062872
2303	240-244	2.134331397423896
2303	245-249	2.7068950360117654
2303	250-254	2.501153731220537
2303	255-259	2.4336719147085617
2303	260-264	2.825828911971076
2303	265-269	2.9590093756828217
2303	270-274	2.815606650972363
2303	275-279	2.939777638560411
2303	280-284	3.544435815377426
2303	285-289	3.436105742213247
2303	290-294	3.34303555967362
2303	295-299	3.475109770827114
2303	300-301	1.9017878455118904
2304	1	1.0552356517704702
2304	2	0.6498672657143842
2304	3	0.45652445267379704
2304	4	0.2875053431010315
2304	5	0.5515527960614932
2304	6	0.6372621666724996
2304	7	0.6444926674499527
2304	8	0.5069370951138907
2304	9	0.566130291967383
2304	10-14	0.601041226126803
2304	15-19	0.46982815281734247
2304	20-24	0.4961039755980323
2304	25-29	0.42532163229178366
2304	30-34	0.7203805576668003
2304	35-39	0.6523942904910953
2304	40-44	0.5544875529773563
2304	45-49	0.5679172188340331
2304	50-54	0.7027760044084133
2304	55-59	0.6089593544331748
2304	60-64	0.7989814233575601
2304	65-69	1.0181180158562988
2304	70-74	1.078432709512942
2304	75-79	0.790187358266131
2304	80-84	0.9584929838499079
2304	85-89	0.9662101658620372
2304	90-94	0.9375338318867747
2304	95-99	1.1679122054865303
2304	100-104	1.04362682532323
2304	105-109	1.1226501229709918
2304	110-114	0.9271124696423527
2304	115-119	1.1218331346842945
2304	120-124	1.247293514196432
2304	125-129	1.2914692857343688
2304	130-134	1.205721879611115
2304	135-139	1.3744314435117424
2304	140-144	1.2701693360182382
2304	145-149	1.2827019028645807
2304	150-154	1.5140919762350897
2304	155-159	1.5880005025990087
2304	160-164	1.7656241522243263
2304	165-169	1.3090076837599405
2304	170-174	1.8420395571300041
2304	175-179	1.741336133209824
2304	180-184	1.6428096443662916
2304	185-189	1.8448533372906795
2304	190-194	2.0463734209708058
2304	195-199	1.712795988243652
2304	200-204	2.3907868500806124
2304	205-209	2.309557259967125
2304	210-214	2.8426756381231186
2304	215-219	2.5735225437937714
2304	220-224	2.482950904917441
2304	225-229	2.5667913896705485
2304	230-234	2.728791903774063
2304	235-239	2.825453917462969
2304	240-244	2.9414224729336276
2304	245-249	3.2840684308234387
2304	250-254	3.586688060452456
2304	255-259	3.5944648918492064
2304	260-264	3.87828208281287
2304	265-269	3.9632805948142824
2304	270-274	3.658926411180481
2304	275-279	3.6115975684325967
2304	280-284	3.9849953342603506
2304	285-289	4.146372807219283
2304	290-294	4.084220713784468
2304	295-299	4.238372421793706
2304	300-301	2.588040109813857
2401	1	-0.13397975388420846
2401	2	0.2166176818112362
2401	3	0.0973666115008811
2401	4	0.11988986959871539
2401	5	0.3544607034918741
2401	6	0.03647661821974424
2401	7	0.3534739987478588
2401	8	0.4471304608868749
2401	9	0.44811250316436
2401	10-14	0.3503675202675254
2401	15-19	0.3790226276657691
2401	20-24	0.5800394817472707
2401	25-29	0.33855668422154395
2401	30-34	0.5606451205801761
2401	35-39	0.5616300861720305
2401	40-44	0.4604582810566171
2401	45-49	0.5702855350345359
2401	50-54	0.5054280085521725
2401	55-59	0.6525835475959809
2401	60-64	0.6765121694993539
2401	65-69	0.9723603193598969
2401	70-74	1.0465300346650146
2401	75-79	0.7854709133966793
2401	80-84	0.7390428284590413
2401	85-89	0.6098067339816708
2401	90-94	0.7780172701421506
2401	95-99	1.0468061412583154
2401	100-104	0.993943185192542
2401	105-109	1.1479264631409514
2401	110-114	1.0835114217859854
2401	115-119	1.0900204709567376
2401	120-124	1.353676492919849
2401	125-129	1.2718092001168237
2401	130-134	1.2639386069767689
2401	135-139	1.2906668278322293
2401	140-144	1.4289419384078705
2401	145-149	1.6593423731220796
2401	150-154	1.5923170097792756
2401	155-159	1.427403735619258
2401	160-164	1.7349456013330098
2401	165-169	1.7319774620488637
2401	170-174	1.9524574218031852
2401	175-179	1.8619223592462824
2401	180-184	1.8042758499083256
2401	185-189	1.9757038560558868
2401	190-194	2.0043982704648577
2401	195-199	1.7318451321743105
2401	200-204	2.3757727828108166
2401	205-209	2.4763594381362246
2401	210-214	2.354718687987784
2401	215-219	2.399022955677413
2401	220-224	2.3120330769054185
2401	225-229	2.725830118270089
2401	230-234	2.6845824272509056
2401	235-239	2.929319270835304
2401	240-244	3.525632914588723
2401	245-249	3.235639183036536
2401	250-254	3.586009250996309
2401	255-259	3.640329452179273
2401	260-264	3.8652905746972124
2401	265-269	3.887967104250496
2401	270-274	3.7973254631089475
2401	275-279	4.1554975899796815
2401	280-284	4.36193239315465
2401	285-289	4.636969682405315
2401	290-294	4.710187950321089
2401	295-299	4.8722791224583055
2401	300-301	3.315293417623515
2402	1	0.7369434912678265
2402	2	1.0719562117216483
2402	3	0.4271084880871925
2402	4	0.2956605781590227
2402	5	0.9878261392318848
2402	6	0.8176134446961996
2402	7	0.5194986172809806
2402	8	0.6598239531271233
2402	9	0.49198746429035367
2402	10-14	0.48684889585564406
2402	15-19	0.5597356527776753
2402	20-24	0.5672480884385678
2402	25-29	0.5228353962342069
2402	30-34	0.8639666398220669
2402	35-39	0.6742611094734414
2402	40-44	0.5165546036064583
2402	45-49	0.6269871412482431
2402	50-54	0.6796240219247167
2402	55-59	0.7096209756431406
2402	60-64	0.7177559168286152
2402	65-69	1.1504454310910397
2402	70-74	1.1098827229992345
2402	75-79	0.879879077687967
2402	80-84	1.0110323717312895
2402	85-89	0.6705432294696934
2402	90-94	0.8726124489873754
2402	95-99	1.0976851599346276
2402	100-104	1.0491768277031568
2402	105-109	1.1704550319782392
2402	110-114	0.9428136770613946
2402	115-119	1.007228282795424
2402	120-124	1.1975243676402023
2402	125-129	1.1309602975229573
2402	130-134	1.273470622213484
2402	135-139	1.2214969868503083
2402	140-144	1.2012528399134048
2402	145-149	1.5114736990788984
2402	150-154	1.4789546541350411
2402	155-159	1.3763547000064662
2402	160-164	1.5924793095378575
2402	165-169	1.563206677841837
2402	170-174	1.6812227643269146
2402	175-179	1.7422499963003766
2402	180-184	1.4751561472460288
2402	185-189	1.8222645997995812
2402	190-194	1.815111905448152
2402	195-199	1.7746682924132067
2402	200-204	2.2096419541862637
2402	205-209	2.1666944877215393
2402	210-214	2.649268108435699
2402	215-219	2.4664731801428808
2402	220-224	2.0770722632498106
2402	225-229	2.6390794144376883
2402	230-234	2.6822510299850713
2402	235-239	2.980336086722893
2402	240-244	3.475367867793384
2402	245-249	3.6131287206630383
2402	250-254	3.529852809978731
2402	255-259	3.877634852032166
2402	260-264	3.888415844182589
2402	265-269	4.292007186574587
2402	270-274	4.434790509628847
2402	275-279	4.422781320402866
2402	280-284	4.365241062429703
2402	285-289	4.371191659484442
2402	290-294	4.725466494171062
2402	295-299	4.950945190233963
2402	300-301	3.576621548843292
2404	1	0.8974103806449385
2404	2	0.2771557984031645
2404	3	0.5727029343708381
2404	4	0.2387239503162064
2404	5	0.6257611519223687
2404	6	0.5544138379506833
2404	7	0.8355367790169197
2404	8	0.646682030393606
2404	9	0.4099959112361091
2404	10-14	0.5772733498639369
2404	15-19	0.5015257767563099
2404	20-24	0.6726237514101925
2404	25-29	0.536309493210311
2404	30-34	0.8069372554116399
2404	35-39	0.6811806479697822
2404	40-44	0.6182110900453921
2404	45-49	0.6381507035738565
2404	50-54	0.6726190197881294
2404	55-59	0.7204487161353157
2404	60-64	0.852691944780247
2404	65-69	1.1903378474497792
2404	70-74	1.3521480121931013
2404	75-79	0.9203023740708431
2404	80-84	0.9763461992455547
2404	85-89	1.0008179699367332
2404	90-94	1.0157700791309097
2404	95-99	1.4243342311459557
2404	100-104	1.2298982413723252
2404	105-109	1.3653573772010752
2404	110-114	1.0850905289830663
2404	115-119	1.2417746970929855
2404	120-124	1.4149499011595417
2404	125-129	1.3810530507089958
2404	130-134	1.4546957686328064
2404	135-139	1.5866077125371625
2404	140-144	1.4671613938183334
2404	145-149	1.589007318966793
2404	150-154	1.742075082458694
2404	155-159	1.682970088488986
2404	160-164	1.9083670002600215
2404	165-169	1.7849808024679383
2404	170-174	1.997669487801769
2404	175-179	1.9477944110733922
2404	180-184	1.8121551899224961
2404	185-189	2.0600980255062353
2404	190-194	2.2501680852234323
2404	195-199	1.9728705396042123
2404	200-204	2.404698133961549
2404	205-209	2.5284922405305217
2404	210-214	3.0536308981917557
2404	215-219	2.707598800144904
2404	220-224	2.5791979844766466
2404	225-229	2.669744461507232
2404	230-234	2.7708014905579965
2404	235-239	3.1207413927314676
2404	240-244	3.5590414924668288
2404	245-249	3.7819145780703955
2404	250-254	3.726415571538066
2404	255-259	3.865611619222161
2404	260-264	4.085140834648389
2404	265-269	4.051591303713231
2404	270-274	4.340764377136097
2404	275-279	4.240565463282845
2404	280-284	4.224013635908161
2404	285-289	4.332893180009481
2404	290-294	4.541397357160751
2404	295-299	4.645800256811718
2404	300-301	3.1650136997485134
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	15.0
16	384.0
17	873.0
18	909.0
19	874.0
20	997.0
21	1062.0
22	1262.0
23	1624.0
24	2104.0
25	2849.0
26	4247.0
27	6537.0
28	9764.0
29	13500.0
30	16643.0
31	18449.0
32	25999.0
33	31156.0
34	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	99.3912157020403	0.16296466466610193	0.05025342082214596	0.39556621247146323
2	0.4709463437046822	98.89083521185407	0.137120048243284	0.5010983961979697
3	0.4285898890117306	0.4616135655519979	0.17516906686576594	98.9346274785705
4	0.17373325484227603	0.30798167903858026	99.18804830071647	0.3302367654026735
5	0.25629244619294445	98.9777018392752	0.44151219722313956	0.3244935173087139
6	0.25485663416945453	0.6461154105704481	0.1830660329949603	98.91596192226514
7	0.39556621247146323	31.872155297428463	37.493359369391364	30.23891912070872
8	34.547073097190115	33.54415839878243	0.47668959179864173	31.43207891222881
9	99.24332706362084	0.16655419472482663	0.30439214897985556	0.285726592674487
10-14	39.94859792955906	19.95003374158255	39.87206914870705	0.2292991801513346
15-19	0.2511397494346125	39.876512187241985	39.74311663136734	20.12923143195606
20-24	19.305532218014285	0.9933893721555127	39.23952647051643	40.46155193931378
25-29	20.082965618679903	0.30824829931972786	48.42399797756941	31.18478810443096
30-34	0.39029785889231255	30.41881244298879	1.007994139067853	68.18289555905105
35-39	19.419554026931934	1.533651988144547	50.672005355929386	28.37478862899414
40-44	36.07643868609153	15.597130531501211	24.64799864344037	23.678432138966887
45-49	2.5579881069939328	19.997038966340522	20.429119897427494	57.01585302923805
50-54	38.45328870947337	1.7700031052686058	22.855351987946957	36.92135619731107
55-59	31.915900146521935	26.07659114340313	39.51706714558299	2.49044156449194
60-64	21.665352586053096	26.443810104800324	11.146616865888962	40.74422044325762
65-69	35.472589519776456	38.261602281565985	3.4566300809495	22.80917811770806
70-74	43.12615205463855	16.6220136654037	2.8064375263918695	37.44539675356587
75-79	0.7716832322486913	0.27298618638009364	40.227699882388215	58.727630698983
80-84	20.410625582005473	21.561518879320534	1.3697129397654968	56.6581425989085
85-89	57.85659191338134	1.9774658852533464	37.789119324120726	2.3768228772445807
90-94	0.3970988998312978	0.30063587732325925	71.85990512306604	27.44236009977939
95-99	3.954724034683882	37.77987388728315	18.096684493212646	40.16871758482032
100-104	0.33193727251472793	17.046710790270485	58.739331103099715	23.88202083411507
105-109	20.23343270223255	39.24628810243899	1.0567268895128517	39.46355230581561
110-114	0.28688915134521703	20.12743249369768	48.19939878891484	31.38627956604226
115-119	19.95205291509611	0.8054243750270785	19.80821166038444	59.43431104949237
120-124	0.5311925757278074	49.918811469252375	6.231869820980735	43.31812613403908
125-129	50.85708671428901	35.2389497653559	12.868148507755969	1.0358150125991168
130-134	3.0396964060944223	16.810529829748678	41.06148036712203	39.088293397034874
135-139	3.1452377648256085	4.044083634008697	34.96695462114904	57.84372398001665
140-144	0.732074795111597	3.286745440979994	49.38576857009006	46.59541119381836
145-149	21.118412202964247	23.228850417936485	17.443695371384187	38.209042007715084
150-154	0.7948601072258036	36.55097566799071	21.898518952631086	40.7556452721524
155-159	24.155070445775426	43.030109164174696	21.21999866837655	11.594821721673329
160-164	22.576325268412457	31.569735120047255	21.744594109403412	24.109345502136875
165-169	13.762526624280186	14.77667459663582	42.72959867019775	28.731200108886245
170-174	25.341225747771023	32.67902765145151	19.176568392878867	22.803178207898593
175-179	14.549690048372446	9.416618738491806	41.802833388142886	34.23085782499287
180-184	42.853147866405855	5.45519090052887	33.280301383757156	18.41135984930812
185-189	28.301435545402892	31.302253040967543	17.658194088521434	22.738117325108128
190-194	45.414512843191986	20.277091051452516	7.26338149681367	27.045014608541827
195-199	15.498405433274018	5.8101979757196585	61.46253457069971	17.228862020306615
200-204	2.3027953082123696	36.19041123521482	18.56181588375883	42.94497757281398
205-209	17.817598327149692	35.63693920670302	9.879110426997945	36.666352039149345
210-214	6.76189616145885	27.335671385703144	56.82414717590611	9.078285276931892
215-219	5.401236364483044	22.349732819539682	19.414531357323305	52.83449945865397
220-224	29.357287928310928	7.632032961833284	20.994387461691332	42.01629164816446
225-229	21.523474551205744	7.945199092576701	15.65887994125352	54.87244641496404
230-234	12.705186843555143	3.6361542789345878	59.1615870989182	24.497071778592066
235-239	17.392661208704354	20.01878301245687	0.48240842552273067	62.10614735331605
240-244	8.641587915496041	50.691137300689604	21.84784908964863	18.819425694165723
245-249	3.8761988074357823	29.869746751701005	26.801442292947865	39.45261214791535
250-254	33.00411402348574	43.90269567187192	9.8039444935659	13.28924581107644
255-259	20.484934300120386	19.466999395879988	20.581183928944956	39.46688237505467
260-264	20.17605757518973	15.792917876885598	18.020647896561417	46.01037665136325
265-269	3.6924019446191005	18.14499049528575	20.53106216830599	57.63154539178916
270-274	16.36473545132689	22.095730804613552	59.328273350578165	2.2112603934813966
275-279	23.888553995442184	16.716018525325296	0.9592001764316694	58.436227302800845
280-284	56.15481027964293	0.5219647909416133	19.93348778228295	23.389737147132507
285-289	40.94217114132567	3.7302755595413335	19.70349545552432	35.624057843608675
290-294	25.05616764359171	35.25257321021563	17.75341258184798	21.937846564344675
295-299	35.19722499873944	19.321050087052466	40.5390134449345	4.942711469273594
300-301	17.023819631765605	1.5779607991692195	0.5695288977133937	80.82869067135178
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.25
11	0.5
12	0.0
13	0.0
14	0.5
15	1.0
16	1.0
17	1.0
18	1.0
19	1.0
20	1.75
21	2.25
22	3.0
23	5.5
24	7.0
25	7.25
26	6.333333333333333
27	5.666666666666666
28	7.833333333333333
29	9.583333333333332
30	9.166666666666666
31	9.5
32	10.083333333333334
33	10.083333333333332
34	11.416666666666666
35	13.833333333333336
36	15.16666666666667
37	15.833333333333334
38	16.5
39	17.916666666666668
40	22.999999999999993
41	24.41666666666666
42	33.91666666666666
43	51.58333333333337
44	78.83333333333337
45	129.16666666666637
46	175.6666666666667
47	1329.9166666666667
48	1769.4999999999984
49	535.2499999999992
50	575.1666666666669
51	1411.4166666666652
52	9527.749999999995
53	16587.500000000022
54	5828.666666666664
55	3583.0000000000045
56	3883.9166666666747
57	1878.6666666666645
58	192.9166666666667
59	59.500000000000014
60	47.91666666666668
61	24.083333333333332
62	11.5
63	8.25
64	4.25
65	0.75
66	0.5
67	0.3333333333333333
68	0.0
69	0.9166666666666665
70	0.6666666666666666
71	0.75
72	0.75
73	0.25
74	0.0
75	0.0
76	0.25
77	0.25
78	0.0
79	0.0
80	0.0
81	0.3333333333333333
82	0.0
83	0.25
84	0.0
85	0.3333333333333333
86	0.6666666666666666
87	0.3333333333333333
88	0.3333333333333333
89	0.25
90	0.5
91	0.75
92	2.1666666666666665
93	2.4166666666666665
94	2.8333333333333335
95	3.1666666666666665
96	3.333333333333333
97	6.583333333333334
98	13.5
99	26.0
100	77.33333333333336
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	1.4363833017568404E-4
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	1.4413188644136932E-4
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	1.4459287706569E-4
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	1.4479533179850282E-4
170-174	0.0
175-179	0.0
180-184	0.0
185-189	1.4494581200818075E-4
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	1.8006126095217605
215-219	2.9104540599378913E-4
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	1.4672265603220857E-4
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
10-19	28.0
20-29	35.0
30-39	28.0
40-49	80.0
50-59	110.0
60-69	230.0
70-79	32.0
80-89	38.0
90-99	107.0
100-109	77.0
110-119	67.0
120-129	47.0
130-139	77.0
140-149	107.0
150-159	79.0
160-169	47.0
170-179	67.0
180-189	91.0
190-199	87.0
200-209	187.0
210-219	270.0
220-229	173.0
230-239	156.0
240-249	186.0
250-259	215.0
260-269	293.0
270-279	475.0
280-289	526.0
290-299	1228.0
300-302	134151.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.914238947836948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.6142385439317	6.205579565523282
2	10.944672626238223	1.9512685399227534
3	5.20254489812354	1.3913018507616983
4	2.754288475477168	0.9820954240670812
5	2.182491745188049	0.9727626459143969
6	1.6751228154948858	0.895946702657688
7	1.224128211323186	0.7638519964966186
8	0.9019892083434002	0.6432437865234684
9	0.6442780059595716	0.5168923284563585
>10	3.6321172585970847	5.971542205694431
>50	0.4348876540227108	2.543540999612331
>100	0.41072722879922685	7.421712349419214
>500	0.17717645163888218	11.539621232788203
>1k	0.1288556011919143	15.160021250017946
>5k	0.0724812756704518	43.04061912214453
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7718	5.5407985986474655	No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7499	5.383577182075323	No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7492	5.378551839993108	No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6598	4.736743865493129	No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6576	4.720949933234741	No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6283	4.510603471793473	No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6272	4.502706505664278	No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6014	4.317486754634083	No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	5501	3.9492009706089277	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	1696	1.2175685959194222	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	1683	1.208235817766738	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	1656	1.1888523554496246	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	1437	1.0316309388774823	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	1427	1.0244518787600327	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	1401	1.0057863224546642	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	1387	0.9957356382902351	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	1275	0.9153301649748015	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	1233	0.8851781124815139	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	1227	0.8808706764110443	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	1208	0.8672304621878903	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	1203	0.8636409321291656	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	1120	0.8040547331543354	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	1067	0.7660057145318535	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	1056	0.7581087484026592	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	1041	0.747340158226485	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	985	0.7071374215687682	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	965	0.6927793013338693	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	942	0.6762674630637358	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	935	0.6712421209815211	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	874	0.6274498542650797	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	849	0.609502203971456	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	843	0.6051947679009864	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	830	0.5958619897483021	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	829	0.5951440837365573	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	767	0.5506339110083708	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	701	0.5032521142332046	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	692	0.49679096012750007	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	688	0.4939193360805203	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	685	0.4917656180452855	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	629	0.4515628813875687	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	595	0.4271540769882407	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	577	0.41423176877683177	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	565	0.4056168966358924	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	564	0.40489899062414747	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	538	0.386233434318779	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	511	0.3668499720016655	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	510	0.3661320659899206	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	481	0.3453127916493173	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	470	0.3374158255201229	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	453	0.3252114233204589	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	451	0.323775611296969	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	410	0.29434146481542633	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	404	0.2900340287449567	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	395	0.28357287463925224	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG	246	0.1766048788892558	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG	244	0.17516906686576594	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG	235	0.16870791276006145	No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	231	0.16583628871308168	No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	229	0.1644004766895918	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG	229	0.1644004766895918	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG	220	0.15793932258388732	No Hit
GACTACTAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	214	0.15363188651341766	No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	213	0.1529139805016727	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG	207	0.14860654443120308	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG	206	0.14788863841945812	No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	200	0.14358120234898847	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG	197	0.14142748431375365	No Hit
GACTACCGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	184	0.13209470616106941	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG	181	0.12994098812583457	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	172	0.12347983402013007	No Hit
GACTACACGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	170	0.1220440219966402	No Hit
GACTACCAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	167	0.11989030396140536	No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG	166	0.11917239794966043	No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG	162	0.11630077390268065	No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG	162	0.11630077390268065	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA	158	0.1134291498557009	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	155	0.11127543182046606	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	151	0.1084038077734863	No Hit
GACTACCGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG	150	0.10768590176174135	No Hit
GACTACAAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG	150	0.10768590176174135	No Hit
GACTACAGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG	148	0.10625008973825148	No Hit
GACTACACGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG	143	0.10266055967952675	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	142	0.10194265366778182	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	141	0.10122474765603687	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0011486496187919078	0.0	0.0	0.0
175-179	0.0	0.0014358120234898848	0.0	0.0	0.0
180-184	0.0	0.0014358120234898848	0.0	0.0	0.0
185-189	0.0	0.0014358120234898848	0.0	0.0	0.0
190-194	0.0	0.0014358120234898848	0.0	0.0	0.0
195-199	0.0	0.0014358120234898848	0.0	0.0	0.0
200-204	0.0	0.0014358120234898848	0.0	0.0	0.0
205-209	0.0	0.0014358120234898848	0.0	0.0	0.0
210-214	0.0	0.0014358120234898848	0.0	0.0	0.0
215-219	0.0	0.0014358120234898848	0.0	0.0	0.0
220-224	0.0	0.0014358120234898848	0.0	0.0	0.0
225-229	0.0	0.0014358120234898848	0.0	0.0	0.0
230-234	0.0	0.0014358120234898848	0.0	0.0	0.0
235-239	0.0	0.0014358120234898848	0.0	0.0	0.0
240-244	0.0	0.0014358120234898848	0.0	0.0	0.0
245-249	0.0	0.0014358120234898848	0.0	0.0	0.0
250-254	0.0	0.0014358120234898848	0.0	0.0	0.0
255-259	0.0	0.0014358120234898848	0.0	0.0	0.0
260-264	0.0	0.0014358120234898848	0.0	0.0	0.0
265-269	0.0	0.0014358120234898848	0.0	0.0	0.0
270-274	0.0	0.0014358120234898848	0.0	0.0	0.0
275-279	0.0	0.0014358120234898848	0.0	0.0	0.0
280-284	0.0	0.0014358120234898848	0.0	0.0	0.0
285-289	0.0	0.0014358120234898848	0.0	0.0	0.0
290	0.0	0.0014358120234898848	0.0	0.0	0.0
>>END_MODULE
