FastQCFastQC Report
Fri 1 Nov 2024
DPFS1_cleaned_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDPFS1_cleaned_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141413
Sequences flagged as poor quality0
Sequence length15-301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG121148.566397714495839No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG121118.564276268801313No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG117158.284245437123886No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG102537.250394235324899No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG101947.208672469999222No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG99167.012085168973149No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG96956.85580533614307No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG94996.71720421743404No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG91896.497988162333026No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG18371.2990319136147312No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG18191.2863032394475755No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG17811.2594315939835798No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG15311.082644452773083No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG15191.0741586699949792No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG14931.0557728073090875No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG14361.0154653391130943No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG14120.9984937735568865No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG13390.9468719283234215No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4060.287102317325847No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3820.2701307517696393No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3700.26164496899153544No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3360.23760191778690784No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3340.23618762065722387No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3240.229116135008804No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3130.22133750079554215No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2920.2064873809338604No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2370.16759420986755108No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1940.1371868215793456No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1800.1272867416715578No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1670.11809381032861194No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1500.10607228472629814No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1500.10607228472629814No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1460.1032436904669302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)