##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	DCMS2_cleaned_R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93887
Sequences flagged as poor quality	0
Sequence length	15-301
%GC	52
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.945562218411496	34.0	34.0	34.0	20.0	34.0
2	32.55169512286046	34.0	34.0	34.0	34.0	34.0
3	32.746099033945065	34.0	34.0	34.0	34.0	34.0
4	33.309606228764366	34.0	34.0	34.0	34.0	34.0
5	32.67713314942431	34.0	34.0	34.0	34.0	34.0
6	32.90864549937691	34.0	34.0	34.0	34.0	34.0
7	32.48217538104317	34.0	34.0	34.0	34.0	34.0
8	32.56690489631152	34.0	34.0	34.0	34.0	34.0
9	33.0136334103763	34.0	34.0	34.0	34.0	34.0
10-14	33.06199154302513	34.0	34.0	34.0	34.0	34.0
15-19	33.025478947406704	34.0	34.0	34.0	34.0	34.0
20-24	32.84827042021187	34.0	34.0	34.0	34.0	34.0
25-29	32.94846905493899	34.0	34.0	34.0	34.0	34.0
30-34	32.60352272610694	34.0	34.0	34.0	31.2	34.0
35-39	32.577458443398285	34.0	34.0	34.0	31.2	34.0
40-44	32.82733505636386	34.0	34.0	34.0	34.0	34.0
45-49	32.79764365250703	34.0	34.0	34.0	34.0	34.0
50-54	32.71425139228977	34.0	34.0	34.0	34.0	34.0
55-59	32.60211891242973	34.0	34.0	34.0	34.0	34.0
60-64	32.554815036799866	34.0	34.0	34.0	34.0	34.0
65-69	32.120689679181844	34.0	34.0	34.0	25.6	34.0
70-74	31.958502185689973	34.0	34.0	34.0	25.6	34.0
75-79	32.58955285768667	34.0	34.0	34.0	34.0	34.0
80-84	32.44372237807739	34.0	34.0	34.0	31.2	34.0
85-89	32.4601897000997	34.0	34.0	34.0	31.2	34.0
90-94	32.394065045068274	34.0	34.0	34.0	28.4	34.0
95-99	31.82699326894168	34.0	34.0	34.0	22.8	34.0
100-104	32.23561502829397	34.0	34.0	34.0	25.6	34.0
105-109	31.87924116189679	34.0	34.0	34.0	20.0	34.0
110-114	32.25225477899309	34.0	34.0	34.0	25.6	34.0
115-119	32.06704758256767	34.0	34.0	34.0	20.0	34.0
120-124	31.948123072352484	34.0	34.0	34.0	20.0	34.0
125-129	31.88718128002635	34.0	34.0	34.0	20.0	34.0
130-134	31.748247155415157	34.0	34.0	34.0	25.6	34.0
135-139	31.734995116063452	34.0	34.0	34.0	20.0	34.0
140-144	31.84745959824147	34.0	34.0	34.0	20.0	34.0
145-149	31.302425177892598	34.0	34.0	34.0	20.0	34.0
150-154	31.449472870315542	34.0	34.0	34.0	20.0	34.0
155-159	31.333754656990227	34.0	34.0	34.0	20.0	34.0
160-164	31.180544538187768	34.0	34.0	34.0	20.0	34.0
165-169	31.493187928327426	34.0	34.0	34.0	20.0	34.0
170-174	31.02777743264728	34.0	34.0	34.0	20.0	34.0
175-179	31.20927113243232	34.0	34.0	34.0	20.0	34.0
180-184	31.3869405792886	34.0	34.0	34.0	20.0	34.0
185-189	30.90655155417168	34.0	34.0	34.0	20.0	34.0
190-194	30.564227423323292	34.0	31.2	34.0	17.8	34.0
195-199	31.15464335714959	34.0	34.0	34.0	20.0	34.0
200-204	30.165894323790788	34.0	31.2	34.0	17.8	34.0
205-209	30.155311773862074	34.0	34.0	34.0	20.0	34.0
210-214	29.72213199376828	34.0	31.2	34.0	17.8	34.0
215-219	29.58608415658498	34.0	25.6	34.0	20.0	34.0
220-224	29.956230438140892	34.0	28.4	34.0	20.0	34.0
225-229	29.143014178749365	34.0	22.8	34.0	15.6	34.0
230-234	29.84525363188725	34.0	25.6	34.0	20.0	34.0
235-239	29.01177221875299	34.0	20.0	34.0	15.6	34.0
240-244	27.511095103647072	34.0	20.0	34.0	15.6	34.0
245-249	27.922763418512563	34.0	20.0	34.0	15.6	34.0
250-254	28.114526631251657	34.0	20.0	34.0	20.0	34.0
255-259	27.311759371590774	34.0	20.0	34.0	15.6	34.0
260-264	27.832050129569843	34.0	20.0	34.0	15.6	34.0
265-269	27.145351170150043	34.0	20.0	34.0	13.4	34.0
270-274	27.06868045376774	34.0	20.0	34.0	13.4	34.0
275-279	25.601554840249076	31.2	20.0	34.0	9.0	34.0
280-284	26.160868492252398	34.0	20.0	34.0	9.0	34.0
285-289	26.242559405012845	31.2	20.0	34.0	13.4	34.0
290-294	25.044531137987427	25.6	20.0	34.0	9.0	34.0
295-299	25.06843891906922	22.8	20.0	34.0	9.0	34.0
300-301	21.975186428749932	20.0	14.5	34.0	9.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.34500970152376453
1101	2	-0.6902449216819235
1101	3	-0.8204815057025812
1101	4	-0.10765735197198723
1101	5	-0.6596292471425329
1101	6	-2.136363627514239
1101	7	-1.1815172315888027
1101	8	-0.34614701962796346
1101	9	-0.6232744763442852
1101	10-14	-0.4668168538603794
1101	15-19	-0.40672105950347515
1101	20-24	-0.6301648552912127
1101	25-29	-0.41513867781096536
1101	30-34	-0.7123102458519526
1101	35-39	-0.5585090692687587
1101	40-44	-0.6365778763114633
1101	45-49	-0.6214033186492642
1101	50-54	-0.5026147405297792
1101	55-59	-0.7003437575906943
1101	60-64	-0.6357823762658548
1101	65-69	-0.8516330158154126
1101	70-74	-0.7564664164026134
1101	75-79	-0.7058225227656933
1101	80-84	-0.5092263018306511
1101	85-89	-0.6817440656988794
1101	90-94	-0.5043573554583531
1101	95-99	-0.973256368345023
1101	100-104	-0.8861806398708936
1101	105-109	-0.935017111695057
1101	110-114	-1.140262250635459
1101	115-119	-1.0781952102598815
1101	120-124	-1.173097664322615
1101	125-129	-0.9169193009553069
1101	130-134	-1.0220977512956786
1101	135-139	-1.1106212941215077
1101	140-144	-1.1795953926076805
1101	145-149	-1.6186575500410285
1101	150-154	-1.060592113188882
1101	155-159	-1.4783969353408288
1101	160-164	-1.301247592871217
1101	165-169	-1.073965077628948
1101	170-174	-1.519828919164489
1101	175-179	-1.4289022617971021
1101	180-184	-1.584568649737701
1101	185-189	-1.574221363123165
1101	190-194	-1.669425725704908
1101	195-199	-1.700695618469279
1101	200-204	-1.9090314822225736
1101	205-209	-2.3472077231448267
1101	210-214	-1.298649704750055
1101	215-219	-1.8336382959271695
1101	220-224	-2.248824147763859
1101	225-229	-2.705287640768315
1101	230-234	-2.349219329892211
1101	235-239	-2.867395598186029
1101	240-244	-2.8421605306215945
1101	245-249	-2.893356369250938
1101	250-254	-2.8405685730612404
1101	255-259	-3.0981367042597867
1101	260-264	-3.080004124998325
1101	265-269	-3.2320080110064495
1101	270-274	-3.309166916159615
1101	275-279	-3.4132249915363246
1101	280-284	-3.618526480364551
1101	285-289	-3.7813852239671597
1101	290-294	-4.201492615617337
1101	295-299	-3.960504340084377
1101	300-301	-4.00976844596908
1102	1	0.004733888219824678
1102	2	0.12680636036935766
1102	3	-0.0020199672410399216
1102	4	-0.04086248017711824
1102	5	0.161268188754903
1102	6	-0.37277388392449495
1102	7	-0.4806197956913678
1102	8	-0.4090957375766848
1102	9	-0.04660780967761724
1102	10-14	-0.27925275129628346
1102	15-19	-0.3262338800163036
1102	20-24	-0.3751135732399362
1102	25-29	-0.27295919063148233
1102	30-34	-0.39854101508271356
1102	35-39	-0.3068936846533745
1102	40-44	-0.31742403015761766
1102	45-49	-0.2377879340338822
1102	50-54	-0.047307048222087644
1102	55-59	-0.2677540140009498
1102	60-64	-0.5635259660094469
1102	65-69	-0.6565541934032595
1102	70-74	-0.8542821808850434
1102	75-79	-0.6925271761370162
1102	80-84	-0.5576593502637017
1102	85-89	-0.5905047494595586
1102	90-94	-0.7189558231650786
1102	95-99	-0.8322554604823331
1102	100-104	-0.803821725751849
1102	105-109	-0.9398790869471654
1102	110-114	-1.0064693878307054
1102	115-119	-0.7834234560537645
1102	120-124	-1.0816586404226385
1102	125-129	-1.0325678567051781
1102	130-134	-1.002071218099033
1102	135-139	-1.2024603260618498
1102	140-144	-0.9971977564742964
1102	145-149	-1.270152052443084
1102	150-154	-1.3993325903844074
1102	155-159	-0.9650137517592441
1102	160-164	-1.011249503396634
1102	165-169	-1.2749994665228783
1102	170-174	-1.7047456311990956
1102	175-179	-1.5411000430917987
1102	180-184	-1.5841208217036709
1102	185-189	-1.626478247422991
1102	190-194	-1.5565122241121614
1102	195-199	-1.6523489677983605
1102	200-204	-1.7698349003434082
1102	205-209	-1.9820178497271037
1102	210-214	-1.3083324270910097
1102	215-219	-1.9974565986449946
1102	220-224	-1.9407755950094234
1102	225-229	-1.750939475272606
1102	230-234	-1.9460239096476037
1102	235-239	-2.41133522868304
1102	240-244	-2.3558376694229572
1102	245-249	-3.047603409408957
1102	250-254	-3.426189896651163
1102	255-259	-3.2727502047103485
1102	260-264	-3.512237740443876
1102	265-269	-3.768165873539502
1102	270-274	-3.4786336262714883
1102	275-279	-4.000470182892123
1102	280-284	-3.893678395310097
1102	285-289	-4.433994528669466
1102	290-294	-4.607588634064339
1102	295-299	-4.554926733056391
1102	300-301	-2.1046603065067835
1103	1	-0.8755804211267417
1103	2	-0.5662250705652987
1103	3	-0.06852120793583083
1103	4	-0.3874092130141733
1103	5	-0.9882148550829797
1103	6	-0.20147529004525921
1103	7	-0.342013509504433
1103	8	-0.33041500970240634
1103	9	-0.5565747244832409
1103	10-14	-0.2159483675080267
1103	15-19	-0.4935002158310198
1103	20-24	-0.34241299424903104
1103	25-29	-0.26685083661989495
1103	30-34	-0.3428710398966146
1103	35-39	-0.43618648862358356
1103	40-44	-0.33334214430153963
1103	45-49	-0.37879289681303874
1103	50-54	-0.2741184626141404
1103	55-59	-0.47718743004727315
1103	60-64	-0.4410032199383167
1103	65-69	-0.6708221834776964
1103	70-74	-0.7631490129776886
1103	75-79	-0.2359101372619179
1103	80-84	-0.5442681178402076
1103	85-89	-0.4818157502866889
1103	90-94	-0.5883677098988187
1103	95-99	-0.9282102369578027
1103	100-104	-0.4268857232624832
1103	105-109	-0.6038496294220117
1103	110-114	-0.3061204734551062
1103	115-119	-0.8619139039084445
1103	120-124	-0.6832078626796552
1103	125-129	-0.7573721160224309
1103	130-134	-0.731065893952465
1103	135-139	-1.0016731875296436
1103	140-144	-0.9173794038278764
1103	145-149	-1.0191655194020584
1103	150-154	-1.6342840466950825
1103	155-159	-1.252720685517854
1103	160-164	-1.0180572155710337
1103	165-169	-1.2414120826113475
1103	170-174	-1.3399867932215983
1103	175-179	-1.6594615565703883
1103	180-184	-1.3843330277982275
1103	185-189	-1.6299694899209882
1103	190-194	-1.5036067861506375
1103	195-199	-1.2011015310246087
1103	200-204	-2.188913847711831
1103	205-209	-1.7557020602534195
1103	210-214	-1.240338578833999
1103	215-219	-2.2233172136857036
1103	220-224	-1.7829281577889198
1103	225-229	-2.0678220088667807
1103	230-234	-2.3082638057683624
1103	235-239	-2.6076034013693814
1103	240-244	-2.7197685040435218
1103	245-249	-3.206475601439937
1103	250-254	-3.063928545599115
1103	255-259	-3.087226395186537
1103	260-264	-3.0622036585837122
1103	265-269	-3.445823659452323
1103	270-274	-3.4584787441166043
1103	275-279	-3.6895150169926474
1103	280-284	-3.881536574252653
1103	285-289	-3.834434200389733
1103	290-294	-4.126611212998355
1103	295-299	-4.07016250239057
1103	300-301	-2.8361174470048915
1104	1	-1.3508959891491834
1104	2	-0.80114793171537
1104	3	-0.34315709098685687
1104	4	-0.3227744088281739
1104	5	-0.11367606988500967
1104	6	-0.5193736609590545
1104	7	-0.7756309439633853
1104	8	-0.898922939360407
1104	9	-1.05859220209679
1104	10-14	-0.6245426063687489
1104	15-19	-0.5852361096707028
1104	20-24	-0.8309942867293358
1104	25-29	-0.46156008249323577
1104	30-34	-0.7551463662532818
1104	35-39	-0.9196863267938369
1104	40-44	-0.5809304609896984
1104	45-49	-0.545777092526393
1104	50-54	-0.6640210389293628
1104	55-59	-0.6686617751436117
1104	60-64	-0.9196522781760308
1104	65-69	-1.3087741211265396
1104	70-74	-1.2329433407542574
1104	75-79	-0.9252151816438143
1104	80-84	-0.6816225235557383
1104	85-89	-0.7634741178987277
1104	90-94	-1.0267742914099713
1104	95-99	-1.1482276150397013
1104	100-104	-1.2250262684998035
1104	105-109	-1.1406607513944245
1104	110-114	-1.3265049635170172
1104	115-119	-1.2710178465607775
1104	120-124	-1.165284543466015
1104	125-129	-1.6215515447179847
1104	130-134	-1.7123062545476593
1104	135-139	-1.5433368159674679
1104	140-144	-1.6987787063562116
1104	145-149	-2.1207643151912663
1104	150-154	-1.5022867581444004
1104	155-159	-1.7254550182684767
1104	160-164	-2.227752171112897
1104	165-169	-1.853635869916566
1104	170-174	-1.946565363552807
1104	175-179	-2.07219782224783
1104	180-184	-1.8171938297766772
1104	185-189	-2.1790203823380097
1104	190-194	-2.5895344561174376
1104	195-199	-2.2737643320950838
1104	200-204	-2.7215298155976484
1104	205-209	-2.677611070066085
1104	210-214	-1.8069622510184544
1104	215-219	-2.7905054941840426
1104	220-224	-2.543175093621656
1104	225-229	-2.5709888801681764
1104	230-234	-2.5877477639085704
1104	235-239	-2.6580616102953414
1104	240-244	-3.1214708754491802
1104	245-249	-3.74464263088203
1104	250-254	-4.166499286806584
1104	255-259	-4.6354432803178724
1104	260-264	-4.384468282080167
1104	265-269	-4.630933232693383
1104	270-274	-5.269900024393419
1104	275-279	-4.788730828958457
1104	280-284	-5.121851512937155
1104	285-289	-5.565473882984321
1104	290-294	-5.386883878096775
1104	295-299	-5.191624452868805
1104	300-301	-2.3573116668829996
1201	1	-0.6864195456988824
1201	2	-0.04817715022508651
1201	3	-0.2184434151757415
1201	4	-0.3389806267679347
1201	5	-0.7138760935437212
1201	6	-0.16199805739304196
1201	7	-0.5996423861769813
1201	8	-0.35380469956859883
1201	9	-0.3650800662527658
1201	10-14	-0.5396637497297831
1201	15-19	-0.4160289991522532
1201	20-24	-0.2527065322637583
1201	25-29	-0.27923959297297074
1201	30-34	-0.4634000306652055
1201	35-39	-0.425673056407021
1201	40-44	-0.36458579122613344
1201	45-49	-0.6064560023000212
1201	50-54	-0.6445924798322764
1201	55-59	-0.7206870670143921
1201	60-64	-0.9577670411186858
1201	65-69	-0.8922826128937302
1201	70-74	-1.1490550393234962
1201	75-79	-0.8813514815208094
1201	80-84	-1.0519498935896188
1201	85-89	-0.9358655557796176
1201	90-94	-0.8472256282619028
1201	95-99	-1.518637212633763
1201	100-104	-1.0255035330547386
1201	105-109	-1.3497876187737141
1201	110-114	-1.2208464364992686
1201	115-119	-1.5937794431087795
1201	120-124	-1.2078722326556495
1201	125-129	-1.292762031462452
1201	130-134	-1.2635598912381916
1201	135-139	-1.3882208438612018
1201	140-144	-1.2696896658594063
1201	145-149	-1.526462732054739
1201	150-154	-1.855643269996058
1201	155-159	-1.5824894799145923
1201	160-164	-1.9581750697396778
1201	165-169	-1.8238667804387347
1201	170-174	-1.989535275212038
1201	175-179	-2.130059083271547
1201	180-184	-2.006702149170991
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2102	210-214	2.6476077741868167
2102	215-219	2.58620427895265
2102	220-224	2.3979252014935355
2102	225-229	2.0778328614518706
2102	230-234	2.2381435037728785
2102	235-239	2.3374898200755894
2102	240-244	2.503948794137248
2102	245-249	2.7185417576311117
2102	250-254	2.6282828398149967
2102	255-259	3.1827781982907233
2102	260-264	2.8393771100581517
2102	265-269	3.0914646578038365
2102	270-274	3.205879240878037
2102	275-279	3.201611704089057
2102	280-284	3.532824264126077
2102	285-289	3.564179024274484
2102	290-294	3.874943236016584
2102	295-299	3.5791661808814474
2102	300-301	5.08510058330673
2103	1	0.6300194937034505
2103	2	0.6720906964952817
2103	3	0.23966573979119943
2103	4	0.33394711616713124
2103	5	0.9714040100293388
2103	6	0.4422806984568197
2103	7	0.5156038651362564
2103	8	0.824434346200924
2103	9	0.6121253713327945
2103	10-14	0.3970166064087195
2103	15-19	0.5307412405726879
2103	20-24	0.5655376072627334
2103	25-29	0.5218300959894933
2103	30-34	0.6485204218302059
2103	35-39	0.5866262442626038
2103	40-44	0.5063193057144346
2103	45-49	0.6061001086165447
2103	50-54	0.5295842947583722
2103	55-59	0.7072967603072513
2103	60-64	0.749078756001218
2103	65-69	0.9539941691253091
2103	70-74	1.0590206878633701
2103	75-79	0.7229209071328526
2103	80-84	0.7891418683196889
2103	85-89	0.943558464554151
2103	90-94	0.8653957309613958
2103	95-99	1.12824669905838
2103	100-104	1.0918920900027231
2103	105-109	1.1019938231137978
2103	110-114	1.0806259482496614
2103	115-119	1.1249350599881538
2103	120-124	1.3102678903645675
2103	125-129	1.209237582759883
2103	130-134	1.1969964858809057
2103	135-139	1.2528108970676648
2103	140-144	1.1835776566292857
2103	145-149	1.5874842768475617
2103	150-154	1.4929251481140184
2103	155-159	1.3269042346721704
2103	160-164	1.4553289997589722
2103	165-169	1.4839550432557438
2103	170-174	1.5243805823931424
2103	175-179	1.313013635056656
2103	180-184	1.4041606642522382
2103	185-189	1.435904718448345
2103	190-194	1.528386597995972
2103	195-199	1.4008524819147148
2103	200-204	2.0931219102878202
2103	205-209	1.8013708545918306
2103	210-214	2.501543743250288
2103	215-219	2.2801077732001147
2103	220-224	1.9717295620921718
2103	225-229	2.0934720491541725
2103	230-234	1.9977721798899992
2103	235-239	2.191135224995957
2103	240-244	2.408470698614284
2103	245-249	3.026082538094947
2103	250-254	2.9838455407796225
2103	255-259	3.2812497952896535
2103	260-264	3.1872249128448615
2103	265-269	3.4821919481284986
2103	270-274	3.266843440944708
2103	275-279	3.562957669740907
2103	280-284	3.5371925227373104
2103	285-289	3.294804200544025
2103	290-294	3.476142933934373
2103	295-299	3.632391733426772
2103	300-301	2.092409460869842
2104	1	1.272388714014184
2104	2	0.14136254062321285
2104	3	0.47178529726189566
2104	4	0.14564190690866496
2104	5	0.4972529404373276
2104	6	0.3450470548266793
2104	7	0.7746085031415859
2104	8	0.5615043987211266
2104	9	0.0813204635994964
2104	10-14	0.38232191434815377
2104	15-19	0.4791179387076099
2104	20-24	0.626064125030787
2104	25-29	0.4237675322580401
2104	30-34	0.5227789440279409
2104	35-39	0.622859701495365
2104	40-44	0.5452324993593791
2104	45-49	0.570140765173889
2104	50-54	0.4134455712514651
2104	55-59	0.47150188513867164
2104	60-64	0.6002656687124173
2104	65-69	0.93006732461361
2104	70-74	0.9430003815137944
2104	75-79	0.6577454194844208
2104	80-84	0.8225135775836421
2104	85-89	0.8892031472948432
2104	90-94	0.73206239762807
2104	95-99	0.8959571309711336
2104	100-104	0.9727733553867779
2104	105-109	0.9659054682152721
2104	110-114	0.880322508527847
2104	115-119	0.9664169036256496
2104	120-124	1.1837200437936097
2104	125-129	1.0626755133345753
2104	130-134	1.313606112975446
2104	135-139	1.0569888605327016
2104	140-144	1.0791153408331375
2104	145-149	1.316643547216593
2104	150-154	1.3706862148285666
2104	155-159	1.5056260628126026
2104	160-164	1.3850765050208729
2104	165-169	1.381201509836071
2104	170-174	1.4803127857829708
2104	175-179	1.312108178668332
2104	180-184	1.6622450158486117
2104	185-189	1.7059971464325479
2104	190-194	1.632974479660259
2104	195-199	1.5667914156277298
2104	200-204	1.8883283649627103
2104	205-209	2.2594787489123576
2104	210-214	2.6969242210227904
2104	215-219	2.5248791701309727
2104	220-224	1.880699661759948
2104	225-229	2.4465490939255616
2104	230-234	2.19658565876993
2104	235-239	2.4000420435373258
2104	240-244	2.144097972779768
2104	245-249	2.454353381315954
2104	250-254	2.99296118703813
2104	255-259	3.059032108214822
2104	260-264	2.826830355021734
2104	265-269	2.9556049119762484
2104	270-274	3.453861391937817
2104	275-279	3.3089590803385924
2104	280-284	3.495070255770326
2104	285-289	3.3401141237049217
2104	290-294	4.230440363723165
2104	295-299	4.042518526787536
2104	300-301	2.3859026677765307
2201	1	0.42483230831824415
2201	2	0.42209903066202514
2201	3	0.22486425964318357
2201	4	0.1115915472769089
2201	5	0.4860221385088508
2201	6	0.7459777348271217
2201	7	0.7273250372534648
2201	8	0.5704756169946705
2201	9	0.35179933872953484
2201	10-14	0.4479617009181709
2201	15-19	0.45863402985160917
2201	20-24	0.4472057740794142
2201	25-29	0.40953886686658336
2201	30-34	0.48641754487584166
2201	35-39	0.45436635160666583
2201	40-44	0.5101530224194306
2201	45-49	0.5580486368026882
2201	50-54	0.38994366402862823
2201	55-59	-0.13837193574344298
2201	60-64	-0.4441349403684214
2201	65-69	0.3431269247164863
2201	70-74	0.4193547718132855
2201	75-79	0.1859467984835632
2201	80-84	0.31887353713874944
2201	85-89	0.29965690048469895
2201	90-94	0.46032326719328154
2201	95-99	0.7060572627234407
2201	100-104	0.360562957089428
2201	105-109	0.6393527166190474
2201	110-114	0.42274756173551253
2201	115-119	0.6118104362675041
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2201	125-129	0.7464753913089481
2201	130-134	0.7506701764287733
2201	135-139	1.0092459961043936
2201	140-144	0.8930117104342052
2201	145-149	0.906426061999106
2201	150-154	1.0723271414695006
2201	155-159	1.174534348676076
2201	160-164	1.2341033190636317
2201	165-169	1.2360803038217476
2201	170-174	1.5184326270028023
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2201	185-189	1.4621641510706986
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2201	240-244	3.1031424996814323
2201	245-249	3.2906551747606017
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2201	255-259	3.439613431653285
2201	260-264	3.656406731026685
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2201	270-274	4.042915195277331
2201	275-279	4.136097540285309
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2201	290-294	4.561764216070756
2201	295-299	5.1016322223576935
2201	300-301	7.084206300393955
2202	1	-0.029599010519852698
2202	2	0.2992205281225111
2202	3	0.19041810316313246
2202	4	0.3145590756681713
2202	5	0.9040213395589021
2202	6	0.5369610139459979
2202	7	0.3828634724529181
2202	8	0.2699677130969391
2202	9	0.31690805299079017
2202	10-14	0.30314403271067647
2202	15-19	0.5193702051640656
2202	20-24	0.4315509204584629
2202	25-29	0.39550017486178746
2202	30-34	0.5868501192067157
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2202	40-44	0.6637819672348186
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2202	75-79	0.7220012897473751
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2202	90-94	0.855191649328745
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2203	4	0.12748935698915176
2203	5	0.6988375781284191
2203	6	0.24089251840170078
2203	7	0.614443646021563
2203	8	0.763089036412218
2203	9	0.10822962093300958
2203	10-14	0.423344393826639
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2203	25-29	0.4960534977111024
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2204	1	1.213725809540513
2204	2	0.5055822648301955
2204	3	0.3022476829170202
2204	4	0.3588126164157899
2204	5	0.49894820280478314
2204	6	0.26430022917701734
2204	7	0.9326661193068801
2204	8	0.6796380172529624
2204	9	0.474291382454453
2204	10-14	0.3928143368666994
2204	15-19	0.4192262534575306
2204	20-24	0.5295843544031982
2204	25-29	0.5506973804018784
2204	30-34	0.7334375733391667
2204	35-39	0.5661369788412145
2204	40-44	0.581498782424994
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2204	55-59	0.5291333769586046
2204	60-64	0.8138332726900046
2204	65-69	0.9005015095145126
2204	70-74	0.9635250693359971
2204	75-79	0.7063145568426492
2204	80-84	0.732906520646992
2204	85-89	0.7909762408145369
2204	90-94	0.8145911332602438
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2204	100-104	1.015672674957763
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2204	135-139	1.1505803966997412
2204	140-144	1.0846860083585064
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2204	150-154	1.4843311097234704
2204	155-159	1.5842108976474378
2204	160-164	1.317067648706928
2204	165-169	1.267253769723073
2204	170-174	1.7855981588546186
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2204	185-189	1.534956659133865
2204	190-194	1.9196676935311707
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2204	210-214	2.5729155931814063
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2204	250-254	2.560488897422978
2204	255-259	2.5384479304877843
2204	260-264	2.963108362728306
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2204	275-279	3.3120607405831066
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2204	295-299	3.461882700125976
2204	300-301	3.555557646828248
2301	1	-1.7391157306297949
2301	2	0.688570047133048
2301	3	0.7313480161269439
2301	4	0.19415137983674668
2301	5	0.5000589025456179
2301	6	0.6333764972258891
2301	7	0.3293247642531938
2301	8	0.3413373878564414
2301	9	0.7144802014103959
2301	10-14	0.5031623561188283
2301	15-19	0.5892373604549377
2301	20-24	0.6762966831703281
2301	25-29	0.5846527172346683
2301	30-34	0.7061721531050296
2301	35-39	0.6429824561549751
2301	40-44	0.6956463740144869
2301	45-49	0.7257779069048382
2301	50-54	0.6047333689878229
2301	55-59	0.8113746257209016
2301	60-64	0.7640924607615247
2301	65-69	1.0116543938125915
2301	70-74	0.962253664222235
2301	75-79	0.9105158315622575
2301	80-84	1.027450477337588
2301	85-89	0.9053624947865231
2301	90-94	1.0553843543971198
2301	95-99	0.8488788305309143
2301	100-104	1.063423645888676
2301	105-109	1.10395836353187
2301	110-114	0.9291850423436401
2301	115-119	1.0572339547285665
2301	120-124	0.9623145418122441
2301	125-129	1.072651004092073
2301	130-134	1.1638454789419548
2301	135-139	1.194453642812757
2301	140-144	1.3307919052109831
2301	145-149	1.4782071819030946
2301	150-154	1.4011423535611627
2301	155-159	1.4996095514206687
2301	160-164	1.3883418239060212
2301	165-169	1.4140470006673347
2301	170-174	1.7585102752259019
2301	175-179	1.6920028972429932
2301	180-184	1.4664354479652317
2301	185-189	1.8681579383090856
2301	190-194	1.8605054214254686
2301	195-199	1.900440339114656
2301	200-204	2.470640274440335
2301	205-209	2.3097062882039268
2301	210-214	-3.2668993773820922
2301	215-219	2.004536688310669
2301	220-224	2.371241533681136
2301	225-229	2.6167484840264343
2301	230-234	2.324354835406094
2301	235-239	2.768970028524425
2301	240-244	2.9805980900680638
2301	245-249	2.802943576014524
2301	250-254	3.0286731269865186
2301	255-259	3.0140543929908006
2301	260-264	3.3005812346839427
2301	265-269	3.596820018708595
2301	270-274	3.361912060481096
2301	275-279	3.568419554183304
2301	280-284	3.427774923844453
2301	285-289	4.241255590233802
2301	290-294	4.247913202646171
2301	295-299	4.163009459029507
2301	300-301	1.6650270762293964
2302	1	1.1453710288569638
2302	2	0.3816949902579907
2302	3	0.22823126301018704
2302	4	0.148628584313947
2302	5	0.38164503413175055
2302	6	0.33520332405271347
2302	7	0.7845640944925236
2302	8	0.4021254486445045
2302	9	0.2103851973153894
2302	10-14	0.4425744955309625
2302	15-19	0.47412224533982794
2302	20-24	0.5313808582544937
2302	25-29	0.36778802511574327
2302	30-34	0.6237912822495844
2302	35-39	0.6159825132228249
2302	40-44	0.4636883759547885
2302	45-49	0.5095637883178483
2302	50-54	0.47344534753031553
2302	55-59	0.6873547661078305
2302	60-64	0.7498254973420231
2302	65-69	0.9840496972006321
2302	70-74	1.0342659826631184
2302	75-79	0.6906155820057407
2302	80-84	0.8829158403114832
2302	85-89	0.8099976760428618
2302	90-94	0.851431766997429
2302	95-99	1.2454820036699203
2302	100-104	1.0203418268682967
2302	105-109	1.2673404316067653
2302	110-114	1.1009575551319486
2302	115-119	1.3090275049131463
2302	120-124	1.4033885823499652
2302	125-129	1.2481797821561997
2302	130-134	1.2605996755011297
2302	135-139	1.3082192855886454
2302	140-144	1.4222300784979787
2302	145-149	1.6513238512126662
2302	150-154	1.4676143285092635
2302	155-159	1.4932386754252107
2302	160-164	1.7227377517997091
2302	165-169	1.6219144226440356
2302	170-174	1.8199132284412514
2302	175-179	1.8235112889251965
2302	180-184	1.6706834805233228
2302	185-189	1.9308327991557341
2302	190-194	2.1694332528246996
2302	195-199	1.8472902818408876
2302	200-204	2.4204428774343683
2302	205-209	2.42978770582846
2302	210-214	2.89774621648764
2302	215-219	2.6455424354371075
2302	220-224	2.460858391918684
2302	225-229	2.7015377560570855
2302	230-234	2.381931463758587
2302	235-239	2.629152020861582
2302	240-244	3.0124228027117397
2302	245-249	3.425314265669698
2302	250-254	3.6627232992000636
2302	255-259	3.39012552118977
2302	260-264	3.763515272891322
2302	265-269	3.9304016599437226
2302	270-274	3.9670520340483186
2302	275-279	4.343370893844483
2302	280-284	4.40812816185959
2302	285-289	4.363658569928617
2302	290-294	4.855066873197657
2302	295-299	4.826423186597143
2302	300-301	0.18739057464657805
2303	1	0.5602314979547245
2303	2	-0.28722014984359134
2303	3	0.5192316624851685
2303	4	0.13489805871901694
2303	5	0.4124959158300854
2303	6	0.35729999699684356
2303	7	1.0438804216054578
2303	8	0.42251008597829554
2303	9	0.4016538157026517
2303	10-14	0.38449996491406324
2303	15-19	0.4780142729606709
2303	20-24	0.5210424458821876
2303	25-29	0.4368561070651751
2303	30-34	0.7061721531050296
2303	35-39	0.6260333036126084
2303	40-44	0.5675107807941373
2303	45-49	0.7291677374133201
2303	50-54	0.58575031814037
2303	55-59	0.6473068291107325
2303	60-64	0.6942619522869435
2303	65-69	0.8224418952538457
2303	70-74	1.1346530646603732
2303	75-79	0.7348826456795834
2303	80-84	0.9158521664681558
2303	85-89	0.6040563109489554
2303	90-94	0.4543222846337116
2303	95-99	0.715759145218243
2303	100-104	0.948738590979346
2303	105-109	1.107315610867655
2303	110-114	0.9499600417632053
2303	115-119	1.0442646714521118
2303	120-124	1.1841575452252222
2303	125-129	1.2194086832729525
2303	130-134	1.1583847280887092
2303	135-139	1.2619626427659973
2303	140-144	1.2040258104894015
2303	145-149	1.3253482357158006
2303	150-154	1.3596069849137251
2303	155-159	1.497503609756528
2303	160-164	1.5330470301502253
2303	165-169	1.3373783401698098
2303	170-174	1.5319169383545628
2303	175-179	1.7312343296272203
2303	180-184	1.4929833889645607
2303	185-189	1.541869840475261
2303	190-194	1.7187899823319164
2303	195-199	1.8983475675002488
2303	200-204	2.017535341871124
2303	205-209	2.284418136432066
2303	210-214	2.376038219563384
2303	215-219	2.1208163685397565
2303	220-224	1.9297357467822032
2303	225-229	2.573183277010564
2303	230-234	2.354773639844712
2303	235-239	2.331828875917335
2303	240-244	2.5992235524233855
2303	245-249	2.9178964456004906
2303	250-254	2.9249528072156075
2303	255-259	2.715937223201994
2303	260-264	2.9435593995807317
2303	265-269	2.704706905714918
2303	270-274	3.2715720055950435
2303	275-279	3.3977287058358563
2303	280-284	3.558064865378878
2303	285-289	3.493077568518011
2303	290-294	3.805529238918691
2303	295-299	4.057419344570793
2303	300-301	1.929507362967744
2304	1	1.6449411986398985
2304	2	0.8339121977943478
2304	3	0.5326226950611925
2304	4	0.2873562868714359
2304	5	0.3027711893004579
2304	6	0.5617596676303336
2304	7	0.6559732212207976
2304	8	0.8421999525487962
2304	9	0.24649093554878476
2304	10-14	0.45537627216253185
2304	15-19	0.3277077457338393
2304	20-24	0.547076825014301
2304	25-29	0.5086147318998897
2304	30-34	0.7626837530569475
2304	35-39	0.7505122073269916
2304	40-44	0.6016567117954637
2304	45-49	0.537130232905568
2304	50-54	0.6971146648166524
2304	55-59	0.6504538419728689
2304	60-64	0.7943845631776796
2304	65-69	0.8677502255820144
2304	70-74	0.948445587800169
2304	75-79	0.7133920819098947
2304	80-84	0.6659904042371139
2304	85-89	0.7194379673980507
2304	90-94	1.016459560748629
2304	95-99	1.033583402941531
2304	100-104	0.9800600557354571
2304	105-109	1.1803507823830692
2304	110-114	1.0362290625925041
2304	115-119	1.0404095951157757
2304	120-124	1.2729579047408457
2304	125-129	1.1820263456926483
2304	130-134	1.2722127192951582
2304	135-139	1.4016949676764163
2304	140-144	1.1934666528820337
2304	145-149	1.4197810344288087
2304	150-154	1.220573201459331
2304	155-159	1.405219695591292
2304	160-164	1.6280378232536172
2304	165-169	1.4064799601992668
2304	170-174	1.702237725250285
2304	175-179	1.8103890503390403
2304	180-184	1.5612038536210093
2304	185-189	1.596768505823757
2304	190-194	1.7485040678396153
2304	195-199	1.5920931492244002
2304	200-204	2.312781587111793
2304	205-209	2.3255090319933274
2304	210-214	2.993669155555736
2304	215-219	2.6656589228923053
2304	220-224	2.5520770299115156
2304	225-229	2.6622904442291286
2304	230-234	2.2960443669843933
2304	235-239	2.434662773549757
2304	240-244	3.0566208313855725
2304	245-249	3.5708115774976505
2304	250-254	3.35134044807959
2304	255-259	3.7397006346181882
2304	260-264	3.4259347449791555
2304	265-269	3.8031211846723743
2304	270-274	3.4745044693126594
2304	275-279	3.8282905539352257
2304	280-284	3.8675070002522958
2304	285-289	3.9244160963116954
2304	290-294	4.122058576002658
2304	295-299	3.9599820023158543
2304	300-301	4.157149948519077
2401	1	0.22497501574572
2401	2	0.49369802279082364
2401	3	-0.05194355358846536
2401	4	0.14739104334130815
2401	5	0.5265042220372962
2401	6	0.4567387667802123
2401	7	0.49235948771838167
2401	8	0.5278417730363287
2401	9	-0.044782372421714456
2401	10-14	0.2615478939745657
2401	15-19	0.4291872441134359
2401	20-24	0.5245086037001201
2401	25-29	0.20607375214319035
2401	30-34	0.5050631621969615
2401	35-39	0.38779556649283364
2401	40-44	0.41743333102424884
2401	45-49	0.3656634159406522
2401	50-54	0.3568065534080702
2401	55-59	0.3904519632447645
2401	60-64	0.6057132936271685
2401	65-69	0.6483617515789106
2401	70-74	0.7518648729441288
2401	75-79	0.3781894141159867
2401	80-84	0.4693486307177892
2401	85-89	0.6474685906661009
2401	90-94	0.4490601901225517
2401	95-99	0.8893495456084608
2401	100-104	0.7147882666635752
2401	105-109	0.8471352596574029
2401	110-114	0.8259296819408917
2401	115-119	0.6685908651409491
2401	120-124	0.9809396027581236
2401	125-129	0.8256200605931525
2401	130-134	1.2715792673378594
2401	135-139	0.9602192855886393
2401	140-144	1.0578248972473894
2401	145-149	1.3623528019258444
2401	150-154	1.216803331945691
2401	155-159	1.1572386754252193
2401	160-164	1.3290676487069284
2401	165-169	1.2580037697230715
2401	170-174	1.2574508165068536
2401	175-179	1.65920414828458
2401	180-184	1.3342998880213912
2401	185-189	1.5430716511756408
2401	190-194	1.924382049399565
2401	195-199	1.7921075823628847
2401	200-204	1.986225705717203
2401	205-209	2.156702688993434
2401	210-214	2.1695805508219763
2401	215-219	2.197682075076745
2401	220-224	2.3062788123391087
2401	225-229	2.914525744045079
2401	230-234	2.7299419742690993
2401	235-239	2.8960063752159435
2401	240-244	3.0985151450540833
2401	245-249	3.617375076480503
2401	250-254	3.8214131083471834
2401	255-259	3.412484029523892
2401	260-264	3.7612314787327854
2401	265-269	3.9822554365928724
2401	270-274	4.008493007295826
2401	275-279	4.140786591003874
2401	280-284	4.044490294972444
2401	285-289	4.253856408468749
2401	290-294	4.657556685989245
2401	295-299	4.529159796675405
2401	300-301	2.3590607919948603
2402	1	1.051094934580874
2402	2	0.8126714212344197
2402	3	0.6120696468485747
2402	4	0.1385275742129366
2402	5	0.44561809810481634
2402	6	0.6348666237160145
2402	7	0.9428132527416793
2402	8	0.5334385799576324
2402	9	0.462910449840642
2402	10-14	0.5570526100090731
2402	15-19	0.624290595508171
2402	20-24	0.6270037538773892
2402	25-29	0.551626408954121
2402	30-34	0.7618384202967192
2402	35-39	0.7270936243339392
2402	40-44	0.6785031907696037
2402	45-49	0.6314493102033651
2402	50-54	0.725970600055561
2402	55-59	0.6637857425388063
2402	60-64	0.722216069732589
2402	65-69	1.058351271502211
2402	70-74	1.182366355763488
2402	75-79	0.9314950978852607
2402	80-84	0.9768006341962732
2402	85-89	0.8751582912034763
2402	90-94	1.006136471702142
2402	95-99	1.4558127890007029
2402	100-104	1.0301925867190533
2402	105-109	1.2620328512991854
2402	110-114	1.1524421654301094
2402	115-119	1.096024195481263
2402	120-124	1.381325703144224
2402	125-129	1.29027391710747
2402	130-134	1.4352549430135362
2402	135-139	1.446282348651696
2402	140-144	1.4920298140285801
2402	145-149	1.7211550618128513
2402	150-154	1.8250632189513354
2402	155-159	1.644199127402615
2402	160-164	1.5357682136786757
2402	165-169	1.5791676115309876
2402	170-174	1.7237026162914475
2402	175-179	1.7771929244310485
2402	180-184	1.653431456526576
2402	185-189	1.9552672951700067
2402	190-194	1.8308045269334094
2402	195-199	1.9852187187523747
2402	200-204	2.4215210318599816
2402	205-209	2.352219438408497
2402	210-214	2.9892198511392394
2402	215-219	2.765132830917331
2402	220-224	2.5047190322200024
2402	225-229	2.6308221251719637
2402	230-234	2.588235606884769
2402	235-239	3.0085258438371056
2402	240-244	3.5408903168360304
2402	245-249	3.534106638551048
2402	250-254	3.693337066203352
2402	255-259	4.140763919583435
2402	260-264	4.217518698155597
2402	265-269	4.349035097609825
2402	270-274	4.279679447973788
2402	275-279	4.3760408282920125
2402	280-284	4.515676735650413
2402	285-289	4.309449628807734
2402	290-294	5.046031262260431
2402	295-299	4.942197430093248
2402	300-301	2.4585939584600354
2404	1	0.11331578251600405
2404	2	1.0908958423159802
2404	3	0.3396859458934429
2404	4	-0.026799162542367583
2404	5	0.6100515482525495
2404	6	0.7528148130927761
2404	7	0.4163582262866541
2404	8	0.4940308976928094
2404	9	0.4574476586635683
2404	10-14	0.4854385098235454
2404	15-19	0.7170549739868406
2404	20-24	0.5474531457553624
2404	25-29	0.5557561416552801
2404	30-34	0.803905348077933
2404	35-39	0.7722664409353328
2404	40-44	0.49069998868068154
2404	45-49	0.7921309565993013
2404	50-54	0.7118751518304194
2404	55-59	0.8667804219839965
2404	60-64	0.8133664817021113
2404	65-69	1.1689774421966348
2404	70-74	1.1818515375012417
2404	75-79	0.8296456213339738
2404	80-84	0.9907546069131428
2404	85-89	1.126961547130449
2404	90-94	0.9581792361160382
2404	95-99	1.1130379616897912
2404	100-104	1.048423000825764
2404	105-109	1.2744942348945862
2404	110-114	1.0431711793550065
2404	115-119	1.1079054065892748
2404	120-124	1.4525154495917114
2404	125-129	1.1739738239108277
2404	130-134	1.493717198372341
2404	135-139	1.4608629637495518
2404	140-144	1.4071812190864712
2404	145-149	1.7219849858338918
2404	150-154	1.6474010330951216
2404	155-159	1.5817444225516581
2404	160-164	1.7654584533046211
2404	165-169	1.6031302065046766
2404	170-174	2.0728306480177707
2404	175-179	1.9485353676895087
2404	180-184	1.699656303228302
2404	185-189	1.9200224740777223
2404	190-194	2.2490884252384937
2404	195-199	1.9167347262853305
2404	200-204	2.5572484329899297
2404	205-209	2.6513154836062256
2404	210-214	3.0664962164876357
2404	215-219	3.0392924354371047
2404	220-224	2.780302836363127
2404	225-229	2.916815533834857
2404	230-234	2.790959241536367
2404	235-239	3.2916520208615836
2404	240-244	3.915200580489522
2404	245-249	3.901008710114141
2404	250-254	3.812873318557397
2404	255-259	3.9754720175118727
2404	260-264	3.9916971350670813
2404	265-269	4.01681522943657
2404	270-274	4.6257434781056155
2404	275-279	4.5084913932637605
2404	280-284	4.34481515372951
2404	285-289	4.779981643873647
2404	290-294	4.851739830998664
2404	295-299	5.200654101864359
2404	300-301	3.503917395268715
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	7.0
16	240.0
17	559.0
18	591.0
19	560.0
20	526.0
21	631.0
22	770.0
23	1010.0
24	1224.0
25	1806.0
26	2522.0
27	3824.0
28	6016.0
29	8648.0
30	10776.0
31	12657.0
32	16958.0
33	24417.0
34	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	99.33111080341261	0.18426406211722604	0.07455771299540938	0.4100674214747515
2	0.4856902446558097	98.87204831339803	0.119292340792655	0.5229691011535144
3	0.42710918444513085	0.46332293075718683	0.19704538434501048	98.91252250045267
4	0.17361296026073897	0.31314239458071935	99.12660964776806	0.38663499739048
5	0.2364544612140126	98.90719694952442	0.46119271038588944	0.3951558788756697
6	0.24284512232790484	0.6827356290008201	0.20663137601584886	98.86778787265543
7	0.3887652177617774	31.870226974980564	37.23412186990744	30.50688593735022
8	34.518090896503246	33.05143417086497	0.502732007626189	31.927742925005592
9	99.19690692002088	0.18532917230287474	0.3557468020066676	0.26201710566958153
10-14	39.92948970571006	19.964425319799332	39.873251887907806	0.232833086582807
15-19	0.26351668015849344	39.850666780282054	39.7673725022368	20.118444037322654
20-24	19.405010727533433	1.7626392075900228	37.64245674364498	41.189893321231565
25-29	20.883356986690192	0.6467381807798362	42.590958877253996	35.878945955275974
30-34	1.419998039324334	35.150865467812956	2.6292234445661578	60.79991304829656
35-39	19.73463776456295	2.046166634695313	55.842871453844026	22.376324146897712
40-44	39.07797986172256	17.85326730013623	21.760681537638284	21.308071300502924
45-49	2.697988237351314	20.032754583722507	21.116683939591677	56.1525732393345
50-54	37.194339115636105	3.8600816375276708	21.60003582838205	37.34554341845418
55-59	36.891748058435695	22.48353856359436	38.63824940329293	1.9864639746770103
60-64	21.93512848761241	34.66774273440769	4.682389211993885	38.71473956598601
65-69	32.69873636332547	39.71670770493485	2.5076091788926504	25.07694675284703
70-74	41.19615823027094	19.085879507353553	2.1471480783666275	37.570814184008874
75-79	1.7979624233555405	1.4023850172650605	40.51534172838872	56.28431083099068
80-84	21.741140215716488	19.201335387776066	2.8785738743365865	56.17895052217086
85-89	58.29400319778082	0.7189655799776968	40.04482030141332	0.9422109208281695
90-94	2.929656968240814	1.309469509596709	73.80011692074444	21.960756601418037
95-99	2.348957351656072	37.795788283271364	20.95256249892908	38.902691866143485
100-104	2.0995012720028634	20.342186987635746	56.9180024304149	20.640309309946485
105-109	20.257156824068197	38.38527641035317	3.4000274437831255	37.95753932179551
110-114	1.349817757440409	20.846446430984884	40.20019865404377	37.603537157530944
115-119	20.679167364250237	1.1253632800594127	21.331032380756138	56.86443697493421
120-124	1.5759493806776583	54.4600011594178	3.9894707687154725	39.974578691189066
125-129	54.496353437389935	25.412976436934652	19.119541967683467	0.9711281579919424
130-134	2.063527869979483	18.312010570075085	40.088513636900736	39.535947923044695
135-139	7.910611402528163	2.1175509502106804	38.07313612520423	51.898701522056925
140-144	2.325351191295965	2.0989705274755543	54.33759984850571	41.238078432722766
145-149	19.16815218608176	22.349316661069057	19.669138226505112	38.81339292634407
150-154	2.454134418411286	37.592867436244845	21.322514146868947	38.630483998474915
155-159	24.944838047956424	36.98630432159418	19.457085725121097	18.6117719053283
160-164	24.303382217279225	27.986490098198967	23.29013958220176	24.419988102320048
165-169	18.19934193787652	5.170488113052141	43.44525276744016	33.184917181631185
170-174	23.18025104746946	33.268751968724786	18.926511009756243	24.62448597404951
175-179	10.31331085427613	8.306169939945516	48.16253135483091	33.217987850947445
180-184	40.65260302610153	2.74059580423205	33.75165753134732	22.8551436383191
185-189	29.109788116822077	30.538838046050287	20.313124655595296	20.038249181532343
190-194	44.38420650699527	27.348937026356378	5.15467039920734	23.11218606744101
195-199	19.70878137170642	2.776015812227632	58.81265411185029	18.702548704215655
200-204	2.505836845547754	37.214167832591905	18.900333967192378	41.37966135466796
205-209	22.008348794063078	36.79169628769095	3.3024552216808556	37.89749969656512
210-214	8.057840275935261	24.54939418059609	58.4653312107544	8.927434332714247
215-219	8.434514785162275	24.749888139289393	15.71697285368619	51.098624221862146
220-224	34.35976676511561	4.615147227890824	22.17078826523405	38.85429774175952
225-229	15.355317251430186	9.078807125921736	17.67798490418289	57.88789071846519
230-234	18.20856545961003	1.5213701253481895	57.5202385097493	22.74982590529248
235-239	19.579811986768956	19.77884382968623	2.199802710230106	58.44154147331471
240-244	4.033212382726946	54.80653351203513	21.40803940156691	19.752214703671015
245-249	2.6051592926143177	37.2631326457184	21.386423602782443	38.745284458884846
250-254	37.3003090299602	40.45739053006495	7.586510929534185	14.655789510440673
255-259	19.670358713149614	19.71251400625995	21.950239280433173	38.666888000157265
260-264	19.47531080065045	19.17740553583606	20.543005018272105	40.80427864524138
265-269	2.483617228439835	18.991105714004398	21.780388810484975	56.74488824707079
270-274	19.29852995286738	21.231361589543216	57.47332363814763	1.9967848194417677
275-279	20.920216593825145	19.576817088796446	3.345663735453636	56.157302581924775
280-284	56.583754995682675	1.333427076764885	20.63109835809844	21.451719569453996
285-289	40.15872247548977	2.319451533979899	19.402249485153046	38.11957650537729
290-294	21.560762509928516	35.82472862059836	18.878960374194687	23.73554849527844
295-299	37.523222438060486	19.577769798452653	39.96913339325261	2.9298743702342427
300-301	11.272546174999459	1.5081306974427822	1.0144425437931708	86.20488058376459
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.25
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.25
16	0.0
17	0.25
18	1.0
19	1.25
20	1.0
21	1.0
22	0.5
23	0.3333333333333333
24	0.5
25	1.0
26	2.0
27	2.5
28	2.3333333333333335
29	2.0833333333333335
30	3.8333333333333335
31	5.833333333333334
32	9.916666666666666
33	11.916666666666668
34	10.583333333333336
35	8.083333333333332
36	12.833333333333334
37	20.999999999999996
38	26.58333333333333
39	31.24999999999999
40	35.583333333333336
41	60.08333333333337
42	113.66666666666644
43	805.1666666666858
44	964.7500000000364
45	221.91666666666782
46	115.16666666666654
47	96.66666666666657
48	162.7500000000001
49	258.0833333333334
50	371.2499999999998
51	1010.7500000000057
52	7652.6666666666515
53	10360.00000000001
54	5485.999999999997
55	4169.833333333336
56	817.0
57	249.58333333333337
58	96.66666666666666
59	63.83333333333334
60	42.916666666666664
61	22.166666666666664
62	12.25
63	18.0
64	23.0
65	15.333333333333334
66	7.833333333333333
67	4.333333333333333
68	2.8333333333333335
69	1.8333333333333333
70	0.75
71	0.5833333333333333
72	0.8333333333333333
73	0.25
74	0.5833333333333333
75	0.8333333333333333
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.3333333333333333
84	0.3333333333333333
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.25
91	0.8333333333333333
92	1.8333333333333333
93	1.25
94	1.8333333333333333
95	1.3333333333333333
96	1.0
97	4.083333333333333
98	8.25
99	15.083333333333334
100	44.83333333333335
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	1.854283079260023
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	2.19017269511701E-4
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
10-19	12.0
20-29	24.0
30-39	23.0
40-49	46.0
50-59	89.0
60-69	169.0
70-79	65.0
80-89	55.0
90-99	72.0
100-109	66.0
110-119	108.0
120-129	40.0
130-139	120.0
140-149	144.0
150-159	52.0
160-169	67.0
170-179	102.0
180-189	112.0
190-199	162.0
200-209	130.0
210-219	178.0
220-229	132.0
230-239	122.0
240-249	120.0
250-259	137.0
260-269	182.0
270-279	257.0
280-289	345.0
290-299	4182.0
300-302	86574.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.368570728641878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.33766068473972	5.9699425905609935
2	8.323787705231005	1.3931641228285065
3	4.009163802978236	1.0065291254380266
4	2.698230876925035	0.9032134374301022
5	1.7054855542828051	0.7136238243846326
6	1.794578083237877	0.9010832170588047
7	1.3618429425989562	0.7977675290508803
8	1.1709303805523736	0.7839210966374471
9	0.7509227440498918	0.5655735085794625
>10	5.638284332442408	8.411175136067826
>50	0.6491027109583811	3.5787702237796504
>100	0.4454626447753595	8.098032741487108
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.11454753722794961	66.87720344669657
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	8014	8.535793027788726	No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7900	8.414370466624773	No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7611	8.106553622972298	No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7025	7.482399054182156	No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6770	7.210795956841736	No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6676	7.110675599390757	No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6526	6.950909071543451	No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6257	6.66439443160395	No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6010	6.401312215748719	No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA	396	0.42178363351688736	No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA	374	0.39835120943261576	No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA	353	0.375983895533993	No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA	324	0.34509570015018054	No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA	308	0.32805393717980125	No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA	299	0.3184679455089629	No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA	299	0.3184679455089629	No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA	298	0.3174028353233142	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	291	0.30994706402377326	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	284	0.3024912927242323	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	275	0.29290530105339396	No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA	267	0.2843844195682043	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	245	0.26095199548393283	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	244	0.2598868852982841	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	244	0.2598868852982841	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	243	0.25882177511263543	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	224	0.23858468158531	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	201	0.2140871473153898	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	185	0.19704538434501048	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	180	0.19171983341676696	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	175	0.18639428248852344	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	172	0.18319895193157731	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	169	0.1800036213746312	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	156	0.16615718896119805	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	149	0.1587014176616571	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	146	0.15550608710471098	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	143	0.15231075654776488	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	142	0.15124564636211615	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	132	0.1405945445056291	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	129	0.13739921394868299	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	123	0.13100855283479076	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	112	0.119292340792655	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	111	0.1182272306070063	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	108	0.11503190005006018	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	102	0.10864123893616795	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	99	0.10544590837922184	No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	94	0.1001203574509783	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	4.260440742594821E-4	0.0
90-94	0.0	0.0	0.0	0.0021302203712974107	0.0
95-99	0.0	0.0	0.0	0.0021302203712974107	0.0
100-104	0.0	0.0	0.0	0.0021302203712974107	0.0
105-109	0.0	0.0	0.0	0.0021302203712974107	0.0
110-114	0.0	0.0	0.0	0.0021302203712974107	0.0
115-119	0.0	0.0	0.0	0.0021302203712974107	0.0
120-124	0.0	0.0	0.0	0.0021302203712974107	0.0
125-129	0.0	0.0	0.0	0.0021302203712974107	0.0
130-134	0.0	0.0	0.0	0.0021302203712974107	0.0
135-139	0.0	0.0	0.0	0.0021302203712974107	0.0
140-144	0.0	0.0	0.0	0.0021302203712974107	0.0
145-149	0.0	0.0	0.0	0.0021302203712974107	0.0
150-154	0.0	0.0	0.0	0.0021302203712974107	0.0
155-159	0.0	0.0	0.0	0.0021302203712974107	0.0
160-164	0.0	0.0	0.0	0.0021302203712974107	0.0
165-169	0.0	0.0	0.0	0.004686484816854302	0.0
170-174	0.0	8.520881485189642E-4	0.0	0.005325550928243526	0.0
175-179	0.0	0.0010651101856487054	0.0	0.005325550928243526	0.0
180-184	0.0	0.0010651101856487054	0.0	0.005325550928243526	0.0
185-189	0.0	0.0010651101856487054	0.0	0.005538572965373268	0.0
190-194	0.0	0.0010651101856487054	0.0	0.006390661113892232	0.0
195-199	0.0	0.0010651101856487054	0.0	0.006390661113892232	0.0
200-204	0.0	0.0010651101856487054	0.0	0.006390661113892232	0.0
205-209	0.0	0.0010651101856487054	0.0	0.006390661113892232	0.0
210-214	0.0	0.0010651101856487054	0.0	0.007029727225281455	0.0
215-219	0.0	0.0010651101856487054	0.0	0.007455771299540936	0.0
220-224	0.0	0.0010651101856487054	0.0	0.007455771299540936	0.0
225-229	0.0	0.0010651101856487054	0.0	0.007455771299540936	0.0
230-234	0.0	0.0010651101856487054	0.0	0.007455771299540936	0.0
235-239	0.0	0.0010651101856487054	0.0	0.007455771299540936	0.0
240-244	0.0	0.0010651101856487054	0.0	0.007455771299540936	0.0
245-249	0.0	0.0010651101856487054	0.0	0.007455771299540936	0.0
250-254	0.0	0.0010651101856487054	0.0	0.007455771299540936	0.0
255-259	0.0	0.0010651101856487054	0.0	0.007455771299540936	0.0
260-264	0.0	0.0010651101856487054	0.0	0.007455771299540936	0.0
265-269	0.0	0.0010651101856487054	0.0	0.007455771299540936	0.0
270-274	0.0	0.0010651101856487054	0.0	0.007455771299540936	0.0
275-279	0.0	0.0010651101856487054	0.0	0.007455771299540936	0.0
280-284	0.0	0.0010651101856487054	0.0	0.007455771299540936	0.0
285-289	0.0	0.0010651101856487054	0.0	0.007455771299540936	0.0
290	0.0	0.0010651101856487054	0.0	0.007455771299540937	0.0
>>END_MODULE
