##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IAFS2_cleaned_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	126693
Sequences flagged as poor quality	0
Sequence length	51-301
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.72434151847379	40.0	40.0	40.0	40.0	40.0
2	39.30987505229176	40.0	40.0	40.0	40.0	40.0
3	39.23405397298983	40.0	40.0	40.0	40.0	40.0
4	38.992714672475984	40.0	40.0	40.0	40.0	40.0
5	39.31276392539446	40.0	40.0	40.0	40.0	40.0
6	39.33344383667606	40.0	40.0	40.0	40.0	40.0
7	39.38538040775733	40.0	40.0	40.0	40.0	40.0
8	39.429392310545964	40.0	40.0	40.0	40.0	40.0
9	39.0463561522736	40.0	40.0	40.0	40.0	40.0
10-14	39.61876346759489	40.0	40.0	40.0	40.0	40.0
15-19	39.51946832105957	40.0	40.0	40.0	40.0	40.0
20-24	39.4757026828633	40.0	40.0	40.0	40.0	40.0
25-29	39.65238174169054	40.0	40.0	40.0	40.0	40.0
30-34	39.51803493484249	40.0	40.0	40.0	40.0	40.0
35-39	39.65692185045741	40.0	40.0	40.0	40.0	40.0
40-44	39.61116083761534	40.0	40.0	40.0	40.0	40.0
45-49	39.463623088884155	40.0	40.0	40.0	40.0	40.0
50-54	39.59219039422071	40.0	40.0	40.0	40.0	40.0
55-59	39.45869352445301	40.0	40.0	40.0	40.0	40.0
60-64	39.376143718624704	40.0	40.0	40.0	40.0	40.0
65-69	39.52339216367253	40.0	40.0	40.0	40.0	40.0
70-74	39.42957724244625	40.0	40.0	40.0	40.0	40.0
75-79	39.457228554289145	40.0	40.0	40.0	40.0	40.0
80-84	39.50965175385975	40.0	40.0	40.0	40.0	40.0
85-89	39.630278154895336	40.0	40.0	40.0	40.0	40.0
90-94	39.495437754554345	40.0	40.0	40.0	40.0	40.0
95-99	39.27320430650712	40.0	40.0	40.0	36.8	40.0
100-104	39.42355949862659	40.0	40.0	40.0	40.0	40.0
105-109	39.422031383197044	40.0	40.0	40.0	40.0	40.0
110-114	39.41771856155084	40.0	40.0	40.0	40.0	40.0
115-119	39.41180816468285	40.0	40.0	40.0	40.0	40.0
120-124	39.257190667129734	40.0	40.0	40.0	40.0	40.0
125-129	39.45201906987023	40.0	40.0	40.0	40.0	40.0
130-134	39.42850377292962	40.0	40.0	40.0	40.0	40.0
135-139	39.14150854039718	40.0	40.0	40.0	40.0	40.0
140-144	39.487159410223214	40.0	40.0	40.0	40.0	40.0
145-149	39.04634231048527	40.0	40.0	40.0	36.8	40.0
150-154	39.32558961891832	40.0	40.0	40.0	40.0	40.0
155-159	39.31707132257759	40.0	40.0	40.0	40.0	40.0
160-164	39.45850408865596	40.0	40.0	40.0	40.0	40.0
165-169	39.35145391974237	40.0	40.0	40.0	40.0	40.0
170-174	39.07050168913586	40.0	40.0	40.0	40.0	40.0
175-179	39.358046285479745	40.0	40.0	40.0	40.0	40.0
180-184	39.41796482808702	40.0	40.0	40.0	40.0	40.0
185-189	39.321554636441135	40.0	40.0	40.0	40.0	40.0
190-194	39.405108451993804	40.0	40.0	40.0	40.0	40.0
195-199	39.33309759100811	40.0	40.0	40.0	40.0	40.0
200-204	39.556379250465696	40.0	40.0	40.0	40.0	40.0
205-209	39.35514791778486	40.0	40.0	40.0	40.0	40.0
210-214	39.42835853881855	40.0	40.0	40.0	40.0	40.0
215-219	39.418091118618385	40.0	40.0	40.0	40.0	40.0
220-224	39.33602753133583	40.0	40.0	40.0	40.0	40.0
225-229	39.43914375019733	40.0	40.0	40.0	40.0	40.0
230-234	39.25970384870395	40.0	40.0	40.0	40.0	40.0
235-239	39.42167145518265	40.0	40.0	40.0	40.0	40.0
240-244	39.319723423736306	40.0	40.0	40.0	40.0	40.0
245-249	39.31184289457898	40.0	40.0	40.0	40.0	40.0
250-254	39.454064976478385	40.0	40.0	40.0	40.0	40.0
255-259	39.26203390900767	40.0	40.0	40.0	40.0	40.0
260-264	39.17332743977521	40.0	40.0	40.0	40.0	40.0
265-269	39.22856060366874	40.0	40.0	40.0	40.0	40.0
270-274	37.38887213772091	40.0	36.8	40.0	31.2	40.0
275-279	39.20143674145656	40.0	40.0	40.0	40.0	40.0
280-284	38.26259896281445	40.0	40.0	40.0	36.8	40.0
285-289	39.16231401305559	40.0	40.0	40.0	40.0	40.0
290-294	39.05577271778797	40.0	40.0	40.0	40.0	40.0
295-299	38.984237185389716	40.0	40.0	40.0	40.0	40.0
300-301	38.34561643346202	40.0	40.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.030795090153574733
1101	2	-0.23037812692228954
1101	3	-0.15644901190076155
1101	4	0.01972319661230415
1101	5	-0.008476706638440135
1101	6	-0.08428387402226178
1101	7	-0.19289150708605973
1101	8	-0.14307018600717925
1101	9	0.22544168791645802
1101	10-14	-0.11417782885816052
1101	15-19	-0.08512279515099408
1101	20-24	-0.21167990821442828
1101	25-29	-0.011201032754684093
1101	30-34	-0.205298385776004
1101	35-39	-0.1364561330030014
1101	40-44	-0.12672831010627306
1101	45-49	-0.10188635364881549
1101	50-54	-0.01024726882680227
1101	55-59	-0.019513993171194954
1101	60-64	-0.09366148853386136
1101	65-69	-0.10120214046922626
1101	70-74	-0.12808415820816776
1101	75-79	-0.08838537440567507
1101	80-84	-0.05039358013797823
1101	85-89	-0.07000917797285666
1101	90-94	-0.1510758309400586
1101	95-99	0.11189358029128016
1101	100-104	0.05362345497389498
1101	105-109	-0.07301325723071272
1101	110-114	-0.053327575531355365
1101	115-119	-0.0018754611586970782
1101	120-124	-0.04002824983327713
1101	125-129	0.021055025559711282
1101	130-134	0.004100466868209196
1101	135-139	0.12803888428879873
1101	140-144	0.029676559757284338
1101	145-149	0.20195306037173566
1101	150-154	0.07502065043125583
1101	155-159	0.07589337678099639
1101	160-164	0.06736875890078409
1101	165-169	0.14216905885008657
1101	170-174	0.10456446161751387
1101	175-179	0.07085410854666208
1101	180-184	-0.07973201878764513
1101	185-189	0.048780114838571365
1101	190-194	-0.09123250495504465
1101	195-199	0.06963089906513176
1101	200-204	0.02398849986060725
1101	205-209	-0.11975398786537284
1101	210-214	0.09691063511635178
1101	215-219	0.10596702668832592
1101	220-224	-0.025371233576883867
1101	225-229	0.07426994442920432
1101	230-234	0.09237924107464579
1101	235-239	-0.0033634316701309785
1101	240-244	0.02083704179324286
1101	245-249	-0.160163004734855
1101	250-254	0.12771146467432004
1101	255-259	-0.10244919856528156
1101	260-264	-0.04862318887768424
1101	265-269	-0.05351662870761942
1101	270-274	0.35486341702898727
1101	275-279	-0.09382232226373333
1101	280-284	-0.049006649716446304
1101	285-289	-0.04047827098320056
1101	290-294	-0.05764419794002151
1101	295-299	-0.16481462575568173
1101	300-301	0.006251181843538234
1102	1	0.023701682248514544
1102	2	-0.134026002878592
1102	3	-0.011670737736004355
1102	4	-0.11637019992511455
1102	5	-0.04003327770324461
1102	6	-0.11731823840062106
1102	7	0.024412315851570554
1102	8	0.15555773973938614
1102	9	-0.022441088970857948
1102	10-14	-1.1946738991497341E-4
1102	15-19	0.02287434020350787
1102	20-24	0.06333515390973332
1102	25-29	-0.016798459120792586
1102	30-34	-0.06433963894473749
1102	35-39	-0.08561094890405485
1102	40-44	-0.005571993676625198
1102	45-49	-0.01356887817450314
1102	50-54	-0.1297893802524257
1102	55-59	-0.09312628841156112
1102	60-64	-0.09129701923217937
1102	65-69	0.10546611766716296
1102	70-74	0.0535525547247957
1102	75-79	0.04894936851512455
1102	80-84	-0.0010261893525935761
1102	85-89	0.037837904522113774
1102	90-94	-0.003182823175954752
1102	95-99	0.08710757613071962
1102	100-104	-0.06699630880651597
1102	105-109	0.07452508013813741
1102	110-114	-0.09544241143945698
1102	115-119	0.041371591780396955
1102	120-124	-0.04730353999232051
1102	125-129	0.010433101927525001
1102	130-134	0.013367367708276845
1102	135-139	-0.12880614066317264
1102	140-144	-0.06329255436246228
1102	145-149	0.042715147015897514
1102	150-154	-0.1358991053918217
1102	155-159	-0.061418630858064205
1102	160-164	-0.1532089446084015
1102	165-169	-0.08680705773644348
1102	170-174	0.05736601671078745
1102	175-179	-0.12127266338435305
1102	180-184	-0.09097234208334726
1102	185-189	-0.13626234330008913
1102	190-194	-0.14791156235025227
1102	195-199	-0.15943161159202646
1102	200-204	-0.13429453166187244
1102	205-209	-0.10272071478053135
1102	210-214	-0.09285166751298846
1102	215-219	0.03307771340750776
1102	220-224	-0.04894317369215173
1102	225-229	0.04075813913416226
1102	230-234	-0.15389387786498787
1102	235-239	-0.14619101866100692
1102	240-244	-0.16280037593345043
1102	245-249	-0.33004557700463266
1102	250-254	-0.07008093946804905
1102	255-259	-0.05779255815785689
1102	260-264	-0.2239758699357779
1102	265-269	-0.25545594824063755
1102	270-274	0.0838815938867441
1102	275-279	-0.12836176229374274
1102	280-284	-0.08298225475913057
1102	285-289	-0.1914178065013914
1102	290-294	-0.268422995016266
1102	295-299	-0.04974557669895319
1102	300-301	0.13310655814333217
1103	1	0.1773294688394813
1103	2	0.07804713730676127
1103	3	0.06330630158937112
1103	4	0.03258195837830158
1103	5	0.006556018447710699
1103	6	-0.25875599409904027
1103	7	-0.22189310286764652
1103	8	-0.4222163272222872
1103	9	-0.050327871971610705
1103	10-14	-0.140755686810472
1103	15-19	-0.012660434781885499
1103	20-24	-0.24221715468173244
1103	25-29	-0.11575479209231787
1103	30-34	-0.024563401155646147
1103	35-39	-0.057490384488936286
1103	40-44	-0.12476711766426263
1103	45-49	-0.048702885020965425
1103	50-54	-0.10547611315715955
1103	55-59	-0.03892239762087257
1103	60-64	-0.16089708602686414
1103	65-69	-0.12547652239294393
1103	70-74	-0.17986148885874087
1103	75-79	-0.02048371155501627
1103	80-84	-0.15502134673757695
1103	85-89	-0.11388623340892678
1103	90-94	-0.10651420833083591
1103	95-99	-0.08923040499978185
1103	100-104	-0.19804749023759172
1103	105-109	-0.1511741767709367
1103	110-114	-0.08786121414463821
1103	115-119	-0.135634336248323
1103	120-124	-0.11416502450429533
1103	125-129	-7.216112727661539E-4
1103	130-134	-0.06718702821725486
1103	135-139	-0.01745939272963426
1103	140-144	-0.008353921983733414
1103	145-149	-0.03997667898394042
1103	150-154	-0.1283513054419103
1103	155-159	-0.07033572194932702
1103	160-164	-0.021523213624021764
1103	165-169	-0.018676474117832242
1103	170-174	0.13892233298363976
1103	175-179	-0.1264492518455782
1103	180-184	-0.12233244895484319
1103	185-189	-0.09742123116850365
1103	190-194	-0.015023526949065058
1103	195-199	-0.03238811155028998
1103	200-204	0.015459162919412961
1103	205-209	-0.15675761884666173
1103	210-214	0.021534238807461747
1103	215-219	-0.01371196681291309
1103	220-224	-0.10652019631885423
1103	225-229	-0.025769371928703322
1103	230-234	-0.03005652293470007
1103	235-239	-0.20233195545653615
1103	240-244	-0.13407865930992102
1103	245-249	-0.12485228987095809
1103	250-254	0.011575568839539585
1103	255-259	-0.040292349424461804
1103	260-264	-0.16162302698679554
1103	265-269	-0.04154595408517281
1103	270-274	-0.1451573975134437
1103	275-279	-0.012673359521762961
1103	280-284	-0.12082144654338123
1103	285-289	-0.14088762365093288
1103	290-294	-0.2071388671044332
1103	295-299	-0.06494136863349098
1103	300-301	-0.5619935687715412
1104	1	-0.12999141103175305
1104	2	0.2729454448715103
1104	3	0.25776564900194643
1104	4	0.05770023375263378
1104	5	0.022992637479070765
1104	6	0.08324273779385294
1104	7	-0.01802049885847623
1104	8	0.20676479749116794
1104	9	-0.15465406813802218
1104	10-14	-0.054251272822277485
1104	15-19	0.06408930915803523
1104	20-24	0.049018274279880814
1104	25-29	0.13143830160128545
1104	30-34	-0.0508522369357749
1104	35-39	0.02172729097318893
1104	40-44	-0.06396625925330568
1104	45-49	0.06720210449277175
1104	50-54	0.040522033455538065
1104	55-59	0.07111028052377577
1104	60-64	-0.11531351289122682
1104	65-69	-0.027442088407688914
1104	70-74	0.05804515894890727
1104	75-79	-0.009363387699963255
1104	80-84	0.026025329358603244
1104	85-89	0.024259403814902214
1104	90-94	0.07858065583538121
1104	95-99	0.04144315496705531
1104	100-104	-0.09884249584652594
1104	105-109	0.08078212230724802
1104	110-114	0.14829934772435394
1104	115-119	-0.06597623743351022
1104	120-124	0.014255206681376364
1104	125-129	-0.0846020190179857
1104	130-134	-0.1871792244812127
1104	135-139	-0.20191044867267038
1104	140-144	-0.016918522286132998
1104	145-149	-0.06469501288629687
1104	150-154	-0.035340526531513206
1104	155-159	-0.03248760216197866
1104	160-164	-0.10356974340015057
1104	165-169	-0.053656720910986166
1104	170-174	-0.09072210929309676
1104	175-179	-0.13418470519386716
1104	180-184	-0.014943286393275912
1104	185-189	-0.024124640425689847
1104	190-194	-0.029957926792988587
1104	195-199	-0.1290861557389391
1104	200-204	-0.04603427706497598
1104	205-209	-0.0834317640936959
1104	210-214	-0.1357207970409462
1104	215-219	-0.008707821078147049
1104	220-224	-0.147948280013928
1104	225-229	-0.06047648796049998
1104	230-234	-0.11920445250598277
1104	235-239	-0.12311427999441094
1104	240-244	-0.1658593743272334
1104	245-249	-0.30706952637293483
1104	250-254	-0.14364074096877744
1104	255-259	-0.1531441272130678
1104	260-264	-0.19433043565869212
1104	265-269	-0.2438480562165637
1104	270-274	-0.14303087944291804
1104	275-279	-0.1771858698861024
1104	280-284	-0.1638395414563263
1104	285-289	-0.06078227321440721
1104	290-294	-0.13973409707077877
1104	295-299	-0.038828116914231714
1104	300-301	-0.49126678175180416
1201	1	-0.23660642035877544
1201	2	-0.17063804391215598
1201	3	-0.39622583710318793
1201	4	0.1760816371103644
1201	5	0.1839435151755353
1201	6	-0.04781384612540762
1201	7	-0.15778230112565694
1201	8	0.1467848864565866
1201	9	-0.17450387920608534
1201	10-14	0.027864764868446912
1201	15-19	0.03364974346233396
1201	20-24	0.126293827922197
1201	25-29	0.030467334939402235
1201	30-34	0.020080943338768975
1201	35-39	0.06614303689561751
1201	40-44	0.028242092016611764
1201	45-49	0.06312691436505702
1201	50-54	-0.03574907191587329
1201	55-59	0.13834135145695825
1201	60-64	0.08242887004271893
1201	65-69	0.07929728118145363
1201	70-74	0.08183623370855742
1201	75-79	0.05205144943592188
1201	80-84	0.1179945262782951
1201	85-89	0.06571920196862635
1201	90-94	0.011569606904394902
1201	95-99	0.06543511938087931
1201	100-104	0.0697900195789245
1201	105-109	-0.028568812786268438
1201	110-114	0.06471011923629533
1201	115-119	0.10067607997214623
1201	120-124	-0.0241338496155592
1201	125-129	0.08708210591625232
1201	130-134	0.14611584415608547
1201	135-139	-0.09747652701963005
1201	140-144	0.05213012170870002
1201	145-149	0.164720972190473
1201	150-154	0.1636645913715853
1201	155-159	0.0988368346295303
1201	160-164	0.041730873618071485
1201	165-169	-0.01160381996871962
1201	170-174	-0.03908390199410405
1201	175-179	-0.04214854505611498
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2404	210-214	0.04593225877557927
2404	215-219	-0.08634989185456021
2404	220-224	0.009884583564399918
2404	225-229	0.0322700129669613
2404	230-234	-0.05331830184712061
2404	235-239	0.03511365958787138
2404	240-244	0.008253511413890635
2404	245-249	-0.050790467810138296
2404	250-254	-0.053707952069459
2404	255-259	-0.10457386872736407
2404	260-264	0.10561415734074586
2404	265-269	1.2101222312566051E-4
2404	270-274	-0.05111307452374092
2404	275-279	0.12201675110592447
2404	280-284	0.017008908352153185
2404	285-289	-0.005866708665251963
2404	290-294	0.009700992423567811
2404	295-299	-0.16473780526601445
2404	300-301	0.29537812959164
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	22.0
22	27.0
23	70.0
24	172.0
25	228.0
26	227.0
27	242.0
28	274.0
29	297.0
30	399.0
31	419.0
32	558.0
33	642.0
34	780.0
35	1064.0
36	1531.0
37	2421.0
38	4850.0
39	102561.0
40	9905.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.057619600135761256	0.009471715090810068	0.10971403313521663	99.82319465163822
2	0.008682474031509489	0.011839737315694756	0.15075932181984655	99.82871846683294
3	0.005525167136305873	0.13260401127134097	99.61165968127679	0.250211140315566
4	0.08050957827188558	99.39933540132446	0.10182093722620825	0.4183340831774447
5	0.1957487785434081	0.13260401127134097	0.09866369886260488	99.57298351132265
6	99.77267883782056	0.07340579195377803	0.01657550140891762	0.13733986881674598
7	99.90370422991009	0.05840890972666209	0.030783074045132724	0.0071037863181075515
8	99.74584231172993	0.11287127149881998	0.08366681663548893	0.057619600135761256
9	29.93456623491432	21.29162621455013	26.204289108316953	22.56951844221859
10-14	59.82808837110179	10.315171319646705	9.703456386698555	20.15328392255294
15-19	39.67906672033972	20.36702895976889	19.940328194927897	20.013576124963496
20-24	59.92296338392807	39.835665743174445	0.21248214187050588	0.02888873102697071
25-29	19.400440434751722	19.572983511322647	59.704640351084905	1.3219357028407253
30-34	0.506105309685618	59.55956524827734	0.2318991578066665	39.70243028423038
35-39	59.750578169275336	0.6353942206751754	19.83171919521994	19.78230841482955
40-44	39.65333522767635	41.74895219151808	0.07135358701743585	18.526358993788133
45-49	20.107188242444334	19.92627848420986	20.63160553463885	39.33492773870695
50-54	40.39737190233984	20.053452298638277	19.67158251007953	19.877593288942354
55-59	20.09408644586872	21.829318346856944	18.1526852524232	39.92390995485113
60-64	39.97489975689072	0.06456603416158874	0.32204085498689733	59.63849335396078
65-69	22.184668329492	20.1158715625296	56.34357339058504	1.3558867173933633
70-74	40.0255738326019	58.5208221513592	1.3560445805575727	0.09755943548132479
75-79	40.38060808890853	19.87370946863259	1.0780475483850598	38.667634894073814
80-84	40.060145865563726	0.14397120575884823	39.699112809017144	20.096770119660277
85-89	39.16269377703407	0.5654658541975816	58.002873109588606	2.268967259179743
90-94	20.035519211947083	59.69406118776245	18.23414264515518	2.0362769551352886
95-99	1.89246361254065	17.88715941022322	59.81624727686041	20.404129700375716
100-104	37.41025479114703	20.07893158210463	2.5685915448489247	39.94222208189941
105-109	76.45139393173996	22.977772866479334	0.4448583967417043	0.1259748050389922
110-114	56.92498342436776	41.60736273797872	1.2636946294951537	0.20395920815836832
115-119	39.41132826066366	39.240046727496605	19.83771666719288	1.51090834464686
120-124	21.203075174438794	38.04896915353771	39.11344046980078	1.6345152022227134
125-129	4.028983676948821	56.19739210052727	20.582515075932182	19.191109146591735
130-134	15.951125564360813	10.16102042749345	54.67653837653522	19.21131563161052
135-139	1.6184131594733684	19.036403245666655	38.00681968869384	41.33836390616614
140-144	39.865026994601074	19.80677548700786	20.047832538755404	20.28036497963565
145-149	0.6608152053799766	21.92245761374041	38.314337132573485	39.10239004830613
150-154	38.932371420452746	18.82486660562624	2.8660057462191775	39.376756227701826
155-159	21.7364948063019	76.38572285542892	0.05556783380166072	1.8222145044675275
160-164	21.57721087361475	18.641745335143497	0.47643102958355693	59.30461276165819
165-169	38.45830833833233	21.507119628705837	21.21949294351656	18.81507908944527
170-174	1.7454930066618257	21.66735074037824	20.076247908313075	56.51090834464686
175-179	43.5872825434913	0.5926183184415748	18.719256148770246	37.10084298929688
180-184	38.72730717014492	38.396110251633885	1.3037918732043066	21.572790705016892
185-189	38.89353708205727	1.426925141287532	4.20484324187794	55.47469453477726
190-194	21.281059577558175	38.53676633094434	37.31001168187415	2.8721624096233382
195-199	37.088056072995926	39.788463359959586	3.3405424178322227	19.782938149212264
200-204	78.00318883591703	0.2693145581410034	20.07056483440154	1.6569317715404288
205-209	20.51684399962113	40.96501752281122	1.5738957471663564	36.944242730401285
210-214	24.238625959018723	36.47965143813342	38.916742967196036	0.364979635651817
215-219	39.93795977646576	20.1567581220598	19.44747892526758	20.457803176206866
220-224	4.570927919679222	36.50033151264484	40.599248571338364	18.329491996337573
225-229	56.473968364221896	0.31935718119533985	23.237299908439365	19.969374546143403
230-234	20.14949641650617	58.209200265210114	20.10071669876551	1.5405866195182016
235-239	41.23133268083225	21.128405897767816	0.6167713825655922	37.02349003883434
240-244	38.79187320430651	22.643734411012534	2.0386449025984277	36.52574748208253
245-249	57.77681305844094	1.8642061061471915	20.281627884949327	20.07735295046254
250-254	21.09525463328387	1.3811448236668455	74.21068417895368	3.312916364095602
255-259	18.690998642376787	39.80677548700786	18.941843210305308	22.560382660310044
260-264	39.83329649859502	37.608530925393865	21.933508035235057	0.6246645407760553
265-269	20.435228743724938	58.19562403308812	18.653111482966565	2.716035740220377
270-274	2.292664830177361	2.1085949278164984	22.5155693774617	73.08317086454444
275-279	56.03894248453304	3.4779547460166356	19.697306957014558	20.78579581243577
280-284	2.760302788718831	40.784756371902844	1.1195920719241608	55.335348767454164
285-289	56.91464925920956	20.874898373181573	20.522697313894657	1.6877550537142134
290-294	21.681232910449175	20.05342359352602	20.454258416110434	37.81108507991437
295-299	22.68207449891121	21.70382032336867	36.70956036199352	18.904544815726602
300-301	6.857240426309186	48.22344700519386	44.90978255690211	0.00953001159484744
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.3333333333333333
47	0.5833333333333333
48	2.0
49	13.583333333333334
50	683.0833333333334
51	508.4166666666666
52	30.833333333333332
53	206.08333333333331
54	16091.250000000013
55	25265.083333333256
56	1155.4166666666677
57	85.83333333333333
58	18.583333333333336
59	4.5
60	2.833333333333333
61	0.75
62	0.0
63	0.5833333333333333
64	1.0
65	0.0
66	0.0
67	0.25
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.89309590900839E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	1.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	1.0
270-274	0.0
275-279	2.0
280-284	0.0
285-289	1.0
290-294	3.0
295-299	433.0
300-302	126252.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	2.2519002628400937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.50192779530319	1.2723670605321524
2	12.86365229582895	0.5793532397212159
3	5.222572730459166	0.352821387132675
4	3.399929898352611	0.30625212126952556
5	2.173151069050123	0.24468597317926008
6	1.8927444794952681	0.25573630745187187
7	1.4370837714686295	0.22653185258854083
8	1.4721345951629863	0.26520802254268194
9	1.2267788293024886	0.24863252113376427
>10	12.372940764107957	5.780903443757745
>50	0.3505082369435682	0.558831190357794
>100	0.5257623554153523	2.509215189473767
>500	0.24535576586049773	4.5424766956343285
>1k	0.03505082369435682	0.8169354265823684
>5k	0.03505082369435682	7.031169835744675
>10k+	0.24535576586049773	75.00887973289764
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	16379	12.928101789364844	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	14487	11.434728043380456	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	14357	11.332117796563345	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	13220	10.434672791709092	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	12932	10.20735162952965	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	12536	9.894785031532919	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	11120	8.777122650817331	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8908	7.031169835744675	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1035	0.8169354265823684	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	970	0.7656303031738139	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	940	0.7419510154467888	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	886	0.6993282975381434	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	845	0.666966604311209	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	800	0.6314476727206713	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	744	0.5872463356302242	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	570	0.44990646681347823	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	338	0.2667866417244836	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	334	0.26362940336088025	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	320	0.2525790690882685	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	311	0.24547528277016092	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	311	0.24547528277016092	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	263	0.2075884224069207	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	245	0.19338084977070558	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	193	0.15233675104386193	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	179	0.1412864167712502	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	140	0.11050334272611746	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
