FastQCFastQC Report
Fri 1 Nov 2024
DPFS2_cleaned_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDPFS2_cleaned_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131642
Sequences flagged as poor quality0
Sequence length15-301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG110218.371948162440559No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG107108.135701371902584No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG106988.126585740113338No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG96747.3487184940976285No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG93497.101836799805533No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG92076.993968490299449No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG91476.948390331353216No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG88986.759240971726349No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG81136.162926725513134No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG13681.0391820239741116No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG13431.0201911244131812No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG13100.9951231369927531No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG12020.9130824508895337No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG11630.8834566475744823No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG11310.8591482961364914No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG11050.8393977605931238No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG10570.8029352334361374No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG10220.7763479740508349No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3690.2803056775193327No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3310.2514395101867185No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3140.23852569848528588No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3110.2362467905379742No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2960.22485225080141596No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2920.2218137068716671No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2880.21877516294191826No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA2830.21497698302973214No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA2570.19522644748636453No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2550.1937071755214901No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA2430.1845915437322435No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA2370.1800337278376202No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2330.17699518390787136No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA2250.17091809604837363No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA2160.16408137220643867No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA2120.1610428282766898No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA2100.1595235563118154No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA1880.1428115646981966No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1850.14053265675088497No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1720.13065738897920118No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1590.12078212120751736No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1550.11774357727776849No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1520.11546466933045685No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1480.11242612540070798No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1460.11090685343583355No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1360.1033104936114614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)