##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IAFS1_cleaned_R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	162981
Sequences flagged as poor quality	0
Sequence length	51-301
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.874985427749245	40.0	40.0	40.0	40.0	40.0
2	39.30418883182702	40.0	40.0	40.0	40.0	40.0
3	39.29815131825182	40.0	40.0	40.0	40.0	40.0
4	39.51677802934084	40.0	40.0	40.0	40.0	40.0
5	38.790705665077525	40.0	40.0	40.0	40.0	40.0
6	38.01000116578006	40.0	40.0	40.0	24.0	40.0
7	38.857584626428846	40.0	40.0	40.0	40.0	40.0
8	38.959510617802074	40.0	40.0	40.0	40.0	40.0
9	39.37980500794571	40.0	40.0	40.0	40.0	40.0
10-14	39.25223185524693	40.0	40.0	40.0	40.0	40.0
15-19	39.1381424828661	40.0	40.0	40.0	40.0	40.0
20-24	39.19943060847583	40.0	40.0	40.0	40.0	40.0
25-29	39.301037544253624	40.0	40.0	40.0	40.0	40.0
30-34	39.26566655008866	40.0	40.0	40.0	40.0	40.0
35-39	39.44005252145955	40.0	40.0	40.0	40.0	40.0
40-44	39.37177462403593	40.0	40.0	40.0	40.0	40.0
45-49	39.0671403415122	40.0	40.0	40.0	40.0	40.0
50-54	39.38435314038388	40.0	40.0	40.0	40.0	40.0
55-59	39.13951650509265	40.0	40.0	40.0	40.0	40.0
60-64	39.174215241133886	40.0	40.0	40.0	40.0	40.0
65-69	39.1943845870659	40.0	40.0	40.0	40.0	40.0
70-74	37.96309240397595	40.0	36.8	40.0	36.8	40.0
75-79	39.180895815437474	40.0	40.0	40.0	40.0	40.0
80-84	38.992730396367655	40.0	40.0	40.0	40.0	40.0
85-89	39.17169223217572	40.0	40.0	40.0	40.0	40.0
90-94	39.125797030310466	40.0	40.0	40.0	40.0	40.0
95-99	38.03731991655418	40.0	36.8	40.0	36.8	40.0
100-104	38.99033927391415	40.0	40.0	40.0	36.8	40.0
105-109	38.98673080580933	40.0	40.0	40.0	40.0	40.0
110-114	39.274198516373275	40.0	40.0	40.0	40.0	40.0
115-119	39.27151841648311	40.0	40.0	40.0	40.0	40.0
120-124	38.768695353389084	40.0	40.0	40.0	40.0	40.0
125-129	38.80405205578633	40.0	40.0	40.0	36.8	40.0
130-134	39.062662060756296	40.0	40.0	40.0	40.0	40.0
135-139	38.21925033286497	40.0	40.0	40.0	36.8	40.0
140-144	37.97182704520215	40.0	36.8	40.0	36.8	40.0
145-149	38.72007068395315	40.0	40.0	40.0	40.0	40.0
150-154	38.99679099761319	40.0	40.0	40.0	40.0	40.0
155-159	39.16710094537716	40.0	40.0	40.0	40.0	40.0
160-164	38.420243223011695	40.0	40.0	40.0	36.8	40.0
165-169	39.06593037097032	40.0	40.0	40.0	40.0	40.0
170-174	38.97843880769184	40.0	40.0	40.0	40.0	40.0
175-179	39.01441912405356	40.0	40.0	40.0	40.0	40.0
180-184	39.0028052866031	40.0	40.0	40.0	40.0	40.0
185-189	38.722134275791824	40.0	40.0	40.0	36.8	40.0
190-194	38.36943391132546	40.0	40.0	40.0	36.8	40.0
195-199	38.66098737252881	40.0	40.0	40.0	36.8	40.0
200-204	37.75278135699297	40.0	40.0	40.0	33.6	40.0
205-209	38.69002687479291	40.0	40.0	40.0	40.0	40.0
210-214	37.98275840911043	40.0	40.0	40.0	36.8	40.0
215-219	38.64914282909349	40.0	40.0	40.0	40.0	40.0
220-224	37.977655879934716	40.0	40.0	40.0	33.6	40.0
225-229	38.422010332682945	40.0	40.0	40.0	36.8	40.0
230-234	38.662877198149445	40.0	40.0	40.0	36.8	40.0
235-239	38.304394458147726	40.0	40.0	40.0	33.6	40.0
240-244	38.129455509332544	40.0	40.0	40.0	33.6	40.0
245-249	37.189706586165	40.0	40.0	40.0	28.0	40.0
250-254	38.48773699517726	40.0	40.0	40.0	36.8	40.0
255-259	38.585271631753976	40.0	40.0	40.0	40.0	40.0
260-264	37.64708856410068	40.0	40.0	40.0	30.4	40.0
265-269	37.7383646872584	40.0	40.0	40.0	33.6	40.0
270-274	38.38680558112138	40.0	40.0	40.0	36.8	40.0
275-279	37.566130397967825	40.0	40.0	40.0	30.4	40.0
280-284	38.22992060278074	40.0	40.0	40.0	33.6	40.0
285-289	38.17938126618317	40.0	40.0	40.0	33.6	40.0
290-294	37.8348537839463	40.0	40.0	40.0	27.2	40.0
295-299	38.07817605581392	40.0	40.0	40.0	30.4	40.0
300-301	35.44165518689731	40.0	32.0	40.0	18.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.04795989074683149
1101	2	0.052866483410859644
1101	3	-0.1746863105212526
1101	4	-0.09121223717582438
1101	5	-0.037756900404644966
1101	6	0.5611153302631422
1101	7	-0.0557186913664367
1101	8	0.03454130406247913
1101	9	0.07198041913544273
1101	10-14	0.11607169890839941
1101	15-19	0.10288713512072434
1101	20-24	0.10841350873705125
1101	25-29	-0.005078900269857911
1101	30-34	0.07308502377611603
1101	35-39	-0.01703888848438595
1101	40-44	0.043601670763713685
1101	45-49	0.03448879266881022
1101	50-54	0.007479709331036588
1101	55-59	-0.049081916419048355
1101	60-64	0.016076533986492336
1101	65-69	-0.09236074569397346
1101	70-74	-0.3455749810272408
1101	75-79	-0.17542920848060817
1101	80-84	-0.12129951588824639
1101	85-89	-0.16001445021287708
1101	90-94	-0.04726705283560051
1101	95-99	0.033731821022669806
1101	100-104	-0.055469944411278505
1101	105-109	0.054743221875938275
1101	110-114	-0.047688534401153504
1101	115-119	0.04463599738791402
1101	120-124	-0.16789103858121734
1101	125-129	-0.2215597042697368
1101	130-134	-0.3139147341452073
1101	135-139	-0.07725171054692481
1101	140-144	0.05874396529930692
1101	145-149	-0.04388552076208185
1101	150-154	-5.158031490921644E-4
1101	155-159	0.12367389203243562
1101	160-164	-0.23403543491470202
1101	165-169	-0.06320589964090573
1101	170-174	0.05676940362787519
1101	175-179	0.17589289076047976
1101	180-184	-0.06893308437858536
1101	185-189	0.05137430288613132
1101	190-194	-0.11703076333848372
1101	195-199	-0.03725707594458072
1101	200-204	-0.12155550600385823
1101	205-209	0.01070854922071618
1101	210-214	0.17164237014455352
1101	215-219	-0.40160555967768374
1101	220-224	-0.24922653137751638
1101	225-229	-0.29207028838446547
1101	230-234	-0.017831163651749193
1101	235-239	-0.046275029105338206
1101	240-244	-0.1054758346978204
1101	245-249	0.5753271487047869
1101	250-254	-0.11826906765293188
1101	255-259	-0.2779879207637208
1101	260-264	-0.3158587524240062
1101	265-269	-0.19429606007351907
1101	270-274	-0.04875110502140956
1101	275-279	-0.05970374014400193
1101	280-284	-0.2868896986856484
1101	285-289	-0.421051597714424
1101	290-294	-0.40598543402814613
1101	295-299	-0.4306610175644181
1101	300-301	-0.6422509117848918
1102	1	-0.19789201225151487
1102	2	0.019137694676103933
1102	3	0.04501113471131646
1102	4	0.10036393506697294
1102	5	-0.13463301196910038
1102	6	-0.46593884084978043
1102	7	0.05002901227216938
1102	8	-0.18076219226544055
1102	9	-0.08080346134914151
1102	10-14	-0.00583736727284645
1102	15-19	-0.04965403199579299
1102	20-24	0.019136836421019154
1102	25-29	-0.019532899809540538
1102	30-34	-0.010090336632316621
1102	35-39	-0.08755967604741954
1102	40-44	-0.12301687170066344
1102	45-49	-0.1502997746946093
1102	50-54	-0.2146365001557058
1102	55-59	-0.17704207794897542
1102	60-64	-0.13967849290029477
1102	65-69	0.04328254934923592
1102	70-74	-0.22405090846432074
1102	75-79	-0.09400305820754795
1102	80-84	-0.2354752319972988
1102	85-89	-0.17759024583148175
1102	90-94	-0.13184847689351642
1102	95-99	-0.10905059481228108
1102	100-104	-0.2397479767591122
1102	105-109	-0.10782451816967864
1102	110-114	0.0017831455519825568
1102	115-119	0.07237227675936708
1102	120-124	-0.0378468898565103
1102	125-129	-0.3312912549152358
1102	130-134	-0.14400700707219727
1102	135-139	-0.4561942335105371
1102	140-144	-0.3254294492174594
1102	145-149	-0.368828504044167
1102	150-154	-0.1199391496642761
1102	155-159	-0.1903218814071863
1102	160-164	-0.3926528056593668
1102	165-169	-0.14359424102981677
1102	170-174	-0.20030590028692075
1102	175-179	-0.13277992743466172
1102	180-184	-0.05241183649708603
1102	185-189	-0.4092789308510234
1102	190-194	-0.3500104884028232
1102	195-199	-0.4629344764007044
1102	200-204	-0.426771751060663
1102	205-209	-0.3141591092229845
1102	210-214	0.14495226908373837
1102	215-219	-0.327435702167179
1102	220-224	-0.39818558395843695
1102	225-229	-0.46276474154706193
1102	230-234	-0.4208608960393363
1102	235-239	-0.47542574155276895
1102	240-244	-0.5478753492710808
1102	245-249	0.09096332973548016
1102	250-254	-0.5378367867749816
1102	255-259	-0.7068540727106551
1102	260-264	-0.6527867544326682
1102	265-269	-0.7920070219298623
1102	270-274	-0.7424844969407332
1102	275-279	-0.8345703524094006
1102	280-284	-0.7581721040588292
1102	285-289	-0.6326474381094584
1102	290-294	-0.7826933201202593
1102	295-299	-0.9324959206336558
1102	300-301	-1.3398354329618982
1103	1	-0.07611566126168157
1103	2	-0.03102816942234199
1103	3	0.14012081903447893
1103	4	-0.08891375353655917
1103	5	0.17000048187787797
1103	6	-0.11276602116010537
1103	7	-0.10547660570535555
1103	8	-0.27762682575928466
1103	9	-0.031536473655194186
1103	10-14	-0.22754309225947367
1103	15-19	-0.05754702290002456
1103	20-24	-0.06129119996207777
1103	25-29	-0.08826698220654094
1103	30-34	-0.07331987114275762
1103	35-39	-0.08300536893131749
1103	40-44	-0.06591681819612205
1103	45-49	-0.08190812140407644
1103	50-54	-0.12212071631407895
1103	55-59	-0.057696973615108504
1103	60-64	-0.011147727785257189
1103	65-69	-0.06924821576579632
1103	70-74	-0.22447894484741227
1103	75-79	-0.04833157613158079
1103	80-84	-0.06011728657193771
1103	85-89	-0.13552283010114508
1103	90-94	-0.28929738005029293
1103	95-99	-0.4268076841648849
1103	100-104	-0.27798018649694
1103	105-109	-0.12362762143345662
1103	110-114	-0.13745708986802896
1103	115-119	-0.18994554025506716
1103	120-124	-0.18584474967458675
1103	125-129	-0.24896273699120286
1103	130-134	-0.37691872456818487
1103	135-139	-0.7306254801665091
1103	140-144	-0.45895967928701964
1103	145-149	-0.47908211560114466
1103	150-154	-0.26087440916133176
1103	155-159	-0.19231653095240375
1103	160-164	-0.5822386804587296
1103	165-169	-0.32017637051082204
1103	170-174	-0.38573126144261494
1103	175-179	-0.5694883276486777
1103	180-184	-0.46952473109312365
1103	185-189	-0.6083500918997089
1103	190-194	-0.5224492256954889
1103	195-199	-0.35506074713372016
1103	200-204	-0.7279210287492646
1103	205-209	-0.504943485895005
1103	210-214	-0.3666624981714861
1103	215-219	-0.5402211489301578
1103	220-224	-0.5398485053067503
1103	225-229	-0.8128385778231433
1103	230-234	-0.6853959415182018
1103	235-239	-0.7698291658438379
1103	240-244	-0.8052193835139931
1103	245-249	0.16503930619347784
1103	250-254	-0.6199769686904375
1103	255-259	-0.7565747789706947
1103	260-264	-0.8668648710725861
1103	265-269	-0.7167924686928373
1103	270-274	-0.8141121052874425
1103	275-279	-0.4816372330942613
1103	280-284	-1.0325263041658346
1103	285-289	-1.0716586740645155
1103	290-294	-0.7637912334428876
1103	295-299	-1.072953572607517
1103	300-301	-1.5908226909753083
1104	1	-0.40184416161704206
1104	2	-0.2543543954351364
1104	3	-0.09426439773879736
1104	4	-0.27883557301061757
1104	5	0.04947454015790953
1104	6	-0.9267247987009739
1104	7	0.23313035946877392
1104	8	-0.16879953364841072
1104	9	-0.46631970037871895
1104	10-14	-0.4522737248110005
1104	15-19	-0.1373224315709507
1104	20-24	-0.37795075930819877
1104	25-29	-0.5541575294702241
1104	30-34	-0.4326460971555477
1104	35-39	-0.32702526942883026
1104	40-44	-0.3007853327661181
1104	45-49	-0.40598203753769724
1104	50-54	-0.3264817681975174
1104	55-59	-0.26122789045945893
1104	60-64	-0.4092799239473024
1104	65-69	-0.3403498504495204
1104	70-74	-0.3932778076761281
1104	75-79	-0.47425908146207263
1104	80-84	-0.5607086156408769
1104	85-89	-0.281274073882237
1104	90-94	-0.5310770531135347
1104	95-99	-0.7102021404548537
1104	100-104	-0.5395523258004218
1104	105-109	-0.46491546791532556
1104	110-114	-0.47155801381970264
1104	115-119	-0.32077993968648855
1104	120-124	-0.5242072230221453
1104	125-129	-0.7689456794219041
1104	130-134	-0.37291303871175074
1104	135-139	-0.8717512797400673
1104	140-144	-0.7733136238499227
1104	145-149	-0.5194573821256583
1104	150-154	-0.6501252975764018
1104	155-159	-0.5791082864605812
1104	160-164	-0.9329792632590213
1104	165-169	-0.2889070030623486
1104	170-174	-0.4718862010303866
1104	175-179	-0.34203701279437837
1104	180-184	-0.48284953564181166
1104	185-189	-0.5866784501086642
1104	190-194	-0.6128088561148246
1104	195-199	-0.3547394962453012
1104	200-204	-0.51694448290705
1104	205-209	-0.49319650650623004
1104	210-214	-0.3138380489888277
1104	215-219	-0.8018501467979533
1104	220-224	-0.27282362968486495
1104	225-229	-0.8134327498203007
1104	230-234	-0.5725686494073301
1104	235-239	-0.8047118950549219
1104	240-244	-0.6314965690150558
1104	245-249	0.15029588046497366
1104	250-254	-0.3630757604459518
1104	255-259	-0.5527481975918747
1104	260-264	-0.7372017580661847
1104	265-269	-0.9763717153168585
1104	270-274	-0.5696799801985932
1104	275-279	-0.7807104384958237
1104	280-284	-0.9936464178829638
1104	285-289	-0.9326138979451102
1104	290-294	-0.8748672817726657
1104	295-299	-1.0936005100461514
1104	300-301	-1.9563638405518233
1201	1	-0.022782327928347
1201	2	0.1442099258157512
1201	3	0.21631129522495485
1201	4	-0.08129470591750732
1201	5	-0.12714237526498096
1201	6	0.275805407411319
1201	7	-0.273095653324404
1201	8	-0.2700077781402399
1201	9	-0.008679330798045726
1201	10-14	-0.027924044640428747
1201	15-19	-0.02859464194764172
1201	20-24	0.03166118099030513
1201	25-29	0.04125682731726954
1201	30-34	-0.0047484425713193446
1201	35-39	-0.04033870226464842
1201	40-44	0.008749848470550603
1201	45-49	0.09028235478639601
1201	50-54	0.016545950352593763
1201	55-59	-0.022649354567491287
1201	60-64	0.09399512935760157
1201	65-69	0.014561308043724353
1201	70-74	0.07723534086687067
1201	75-79	0.05071604291604359
1201	80-84	0.020644618189962216
1201	85-89	-0.02580854438686231
1201	90-94	0.02155976280685934
1201	95-99	0.07909707773987407
1201	100-104	0.03744838493163627
1201	105-109	-0.24248476429060162
1201	110-114	-0.03993328034421495
1201	115-119	-0.05280268311221192
1201	120-124	-0.10965427348412504
1201	125-129	0.15789440586593173
1201	130-134	-0.07368062933009867
1201	135-139	0.05870785316682969
1201	140-144	-0.03838825071559171
1201	145-149	0.10758455106552844
1201	150-154	-0.0886839329708522
1201	155-159	-0.001840340476213953
1201	160-164	-0.16471487093492243
1201	165-169	0.11563315329869539
1201	170-174	-0.02306459477594558
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2404	295-299	0.8342003588794995
2404	300-301	1.0091121869274318
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	89.0
21	172.0
22	185.0
23	212.0
24	286.0
25	336.0
26	370.0
27	479.0
28	525.0
29	645.0
30	785.0
31	871.0
32	1054.0
33	1399.0
34	1845.0
35	2615.0
36	4056.0
37	7409.0
38	23779.0
39	115289.0
40	573.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	99.76623041949675	0.20063688405396948	0.01902062203569741	0.01411207441358195
2	0.2515630656334174	99.50116884790252	0.021474895846755142	0.2257931906173112
3	0.10185236315889581	0.08774028874531388	0.1877519465459164	99.62265540154988
4	0.09878452089507365	0.12700866972223757	99.58215988366742	0.19204692571526744
5	0.1521649762855793	98.92993661837883	0.08037746731214067	0.8375209380234505
6	0.10430663696995356	0.45281351814015125	0.12762223817500198	99.3152576067149
7	0.24910879182235965	31.474834489909863	37.68844221105528	30.587614507212496
8	34.16165074456532	33.63520901209343	0.08896742565084273	32.11417281769041
9	99.7600947349691	0.1693448929629834	0.026383443468870605	0.044176928599039156
10-14	39.977175253557164	19.855688699909805	39.86341966241464	0.3037163841183942
15-19	0.11792785662132396	39.77617022843153	39.80378080880593	20.302121106141207
20-24	20.023683742276706	0.08307716850430419	39.951773519612715	39.94146556960627
25-29	20.039513808358027	0.04442235598014493	59.236720844761045	20.67934299090078
30-34	0.14701100128235806	20.166522478080267	0.17376258582288734	79.51270393481448
35-39	20.020247758941228	0.13878918401531468	40.318932881746946	39.52203017529651
40-44	29.645664218528538	10.716341168602476	29.169657812873893	30.468336799995093
45-49	10.516808707763483	19.95864548628368	20.003926838097694	49.52061896785515
50-54	39.710296452825	0.4362487759264304	28.538769078981275	31.31468569226729
55-59	20.449134863173395	29.057675788440303	39.93226162719352	10.560927721192783
60-64	20.107006994723278	10.165173640937539	29.612590501902076	40.11522886243711
65-69	50.05313535403116	29.792244447171434	0.22469014603018778	19.92993005276721
70-74	49.99668671002577	9.797889311571971	0.3129218308994969	39.89250214750276
75-79	0.40802552460424596	0.18259909191311818	39.90440544852129	59.50496993496135
80-84	19.895692723033502	29.08050067492944	0.20554669284574795	50.81825990919131
85-89	49.67946987360412	10.228003436004418	29.78169100503129	10.310835685360168
90-94	0.12737759234261872	0.09314026260890908	60.03350104307277	39.7459811019757
95-99	10.315498834212788	39.87299055098785	10.180758375260767	39.630752239538594
100-104	0.09878561190630461	9.996244919597103	59.47973729162985	30.42523217686674
105-109	20.085655207112573	39.590622104688336	0.42005411740162846	39.903668570797464
110-114	0.13179612097264065	19.943305579246406	59.12933568128409	20.795562618496863
115-119	20.044545616306394	0.4838660195485308	19.774326753753552	59.69726161039153
120-124	0.17585087649329054	40.154130286724055	9.644678148718548	50.025340688064105
125-129	40.429625902723664	48.71989642837421	10.682357849784328	0.16811981911780044
130-134	0.12578307634725946	19.89102890556452	49.73524196368857	30.247946054399648
135-139	0.10308076500653458	0.5711165242147762	19.781444235146857	79.54435847563182
140-144	0.09939930911344405	10.222053147951577	40.183091073083034	49.495456469851945
145-149	30.135048073678206	30.095288350032828	10.173212499769908	29.59645107651906
150-154	0.4438614101956819	39.577662861672806	20.13034824161179	39.84812748651972
155-159	29.82265178047697	1.5381141596330319	48.249956436051804	20.389277623838193
160-164	20.14296408104161	21.343862361791164	20.636773061394788	37.87640049577243
165-169	10.607812097338291	38.955196406877	30.366675256783125	20.070316239001585
170-174	29.68560173765784	29.9710390359435	20.190332437506903	20.153026788891754
175-179	10.130692486102419	18.966608990170453	40.1446821043331	30.75801641939403
180-184	40.10234510179288	10.497981322632501	29.280148240866865	20.119525334707753
185-189	39.84059198173986	20.129956190406066	10.75494852065923	29.274503307194838
190-194	49.83494704806783	10.407907815778817	9.72204837462725	30.0350967615261
195-199	10.525101547448122	10.63750935709114	59.82697051135736	19.010418584103377
200-204	0.22015241320914478	31.078182331357606	19.925020554921524	48.776644700511724
205-209	19.8553178956669	20.712120654321442	28.953969247383082	30.478592202628572
210-214	9.634427567943458	48.44140428627717	31.22477943001975	10.699388715759621
215-219	2.058437335100443	57.441372455178	0.11461669673207427	40.38557351298948
220-224	21.12775957491195	9.730024911337727	19.985151370123575	49.15706414362675
225-229	29.910662788842668	9.818871258697492	10.87692817435482	49.39353777810502
230-234	10.396249800586583	9.333529678852361	59.69456000196346	20.5756605185976
235-239	19.98662396151628	10.954730086269313	0.17572923952926162	68.88291671268514
240-244	19.768557719446797	49.05815508841685	20.308507896771346	10.864779295365018
245-249	8.845882423599363	12.087341468053372	39.48321338124743	39.58356272709983
250-254	20.240277828909424	49.208604842371365	29.46054068647302	1.0905766422461927
255-259	20.17744726282075	19.134607124888024	20.804403048264184	39.883542564027046
260-264	19.846359631361288	11.144571659978647	10.616279497846335	58.39278921081373
265-269	9.45096884242045	10.113021389389978	20.160635177754052	60.27537459043552
270-274	10.661807115070745	29.206518671231702	59.50950435028041	0.6221698634171483
275-279	29.563376652063468	10.89128594043368	0.2628575635975408	59.282479843905314
280-284	50.25488102688706	0.21426204763833157	20.223343027893335	29.307513897581266
285-289	39.98257433518634	9.455877480396127	19.83322902477635	30.728319159641178
290-294	39.546687282946166	30.426069776288823	10.10418584103376	19.923057099731253
295-299	30.593065211759896	19.94436061979225	39.657422492207175	9.805151676240683
300-301	32.44804605042157	3.253472236622195	0.1285629564503353	64.16991875650591
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.6666666666666666
44	0.6666666666666666
45	0.3333333333333333
46	4.5
47	210.58333333333334
48	216.0
49	36.25
50	175.08333333333368
51	2790.9166666666674
52	16277.083333333341
53	5720.333333333333
54	628.5833333333334
55	1777.5833333333333
56	18664.583333333332
57	6059.999999999992
58	128.16666666666663
59	27.333333333333332
60	7.166666666666666
61	1.3333333333333333
62	0.8333333333333333
63	0.5
64	0.75
65	1.3333333333333333
66	0.5833333333333333
67	1.3333333333333333
68	2.0
69	0.75
70	1.0
71	1.0
72	1.25
73	0.5
74	1.0
75	0.25
76	1.75
77	2.5
78	1.0
79	1.5
80	2.0
81	1.25
82	0.75
83	0.5
84	0.75
85	0.5
86	0.75
87	0.75
88	1.0833333333333333
89	1.3333333333333333
90	1.0
91	0.75
92	0.75
93	1.25
94	1.25
95	1.25
96	1.25
97	0.75
98	1.25
99	9.25
100	11.000000000000002
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.227151964363507E-4
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.6483083606376321
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	1.9244315183644418
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	1.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	1.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	1.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	5.0
295-299	395.0
300-302	162578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	3.0795000644246873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.231121737397885	1.85481743270688
2	10.0418410041841	0.6184770003865481
3	4.781829049611477	0.44176928599039156
4	4.0247061167563265	0.4957633098336616
5	3.327356047021319	0.5123296580583012
6	2.510460251046025	0.4638577502899111
7	2.211595935445308	0.4767426877979642
8	1.5540944411237299	0.382866714525006
9	1.235305837816298	0.3423711966425534
>10	8.965929468021518	5.396334542063185
>50	0.3785614664275752	0.747326375467079
>100	0.3785614664275752	2.2922917395279203
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.35863717872086076	85.9750523067106
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	9498	5.827673164356582	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	9348	5.735637896441916	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	9235	5.666304661279535	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	8515	5.224535375289144	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	8320	5.10488952700008	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	8289	5.085868904964382	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	8107	4.974199446561255	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	7943	4.873574220307889	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	7880	4.83491940778373	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	7607	4.6674152201790395	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	7464	4.5796749314337255	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	7208	4.422601407526031	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	7140	4.38087875273805	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	7020	4.3072505384063176	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	6954	4.266755020523864	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	6800	4.172265478798142	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	6610	4.0556874727729	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	6185	3.794920880348016	No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	326	0.20002331560120507	No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	300	0.1840705358293298	No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	296	0.18161626201827208	No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	282	0.17302630367957	No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	282	0.17302630367957	No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	274	0.16811775605745455	No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	264	0.16198207152981023	No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	235	0.14418858639964166	No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	217	0.13314435424988189	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
