FastQCFastQC Report
Fri 1 Nov 2024
CVMS1_cleaned_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCVMS1_cleaned_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182802
Sequences flagged as poor quality0
Sequence length16-301
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC45922.5120075272699425No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC44122.4135403332567478No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC42762.339142897780112No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC42292.3134320193433333No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC41852.2893622608067745No Hit
CCTACGGGCGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC41132.249975383201497No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT38962.131267710418923No Hit
CCTACGGGCGGCAGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC37502.0513998752748877No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT36932.0202185971707096No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT36882.0174833973370094No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT34221.8719707661841773No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT34061.863218126716338No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT34041.862124046782858No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC33731.8451658078139188No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC31851.7423222940668046No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT30741.681600857758668No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC30571.6723011783240884No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC30371.6613603789892888No Hit
CCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC29901.6356495005525102No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC29681.623614621284231No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC29181.5962626229472323No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC29031.5880570234461329No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC29001.586415903545913No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC27711.515847747836457No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC27641.5120184680692772No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC27181.4868546295992384No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC26891.4709904705637795No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC26091.4272272732245819No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT25461.3927637553199637No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC23521.2866380017724095No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC21271.1635540092559162No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC20051.09681513331364No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC11820.6466012406866446No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC11050.6044791632476669No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC10780.5897090841456877No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT10780.5897090841456877No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT10700.585332764411768No Hit
CCTACGGGCGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC10500.5743919650769685No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC10460.5722038052100086No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC10400.5689215654095688No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT10260.5612630058752092No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT10260.5612630058752092No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC9770.5344580475049507No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT9670.528987647837551No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT9630.5267994879705912No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT9200.5032767694007724No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC6940.3796457369175392No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT6860.3752694171836194No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT6640.3632345379153401No Hit
CCTACGGGTGGCTGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC6580.35995229811490026No Hit
CCTACGGGTGGCAGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC6070.3320532598111618No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT6000.328223980043982No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT5910.3233006203433223No Hit
CCTACGGGGGGCAGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC5850.32001838054288245No Hit
CCTACGGGAGGCAGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC5720.3129068609752629No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT5680.31071870110830296No Hit
CCTACGGGCGGCTGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC5570.3047012614741633No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT5540.3030601415739434No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT5530.3025131016072034No Hit
CCTACGGGGGGCTGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC5380.29430750210610385No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC5160.28227262283782456No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC5130.2806315029376046No Hit
CCTACGGGCGGCAGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC5080.2778963031039048No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4880.2669555037691054No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT4860.2658614238356254No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT4780.26148510410170567No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4590.25109134473364625No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4370.23905646546536688No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC4220.23085086596426732No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4110.22483342633012768No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC4090.22373934639664775No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3910.21389262699532827No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3880.21225150709510837No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3800.20787518736118862No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3740.2045929475607488No Hit
CCTACGGGAGGCTGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC3730.2040459075940088No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3730.2040459075940088No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3550.19419918819268936No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3460.18927582849202965No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3340.18271134889114998No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3320.18161726895767005No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3250.17778798919049027No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3220.17614686929027035No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3180.1739587094233105No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3100.16958238968939068No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3080.16848830975591075No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2970.16247087012177108No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2940.1608297502215512No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2930.16028271025481122No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2920.15973567028807126No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2920.15973567028807126No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2830.15481231058741152No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2790.15262415072045166No Hit
CCTACGGGGGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC2620.14332447128587214No Hit
CCTACGGGCGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC2410.13183663198433276No Hit
CCTACGGGTGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC2370.1296484721173729No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2320.12691327228367305No Hit
CCTACGGGAGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC2300.1258191923501931No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2250.12308399251649325No Hit
CCTACGGGGGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC2230.12198991258301332No Hit
CCTACGGGTGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC2200.12034879268279342No Hit
CCTACGGGTGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA2150.11761359284909356No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTGCGCAATGGGCGAAAGCCTGAC2050.11214319318169384No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTGCGCAATGGGCGAAAGCCTGAC2030.11104911324821391No Hit
CCTACGGGTGGCTGCAGTAGGGAATTTTGCGCAATGGGCGAAAGCCTGAC1970.1077668734477741No Hit
CCTACGGGCGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC1950.10667279351429415No Hit
CCTACGGGCGGCTGCAGTAGGGAATTTTGCGCAATGGGCGAAAGCCTGAC1910.10448463364733428No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT1870.1022964737803744No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGGAACTCTGAC1850.10120239384689446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)