##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	DCMS2_cleaned_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93887
Sequences flagged as poor quality	0
Sequence length	26-301
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16436780384931	34.0	34.0	34.0	20.0	34.0
2	33.057228370274906	34.0	34.0	34.0	34.0	34.0
3	33.58578929990308	34.0	34.0	34.0	34.0	34.0
4	33.44346927689669	34.0	34.0	34.0	34.0	34.0
5	33.236347950195444	34.0	34.0	34.0	34.0	34.0
6	33.04805777157647	34.0	34.0	34.0	34.0	34.0
7	33.5088244378881	34.0	34.0	34.0	34.0	34.0
8	33.462108705145546	34.0	34.0	34.0	34.0	34.0
9	33.38137335307338	34.0	34.0	34.0	34.0	34.0
10-14	33.32158658813255	34.0	34.0	34.0	34.0	34.0
15-19	33.43821615346108	34.0	34.0	34.0	34.0	34.0
20-24	33.66741934453119	34.0	34.0	34.0	34.0	34.0
25-29	33.608808484718885	34.0	34.0	34.0	34.0	34.0
30-34	33.543222026947866	34.0	34.0	34.0	34.0	34.0
35-39	33.576467421982656	34.0	34.0	34.0	34.0	34.0
40-44	33.42111347342802	34.0	34.0	34.0	34.0	34.0
45-49	33.60165945011888	34.0	34.0	34.0	34.0	34.0
50-54	33.54625934482678	34.0	34.0	34.0	34.0	34.0
55-59	33.38580175884618	34.0	34.0	34.0	34.0	34.0
60-64	33.32637110648958	34.0	34.0	34.0	34.0	34.0
65-69	33.521267309026705	34.0	34.0	34.0	34.0	34.0
70-74	33.56141375598214	34.0	34.0	34.0	34.0	34.0
75-79	33.37944456152232	34.0	34.0	34.0	34.0	34.0
80-84	33.600502988328046	34.0	34.0	34.0	34.0	34.0
85-89	33.56289831866506	34.0	34.0	34.0	34.0	34.0
90-94	33.150802300225486	34.0	34.0	34.0	34.0	34.0
95-99	33.33620956541836	34.0	34.0	34.0	34.0	34.0
100-104	33.298300781983606	34.0	34.0	34.0	34.0	34.0
105-109	33.3374028223984	34.0	34.0	34.0	34.0	34.0
110-114	33.21208150956844	34.0	34.0	34.0	34.0	34.0
115-119	33.058300161043334	34.0	34.0	34.0	34.0	34.0
120-124	33.17489883731015	34.0	34.0	34.0	34.0	34.0
125-129	33.288107396651036	34.0	34.0	34.0	34.0	34.0
130-134	32.97316431088242	34.0	34.0	34.0	31.2	34.0
135-139	33.14131896221532	34.0	34.0	34.0	34.0	34.0
140-144	33.25793469049323	34.0	34.0	34.0	34.0	34.0
145-149	33.34679889213173	34.0	34.0	34.0	34.0	34.0
150-154	33.026121935754084	34.0	34.0	34.0	34.0	34.0
155-159	33.13906502927507	34.0	34.0	34.0	34.0	34.0
160-164	33.25979441748234	34.0	34.0	34.0	34.0	34.0
165-169	33.28609019888274	34.0	34.0	34.0	34.0	34.0
170-174	32.87056946035528	34.0	34.0	34.0	31.2	34.0
175-179	33.24934662761139	34.0	34.0	34.0	34.0	34.0
180-184	32.99757662274642	34.0	34.0	34.0	34.0	34.0
185-189	33.17030147473265	34.0	34.0	34.0	34.0	34.0
190-194	33.1189303273099	34.0	34.0	34.0	34.0	34.0
195-199	32.5621396931135	34.0	34.0	34.0	28.4	34.0
200-204	32.95451046042154	34.0	34.0	34.0	34.0	34.0
205-209	32.63539750672336	34.0	34.0	34.0	31.2	34.0
210-214	32.61027044708119	34.0	34.0	34.0	31.2	34.0
215-219	32.91986878100663	34.0	34.0	34.0	34.0	34.0
220-224	32.92650176476431	34.0	34.0	34.0	34.0	34.0
225-229	33.065716664940766	34.0	34.0	34.0	34.0	34.0
230-234	32.77075593276019	34.0	34.0	34.0	31.2	34.0
235-239	32.81518693452275	34.0	34.0	34.0	34.0	34.0
240-244	32.677594752588575	34.0	34.0	34.0	31.2	34.0
245-249	32.86319892311606	34.0	34.0	34.0	34.0	34.0
250-254	32.44914970651658	34.0	34.0	34.0	31.2	34.0
255-259	32.39430951185559	34.0	34.0	34.0	31.2	34.0
260-264	32.66673789500547	34.0	34.0	34.0	34.0	34.0
265-269	32.71562834640362	34.0	34.0	34.0	34.0	34.0
270-274	32.42885841286108	34.0	34.0	34.0	34.0	34.0
275-279	32.497718430253435	34.0	34.0	34.0	31.2	34.0
280-284	32.0064111821362	34.0	34.0	34.0	22.8	34.0
285-289	31.63310333698862	34.0	34.0	34.0	20.0	34.0
290-294	31.780122438259117	34.0	34.0	34.0	20.0	34.0
295-299	31.910674586468406	34.0	34.0	34.0	20.0	34.0
300-301	31.572427579452757	34.0	34.0	34.0	20.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.2666166046003937
1101	2	0.28837870601518745
1101	3	0.15694540312653515
1101	4	0.08589562895323866
1101	5	0.0625849816337336
1101	6	0.25753432222169437
1101	7	-0.1833740976016287
1101	8	0.0792649661777034
1101	9	-0.47564337686065983
1101	10-14	-0.17200478433909439
1101	15-19	0.07959967352513786
1101	20-24	-0.1181630829552347
1101	25-29	-0.026215988870326612
1101	30-34	0.02783298925538702
1101	35-39	0.12031922780420246
1101	40-44	0.01796475602794345
1101	45-49	0.023915195149776025
1101	50-54	0.10787302748956762
1101	55-59	0.00944846037906899
1101	60-64	0.06295312562819078
1101	65-69	0.07767210027409988
1101	70-74	0.012630672023675515
1101	75-79	0.10227373567831677
1101	80-84	0.13729659552656415
1101	85-89	-0.01238635145789857
1101	90-94	-0.014637804135077204
1101	95-99	0.0045658119964286925
1101	100-104	-0.12105126885638384
1101	105-109	-0.005933459820646192
1101	110-114	-0.12396065791806876
1101	115-119	-0.12619843099055572
1101	120-124	-0.13185085466441393
1101	125-129	-0.12459851197159111
1101	130-134	-0.157129314349028
1101	135-139	-0.10059706710156746
1101	140-144	-0.18848906239654895
1101	145-149	-0.3575229275528926
1101	150-154	-0.11867888104930557
1101	155-159	-0.3676997547634997
1101	160-164	-0.22906228177937749
1101	165-169	-0.33809300074572235
1101	170-174	-0.6101229722560859
1101	175-179	-0.25067877166353014
1101	180-184	-0.6045358834043668
1101	185-189	-0.34174598011946244
1101	190-194	-0.48367554972991655
1101	195-199	-0.25453421443315705
1101	200-204	-0.08981161172702201
1101	205-209	-0.3821191181039083
1101	210-214	-0.269318859842258
1101	215-219	-0.4642821677440523
1101	220-224	-0.41356603461590424
1101	225-229	-0.39159598888169
1101	230-234	-0.41682183234728143
1101	235-239	-0.45908748711079284
1101	240-244	-0.5333883064216209
1101	245-249	-0.35095911659255563
1101	250-254	-0.37559966262957545
1101	255-259	-0.7334791570930292
1101	260-264	-0.4538382598079309
1101	265-269	-0.6324125655328814
1101	270-274	-0.6377576276742758
1101	275-279	-0.7545100933326871
1101	280-284	-0.7760223945808988
1101	285-289	-1.3615174802349834
1101	290-294	-0.5608281309804255
1101	295-299	-0.902192223461924
1101	300-301	-1.1166114115637313
1102	1	0.015078143061927562
1102	2	0.32068639832287715
1102	3	0.19143258261371443
1102	4	-0.044873601815993425
1102	5	0.08284139189014894
1102	6	0.4697138094011777
1102	7	0.0027797485522143006
1102	8	0.18426496617770027
1102	9	0.20653611031882946
1102	10-14	0.11504649771218567
1102	15-19	-0.0870926341671705
1102	20-24	-0.06744513423728193
1102	25-29	-0.014164706819052242
1102	30-34	-0.03498752356512824
1102	35-39	0.02298969314133359
1102	40-44	-0.28184531044878725
1102	45-49	0.0603112065457978
1102	50-54	-0.04010892882572392
1102	55-59	0.011917596181532986
1102	60-64	0.045479241487633715
1102	65-69	-0.19445515518648193
1102	70-74	-0.10847569074935848
1102	75-79	0.09408285248742487
1102	80-84	0.06459953949617159
1102	85-89	-0.09317932391753914
1102	90-94	-0.3321777985775327
1102	95-99	-0.04373478137731723
1102	100-104	0.005823986238034706
1102	105-109	-0.07305937940492413
1102	110-114	-0.17423505774399928
1102	115-119	-0.014886493941162371
1102	120-124	-0.14077817786546376
1102	125-129	-0.08371525007445513
1102	130-134	-0.13435632296452837
1102	135-139	-0.09160043703036536
1102	140-144	-0.09991103003798685
1102	145-149	-0.11013070566563954
1102	150-154	-0.042238322311490606
1102	155-159	-0.1370555983973034
1102	160-164	-0.22301584172309674
1102	165-169	-0.15635912977798228
1102	170-174	-0.24411490773996292
1102	175-179	-0.22767473940546523
1102	180-184	-0.12350765759791926
1102	185-189	0.07737425448464563
1102	190-194	-0.11969536973192874
1102	195-199	-0.06543319309631812
1102	200-204	0.27944109329876454
1102	205-209	-0.10748669824345569
1102	210-214	-0.1183369607624627
1102	215-219	-0.33952941215703447
1102	220-224	0.004304326391277868
1102	225-229	0.03526820365546257
1102	230-234	-0.28418348847790753
1102	235-239	0.008476779848976435
1102	240-244	-0.045374509752399206
1102	245-249	-0.2053003728206093
1102	250-254	-0.38413106833079524
1102	255-259	-0.543149989405368
1102	260-264	-0.426014197078203
1102	265-269	-0.526029326130768
1102	270-274	-0.48344495921066155
1102	275-279	-0.49308723945700805
1102	280-284	-0.6307148719148863
1102	285-289	-0.8069842775885689
1102	290-294	-0.573945830890576
1102	295-299	-1.147278979003442
1102	300-301	-1.2555249611591428
1103	1	-0.9450955542085389
1103	2	-0.09966099621806279
1103	3	-0.05188834377172924
1103	4	-0.2555435770021006
1103	5	-0.21860608536378123
1103	6	-0.20747808472619766
1103	7	-0.055284767576814886
1103	8	-0.2052511628545588
1103	9	-0.09981211135197299
1103	10-14	-0.15815863049294165
1103	15-19	-0.09199337858404277
1103	20-24	0.009370415804077936
1103	25-29	-0.04075693179837003
1103	30-34	-0.029139715459251647
1103	35-39	-0.027266717115075778
1103	40-44	-0.028693945684509004
1103	45-49	0.0399844902513351
1103	50-54	-0.02524126960322093
1103	55-59	-0.22281358661416562
1103	60-64	0.17566121005670254
1103	65-69	-0.027341838395749107
1103	70-74	-0.2248404550173504
1103	75-79	-0.20207512931571614
1103	80-84	-0.05817134553277725
1103	85-89	-0.13918842234598827
1103	90-94	0.009141566633537934
1103	95-99	-0.05311347653341869
1103	100-104	-0.0970844257524135
1103	105-109	-0.07603832349786899
1103	110-114	-0.11600043477957911
1103	115-119	-0.18003533742213307
1103	120-124	0.044478538242820775
1103	125-129	-0.009218828541420976
1103	130-134	-0.14661438748066047
1103	135-139	-0.13869721122392065
1103	140-144	-0.10378199777993302
1103	145-149	-0.08581710299313272
1103	150-154	-0.203480623177974
1103	155-159	-0.29364546014844706
1103	160-164	-0.16643435869000456
1103	165-169	-0.12444040334311524
1103	170-174	-0.1744777990254036
1103	175-179	-0.24184937426939257
1103	180-184	-0.1547170723294542
1103	185-189	-0.36016024346570674
1103	190-194	-0.16927594230998722
1103	195-199	-0.3240794120280981
1103	200-204	0.04569175432467176
1103	205-209	0.013401720582557175
1103	210-214	-0.36190121248113627
1103	215-219	-0.16292444124218974
1103	220-224	-0.2864413108377022
1103	225-229	-0.24362127281200685
1103	230-234	-0.3136078953515522
1103	235-239	-0.48094334853981024
1103	240-244	-0.32406363377496206
1103	245-249	-0.33396973624406456
1103	250-254	-0.5856192522779899
1103	255-259	-0.5041421120368348
1103	260-264	-0.42688496565389755
1103	265-269	-0.4503278145276184
1103	270-274	-0.5643583081567911
1103	275-279	-0.3991208779496631
1103	280-284	-0.5869039287596749
1103	285-289	-0.46540241682637884
1103	290-294	-0.9312292032559171
1103	295-299	-0.7866828310225316
1103	300-301	-0.9510109681312606
1104	1	0.46741928018567336
1104	2	0.051455629092110655
1104	3	-0.266064630318283
1104	4	-0.10005754830428515
1104	5	0.14499300838959073
1104	6	0.10502596101768091
1104	7	0.251943628150876
1104	8	0.19471647119442537
1104	9	0.24234993417613282
1104	10-14	-0.13481414888758536
1104	15-19	0.08021505814052432
1104	20-24	-0.015661410714422175
1104	25-29	0.01967029875508075
1104	30-34	0.03853533039251289
1104	35-39	-0.04278511176390509
1104	40-44	-0.006910965540697589
1104	45-49	-0.264215553048075
1104	50-54	-0.11909275260821062
1104	55-59	-0.009447057062317299
1104	60-64	0.01947170440627133
1104	65-69	-0.09634585707718912
1104	70-74	-0.019075276348935688
1104	75-79	-0.25530202189744244
1104	80-84	-0.13722641232977395
1104	85-89	0.024762919024709618
1104	90-94	-0.21256148539209363
1104	95-99	-0.14307328971903388
1104	100-104	-0.12893193308379125
1104	105-109	-0.14419950520743186
1104	110-114	-0.2377425874894712
1104	115-119	-0.5439975400940114
1104	120-124	-0.16429956537398738
1104	125-129	-0.35591373459116227
1104	130-134	-0.4597413560914987
1104	135-139	-0.34429682843643405
1104	140-144	-0.14712550257504375
1104	145-149	-0.14637743626720834
1104	150-154	-0.1600622698237899
1104	155-159	-0.25103044804191654
1104	160-164	-0.21147948305716824
1104	165-169	-0.1159107973558875
1104	170-174	-0.3102712773408314
1104	175-179	0.0029758893534150843
1104	180-184	-0.2153939342518285
1104	185-189	-0.2845502944491969
1104	190-194	-0.1315051018259794
1104	195-199	-0.20191214334234786
1104	200-204	0.059506702921517274
1104	205-209	-0.17998533137632222
1104	210-214	-0.4710870974491783
1104	215-219	-0.26633094304823146
1104	220-224	-0.540889768742673
1104	225-229	-0.3843381386080651
1104	230-234	-0.3937958160324655
1104	235-239	-0.1592928522757262
1104	240-244	-0.40286590878331907
1104	245-249	-0.5327586288502957
1104	250-254	-0.5137847479844773
1104	255-259	-0.8041889504443382
1104	260-264	-0.6197989837201376
1104	265-269	-0.5999006928592792
1104	270-274	-0.7899075442446737
1104	275-279	-0.8522892758408993
1104	280-284	-0.8750435807487129
1104	285-289	-1.169740804338275
1104	290-294	-1.1315371829206633
1104	295-299	-1.3090125959798051
1104	300-301	-0.9021519105013098
1201	1	-0.4473878475391082
1201	2	-0.04797548641617766
1201	3	0.10070993441518539
1201	4	0.0784259282359443
1201	5	0.06121965885362357
1201	6	-0.08805063944043923
1201	7	0.08155788360044625
1201	8	-0.03237490520493935
1201	9	-0.3770136464624372
1201	10-14	0.03611789683824895
1201	15-19	-0.224904902284905
1201	20-24	-5.434935414285746E-4
1201	25-29	0.030781290377753123
1201	30-34	-0.05458105971814575
1201	35-39	0.030096626936362725
1201	40-44	-0.12191456595212458
1201	45-49	0.06046991707427907
1201	50-54	0.12550035470961518
1201	55-59	0.069205913437699
1201	60-64	-0.06970337867890208
1201	65-69	0.011019327458413386
1201	70-74	0.03899524667334475
1201	75-79	0.10467935673344897
1201	80-84	0.0011606739672771482
1201	85-89	0.0738330484557892
1201	90-94	-0.045051981753552184
1201	95-99	0.0726929750794909
1201	100-104	0.04098009037660688
1201	105-109	-0.1663609041430263
1201	110-114	-0.1727746283279714
1201	115-119	-0.05874501484148453
1201	120-124	-0.031005332724916457
1201	125-129	0.0017489133940671309
1201	130-134	-0.03500148425485605
1201	135-139	0.037431821034147106
1201	140-144	-0.021201352618639646
1201	145-149	-0.019162963730160243
1201	150-154	-0.08223832231149686
1201	155-159	-0.07382979194569117
1201	160-164	-0.02043519656181303
1201	165-169	-0.04603654913281474
1201	170-174	-0.16282458515931353
1201	175-179	-0.1876747394054732
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2404	265-269	0.6362431764531493
2404	270-274	0.6723879621931985
2404	275-279	0.786884000375828
2404	280-284	0.98553012875918
2404	285-289	1.003704168913032
2404	290-294	1.0949617321346246
2404	295-299	1.166767144924954
2404	300-301	1.4801382016421805
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	6.0
17	22.0
18	63.0
19	91.0
20	167.0
21	246.0
22	315.0
23	347.0
24	358.0
25	438.0
26	467.0
27	590.0
28	691.0
29	1077.0
30	1617.0
31	3133.0
32	9041.0
33	72933.0
34	2285.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.357877022377965	0.039409076869002094	0.1342038833917369	99.4685100173613
2	0.07669201763916406	0.14060203233846744	0.3515050808461686	99.4312008691762
3	0.18958961304546953	0.22260802880057942	99.4248404997497	0.16296185840425192
4	0.08307859448059902	99.43016605067794	0.2684077667834738	0.2183475880579846
5	0.22473824917187682	0.2513660038130945	0.1981104945306592	99.32578525248437
6	99.34708745619734	0.2556264445556893	0.07349260280976067	0.32379349643720645
7	99.78484774249895	0.06390661113892232	0.04473462779724562	0.10651101856487054
8	99.47596578866083	0.18106873156027992	0.1001203574509783	0.24284512232790484
9	30.140488033487063	23.281178437909404	25.27932514618637	21.299008382417163
10-14	59.69665661912725	10.165198589794114	9.90595077060722	20.232194020471418
15-19	23.037055183358717	37.01449614962668	19.61592126705508	20.332527399959528
20-24	56.946116075708034	40.05687688391364	2.418226165496821	0.5787808748815065
25-29	2.6990064588694422	3.8608360742343937	58.350943267608514	35.089214199287646
30-34	3.5083346647494276	57.72679341747883	0.8942855621238749	37.870586355647866
35-39	47.102695033647976	14.536693231420116	20.530911616274587	17.829700118657318
40-44	37.43118288733594	47.36002215783194	1.6156042270325548	13.593190727799556
45-49	20.321083518366198	20.012742762409278	33.07599203896984	26.590181680254688
50-54	54.84340471416059	20.429734032011627	19.513198243217612	5.213663010610165
55-59	16.454058799962493	42.33354359704381	2.286617851389019	38.925779751604686
60-64	39.02924582392825	2.0513214013952568	1.1139033295606413	57.80552944511585
65-69	40.37964295230482	21.182414068745004	35.665973887556625	2.7719690913935517
70-74	40.83307575063152	55.54726553756622	3.108472516813933	0.5111861949883288
75-79	46.86863603504859	22.01220175328234	27.74118294313454	3.377979268534532
80-84	38.78324968699277	1.1306653243437512	39.77420820366906	20.311876784994425
85-89	21.875426650283295	10.356722301863607	39.712821694313604	28.05502935353949
90-94	18.33605429111047	56.675736525923796	2.67592859353159	22.312280589434145
95-99	20.299020340213005	1.3836894115638272	58.0272341152114	20.29005613301176
100-104	35.18772722711931	10.753620156313044	47.439151699653515	6.61950091691413
105-109	40.867584295690904	45.357091769307466	12.569895021360816	1.205428913640818
110-114	43.303569521286825	47.41439343772029	1.386580650673958	7.895456390318928
115-119	26.033578062047813	42.686672437562116	24.032787235634213	7.246962264755859
120-124	53.06749843402265	25.69656596666232	16.687867042643756	4.548068556671278
125-129	4.826021600562786	37.14219123190776	36.14598234236315	21.885804825166304
130-134	34.78936153826406	12.248307789729502	22.972128804798945	29.99020186720749
135-139	12.830803345919056	23.06548001267134	49.43064581032372	14.673070831085882
140-144	40.454532792488166	18.801869387177856	19.450069319893633	21.29352850044035
145-149	16.985197174493262	35.65905266204324	36.82484216393513	10.530907999528367
150-154	6.163649075849407	29.728524061372692	21.626710933244116	42.481115929533786
155-159	22.434050622864167	70.16580393132726	1.4576759132731099	5.942469532535455
160-164	22.551222055576655	18.74505159218039	16.54361357682182	42.16011277542114
165-169	37.44433069125452	23.49797497859079	21.08361717973648	17.974077150418204
170-174	3.229879282134529	40.1888283367045	20.85979807349575	35.72149430766522
175-179	42.27241285976361	4.355830329485257	19.08943662274389	34.28232018800724
180-184	37.09583482874895	38.96899063207188	1.1979084956083135	22.73726604357086
185-189	39.07330195312752	2.2887910736288	7.744775281744504	50.89313169149917
190-194	20.643079618663858	37.91319845795208	35.58592009505421	5.8578018283298565
195-199	19.95497872798751	37.517367655770364	22.969034412192364	19.55861920404976
200-204	72.39087682323682	3.2727737917905286	21.70408001751955	2.632269367453095
205-209	21.55923260460515	38.82799988792113	4.316114139395938	35.296653368077784
210-214	36.11406416109062	10.260297279288565	50.02576880529781	3.599869754323014
215-219	38.297188512461226	20.065876039154613	4.21938834938891	37.41754709899525
220-224	7.555762663232131	21.445352697901093	67.82948997577272	3.1693946630940593
225-229	54.17392186181793	3.180202864965984	21.306431393364274	21.33944387985181
230-234	20.687764915097677	54.94911040323207	21.07839328723487	3.2847313944353798
235-239	54.7556442107172	21.213122115289128	3.9504077993514937	20.080825874642176
240-244	37.808859218060434	23.037378334729553	9.998143174222077	29.155619272987938
245-249	51.77930486387962	12.54778142992273	14.621441498252874	21.051472207944784
250-254	18.229147539354557	34.16582596504266	33.18506741094864	14.419959084654153
255-259	22.46605415777146	34.86741458439572	19.034520609512615	23.632010648320207
260-264	38.14429425048894	20.5523679859963	35.92250424108831	5.3808335224264425
265-269	22.538666366960896	54.30841452766082	18.020818023843898	5.132101081534379
270-274	19.83873757025508	4.77431906614786	31.100734976221357	44.2862083873757
275-279	37.265475837821825	7.519044107948686	19.8908490980131	35.32463095621639
280-284	4.76917520712273	38.0197531611762	16.584524023208992	40.62654760849209
285-289	47.588620573297455	41.03844190453118	6.2516763715964245	5.1212611505749415
290-294	31.85245444101401	20.076892620774856	22.12858852062838	25.94206441758275
295-299	34.744994572493795	26.36479394041319	19.530941153601702	19.359270333491317
300-301	8.390084600636069	90.54430012459676	0.8778667212844278	0.18774855348273567
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	0.5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	0.5
22	0.5
23	1.5
24	1.0
25	0.25
26	0.25
27	0.5833333333333333
28	0.3333333333333333
29	1.3333333333333333
30	0.5
31	0.75
32	1.0
33	1.5
34	1.5
35	2.5
36	1.0
37	2.833333333333333
38	5.166666666666666
39	6.249999999999999
40	14.500000000000009
41	9.666666666666668
42	7.75
43	16.333333333333336
44	28.83333333333331
45	511.9999999999889
46	1156.416666666682
47	311.9999999999997
48	190.58333333333346
49	296.16666666666674
50	5006.666666666665
51	4605.666666666668
52	7897.416666666637
53	2306.2499999999995
54	2988.9166666666697
55	5647.999999999994
56	979.666666666668
57	226.33333333333337
58	142.08333333333334
59	91.58333333333331
60	51.916666666666664
61	29.166666666666664
62	19.083333333333332
63	19.333333333333332
64	16.66666666666667
65	12.000000000000002
66	8.583333333333332
67	11.0
68	13.083333333333332
69	14.583333333333332
70	18.333333333333336
71	15.0
72	9.25
73	3.75
74	1.0
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005325550928243526
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.6321463890738215
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-29	2.0
30-39	3.0
40-49	27.0
50-59	6.0
60-69	28.0
70-79	52.0
80-89	49.0
90-99	33.0
100-109	20.0
110-119	107.0
120-129	50.0
130-139	107.0
140-149	147.0
150-159	33.0
160-169	52.0
170-179	78.0
180-189	103.0
190-199	161.0
200-209	50.0
210-219	72.0
220-229	21.0
230-239	49.0
240-249	42.0
250-259	44.0
260-269	23.0
270-279	40.0
280-289	34.0
290-299	4225.0
300-302	88229.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.921224450669421
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.13526959795617	4.921874167882667
2	13.6345300524405	2.160043456495574
3	6.3735377168213	1.514586683992459
4	4.0876697593115505	1.2951739857488258
5	2.5413473174667205	1.0065291254380266
6	2.0034960333467797	0.9522085059699426
7	1.3311819281968535	0.7381213586545529
8	1.210165389269867	0.7668793336670678
9	0.9009009009009009	0.6422614419461693
>10	4.746537582358478	5.85810602106788
>50	0.28237192416296897	1.572102634017489
>100	0.41683474519295416	9.010832170588047
>500	0.01344628210299852	0.5389457539382448
>1k	0.3227107704719645	69.02233536059306
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4264	4.5416298316060795	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3724	3.9664703313557785	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3534	3.764099396082525	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3471	3.6969974543866564	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3382	3.6022026478639217	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3252	3.46373832372959	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3103	3.3050369060679325	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3071	3.270953380127174	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2951	3.1431401578493294	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2715	2.8917741540362347	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	2710	2.8864486031079917	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	2681	2.855560407724179	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2631	2.802304898441744	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2515	2.678752116906494	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2402	2.55839466592819	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	2381	2.5360273520295675	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2247	2.393302587152641	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	2082	2.2175594065206043	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2078	2.2132989657780096	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	2070	2.2047780842928204	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	2065	2.1994525333645765	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1961	2.0886810740571113	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1831	1.9502167499227796	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1682	1.7915153322611226	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	506	0.5389457539382448	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	451	0.4803646937275662	No Hit
CCTACGGGGGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	449	0.47823447335626873	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	436	0.46438804094283553	No Hit
CCTACGGGGGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	435	0.46332293075718683	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	430	0.4579973798289433	No Hit
CCTACGGGTGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	407	0.4334998455590231	No Hit
CCTACGGGTGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	403	0.4292394048164282	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	399	0.4249789640738334	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	383	0.40793720110345416	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	375	0.3994163196182645	No Hit
CCTACGGGAGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	365	0.3887652177617774	No Hit
CCTACGGGAGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	357	0.3802443362765878	No Hit
CCTACGGGCGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	332	0.3536165816353702	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	320	0.3408352594075857	No Hit
CCTACGGGCGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	271	0.28864486031079917	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	228	0.24284512232790484	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	220	0.23432424084271516	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	194	0.20663137601584886	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	191	0.20343604545890273	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	181	0.19278494360241566	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	169	0.1800036213746312	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	167	0.1778734010033338	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	167	0.1778734010033338	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	166	0.1768082908176851	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	153	0.16296185840425192	No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	150	0.1597665278473058	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	144	0.15337586673341358	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	139	0.14805031580517003	No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	136	0.14485498524822393	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	131	0.1395294343199804	No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	111	0.1182272306070063	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	94	0.1001203574509783	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	8.520881485189642E-4	0.0
120-124	0.0	0.0	0.0	0.0010651101856487054	0.0
125-129	0.0	0.0	0.0	0.0010651101856487054	0.0
130-134	0.0	0.0	0.0	0.0010651101856487054	0.0
135-139	0.0	0.0	0.0	0.0010651101856487054	0.0
140-144	0.0	0.0	0.0	0.0010651101856487054	0.0
145-149	0.0	0.0	0.0	0.0010651101856487054	0.0
150-154	0.0	0.0	0.0	0.0010651101856487054	0.0
155-159	0.0	0.0019171983341676698	0.0	0.0010651101856487054	0.0
160-164	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
165-169	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
170-174	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
175-179	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
180-184	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
185-189	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
190-194	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
195-199	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
200-204	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
205-209	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
210-214	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
215-219	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
220-224	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
225-229	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
230-234	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
235-239	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
240-244	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
245-249	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
250-254	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
255-259	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
260-264	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
265-269	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
270-274	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
275-279	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
280-284	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
285-289	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
290	0.0	0.0042604407425948215	0.0	0.0010651101856487054	0.0
>>END_MODULE
