##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	DAFS1_cleaned_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	89069
Sequences flagged as poor quality	0
Sequence length	76-301
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.677014449471756	40.0	40.0	40.0	40.0	40.0
2	39.13954349998316	40.0	40.0	40.0	40.0	40.0
3	39.19653302495818	40.0	40.0	40.0	40.0	40.0
4	39.21723607540222	40.0	40.0	40.0	40.0	40.0
5	39.3035511794227	40.0	40.0	40.0	40.0	40.0
6	39.380345574779106	40.0	40.0	40.0	40.0	40.0
7	39.32376023083228	40.0	40.0	40.0	40.0	40.0
8	39.41079387890287	40.0	40.0	40.0	40.0	40.0
9	39.07119199721564	40.0	40.0	40.0	40.0	40.0
10-14	39.60223197745568	40.0	40.0	40.0	40.0	40.0
15-19	39.50050410356016	40.0	40.0	40.0	40.0	40.0
20-24	39.45348437728054	40.0	40.0	40.0	40.0	40.0
25-29	39.63772805353153	40.0	40.0	40.0	40.0	40.0
30-34	39.51467738494875	40.0	40.0	40.0	40.0	40.0
35-39	39.62647385734655	40.0	40.0	40.0	40.0	40.0
40-44	39.583289359934426	40.0	40.0	40.0	40.0	40.0
45-49	39.52188078905119	40.0	40.0	40.0	40.0	40.0
50-54	39.523722058179615	40.0	40.0	40.0	40.0	40.0
55-59	39.447870751888985	40.0	40.0	40.0	40.0	40.0
60-64	39.472929975636866	40.0	40.0	40.0	40.0	40.0
65-69	39.50956674039228	40.0	40.0	40.0	40.0	40.0
70-74	39.44482592147661	40.0	40.0	40.0	40.0	40.0
75-79	39.45020160816575	40.0	40.0	40.0	40.0	40.0
80-84	39.43253651909322	40.0	40.0	40.0	40.0	40.0
85-89	39.607744506776115	40.0	40.0	40.0	40.0	40.0
90-94	39.48419492476982	40.0	40.0	40.0	40.0	40.0
95-99	39.348511116101506	40.0	40.0	40.0	40.0	40.0
100-104	39.29911520323377	40.0	40.0	40.0	40.0	40.0
105-109	39.39809904726968	40.0	40.0	40.0	40.0	40.0
110-114	39.40001524771154	40.0	40.0	40.0	40.0	40.0
115-119	39.341165373429305	40.0	40.0	40.0	40.0	40.0
120-124	39.254897645222506	40.0	40.0	40.0	40.0	40.0
125-129	39.38018120489805	40.0	40.0	40.0	40.0	40.0
130-134	39.37262916755567	40.0	40.0	40.0	40.0	40.0
135-139	39.14318935303279	40.0	40.0	40.0	40.0	40.0
140-144	39.4440849468656	40.0	40.0	40.0	40.0	40.0
145-149	39.117663168848516	40.0	40.0	40.0	40.0	40.0
150-154	39.272090202596466	40.0	40.0	40.0	40.0	40.0
155-159	39.195380558953666	40.0	40.0	40.0	40.0	40.0
160-164	39.30561863999875	40.0	40.0	40.0	40.0	40.0
165-169	39.29316157725093	40.0	40.0	40.0	40.0	40.0
170-174	39.11131517000868	40.0	40.0	40.0	40.0	40.0
175-179	39.359448317906356	40.0	40.0	40.0	40.0	40.0
180-184	39.32537232967181	40.0	40.0	40.0	40.0	40.0
185-189	39.23087850704532	40.0	40.0	40.0	40.0	40.0
190-194	39.35521683137989	40.0	40.0	40.0	40.0	40.0
195-199	39.292931689322316	40.0	40.0	40.0	40.0	40.0
200-204	39.45142380012282	40.0	40.0	40.0	40.0	40.0
205-209	39.261113728691946	40.0	40.0	40.0	40.0	40.0
210-214	39.333099553840114	40.0	40.0	40.0	40.0	40.0
215-219	39.369522040913175	40.0	40.0	40.0	40.0	40.0
220-224	39.26879845396733	40.0	40.0	40.0	40.0	40.0
225-229	39.322466046776995	40.0	40.0	40.0	40.0	40.0
230-234	39.199217986314764	40.0	40.0	40.0	40.0	40.0
235-239	39.30262356602735	40.0	40.0	40.0	40.0	40.0
240-244	39.27837589897045	40.0	40.0	40.0	40.0	40.0
245-249	39.15633808424309	40.0	40.0	40.0	40.0	40.0
250-254	39.28857627613793	40.0	40.0	40.0	40.0	40.0
255-259	39.08620995741525	40.0	40.0	40.0	40.0	40.0
260-264	39.021856876735605	40.0	40.0	40.0	40.0	40.0
265-269	39.13712076947279	40.0	40.0	40.0	40.0	40.0
270-274	38.130145175064044	40.0	36.8	40.0	33.6	40.0
275-279	39.05109275802011	40.0	40.0	40.0	40.0	40.0
280-284	38.44675318838137	40.0	40.0	40.0	36.8	40.0
285-289	39.0285953368485	40.0	40.0	40.0	40.0	40.0
290-294	39.077479380632404	40.0	40.0	40.0	40.0	40.0
295-299	38.98863323324947	40.0	40.0	40.0	40.0	40.0
300-301	38.41685557857515	40.0	40.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.001298121823978704
1101	2	-0.10122279862851258
1101	3	0.02474539034483314
1101	4	0.44871541532128845
1101	5	0.20492494381159787
1101	6	0.06293063640643481
1101	7	0.015719060331498724
1101	8	-0.19308168993485708
1101	9	0.38493185919870143
1101	10-14	0.08144582763668495
1101	15-19	0.0697989780389463
1101	20-24	-0.11518912114707547
1101	25-29	0.02161367965721439
1101	30-34	0.07752341604667379
1101	35-39	0.02435894691246432
1101	40-44	0.06657172623646801
1101	45-49	-0.18846008354229582
1101	50-54	0.06174177451067209
1101	55-59	-0.09934558249861425
1101	60-64	-0.0472193845577209
1101	65-69	-0.026688627190829095
1101	70-74	-0.11963267069479144
1101	75-79	0.00934296387458744
1101	80-84	0.1097119539603284
1101	85-89	0.02753462589575406
1101	90-94	-0.09089404065872486
1101	95-99	0.02210872900742089
1101	100-104	0.03147990095736475
1101	105-109	-0.051899403605759176
1101	110-114	0.14701185937281736
1101	115-119	0.18387578437989305
1101	120-124	0.1626713254860377
1101	125-129	0.10692369118823564
1101	130-134	0.05522386289153047
1101	135-139	-0.017601915812235802
1101	140-144	0.08547490728794571
1101	145-149	0.22057495449699616
1101	150-154	0.07329771411031771
1101	155-159	0.10144567788077552
1101	160-164	0.07386668571557209
1101	165-169	0.07112605287976237
1101	170-174	0.18564422079228393
1101	175-179	0.19519367862321246
1101	180-184	0.06555426276259624
1101	185-189	0.16549208252520486
1101	190-194	0.12840361956863688
1101	195-199	0.0795593933948382
1101	200-204	0.1263934186022979
1101	205-209	0.03383561964713522
1101	210-214	0.13862475581445466
1101	215-219	0.06688631417903679
1101	220-224	0.08204004997232772
1101	225-229	0.013488208135164825
1101	230-234	0.15512367741779798
1101	235-239	0.06088597999809764
1101	240-244	0.04968730429143875
1101	245-249	-0.02363963844364747
1101	250-254	0.03611236030230458
1101	255-259	0.07161708981812609
1101	260-264	0.1603104686629706
1101	265-269	0.14076156548701135
1101	270-274	0.4196291794256197
1101	275-279	0.135635147725921
1101	280-284	0.13590203546688429
1101	285-289	0.171378686081475
1101	290-294	0.0013094647310296637
1101	295-299	-0.025129004614782957
1101	300-301	0.4024178308828681
1102	1	-0.2357301412629127
1102	2	-0.40839879310092186
1102	3	0.050189845756719365
1102	4	-0.3859065919391611
1102	5	0.07739557849721734
1102	6	-0.14058113713790732
1102	7	-0.23683909450679153
1102	8	-0.12811058913313644
1102	9	0.11627350584979723
1102	10-14	-0.00660953598871572
1102	15-19	-0.0013665315630433383
1102	20-24	-0.15336457821504013
1102	25-29	0.07237098122885754
1102	30-34	-0.1663309802507129
1102	35-39	-0.07359343247314598
1102	40-44	-0.06742404033258964
1102	45-49	-0.051428530224100655
1102	50-54	-0.13321539764518775
1102	55-59	-0.20275535887256524
1102	60-64	-0.2836422450848062
1102	65-69	-0.0038412610209945797
1102	70-74	-0.15163126686276485
1102	75-79	-0.054329592306118
1102	80-84	-0.1334405263256926
1102	85-89	0.06309544582139637
1102	90-94	-0.07870702391144846
1102	95-99	-0.02215584945910365
1102	100-104	0.02492576020126336
1102	105-109	-0.022146938674140415
1102	110-114	-0.20055062760334863
1102	115-119	-0.013660929117726539
1102	120-124	-0.26181238238529403
1102	125-129	0.053411369272005516
1102	130-134	-0.014599232338717627
1102	135-139	0.0527827122271205
1102	140-144	-0.009143371043691673
1102	145-149	-0.13891133072218054
1102	150-154	-0.08588806331649579
1102	155-159	-0.09472229939468946
1102	160-164	-0.1819992483262638
1102	165-169	1.0092648004444982E-4
1102	170-174	-0.11171372347120467
1102	175-179	-0.1677407690017887
1102	180-184	-0.06575667506299965
1102	185-189	-0.0549446408431109
1102	190-194	-0.07191443612400406
1102	195-199	-0.04557468353077354
1102	200-204	0.004575894830381344
1102	205-209	-0.0134471976682633
1102	210-214	0.004681632946258674
1102	215-219	0.017383687477725118
1102	220-224	0.04272397938589734
1102	225-229	0.06564056777936855
1102	230-234	-0.061303350735620654
1102	235-239	-0.0017073803151248512
1102	240-244	-0.058670979962606395
1102	245-249	-0.038309683081124035
1102	250-254	8.937244322098081E-4
1102	255-259	-0.07100346974053906
1102	260-264	-0.02766263917984446
1102	265-269	-0.16710757104662122
1102	270-274	-0.09986763453373726
1102	275-279	-0.06457762056502503
1102	280-284	-0.19649906566385766
1102	285-289	-0.13098918371310475
1102	290-294	-0.05207124793307116
1102	295-299	0.06566380153961404
1102	300-301	0.047073212405884135
1103	1	0.09758009044637816
1103	2	-0.3088307934705483
1103	3	0.26688307929103416
1103	4	-0.03942843452469447
1103	5	0.23587271944022348
1103	6	0.061927699233287115
1103	7	0.27920498339188526
1103	8	-0.22116393078305663
1103	9	-0.41039731910920096
1103	10-14	0.020983043769639664
1103	15-19	0.0030320176549665234
1103	20-24	0.019491013533055934
1103	25-29	0.022416029395728287
1103	30-34	0.02892394617060745
1103	35-39	0.0850223182141292
1103	40-44	-0.00965149892292061
1103	45-49	-0.07464104206368205
1103	50-54	0.016982121240381787
1103	55-59	-0.06381295122130837
1103	60-64	-0.05830900415796947
1103	65-69	-0.07818228719512632
1103	70-74	-0.03475553049849367
1103	75-79	-0.10086550378068893
1103	80-84	-0.27762238231017733
1103	85-89	-0.031953876145934146
1103	90-94	-0.03278099531163292
1103	95-99	0.010703900581326309
1103	100-104	-0.055689667063276715
1103	105-109	-0.09469468689471938
1103	110-114	-0.0636547114361008
1103	115-119	-0.08918459401856893
1103	120-124	-0.20278633534529433
1103	125-129	0.05185341547739597
1103	130-134	-0.12848750943193465
1103	135-139	0.01480758848339292
1103	140-144	-0.11411050795583577
1103	145-149	-0.14633015831558538
1103	150-154	-0.22751348207454214
1103	155-159	-0.17172972981420997
1103	160-164	-0.25495414417287776
1103	165-169	-0.21468340588342727
1103	170-174	-0.17940125172650312
1103	175-179	-0.2408440414002655
1103	180-184	-0.09152782741558241
1103	185-189	-0.23835660602098585
1103	190-194	-0.2629061909582546
1103	195-199	-0.16777105393560987
1103	200-204	-0.04769027548140059
1103	205-209	-0.24631830050679326
1103	210-214	-0.20485768766798174
1103	215-219	-0.12427289698017319
1103	220-224	-0.14496331703103493
1103	225-229	-0.26543435078740174
1103	230-234	-0.3120432297491078
1103	235-239	-0.1321587530466246
1103	240-244	-0.14548345087931125
1103	245-249	-0.21401000881402865
1103	250-254	-0.1850997609098073
1103	255-259	-0.21540551720447354
1103	260-264	-0.2647496563971572
1103	265-269	-0.25653521180976924
1103	270-274	-0.4060658662694294
1103	275-279	-0.32518255309177846
1103	280-284	-0.11738319781835571
1103	285-289	-0.14274348804070058
1103	290-294	-0.18033555691398817
1103	295-299	-0.017788937274715977
1103	300-301	-0.43682592494475614
1104	1	0.10535236286452232
1104	2	0.15614540394516752
1104	3	0.162054555051256
1104	4	-0.050892536341464734
1104	5	-0.10474711928512193
1104	6	0.20416028448539691
1104	7	0.22382754241257885
1104	8	-0.0697017720343922
1104	9	-0.32315356121548433
1104	10-14	0.034584520501397265
1104	15-19	0.11953838120301441
1104	20-24	-0.05813455724319283
1104	25-29	-0.10232894291554118
1104	30-34	0.06735783327835065
1104	35-39	-0.11045033310229968
1104	40-44	-0.13148186907739756
1104	45-49	-0.05227632818046146
1104	50-54	-0.022073547660809822
1104	55-59	0.012549625287000765
1104	60-64	-0.010606682191593109
1104	65-69	-0.019521061119164074
1104	70-74	0.008147413249673718
1104	75-79	-0.1612949218317965
1104	80-84	-0.12000069767012178
1104	85-89	-0.15965231197611018
1104	90-94	-0.20825947583237792
1104	95-99	-0.3148960216959509
1104	100-104	-0.24863632984380502
1104	105-109	-0.09356722335576961
1104	110-114	-0.07986881956265535
1104	115-119	-0.09310405972323821
1104	120-124	-0.23610179383503294
1104	125-129	-0.17042513958524808
1104	130-134	-0.10533146204734578
1104	135-139	-0.12857465692196257
1104	140-144	-0.015470621054845424
1104	145-149	-0.18770879625250103
1104	150-154	-0.3074042830300385
1104	155-159	-0.11871944365421427
1104	160-164	-0.18066954416313763
1104	165-169	-0.06276998512586118
1104	170-174	-0.2868063119322244
1104	175-179	-0.10853769908077027
1104	180-184	-0.20514358327772442
1104	185-189	-0.12136737967672673
1104	190-194	-0.12108671759293088
1104	195-199	-0.057399233974742
1104	200-204	-0.2068292187299221
1104	205-209	-0.0878838582367365
1104	210-214	-0.16904555459557713
1104	215-219	-0.27421418856529556
1104	220-224	-0.10962085127761156
1104	225-229	-0.07968092612986055
1104	230-234	-0.12699430607004558
1104	235-239	-0.16922333230298392
1104	240-244	-0.20567914559555334
1104	245-249	-0.1556538444304678
1104	250-254	-0.1974285280331003
1104	255-259	-0.2958751845234602
1104	260-264	-0.19292969553610106
1104	265-269	-0.07684832335575464
1104	270-274	-0.07029287214027136
1104	275-279	-0.2837735511348427
1104	280-284	-0.19757889253460093
1104	285-289	-0.23679436866692072
1104	290-294	-0.18980718118013584
1104	295-299	0.0754791644865378
1104	300-301	-0.6872844180660351
1201	1	0.17737325442084995
1201	2	0.07917651220557786
1201	3	-0.3312335743297794
1201	4	0.2907027355694751
1201	5	-0.15303593588841125
1201	6	-0.09391091173945654
1201	7	-0.43480625975904275
1201	8	0.3339725762498418
1201	9	0.20133445820999896
1201	10-14	0.2174772680159478
1201	15-19	0.11297769947854164
1201	20-24	0.14613538365569667
1201	25-29	-0.06428431928426903
1201	30-34	0.08042421894747065
1201	35-39	-0.05889950156338131
1201	40-44	0.023537144071440252
1201	45-49	0.03553234914578951
1201	50-54	0.06184087026194618
1201	55-59	-0.02533006500484447
1201	60-64	0.05834461665845936
1201	65-69	0.035795748337022815
1201	70-74	0.10175624634630509
1201	75-79	0.036936626250856364
1201	80-84	0.005733484764476771
1201	85-89	-0.002085995029212029
1201	90-94	0.06037111930208283
1201	95-99	0.042450384131690555
1201	100-104	-0.11916365838185072
1201	105-109	-0.11160909809806441
1201	110-114	-0.06937722658148715
1201	115-119	-0.0643140290273152
1201	120-124	-0.09840994429088568
1201	125-129	-0.22406512675710388
1201	130-134	-0.07202409044337088
1201	135-139	-0.16421858416803303
1201	140-144	-0.013152391339353642
1201	145-149	-0.17645667731724046
1201	150-154	-0.12046150573585379
1201	155-159	-0.15341958568014036
1201	160-164	-0.21359207130841185
1201	165-169	-0.28325837106986995
1201	170-174	-0.1253182466919327
1201	175-179	-0.16294737517001323
1201	180-184	-0.04809955523905529
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2404	200-204	0.3060157286593892
2404	205-209	0.023412548354492912
2404	210-214	0.24628102868811652
2404	215-219	0.14019915270927186
2404	220-224	0.05416711775156813
2404	225-229	0.2777540550097086
2404	230-234	0.26327768122397543
2404	235-239	0.20651619519352948
2404	240-244	0.163667687837048
2404	245-249	0.2320769477077107
2404	250-254	0.32069734053946775
2404	255-259	0.16852823020752794
2404	260-264	0.32344910136681904
2404	265-269	0.2926573347740842
2404	270-274	0.19629438217777562
2404	275-279	0.2795379438026302
2404	280-284	0.2384346075646704
2404	285-289	0.2933227645472982
2404	290-294	0.27034767289966766
2404	295-299	0.3029812490606929
2404	300-301	0.15056828560400248
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	24.0
22	40.0
23	60.0
24	121.0
25	186.0
26	188.0
27	178.0
28	205.0
29	259.0
30	251.0
31	311.0
32	414.0
33	453.0
34	601.0
35	800.0
36	1073.0
37	1754.0
38	3586.0
39	70220.0
40	8341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.06624077962029438	0.016840876174651114	0.06848622977691453	99.84843211442815
2	0.01347270093972089	0.011227250783100742	0.19310871346933275	99.78219133480785
3	0.01347270093972089	0.12349975861410817	99.62613254892274	0.23689499152342564
4	0.08195893071663542	99.4981418899954	0.11227250783100741	0.3076266714569603
5	0.19198598839102268	0.14258608494537944	0.07634530532508504	99.5890826213385
6	99.7664731837115	0.06511805454198431	0.021331776487891408	0.14707698525861973
7	99.86751844075941	0.08644983102987572	0.03704992758423245	0.008981800626480593
8	99.70360057932615	0.11114978275269735	0.1133952329093175	0.07185440501184474
9	29.333438121007312	22.96758692698919	25.53862735631926	22.160347595684247
10-14	59.81340309198486	10.117998405730388	9.910967901290011	20.157630600994732
15-19	38.127743659410115	21.475036207883775	20.017963601252962	20.379256531453144
20-24	60.26406493841853	39.21207154004199	0.45627547182521416	0.06758804971426648
25-29	17.75522347842684	18.031413847691116	60.388238332079624	3.825124341802423
30-34	3.9764676823586207	57.2115999955091	0.5261089716961008	38.285823350436175
35-39	62.528152331338624	2.3795035309703714	19.891095667403924	15.201248470287082
40-44	35.450268892656254	47.68976860636136	0.21691048512950634	16.643052015852877
45-49	20.11900885830087	19.907711998562913	22.153835790230048	37.81944335290618
50-54	40.552380738528555	20.31660847208344	17.527759377561217	21.603251411826786
55-59	23.713750014034062	25.16790353546127	12.592709023341456	38.52563742716321
60-64	39.86931480088471	0.287866710078703	0.47491270812516134	59.36790578091142
65-69	29.93970966329475	22.304505495739257	44.60519372621226	3.15059111475373
70-74	40.34467659904119	57.78665978061952	1.7182184598457377	0.15044516049354995
75-79	38.15533304740112	22.445528791202868	9.861517491640974	29.537620669755032
80-84	39.65979138362732	1.0206258491180402	38.51161537338738	20.807967393867262
85-89	33.77729272351233	5.545787101598658	55.668285759839755	5.008634415049258
90-94	16.891309229732766	58.86683134965192	14.253986076802155	9.98787334381316
95-99	5.091174489108466	14.060184145519875	60.46103750280709	20.387603862564564
100-104	29.635751178980463	22.873568380866832	10.564338648102403	36.9263417920503
105-109	66.72902275360887	30.278783360132227	1.5450535828175669	1.4471403034413375
110-114	45.86814351677177	48.79489390754415	2.8245656587434196	2.5123969169406566
115-119	37.17583910704862	37.117896084354264	19.15129192727926	6.554972881317866
120-124	24.770642201834864	31.873378774437693	32.76408430934387	10.591894714383569
125-129	12.790083973597389	44.315555665354324	23.590090262072184	19.3042700989761
130-134	22.013003784348296	27.716252484531335	36.053048253248136	14.21769547787223
135-139	8.892512313279475	19.531006106667924	27.769505290274477	43.80697628977813
140-144	39.74207227097235	18.3541048555726	19.083372825054294	22.820450048400755
145-149	1.933840638953525	29.87570413361244	33.220731849748894	34.96972337768514
150-154	36.205920668433585	14.153227617806927	13.100489645568484	36.54036206819101
155-159	26.197444403765335	62.11280291272385	0.2886220040025246	11.401130679508286
160-164	26.91371224236761	14.53769923271819	1.1430612208226267	57.40552730409158
165-169	35.403016587864016	25.329454970182276	24.562392605653574	14.705135836300132
170-174	7.083637507159785	29.7155179191141	20.09973157829715	43.10111299542897
175-179	53.922856956733675	4.490389129619272	16.28760055079711	25.299153362849946
180-184	34.986185052901135	38.026371947795226	1.6537502527124468	25.33369274659119
185-189	35.33188365426951	5.770561331308575	19.23572859851378	39.66182641590813
190-194	25.23752075287732	31.530220008581978	28.34882357302205	14.88343566551866
195-199	27.17285073972972	34.89861714914794	20.102674709893392	17.82585740122895
200-204	69.27373548549448	2.3683808462451017	24.804750692022864	3.5531329762375523
205-209	23.471348098748678	41.65181811440987	9.225051623529094	25.651782163312355
210-214	38.94034586276942	30.86901616063564	28.790064534685143	1.4005734419097953
215-219	39.9052181689894	18.86491004939082	19.619479486407414	21.61039229521237
220-224	20.087413765982788	29.668097346127055	31.571650075279205	18.672838812610955
225-229	49.18900334376011	7.906599431920325	21.79758566138137	21.106811562938194
230-234	20.665161065606004	52.125481736160275	20.320445837687217	6.888911360546511
235-239	43.764676801384255	22.305592071999193	7.504185346232065	26.42554578038449
240-244	34.30816041276219	30.54008746028108	10.06242668572276	25.089325441233974
245-249	45.58559813751972	9.691728951590793	18.111991514568604	26.610681396320885
250-254	30.369554714097912	4.622299126956483	53.206215800177524	11.801930358768075
255-259	13.681502039348755	39.460668097394354	16.546927493567342	30.310902369689547
260-264	39.87379463312576	28.20474213969334	20.893100728332612	11.028362498848287
265-269	21.99020180682278	47.56528383298126	19.24828082160996	11.196233538586004
270-274	12.684390309676841	5.6087914063553415	29.354802463032048	52.35201582093577
275-279	40.13731108489241	20.802516995336816	18.868700488791504	20.19147143097927
280-284	8.665205910444406	34.47407157705489	14.988033035563797	41.872689476936905
285-289	46.23639240221857	19.45807826110407	25.240689301798763	9.0648400348786
290-294	28.837289788726856	20.975169056720805	22.17615159707755	28.011389557474793
295-299	31.72341943192481	24.185161100147837	27.803417091759243	16.288002376168116
300-301	24.316045629101882	48.16524950224345	27.439290718187543	0.07941415046712537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.25
39	0.3333333333333333
40	0.6666666666666666
41	0.3333333333333333
42	0.25
43	2.6666666666666665
44	3.5833333333333335
45	52.916666666666764
46	124.16666666666603
47	41.33333333333335
48	58.83333333333334
49	264.75
50	1147.0833333333335
51	982.4166666666671
52	562.9166666666667
53	1415.5833333333335
54	8991.833333333328
55	13668.083333333376
56	2983.250000000004
57	334.9166666666667
58	81.5
59	27.499999999999996
60	7.749999999999999
61	3.4166666666666665
62	4.5
63	3.333333333333333
64	10.166666666666668
65	1.3333333333333333
66	0.3333333333333333
67	0.0
68	1.25
69	1.0
70	0.5
71	1.3333333333333333
72	0.5
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.25
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	2.2473425174730882E-4
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	6.0
80-84	0.0
85-89	3.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	4.0
110-114	3.0
115-119	0.0
120-124	0.0
125-129	2.0
130-134	0.0
135-139	1.0
140-144	3.0
145-149	3.0
150-154	0.0
155-159	1.0
160-164	2.0
165-169	0.0
170-174	6.0
175-179	1.0
180-184	0.0
185-189	5.0
190-194	8.0
195-199	6.0
200-204	2.0
205-209	4.0
210-214	4.0
215-219	3.0
220-224	0.0
225-229	1.0
230-234	0.0
235-239	0.0
240-244	1.0
245-249	1.0
250-254	0.0
255-259	0.0
260-264	3.0
265-269	0.0
270-274	1.0
275-279	0.0
280-284	0.0
285-289	1.0
290-294	0.0
295-299	661.0
300-302	88333.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	5.466548406291751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.36147052782912	3.2996890051533083
2	14.294516327788045	1.5628333090076232
3	5.976586568083795	0.9801389933646949
4	3.8406243581844324	0.8397983585759355
5	2.485109878825221	0.6792486723775949
6	1.8073526391456152	0.5927988413477192
7	1.0269049086054631	0.392953777408526
8	1.088519203121791	0.47603543320347147
9	0.7804477305401519	0.38397197678204537
>10	6.777572396796057	7.196667751967575
>50	0.24645717806531117	0.8117302316181836
>100	0.7599096323680428	11.438323097823035
>500	0.20538098172109262	8.116179591103528
>1k	0.2259190798932019	24.1060301563956
>5k	0.12322858903265559	39.123600803871156
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	6634	7.448158169509032	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	6049	6.791363998697639	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	5775	6.483737327240678	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	5599	6.286137713458105	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	5547	6.227756009385982	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	5243	5.886447585579719	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	4532	5.088190054901257	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	4501	5.053385577473644	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1696	1.9041417328138859	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1551	1.7413465964589252	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1493	1.676228541916941	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1447	1.6245831883146773	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1413	1.5864105356521347	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1370	1.5381333572848015	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1245	1.3977927224960425	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1204	1.3517609942853293	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1019	1.1440568547979657	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	964	1.0823069754909116	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	841	0.9442117908587724	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	838	0.9408436156238422	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	786	0.8824619115517183	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	767	0.8611301350638269	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	709	0.7960120805218426	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	694	0.7791712043471916	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	572	0.6421987447933625	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	537	0.6029033670525099	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	521	0.5849397657995486	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	493	0.5535034636068666	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	490	0.5501352883719364	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	458	0.514208085866014	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	431	0.483894508751642	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	370	0.41540827897472743	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	355	0.3985674028000764	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	334	0.3749901761555648	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	328	0.36825382568570436	No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	324	0.3637629253724641	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	319	0.3581492999809137	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	313	0.35141294951105323	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	307	0.3446765990411928	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	307	0.3446765990411928	No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	288	0.3233448225533014	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	288	0.3233448225533014	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	286	0.32109937239668124	No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	260	0.29190852036061926	No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	253	0.28404944481244876	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	252	0.2829267197341387	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	246	0.27619036926427826	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	246	0.27619036926427826	No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	240	0.2694540187944178	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	240	0.2694540187944178	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	239	0.2683312937161077	No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	235	0.2638403934028674	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	222	0.24924496738483645	No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	221	0.2481222423065264	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	212	0.23801771660173573	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	210	0.2357722664451156	No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	203	0.22791319089694506	No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	190	0.2133177648789141	No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	189	0.212195039800604	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	187	0.20994958964398389	No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	174	0.1953541636259529	No Hit
CCTACGGGCGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	171	0.19198598839102268	No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	160	0.17963601252961187	No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	147	0.1650405865115809	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
