##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	DCFS2_cleaned_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	71052
Sequences flagged as poor quality	0
Sequence length	36-301
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.229001294826325	34.0	34.0	34.0	20.0	34.0
2	33.097956426279346	34.0	34.0	34.0	34.0	34.0
3	33.57056803467883	34.0	34.0	34.0	34.0	34.0
4	33.43828463660418	34.0	34.0	34.0	34.0	34.0
5	33.243539942577264	34.0	34.0	34.0	34.0	34.0
6	33.05186342397118	34.0	34.0	34.0	34.0	34.0
7	33.49857850588302	34.0	34.0	34.0	34.0	34.0
8	33.47031751393346	34.0	34.0	34.0	34.0	34.0
9	33.39890502730395	34.0	34.0	34.0	34.0	34.0
10-14	33.327239205089235	34.0	34.0	34.0	34.0	34.0
15-19	33.456291167032596	34.0	34.0	34.0	34.0	34.0
20-24	33.68030456567021	34.0	34.0	34.0	34.0	34.0
25-29	33.63587794854473	34.0	34.0	34.0	34.0	34.0
30-34	33.54262230479086	34.0	34.0	34.0	34.0	34.0
35-39	33.58180095613403	34.0	34.0	34.0	34.0	34.0
40-44	33.35945699403787	34.0	34.0	34.0	34.0	34.0
45-49	33.59998772744737	34.0	34.0	34.0	34.0	34.0
50-54	33.5599712854006	34.0	34.0	34.0	34.0	34.0
55-59	33.355019178576214	34.0	34.0	34.0	34.0	34.0
60-64	33.304103375693515	34.0	34.0	34.0	31.2	34.0
65-69	33.52817990946723	34.0	34.0	34.0	34.0	34.0
70-74	33.565633660174164	34.0	34.0	34.0	34.0	34.0
75-79	33.382722177281764	34.0	34.0	34.0	34.0	34.0
80-84	33.601534907398914	34.0	34.0	34.0	34.0	34.0
85-89	33.56207378859368	34.0	34.0	34.0	34.0	34.0
90-94	33.06050827943686	34.0	34.0	34.0	34.0	34.0
95-99	33.307808550611306	34.0	34.0	34.0	34.0	34.0
100-104	33.3000446520161	34.0	34.0	34.0	34.0	34.0
105-109	33.371198895887495	34.0	34.0	34.0	34.0	34.0
110-114	33.2312703594028	34.0	34.0	34.0	34.0	34.0
115-119	33.094188941374284	34.0	34.0	34.0	34.0	34.0
120-124	33.25696418918812	34.0	34.0	34.0	34.0	34.0
125-129	33.31892740875965	34.0	34.0	34.0	34.0	34.0
130-134	33.04618834966279	34.0	34.0	34.0	34.0	34.0
135-139	33.154329793647015	34.0	34.0	34.0	34.0	34.0
140-144	33.271956916672174	34.0	34.0	34.0	34.0	34.0
145-149	33.37282289374563	34.0	34.0	34.0	34.0	34.0
150-154	33.02985697185824	34.0	34.0	34.0	34.0	34.0
155-159	33.16602160142734	34.0	34.0	34.0	34.0	34.0
160-164	33.27688355051701	34.0	34.0	34.0	34.0	34.0
165-169	33.30867621108813	34.0	34.0	34.0	34.0	34.0
170-174	32.89718189551302	34.0	34.0	34.0	31.2	34.0
175-179	33.25118168488673	34.0	34.0	34.0	34.0	34.0
180-184	33.00891337520942	34.0	34.0	34.0	34.0	34.0
185-189	33.208097169152374	34.0	34.0	34.0	34.0	34.0
190-194	33.14328157248256	34.0	34.0	34.0	34.0	34.0
195-199	32.593180116855976	34.0	34.0	34.0	28.4	34.0
200-204	32.969092225259146	34.0	34.0	34.0	34.0	34.0
205-209	32.700134573487716	34.0	34.0	34.0	31.2	34.0
210-214	32.73497027610465	34.0	34.0	34.0	31.2	34.0
215-219	32.94838078570014	34.0	34.0	34.0	34.0	34.0
220-224	32.93508891192936	34.0	34.0	34.0	34.0	34.0
225-229	33.068453027951605	34.0	34.0	34.0	34.0	34.0
230-234	32.777670769681706	34.0	34.0	34.0	31.2	34.0
235-239	32.83046540774952	34.0	34.0	34.0	34.0	34.0
240-244	32.621907998189	34.0	34.0	34.0	31.2	34.0
245-249	32.882121598025655	34.0	34.0	34.0	34.0	34.0
250-254	32.36746116584801	34.0	34.0	34.0	31.2	34.0
255-259	32.3558028081702	34.0	34.0	34.0	31.2	34.0
260-264	32.6083540369646	34.0	34.0	34.0	31.2	34.0
265-269	32.76874004435602	34.0	34.0	34.0	34.0	34.0
270-274	32.42785788208672	34.0	34.0	34.0	34.0	34.0
275-279	32.54660921829493	34.0	34.0	34.0	31.2	34.0
280-284	32.01713957424806	34.0	34.0	34.0	22.8	34.0
285-289	31.613424485604696	34.0	34.0	34.0	20.0	34.0
290-294	31.821838505841914	34.0	34.0	34.0	20.0	34.0
295-299	31.877236596997836	34.0	34.0	34.0	20.0	34.0
300-301	31.66504033435821	34.0	34.0	34.0	20.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.22070479453992675
1101	2	-0.42313601009649204
1101	3	-0.13893133302752148
1101	4	-0.14137127596666232
1101	5	-0.34314525126084305
1101	6	-0.5270678106919391
1101	7	-0.3685289261045881
1101	8	-0.21272385231138458
1101	9	-0.3451815170658108
1101	10-14	-0.09338606519565218
1101	15-19	0.06426385140390778
1101	20-24	-0.09152831315591925
1101	25-29	0.09867143683021595
1101	30-34	-0.13783347294786097
1101	35-39	-0.09678584945424973
1101	40-44	-0.3291791095615082
1101	45-49	-0.08806801442047174
1101	50-54	0.09696711608111741
1101	55-59	-0.027586517289940105
1101	60-64	-0.14315949810711714
1101	65-69	0.08679265232571254
1101	70-74	-0.24858904362391598
1101	75-79	-0.054136085187629135
1101	80-84	0.06822332327534042
1101	85-89	-0.05937335686483891
1101	90-94	-0.3442342196217538
1101	95-99	-0.2295621958972447
1101	100-104	-0.05687255805056424
1101	105-109	-0.05574728907777171
1101	110-114	-0.11995128786443843
1101	115-119	-0.19994275919493987
1101	120-124	-0.0916754690078676
1101	125-129	-0.11141997828534045
1101	130-134	-0.06979528263543955
1101	135-139	-0.012470778682043715
1101	140-144	0.05590602574504544
1101	145-149	0.044462322109744434
1101	150-154	0.04528933461243412
1101	155-159	0.14095874718873347
1101	160-164	0.012344964573614448
1101	165-169	-0.03080794354715266
1101	170-174	-0.2291473734222791
1101	175-179	-0.03622179466346154
1101	180-184	-0.2113680369901516
1101	185-189	0.010145225948754444
1101	190-194	0.0030781424189427753
1101	195-199	-0.24277276402207804
1101	200-204	0.2606233718331836
1101	205-209	-0.10750800126382387
1101	210-214	-0.07708939257254599
1101	215-219	-0.036413121855680686
1101	220-224	-0.278285138392512
1101	225-229	0.041826054944785085
1101	230-234	-0.3281499461051709
1101	235-239	-0.30630015264834043
1101	240-244	-0.3070521999227154
1101	245-249	-0.3735624499808168
1101	250-254	-0.0417930149892527
1101	255-259	-0.4761185598832256
1101	260-264	-0.5299133922677512
1101	265-269	-0.4017569551699154
1101	270-274	-0.3739573371691307
1101	275-279	-0.4154935472004837
1101	280-284	-0.6190643890818173
1101	285-289	-0.5635591441068399
1101	290-294	-0.44961881705246043
1101	295-299	-0.6288272850669294
1101	300-301	-0.4537269109002935
1102	1	-0.5874953698286269
1102	2	0.06060063495875312
1102	3	-0.08733787124320713
1102	4	-0.2079040127030325
1102	5	0.03594341644073751
1102	6	-0.31384527393730366
1102	7	0.07689158343792002
1102	8	0.06644699966560097
1102	9	-0.20552303838806552
1102	10-14	0.14189838371312646
1102	15-19	-0.040530231328261834
1102	20-24	0.06863240276042859
1102	25-29	0.08409742532442266
1102	30-34	-0.05889638957648202
1102	35-39	0.08618191604661263
1102	40-44	0.12327272132151279
1102	45-49	-0.04125585114771724
1102	50-54	0.05046177410321917
1102	55-59	-0.05525522334418298
1102	60-64	0.044520165850514104
1102	65-69	-0.029280765626978678
1102	70-74	-0.045414904275915546
1102	75-79	0.13870109863119495
1102	80-84	-0.004825713342327731
1102	85-89	0.06103016477860024
1102	90-94	-0.13329511470612943
1102	95-99	-0.07050863757002901
1102	100-104	0.09751482272712764
1102	105-109	-0.03096300227954174
1102	110-114	0.04052616571646439
1102	115-119	-0.15721508457070854
1102	120-124	0.08378872197356912
1102	125-129	-0.13188859013325782
1102	130-134	-0.20267916120897667
1102	135-139	-0.14357157443801327
1102	140-144	-0.06804770251902426
1102	145-149	-0.05737233748353532
1102	150-154	-0.1015276415148918
1102	155-159	0.06831654264997411
1102	160-164	-0.24735909035391046
1102	165-169	0.01683894116499829
1102	170-174	0.14219715817458933
1102	175-179	0.07105584334156845
1102	180-184	0.08648604026306828
1102	185-189	-0.024296834137082612
1102	190-194	-0.305682945377832
1102	195-199	-0.41431969441953953
1102	200-204	0.21228599545699467
1102	205-209	-0.24021186392477034
1102	210-214	-0.25629073610679143
1102	215-219	0.022806881876356044
1102	220-224	-0.1226441605981492
1102	225-229	0.13189136563708104
1102	230-234	0.0479687327528282
1102	235-239	-0.12163510319881254
1102	240-244	0.10745423784552344
1102	245-249	-0.06256039735905716
1102	250-254	-0.5901975680775102
1102	255-259	-0.6941069905605914
1102	260-264	-0.18485647866447152
1102	265-269	-0.12443655957371647
1102	270-274	-0.3000853461260249
1102	275-279	-0.44953348002376714
1102	280-284	-0.7000197912090798
1102	285-289	-0.7301424618900114
1102	290-294	-0.6550952836095583
1102	295-299	-0.8372503711228454
1102	300-301	-0.26840904243706376
1103	1	-0.13688231537496165
1103	2	-0.016283605935583978
1103	3	-0.29703855642171106
1103	4	0.07916775073007187
1103	5	-0.12649798276589763
1103	6	-0.3293519454122418
1103	7	-0.3706021057074125
1103	8	-0.42709789755762273
1103	9	-0.4268356961594364
1103	10-14	-0.4245819624823284
1103	15-19	-0.27107476793122487
1103	20-24	-0.07433910823849033
1103	25-29	-0.07224949137229686
1103	30-34	-0.024590583590175186
1103	35-39	-0.019259843780289998
1103	40-44	-0.13473304860274737
1103	45-49	-0.018147304447396095
1103	50-54	-0.004698701915536674
1103	55-59	-0.22018490239214117
1103	60-64	-0.08232440681447173
1103	65-69	-0.10691870287905658
1103	70-74	-0.00698360243674756
1103	75-79	-0.23729241020399883
1103	80-84	-0.1802927165879069
1103	85-89	-0.20855512191451453
1103	90-94	-0.12547326111795343
1103	95-99	-0.09098826252495229
1103	100-104	-0.36940908639656556
1103	105-109	-0.4385497437826231
1103	110-114	-0.5017163375429803
1103	115-119	-0.49063533663503023
1103	120-124	-0.40389054790909285
1103	125-129	-0.5003153611024089
1103	130-134	-0.3002599231965206
1103	135-139	-0.3080435431472779
1103	140-144	-0.2754937404735429
1103	145-149	-0.38290471471082554
1103	150-154	-0.3238222960129349
1103	155-159	-0.27936562452371305
1103	160-164	-0.3740110919815933
1103	165-169	-0.5299348694458956
1103	170-174	-0.4318289581618231
1103	175-179	-0.504361926725096
1103	180-184	-0.44565790787576987
1103	185-189	-0.4148547740512498
1103	190-194	-0.42717151879773496
1103	195-199	-0.5021484607890727
1103	200-204	-0.20810944124745134
1103	205-209	-0.28596953972535744
1103	210-214	-0.6095903215952276
1103	215-219	-0.4233176183253988
1103	220-224	-0.4217931279874634
1103	225-229	-0.31181943000505186
1103	230-234	-0.4718214436897128
1103	235-239	-0.6202264974983578
1103	240-244	-0.31486335872099147
1103	245-249	-0.31921275787408376
1103	250-254	-0.3532876968328722
1103	255-259	-0.6082700806893442
1103	260-264	-0.44665905377176784
1103	265-269	-0.5484708943376688
1103	270-274	-0.40308963797151875
1103	275-279	-0.4392330508392206
1103	280-284	-1.0857975032114489
1103	285-289	-0.6702083195191335
1103	290-294	-0.9219975404962746
1103	295-299	-0.5705337376844852
1103	300-301	-1.2790707000389503
1104	1	0.3970159897097787
1104	2	-0.147639538138975
1104	3	0.08008008764608121
1104	4	0.18509995411989877
1104	5	-0.15192171157945467
1104	6	-0.4734197420224078
1104	7	0.12516060615699587
1104	8	-0.14319959247287528
1104	9	0.009604981806667467
1104	10-14	-0.100853148923008
1104	15-19	-0.10582053064308639
1104	20-24	-0.16925436247576897
1104	25-29	-0.2586901693384007
1104	30-34	-0.19408210901391243
1104	35-39	-0.32688696242436066
1104	40-44	-0.21100423504342558
1104	45-49	-0.2927235756338291
1104	50-54	-0.3385970070002813
1104	55-59	-0.1769645634090935
1104	60-64	-0.2230023729161701
1104	65-69	-0.3442068384722816
1104	70-74	-0.22817004311471578
1104	75-79	-0.35169918986501614
1104	80-84	-0.28791983523197473
1104	85-89	-0.2975381727619748
1104	90-94	-0.2831003797620184
1104	95-99	-0.6842086015080042
1104	100-104	-0.4482226457185945
1104	105-109	-0.44109211666398096
1104	110-114	-0.5739485769948445
1104	115-119	-0.6582516366710962
1104	120-124	-0.30236512418028383
1104	125-129	-0.44411990491776976
1104	130-134	-0.5201877991431374
1104	135-139	-0.45509366936436635
1104	140-144	-0.5777440109387371
1104	145-149	-0.3665230921662044
1104	150-154	-0.43058747057472146
1104	155-159	-0.3684355941021593
1104	160-164	-0.25083658567690037
1104	165-169	-0.305152181684484
1104	170-174	-0.48273956461621736
1104	175-179	-0.2954677618086521
1104	180-184	-0.48947962497298647
1104	185-189	-0.34103503156197235
1104	190-194	-0.10739968357525953
1104	195-199	-0.553375488410957
1104	200-204	-0.10616894016532541
1104	205-209	-0.37154233602777254
1104	210-214	-0.3738873026304006
1104	215-219	-0.24500427692192517
1104	220-224	-0.4814424438599545
1104	225-229	-0.3530871751354496
1104	230-234	-0.5511728981484652
1104	235-239	-0.3353690087782084
1104	240-244	-0.34833975357078373
1104	245-249	-0.373290011092962
1104	250-254	-0.5937420404765064
1104	255-259	-0.7418981700781231
1104	260-264	-0.5784816081598123
1104	265-269	-0.5180912880034967
1104	270-274	-0.4658763776488897
1104	275-279	-0.6448938470505539
1104	280-284	-0.8278649887819647
1104	285-289	-0.7090014229674111
1104	290-294	-0.6852121994005884
1104	295-299	-0.8974436534920365
1104	300-301	-1.4565167614271388
1201	1	-0.9151549442967095
1201	2	0.10740914559704606
1201	3	0.2573429798206206
1201	4	0.1835853489990953
1201	5	-0.08320551972947499
1201	6	0.3755164281903589
1201	7	-0.042257352732292475
1201	8	0.30474487200602596
1201	9	-0.39701240009019756
1201	10-14	0.051685617755687474
1201	15-19	-0.15882810366869649
1201	20-24	0.0873558070157543
1201	25-29	0.05175699979250936
1201	30-34	0.035571695529903025
1201	35-39	0.05724574583384623
1201	40-44	-0.1405570659125175
1201	45-49	0.015765425448030612
1201	50-54	-0.03719780036487208
1201	55-59	0.004319244740933925
1201	60-64	-0.10016068521331789
1201	65-69	-0.005450978392943284
1201	70-74	0.003946797851746453
1201	75-79	-0.21959677370923458
1201	80-84	0.007089180274697071
1201	85-89	-0.14492728202991856
1201	90-94	-0.3341461785359172
1201	95-99	-0.27731714820832565
1201	100-104	-0.07950645386861765
1201	105-109	0.003588734399855298
1201	110-114	-0.12238709123934655
1201	115-119	-0.4539963175221544
1201	120-124	-0.20959916673346868
1201	125-129	0.021412009975847468
1201	130-134	-0.37976226722825146
1201	135-139	-0.39551920127925655
1201	140-144	0.13209181724458574
1201	145-149	-0.21157216064887052
1201	150-154	-0.3486761826289495
1201	155-159	-0.4374895286733391
1201	160-164	-0.13041888077643904
1201	165-169	-0.12215739868970843
1201	170-174	-0.3889883944701964
1201	175-179	-0.31845421741312663
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2302	4	0.0852799451203552
2302	5	-0.0524391857057509
2302	6	0.11507583319882997
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2401	70-74	-0.03058262518830901
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2402	9	0.04067226032046989
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2402	70-74	0.029612504439128884
2402	75-79	0.27060855937633477
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2402	90-94	0.09073250754098439
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2404	1	-0.25198883069140265
2404	2	-0.05655728032526497
2404	3	0.0012820780052820169
2404	4	-0.17456417276549985
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2404	6	0.23300227632621784
2404	7	0.03260112862628972
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2404	9	0.32157046151433377
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2404	15-19	0.18490408450229978
2404	20-24	0.09432828407997107
2404	25-29	0.12017003902732881
2404	30-34	0.2143380433729618
2404	35-39	0.13311925744814346
2404	40-44	0.2164798265333161
2404	45-49	0.17658135361511995
2404	50-54	0.11240594743915722
2404	55-59	0.13785428280456102
2404	60-64	0.13305422792351607
2404	65-69	0.09495503370934699
2404	70-74	0.2959319627941568
2404	75-79	0.29964585756150086
2404	80-84	0.049181704186473496
2404	85-89	0.20312268869037098
2404	90-94	0.39247884586384885
2404	95-99	0.20730904738019262
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2404	105-109	0.351568433794732
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2404	115-119	0.44781902505054916
2404	120-124	0.2890110226087188
2404	125-129	0.36347720614996604
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2404	135-139	0.45148841144765584
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2404	145-149	0.29953192033816123
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2404	175-179	0.4163485944539076
2404	180-184	0.5023934308997582
2404	185-189	0.3906498131047158
2404	190-194	0.5384679385848443
2404	195-199	0.8603544796955163
2404	200-204	0.5149831644139624
2404	205-209	0.6965286959838863
2404	210-214	0.5725236907940214
2404	215-219	0.4379307154000003
2404	220-224	0.5691580207618543
2404	225-229	0.49072114868231864
2404	230-234	0.6299114818964284
2404	235-239	0.49779238823718686
2404	240-244	0.7942322037470078
2404	245-249	0.4854174018258348
2404	250-254	0.7851154201940744
2404	255-259	0.6948291459873417
2404	260-264	0.46704914029085387
2404	265-269	0.7140595144754514
2404	270-274	0.7090560869943658
2404	275-279	0.6759149881631714
2404	280-284	0.8568402392491805
2404	285-289	1.1126019458550935
2404	290-294	0.8841029126286521
2404	295-299	0.9331190767830186
2404	300-301	1.1123826049265872
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	4.0
17	17.0
18	36.0
19	81.0
20	112.0
21	174.0
22	218.0
23	265.0
24	296.0
25	307.0
26	386.0
27	417.0
28	599.0
29	815.0
30	1229.0
31	2207.0
32	6827.0
33	55238.0
34	1824.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.46444857287620334	0.056296796712267075	0.11400101334234083	99.3652536170692
2	0.07741027445460943	0.13370865587614356	0.3940886699507389	99.39479239971851
3	0.28007656364352873	0.1885942689860947	99.33288295895963	0.19844620841074143
4	0.09570455441085403	99.35117941789112	0.3434104599448291	0.20970556775319485
5	0.17170522997241455	0.31666948150650226	0.21392782750661488	99.29769746101447
6	99.21747452569949	0.31666948150650226	0.10133423408208073	0.36452175871192927
7	99.73962731520577	0.0605190564656871	0.052074536958847034	0.14777909136970108
8	99.39762427517874	0.1942239486573214	0.11681585317795418	0.2913359229859821
9	30.017452006980804	23.046501154084336	25.543264088273375	21.39278275066149
10-14	59.668974835331866	10.1179417891122	9.950177334909643	20.262906040646286
15-19	22.186849068288016	37.92771491302145	19.610426166751115	20.275009851939423
20-24	56.556324945110624	40.135956764060126	2.6417271857231324	0.6659911051061195
25-29	1.7750380003377808	2.8339807464955244	58.18780611383212	37.20317513933457
30-34	3.0090637842706753	57.94544840398581	0.8934301638236785	38.15205764791983
35-39	43.65065290761336	16.968560788387	20.659972921012113	18.72081338298753
40-44	38.14124217448093	46.843894969148316	1.7922690627392694	13.222593793631491
45-49	20.375192480386005	20.027812887951786	35.3572745621863	24.23972006947592
50-54	56.01711615336974	20.36709644727211	19.940037160069814	3.6757502392883286
55-59	17.198782715917314	41.42313008667941	2.4680688142740914	38.910018383129184
60-64	38.98563741873358	2.189441911932402	1.1898895987430982	57.63503107059091
65-69	40.28168855450184	20.789313630527335	36.352859685085484	2.5761381298853423
70-74	40.9139034060191	55.366958010452336	3.1326027212110286	0.5865358623175346
75-79	45.89657522288366	21.612252828637242	29.61325531231872	2.877916636160375
80-84	38.791969744330586	1.3933828395385053	39.677899900593346	20.13674751553757
85-89	21.78297018994615	11.761508303102678	38.630551362069355	27.824970144881817
90-94	18.981026298367446	56.23343146507403	2.2917752454467344	22.493766991111798
95-99	20.877204351366352	1.0140227598662244	57.81113086500452	20.2976420237629
100-104	36.42686250031703	9.444878106515546	48.725831950154856	5.402427443012576
105-109	39.813311688311686	44.11835993867244	15.085001803751805	0.9833265692640693
110-114	46.12571409236252	46.32901541255223	1.1490331203509978	6.396237374734243
115-119	23.45518455410239	48.87439488145883	22.739198140353647	4.931222424085129
120-124	54.6819713207036	24.100597626424232	18.075800926631338	3.141630126240823
125-129	3.504335260115607	37.66257225433526	36.87059700144509	21.962495484104046
130-134	36.73400226491611	7.3558940064445615	22.51021612721938	33.39988760141995
135-139	8.577661233808803	23.456891290096525	59.071026516264666	8.894420959830008
140-144	40.616721385712665	18.69551681195517	19.66517604437903	21.02258575795313
145-149	17.849374660141383	37.01230288200109	37.77160367953598	7.366718778321552
150-154	4.497526870145101	31.045570179663112	21.394131345008695	43.062771605183094
155-159	21.60648965378502	72.00500947777327	1.604562931564527	4.78393793687718
160-164	21.711020472949798	19.536743885467125	17.60565975164892	41.146575889934155
165-169	37.87528279099831	22.793946713387424	20.596823668826936	18.733946826787324
170-174	2.4676897421135178	40.22070522437797	20.74294449470642	36.56866053880209
175-179	40.60887205458853	4.103502574796782	19.188832616929822	36.09879275368487
180-184	37.424178344455264	39.245317294291624	1.1384956870002583	22.192008674252854
185-189	39.67103151951972	1.5812297128057455	5.408782526957298	53.33895624071723
190-194	19.638627866312213	39.25991214477631	37.142857142857146	3.9586028460543337
195-199	20.913967020928478	37.94328965527053	21.23749768805042	19.905245635750564
200-204	73.30361437504294	3.263931835360407	21.108763370668132	2.323690418928514
205-209	21.248213706210894	38.309525571756346	3.0712866439311557	37.370974078101604
210-214	35.84419801686886	7.836529833846539	52.49458071389302	3.8246914353915704
215-219	37.969456642852464	20.184035268201907	3.5581671705931135	38.28834091835252
220-224	4.779264013863514	21.70705916220368	71.28650787090743	2.2271689530253753
225-229	55.19989737888571	2.0615441627114777	21.153917418508858	21.58464103989396
230-234	20.4809074112092	55.78736969365177	21.142133716553754	2.589589178585278
235-239	54.82507550543725	20.739967544782782	3.134580777271081	21.30037617250889
240-244	38.42616759515191	21.474394630474283	9.064925507243897	31.03451226712991
245-249	53.38301672534709	13.865318443532695	12.52689123103348	20.22477360008674
250-254	16.09643477839007	41.33836358810889	30.446359010653147	12.118842622847897
255-259	21.75422170969252	36.44457257686994	19.515773609033715	22.285432104403824
260-264	38.39675456852719	20.607404996616964	37.066378894417845	3.9294615404379964
265-269	22.585609740079892	56.10996422279519	17.90916073473187	3.395265302393047
270-274	19.729713520321464	4.024309789888247	28.324389470708017	47.92158721908228
275-279	39.075609846677736	4.701940025196617	19.955320285561655	36.267129842564
280-284	3.423082088251514	38.72197315823611	15.382439417447433	42.47250533606494
285-289	50.85298085938393	41.13260079123693	3.9827688719156624	4.031649477463469
290-294	27.762843317814205	19.770015671650327	23.80203388279436	28.66510712774111
295-299	33.957975305076175	26.26557874214745	19.898043180012998	19.878402772763376
300-301	5.2081369125289445	93.6793356514131	0.9134038814008041	0.19912355465715298
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.3333333333333333
23	0.8333333333333333
24	0.5
25	0.0
26	0.6666666666666666
27	0.6666666666666666
28	0.3333333333333333
29	0.5
30	1.0
31	1.0
32	0.5
33	0.0
34	0.0
35	0.0
36	0.6666666666666666
37	4.416666666666666
38	5.583333333333333
39	3.1666666666666665
40	13.58333333333334
41	10.25
42	4.666666666666667
43	10.833333333333334
44	28.99999999999998
45	391.91666666666197
46	915.0000000000322
47	253.75000000000188
48	103.3333333333333
49	175.66666666666669
50	3953.500000000009
51	3782.750000000017
52	7703.916666666645
53	1856.1666666666638
54	1357.6666666666665
55	2831.7500000000014
56	622.4166666666666
57	138.83333333333331
58	58.66666666666668
59	46.75000000000001
60	28.91666666666666
61	18.000000000000007
62	10.333333333333334
63	10.833333333333334
64	11.750000000000002
65	10.833333333333334
66	6.499999999999999
67	11.833333333333334
68	14.583333333333332
69	10.583333333333334
70	15.0
71	14.25
72	5.5
73	2.5
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.5
80	0.5
81	0.0
82	0.0
83	0.25
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0028148398356133537
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.6027490822163408
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-39	1.0
40-49	7.0
50-59	3.0
60-69	13.0
70-79	5.0
80-89	26.0
90-99	23.0
100-109	23.0
110-119	65.0
120-129	49.0
130-139	76.0
140-149	160.0
150-159	25.0
160-169	34.0
170-179	62.0
180-189	70.0
190-199	129.0
200-209	33.0
210-219	75.0
220-229	26.0
230-239	26.0
240-249	31.0
250-259	32.0
260-269	20.0
270-279	38.0
280-289	49.0
290-299	3391.0
300-302	66560.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.17429488262118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.431129476584026	5.10330462196701
2	13.894628099173554	2.2715757473399765
3	7.007575757575757	1.7184597196419522
4	4.356060606060606	1.424308956820357
5	2.7203856749311295	1.1118617350672746
6	1.8078512396694213	0.8866745482182063
7	1.2568870523415978	0.7191915779992119
8	1.050275482093664	0.6868209198896582
9	0.7920110192837466	0.5826718459719642
>10	3.71900826446281	4.776783201035862
>50	0.15495867768595042	1.031638799752294
>100	0.3960055096418733	6.581095535664021
>500	0.06887052341597796	5.047007825254743
>1k	0.34435261707988984	68.05860496537747
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4287	6.033609187637223	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3792	5.336936328322919	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3564	5.016044587062996	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3402	4.788042560378314	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3365	4.735968023419467	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3177	4.471373078871812	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3084	4.340483026515791	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	2672	3.7606260203794406	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2647	3.7254405224342735	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2325	3.2722513089005236	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2206	3.104768338681529	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2062	2.9020998705173677	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1970	2.7726172380791536	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1889	2.6586162247368126	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1764	2.482688735010978	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1666	2.344761583065923	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1279	1.8000900748747397	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1125	1.5833474075325114	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1068	1.5031244722175308	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1013	1.4257163767381635	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	993	1.39756797838203	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	930	1.3089005235602094	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	867	1.2202330687383889	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	796	1.120306254574115	No Hit
CCTACGGGGGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	356	0.5010414907391769	No Hit
CCTACGGGTGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	346	0.4869672915611102	No Hit
CCTACGGGGGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	340	0.47852277205427013	No Hit
CCTACGGGTGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	316	0.44474469402690986	No Hit
CCTACGGGAGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	308	0.4334853346844565	No Hit
CCTACGGGCGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	285	0.40111467657490285	No Hit
CCTACGGGAGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	282	0.39689241682148285	No Hit
CCTACGGGCGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	246	0.3462252997804425	No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	181	0.2547430051230085	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	181	0.2547430051230085	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	176	0.24770590553397512	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	167	0.23503912627371504	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	164	0.23081686652029498	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	161	0.22659460676687496	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	160	0.2251871868490683	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	149	0.20970556775319485	No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	133	0.18718684906828803	No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	130	0.18296458931486798	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	126	0.17733490964364126	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	126	0.17733490964364126	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	124	0.17452006980802792	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	117	0.16466813038338118	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	102	0.14355683161628102	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	94	0.1322974722738276	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	94	0.1322974722738276	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	88	0.12385295276698756	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	88	0.12385295276698756	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	85	0.11963069301356753	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	77	0.10837133367111412	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	76	0.10696391375330744	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	5.629679671226707E-4	0.0	0.0	0.0
160-164	0.0	0.0014074199178066768	0.0	0.0	0.0
165-169	0.0	0.0014074199178066768	0.0	0.0	0.0
170-174	0.0	0.0014074199178066768	0.0	0.0	0.0
175-179	0.0	0.0014074199178066768	0.0	0.0	0.0
180-184	0.0	0.0014074199178066768	0.0	0.0	0.0
185-189	0.0	0.0014074199178066768	0.0	0.0	0.0
190-194	0.0	0.0014074199178066768	0.0	0.0	0.0
195-199	0.0	0.0014074199178066768	0.0	0.0	0.0
200-204	0.0	0.0014074199178066768	0.0	0.0	0.0
205-209	0.0	0.0014074199178066768	0.0	0.0	0.0
210-214	0.0	0.0014074199178066768	0.0	0.0	0.0
215-219	0.0	0.0014074199178066768	0.0	0.0	0.0
220-224	0.0	0.0014074199178066768	0.0	0.0	0.0
225-229	0.0	0.0014074199178066768	0.0	0.0	0.0
230-234	0.0	0.0014074199178066768	0.0	0.0	0.0
235-239	0.0	0.0014074199178066768	0.0	0.0	0.0
240-244	0.0	0.0014074199178066768	0.0	0.0	0.0
245-249	0.0	0.0014074199178066768	0.0	0.0	0.0
250-254	0.0	0.0014074199178066768	0.0	0.0	0.0
255-259	0.0	0.0014074199178066768	0.0	0.0	0.0
260-264	0.0	0.0014074199178066768	0.0	0.0	0.0
265-269	0.0	0.0014074199178066768	0.0	0.0	0.0
270-274	0.0	0.0014074199178066768	0.0	0.0	0.0
275-279	0.0	0.0014074199178066768	0.0	0.0	0.0
280-284	0.0	0.0014074199178066768	0.0	0.0	0.0
285-289	0.0	0.0014074199178066768	0.0	0.0	0.0
290	0.0	0.0014074199178066768	0.0	0.0	0.0
>>END_MODULE
