##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	ICFS1_cleaned_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	139294
Sequences flagged as poor quality	0
Sequence length	16-301
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20581647450716	34.0	34.0	34.0	20.0	34.0
2	33.10164113314285	34.0	34.0	34.0	34.0	34.0
3	33.60266773873965	34.0	34.0	34.0	34.0	34.0
4	33.42640745473603	34.0	34.0	34.0	34.0	34.0
5	33.24366447944635	34.0	34.0	34.0	34.0	34.0
6	33.11196462159174	34.0	34.0	34.0	34.0	34.0
7	33.525033382629545	34.0	34.0	34.0	34.0	34.0
8	33.47645268281477	34.0	34.0	34.0	34.0	34.0
9	33.3725429666748	34.0	34.0	34.0	34.0	34.0
10-14	33.29300759544561	34.0	34.0	34.0	34.0	34.0
15-19	33.46987160838348	34.0	34.0	34.0	34.0	34.0
20-24	33.66072379803724	34.0	34.0	34.0	34.0	34.0
25-29	33.54594729768756	34.0	34.0	34.0	34.0	34.0
30-34	33.49356978547088	34.0	34.0	34.0	34.0	34.0
35-39	33.574886242662586	34.0	34.0	34.0	34.0	34.0
40-44	33.416851547473605	34.0	34.0	34.0	34.0	34.0
45-49	33.57204292781235	34.0	34.0	34.0	34.0	34.0
50-54	33.51734569935611	34.0	34.0	34.0	34.0	34.0
55-59	33.37753168941664	34.0	34.0	34.0	34.0	34.0
60-64	33.316743624669684	34.0	34.0	34.0	34.0	34.0
65-69	33.47925473590391	34.0	34.0	34.0	34.0	34.0
70-74	33.521143776531176	34.0	34.0	34.0	34.0	34.0
75-79	33.3970587937195	34.0	34.0	34.0	34.0	34.0
80-84	33.599904850090425	34.0	34.0	34.0	34.0	34.0
85-89	33.574715421381576	34.0	34.0	34.0	34.0	34.0
90-94	33.20622548436518	34.0	34.0	34.0	34.0	34.0
95-99	33.35743272050505	34.0	34.0	34.0	34.0	34.0
100-104	33.287301054844185	34.0	34.0	34.0	34.0	34.0
105-109	33.40146699781009	34.0	34.0	34.0	34.0	34.0
110-114	33.25808453553164	34.0	34.0	34.0	34.0	34.0
115-119	33.24259309367581	34.0	34.0	34.0	34.0	34.0
120-124	33.28146072069139	34.0	34.0	34.0	34.0	34.0
125-129	33.28526971338331	34.0	34.0	34.0	34.0	34.0
130-134	33.20465915546494	34.0	34.0	34.0	34.0	34.0
135-139	33.2824203142815	34.0	34.0	34.0	34.0	34.0
140-144	33.312321583480475	34.0	34.0	34.0	34.0	34.0
145-149	33.296524046346676	34.0	34.0	34.0	34.0	34.0
150-154	33.1271357842059	34.0	34.0	34.0	34.0	34.0
155-159	33.184883975559515	34.0	34.0	34.0	34.0	34.0
160-164	33.318959258739596	34.0	34.0	34.0	34.0	34.0
165-169	33.32893185828016	34.0	34.0	34.0	34.0	34.0
170-174	32.82575819779375	34.0	34.0	34.0	31.2	34.0
175-179	33.20124772872915	34.0	34.0	34.0	34.0	34.0
180-184	33.12007618527504	34.0	34.0	34.0	34.0	34.0
185-189	33.161670465813344	34.0	34.0	34.0	34.0	34.0
190-194	33.20048378590778	34.0	34.0	34.0	34.0	34.0
195-199	32.74070737961048	34.0	34.0	34.0	31.2	34.0
200-204	33.03985650952345	34.0	34.0	34.0	34.0	34.0
205-209	32.719885410322156	34.0	34.0	34.0	31.2	34.0
210-214	32.68086455153595	34.0	34.0	34.0	34.0	34.0
215-219	33.038935218569485	34.0	34.0	34.0	34.0	34.0
220-224	33.07898099339961	34.0	34.0	34.0	34.0	34.0
225-229	33.16741873998207	34.0	34.0	34.0	34.0	34.0
230-234	32.86099518540743	34.0	34.0	34.0	31.2	34.0
235-239	32.996588173715445	34.0	34.0	34.0	34.0	34.0
240-244	32.81096779798914	34.0	34.0	34.0	34.0	34.0
245-249	32.87642019570046	34.0	34.0	34.0	34.0	34.0
250-254	32.59364243439082	34.0	34.0	34.0	31.2	34.0
255-259	32.575983099330074	34.0	34.0	34.0	31.2	34.0
260-264	32.723874946844745	34.0	34.0	34.0	34.0	34.0
265-269	32.76528672752663	34.0	34.0	34.0	34.0	34.0
270-274	32.524966277593066	34.0	34.0	34.0	34.0	34.0
275-279	32.362976425790734	34.0	34.0	34.0	31.2	34.0
280-284	32.17087143802993	34.0	34.0	34.0	25.6	34.0
285-289	31.995835648671495	34.0	34.0	34.0	25.6	34.0
290-294	31.964550323249263	34.0	34.0	34.0	22.8	34.0
295-299	31.748529116713616	34.0	34.0	34.0	20.0	34.0
300-301	30.915951253059845	34.0	34.0	34.0	14.5	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.07808796069662804
1101	2	0.05470524811876487
1101	3	0.04535612521533494
1101	4	-0.20591336622981515
1101	5	-0.012980166283355743
1101	6	-0.17403605584417647
1101	7	-0.2107925190624016
1101	8	0.21735983684382632
1101	9	-0.10889853594302679
1101	10-14	0.047275883116704165
1101	15-19	-0.09972799483339401
1101	20-24	0.035237913835757695
1101	25-29	0.020167561817537205
1101	30-34	-0.04646301501839645
1101	35-39	0.016210766669068732
1101	40-44	-0.012329825653907278
1101	45-49	0.045804435243574915
1101	50-54	0.07056301859632441
1101	55-59	-0.07963640522179816
1101	60-64	0.03689281085735985
1101	65-69	0.030943002352117333
1101	70-74	-0.055690704119001566
1101	75-79	0.07748608703469273
1101	80-84	0.06180515775439943
1101	85-89	-0.06307670047804237
1101	90-94	-0.2152197966598095
1101	95-99	-0.15329219563515295
1101	100-104	-0.03628862948989564
1101	105-109	-0.06139788800542334
1101	110-114	-0.20370864743497208
1101	115-119	0.002112278479629026
1101	120-124	0.014824574766436172
1101	125-129	-0.028267271709140118
1101	130-134	0.04410172938241175
1101	135-139	0.010912858647593282
1101	140-144	0.049064260597617704
1101	145-149	0.01886237674951019
1101	150-154	-0.03172532578991394
1101	155-159	-0.12335341127194965
1101	160-164	-0.08436920793921132
1101	165-169	-0.1334179868786407
1101	170-174	-0.2844746880437441
1101	175-179	-0.023662837601150954
1101	180-184	-0.01122142769808221
1101	185-189	-0.06252293913573226
1101	190-194	-0.08218058432478159
1101	195-199	-0.05394045607950915
1101	200-204	0.07258895105034213
1101	205-209	-0.07667425728438815
1101	210-214	-0.04523796440236083
1101	215-219	-0.2143380990543875
1101	220-224	-0.2257958350890945
1101	225-229	-0.24335548535584905
1101	230-234	-0.22651151586588725
1101	235-239	-0.4662243994805664
1101	240-244	-0.2145284140068071
1101	245-249	-0.3467877113672273
1101	250-254	-0.3490391237749577
1101	255-259	-0.41096064065337856
1101	260-264	-0.2487890538239128
1101	265-269	-0.3750385838150052
1101	270-274	-0.35479870470783936
1101	275-279	-0.35626299167423525
1101	280-284	-0.4690067783207752
1101	285-289	-0.7231793201744701
1101	290-294	-0.6406533475339131
1101	295-299	-0.8736980467167186
1101	300-301	-0.6125986760823707
1102	1	0.16365454465031348
1102	2	0.08354450921608958
1102	3	0.049615843991183795
1102	4	0.07706154416445088
1102	5	0.0078221496802513
1102	6	-0.09027537208348946
1102	7	0.12034892014354881
1102	8	-0.08330186704368714
1102	9	-0.0359732533403232
1102	10-14	0.04834563773484746
1102	15-19	-0.00613549414411807
1102	20-24	0.009197108762698747
1102	25-29	0.08200103300585226
1102	30-34	0.10546971175303099
1102	35-39	0.052022732481027845
1102	40-44	-0.06735877519898992
1102	45-49	-0.05595183715786334
1102	50-54	0.058492436848325724
1102	55-59	-0.004011922177959093
1102	60-64	-0.08847250483053415
1102	65-69	-0.14595801777471706
1102	70-74	-0.047000326396364756
1102	75-79	-0.2540330748070545
1102	80-84	-0.01676390758886015
1102	85-89	-0.0690844203567309
1102	90-94	-0.11631712227325153
1102	95-99	-0.2107363643696445
1102	100-104	-0.22848328071680868
1102	105-109	-0.24374417970655315
1102	110-114	-0.1533805525907539
1102	115-119	-0.15955300963727126
1102	120-124	-0.1520451372258833
1102	125-129	-0.16475227090326428
1102	130-134	-0.5465915542168602
1102	135-139	-0.3116281834651815
1102	140-144	-0.34968778287364444
1102	145-149	-0.3637739889800429
1102	150-154	-0.12760338524053338
1102	155-159	-0.34106057896322994
1102	160-164	-0.3035380044209859
1102	165-169	-0.2740189401148072
1102	170-174	-0.452849351302703
1102	175-179	-0.22784053246236624
1102	180-184	-0.24187840916452785
1102	185-189	-0.1437343539616336
1102	190-194	-0.2872568015809591
1102	195-199	-0.5854466718557418
1102	200-204	0.10029725355144592
1102	205-209	-0.15543887175826399
1102	210-214	-0.24885088433723723
1102	215-219	-0.21753848384053498
1102	220-224	-0.4567859194460553
1102	225-229	-0.2949353284815004
1102	230-234	-0.36283865808876214
1102	235-239	-0.3802098960026967
1102	240-244	-0.6183633259499715
1102	245-249	-0.3071658377546882
1102	250-254	-0.5146340623571675
1102	255-259	-0.5994485050902618
1102	260-264	-0.5798161368489261
1102	265-269	-0.6319036866713006
1102	270-274	-0.6759822920986913
1102	275-279	-0.7914080264529915
1102	280-284	-1.0692879681979335
1102	285-289	-1.222827257470751
1102	290-294	-1.1749547407615069
1102	295-299	-1.1108246798123496
1102	300-301	-1.2519396057438037
1103	1	-0.21015628093744354
1103	2	-0.14136892253167588
1103	3	-0.16998503080378669
1103	4	0.0784557486477766
1103	5	-0.3008984626762228
1103	6	-0.01923017434337737
1103	7	-0.24746135869734331
1103	8	-0.19985007189151105
1103	9	-0.13265632241267156
1103	10-14	-0.17319709964441898
1103	15-19	-0.10585938698173436
1103	20-24	0.007297163437385734
1103	25-29	0.05165749373120576
1103	30-34	0.010885239363751964
1103	35-39	-0.01995466864890716
1103	40-44	-0.09710818288989742
1103	45-49	-0.12434622389013583
1103	50-54	-0.034485511028471194
1103	55-59	-0.058210938033617765
1103	60-64	-0.11919512192547899
1103	65-69	-0.12129153335700238
1103	70-74	0.007611483335722369
1103	75-79	-0.1202359178907102
1103	80-84	-0.024114007825779993
1103	85-89	-0.05490271815151715
1103	90-94	0.07972443048780775
1103	95-99	-0.1548132734274148
1103	100-104	3.512372347174164E-4
1103	105-109	-0.08712945399122418
1103	110-114	-0.03880441489875608
1103	115-119	-0.25144534577863453
1103	120-124	-0.10326217715465447
1103	125-129	-0.16599328179292883
1103	130-134	-0.09571832635575106
1103	135-139	-0.11103370363503018
1103	140-144	-0.09182873212368747
1103	145-149	-0.037927814060516596
1103	150-154	-0.11884508101479696
1103	155-159	-0.17289127549086913
1103	160-164	-0.1373904802788033
1103	165-169	-0.14157321721911842
1103	170-174	-0.21910645698039843
1103	175-179	-0.11450422932771431
1103	180-184	-0.13500107553834795
1103	185-189	-0.0771619474897065
1103	190-194	-0.187937671337167
1103	195-199	-0.16445593314866613
1103	200-204	0.076322218775843
1103	205-209	-0.13130917705148448
1103	210-214	-0.21234839513598303
1103	215-219	-0.31049001762778516
1103	220-224	-0.3819561368851012
1103	225-229	-0.14583400334789332
1103	230-234	-0.39288203608130345
1103	235-239	-0.2312481483902573
1103	240-244	-0.29631820489348115
1103	245-249	-0.257360261819251
1103	250-254	-0.48675805355305357
1103	255-259	-0.2383718359780076
1103	260-264	-0.3245483452128326
1103	265-269	-0.4338240828561837
1103	270-274	-0.33382345130632274
1103	275-279	-0.19410428470683172
1103	280-284	-0.43483455660586046
1103	285-289	-0.5373150155447526
1103	290-294	-0.40481917713851345
1103	295-299	-0.7645778366561267
1103	300-301	-1.0509727351422882
1104	1	0.09570223048481097
1104	2	0.11795311136663145
1104	3	0.11118667148066663
1104	4	-0.15253172371773616
1104	5	-0.274295662381455
1104	6	0.20175361342375453
1104	7	-0.1072808133767893
1104	8	0.08006886694415982
1104	9	-0.03623038284008828
1104	10-14	-0.10375347399961043
1104	15-19	-0.12105835529419551
1104	20-24	-0.1356836766510483
1104	25-29	-0.027933515848751256
1104	30-34	-0.08023856314177635
1104	35-39	-0.027397557374037262
1104	40-44	-0.0961759795000603
1104	45-49	-0.1471907338385492
1104	50-54	-0.07951130321712441
1104	55-59	-0.10751823353840706
1104	60-64	-0.03278760534479375
1104	65-69	-0.1357756895839728
1104	70-74	-0.12038041054783832
1104	75-79	-0.15414527677058487
1104	80-84	-0.0901346415767037
1104	85-89	-0.14072069899160056
1104	90-94	-0.20354012367578633
1104	95-99	-0.09190980990494069
1104	100-104	-0.23061044294213673
1104	105-109	-0.16337484824325088
1104	110-114	-0.12079523234763911
1104	115-119	-0.3439709619514417
1104	120-124	-0.3035972643881166
1104	125-129	-0.25376582864053177
1104	130-134	-0.23107006870196756
1104	135-139	-0.30047349936932477
1104	140-144	-0.304920054993957
1104	145-149	-0.31508776756311363
1104	150-154	-0.40557868692621923
1104	155-159	-0.37237578767806667
1104	160-164	-0.28744935343728173
1104	165-169	-0.15505007126004244
1104	170-174	-0.3845270523873694
1104	175-179	-0.2285336566355909
1104	180-184	-0.20570727529914734
1104	185-189	-0.2576874483751226
1104	190-194	-0.16476018962492844
1104	195-199	-0.5584721951203093
1104	200-204	0.0163151725395565
1104	205-209	-0.4635457080780938
1104	210-214	-0.38214970981533014
1104	215-219	-0.19420843613279715
1104	220-224	-0.23981617674628808
1104	225-229	-0.2074664535097952
1104	230-234	-0.36119657705051367
1104	235-239	-0.3143681997829688
1104	240-244	-0.4038222786399359
1104	245-249	-0.29661162361448845
1104	250-254	-0.2896566987413749
1104	255-259	-0.6557367039886195
1104	260-264	-0.5645101683889493
1104	265-269	-0.4650480690752872
1104	270-274	-0.6907534759815839
1104	275-279	-0.7553501527640094
1104	280-284	-0.6934410564889575
1104	285-289	-0.8853695928916778
1104	290-294	-0.9122969163830525
1104	295-299	-0.901186558110993
1104	300-301	-1.6181765824273988
1201	1	-0.5934282042977976
1201	2	-0.17987297558989468
1201	3	-0.04533506764976636
1201	4	0.0887726241083513
1201	5	0.11918259848810919
1201	6	-0.34389856048928635
1201	7	-0.022498204681134837
1201	8	-0.08297461131671469
1201	9	-0.24275212197052554
1201	10-14	-0.04288390878222259
1201	15-19	-0.07671052920723298
1201	20-24	-0.0695967201293044
1201	25-29	-0.040542211500927294
1201	30-34	-0.08502117183742541
1201	35-39	-0.07435407911317071
1201	40-44	-0.16834989254353872
1201	45-49	-0.10936464688203529
1201	50-54	-0.14777217278234644
1201	55-59	-0.09882258136449451
1201	60-64	-0.30017890969261884
1201	65-69	-0.059253950453538096
1201	70-74	-0.24559780185219182
1201	75-79	-0.1911017985097203
1201	80-84	-0.11839551114192659
1201	85-89	-0.12376417725247535
1201	90-94	-0.2491922975888201
1201	95-99	-0.10625763599190208
1201	100-104	-0.15061044294214554
1201	105-109	-0.2629400656345595
1201	110-114	-0.19731697147808092
1201	115-119	-0.10614487499491787
1201	120-124	-0.19055378612724638
1201	125-129	-0.29898321994487276
1201	130-134	-0.21362287726501705
1201	135-139	-0.21264267621880606
1201	140-144	-0.334998675814262
1201	145-149	-0.22035650417405606
1201	150-154	-0.29659824265054624
1201	155-159	-0.1820890969622866
1201	160-164	-0.21675446070896953
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2404	260-264	0.6567176842766287
2404	265-269	0.6136020734283392
2404	270-274	0.6674685135368676
2404	275-279	0.6700713072436031
2404	280-284	0.7754617788247131
2404	285-289	1.005492726638625
2404	290-294	0.958010658123964
2404	295-299	1.0909161927791189
2404	300-301	1.3076298280903345
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	16.0
17	41.0
18	65.0
19	127.0
20	203.0
21	300.0
22	480.0
23	515.0
24	515.0
25	601.0
26	657.0
27	819.0
28	1082.0
29	1579.0
30	2308.0
31	4273.0
32	12207.0
33	109963.0
34	3543.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.2728042844630781	0.0696368831392594	0.1170186799144256	99.54054015248323
2	0.06030497085259742	0.14789076185279845	0.2627573730006031	99.529046894294
3	0.1392737662785188	0.19885996525334906	99.47018536333223	0.1916809051358996
4	0.11414705586744583	99.48167185952015	0.20460321334730855	0.19957787126509396
5	0.1981420592416041	0.21608970953522766	0.20029577727683892	99.38547245394633
6	99.41275288239264	0.22470458167616697	0.05240713885738079	0.3101353970738151
7	99.79683259867618	0.07609803724496389	0.05240713885738079	0.074662225221474
8	99.54771921260068	0.12635145806710985	0.09835312360905711	0.2275762057231467
9	31.12122560914325	22.089967981391876	25.0886613924505	21.70014501701437
10-14	59.707668672017455	10.45285511220871	9.613910146883569	20.22556606889026
15-19	29.335201811428252	30.51128050575261	19.82678289136456	20.326734791454584
20-24	59.90882528195961	39.30577990279483	0.5361360585241183	0.24925875672144332
25-29	8.692924103649439	9.084186812508346	59.89722354238488	22.325665541457333
30-34	3.4319419576513694	57.40885654103556	0.6699623953811936	38.48923910593188
35-39	54.204143405242725	10.221672754841823	19.733297723252235	15.840886116663219
40-44	35.75947140233193	47.420415232367276	0.48583077349486525	16.33428259180593
45-49	20.20789614672521	19.93470389090695	29.86600365118146	29.991396311186374
50-54	50.04546205390808	20.417130504104513	17.841665649216804	11.695741792770601
55-59	21.034086501860177	37.03822342243992	3.1305571914905843	38.79713288420932
60-64	40.04013537429037	0.21777103587763452	0.7265738123146935	59.0155197775173
65-69	39.29279434580245	21.48106648279032	36.327788312359935	2.898350859047291
70-74	40.38772329284673	56.010429311248856	3.403891598370959	0.19795579753345352
75-79	40.99045517495015	20.999189752960852	26.190804558070578	11.819550514018424
80-84	39.2989725082751	1.2422708256712025	39.18131091393895	20.277445752114748
85-89	25.197046104334582	13.104032872146314	45.7338045773889	15.965116446130196
90-94	17.967591581512433	58.64693847055713	4.8920102529914145	18.49345969493902
95-99	15.931421148977382	3.326781213808879	60.15535420240876	20.586443434804973
100-104	29.489012702468496	13.5477668287229	38.89478895564986	18.068431513158746
105-109	47.80782308525835	40.517476769018074	9.898424633319824	1.776275512403749
110-114	46.802257061221205	47.018851002914744	3.3900473143501033	2.788844621513944
115-119	29.422952516496316	43.53790198530786	19.478874656776064	7.5602708414197615
120-124	44.04856052523359	23.30767173476049	23.244835806988736	9.39893193301719
125-129	14.378869704661984	35.00751468779888	30.796700728457292	19.816914879081843
130-134	36.00085741331638	22.887756540653765	18.671901299642936	22.439484746386924
135-139	10.72915242775024	20.663467556853746	42.96078332625884	25.646596689137173
140-144	39.61054193416544	17.522446870822016	19.09988012025392	23.76713107475862
145-149	11.206429994357633	38.824660594406	33.09348479439909	16.875424616837282
150-154	17.97040564820787	23.303320510457937	12.739565097208144	45.98670874412605
155-159	25.335078375728358	62.30319679672416	0.40933626477418833	11.952388562773294
160-164	27.24332528378614	14.441665262675876	10.550954785736606	47.76405466780138
165-169	35.97615241715989	26.078109474229922	23.439178583257732	14.506559525352458
170-174	7.676841870278755	36.98934952148459	21.397596261170758	33.9362123470659
175-179	53.012228671842365	5.280222391864836	16.25376294525185	25.45378599104095
180-184	36.19687490546495	35.985260455756155	3.3241763386522654	24.493688300126625
185-189	36.41842612577047	4.890657553115157	18.430304095623132	40.26061222549124
190-194	25.58058101331489	30.461842181105542	30.509971756297194	13.44760504928238
195-199	17.68742091412463	40.2649791170053	25.420037874748154	16.62756209412192
200-204	69.26032800197207	2.246421994402796	23.029305569653292	5.46394443397184
205-209	23.322248025474586	41.3349363063293	8.879634314577684	26.463181353618438
210-214	45.10410269274352	13.34045516274966	39.42884081413755	2.1266013303692684
215-219	40.05772829638088	18.74872043299106	10.611827668636119	30.581723601991946
220-224	23.348492101483963	17.69509824842693	51.71538235277154	7.241027297317561
225-229	48.01244716946579	4.785354099764813	28.475495716599063	18.726703014170333
230-234	20.76329668713143	51.82704607960634	22.141603462424303	5.2680537708379305
235-239	51.87957128207199	22.44812302491047	8.775275016118028	16.89703067689951
240-244	34.231939289645	29.99052302242019	11.266929098648557	24.510608589286257
245-249	44.61456563074358	20.9678489712421	12.46930749632844	21.948277901685888
250-254	29.17546806103839	30.468695893067387	27.643881155204653	12.711954890689567
255-259	14.545727409027876	38.36435604131102	18.793281836455805	28.2966347132053
260-264	36.76244768364844	20.888439890316064	32.94458074758263	9.40453167845288
265-269	23.188242629992725	50.744795094744866	15.468787889285343	10.598174385977067
270-274	18.24724259398279	7.9911358780389214	30.382860772651156	43.378760755327136
275-279	30.98202914956842	21.603284019140414	18.27262672438439	29.14206010690678
280-284	10.73756689859141	35.88753816071836	21.41722481681411	31.95767012387612
285-289	42.578504709343726	33.108109084028676	15.497431201406231	8.81595500522137
290-294	31.834748804950948	20.660155352765187	21.270645203696454	26.234450638587408
295-299	40.19435395680123	25.777068663245174	17.6777334822281	16.350843897725497
300-301	25.139570972158833	69.57151985394798	5.166590597900502	0.1223185759926974
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	0.5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.5
21	0.8333333333333333
22	0.0
23	0.5833333333333333
24	1.0833333333333333
25	1.1666666666666665
26	1.0833333333333333
27	2.25
28	3.5
29	3.4166666666666665
30	1.5
31	1.3333333333333333
32	1.8333333333333333
33	1.75
34	2.0
35	1.25
36	0.0
37	0.5833333333333333
38	0.0
39	0.25
40	0.75
41	1.1666666666666665
42	2.583333333333333
43	3.666666666666667
44	7.083333333333332
45	17.16666666666667
46	22.583333333333336
47	28.416666666666657
48	46.833333333333336
49	222.25000000000014
50	3751.166666666672
51	4356.833333333345
52	14267.750000000085
53	4577.833333333334
54	4955.583333333329
55	9729.083333333336
56	2837.6666666666715
57	637.4166666666666
58	160.58333333333334
59	94.58333333333333
60	69.08333333333336
61	36.33333333333333
62	19.333333333333336
63	9.833333333333332
64	6.833333333333333
65	4.333333333333333
66	1.3333333333333333
67	1.0
68	1.0
69	1.25
70	1.25
71	1.5
72	1.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0014358120234898848
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	1.440069353740076E-4
170-174	0.0
175-179	0.0
180-184	2.8810597690254386E-4
185-189	0.0
190-194	0.0
195-199	2.882392616463074E-4
200-204	0.6024704169729465
205-209	0.0
210-214	0.0
215-219	1.441763565192944E-4
220-224	0.0
225-229	1.4419777012568278E-4
230-234	0.0
235-239	0.0
240-244	0.0
245-249	1.4426537905007017E-4
250-254	0.0
255-259	0.0
260-264	0.0
265-269	1.4433992630003364E-4
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
10-19	1.0
20-29	1.0
30-39	12.0
40-49	42.0
50-59	6.0
60-69	9.0
70-79	9.0
80-89	10.0
90-99	32.0
100-109	5.0
110-119	61.0
120-129	69.0
130-139	48.0
140-149	68.0
150-159	26.0
160-169	18.0
170-179	31.0
180-189	48.0
190-199	30.0
200-209	32.0
210-219	24.0
220-229	29.0
230-239	25.0
240-249	36.0
250-259	35.0
260-269	42.0
270-279	43.0
280-289	46.0
290-299	1105.0
300-302	137351.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.787083435036685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.778506452295325	4.125087943486438
2	14.068119314575842	1.9096299912415466
3	6.6744235244341015	1.3589960802331758
4	4.220435794372753	1.145777994744928
5	2.654960863126719	0.9009720447399027
6	2.168394330442141	0.8830243944462791
7	1.5760524645652634	0.7487759702499749
8	1.0471757986037655	0.5685815613019943
9	0.8250475988999365	0.5039700202449495
>10	4.75988999365348	5.840165405545106
>50	0.3490585995345885	1.7229744281878616
>100	0.35963613285381846	6.0648699872212735
>500	0.15866299978844933	7.945065831981276
>1k	0.2855933996192088	34.47384668399213
>5k	0.07404273323460969	31.80826166238316
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	8092	5.809295447040073	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	6953	4.991600499662584	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	6393	4.589573133085416	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	6140	4.407942912113946	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	5955	4.275130299941131	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	5658	4.061912214452884	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	5116	3.672807156087125	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4711	3.382055221330423	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2773	1.990753370568725	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2490	1.7875859692449065	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2226	1.5980587821442416	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2205	1.5829827558975977	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2125	1.5255502749580026	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2120	1.5219607448992778	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2018	1.4487343317012937	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1994	1.4315045874194152	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1804	1.295102445187876	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1751	1.257053426565394	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1721	1.2355162462130458	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1713	1.2297729981190864	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1709	1.2269013740721064	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1685	1.2096716297902277	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1557	1.1177796602868753	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1456	1.045271153100636	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1455	1.0445532470888912	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1311	0.9411747813976193	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1307	0.9383031573506397	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1284	0.921791319080506	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1240	0.8902034545637285	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1149	0.8248740074949388	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1088	0.7810817407784972	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1069	0.7674415265553434	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1053	0.7559550303674243	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1006	0.7222134478154121	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	909	0.6525765646761525	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	896	0.6432437865234684	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	881	0.6324751963472942	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	837	0.6008873318305168	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	817	0.5865292115956179	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	810	0.5815038695134034	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	755	0.5420190388674315	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	750	0.5384295088087068	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	701	0.5032521142332046	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	696	0.4996625841744799	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	665	0.47740749781038666	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	646	0.4637672835872328	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	618	0.4436659152583744	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	552	0.3962841184832082	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	534	0.3833618102717992	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	408	0.2929056527919365	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	405	0.2907519347567017	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	395	0.28357287463925224	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	381	0.273522190474823	No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	370	0.2656252243456287	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	336	0.24121641994630066	No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	333	0.23906270191106582	No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	323	0.2318836417936164	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	283	0.2031674013238187	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	277	0.19885996525334906	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	271	0.19455252918287938	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	266	0.19096299912415465	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	259	0.18593765704194007	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	258	0.1852197510301951	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	237	0.17014372478355133	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	236	0.1694258187718064	No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	233	0.16727210073657156	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	228	0.16368257067784686	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	225	0.16152885264261202	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	219	0.15722141657214236	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	218	0.15650351056039744	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	215	0.1543497925251626	No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	212	0.15219607448992778	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	212	0.15219607448992778	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	205	0.14717073240771317	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	200	0.14358120234898847	No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	190	0.13640214223153904	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	171	0.12276192800838515	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	159	0.11414705586744583	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	156	0.111993337832211	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	155	0.11127543182046606	No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	150	0.10768590176174135	No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	147	0.10553218372650654	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
