##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IAFS1_cleaned_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	162981
Sequences flagged as poor quality	0
Sequence length	51-301
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.79438094010958	40.0	40.0	40.0	40.0	40.0
2	39.327344905234355	40.0	40.0	40.0	40.0	40.0
3	39.380197691755484	40.0	40.0	40.0	40.0	40.0
4	39.259349249299	40.0	40.0	40.0	40.0	40.0
5	39.32038703898	40.0	40.0	40.0	40.0	40.0
6	39.35511501340647	40.0	40.0	40.0	40.0	40.0
7	39.417821709278996	40.0	40.0	40.0	40.0	40.0
8	39.5234045686307	40.0	40.0	40.0	40.0	40.0
9	39.066099729416315	40.0	40.0	40.0	40.0	40.0
10-14	39.61987225504813	40.0	40.0	40.0	40.0	40.0
15-19	39.51578650272118	40.0	40.0	40.0	40.0	40.0
20-24	39.49727146109056	40.0	40.0	40.0	40.0	40.0
25-29	39.659783655763555	40.0	40.0	40.0	40.0	40.0
30-34	39.55159435762451	40.0	40.0	40.0	40.0	40.0
35-39	39.667956387554376	40.0	40.0	40.0	40.0	40.0
40-44	39.58170829728619	40.0	40.0	40.0	40.0	40.0
45-49	39.52509310901271	40.0	40.0	40.0	40.0	40.0
50-54	39.607025297645656	40.0	40.0	40.0	40.0	40.0
55-59	39.51860105534422	40.0	40.0	40.0	40.0	40.0
60-64	39.507335869431834	40.0	40.0	40.0	40.0	40.0
65-69	39.33303718247638	40.0	40.0	40.0	40.0	40.0
70-74	39.49288010798871	40.0	40.0	40.0	40.0	40.0
75-79	39.50860719106639	40.0	40.0	40.0	40.0	40.0
80-84	39.551110565713586	40.0	40.0	40.0	40.0	40.0
85-89	39.64884034850902	40.0	40.0	40.0	40.0	40.0
90-94	39.53829917781323	40.0	40.0	40.0	40.0	40.0
95-99	39.43520431954841	40.0	40.0	40.0	40.0	40.0
100-104	39.35243258108765	40.0	40.0	40.0	40.0	40.0
105-109	39.4199792611318	40.0	40.0	40.0	40.0	40.0
110-114	39.438062572478664	40.0	40.0	40.0	40.0	40.0
115-119	39.348986065689445	40.0	40.0	40.0	40.0	40.0
120-124	39.26441566091337	40.0	40.0	40.0	40.0	40.0
125-129	39.40232299866854	40.0	40.0	40.0	40.0	40.0
130-134	39.411192853066964	40.0	40.0	40.0	40.0	40.0
135-139	38.99592462832635	40.0	40.0	40.0	40.0	40.0
140-144	39.49015762766983	40.0	40.0	40.0	40.0	40.0
145-149	39.09176519674314	40.0	40.0	40.0	40.0	40.0
150-154	39.26731664815713	40.0	40.0	40.0	40.0	40.0
155-159	39.363519825771114	40.0	40.0	40.0	40.0	40.0
160-164	39.32995128176809	40.0	40.0	40.0	40.0	40.0
165-169	39.3559228852974	40.0	40.0	40.0	40.0	40.0
170-174	39.186673047896036	40.0	40.0	40.0	40.0	40.0
175-179	39.43421566100947	40.0	40.0	40.0	40.0	40.0
180-184	39.372887138141344	40.0	40.0	40.0	40.0	40.0
185-189	39.31772877320866	40.0	40.0	40.0	40.0	40.0
190-194	39.32782093288665	40.0	40.0	40.0	40.0	40.0
195-199	39.284310765870245	40.0	40.0	40.0	40.0	40.0
200-204	39.50768324559143	40.0	40.0	40.0	40.0	40.0
205-209	39.31554933794746	40.0	40.0	40.0	40.0	40.0
210-214	39.4165691074869	40.0	40.0	40.0	40.0	40.0
215-219	39.39878020346305	40.0	40.0	40.0	40.0	40.0
220-224	39.39187374983127	40.0	40.0	40.0	40.0	40.0
225-229	39.426518916663596	40.0	40.0	40.0	40.0	40.0
230-234	39.25547251776314	40.0	40.0	40.0	40.0	40.0
235-239	39.31887739449496	40.0	40.0	40.0	40.0	40.0
240-244	39.35501969590988	40.0	40.0	40.0	40.0	40.0
245-249	39.255285989520054	40.0	40.0	40.0	40.0	40.0
250-254	39.35348329222349	40.0	40.0	40.0	40.0	40.0
255-259	39.0046214826541	40.0	40.0	40.0	40.0	40.0
260-264	39.184513247186736	40.0	40.0	40.0	40.0	40.0
265-269	38.84766287474383	40.0	40.0	40.0	36.8	40.0
270-274	38.22618512928125	40.0	36.8	40.0	33.6	40.0
275-279	38.9403625029145	40.0	40.0	40.0	40.0	40.0
280-284	38.57023647363448	40.0	40.0	40.0	36.8	40.0
285-289	39.077877995803114	40.0	40.0	40.0	40.0	40.0
290-294	39.007031547760356	40.0	40.0	40.0	40.0	40.0
295-299	39.04275424003693	40.0	40.0	40.0	40.0	40.0
300-301	38.70266545756611	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12770731547242775
1101	2	0.17956929949101408
1101	3	0.0256885781904046
1101	4	-0.10133823563110411
1101	5	0.06129532091330958
1101	6	0.12238190150682726
1101	7	0.1605384939366843
1101	8	0.18055406747824065
1101	9	0.3135051267796456
1101	10-14	0.06500247004744608
1101	15-19	0.10764237854942138
1101	20-24	-0.11050929242416885
1101	25-29	0.10743430651113783
1101	30-34	-0.015462955609358175
1101	35-39	0.07121101539242147
1101	40-44	0.13493622898406699
1101	45-49	0.07568436770004183
1101	50-54	0.054009942711481074
1101	55-59	0.0023222191018987814
1101	60-64	-0.04814003362490382
1101	65-69	0.0988559507772564
1101	70-74	-0.010952389135489682
1101	75-79	0.03731007435458622
1101	80-84	0.10507588677297974
1101	85-89	0.08638923857612468
1101	90-94	0.028650183099863114
1101	95-99	0.03699807695014812
1101	100-104	-0.0640917772963121
1101	105-109	0.13641026347746532
1101	110-114	0.08246715903714374
1101	115-119	0.13542619450717552
1101	120-124	0.09317014200035345
1101	125-129	0.17583039969177605
1101	130-134	0.13154739275342564
1101	135-139	0.18713262121388397
1101	140-144	0.13676916371608883
1101	145-149	0.15045810735026066
1101	150-154	0.10536700877673866
1101	155-159	0.11086241796431295
1101	160-164	0.055742741498349346
1101	165-169	0.1294469636882738
1101	170-174	0.2041655468495307
1101	175-179	0.1719411194878333
1101	180-184	0.06614772656205048
1101	185-189	0.12470674984943031
1101	190-194	0.1390862403751001
1101	195-199	0.14431090416575643
1101	200-204	0.0831035659840964
1101	205-209	0.13917814786198335
1101	210-214	0.041727952265468105
1101	215-219	0.12785325918143542
1101	220-224	0.18011703610167018
1101	225-229	0.26108347850878744
1101	230-234	0.12153394371614468
1101	235-239	0.010287983868828121
1101	240-244	0.08307450260875981
1101	245-249	0.03268819012652813
1101	250-254	0.14604558669851997
1101	255-259	0.17390618430643912
1101	260-264	0.14835312014646007
1101	265-269	0.07045932296536961
1101	270-274	0.22655382500278876
1101	275-279	0.1519121476833405
1101	280-284	0.22274764365473487
1101	285-289	0.10034209297258201
1101	290-294	-0.033235411441260965
1101	295-299	0.011321753527177236
1101	300-301	0.030180390188895956
1102	1	0.06728005625036815
1102	2	-0.040462922800955425
1102	3	0.05991953251426452
1102	4	0.19334927090751108
1102	5	0.11757971402526834
1102	6	0.22427966896231766
1102	7	0.06085862449973689
1102	8	0.06656247875186239
1102	9	-0.13949008172369304
1102	10-14	0.07515458437380573
1102	15-19	0.0334892598918799
1102	20-24	0.08972086680417135
1102	25-29	0.10163429395699808
1102	30-34	0.13013995695106217
1102	35-39	0.06223899020044854
1102	40-44	0.1902497686237581
1102	45-49	-0.04645067299629346
1102	50-54	0.0625467577262313
1102	55-59	-0.028845525128424754
1102	60-64	-0.006242684640739071
1102	65-69	0.1571908533780615
1102	70-74	0.059526551295114416
1102	75-79	0.15462432539553816
1102	80-84	0.10905136435338392
1102	85-89	0.05946479357372425
1102	90-94	0.03223229763546698
1102	95-99	0.03549994960932423
1102	100-104	0.03383888200524865
1102	105-109	0.1144739966601449
1102	110-114	0.0033677093269446345
1102	115-119	0.1026933675241466
1102	120-124	0.18104075066689518
1102	125-129	0.055226964042205395
1102	130-134	0.0675882355546733
1102	135-139	0.12570103081362305
1102	140-144	0.1032328717904889
1102	145-149	0.0639098680683503
1102	150-154	-0.02677786599571874
1102	155-159	-0.07785977984708126
1102	160-164	-0.011932441059769872
1102	165-169	-0.08016532262980292
1102	170-174	0.10649433976899303
1102	175-179	0.1054209177846559
1102	180-184	0.007704021416948592
1102	185-189	3.2033342861126357E-4
1102	190-194	0.007401684059317404
1102	195-199	0.12179714692096866
1102	200-204	0.03449393695892411
1102	205-209	0.011921018156790808
1102	210-214	0.16613110753925753
1102	215-219	0.06834663687272524
1102	220-224	0.05347924395637449
1102	225-229	0.05857683527651858
1102	230-234	0.0328263924010912
1102	235-239	-0.018854359152115308
1102	240-244	0.05892033777290351
1102	245-249	-0.026667782505448656
1102	250-254	-0.019945248779478675
1102	255-259	-0.11976025220099729
1102	260-264	-0.020064849012200625
1102	265-269	0.012099312085112501
1102	270-274	0.011292175807305682
1102	275-279	-0.0072576052093396015
1102	280-284	-0.20483798603998338
1102	285-289	-0.08386061453688853
1102	290-294	0.02259703264050472
1102	295-299	0.05858390488826615
1102	300-301	-0.021605436187350335
1103	1	0.09518802842800511
1103	2	-0.16707608491973502
1103	3	0.3935310894966051
1103	4	0.1909522671621957
1103	5	0.2521971565078829
1103	6	0.30076977062095267
1103	7	0.18165049180845472
1103	8	0.234866384583853
1103	9	0.11617604989749708
1103	10-14	-0.017121499093292414
1103	15-19	0.13051654721130035
1103	20-24	0.09461760302675515
1103	25-29	0.12650320781468594
1103	30-34	0.23255194196978124
1103	35-39	0.043850547182792354
1103	40-44	0.05522141121337398
1103	45-49	0.03438827831456592
1103	50-54	0.12683782246672592
1103	55-59	0.024847358766706407
1103	60-64	0.020227085289704405
1103	65-69	-0.024947188355483263
1103	70-74	0.03655811897836969
1103	75-79	0.08032577001832664
1103	80-84	0.01458373460485518
1103	85-89	-2.976462336619079E-4
1103	90-94	0.04170046242947478
1103	95-99	-0.02700192304985194
1103	100-104	0.11644134321445421
1103	105-109	0.08678004267405726
1103	110-114	0.13702635031142307
1103	115-119	0.01596463558193051
1103	120-124	0.0011541803084185176
1103	125-129	-0.15332948325580986
1103	130-134	-0.1210559592018825
1103	135-139	-0.1405204780146363
1103	140-144	-0.016437540194530698
1103	145-149	-0.029479109994788644
1103	150-154	-0.15798813886891594
1103	155-159	-0.0703006573216669
1103	160-164	-0.08245571553809583
1103	165-169	-0.16413750026489993
1103	170-174	-0.19778816424383905
1103	175-179	-0.07279631067977022
1103	180-184	-0.07258704332355137
1103	185-189	-0.128415889150304
1103	190-194	-0.16201511636871402
1103	195-199	-0.09357363959588127
1103	200-204	-0.08419199134498001
1103	205-209	-0.11800514556711761
1103	210-214	-0.04641463884919261
1103	215-219	-0.08987273177361743
1103	220-224	-0.15987977155994315
1103	225-229	0.04212311671044233
1103	230-234	-0.23023620791993693
1103	235-239	-0.11437495840304734
1103	240-244	-0.04415082327579256
1103	245-249	-0.11890106233423126
1103	250-254	-0.06606188869387353
1103	255-259	-0.10965859356001317
1103	260-264	-0.20315313151621694
1103	265-269	-0.08469683558744379
1103	270-274	-0.06258690391447175
1103	275-279	-0.13910993265717764
1103	280-284	0.02060877693486418
1103	285-289	-0.03043311319927966
1103	290-294	-0.13979141037716403
1103	295-299	-0.059713048655893886
1103	300-301	-0.03838519093688575
1104	1	0.10515249182530084
1104	2	0.17863820079455195
1104	3	-0.1246352218040343
1104	4	0.02388759054527867
1104	5	-0.07424207590150189
1104	6	-0.13233612845509413
1104	7	0.025445800976903854
1104	8	-0.0737192586286497
1104	9	-0.09066119246213589
1104	10-14	-0.06993457656307811
1104	15-19	-0.028923395930128493
1104	20-24	-0.13555723705853495
1104	25-29	-0.06016345885198149
1104	30-34	0.004532041472273818
1104	35-39	-0.06510183376958878
1104	40-44	-0.06560019504106407
1104	45-49	-0.03552216944662945
1104	50-54	-0.08395109011323143
1104	55-59	-0.0746949297905104
1104	60-64	-0.1685547199697126
1104	65-69	0.05305423310861812
1104	70-74	-0.07610428329597596
1104	75-79	-0.03666641192907605
1104	80-84	-0.03290738124447756
1104	85-89	-0.08275109328413066
1104	90-94	-0.11543386592873617
1104	95-99	-0.08787754494039746
1104	100-104	-0.1534605757620895
1104	105-109	-0.100227775378535
1104	110-114	-0.06618900150093765
1104	115-119	-0.05653856271231206
1104	120-124	-0.15426586233551376
1104	125-129	-0.08014113926149946
1104	130-134	-0.24541416815710448
1104	135-139	-0.07257165072252292
1104	140-144	-0.059442515318913536
1104	145-149	-0.1688309223615292
1104	150-154	-0.136029361328049
1104	155-159	-0.1632530382740427
1104	160-164	-0.14981605668948106
1104	165-169	-0.09687950452929073
1104	170-174	-0.2237924286361661
1104	175-179	-0.27502516640115715
1104	180-184	-0.18858420039533996
1104	185-189	-0.14495888843956806
1104	190-194	-0.14394830755563248
1104	195-199	-0.2656532415859303
1104	200-204	-0.08030286554540567
1104	205-209	-0.1662666949629994
1104	210-214	-0.12790433323440453
1104	215-219	-0.07300279573949098
1104	220-224	-0.1251221311903592
1104	225-229	-0.1514404938083942
1104	230-234	-0.2467308774295276
1104	235-239	-0.17152918725876987
1104	240-244	-0.1433548033752885
1104	245-249	-0.13130191521269552
1104	250-254	-0.0787299768246541
1104	255-259	-0.10073179896157569
1104	260-264	-0.1513578223477765
1104	265-269	-0.07777714830955063
1104	270-274	-0.2732905286479479
1104	275-279	-0.2019414891603759
1104	280-284	-0.23019435028616897
1104	285-289	-0.09366552258093463
1104	290-294	-0.17688309481211917
1104	295-299	-0.11427651212778756
1104	300-301	-0.19918663133520909
1201	1	0.09518802842800511
1201	2	0.16816201031836187
1201	3	0.10400727997279802
1201	4	0.251904648114575
1201	5	0.11505429936502765
1201	6	0.2321983420495286
1201	7	0.1054600156179788
1201	8	0.2043901941076669
1201	9	0.04760462132606591
1201	10-14	0.10935469138291154
1201	15-19	-0.015769167074410007
1201	20-24	0.07328426969340995
1201	25-29	-0.009115839804366033
1201	30-34	0.022266227684070827
1201	35-39	-0.03691135757910757
1201	40-44	0.11007855407051892
1201	45-49	0.06791208783837277
1201	50-54	-0.01030503467612931
1201	55-59	0.006561644480996165
1201	60-64	0.0034651805278045344
1201	65-69	0.004005192596892471
1201	70-74	0.09141526183551463
1201	75-79	-0.0034837537912011385
1201	80-84	0.02067897270008956
1201	85-89	0.011892829956813955
1201	90-94	0.0919861767151886
1201	95-99	-0.06204954209746205
1201	100-104	-0.17917770440458725
1201	105-109	0.007541947435960594
1201	110-114	-0.051926030640956355
1201	115-119	-0.06327345965616615
1201	120-124	-0.03694105778682655
1201	125-129	-0.019234245160582475
1201	130-134	0.13951546936953463
1201	135-139	-0.009472858967022546
1201	140-144	0.027752935995948746
1201	145-149	0.019282794767121914
1201	150-154	0.07972614684538115
1201	155-159	0.0683660093449987
1201	160-164	-0.00474142982380954
1201	165-169	-0.019375595502999943
1201	170-174	0.07802135956568179
1201	175-179	0.014060832177378302
1201	180-184	-0.058872757609265136
1201	185-189	-0.040034936769352214
1201	190-194	0.05284202648842751
1201	195-199	-0.011287925310170976
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2404	210-214	0.0496723502771772
2404	215-219	-0.10306508272974213
2404	220-224	-0.05263417725922892
2404	225-229	0.07629484524436947
2404	230-234	-0.014506174174151454
2404	235-239	0.053268086216370136
2404	240-244	0.022410856514241573
2404	245-249	-0.06231613545009651
2404	250-254	0.00842480046998162
2404	255-259	-0.06265446907557504
2404	260-264	0.05483561988985741
2404	265-269	-0.022823569745661132
2404	270-274	0.18564706683918786
2404	275-279	-0.0754518899125145
2404	280-284	0.0862705692108321
2404	285-289	-0.03441061601142792
2404	290-294	0.06428508006153777
2404	295-299	0.10837009734454028
2404	300-301	0.15846591730852566
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	9.0
21	33.0
22	57.0
23	96.0
24	231.0
25	312.0
26	300.0
27	277.0
28	325.0
29	442.0
30	467.0
31	618.0
32	664.0
33	832.0
34	1105.0
35	1396.0
36	2050.0
37	3056.0
38	6386.0
39	126130.0
40	18195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.07178750897343862	0.006135684527644326	0.09448954172572263	99.8275872647732
2	0.008590116457435973	0.014725913927033095	0.16075789370344462	99.81592607591209
3	0.009817095244230923	0.1355986280609396	99.6238825384554	0.23070173823942666
4	0.07669605659555408	99.47969395205575	0.10860161613930458	0.33500837520938026
5	0.20002331560120507	0.14234788104134838	0.08037746731214067	99.57725133604531
6	99.77727465164651	0.06074327682367883	0.01411207441358195	0.14786999711622828
7	99.91287327970745	0.05399402384327007	0.02024775894122628	0.012884937508053087
8	99.7533454819887	0.10246593161166026	0.09080813100913604	0.05338045539050564
9	28.826673047778574	21.670010614734235	26.3110423914444	23.19227394604279
10-14	59.83556365465913	10.254324123670857	9.767028058485346	20.143084163184668
15-19	39.74475552364999	20.332431387707768	19.933857320791994	19.98895576785024
20-24	59.928212491026564	39.83654536418356	0.20272301679336857	0.032519127996514935
25-29	19.516753486602735	19.701928445647038	59.68573023849406	1.095587829256171
30-34	0.4252029377657518	59.61651971702223	0.22174363882906598	39.73653370638296
35-39	59.86219252550911	0.5389585289082777	19.930912192218724	19.667936753363886
40-44	39.65787423073856	49.618176351844696	0.06773795718519336	10.656211460231562
45-49	20.09743467029899	19.926617213049376	20.536258827716114	39.43968928893552
50-54	40.288280063124155	20.06597112290803	19.743232928622092	19.90251588534572
55-59	20.065284083936678	29.732237084304824	10.241992882562277	39.960485949196226
60-64	39.987728555650996	0.06270708062338938	0.33820100625843663	59.61136335746717
65-69	30.07669652718125	20.0589029328752	40.633206528408394	9.231194011535157
70-74	39.98834212786845	51.209964412811395	8.685114738004664	0.11657872131549885
75-79	40.3448275862069	19.817032764756412	1.0119033010185299	38.82623634801816
80-84	40.067738372806474	0.10013498588783899	39.74524481531476	20.08688182599092
85-89	39.254141612467784	0.5238679592588048	50.23499815928335	9.98699226899006
90-94	20.06908823168487	59.667321143698615	10.368143330469996	9.895447294146521
95-99	9.858755675543012	10.065406798380168	59.68204687691742	20.393790649159406
100-104	21.371193379277848	20.20859103492961	10.353468418055801	48.06674716773674
105-109	60.749912565422534	38.72204394431185	0.3893753182925408	0.1386681719730763
110-114	40.99767454702753	49.46281422759988	9.356911013075305	0.18260021229728984
115-119	39.61344713122549	31.12008295547279	19.75309702477006	9.513372888531652
120-124	20.95533780425699	30.152719061965037	39.3710846182637	9.520858515514268
125-129	27.987286705649193	40.52448474956896	20.36986360205916	11.118364942722682
130-134	26.630056633063155	31.432270415206865	30.865203492474492	11.072469459255487
135-139	9.566017707802846	11.047312843986035	21.920370109032454	57.466299339178676
140-144	39.787825425361554	19.90010983010081	20.02577019125163	20.286294553286005
145-149	0.6411869013799324	29.782241883923692	30.41029825928494	39.166272955411436
150-154	39.04343504377864	10.858331441474055	10.555838482258451	39.54239503248885
155-159	29.538443036763063	60.55425613638092	0.047858985340424647	9.859441841515583
160-164	29.589760581182734	10.655916749499934	0.4272969357827437	59.32702573353459
165-169	30.53663684669096	29.54079691737535	29.180012026163038	10.742554209770644
170-174	9.798254979199648	29.445201192799026	20.110812502300924	40.6457313257004
175-179	67.48395488961701	0.4646025843978942	10.858029918148462	21.19341260783664
180-184	30.912024935880915	31.181631876695015	8.612941624022874	29.293401563401193
185-189	30.749303586987203	9.415872080894355	28.025377658334254	31.80944667378419
190-194	29.338929180625605	30.528537594031096	21.524132091447925	18.608401133895374
195-199	20.918897029046864	39.29990550871897	19.91103093669084	19.870166525543326
200-204	70.36004859551596	0.2075126704217747	19.8446416080698	9.587797125992466
205-209	20.36201205070623	48.90819619825989	9.559081593834751	21.17071015719913
210-214	47.83590423247309	20.755930248254366	30.827964510547435	0.580201008725104
215-219	40.08761918786585	20.08418314128287	19.37439408999988	20.453803580851403
220-224	28.43095387107462	20.67714660874474	39.70965406373866	11.182245456441974
225-229	32.49628784253089	1.0358453288173863	46.005104983494704	20.462761845157015
230-234	20.144927536231886	50.27574273828369	20.162844064843107	9.416485660641314
235-239	49.36273607480764	28.896415467118263	1.0194013915988662	20.72144706647523
240-244	30.893985691320303	38.55489697996048	9.96846200100627	20.582655327712942
245-249	49.44274687381119	9.771134754384027	20.378455987924752	20.40766238388003
250-254	29.09484715728503	1.2083839536624574	50.52878302592988	19.16798586312263
255-259	10.778755414841266	39.844518892120405	11.014983617420757	38.36174207561756
260-264	39.97447508252648	30.01386690228129	29.52054878572568	0.4911092294665537
265-269	20.39674066438415	50.13290137319147	11.557142681834359	17.91321528059002
270-274	10.189227993962376	10.166402827375475	37.64581722686498	41.998551951797175
275-279	39.708426904244746	19.741805642479353	19.872743560480554	20.677023892795347
280-284	18.13569929683761	47.89456245628244	2.2171090576642247	31.75262918921572
285-289	41.0370724883113	20.62732393329161	28.82536293242033	9.510240645976758
290-294	29.275015155494145	20.404817360956013	20.42248854440989	29.897678939139954
295-299	38.62053556631233	29.508075970718544	21.0293195118448	10.842068951124325
300-301	46.13586528037843	29.025204493939093	24.83246279688578	0.006467428796688677
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.6666666666666666
46	0.6666666666666666
47	0.5833333333333333
48	1.5
49	13.583333333333334
50	818.5000000000001
51	592.8333333333333
52	72.91666666666667
53	337.7499999999998
54	13739.66666666668
55	34069.08333333328
56	4063.6666666666742
57	146.74999999999997
58	28.083333333333332
59	7.583333333333333
60	3.25
61	1.5
62	0.75
63	0.0
64	0.25
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.25
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001840705358293298
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	1.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	1.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	1.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	6.0
295-299	380.0
300-302	162592.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	2.0345929893668586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	50.15078407720145	1.0203643369472515
2	11.158021712907116	0.4540406550456802
3	6.242460796139928	0.3810260091667127
4	5.036188178528348	0.40986372644664104
5	3.1363088057901085	0.319055595437505
6	2.9553679131483714	0.3607782502254864
7	2.2316043425814236	0.31782845853197617
8	1.9300361881785282	0.31414704781538955
9	1.9300361881785282	0.35341542879231325
>10	13.811821471652594	4.8698928095913026
>50	0.27141133896260555	0.327645553776207
>100	0.6634499396863691	2.778237954117351
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.39203860072376356	68.37054625999349
>10k+	0.09047044632086852	19.723157914112686
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	11148	6.840061111417896	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	10596	6.501371325491928	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	10401	6.381725477202864	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	9888	6.06696486093471	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9799	6.012357268638676	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9484	5.819083206017879	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	9341	5.731342917272565	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	9287	5.698210220823286	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9229	5.662623250562949	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	8588	5.269325872340948	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8566	5.25582736638013	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	8529	5.2331253336278465	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	8326	5.108570937716666	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	7547	4.6306011130131735	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	6713	4.118885023407636	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	6134	3.76362888925703	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	383	0.23499671740877773	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	336	0.20615900012884938	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	332	0.20370472631779163	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	322	0.1975690417901473	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	321	0.1969554733373829	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	301	0.18468410428209422	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	270	0.16566348224639682	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	219	0.13437149115541075	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	210	0.12884937508053088	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	194	0.11903227983629994	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	187	0.11473730066694891	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	168	0.10307950006442469	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	166	0.10185236315889581	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	166	0.10185236315889581	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
