##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IAMS2_cleaned_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	150412
Sequences flagged as poor quality	0
Sequence length	51-301
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.713932399010716	40.0	40.0	40.0	40.0	40.0
2	39.337752306996784	40.0	40.0	40.0	40.0	40.0
3	39.35433343084328	40.0	40.0	40.0	40.0	40.0
4	39.061085551684705	40.0	40.0	40.0	40.0	40.0
5	39.22386511714491	40.0	40.0	40.0	40.0	40.0
6	39.41392973964843	40.0	40.0	40.0	40.0	40.0
7	39.398452251150175	40.0	40.0	40.0	40.0	40.0
8	39.46150573092572	40.0	40.0	40.0	40.0	40.0
9	39.082972103289634	40.0	40.0	40.0	40.0	40.0
10-14	39.61653591468765	40.0	40.0	40.0	40.0	40.0
15-19	39.51735233890913	40.0	40.0	40.0	40.0	40.0
20-24	39.464064037443826	40.0	40.0	40.0	40.0	40.0
25-29	39.646735632795256	40.0	40.0	40.0	40.0	40.0
30-34	39.54148339228253	40.0	40.0	40.0	40.0	40.0
35-39	39.638837326809025	40.0	40.0	40.0	40.0	40.0
40-44	39.60147328670585	40.0	40.0	40.0	40.0	40.0
45-49	39.54178124085844	40.0	40.0	40.0	40.0	40.0
50-54	39.53003597311412	40.0	40.0	40.0	40.0	40.0
55-59	39.47782010624223	40.0	40.0	40.0	40.0	40.0
60-64	39.44604583441371	40.0	40.0	40.0	40.0	40.0
65-69	39.47123016268757	40.0	40.0	40.0	40.0	40.0
70-74	39.47155682454298	40.0	40.0	40.0	40.0	40.0
75-79	39.50772422046407	40.0	40.0	40.0	40.0	40.0
80-84	39.479300578418986	40.0	40.0	40.0	40.0	40.0
85-89	39.62613124127385	40.0	40.0	40.0	40.0	40.0
90-94	39.51727145801476	40.0	40.0	40.0	40.0	40.0
95-99	39.43064955787514	40.0	40.0	40.0	40.0	40.0
100-104	39.36031750543284	40.0	40.0	40.0	40.0	40.0
105-109	39.42593929723081	40.0	40.0	40.0	40.0	40.0
110-114	39.43451879924204	40.0	40.0	40.0	40.0	40.0
115-119	39.35812772181776	40.0	40.0	40.0	40.0	40.0
120-124	39.32418204182042	40.0	40.0	40.0	40.0	40.0
125-129	39.40123267178618	40.0	40.0	40.0	40.0	40.0
130-134	39.408280309830126	40.0	40.0	40.0	40.0	40.0
135-139	39.15827798277983	40.0	40.0	40.0	40.0	40.0
140-144	39.486965772186906	40.0	40.0	40.0	40.0	40.0
145-149	39.15185766336002	40.0	40.0	40.0	40.0	40.0
150-154	39.279141512193824	40.0	40.0	40.0	40.0	40.0
155-159	39.29956118188346	40.0	40.0	40.0	40.0	40.0
160-164	39.28564133932608	40.0	40.0	40.0	40.0	40.0
165-169	39.32278396851147	40.0	40.0	40.0	40.0	40.0
170-174	39.17761761655275	40.0	40.0	40.0	40.0	40.0
175-179	39.42926517911758	40.0	40.0	40.0	40.0	40.0
180-184	39.3394537379325	40.0	40.0	40.0	40.0	40.0
185-189	39.262306853541126	40.0	40.0	40.0	40.0	40.0
190-194	39.34859179277147	40.0	40.0	40.0	40.0	40.0
195-199	39.31550756628813	40.0	40.0	40.0	40.0	40.0
200-204	39.440488284886044	40.0	40.0	40.0	40.0	40.0
205-209	39.302167495545326	40.0	40.0	40.0	40.0	40.0
210-214	39.36495305975905	40.0	40.0	40.0	40.0	40.0
215-219	39.424972740086695	40.0	40.0	40.0	40.0	40.0
220-224	39.31745432302332	40.0	40.0	40.0	40.0	40.0
225-229	39.37878658387877	40.0	40.0	40.0	40.0	40.0
230-234	39.24020212765957	40.0	40.0	40.0	40.0	40.0
235-239	39.31701595744681	40.0	40.0	40.0	40.0	40.0
240-244	39.33562765957447	40.0	40.0	40.0	40.0	40.0
245-249	39.12796276595745	40.0	40.0	40.0	40.0	40.0
250-254	39.33188829787234	40.0	40.0	40.0	40.0	40.0
255-259	39.05957978723405	40.0	40.0	40.0	40.0	40.0
260-264	39.0583085106383	40.0	40.0	40.0	40.0	40.0
265-269	38.974202127659574	40.0	40.0	40.0	40.0	40.0
270-274	38.406271276595746	40.0	40.0	40.0	36.8	40.0
275-279	38.93187234042553	40.0	40.0	40.0	40.0	40.0
280-284	38.664255319148936	40.0	40.0	40.0	36.8	40.0
285-289	39.051739361702126	40.0	40.0	40.0	40.0	40.0
290-294	39.032872636694194	40.0	40.0	40.0	40.0	40.0
295-299	39.02268795837331	40.0	40.0	40.0	40.0	40.0
300-301	38.4879032646507	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.06227588529716854
1101	2	-0.2121131852595326
1101	3	0.17559695364167283
1101	4	0.36532609493806234
1101	5	0.1401000534655381
1101	6	0.22405706238306067
1101	7	0.04862053568474778
1101	8	0.07830649682320256
1101	9	0.37575893262318516
1101	10-14	0.12639432216682422
1101	15-19	0.11609783246397853
1101	20-24	0.13093448423003196
1101	25-29	0.10109390400934615
1101	30-34	0.17700367704425446
1101	35-39	0.05622578661951394
1101	40-44	0.16355578242964697
1101	45-49	0.08703705264834127
1101	50-54	0.07276816967898014
1101	55-59	0.08749667831319385
1101	60-64	0.05360762436438904
1101	65-69	0.18521994807384345
1101	70-74	0.14504286429048818
1101	75-79	0.13713810185298314
1101	80-84	0.14167762727920064
1101	85-89	0.045129116109492884
1101	90-94	0.11129912522525132
1101	95-99	0.1079372530870657
1101	100-104	0.174226382003134
1101	105-109	0.23335701564570854
1101	110-114	0.15516173412459722
1101	115-119	0.1231851898651044
1101	120-124	0.22304552934915733
1101	125-129	0.17060284683977045
1101	130-134	0.09597589726435984
1101	135-139	0.12994533068107472
1101	140-144	0.07681827185431445
1101	145-149	0.18014663094053418
1101	150-154	0.08998374432982104
1101	155-159	0.16171201773805421
1101	160-164	0.12528709276521965
1101	165-169	0.15739832364717188
1101	170-174	0.19953690491849585
1101	175-179	0.20932028773356137
1101	180-184	0.05688306602231563
1101	185-189	0.10243866686055725
1101	190-194	0.03478465120604568
1101	195-199	0.1057630555976985
1101	200-204	0.12893866987113967
1101	205-209	0.08258526205530359
1101	210-214	0.07324096289485738
1101	215-219	0.24280420079841036
1101	220-224	0.17449500672356066
1101	225-229	0.21322345652717445
1101	230-234	0.14092506154218398
1101	235-239	0.21023107985654832
1101	240-244	0.11362080089683246
1101	245-249	0.028560380588764644
1101	250-254	0.21100546742044202
1101	255-259	0.1889882308308799
1101	260-264	0.22623287127476743
1101	265-269	0.1182138789358973
1101	270-274	0.3259291294887774
1101	275-279	0.19841611343520782
1101	280-284	0.12874931507477783
1101	285-289	0.17710960072518134
1101	290-294	0.05098878907732285
1101	295-299	0.06769914672398869
1101	300-301	0.03814807662639197
1102	1	0.06227588529716854
1102	2	0.060431904920832835
1102	3	0.15956488951341896
1102	4	0.24508561397613704
1102	5	0.22026037410682164
1102	6	0.16794483793416504
1102	7	0.16886101664666597
1102	8	0.022194272374306934
1102	9	0.37575893262318516
1102	10-14	0.06386927206662563
1102	15-19	0.026318273345736998
1102	20-24	0.039551718698966454
1102	25-29	-0.017543370539755188
1102	30-34	0.024699067825828536
1102	35-39	0.06744823150929591
1102	40-44	0.06896660407292643
1102	45-49	0.058179337217481475
1102	50-54	-0.086515860228225
1102	55-59	0.020757577621850487
1102	60-64	0.10560559621169574
1102	65-69	-0.005027212914797019
1102	70-74	0.08643890939274002
1102	75-79	0.05930854716127243
1102	80-84	0.10717649650359817
1102	85-89	0.08461370778280042
1102	90-94	0.009321676351603969
1102	95-99	0.07002689421670283
1102	100-104	-0.03072971492968435
1102	105-109	0.0687965694521111
1102	110-114	0.09325800699162556
1102	115-119	0.08682009018783532
1102	120-124	0.13858101799714007
1102	125-129	0.017171486126372315
1102	130-134	0.0917554080932419
1102	135-139	0.12366771520915876
1102	140-144	0.066333044371234
1102	145-149	0.19485878617469155
1102	150-154	-0.02374089370125887
1102	155-159	-0.0771399834530726
1102	160-164	-0.08952647010348613
1102	165-169	0.02783155959699002
1102	170-174	0.12275683876208632
1102	175-179	0.15878065424972476
1102	180-184	0.007450868293531698
1102	185-189	0.06742244968724975
1102	190-194	-0.02110544223384636
1102	195-199	0.07245950069673057
1102	200-204	0.042912802876116984
1102	205-209	0.023498413659709172
1102	210-214	0.06887238638440607
1102	215-219	0.08631451459869766
1102	220-224	0.05780218332575515
1102	225-229	0.09028842984730545
1102	230-234	0.1702206004110849
1102	235-239	0.0036814343808586614
1102	240-244	-0.09309946694809668
1102	245-249	0.18749672717752475
1102	250-254	0.03501332248801248
1102	255-259	0.03809045133614575
1102	260-264	0.06090945335458997
1102	265-269	-0.08438247843749025
1102	270-274	-0.04605982834657141
1102	275-279	-0.15510699704278608
1102	280-284	-0.0021437964494737116
1102	285-289	0.08398519931191117
1102	290-294	-0.010277518638517336
1102	295-299	-0.06817213764797003
1102	300-301	-0.31195718183870014
1103	1	-7.003666078375659E-4
1103	2	0.0708479465849976
1103	3	0.24145321706672718
1103	4	-0.11917184305368522
1103	5	0.2861806377878793
1103	6	-0.006167858516619162
1103	7	-0.037091807164628676
1103	8	0.13605072780012506
1103	9	0.010605472009338257
1103	10-14	0.04120998142946064
1103	15-19	-0.016555498149344317
1103	20-24	0.04324133345743064
1103	25-29	-0.014480958290107537
1103	30-34	0.07431846630340999
1103	35-39	0.02391525737738931
1103	40-44	0.05277133929187272
1103	45-49	0.013548758695165475
1103	50-54	0.06957775691732593
1103	55-59	0.04612371282132699
1103	60-64	0.06680127713899253
1103	65-69	-0.04015695343375825
1103	70-74	0.09388425965605762
1103	75-79	-0.05783547804133349
1103	80-84	-0.08354567361399745
1103	85-89	-0.014205521229051499
1103	90-94	0.07610858446308555
1103	95-99	-0.0019255863643010684
1103	100-104	-0.1272980240944861
1103	105-109	0.01280253342777371
1103	110-114	0.006770740560625654
1103	115-119	-0.028073015167720428
1103	120-124	0.0029390489232383743
1103	125-129	-0.011397881163134116
1103	130-134	0.0692365903071277
1103	135-139	-0.09709557748256259
1103	140-144	-0.026887742969748274
1103	145-149	-0.03752463974455367
1103	150-154	-9.661599161603363E-5
1103	155-159	-0.1299115487561977
1103	160-164	-0.13109153274927365
1103	165-169	-0.01775437696362303
1103	170-174	-0.013491147193711583
1103	175-179	-0.09579453272572636
1103	180-184	-0.10352717561864466
1103	185-189	-0.06267879360928674
1103	190-194	-0.06009252830267542
1103	195-199	-0.0675496376530802
1103	200-204	-0.01940112348803069
1103	205-209	-0.0985514624915993
1103	210-214	0.021247154919620925
1103	215-219	-0.09439394799418466
1103	220-224	-0.20190009885686777
1103	225-229	0.020096284018478627
1103	230-234	-0.13128722730697007
1103	235-239	-0.12006144131352414
1103	240-244	-0.09497523348730397
1103	245-249	-0.07184002101283937
1103	250-254	-0.018758651636034074
1103	255-259	-0.002839736480993338
1103	260-264	0.013582420672967999
1103	265-269	0.06269685686780235
1103	270-274	-0.2119930221998274
1103	275-279	-0.04887127571435457
1103	280-284	-0.12839731351174066
1103	285-289	-0.10083071103607466
1103	290-294	-0.1561544394955945
1103	295-299	-0.16680936192426543
1103	300-301	-0.12025259961961154
1104	1	-0.0431721241237355
1104	2	-0.04787543140129458
1104	3	-0.028439986217421165
1104	4	-0.22202592869683713
1104	5	0.07349261177804323
1104	6	-0.016357787936129853
1104	7	0.15706009521618114
1104	8	-0.03408323108622113
1104	9	0.21203354370101124
1104	10-14	0.03802624580792724
1104	15-19	0.10870655506381866
1104	20-24	-0.03331318636890046
1104	25-29	0.1153640938736018
1104	30-34	-0.045435427969884756
1104	35-39	0.08862377847952985
1104	40-44	0.02112494136606813
1104	45-49	0.04935343799635916
1104	50-54	0.13377595845240364
1104	55-59	0.03697088445743901
1104	60-64	-0.022000912296562092
1104	65-69	0.061501996707200135
1104	70-74	-0.04420821518806406
1104	75-79	0.008008915645518755
1104	80-84	-0.11181079286004092
1104	85-89	0.0139674112564947
1104	90-94	-0.11094164497494319
1104	95-99	-0.06754939030281548
1104	100-104	-0.01083322174042678
1104	105-109	-0.06708717169805567
1104	110-114	-0.02544194746538153
1104	115-119	0.12991204563850545
1104	120-124	0.029877337294948347
1104	125-129	0.1274617192237244
1104	130-134	0.12437744262312123
1104	135-139	-0.0064825766800424844
1104	140-144	0.06464712543776585
1104	145-149	-0.028980902476817505
1104	150-154	0.022173071437649128
1104	155-159	-0.09493984268381439
1104	160-164	-0.07446581511239003
1104	165-169	-0.18928101441038336
1104	170-174	-0.12893860279796598
1104	175-179	-0.09658141257426678
1104	180-184	-0.1852639083387757
1104	185-189	-0.13701095403070696
1104	190-194	-0.2226704652123601
1104	195-199	-0.10246866557661605
1104	200-204	-0.009148638766745876
1104	205-209	-0.14164877302516743
1104	210-214	0.008792065099264335
1104	215-219	-0.15605372123121697
1104	220-224	-0.023406776223787062
1104	225-229	-0.14039098885844226
1104	230-234	-0.06804710960423677
1104	235-239	-0.15608671036305566
1104	240-244	-0.27164457521621443
1104	245-249	-0.05699795257239515
1104	250-254	-0.06243212942272436
1104	255-259	-0.28123716842123514
1104	260-264	-0.2715709838581759
1104	265-269	-0.2506558192233115
1104	270-274	-0.33697193036288553
1104	275-279	-0.17099239430191204
1104	280-284	-0.2172027953316089
1104	285-289	-0.15044365010638927
1104	290-294	-0.1435446076129736
1104	295-299	-0.13147000875655124
1104	300-301	-0.45692295757508106
1201	1	-0.09032439723510777
1201	2	-0.1746119532873891
1201	3	-0.3028188944479453
1201	4	0.006789349315674542
1201	5	-0.12736931149388653
1201	6	-0.2423675902007929
1201	7	-0.16002626611557957
1201	8	-0.08791118444096213
1201	9	-0.02141245934107161
1201	10-14	-0.1565453276245563
1201	15-19	-0.025036896009368093
1201	20-24	-0.15095388266770016
1201	25-29	-0.09958942987153563
1201	30-34	-0.034166203700834785
1201	35-39	-0.08182408361275861
1201	40-44	-0.0879472235823826
1201	45-49	-0.18571500885170877
1201	50-54	0.007996002393589663
1201	55-59	-0.161677079037581
1201	60-64	-0.016792191661110678
1201	65-69	-0.10775945335196013
1201	70-74	-0.21608923597563034
1201	75-79	0.050354698981664114
1201	80-84	-0.07348055794369657
1201	85-89	-0.04934507818072831
1201	90-94	-0.10557067995888048
1201	95-99	-0.035654849150191126
1201	100-104	-0.14124390282881905
1201	105-109	-0.1860325832241685
1201	110-114	-0.2200608421755348
1201	115-119	-0.19486402331536112
1201	120-124	-0.2955950304589763
1201	125-129	-0.07959591133231214
1201	130-134	-0.33500738076711656
1201	135-139	-0.20937593480071115
1201	140-144	-0.21137450714631
1201	145-149	-0.3629001182956486
1201	150-154	-0.216130122748595
1201	155-159	-0.15228647104277826
1201	160-164	-0.28608318878147543
1201	165-169	-0.2954023596867046
1201	170-174	-0.19185900164873715
1201	175-179	-0.2842373192837542
1201	180-184	-0.24555459618735398
1201	185-189	-0.4430917382118835
1201	190-194	-0.2499816026130688
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2404	215-219	0.133404770358581
2404	220-224	0.1788894798803895
2404	225-229	0.17002463783715882
2404	230-234	0.1353256521971744
2404	235-239	0.1380597899082332
2404	240-244	0.13820892451249023
2404	245-249	0.022680832017940133
2404	250-254	-0.006801093068951047
2404	255-259	0.14955610341815628
2404	260-264	0.12361078499694145
2404	265-269	0.07477879824819667
2404	270-274	0.2762196051769905
2404	275-279	0.08787249989233459
2404	280-284	0.14636095941609284
2404	285-289	0.02674571505909995
2404	290-294	0.06561003156228651
2404	295-299	0.17561192287666216
2404	300-301	0.07715776040623012
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	12.0
21	33.0
22	41.0
23	67.0
24	216.0
25	249.0
26	265.0
27	312.0
28	304.0
29	357.0
30	450.0
31	520.0
32	716.0
33	803.0
34	1061.0
35	1309.0
36	1896.0
37	2949.0
38	5952.0
39	116231.0
40	16669.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.070473100550488	0.00598356514107917	0.08775895540249448	99.83578437890593
2	0.01263205483641489	0.009307829879463603	0.15424403800253972	99.82381607728158
3	0.0106374491396963	0.1276493896763556	99.6270244395394	0.23468872164454962
4	0.07978086854772225	99.40962157274686	0.10570965082573199	0.4048879078796905
5	0.1941334467994575	0.15025396909821026	0.08775895540249448	99.56785362869984
6	99.76797064064039	0.0671488976943329	0.0106374491396963	0.15424301252559636
7	99.9055926388852	0.05584660798340558	0.025263941706778714	0.013296811424620377
8	99.73938249607744	0.10371512911203894	0.08642927426003244	0.070473100550488
9	29.155918410765096	21.187139323990106	26.58099087838736	23.075951386857433
10-14	59.823285376166794	10.435204637927825	9.59085711246443	20.15065287344095
15-19	39.55402494481823	20.176714623833206	19.962104087439833	20.30715634390873
20-24	61.29843363561418	38.4430763502912	0.22843922027497807	0.030050793819642048
25-29	19.61864744834189	19.792702709890168	59.77169374783927	0.8169560939286759
30-34	4.274791904901205	57.50192803765657	0.3357444884716645	37.88753556897056
35-39	64.37106081961545	1.4329973672313379	19.837778900619632	14.358162912533576
40-44	34.853469138100685	49.11296970986358	0.08669521048852485	15.946865941547216
45-49	20.08682817860277	19.909847618541075	21.394968486556923	38.60835571629923
50-54	38.35241211769853	20.1650938692147	17.651986485066956	23.83050752801982
55-59	23.44549268338087	27.62736767922559	9.306899096475657	39.620240540917884
60-64	39.97978871226174	0.07924952297371868	0.3316246817054604	59.60933708305909
65-69	32.64189454228747	21.465318361024128	42.049717108456164	3.8430699882322434
70-74	40.03132766261863	55.40156186216094	4.454485134651772	0.11262534056865825
75-79	36.93065620636926	22.257562662057044	12.082175387274782	28.729605744298915
80-84	40.002792367528755	0.1635529552556346	39.29353101522505	20.54012366199056
85-89	34.00811116282162	6.039890964696496	55.38900339073199	4.562994481749884
90-94	15.289541918755404	61.19579815171864	10.814972408749417	12.699687520776545
95-99	6.375905857323316	12.24120736653148	61.24632670700086	20.136560069144338
100-104	25.22439264391048	22.90839461191708	10.428706301610308	41.43850644256214
105-109	63.91236993451016	33.00581762574383	0.8247066254446328	2.2571058143013865
110-114	40.35889764303048	51.86383431401882	5.76097869086799	2.01628935208271
115-119	38.30324789734384	31.847212526179312	19.61530534224261	10.234234234234235
120-124	23.346963199361724	28.459692164489216	33.30195139789236	14.891393238256706
125-129	21.37734782753233	39.82287822878229	22.04846913333998	16.751304810345403
130-134	23.51956384428709	38.026395399089125	26.463215983511184	11.990824773112596
135-139	12.52657823875536	14.86373458329178	21.05953924404109	51.550147933911774
140-144	39.75838408552964	19.53578362277599	18.687534905986546	22.01829738570783
145-149	0.6007818940985612	32.160314885242414	29.261056886784925	37.9778463338741
150-154	37.57785697188905	11.398500039892557	13.306295045344537	37.71734794287386
155-159	30.375123002047815	55.91752878912794	0.06356213930480573	13.643786069519429
160-164	29.30400787213106	11.192920401053163	0.23616393181032422	59.26690779500545
165-169	33.00922847796601	27.38650567804048	28.34033669317305	11.263929150820456
170-174	9.64189782186644	31.90247599797878	20.47538629291774	37.98023988723705
175-179	63.59591500226058	4.38046860455839	13.503896172974123	18.51972022020691
180-184	32.72093827291827	33.27331719901066	5.562219089917821	28.443525438153237
185-189	32.79779793090609	8.968245525384964	28.311614052817745	29.9223424908912
190-194	27.614691098640993	28.35616074040584	22.694476210739076	21.33467195021409
195-199	21.85460493071993	33.63766921092524	26.865774846413657	17.64195101194117
200-204	65.97364431797027	2.1165660487752986	25.542538762266958	6.367250870987473
205-209	24.00215419802665	44.50174197494747	12.484641365921119	19.01146246110476
210-214	47.99925533895375	27.17401132948592	24.339113321454217	0.48762001010611417
215-219	41.70992792744874	18.487939150554507	19.29350283237148	20.508630089625278
220-224	28.287013643254166	24.857450599718092	27.82585569532725	19.02968006170049
225-229	41.65359063419201	7.41291362371877	30.395525603314837	20.53797013877438
230-234	20.448005319148937	49.28191489361702	19.600398936170212	10.66968085106383
235-239	45.498936170212765	26.32912234042553	8.403058510638298	19.768882978723404
240-244	31.54760638297872	37.0156914893617	12.235106382978724	19.20159574468085
245-249	39.31968085106383	14.338829787234042	17.96875	28.372739361702127
250-254	37.05744680851063	1.6132978723404257	44.31742021276596	17.01183510638298
255-259	11.139494680851064	38.076462765957444	13.985239361702128	36.79880319148936
260-264	41.879654255319146	24.919015957446806	22.0469414893617	11.154388297872341
265-269	21.15026595744681	43.22672872340426	16.064760638297873	19.558244680851065
270-274	14.588962765957447	8.524867021276595	36.59082446808511	40.29534574468085
275-279	32.50997340425532	27.79015957446809	20.055851063829788	19.644015957446808
280-284	20.883244680851064	32.67406914893617	15.196808510638299	31.245877659574468
285-289	40.642420212765956	18.254787234042553	29.75970744680851	11.34308510638298
290-294	31.247240676812112	21.557029558667864	22.094000500002657	25.101729264517363
295-299	38.86835170594597	24.31436921435631	24.377687884185363	12.439591195512357
300-301	36.24602488679478	42.393310130573916	20.339231906352506	1.0214330762787935
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	1.6666666666666665
46	3.3333333333333335
47	0.25
48	3.25
49	123.41666666666666
50	993.1666666666669
51	529.9166666666666
52	280.58333333333337
53	1510.083333333333
54	15796.000000000013
55	24996.499999999956
56	4554.500000000003
57	1352.3333333333328
58	516.5833333333333
59	23.583333333333336
60	6.5
61	2.0
62	1.0
63	1.0
64	16.25
65	2.25
66	0.25
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	6.648405712310187E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	1.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	1.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	5.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	1.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	4.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	4.0
295-299	355.0
300-302	150041.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	3.6473153737733695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.79402114473204	1.9985107571204426
2	14.910681735326284	1.0876791745339467
3	7.746992344148743	0.847671728319549
4	4.393000364564346	0.6409063106667021
5	3.2081662413415963	0.5850597026832965
6	2.041560335399198	0.44677286386724463
7	1.8957345971563981	0.4840039358561816
8	1.4582573824279985	0.4254979655878521
9	1.3306598614655487	0.43680025529877936
>10	6.288734961720744	4.139962237055554
>50	0.5103900838497994	1.2572135201978565
>100	0.7109004739336493	5.760843549716778
>500	0.16405395552314983	4.886578198547988
>1k	0.40102078016769954	36.83815121133952
>5k	0.14582573824279985	40.16434858920831
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9295	6.1796931095923195	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8558	5.689705608595059	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8050	5.351966598409701	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	7898	5.250910831582586	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	7592	5.047469616785895	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	7163	4.762253011727788	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	6853	4.556152434646172	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	5003	3.326197377868787	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4135	2.7491157620402626	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3860	2.5662846049517327	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3624	2.4093822301412122	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3515	2.336914607877031	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3350	2.227215913623913	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	3289	2.1866606387788208	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	3262	2.1687099433555836	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3062	2.0357418291093796	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3052	2.0290934233970694	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	3048	2.0264340611121456	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2800	1.8615535994468528	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2720	1.808366353748371	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2623	1.7438768183389624	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2455	1.632183602372151	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2222	1.477275749275324	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1837	1.2213121293513816	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1253	0.8330452357524665	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1141	0.7585830917745925	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1066	0.708720048932266	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1041	0.6920990346514906	No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1035	0.6881099912241044	No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1019	0.6774725420844081	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	960	0.638246948381778	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	939	0.6242852963859267	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	875	0.5817354998271415	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	866	0.5757519346860622	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	799	0.531207616413584	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	781	0.5192404861314257	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	742	0.49331170385341594	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	730	0.4853336169986437	No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	658	0.43746509587001037	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	451	0.29984309762518946	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	438	0.2912001701991862	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	395	0.2626120256362524	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	379	0.25197457649655614	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	378	0.25130973592532513	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	356	0.2366832433582427	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	328	0.21806770736377415	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	323	0.21474350450761906	No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	273	0.18150147594606814	No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	269	0.17884211366114405	No Hit
CCTACGGGCGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	244	0.16222109938036858	No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	231	0.15357817195436532	No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	230	0.15291333138313434	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	230	0.15291333138313434	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	228	0.15158365024067227	No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	209	0.13895167938728295	No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	207	0.13762199824482088	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	207	0.13762199824482088	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	202	0.1342977953886658	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	191	0.12698454910512458	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	188	0.12499002739143153	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	181	0.12033614339281441	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	179	0.11900646225035236	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	179	0.11900646225035236	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	174	0.11568225939419727	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	173	0.11501741882296625	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	173	0.11501741882296625	No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	168	0.11169321596681116	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	159	0.10570965082573199	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	152	0.10105576682711485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
